DIsEMBL depends on BioPython and Tisean sav_gol executable both has to be installed DisEMBL.py script changes: move import sys statement up to append Bio python libraries path change path to python interpreter Comment out file argument read data from stdin instead was: file = str(sys.argv[7]) db = open(file,'r') become: # file = str(sys.argv[7]) db = sys.stdin Header print ' ____ _ _____ __ __ ____ _ _ _ _' print '| _ \(_)___| ____| \/ | __ )| | / || || |' print '| | | | / __| _| | |\/| | _ \| | | || || |_' print '| |_| | \__ \ |___| | | | |_) | |___ | ||__ _|' print '|____/|_|___/_____|_| |_|____/|_____| |_(_) |_|' print '# Copyright (C) 2004 - Rune Linding & Lars Juhl Jensen ' print '# EMBL Biocomputing Unit - Heidelberg - Germany ' print '' removed to facilitate parsing