JABAWS2 client August 2011 http://www.compbio.dundee.ac.uk/jabaws Usage: -h=host_and_context <-s=serviceName> ACTION [OPTIONS] -h= - a full URL to the JABAWS web server including context path e.g. http://10.31.1.159:8080/ws -s= - one of [MafftWS, MuscleWS, ClustalWS, ClustalOWS, TcoffeeWS, ProbconsWS, AAConWS, JronnWS, DisemblWS, GlobPlotWS, IUPredWS] is required for all ACTIONS but -list_services ACTIONS: -list_services - list available services -test - test service -i= - full path to fasta formatted sequence file, from which to align sequences -parameters - lists parameters supported by web service -presets - lists presets supported by web service -limits - lists web services limits Please note that if input file is specified other actions are ignored OPTIONS (only for use with -i action): -r= - name of the preset to use -o= - full path to the file where to write an alignment -f= - the name of the file with the list of parameters to use. Please note that -r and -f options cannot be used together. Alignment is done with either preset or a parameters from the file, but not both! EXAMPLES: 1) List all available services on the host Jws2Client -h=http://www.compbio.dundee.ac.uk/jabaws -list_services 2) Test Clustal web service Jws2Client -h=http://www.compbio.dundee.ac.uk/jabaws -s=ClustalWS -test 3) Align sequence from file input.txt with Probcons. Record resulting alignment into the output.txt Jws2Client -h=http://www.compbio.dundee.ac.uk/jabaws -s=ProbconsWS -i=input.txt -o=output.txt 4) Calculate disorder with Disembl take input from input.txt, output results to the console Jws2Client -h=http://www.compbio.dundee.ac.uk/jabaws -s=DisemblWS -i=input.txt 5) List all parameters available for AAconWS service Jws2Client -h=http://www.compbio.dundee.ac.uk/jabaws -s=AAconWS -parameters 6) Calculate conservation with AAConWS using LANDGRAF method, for Clustal alignment from input.txt and report the scores to the console Jws2Client -h=http://www.compbio.dundee.ac.uk/jabaws -s=AAconWS -i=input.txt -f=prm.txt Where the content of prm.txt file is -m=LANDGRAF The list of the supported parameters can be obtained as shown in the example 5. Citation: Peter V. Troshin, James B. Procter and Geoffrey J. Barton - "Java Bioinformatics Analysis Web Services for Multiple Sequence Alignment - JABAWS:MSA" Bioinformatics 2011; doi: 10.1093/bioinformatics/btr304.