# Illustration of ALSCRIPT Command File # # Illustrates some of the MASK commands # # example 1 # Comment lines begin with the # symbol # # # (A) Input and output files: # BLOCK_FILE example1.blc # Define the block file to format OUTPUT_FILE example4.ps # Where to put the result # MAX_SIDE 11 # Fiddle with margins... Y_OFFSET 50 # This is just to fill the page with the X_OFFSET 80 # Alignment. Defaults normally work OK # # (B) Page Layout and overall spacing: # ADD_SEQ 38 1 # Make space after sequence 38 ADD_SEQ 39 3 # Make space after sequence 39 ADD_SEQ 69 1 PORTRAIT # Portrait paper orientation POINTSIZE 10 # 10 point default pointsize #NUMBER_SEQS 1 # Set to 1 for numbers at left of plot IDENT_WIDTH 8 # # (C) Font definitions: # DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica DEFINE_FONT 3 Helvetica-Bold DEFAULT DEFINE_FONT 4 Times-Bold DEFAULT DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT DEFINE_FONT 6 Times-Roman DEFAULT SETUP # Tell the program to get on with the formatting # # some formatting commands # SURROUND_CHARS LIV ALL # Surround all L and Y characters FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps FONT_CHARS KR ALL 4 # Show K and R in Times-Bold BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 SHADE_CHARS + ALL 0.0 # Shade all + characters black SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 LINE TOP 2 40 8 # Draw horizontal lines to LINE BOTTOM 2 40 8 # Join up surrounded numbers TEXT 2 42 "Helix Pattern" TEXT 12 42 "Glycine Loop" # Add text annotations TEXT 21 42 "Helix Pattern" SUB_ID 49 "Helix Predictions" SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms SUB_ID 68 "Turn Predictions" #INVERSE_CHARS DE ALL #SHADE_CHARS D ALL 0.5 #SHADE_CHARS E ALL 0.0 # # (D) Some formatting commands: to illustrate the use of MASK # mask SETUP #setup the mask sub_chars 1 1 27 38 SPACE . #substitute spaces for dots mask ILLEGAL "." #. is not to be used in frequency calculation mask FRE 1 1 27 38 # do the frequency calculation mask BOX 1 1 27 38 # use the resulting mask to create boxing lines mask SHADE 1 1 27 38 0.2 # shade mask INVERSE 1 1 27 38 # inverse - could also alter the font sub_chars 1 1 27 38 . SPACE # switch the dots back to spaces # # (E) Now make the prediction histograms nicer. # SURROUND_CHARS H 1 44 27 53 SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" # SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions # SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions # SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction SHADE_CHARS HY 1 75 27 75 0.5 SHADE_CHARS T 1 75 27 75 0.9 # # # Now change the identifier codes # ID_FONT ALL 6 SUB_ID 1 "Annexin I H4" SUB_ID 2 "Annexin I M4" SUB_ID 3 "Annexin I R4" SUB_ID 4 "Annexin V R4" SUB_ID 5 "Annexin V H4" SUB_ID 6 "Annexin V C4" SUB_ID 7 "Annexin II H4" SUB_ID 8 "Annexin II B4" SUB_ID 9 "Annexin II M4" SUB_ID 10 "Annexin IV P4" SUB_ID 11 "Annexin IV B4" SUB_ID 12 "Annexin IV H4" SUB_ID 13 "Annexin VI H4" SUB_ID 14 "Annexin VI M4" SUB_ID 15 "Annexin VI H8" SUB_ID 16 "Annexin VI M8" SUB_ID 17 "Annexin V BH4" SUB_ID 18 "Annexin VII 4" SUB_ID 19 "Annexin III H4" SUB_ID 20 "Annexin III R4" SUB_ID 21 "Annexin II M3" SUB_ID 22 "Annexin II H3" SUB_ID 23 "Annexin II B3" SUB_ID 24 "Annexin I H3" SUB_ID 25 "Annexin I M3" SUB_ID 26 "Annexin I R3" SUB_ID 27 "Annexin V C3" SUB_ID 28 "Annexin V H3" SUB_ID 29 "Annexin V R3" SUB_ID 30 "Annexin VI H3" SUB_ID 31 "Annexin VI M3" SUB_ID 32 "Annexin IV H3" SUB_ID 33 "Annexin IV B3" SUB_ID 34 "Annexin IV P3" SUB_ID 35 "Annexin V BH3" SUB_ID 36 "Annexin III H3" SUB_ID 37 "Annexin III R3" SUB_ID 38 "Annexin VII 3"