package jalview.hmmer; import jalview.bin.Cache; import jalview.datamodel.Alignment; import jalview.datamodel.AlignmentAnnotation; import jalview.datamodel.AlignmentI; import jalview.datamodel.AnnotatedCollectionI; import jalview.datamodel.Annotation; import jalview.datamodel.HiddenMarkovModel; import jalview.datamodel.SequenceI; import jalview.gui.AlignFrame; import jalview.gui.JvOptionPane; import jalview.gui.Preferences; import jalview.io.HMMFile; import jalview.io.StockholmFile; import jalview.util.MessageManager; import java.io.BufferedReader; import java.io.File; import java.io.IOException; import java.io.InputStreamReader; import java.io.PrintWriter; import java.util.Hashtable; import java.util.List; /** * Base class for hmmbuild, hmmalign and hmmsearch * * @author TZVanaalten * */ public class HmmerCommand { public static final String HMMBUILD = "hmmbuild"; public String JALVIEWDIRECTORY = System.getProperty("user.dir") + File.separator; public String OUTPUTALIGNMENT; public final String SPACE = " "; public final String ALLCOL = "--allcol "; public final String TRIM = "--trim "; public final String FORCEAMINO = "--amino "; public final String FORCEDNA = "--dna "; public final String FORCERNA = "--rna "; Hashtable hash = new Hashtable(); List hmmSeqs; protected AlignFrame af; public static boolean isHmmerAvailable() { File exec = getExecutable(HMMBUILD, Cache.getProperty(Preferences.HMMER_PATH)); return exec != null; } /** * Uniquifies the sequences when exporting and stores their details in a * hashtable. * * @param seqs */ public void uniquifySequences(SequenceI[] seqs) { hash = jalview.analysis.SeqsetUtils.uniquify(seqs, true); } /** * Recover the sequence data lost by uniquifying. * * @param seqs */ public void recoverSequenceNames(SequenceI[] seqs) { jalview.analysis.SeqsetUtils.deuniquify(hash, seqs); } /** * Runs a command in the command line. * * @param command * @throws IOException * @throws InterruptedException */ public boolean runCommand(String command) throws IOException, InterruptedException { try { final Process p = Runtime.getRuntime().exec(command); new Thread(new Runnable() { @Override public void run() { BufferedReader input = new BufferedReader( new InputStreamReader(p.getInputStream())); String line = null; try { while ((line = input.readLine()) != null) { System.out.println(line); } } catch (IOException e) { e.printStackTrace(); } } }).start(); p.waitFor(); } catch (Exception e) { e.printStackTrace(); return false; } return true; } /** * Exports an alignment and/or HMM to the specified file. * * @param alignment * @throws IOException */ public void exportData(SequenceI[] seqs, File stoLocation, HiddenMarkovModel hmm, File hmmLocation, AnnotatedCollectionI al) throws IOException { if (seqs != null) { AlignmentI newAl = new Alignment(seqs); if (stoLocation != null && al != null) { for (AlignmentAnnotation annot : al.getAlignmentAnnotation()) { if (annot.label.contains("Reference") || "RF".equals(annot.label)) { AlignmentAnnotation newRF; if (annot.annotations.length > newAl.getWidth()) { Annotation[] rfAnnots = new Annotation[newAl.getWidth()]; System.arraycopy(annot.annotations, 0, rfAnnots, 0, rfAnnots.length); newRF = new AlignmentAnnotation("RF", "Reference Positions", rfAnnots); } else { newRF = new AlignmentAnnotation(annot); } newAl.addAnnotation(newRF); } } } StockholmFile file = new StockholmFile(newAl); String output = file.print(seqs, false); PrintWriter writer = new PrintWriter(stoLocation); writer.println(output); writer.close(); } if (hmm != null) { HMMFile file = new HMMFile(hmm); PrintWriter writer = new PrintWriter(hmmLocation); writer.print(file.print()); writer.close(); } } /** * Adds any HMM sequences removed before submitting the alignment as a job * back into the alignment. * * @param af */ public void addHMMConsensusSequences(AlignFrame af) { AlignmentI al = af.getViewport().getAlignment(); if (hmmSeqs == null || hmmSeqs.size() < 1) { return; } for (SequenceI seq : hmmSeqs) { Integer position = seq.getPreviousPosition(); al.getSequences().add(position, seq); } af.getViewport().setAlignment(al); af.alignPanel.adjustAnnotationHeight(); af.getViewport().updateSequenceIdColours(); af.buildSortByAnnotationScoresMenu(); } /** * Returns the list of HMM sequences removed * * @return */ public List getHmmSeqs() { return hmmSeqs; } /** * Sets the list of removed HMM sequences * * @param hmmSeqs */ public void setHmmSeqs(List hmmSeqs) { this.hmmSeqs = hmmSeqs; } /** * Answers the full path to the given hmmer executable, or null if file cannot * be found or is not executable * * @param cmd * command short name e.g. hmmalign * @return */ protected String getCommandRoot(String cmd) { String binariesFolder = Cache.getProperty(Preferences.HMMER_PATH); File file = getExecutable(cmd, binariesFolder); if (file == null && af != null) { JvOptionPane.showInternalMessageDialog(af, MessageManager.getString("warn.hmm_command_failed")); } return file == null ? null : file.getAbsolutePath(); } /** * Answers the executable file for the given hmmer command, or null if not * found or not executable. The path to the executable is the command name * prefixed by the hmmer binaries folder path, optionally with .exe appended. * * @param cmd * hmmer command short name, for example hmmbuild * @param binaryPath * parent folder containing hmmer executables * @return */ public static File getExecutable(String cmd, String binaryPath) { File file = new File(binaryPath, cmd); if (!file.canExecute()) { file = new File(binaryPath, cmd + ".exe"); { if (!file.canExecute()) { file = null; } } } return file; } }