X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=help%2Fhtml%2Fcalculations%2Fpca.html;fp=help%2Fhtml%2Fcalculations%2Fpca.html;h=ba9dc30605a082ef5eeccd059be55c6d5e9a1a83;hb=3f73b03257d72cbdd8cbe8889a6fc4fcab747dd3;hp=49019a7ec2541b44b465fb4090c8b1992ef49f04;hpb=1b9fc500a75a38d7c4408d4b209384d5236911cc;p=jalview.git diff --git a/help/html/calculations/pca.html b/help/html/calculations/pca.html index 49019a7..ba9dc30 100755 --- a/help/html/calculations/pca.html +++ b/help/html/calculations/pca.html @@ -32,7 +32,8 @@ for very large sets of sequences - usually because the JVM has run out of memory. A future release of Jalview will be able to avoid this by executing the calculation via a web service.
-About PCAPrincipal components analysis is a technique for examining the +
About PCA
+Principal components analysis is a technique for examining the structure of complex data sets. The components are a set of dimensions formed from the measured values in the data set, and the principle component is the one with the greatest magnitude, or length. The sets of @@ -52,26 +53,26 @@ of variation in the data set.
href="scorematrices.html#simplenucleotide">simple single nucleotide substitution matrix. The options available for calculation are given in the - Change Parameters menu.
+ PCA Calculation modes
+ The default Jalview calculation mode
+ (indicated when Jalview PCA Calculation is
+ ticked in the Change Parameters menu) is to
+ perform a PCA on a matrix where elements in the upper diagonal give
+ the sum of scores for mutating in one direction, and the lower
+ diagonal is the sum of scores for mutating in the other. For protein
+ substitution models like BLOSUM62, this gives an asymmetric matrix,
+ and a different PCA to a matrix produced with the method described in the
+ paper by G. Casari, C. Sander and A. Valencia. Structural Biology
+ volume 2, no. 2, February 1995 (pubmed)
- and implemented at the SeqSpace server at the EBI. The original method
- preconditions the matrix by multiplying it with its transpose, and
- this mode is enabled by unchecking the
- Jalview PCA Calculation option in the
- Change Parameters menu.
+ and implemented at the SeqSpace server at the EBI. This method
+ preconditions the matrix by multiplying it with its transpose, and can be employed in the PCA viewer by unchecking the Jalview
+ PCA Calculation option in the Change
+ Parameters menu.
The PCA Viewer
This is an interactive display of the sequences positioned within the similarity space, as points in a rotateable 3D scatterplot. The