X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=src%2Fjalview%2Fext%2Fensembl%2FEnsemblCdna.java;h=738432708c4cd3917e9b2abe8971f9f122079d97;hb=a4f2a7f356b8edab17a9a5bb6f2e71a1419792a9;hp=a2ecfcdf101ab0456ff9824470f5622f0fa5cebf;hpb=b4fac54ddfb91688f281a6a2ede0d8d44ec1dd13;p=jalview.git diff --git a/src/jalview/ext/ensembl/EnsemblCdna.java b/src/jalview/ext/ensembl/EnsemblCdna.java index a2ecfcd..7384327 100644 --- a/src/jalview/ext/ensembl/EnsemblCdna.java +++ b/src/jalview/ext/ensembl/EnsemblCdna.java @@ -1,25 +1,79 @@ +/* + * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$) + * Copyright (C) $$Year-Rel$$ The Jalview Authors + * + * This file is part of Jalview. + * + * Jalview is free software: you can redistribute it and/or + * modify it under the terms of the GNU General Public License + * as published by the Free Software Foundation, either version 3 + * of the License, or (at your option) any later version. + * + * Jalview is distributed in the hope that it will be useful, but + * WITHOUT ANY WARRANTY; without even the implied warranty + * of MERCHANTABILITY or FITNESS FOR A PARTICULAR + * PURPOSE. See the GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with Jalview. If not, see . + * The Jalview Authors are detailed in the 'AUTHORS' file. + */ package jalview.ext.ensembl; import jalview.datamodel.SequenceFeature; -import jalview.io.gff.SequenceOntology; +import jalview.datamodel.SequenceI; +import jalview.io.gff.SequenceOntologyFactory; +import jalview.io.gff.SequenceOntologyI; + +import java.util.ArrayList; +import java.util.List; import com.stevesoft.pat.Regex; +/** + * A client to fetch CDNA sequence from Ensembl (i.e. that part of the genomic + * sequence that is transcribed to RNA, but not necessarily translated to + * protein) + * + * @author gmcarstairs + * + */ public class EnsemblCdna extends EnsemblSeqProxy { /* - * fetch exon features on genomic sequence (to identify the cdnaregions) + * accepts ENST or ENSTG with 11 digits + * or ENSMUST or similar for other species + * or CCDSnnnnn.nn with at least 3 digits + */ + private static final Regex ACCESSION_REGEX = new Regex( + "(ENS([A-Z]{3}|)[TG][0-9]{11}$)" + "|" + "(CCDS[0-9.]{3,}$)"); + + /* + * fetch exon features on genomic sequence (to identify the cdna regions) * and cds and variation features (to retain) */ private static final EnsemblFeatureType[] FEATURES_TO_FETCH = { EnsemblFeatureType.exon, EnsemblFeatureType.cds, EnsemblFeatureType.variation }; + /** + * Default constructor (to use rest.ensembl.org) + */ public EnsemblCdna() { super(); } + /** + * Constructor given the target domain to fetch data from + * + * @param d + */ + public EnsemblCdna(String d) + { + super(d); + } + @Override public String getDbName() { @@ -35,7 +89,7 @@ public class EnsemblCdna extends EnsemblSeqProxy @Override public Regex getAccessionValidator() { - return new Regex("((ENST|ENSG|CCDS)[0-9.]{3,})"); + return ACCESSION_REGEX; } @Override @@ -59,23 +113,37 @@ public class EnsemblCdna extends EnsemblSeqProxy } /** - * Answers true if the sequence feature type is 'exon' (or a subtype of exon - * in the Sequence Ontology), and the Parent of the feature is the transcript - * we are retrieving + * Answers a list of sequence features (if any) whose type is 'exon' (or a + * subtype of exon in the Sequence Ontology), and whose Parent is the + * transcript we are retrieving */ @Override - protected boolean identifiesSequence(SequenceFeature sf, String accId) + protected List getIdentifyingFeatures(SequenceI seq, + String accId) { - if (SequenceOntology.getInstance().isA(sf.getType(), - SequenceOntology.EXON)) + List result = new ArrayList<>(); + List sfs = seq.getFeatures() + .getFeaturesByOntology(SequenceOntologyI.EXON); + for (SequenceFeature sf : sfs) { String parentFeature = (String) sf.getValue(PARENT); if (("transcript:" + accId).equals(parentFeature)) { - return true; + result.add(sf); } } - return false; + + return result; + } + + /** + * Parameter object_type=Transcaript added to ensure cdna and not peptide is + * returned (JAL-2529) + */ + @Override + protected String getObjectType() + { + return OBJECT_TYPE_TRANSCRIPT; } }