/*
* Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
* Copyright (C) $$Year-Rel$$ The Jalview Authors
*
* This file is part of Jalview.
*
* Jalview is free software: you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation, either version 3
* of the License, or (at your option) any later version.
*
* Jalview is distributed in the hope that it will be useful, but
* WITHOUT ANY WARRANTY; without even the implied warranty
* of MERCHANTABILITY or FITNESS FOR A PARTICULAR
* PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Jalview. If not, see .
* The Jalview Authors are detailed in the 'AUTHORS' file.
*/
package jalview.ext.ensembl;
import jalview.datamodel.Alignment;
import jalview.datamodel.AlignmentI;
import jalview.io.FeaturesFile;
import jalview.io.FileParse;
import java.io.IOException;
import java.net.MalformedURLException;
import java.net.URL;
import java.util.ArrayList;
import java.util.List;
/**
* A client for fetching and processing Ensembl feature data in GFF format by
* calling the overlap REST service
*
* @author gmcarstairs
* @see http://rest.ensembl.org/documentation/info/overlap_id
*/
class EnsemblFeatures extends EnsemblRestClient
{
/*
* The default features to retrieve from Ensembl
* can override in getSequenceRecords parameter
*/
private EnsemblFeatureType[] featuresWanted = { EnsemblFeatureType.cds,
EnsemblFeatureType.exon, EnsemblFeatureType.variation };
/**
* Default constructor (to use rest.ensembl.org)
*/
public EnsemblFeatures()
{
super();
}
/**
* Constructor given the target domain to fetch data from
*
* @param d
*/
public EnsemblFeatures(String d)
{
super(d);
}
@Override
public String getDbName()
{
return "ENSEMBL (features)";
}
/**
* Makes a query to the REST overlap endpoint for the given sequence
* identifier. This returns an 'alignment' consisting of one 'dummy sequence'
* (the genomic sequence for which overlap features are returned by the
* service). This sequence will have on it sequence features which are the
* real information of interest, such as CDS regions or sequence variations.
*/
@Override
public AlignmentI getSequenceRecords(String query) throws IOException
{
// TODO: use a vararg String... for getSequenceRecords instead?
List queries = new ArrayList<>();
queries.add(query);
FileParse fp = getSequenceReader(queries);
if (fp == null || !fp.isValid())
{
return null;
}
FeaturesFile fr = new FeaturesFile(fp);
return new Alignment(fr.getSeqsAsArray());
}
/**
* Returns a URL for the REST overlap endpoint
*
* @param ids
* @return
*/
@Override
protected URL getUrl(List ids) throws MalformedURLException
{
StringBuffer urlstring = new StringBuffer(128);
urlstring.append(getDomain()).append("/overlap/id/").append(ids.get(0));
// @see https://github.com/Ensembl/ensembl-rest/wiki/Output-formats
urlstring.append("?content-type=text/x-gff3");
/*
* specify object_type=gene in case is shared by transcript and/or protein;
* currently only fetching features for gene sequences;
* refactor in future if needed to fetch for transcripts
*/
urlstring.append("&").append(OBJECT_TYPE).append("=")
.append(OBJECT_TYPE_GENE);
/*
* specify features to retrieve
* @see http://rest.ensembl.org/documentation/info/overlap_id
* could make the list a configurable entry in .jalview_properties
*/
for (EnsemblFeatureType feature : featuresWanted)
{
urlstring.append("&feature=").append(feature.name());
}
return new URL(urlstring.toString());
}
@Override
protected boolean useGetRequest()
{
return true;
}
/**
* Returns the MIME type for GFF3. For GET requests the Content-type header
* describes the required encoding of the response.
*/
@Override
protected String getRequestMimeType(boolean multipleIds)
{
return "text/x-gff3";
}
/**
* Returns the MIME type for GFF3.
*/
@Override
protected String getResponseMimeType()
{
return "text/x-gff3";
}
/**
* Overloaded method that allows a list of features to retrieve to be
* specified
*
* @param accId
* @param features
* @return
* @throws IOException
*/
protected AlignmentI getSequenceRecords(String accId,
EnsemblFeatureType[] features) throws IOException
{
featuresWanted = features;
return getSequenceRecords(accId);
}
}