--- /dev/null
+SOFTWARE LICENCE AGREEMENT FOR ACADEMIC USE OF ALSCRIPT\r
+\r
+IMPORTANT: This software and its associated documentation are the copyright\r
+works of the author, Geoffrey J. Barton hereinafter referred to as the\r
+LICENSOR. Use of the software and documentation is governed by the terms of\r
+the Academic License Agreement set out below. You, hereinafter referred to\r
+as the LICENSEE, will not be able to install the software unless you first\r
+agree to those terms.\r
+\r
+The LICENSOR has developed a body of computer software and associated\r
+documentation called ALSCRIPT hereafter referred to as the WORK.\r
+\r
+The LICENSEE desires to use the WORK for education and research purposes.\r
+\r
+The LICENSEE and LICENSOR agree as follows:\r
+\r
+The LICENSOR grants to the LICENSEE a nonexclusive, nontransferable,\r
+licence to use the WORK subject to the following conditions.\r
+\r
+1. LICENSEE'S RIGHTS\r
+\r
+The Licensee shall have the right to use the WORK for educational and\r
+research purposes on all computers owned or leased by the Licensee and\r
+located on the LICENSEE'S campus or site.\r
+\r
+2. RESTRICTIONS ON USE\r
+\r
+No commercial use of any kind is permitted under this licence. For\r
+commercial use, a commercial licence is required.\r
+\r
+3. LICENSING FEE\r
+\r
+For a distribution via electronic means, there is no fee. For a physical\r
+distribution on magnetic media, the fee is fifty (50) pounds (UK).\r
+\r
+4. NO SUPPORT\r
+\r
+The LICENSEE recognizes that the LICENSOR is not obligated to provide\r
+support, maintenance, consulting, or revision of the WORK. If the LICENSOR\r
+chooses to release to the LICENSEE updates of, additions to, or\r
+modifications of the WORK, this agreement shall apply to them as though\r
+they were part of the original WORK.\r
+\r
+5. NO PRODUCT WARRANTY\r
+\r
+The WORK is released on a "as is" basis. There is no warranty whatsoever as\r
+to functioning, performance or effect on hardware or other software,\r
+express or implied. The LICENSOR disclaims any implied warranties of\r
+merchantability or fitness for any particular purpose.\r
+\r
+6. OWNERSHIP\r
+\r
+The LICENSEE agrees that the WORK including any updates, additions, and\r
+modifications, is, and shall at all times remain, the property of the\r
+LICENSOR, and that it has been copyrighted by the LICENSOR. The LICENSEE\r
+shall have no right, title or interest therein or thereto except as\r
+expressly set forth in this agreement.\r
+\r
+7. CREDITS\r
+\r
+All credits and copyright notices in the WORK, both in listings and/or\r
+documentation, whether names of individuals or organisations, shall be\r
+retained in place. Publications referring to the WORK, or to other works\r
+containing the WORK in whole or in part, shall refer to it as ALSCRIPT and\r
+shall specify that the WORK was made by Geoffrey J. Barton. Publication of\r
+results that use the WORK shall cite: Barton, G. J. (1993), Prot. Eng., 6,\r
+37-40.\r
+\r
+8. NONDISCLOSURE\r
+\r
+Under no conditions shall the LICENSEE disclose the WORK, in whole or in\r
+part, to third parties, except as expressly provided for in this agreement.\r
+Nor shall the LICENSEE make the WORK available to third parties via a\r
+computer network. Permission is hereby granted to the LICENSEE to disclose\r
+the WORK or modifications thereof to other organisations in possession of a\r
+valid source licence for the WORK, provided that such disclosure shall be\r
+for educational or research purposes only. LICENSEE may also disclose the\r
+WORK to its students and employees for use in their educational and\r
+research activities, provided that they are bound not to further disclose\r
+it to third parties. This article shall survive termination of the\r
+agreement.\r
+\r
+9. NO LIABILITY\r
+\r
+Neither the LICENSOR nor any individual or any legal entity involved in\r
+creating, modifying, updating, or supplementing the work, shall be liable\r
+for damages arising out of the failure or malfunctioning of the WORK. The\r
+LICENSEE hereby assumes the risk of and releases and forever discharges the\r
+LICENSOR, its employees and any other individual or legal entity referred\r
+to in the foregoing sentence with respect to any expense, claim, liability,\r
+loss or damage, direct or indirect, including any incidental or\r
+consequential damages, whether made or suffered by LICENSEE in connection\r
+with the failure or malfunction of the WORK. LICENSEE acknowledges that the\r
+WORK is in the process of development and is not error-free, that the\r
+foregoing exclusion of liability is therefore an essential term of this\r
+Agreement without which exclusion the LICENSOR would not be willing to\r
+enter into this Agreement and to make the Work available on the Price\r
+agreed upon herein.\r
+\r
+10. GOVERNING LAW\r
+\r
+This agreement shall be construed and enforced according to the laws of\r
+England.\r
+\r
+11. TERMINATION AND ENFORCEMENT COSTS\r
+\r
+LICENSOR shall have the right to terminate this agreement with immediate\r
+effect upon notice by registered mail to LICENSEE in the event that\r
+LICENSEE, its employees, or persons acting on its behalf breach any\r
+provision of this agreement. Upon termination, the LICENSEE agrees to\r
+return the orginal WORK immediately, to destroy all copies of the WORK\r
+(exact or modified) in its possession or under its control, and to send to\r
+the LICENSOR a signed statement that all such copies have been destroyed.\r
+If the LICENSOR takes legal action against the LICENSEE to enforce this\r
+agreement and prevails, the LICENSEE agrees to pay LICENSOR'S legal costs,\r
+including reasonable attorney's fees.\r
+\r
+12. CHANGES TO THIS AGREEMENT\r
+\r
+This agreement may only be changed if both parties agree to the proposed\r
+changes in writing.\r
--- /dev/null
+
+
+This file contains details of the terms of use for alscript.
+
+Please see the file LICENSE for details of the Academic Licence for ALSCRIPT.
+
--- /dev/null
+
+
+
+Running the alscript suite under Microsoft Windows 95/NT and Win32.
+
+In version 2.07 there is directory called:
+
+bin/cygwin
+
+This includes executables and a .dll file compiled to run on microsoft windows.
+If you add this directory to your path, or execute alscript from this directory,
+you will be able to run alscript under windows.
+
+This new feature uses the cygwin environment, for further details see:
+
+http://www.cygnus.com
+
+GJB 9/July/1999
+
+
--- /dev/null
+
+Version 2.07 - 9 July 1999
+
+Minor changes to makefiles to allow compilation of all modules under Linux. Addition
+of cygwin .dll file to allow the code to run under windows.
+
+Please see the README_WINDOWS file.
+
+Version 2.06
+
+Includes options by Morten Kjeldgaard <mok@imsb.au.dk>
+
+small arrow up filled/hollow (@fsuparrow/@suparrow)
+small arrow down filled/hollow (@fsdownarrow/@sdownarrow)
+diamond filled/hollow (@fdiamond/@diamond)
+bar filled/hollow (@fbar/@bar)
+
+Thanks to Morten for making these additions available.
+
+
+
--- /dev/null
+#!/bin/csh
+# csh script to compile the elements of alscript
+#
+# type ./build <machine>
+#
+# e.g. ./build sun or ./build sgi or ./build gcc
+#
+# ./build linux
+#
+# Note that BUILD linux should also work under cygwin, though
+# you will need to add --unix to the make arguments.
+# In release 2.07 the cygwin binaries are included in the distribution.
+#
+# This script will create a subdirectory /bin if it does not
+# already exist. It will then create a subsub directory /bin/machine
+# where executables for the architecture will be stored.
+#
+# You should then add this directory to your path, or add links
+# from a directory that is on your path (e.g. /usr/local/bin)
+#
+echo ""
+echo "Trying to build alscript for $1"
+echo ""
+if(! -e bin) then
+ echo "Creating bin directory"
+ mkdir bin
+endif
+if(! -e bin/$1) then
+ echo "Creating subdirectory bin/$1"
+ mkdir bin/$1
+endif
+if(-e src/alscript.o) then
+ echo "Deleting old object files"
+ /bin/rm src/*.o
+endif
+echo "Copying Makefile"
+/bin/cp src/makefile.$1 src/makefile
+echo "Attempting make"
+make -C src
+echo ""
+echo "Moving executables to bin directory"
+echo ""
+/bin/mv src/alscript bin/$1
+/bin/mv src/msf2blc bin/$1
+/bin/mv src/clus2blc bin/$1
+/bin/mv src/fast2blc bin/$1
+/bin/mv src/alsnum bin/$1
+echo ""
+echo "Deleting object files"
+echo ""
+/bin/rm src/*.o gjutil/*.o
+echo ""
+echo " All should be complete!"
+echo ""
--- /dev/null
+This directory contains documentation for ALSCRIPT.
+
+alscript.tex - LaTeX document should be readable in any text editor.
+alscript.ps - PostScript document - should be printable on a PostScript
+ printer. It is formatted for US Letter paper.
+
+alscript/alscript.html - html version of documentation.
+
+
--- /dev/null
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+%%Trailer
+end
+userdict /end-hook known{end-hook}if
+%%EOF
--- /dev/null
+\documentstyle[12pt,times,html]{article}
+\begin{document}
+\parindent 0in
+%\raggedright
+\bibliographystyle{jmb}
+\nocite{TitlesOn}
+\begin{titlepage}
+%\begin{singlespace}
+\begin{center}
+\begin{Huge}
+\begin{bf}
+\vskip 0.5in
+
+User Guide to
+
+ALSCRIPT - Sequence alignment to PostScript\\
+
+Version 2.03\\
+
+\end{bf}
+\end{Huge}
+
+\vskip 0.5in
+
+\begin{large}
+{\em Geoffrey J. Barton}
+\vskip 0.5in
+
+
+Laboratory of Molecular Biophysics\\
+University of Oxford\\
+Rex Richards Building\\
+South Parks Road\\
+Oxford OX1 3QU\\
+U.K.\\
+
+\vskip 0.25in
+
+\end{large}
+\vskip 0.25in
+Tel: (44) 1865-275368\\
+Fax: (44) 1865-510454\\
+e-mail: gjb@bioch.ox.ac.uk
+
+\vskip 0.25in
+
+REFERENCE:\\
+ Barton, G. J. (1993),\\
+ ALSCRIPT a tool to format multiple sequence alignments\\
+ Protein Engineering, Volume 6, No. 1, pp.37-40.\\
+\vskip 0.25in
+
+\end{center}
+\end{titlepage}
+\tableofcontents
+
+\section{Update History}
+\begin{verbatim}
+
+VERSION 1.0 19 June 1992
+Version 1.1 26 June 1992
+Version 1.2 21 October 1992: Add multiple blocks per page option.
+Version 1.3 15 November 1992: First Distribution.
+Version 1.4 6 December 1992: Add Colour commands.
+Version 1.4.1 1 February 1993: Small bug fixes - FULL RELEASE VERSION.
+Version 1.4.2 15 February 1993: Make silent_mode toggle.
+Version 1.4.3 1 March 1993: Fix bug in colour option.
+Version 1.4.4 24 March 1993: Add mask features (should be version 1.5).
+Version 1.4.5 25 May 1993: Include alsnum program in distribution.
+ 7 June 1993: Fix NO_NUMBERS bug in documentation
+ Change defaults for -q option to use MASK.
+
+Version 2.0 23 May 1995: Numerous changes and additions including the
+ option to colour backgrounds
+ differently, ommission of idents on
+ second and subsequent lines, helix,
+ strand and other special characters,
+ relative numbering, error checking of
+ ranges on input, bounding box,
+ screening, conservation colouring...
+Version 2.03 5 June 1996: Small bug fixes - patches incorporated.
+ BACKGROUND_REGION and BOUNDING_BOX commands moved to
+ step 1 section.
+
+\end{verbatim}
+
+\section{Read This First - VERSION 2.0}
+
+This manual describes an interim release of ALSCRIPT that includes
+many additional features over the previously distributed Version
+1.4.5. I had hoped to make a lot more changes and improvements before
+distributing the new version, however I have not had the time to do
+this. I am distributing Version 2.0 since the new features have been
+used in a number of published alignment figures. Please see
+\hyperref{New Features in Version 2.0}{see the Section }{ }{new2} for details
+of the new features.
+
+\section{Related Programs}
+
+The AMPS package (Barton, 1990). This performs multiple sequence
+alignments and databank scanning.
+
+AMAS (Livingstone and Barton, 1993, CABIOS, 9, 745-756). Analysis of
+Multiply Aligned Sequences. This package uses a sophisticated
+set-based method to identify patterns of residue conservation in
+multiple sequence alignments.
+
+All programs are available by anonymous ftp from geoff.biop.ox.ac.uk.
+Please see the README file for details licencing and registration.
+You can read manuals for the programs and some related papers at
+http://geoff.biop.ox.ac.uk/.
+
+\section{Availability}
+
+ALSCRIPT is available free of charge for academic and non-commercial
+purposes. Distribution is by anonymous ftp from geoff.biop.ox.ac.uk.
+See the README file on the ftp server for details. You need to
+register with G. J. Barton before downloading the software.
+
+\section{Installing ALSCRIPT}
+
+See the appropriate section for the computer type you are using:
+
+For PCs see \hyperref{386 MS-DOS installation}{see Section }{}{app4}.
+For Unix see \hyperref{Unix installation}{see Section }{ }{app5}.
+For VMS see \hyperref{VMS installation}{see Section }{ }{app6}.
+
+\section{Brief Description of ALSCRIPT}
+
+ALSCRIPT takes a multiple sequence alignment in AMPS (Barton \&
+Sternberg, 1987, Barton, 1990) block-file format and a set of
+formatting commands and produces a PostScript file that may be printed
+on a PostScript laser printer, or viewed using a PostScript previewer
+(e.g. Sun Microsystem's PageView program). CLUSTAL and GCG format
+multiple alignment files may also be used (see below). ALSCRIPT is NOT a
+multiple sequence alignment program, nor is it an alignment editor.
+
+Given a block-file and pointsize (character width/height), ALSCRIPT
+calculates how many residues can be fitted across the page, and how
+many sequences will fit down the page, it then prints the alignment at
+the chosen pointsize on as many pages as are needed. Running ALSCRIPT with
+a smaller or larger pointsize will automatically re-scale the alignment
+to fit on fewer or more pages as appropriate. The actual page
+dimensions may be re-set to any value, so if you have access to an A3
+PostScript printer, or typesetting machine, alignments can readily be
+scaled to maximise the available space.
+
+Each output page has three basic regions. The left hand edge contains
+identifier codes for each sequence. The main part of the page holds the
+alignment, and the top part, the position numbers and tick marks. ALSCRIPT
+commands make use of a character coordinate system for font changes,
+and other formatting commands. Thus, any residue in the alignment may
+be referred to by its sequence position number (x-axis) and sequence
+number (y-axis), similarly, ranges of residue positions, or sequences
+may also be defined in the character coordinate system.
+
+The basic ALSCRIPT commands allow the following functionality:
+
+Fonts: Any PostScript font at any size may be defined and used on
+individual residues, regions or identifier codes.
+
+Boxing: Simple rectangular boxes may be drawn around any part of the
+alignment. Particular residue types may be selected and automatically
+"surrounded" by lines. For example, if the characters 'G' and 'P' are
+selected, then lines will not be drawn between G and P characters, but
+only where G and P border with other characters.
+
+Shading: Grey shading of any level from black to white may be applied
+to any region of the alignment, either as a rectangular region, or as
+residue specific shading. e.g. "shade all Cys residues between
+positions 6 and 30"
+
+Text: Specific text strings may be added to the alignment at any
+position and in any font or font size.
+
+Lines: Horizontal or vertical lines may be drawn to the left, right, top
+or bottom of any residue position or group of positions.
+
+Colour: Characters or character backgrounds may be independently
+coloured.
+
+The example block file "example1.blc" and command file "example1.als"
+illustrate most of these commands in action.
+
+Although written with the aim of producing figures for journal
+submission, ALSCRIPT may be used as a tool for interpreting multiple
+sequence alignments. For example, the boxing, shading and font changing
+facilities can be applied to highlight amino acids of a particular type
+and thus draw attention to clusters of positive or negative charge,
+hydrophobics, etc.
+
+
+\section{New Features in Version 1.4.4}
+
+This version introduces the MASK family of commands which allows
+boxing, shading etc to be applied according to the frequency of
+occurence of the character types at each position in the alignment.
+For example, it is possible to box positions where one character is
+seen in more than N of the sequences. It is also possible to
+box/shade etc the most frequently occurring character at each
+position. Commands exist to select which characters will be used in
+the calculation of frequencies and which will be excluded, thus boxing
+can be based upon two or more character types at a position. MASK
+commands also exist to show residues identical to one sequence in the
+set. See the section on MASK below for details.
+
+NOTE: Although boxing according to the frequency of amino acids seen
+at a position is a popular method of representation it is not usually
+the most informative. An analysis that takes into account the
+physico-chemical properties of the amino acids and also relates the
+amino acid similarities to the overall similarity between the
+sequences is more helpful in identifying functionally important
+residues. The AMAS program (Livingstone and Barton, 1993) applies a
+flexible hierarchical set-based approach to this problem.
+
+\section{New Features in Version 1.4.5 - Program alsnum}
+
+Version 1.4.5 includes the program "alsnum". This is a temporary solution to
+the residue numbering problem. Ultimately, these functions will be included
+as alscript commands.
+
+alsnum creates a set of TEXT commands that can be incorporated into an
+alscript command file to place sequential numbers at any position in the
+alignment. The numbers ignore gaps, so the numbering will correspond to
+the specific sequence position rather than the alignment.
+
+To use the program:
+
+1. Decide where you want the numbers to be placed. For example, you might
+want the numbers above the third sequence in the alignment. If so, make
+an extra sequence space above the third sequence using the ADD\_SEQ\ command.
+
+2. Decide what is the number of the first residue of the sequence to be
+numbered. This will not always be 1 since you may be aligning fragments
+or domains.
+
+3. Decide the numbering interval (e.g. every 10th amino acid).
+
+4. Run the program.
+
+For example, if you want to add numbers according to sequence 37 of a
+block file (junk.blc), calling the first residue of the sequence 12,
+and with an interval of 5, and the numbers are to be placed at the
+location of sequence 3 in the alignment. Type:
+
+alsnum 37 12 5 3 $<$ junk.blc $>$ junk.text
+
+5. Add the resulting TEXT commands from junk.text to your alscript
+command file.
+
+\section{New Features in Version 2.0}
+\label{new2}
+
+Error checking is now done on all ranges input. If you run ALSCRIPT
+2.0 on a file that worked with ALSCRIPT 1.4.5, and it complains about
+out of range numbers, then check your ranges carefully. If you think
+you are right, then send me a minimal example of the problem and I
+will investigate. Versions of ALSCRIPT before 2.0 would often work
+happily with out of range numbers and produce perfectly OK output.
+
+The files {\bf ipns.als} and {\bf ipns.blc} show example command and
+block file that use most of these new commands. See the {\em
+examples} directory.
+
+\subsection{New Step 1 Commands}
+
+SCREENSIZE 120
+
+Usually you should not need to change this value, it alters the
+screening used by the printer. A value of 120 is used by default. On
+most 300dpi black and white printers this gives much smoother greys
+than the default used in earlier versions of ALSCRIPT.
+
+PIR\_SAVE\ filename
+
+Will cause the block file to be saved into the file ``filename'' in
+PIR format. This can be useful for moving block file alignments to other
+programs.
+
+MSF\_SAVE\ filename
+
+Will cause the block file to be saved into the file ``filename'' in
+something that approximates GCG .msf format. {\em Warning! This has not
+been fully tested.}
+
+NUMBER\_COLOUR\ 4
+
+Sets the colour used for numbering at the top of the alignment (no
+American spelling at the moment). In this example, colour number 4 has been
+defined (See the DEFINE\_COLOUR command if you are not sure what this means).
+
+SINGLE\_PAGE
+
+If this is set, ALSCRIPT assumes everything will be plotted on one
+page. At the moment, all this does is write the bounding box for the
+figure, so encapsulating the PostScript. This {\em may} allow the
+output of alscript to be imported into word processors etc, but
+probably not all.
+
+ID\_ONLY\_ON\_FIRST\_LINE
+
+If this is present, then sequence identifiers will only be printed on
+the first line of the alignment. Often this looks better for small
+alignments than the default.
+
+BACKGROUND\_COLOUR\ 7
+
+Sets the colour used for the background to the alignment. This can be
+useful for preparing figures for projection. At the moment this only
+works reliably when the SINGLE\_PAGE is also set.
+
+
+BOUNDING\_BOX\ x\ y\ x1\ y1
+
+Defines the bounding box for the figure. This is set in points (1/72 inch).
+NOTE: In version 2.0 this was a STEP 2 Command.
+
+BACKGROUND\_REGION\ x\ y\ x1\ y1
+
+Defines the region to colour as background - the default is set up for A4 paper
+so US users may have to fiddle with this. Values are points (1/72 inch).
+NOTE: In version 2.0 this was a STEP 2 Command.
+
+\subsection{New Step 2 Commands}
+
+
+COLOUR\_TEXT\_REGION\ x\ y\ x1\ y1 colour
+
+Sets the colour for TEXT command output. Similar syntax to COLOUR\_REGION, FONT\_REGION etc.
+
+COLOUR\_LINE\_REGION\ x\ y\ x1\ y1 colour
+
+Set the colour for LINEs in a region.
+
+CALCONS\ x\ y\ x1\ y1
+
+Calculate conservation values according to Zvelebil {\em et al.} for the designated
+region. (See Livingstone \& Barton 1993 for details and further refs)
+
+MASK\ CONSERVATION\ cutoff
+
+If CONSCAL has been used, then mask residues according to the conservation cutoff.
+
+e.g. MASK CONSERVATION 10 would mask all identities, MASK CONSERVATION 6 would
+mask reasonably conserved positions. See examples for more on this command.
+
+HELIX\ x1\ y\ x2
+
+Draw a helix from x1 to x2 of sequence y.
+
+STRAND\ x1\ y\ x2
+
+Draw a strand from x1 to x2 of sequence y.
+
+COIL\ x1\ y\ x2
+
+Draw a coil (horizontal line) from x1 to x2 of sequence y.
+
+RELATIVE\_TO <seqnum> <startnum>
+
+Set reference numbers to work relative to sequence number <seqnum>. This
+means that in all subsequent commands, ALSCRIPT will translate your x
+values into absolute position values in the alignment. This is {\em
+extremely} useful since you can annotate your alignment using your
+favourite sequences as a reference point. You no longer have to
+translate every x position into the alignment position.
+
+<startnum> is optional. If present, it specifies what the first
+residue in the displayed sequence is. For example, you may be showing
+residues 200-500 of a sequence, so <startnum> would be 200 rather than
+the default of 1. {\em Warning - this is a very new feature and
+bounds checking is not fully enabled for it}.
+
+You can use RELATIVE\_TO several times in the command file to annotate
+different sequences. RELATIVE\_TO 0 resets to the ``normal''
+alignment numbering.
+
+\subsection{New special TEXT commands}
+
+Some special TEXT commands have been added to allow drawing of alternative
+shapes etc. In fact this is how the HELIX, STRAND and COIL commands are implemented. The text commands are all prefixed by an @ symbol.
+
+e.g. TEXT 3 6 ``@fuparrow''
+
+will draw a filled up arrow at position 3,6.
+
+The alternative text commands are:
+
+@leftarrow - an open left pointing arrow.
+
+@fleftarrow - a filled left pointing arrow.
+
+@uparrow - an open up pointing arrow.
+
+@fuparrow - a filled up pointing arrow.
+
+@downarrow - an open down pointing arrow.
+
+@fdownarrow - a filled down pointing arrow.
+
+@circle - an open circle.
+
+@fcircle - a filled circle.
+
+I plan to make this option more flexible in the near future.
+
+\section{Running ALSCRIPT}
+\subsection{Basic Use}
+
+I recommend you read through this section, then scan the commands in
+\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1} to get a feel for what ALSCRIPT can do.
+
+See \hyperref{Alternative ways of invoking ALSCRIPT}{Section }{for
+alternative methods of invoking ALSCRIPT}{app7}. In this section, the
+interactive method is described. The QUICK START method shown in
+\hyperref{alternative ways of invoking ALSCRIPT}{Section }{ }{app7}
+is useful to format a sequence alignment quickly
+using standard pointsize and shading.
+
+ALSCRIPT is designed to work with AMPS block file format multiple
+alignments. If you have a multiple alignment generated by CLUSTAL V
+or the GCG package, then it must be translated to AMPS block file
+format.
+
+To translate a GCG .MSF file: Type: msf2blc.
+To translate a CLUSTAL PIR format file, or any PIR format file: clus2blc.
+
+Both programs prompt for the name of an input file, and an output
+block file name. A good convention to follow is to name all blockfiles
+with the extension ".blc".
+
+To run ALSCRIPT simply type:
+
+alscript
+
+you will then be prompted for the name of the ALSCRIPT command file.
+Having typed the filename, the commands will be executed as you have
+specified.
+
+A Simple Command File (example.als)
+
+The file example.blc contains a small multiple sequence alignment.
+The following ALSCRIPT command file will convert this into a
+PostScript alignment file in 12 point Helvetica.
+
+\begin{verbatim}
+#Comments in ALSCRIPT command files start with a #
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+SETUP #Tell the program to get on with it.
+\end{verbatim}
+
+Now try changing the POINTSIZE value to 5 ALSCRIPT will re-format the
+alignment to make best use of the available paper.
+
+These are all STEP 1 commands - they refer to overall layout, and system
+settings - for example, the paper size or maximum sequence length.
+Other commonly used STEP 1 commands are IDENT\_WIDTH\ which reserves more
+or less width for the sequence identifier codes, NUMBER\_SEQS\ which adds
+a number to each sequence and LINE\_WIDTH\_FACTOR\ which allows the
+thickness of all boxing lines to be adjusted. See
+\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1}
+for details of these and all other STEP 1 commands.
+
+The simple example outlined above can be modified with a variety of
+STEP 2 commands.
+
+for example file example2.als:
+
+\begin{verbatim}
+# FILE example2.als
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example2.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica
+DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic
+NUMBER_SEQS #Number the sequences at left hand side
+SETUP #Tell the program to get on with it.
+#
+#step 2 commands come after the SETUP command
+#
+#Here are some examples...
+#
+SURROUND_CHARS GP ALL #draw lines around all G and P
+SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6
+BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20
+FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters
+ID_FONT ALL 1 #set identifiers in font 1
+
+\end{verbatim}
+
+There are many possible ways of combining these commands and the
+others shown in
+\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1}.
+In general, if you apply multiple
+commands to the same residue, the effect of the last applied command
+persists where there would otherwise be a conflict. Thus the
+intersection of two overlapping SHADed regions would be shaded
+according to the second SHADE command, not some mixture of the two.
+Similarly for FONT commands. BOX and SURROUND commands behave in the
+opposite sense, all BOXing and SURROUNDing persists regardless of how
+many commands you issue. This makes it possible for example, to
+SURROUND two different sets of residues as follows:
+
+\begin{verbatim}
+
+SURROUND_CHARS DE ALL
+SURROUND_CHARS DEHKR ALL
+
+\end{verbatim}
+
+This would result in D and E characters being partitioned from the rest as well
+as D E H K R characters (see Example output).
+
+\subsection{More complex effects - Text Lines, and Masks}
+
+Text, lines and masking are meant to be used to annotate the multiple
+alignment. The TEXT command allows any piece of text to be located
+anywhere on the alignment. Clearly, however it makes little sense to
+superimpose the text over the alignment though this can be done! Accordingly,
+you must first make a space to put the text in. Usually, this will be a
+few lines below the multiple sequence alignment, but you may want to
+add text at the top, or somewhere in between two sequences. You can make
+space in two ways. Either by editing the block-file to introduce "dummy"
+sequences at the locations you want, or by making use of the ADD\_SEQ\
+command.
+
+The ADD\_SEQ\ command has two arguments, the sequence after which you want
+further sequences to be added, and how many blank sequences you need.
+Thus, we can reserve space for 5 lines of text underneath a 10 sequence
+multiple alignment with the following command.
+
+ADD\_SEQ\ 10 5
+
+we can then put text below the alignment at the 20th residue.
+
+TEXT 20 13 "Active Site His"
+
+or any other position.
+
+Similarly, we could draw a vertical line to point out which residue we mean
+
+LINE LEFT 20 13 11
+
+And change the font of the text to number 7 (whatever that has been set to):
+
+FONT\_RESIDUE\ 20 13 7
+
+You can have multiple ADD\_SEQ\ commands, but they must occur in sequence order.
+Thus:
+
+\begin{verbatim}
+ADD_SEQ 0 5
+ADD_SEQ 5 12
+\end{verbatim}
+is legal.
+But
+\begin{verbatim}
+ADD_SEQ 5 12
+ADD_SEQ 0 5
+\end{verbatim}
+
+Is NOT!! NO CHECKING IS performed by the program for this error - so beware!
+
+Note that add\_seq\ commands refer to the actual sequence number as
+implied by the block file, not the number after applying the add\_seq\
+command. Thus, for a four sequence block file, if you want to add
+space for three sequences before sequence 1 and two sequences after
+sequence 3, the commands would be:
+
+\begin{verbatim}
+ADD_SEQ 0 3
+ADD_SEQ 3 2
+\end{verbatim}
+
+Text added with the TEXT command will not be split across page breaks,
+so you may in some circumstances need to fiddle a little with the
+location/pointsize for the text to get the desired result.
+
+Masking is a technique for drawing irregular shaped outlines, or
+shaded regions - this should not be confused with the MASK family of commands
+described below. For example a histogram can be added to the bottom of
+an alignment by first defining some dummy sequences in the block-file
+that have letters building up the shape of the histogram, then using
+the SURROUND\_CHARS\ or SHADE\_CHARS\ commands together with the SUB\_CHARS\
+command to produce the desired effect. An example of this operation
+being used to show frequencies of secondary structure predictions is
+shown in example1.als and in the Protein Engineering paper.
+
+\section{Using Colour}
+
+Version 1.4 includes commands to allow the independent colouring of
+characters, or their backgrounds. Colours are defined in a similar
+manner to fonts using the DEFINE\_COLOUR\ command (American spelling also
+allowed). For example:
+
+DEFINE\_COLOUR\ 7 1 0 0
+
+defines colour number 7 to be red - see
+\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1}
+for full details of
+this command. Colours 99 and 100 are pre-defined to white and black.
+ALSCRIPT assumes the paper colour is white.
+
+The command to colour the text of a character or text string is:
+
+CCOL\_CHARS\
+
+the command to colour the background of a character is
+
+SCOL\_CHARS\
+
+both have similar syntax to the FONT\_CHARS command.
+
+COLOUR\_REGION\ and COLOUR\_RES\ have similar syntax to SHADE\_REGION\ and
+SHADE\_RES.
+
+An example command file that uses colour is shown in example3.als.
+
+\section{The MASK command family}
+
+The idea behind the MASK command is to build up a set of character
+positions that will subsequently be boxed, shaded, set in a particular
+font, etc. For example, lets say we want to box the most frequently
+occuring character at each position in an alignment.
+
+The command
+
+mask SETUP
+
+tells ALSCRIPT to prepare a mask.
+
+mask FRE ALL
+
+specifies that the most frequently occuring character at each position
+in the alignment will be masked. This command can be restricted to a
+region of the alignment using: mask FRE sx sy ex ey, where sx etc
+define the region in the same way as for font\_region\ and other commands.
+
+mask BOX ALL
+
+Tells ALSCRIPT to create the boxing lines that will separate the masked
+characters from non-masked characters - this command may also be
+restricted to a region of the alignment.
+
+The mask can be reset for re-use using the command:
+
+mask RESET
+
+Two further commands define which characters can be used when
+calculating the mask. This allows gap-characters, or other amino acids
+to be excluded from the calculation to avoid unwanted boxing.
+
+mask LEGAL "AVLI"
+
+defines the AVL and I as the only characters that will be used when
+calculating the mask.
+
+mask ILLEGAL ".-\_"
+
+defines .- and \_\ as characters that will not be used when calculating
+the mask. NOTE: the blank character " " cannot be defined in this way.
+To avoid boxing " " characters substitute blanks for something else
+(using SUB\_CHARS),\ calculate the mask, then substitute back.
+
+\subsection{Summary of mask commands}
+
+mask SETUP
+\# Prepares for masking
+
+mask LEGAL $<$qstring$>$
+\# defines characters to include in ID or FRE calcs - optional.
+
+mask ILLEGAL $<$qstring$>$
+\# defines characters to exclude in ID or FRE calcs - optional.
+
+mask ID ALL N
+\# Calculates a mask that flags the character that occurs at least N
+times at a position. The word ALL can be substituted by four numbers
+that define a region of the alignment.
+
+mask FRE ALL
+\# calculates a mask that flags the most frequently occuring amino acid
+at each position. ALL may be replaced by four numbers defining a region
+of the alignment.
+
+Multiple mask FRE or mask ID commands may be applied, using different
+LEGAL and ILLEGAL character definitions. In this way more complex
+effects can be built up.
+
+The mask command also allows characters that are identical to one
+sequence to be masked.
+
+mask AGREE ALL N
+
+will mask all positions that are identical to the Nth sequence. Thus,
+for sequences that are very similar to a newly sequenced sequence, all
+characters identical to the new sequence can be boxed or shaded, or set
+in a different font or colour etc...
+
+mask NOT ALL
+
+allows the mask to be inverted. Thus, all positions that are NOT in the
+mask now form the mask. So, having done a mask AGREE, a mask NOT will
+allow the positions that are not identical to the selected sequence to
+be highlighted or substituted.
+
+mask SUB ALL $<$char$>$
+
+substitutes all characters in the mask with the character $<$char$>$.
+
+mask REGION ALL
+applies a mask to all residues in the defined region.
+
+The following effects can now be applied to the masked characters:
+
+mask BOX ALL
+\# boxes the masked residues - ie surrounds them by lines.
+
+mask SHADE ALL $<$grey$>$
+\# shades the masked residues by grey value.
+
+mask FONT ALL $<$fontnum$>$
+\# Uses font fontnum to output the masked residues.
+
+mask INVERSE ALL
+\# Inverts the masked characters - ie outputs them in white.
+
+mask CCOL ALL $<$colnum$>$
+\# outputs the masked characters in the defined colour.
+
+mask SCOL ALL $<$colnum$>$
+\# outputs the backgrounds of the masked characters in the defined
+colour.
+
+In all commands, the word ALL can be replaced by four numbers defining
+the region to which the command is applied.
+
+mask RESET
+\# resets the entire mask for re-use
+
+
+\section{Printing ALSCRIPT Files}
+
+ALSCRIPT produces files in PostScript which may be printed on a PostScript
+printer (e.g. an Apple LaserWriter). If you don't have a PostScript printer,
+then you may still be able to use ALSCRIPT if you get hold of the
+GhostScript software. This is a free package that interprets PostScript
+commands and can produce output on a large number of different types of
+printer. GhostScript runs on most hardware types (including PCs) and can also
+display output to the screen. The package can be obtained from many different
+sites on the Internet (In the UK try src.doc.ic.ac.uk).
+
+The actual command you need to type to send a PostScript file to the printer
+will depend on your system. Consult your system manager for help.
+
+Be warned, ALSCRIPT can create extremely large PostScript files if
+lots of boxing and shading is done on big alignments. On older
+printers such output may take a long time to process.
+
+\section{Conclusion}
+
+ALSCRIPT provides a powerful set of formatting and editing commands
+specifically tailored for multiple sequence alignments. It is best
+used in conjunction with a PostScript previewer such as Sun's
+PageView or GhostView since this allows the effect of changing a command to be
+seen quickly. In the absence of such a tool, simpler effects can be
+tested out without destroying too many trees in the Laser Printer!
+
+Like most programs, ALSCRIPT is evolving as I find new problems to display, so
+if you have any suggestions - I shall endeavour to include them in a later
+version.
+
+\section{Appendices}
+
+\subsection{ALSCRIPT Command Summary}
+\label{app1}
+
+WARNING: Very little error checking is performed on command input. If
+you give the wrong number of arguments to a command, then unexpected
+things may happen, or the program will crash very inelegantly. I hope
+to fix this in the next version of the program, in the meantime, make sure
+you give the correct number of arguments to each command.
+
+All commands up to the first space character may be entered in UPPER
+or lower case or MiXEd case. Qualifiers for commands (e.g. REL) must
+be written in UPPER case.
+
+Command Reference:
+
+\begin{verbatim}
+<int> = enter an integer (e.g. 240)
+<float> = enter a floating point number (e.g. 0.45)
+<string> = enter a string (e.g. ARNDql)
+<qstring> = enter a quoted string (e.g. "Active Site")
+<char> = enter a single character.
+\end{verbatim}
+
+\subsubsection{STEP 1 COMMANDS}
+
+These all refer to either system settings - e.g. the maximum allowed
+sequence length, or to general page layout features. e.g. the longest
+and shortest side of the paper on which you are plotting.
+
+\subsubsection{REQUIRED STEP 1 COMMANDS}
+
+BLOCK\_FILE\ $<$string$>$
+
+Gives the name of the file that contains the multiple sequence alignment
+to be formatted. File names should be fully qualified i.e. not
+relative to the current directory. If no block file command is given,
+ALSCRIPT will expect to read the block file from standard input.
+
+OUTPUT\_FILE\ $<$string$>$
+
+Defines the output file name. This command should be near the beginning of
+the command list.
+e.g. OUTPUT\_FILE\ Figure1.ps
+
+You MUST define an output file unless the -p option
+(See \hyperref{Alternative ways of invoking ALSCRIPT}{Section }{ }{app7})
+is used.
+
+DEFINE\_FONT\ $<$int$>$ $<$string$>$ ($<$int$>$/DEFAULT)/(REL $<$float$>$)
+
+Defines a font to use later:
+e.g.
+\begin{verbatim}
+DEFINE_FONT 0 Helvetica 10
+DEFINE_FONT 2 Times-Roman 2
+\end{verbatim}
+
+defines font number 0 to be 10 point Helvetica, and font number 2 to be
+2 point Times-Roman. Font 0 is always used as the default font. You MUST
+define at least the font 0 font.
+
+DEFINE\_FONT\ 3 Times-Roman DEFAULT
+
+sets font 3 to be Times-Roman at whatever the default pointsize
+is as set by the POINTSIZE command.
+
+DEFINE\_FONT\ 4 Helvetica REL 0.5
+
+sets font 4 to be helvetica at half the default pointsize.
+
+NOTE: Font names must be written exactly as shown in
+\hyperref{PostScript Fonts}{Section }{ }{app3}.
+
+
+SETUP
+
+Signals the end of the STEP 1 commands.
+
+\subsubsection{OPTIONAL STEP 1 COMMANDS}
+
+
+ADD\_SEQ\ $<$int$>$ $<$int$>$
+
+Allows extra sequence positions to be created in an existing alignment. This
+permits additional annotations to be interspaced either above, below, or
+anywhere in the middle of an alignment. For example:
+
+ADD\_SEQ\ 0 10
+
+would create an additional 10 sequences - all set to the blank character
+before the first sequence in the block file that has been read in.
+
+ADD\_SEQ\ 3 1
+
+would add an extra sequence after sequence 3.
+
+IMPORTANT: If you use the ADD\_SEQ\ facility to add sequences anywhere except
+after the last sequence, then remember that the sequence
+numbers will alter. All formatting commands that follow this command
+must use the new sequence numbering. Thus in the first example:
+
+ADD\_SEQ\ 0 10
+
+what was sequence number 1 becomes sequence 11. Sequences 1-10 are
+the new blank sequences to be used for annotation. Note that the
+sequence numbers only change for commands AFTER the SETUP command,
+thus, multiple add\_seq\ commands refer to the sequence number as
+implied by the block file.
+
+POINTSIZE $<$int$>$
+
+Defines the pointsize to be used to scale the plot and space the characters.
+Default is 10 point.
+
+NUMBER\_SEQS
+
+If present, then the sequence number is output with the identifier
+code. This is useful for finding the coordinates of residues to box
+or otherwise highlight.
+
+LANDSCAPE
+
+Specifies that alignments will be plotted with the longest paper axis
+horizontal. (Can get longer alignments on a page this way).
+
+PORTRAIT
+
+Specifies that alignments will be plotted with the longest paper axis vertical
+(can get more sequences on a page this way).
+
+IDENT\_WIDTH\ $<$int$>$
+
+Units are characters.
+
+Reserves $<$int$>$ characters at left of every page for plotting
+identifiers. Note that not all this space need be used, if a smaller
+pointsize is used to plot out the identifier codes, than is used for
+the main alignment.
+
+LINE\_WIDTH\_FACTOR\ $<$float$>$
+
+Value greater than 0 that scales the default line width. The linewidth
+is obtained by multiplying the pointsize by this factor.
+
+
+X\_SPACE\_FACTOR\ $<$float$>$
+
+Y\_SPACE\_FACTOR\ $<$float$>$
+
+This determines the spacing between adjacent residues in the X and Y
+directions. The spacing is calculated as: POINTSIZE + POINTSIZE *
+X\_SPACE\_FACTOR or POINTSIZE + POINTSIZE * Y\_SPACE\_FACTOR\ as
+appropriate. Defaults are 0.2 and 0.0 respectively.
+
+X\_SHIFT\_FACTOR\ $<$float$>$
+
+Y\_SHIFT\_FACTOR\ $<$float$>$
+
+These determine the shift relative to the residue drawing position
+that is given to the boxing lines. The shift is calculated as
+follows
+
+(POINTSIZE + POINTSIZE * X\_SPACE\_FACTOR)\ * X\_SHIFT\_FACTOR\
+similarly for Y\_SHIFT\_FACTOR.
+
+The defaults are 0.3 and 0.0 respectively.
+
+Fiddling with the X\_SPACE/SHIFT\ values is useful to fine tune the
+appearance of the alignment.
+
+
+MAX\_INPUT\_LEN\ $<$int$>$
+
+Units are characters.
+Defines the maximum number of characters possible in the input line
+length. This must be greater than the maximum number of sequences
+(MAX\_NSEQ).
+
+e.g. MAX\_INPUT\_LEN\ 600
+
+Increases the default value of 500 characters to 600 characters.
+
+MAX\_NSEQ\ $<$int$>$
+
+Units are characters. Defines the maximum number of sequences that
+may be read by the program. This parameter has a large default (500).
+You may need to reduce it on computers with small memories.
+
+MAX\_ILEN\ $<$int$>$
+
+Units are characters.
+The maximum length allowed for a sequence identifier code.
+
+MAX\_SEQ\_LEN\ $<$int$>$
+
+Defines the maximum length allowed for a sequence alignment - this may
+need to be reduced from the 8000 default value on smaller computers.
+
+X\_OFFSET\ $<$int$>$
+Units of points (1/72 inch).
+
+Defines the offset along the X-axis that the alignments will be shifted prior
+to printing. Fiddle with this value to get a nice offset from the bottom left
+hand corner of the page if your page size is not A4.
+
+Y\_OFFSET\ $<$int$>$
+Units of points (1/72 inch).
+
+As for X\_OFFSET,\ only Y axis.
+
+MAX\_SIDE\ $<$int$>$
+Units of inches.
+
+Defines the length of the longest side of the printer page.
+
+MIN\_SIDE\ $<$int$>$
+Units of inches.
+
+Defines the length of the shortest side of the printer page.
+
+VERTICAL\_SPACING\ $<$int$>$
+
+Defines the vertical spacing in character units between blocks of sequences
+when more than one block will fit on a page - default is 0.
+
+
+DEFINE\_COLOUR\ $<$int$>$ $<$float$>$ $<$float$>$ $<$float$>$
+
+DEFINE\_COLOR
+
+Defines a colour - the first number is a number by which the colour will
+be referred. The following three numbers are the intensities of red,
+green and blue respectively. Thus:
+
+DEFINE\_COLOUR\ 1 0 0.2 0.8
+
+sets colour number 1 to be a colour with no red, 0.2 green and 0.8 blue.
+The exact appearance of this colour will depend on the output device.
+If you find suitable combinations of colours for your printer, then
+please let me know and I shall distribute your suggestions with the
+program.
+
+DO\_TICKS
+
+If present, then tick marks are drawn below the numbers at the top of the page.
+Otherwise no ticks are shown.
+
+NUMBER\_INT\ $<$int$>$
+
+Specifies the interval for writing residue position numbers. Default is 10
+
+NO\_NUMBERS
+
+Switches all residue numbering off.
+
+
+\subsubsection{STEP 2 COMMANDS}
+
+
+All these are optional formatting commands.
+
+IMPORTANT PLEASE READ THIS NOTE:
+
+For those commands that accept region definitions (e.g. SURROUND\_CHARS) it
+is easiest to think of the region being defined in terms of X and Y
+coordinates, where X is the sequence residue coordinate and Y is the
+sequence number coordinate. Thus 3 7 means the 3rd residue in sequence 7.
+3 7 12 42 means the rectangular box bounded by residue 3 of sequence 7 and
+residue 12 of sequence 42.
+
+SURROUND\_CHARS\ $<$string$>$ ALL
+
+Draw lines round, but not between the characters that are in the string.
+e.g.
+
+SURROUND\_CHARS\ GP ALL
+
+will draw lines round all G and P characters in the alignment, but not
+between adjacent G and P characters.
+
+SURROUND\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+Similar command, but the surrounding is restricted to the region defined by
+the four integers.
+
+e.g.
+
+SURROUND\_CHARS\ ILVW 3 12 7 32
+
+would surround ILVW characters that occur in the region defined
+from residue positions 3-7 of sequences 12 to 32.
+
+SHADE\_CHARS\ $<$string$>$ ALL $<$float$>$
+
+Shade all characters in the $<$string$>$ by the grey value given by $<$float$>$.
+e.g.
+
+SHADE\_CHARS\ GP ALL 0.5
+
+would shade all G and P characters in the alignment by the grey value 0.5.
+
+SHADE\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$float$>$
+
+restricts the shading to the region defined by the four integers. Thus
+
+SHADE\_CHARS\ ILVW 3 12 7 32 0.7
+
+would shade I L V and W characters from residues 3-7 of sequences 12-32
+inclusive with a grey value of 0.7.
+
+FONT\_CHARS\ $<$string$>$ ALL $<$int$>$
+
+e.g.
+
+FONT\_CHARS\ GP ALL 7
+
+would use font 7 to write out all G and P characters. Font 7 MUST have been
+defined using the DEFINE\_FONT commands above.
+
+FONT\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+Similar to previous command, but restricts the effect to the region defined
+by the first four integers. The font must have been defined by the
+DEFINE\_FONT\ command.
+
+e.g.
+
+FONT\_CHARS\ ILVW 3 45 9 70 7
+
+Would set the font to 7 for I L V and W characters for residues 3-9 of
+sequences 45-70 inclusive. The font must have been defined by the
+DEFINE\_FONT\ command.
+
+FONT\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+Define the font to use throughout the region specified by the first four
+integers.
+
+e.g.
+
+FONT\_REGION\ 3 12 20 40 10
+
+Use font 10 for residues from residues 3-20 of sequences 12-40. The font
+must have been defined using the DEFINE\_FONT command.
+
+FONT\_RESIDUE\ $<$int$>$ $<$int$>$ $<$int$>$
+
+Set the font for use with a single residue position - most useful when used
+with the TEXT command.
+
+e.g.
+
+FONT\_RESIDUE\ 3 7 2
+
+Use font 2 for residue 3 of sequence 7. Font 2 must have been defined using
+the DEFINE\_FONT\ command.
+
+LINE $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+There are four commands of this type for drawing horizontal or vertical lines
+on the alignment.
+
+LINE LEFT $<$int$>$ $<$int$>$ $<$int$>$
+
+Draw a line to the left of the character positions indicated.
+
+e.g.
+
+LINE LEFT 3 12 24
+
+Draw a vertical line starting at residue 3 of sequence 12 and ending at
+residue 3 of sequence 24.
+
+LINE TOP 3 12 24
+
+Draw a horizontal line above the character positions from residue 3 of
+sequence 12 to residue 24 of sequence 12.
+
+Similar commands are:
+
+LINE BOTTOM $<$int$>$ $<$int$>$ $<$int$>$ Draw a line at bottom of character position.
+
+LINE RIGHT $<$int$>$ $<$int$>$ $<$int$>$ Draw a line at right of character position.
+
+BOX\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+Draw a box around the region indicated by the four integers.
+
+e.g.
+
+BOX\_REGION\ 2 5 30 7
+
+Would box from residue 2 of sequence 5 to residue 30 of sequence 7.
+
+SHADE\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$float$>$
+
+Shade the region indicated by the integers with the grey value shown by the
+float.
+e.g.
+
+SHADE\_REGION\ 30 40 35 46 0.2
+
+Would shade from residue 30-35 of sequences 40-46 with a grey value of 0.2.
+
+SHADE\_RES\ $<$int$>$ $<$int$>$ $<$float$>$
+
+Shade just one amino acid with the grey value.
+
+e.g.
+
+SHADE\_RES\ 3 4 0.7
+
+Shades residue 3 of sequence 7. (Note: this can also be achieved with the
+
+SHADE\_REGION\ command, but requires 2 extra numbers)
+
+TEXT $<$int$>$ $<$int$>$ $<$qstring$>$
+
+Place the text string at the location indicated.
+
+e.g.
+
+TEXT 30 70 "Active Site His"
+
+would put the text Active Site His starting at position 30 of sequence
+70. (Use FONT\_RESIDUE\ or FONT\_REGION\ commands to set the font of the
+text). Text added with the TEXT command will not be split across page
+breaks, so you may in some circumstances need to fiddle a little with
+the location/pointsize for the text to get the desired result.
+
+ID\_FONT\ ALL $<$int$>$
+
+Set the font for all identifier codes to the font number shown by $<$int$>$.
+e.g.
+
+ID\_FONT\ ALL 3
+
+Would set all the identifier codes to font 3.
+
+ID\_FONT\ $<$int$>$ $<$int$>$
+
+Set the font for a specific identifier to font number.
+e.g.
+
+ID\_FONT\ 12 4
+
+Use font 4 for the identifer of sequence 12, default font for all other
+identifiers.
+
+SUB\_CHARS\ ALL $<$char$>$ $<$char$>$
+
+Substitute the characters indicated.
+
+e.g.
+
+SUB\_CHARS\ ALL + *
+
+would change all occurences of + to * in the alignment.
+
+SUB\_CHARS\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$char$>$ $<$char$>$
+
+restrict the substitution to the region shown.
+
+e.g.
+
+SUB\_CHARS\ 1 1 7 8 \% *
+
+would substitute * for \% from residue 1-7 of sequences 1-8.
+NOTE: To substitute for or with the space character use the word SPACE.
+e.g. to change all space characters to -.
+
+SUB\_CHARS\ ALL SPACE -
+
+SUB\_ID\ $<$int$>$ $<$qstring$>$
+
+Replace the numbered identifier by the string.
+e.g.
+
+SUB\_ID\ 34 "Predicted Secondary Structure"
+
+would replace whatever the identifier of sequence 34 was, by the text shown.
+This is useful when used in conjunction with the ADD\_SEQ\ command shown under
+the STEP 1 commands.
+
+INVERSE\_CHARS\ $<$string$>$ ALL/Range (similar syntax to FONT\_CHARS\ but no
+font number)
+
+Print the selected characters in white. This clearly will only work
+if you first use the SHADE\_CHARS command to shade the characters with
+something other than white.
+
+CCOL\_CHARS\ $<$string$>$ ALL $<$int$>$
+
+Colour all characters in the $<$string$>$ by the colour defined by $<$int$>$.
+
+e.g.
+
+CCOL\_CHARS\ GP ALL 12
+
+would colour all G and P characters in the alignment by the colour 12.
+This colour MUST have been defined by the DEFINE\_COLOUR\ command.
+
+CCOL\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+restricts the colouring to the region defined by the four integers. Thus
+
+CCOL\_CHARS\ ILVW 3 12 7 32 7
+
+would colour I L V and W characters from residues 3-7 of sequences 12-32
+inclusive with the colour 7.
+
+SCOL\_CHARS:\ This has identical syntax to SCOL\_CHARS,\ but colours the
+background of the character, rather than the letter itself.
+
+COLOUR\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$
+
+COLOR\_REGION
+
+Colour the region indicated by the integers with the colour number given
+as the last number.
+
+e.g.
+
+COLOUR\_REGION\ 30 40 35 46 2
+
+Would colour from residue 30-35 of sequences 40-46 with the colour 2.
+
+COLOUR\_RES\ $<$int$>$ $<$int$>$ $<$int$>$
+
+Colour just one amino acid with the defined colour.
+
+e.g.
+
+COLOUR\_RES\ 3 4 7
+
+Colours residue 3 of sequence 7. (Note: this can also be achieved with the
+COLOUR\_REGION\ command, but requires 2 extra numbers)
+
+
+\subsection{AMPS Block file format}
+\label{app2}
+
+The first part of a block-file contains the identifier codes of the
+sequences that are to follow. Each code is prefixed by the $>$ symbol, codes
+must not contain spaces.
+
+e.g.
+\begin{verbatim}
+>HAHU
+>Trypsin
+>A0046
+>Seq1
+
+\end{verbatim}
+
+etc.
+
+ALSCRIPT counts the number of $>$ symbols in the beginning of the file
+until a * symbol is found. The * signals the beginning of the
+multiple alignment which is stored VERTICALLY, thus columns are
+individual sequences, whilst rows are aligned positions. The * symbol
+must lie over the first sequence. A further star in the same column
+signals the end of the alignment. ALSCRIPT uses the number of $>$
+symbols at the beginning of the file to work out how many columns to
+read from the * position. It is therefore important that the only $>$
+symbols in the file are those that define the identifiers, and the
+only * symbols are those defining the start and end of the multiple
+alignment. The block file can contain additional text, providing that
+there are no more $>$ or * symbols in the file than those used to define
+the identifiers or alignment start and end.
+
+A simple, small block-file is shown here.
+
+\begin{verbatim}
+>Seq_1
+>A0231
+>HAHU
+>Four_Alpha
+>Globin
+>GLobin_C
+*
+ARNDLQ
+AAAAAA
+PPPPPP
+PP PPP
+WW WWW
+LLLLLL
+IIVVLL
+*
+\end{verbatim}
+
+
+\subsection{PostScript Fonts}
+\label{app3}
+\begin{verbatim}
+Times-Roman,
+Times-Italic,
+Times-Bold,
+Times-BoldItalic,
+Helvetica,
+Helvetica-Oblique,
+Helvetica-Bold,
+Helvetica-BoldOblique,
+Courier,
+Courier-Oblique,
+Courier-Bold
+Courier-BoldOblique,
+AvantGarde-Book,
+AvantGarde-BookOblique,
+AvantGarde-Demi,
+AvantGarde-DemiOblique,
+Bookman-Demi,
+Bookman-DemiItalic,
+Bookman-Light,
+Bookman-LightItalic,
+Helvetica-Narrow,
+Helvetica-Narrow-Bold,
+Helvetica-Narrow-BoldOblique,
+Helvetica-Narrow-Oblique,
+NewCenturySchblk-Roman,
+NewCenturySchlbk-Bold,
+NewCenturySchblk-Italic,
+NewCenturySchblk-BoldItalic,
+Palatino-Roman,
+Palatino-Bold,
+Palatino-Italic,
+Palatino-BoldItalic
+ZapfChancery-MediumItalic.
+Symbol
+\end{verbatim}
+
+\subsection{386 DOS installation}
+\label{app4}
+
+
+IMPORTANT - The programs on this disk will ONLY WORK on a PC with a 386
+or better processor. See the Technical Notes section for details of why.
+
+Directions:
+
+\begin{enumerate}
+
+\item
+ Create a directory on your hard disk.
+ e.g. mkdir ALSCRIPT.
+
+\item
+ Copy the Contents of the floppy disk into this directory.
+\begin{verbatim}
+ e.g. copy a:*.* c:\alscript.
+\end{verbatim}
+
+\item
+ Edit your AUTOEXEC.BAT file and add
+\begin{verbatim}
+ C:\ALSCRIPT to your path.
+\end{verbatim}
+
+\item
+ Edit your AUTOEXEC.BAT file and add the following two lines.
+ set DOS4GVM=@ALSCRIPT.VMC
+ set DOS4G=quiet
+\end{enumerate}
+
+The first line is an instruction to read instructions from the file
+ALSCRIPT.VMC. This sets up a permanent swap file on your hard disk.
+By default, the swap file is about 12MBytes in size. If you do not have
+this much free space on your disk, then edit the ALSCRIPT.VMC file
+to reduce the swap file size, or alternatively, do not put this line
+in your autoexec.bat.
+
+The programs will run without this swap file, but you will be limited in
+ the size of alignment you can process by the amount of RAM you have
+installed. I have only tested this program on a 486/33 with 8MBytes RAM
+and a 386/33 with 4MBytes so I do not know the practical limitations of
+machines with smaller memories. Any feedback would be appreciated.
+
+5. Type AUTOEXEC.BAT to initialise the changes, or better still, reset
+the computer.
+
+6. You should now be able to run all three programs in the package from
+anywhere on your disk. msf2blc, clus2blc and alscript. If you get
+memory allocation errors when you try to run alscript, then use the
+MAX\_NSEQ\ and MAX\_SEQ\_LEN\ commands to reduce the default limits. If the
+program still won't run, then think about buying some more memory!!
+
+The programs msf2blc and clus2blc should run OK, but if you try to
+process alignments that are too large for your computer, you may get a
+"malloc error" which will stop the program. If this happens and you are
+not using the virtual memory option discussed above, then try adding the
+line set DOS4GVM=@filename to your autoexec.bat file. If you
+don't have enough disk space to do this, then buy a bigger disk, or more
+memory.
+
+\subsection{TECHNICAL NOTES}
+
+The executables included in this package were compiled with the WATCOM C
+compiler. This is a full 32 bit compiler that makes good use of the 386
+processor and does not work on the 16 bit 286. It also has the
+advantage of allowing the flat memory model to be used. In practice
+this means that porting programs like alscript from Unix computers like
+the Sun, is straightforward. In order to access the memory of the
+computer in this way, an extra program called a dos extender is required
+- this is called DOS4GW.EXE. DOS4GW is automatically invoked every time
+you run one of the programs and is responsible for managing the memory and
+creating the swap file discussed above.
+
+\subsection{Unix Installation}
+\label{app5}
+
+ALSCRIPT is distributed with executables for Sun (SunOS 4.1.3), Silicon
+Graphics (IRIX 5.3), DEC ALPHA OSF/1 and Sun Solaris (2.4). The executables
+are stored in the subdirectories bin/sun, bin/sgi, bin/osf and bin/sol. If
+these are OK for your system, then just add the apporpriate directory to your
+path, or put links to /usr/local/bin or somewhere that is on all users paths.
+
+The source code for ALSCRIPT is contained in a directory hierarchy.
+The top directory contains a README file and the BUILD script.
+Subdirectories are: {\bf examples} which contains example command and
+alignment files, {\bf doc} which contains \LaTeX and PostScript copies
+of the manual - a subdirectory of this contains an HTML version of the
+manual, and {\bf src} which contains the source code and Makefiles for
+the package. There may also be a directory called {\bf bin}. If
+present this will contain subdirectories with executables for the
+programs in the package. Makefiles to build alscript, msf2blc,
+clus2blc and alsnum are included in the {\bf src} directory. Versions
+for Sun (acc compiler .sun), Silicon Graphics (.sgi), DEC OSF/1 (.osf)
+are included.
+
+There is a utility csh script called BUILD. Simply type ./BUILD sun to
+compile alscript on the Sun, ./BUILD sgi for Silicon Graphics or BUILD
+gcc for use with gcc compiler. See instructions in the file BUILD.
+The BUILD script will create a /bin directory and subdirecotry
+if not already present. You can create makefiles for different computers
+and the BUILD script should still function.
+
+
+\subsection{VAX/VMS Installation}
+\label{app6}
+
+The standard VAX C compiler is not ANSI. Accordingly, ALSCRIPT will require
+changes to the source code to compile on a VAX.
+
+The DEC C++ compiler works OK for alscript. Alscript will also compile
+on Dec ALPHA under OpenVMS. A descrip.mms file is included for this
+purpose.
+
+{\em WARNING: I've not tested Version 2.0 of ALSCRIPT on VMS}
+
+\subsection{Alternative ways of invoking ALSCRIPT}
+\label{app7}
+
+The documentation above describes the interactive mode of running ALSCRIPT.
+However, it may be more convenient to run the program as a pipe under
+Unix or MS-DOS. Examples are shown here.
+
+ALSCRIPT is a program for producing pretty versions of multiple
+sequence aligments. ALSCRIPT will also format single sequences. A
+full description of the program is given in the file "alscript.doc".
+
+Ways of running alscript:
+
+\begin{enumerate}
+
+\item
+ Interactive mode: just type alscript.
+ You will be prompted for a command file name. The command file will
+ define the AMPS blocfile, and name of the file to store the PostScript
+ output - see alscript.doc for details.
+
+\item
+ alscript $<$command\_file$>$\ has same effect as 1, But does not prompt for
+ the command file
+ e.g. alscript example1.als
+
+\item
+ alscript -q $<$ $<$blocfile$>$ $>$ $<$PostScript$>$
+ Quick mode - uses default commands, reads alignment from stdin,
+ writes PostScript to stdout. This mode creates a command file
+ called ALPSQ.COM.
+
+ e.g. alscript -q $<$ example1.blc $>$ example1.ps
+
+\item
+ alscript -f $<$command\_file$>$
+ Similar effect to 2.
+
+\item
+ alscript -f $<$command\_file$>$\ -s
+ Silent operation: No messages are written to stderr, unless fatal.
+ Silent operation may be toggled by the silent\_mode\ command
+ in the command file.
+
+\item
+ alscript -f $<$command\_file$>$\ -p $<$ $<$blocfile$>$ $>$ $<$PostScript$>$
+ Make alscript work like a pipe - blocfile is read from stdin,
+ postscript is written
+ to stdout. Messages are written to stderr. To supress messages include
+ the -s flag too
+
+ e.g. alscript -f example1.als -p -s $<$ example1.blc $>$ example1.ps
+
+\end{enumerate}
+
+Using alscript as a pipe has the advantage of allowing the blocfile to
+be created on the fly by the programs msf2blc or clus2blc. For example
+if we have a GCG .msf file called "pileup.msf" we can run alscript with
+default shading/fonts and send the results straight to the PostScript
+printer "lpr" as follows:
+
+msf2blc -q $<$pileup.msf | alscript -q -s | lpr
+
+\subsection{Program Crashes and Known Bugs}
+\label{app8}
+
+We've used ALSCRIPT on Sun Workstations and Silicon Graphics for some
+time, with very large alignments and command files with thousands of
+commands. All seems to work OK, the program has not crashed on us at
+all!!
+
+However, the command interpreter in ALSCRIPT is very simple and
+the program will crash if you give any command the wrong number of
+arguments (e.g. leaving out the shade value in a shade\_chars\ command).
+
+If you do make the program crash, have checked all the documentation
+and your numbers, and the program still crashes. Then send me
+the command file and block file that causes the crash and I will try
+to investigate.
+
+Suggestions for improvements to the program are always welcome.
+
+\subsection{Wish List for next version!!}
+\label{app9}
+
+A command interpreter that does more error checking will be included.
+Currently, no checking is done to make sure that the correct number of
+arguments are given to a command.
+
+Sequences will be able to be given unique labels and region commands refer
+to these labels or ranges of labels. This will permit a sequence to be
+deleted or added to the alignment without having to update the .als file.
+
+The relative numbering option will be extended to allow numbering relative
+to a position. e.g. 456+7 would be 7 residues after position 456. This
+will allow annotation of positions that may be in insertions relative to the
+reference sequence.
+
+Special TEXT commands will be extended to allow alternative shapes to
+be drawn and scaled in various ways.
+
+Tree drawing and generalised graphics. An option to draw arbitrary lines
+on an alignment will be added. This will permit line graphics to be added
+to an alignment. The initial reason for this will be to show dendrograms
+(trees) alongside the alignment, but simple line graphs could also be plotted
+under the alignment.
+
+Fiddle factors will be introduced to allow fine positioning of
+individual characters. For example, if you like your ``I'' characters
+to be centred rather than left justified, this will be possible.
+
+In single\_page mode, it will be possible to add arbitrary text to an
+alignment for final annotation, e.g. titles etc.
+
+Variable height/width sequence lines will be permitted (maybe).
+
+\subsection{Acknowledgements}
+
+I thank all those who have emailed me with suggestions for
+improvements to alscript. I've tried to include some of these in the
+current distribution (e.g. screening).
+
+\subsection{References}
+\label{app10}
+
+\begin{verbatim}
+
+1. Barton, G. J. (1993),
+ "ALSCRIPT A tool to format multiple sequence alignments",
+ Protein Engineering, Volume 6, No. 1, pp.37-40.
+
+2. Barton, G. J. (1990),
+ "Protein Multiple Sequence Alignment and Flexible Pattern Matching",
+ Methods in Enzymology,
+ 183,403-428.
+
+3. Barton, G. J. and Sternberg, M. J. E. (1987),
+ "A Strategy for the Rapid Multiple Alignment of Protein Sequences:
+ Confidence Levels From Tertiary Structure Comparisons",
+ Journal of Molecular Biology,
+ 198,327-337
+
+4. Higgins, D. G. and Sharp, P. M. (1989),
+ "Fast and sensitive multiple sequence alignments on a microcomputer",
+ CABIOS,
+ 5,151--153
+
+5. Devereux, J. Haeberli, P. Smithies, O. (1984),
+ "A comprehensive set of sequence analysis programs for the VAX",
+ Nucleic Acids Res.
+ 12, 387-395
+
+6. Livingstone, C. D. and Barton, G. J. (1993),
+ "Protein Sequence Alignments: A Strategy for the Hierarchical analysis
+ of residue conservation"
+ Computer Applications in the Biosciences,
+ 9, 745-756.
+
+\end{verbatim}
+\end{document}
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+#define noname_width 37
+#define noname_height 15
+static char noname_bits[] = {
+ 0x86,0x99,0x0c,0x70,0x0c,0x86,0x89,0x08,0x60,0x04,0x86,0x83,0x00,0xc0,0x02,
+ 0x46,0x83,0x00,0xc0,0x03,0x46,0x82,0x00,0x80,0x01,0xe6,0x87,0x38,0x82,0x03,
+ 0x26,0x86,0x18,0x40,0x07,0x36,0x8d,0x18,0x60,0x06,0x86,0x81,0x18,0x22,0x0e,
+ 0xff,0xe0,0xfb,0x3b,0x1e,0x00,0x00,0x18,0x02,0x00,0x00,0x00,0x18,0x00,0x00,
+ 0x00,0x00,0x18,0x04,0x00,0x00,0x00,0x18,0x06,0x00,0x00,0x00,0xfc,0x03,0x00};
--- /dev/null
+<HEAD>
+<TITLE>No Title</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html1 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif_gr.gif"> <IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif_gr.gif"> <A NAME=tex2html3 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html2 HREF=tableofcontents3_1.html>Contents</A>
+<HR> <P>
+<P>
+<b>
+<P>
+User Guide to
+<P>
+ALSCRIPT - Sequence alignment to PostScript<BR>Version 2.03<BR></b>
+<P>
+<em>Geoffrey J. Barton</em>
+<P>
+Laboratory of Molecular Biophysics<BR>University of Oxford<BR>Rex Richards Building<BR>South Parks Road<BR>Oxford OX1 3QU<BR>U.K.<BR>
+<P>
+Tel: (44) 1865-275368<BR>Fax: (44) 1865-510454<BR>e-mail: gjb@bioch.ox.ac.uk
+<P>
+REFERENCE:<BR>Barton, G. J. (1993),<BR>ALSCRIPT a tool to format multiple sequence alignments<BR>Protein Engineering, Volume 6, No. 1, pp.37-40.<BR>
+<P>
+<HR>
+<UL>
+<LI> <A NAME=tex2html4 HREF=tableofcontents3_1.html#SECTION0001000000000000000>Contents</A>
+<LI> <A NAME=tex2html5 HREF=section3_2.html#SECTION0002000000000000000> Update History</A>
+<LI> <A NAME=tex2html6 HREF=section3_3.html#SECTION0003000000000000000> Read This First - VERSION 2.0</A>
+<LI> <A NAME=tex2html7 HREF=section3_4.html#SECTION0004000000000000000> Related Programs</A>
+<LI> <A NAME=tex2html8 HREF=section3_5.html#SECTION0005000000000000000> Availability</A>
+<LI> <A NAME=tex2html9 HREF=section3_6.html#SECTION0006000000000000000> Installing ALSCRIPT</A>
+<LI> <A NAME=tex2html10 HREF=section3_7.html#SECTION0007000000000000000> Brief Description of ALSCRIPT</A>
+<LI> <A NAME=tex2html11 HREF=section3_8.html#SECTION0008000000000000000> New Features in Version 1.4.4</A>
+<LI> <A NAME=tex2html12 HREF=section3_9.html#SECTION0009000000000000000> New Features in Version 1.4.5 - Program alsnum</A>
+<LI> <A NAME=tex2html13 HREF=section3_10.html#SECTION00010000000000000000> New Features in Version 2.0</A>
+<UL>
+<LI> <A NAME=tex2html14 HREF=subsection3_10_1.html#SECTION00010100000000000000> New Step 1 Commands</A>
+<LI> <A NAME=tex2html15 HREF=subsection3_10_2.html#SECTION00010200000000000000> New Step 2 Commands</A>
+<LI> <A NAME=tex2html16 HREF=subsection3_10_3.html#SECTION00010300000000000000> New special TEXT commands</A>
+</UL>
+<LI> <A NAME=tex2html17 HREF=section3_11.html#SECTION00011000000000000000> Running ALSCRIPT</A>
+<UL>
+<LI> <A NAME=tex2html18 HREF=subsection3_11_1.html#SECTION00011100000000000000> Basic Use</A>
+<LI> <A NAME=tex2html19 HREF=subsection3_11_2.html#SECTION00011200000000000000> More complex effects - Text Lines, and Masks</A>
+</UL>
+<LI> <A NAME=tex2html20 HREF=section3_12.html#SECTION00012000000000000000> Using Colour</A>
+<LI> <A NAME=tex2html21 HREF=section3_13.html#SECTION00013000000000000000> The MASK command family</A>
+<UL>
+<LI> <A NAME=tex2html22 HREF=subsection3_13_1.html#SECTION00013100000000000000> Summary of mask commands</A>
+</UL>
+<LI> <A NAME=tex2html23 HREF=section3_14.html#SECTION00014000000000000000> Printing ALSCRIPT Files</A>
+<LI> <A NAME=tex2html24 HREF=section3_15.html#SECTION00015000000000000000> Conclusion</A>
+<LI> <A NAME=tex2html25 HREF=section3_16.html#SECTION00016000000000000000> Appendices</A>
+<UL>
+<LI> <A NAME=tex2html26 HREF=subsection3_16_1.html#SECTION00016100000000000000> ALSCRIPT Command Summary</A>
+<UL>
+<LI> <A NAME=tex2html27 HREF=subsubsection3_16_1_1.html#SECTION00016110000000000000> STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html28 HREF=subsubsection3_16_1_2.html#SECTION00016120000000000000> REQUIRED STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html29 HREF=subsubsection3_16_1_3.html#SECTION00016130000000000000> OPTIONAL STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html30 HREF=subsubsection3_16_1_4.html#SECTION00016140000000000000> STEP 2 COMMANDS</A>
+</UL>
+<LI> <A NAME=tex2html31 HREF=subsection3_16_2.html#SECTION00016200000000000000> AMPS Block file format</A>
+<LI> <A NAME=tex2html32 HREF=subsection3_16_3.html#SECTION00016300000000000000> PostScript Fonts</A>
+<LI> <A NAME=tex2html33 HREF=subsection3_16_4.html#SECTION00016400000000000000> 386 DOS installation</A>
+<LI> <A NAME=tex2html34 HREF=subsection3_16_5.html#SECTION00016500000000000000> TECHNICAL NOTES</A>
+<LI> <A NAME=tex2html35 HREF=subsection3_16_6.html#SECTION00016600000000000000> Unix Installation</A>
+<LI> <A NAME=tex2html36 HREF=subsection3_16_7.html#SECTION00016700000000000000> VAX/VMS Installation</A>
+<LI> <A NAME=tex2html37 HREF=subsection3_16_8.html#SECTION00016800000000000000> Alternative ways of invoking ALSCRIPT</A>
+<LI> <A NAME=tex2html38 HREF=subsection3_16_9.html#SECTION00016900000000000000> Program Crashes and Known Bugs</A>
+<LI> <A NAME=tex2html39 HREF=subsection3_16_10.html#SECTION000161000000000000000> Wish List for next version!!</A>
+<LI> <A NAME=tex2html40 HREF=subsection3_16_11.html#SECTION000161100000000000000> Acknowledgements</A>
+<LI> <A NAME=tex2html41 HREF=subsection3_16_12.html#SECTION000161200000000000000> References</A>
+</UL>
+<LI> <A NAME=tex2html42 HREF=section3_17.html#SECTION00017000000000000000> <em>About this document ...</em> </A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+# Associate symbolic labels with physical files.
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+<HEAD>
+<TITLE> New Features in Version 2.0</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html188 HREF=subsection3_10_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html186 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html180 HREF=section3_9.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html190 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html189 HREF=subsection3_10_1.html> New Step 1 </A>
+<B>Up:</B> <A NAME=tex2html187 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html181 HREF=section3_9.html> New Features in </A>
+<HR> <P>
+<H1><A NAME=SECTION00010000000000000000> New Features in Version 2.0</A></H1>
+<P>
+<A NAME=new2><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+Error checking is now done on all ranges input. If you run ALSCRIPT
+2.0 on a file that worked with ALSCRIPT 1.4.5, and it complains about
+out of range numbers, then check your ranges carefully. If you think
+you are right, then send me a minimal example of the problem and I
+will investigate. Versions of ALSCRIPT before 2.0 would often work
+happily with out of range numbers and produce perfectly OK output.
+<P>
+The files <b>ipns.als</b> and <b>ipns.blc</b> show example command and
+block file that use most of these new commands. See the <em>examples</em> directory.
+<P>
+<HR>
+<UL>
+<LI> <A NAME=tex2html191 HREF=subsection3_10_1.html#SECTION00010100000000000000> New Step 1 Commands</A>
+<LI> <A NAME=tex2html192 HREF=subsection3_10_2.html#SECTION00010200000000000000> New Step 2 Commands</A>
+<LI> <A NAME=tex2html193 HREF=subsection3_10_3.html#SECTION00010300000000000000> New special TEXT commands</A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Running ALSCRIPT</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html233 HREF=subsection3_11_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html231 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html225 HREF=subsection3_10_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html235 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html234 HREF=subsection3_11_1.html> Basic Use</A>
+<B>Up:</B> <A NAME=tex2html232 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html226 HREF=subsection3_10_3.html> New special TEXT </A>
+<HR> <P>
+<H1><A NAME=SECTION00011000000000000000> Running ALSCRIPT</A></H1>
+<P>
+<HR>
+<UL>
+<LI> <A NAME=tex2html236 HREF=subsection3_11_1.html#SECTION00011100000000000000> Basic Use</A>
+<LI> <A NAME=tex2html237 HREF=subsection3_11_2.html#SECTION00011200000000000000> More complex effects - Text Lines, and Masks</A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Using Colour</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html266 HREF=section3_13.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html264 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html258 HREF=subsection3_11_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html268 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html267 HREF=section3_13.html> The MASK command </A>
+<B>Up:</B> <A NAME=tex2html265 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html259 HREF=subsection3_11_2.html> More complex effects </A>
+<HR> <P>
+<H1><A NAME=SECTION00012000000000000000> Using Colour</A></H1>
+<P>
+Version 1.4 includes commands to allow the independent colouring of
+characters, or their backgrounds. Colours are defined in a similar
+manner to fonts using the DEFINE_COLOUR command (American spelling also
+allowed). For example:
+<P>
+DEFINE_COLOUR 7 1 0 0
+<P>
+defines colour number 7 to be red - see
+<A HREF=subsection3_16_1.html#app1>ALSCRIPT Command Summary</A>
+for full details of
+this command. Colours 99 and 100 are pre-defined to white and black.
+ALSCRIPT assumes the paper colour is white.
+<P>
+The command to colour the text of a character or text string is:
+<P>
+CCOL_CHARS
+<P>
+the command to colour the background of a character is
+<P>
+SCOL_CHARS
+<P>
+both have similar syntax to the FONT_CHARScommand.
+<P>
+COLOUR_REGION and COLOUR_RES have similar syntax to SHADE_REGION and
+SHADE_RES.
+<P>
+An example command file that uses colour is shown in example3.als.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> The MASK command family</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html277 HREF=subsection3_13_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html275 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html269 HREF=section3_12.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html279 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html278 HREF=subsection3_13_1.html> Summary of mask </A>
+<B>Up:</B> <A NAME=tex2html276 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html270 HREF=section3_12.html> Using Colour</A>
+<HR> <P>
+<H1><A NAME=SECTION00013000000000000000> The MASK command family</A></H1>
+<P>
+The idea behind the MASK command is to build up a set of character
+positions that will subsequently be boxed, shaded, set in a particular
+font, etc. For example, lets say we want to box the most frequently
+occuring character at each position in an alignment.
+<P>
+The command
+<P>
+mask SETUP
+<P>
+tells ALSCRIPT to prepare a mask.
+<P>
+mask FRE ALL
+<P>
+specifies that the most frequently occuring character at each position
+in the alignment will be masked. This command can be restricted to a
+region of the alignment using: mask FRE sx sy ex ey, where sx etc
+define the region in the same way as for font_region and other commands.
+<P>
+mask BOX ALL
+<P>
+Tells ALSCRIPT to create the boxing lines that will separate the masked
+characters from non-masked characters - this command may also be
+restricted to a region of the alignment.
+<P>
+The mask can be reset for re-use using the command:
+<P>
+mask RESET
+<P>
+Two further commands define which characters can be used when
+calculating the mask. This allows gap-characters, or other amino acids
+to be excluded from the calculation to avoid unwanted boxing.
+<P>
+mask LEGAL "AVLI"
+<P>
+defines the AVL and I as the only characters that will be used when
+calculating the mask.
+<P>
+mask ILLEGAL ".-_"
+<P>
+defines .- and _ as characters that will not be used when calculating
+the mask. NOTE: the blank character " " cannot be defined in this way.
+To avoid boxing " " characters substitute blanks for something else
+(using SUB_CHARS), calculate the mask, then substitute back.
+<P>
+<HR>
+<UL>
+<LI> <A NAME=tex2html280 HREF=subsection3_13_1.html#SECTION00013100000000000000> Summary of mask commands</A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Printing ALSCRIPT Files</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html298 HREF=section3_15.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html296 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html290 HREF=subsection3_13_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html300 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html299 HREF=section3_15.html> Conclusion</A>
+<B>Up:</B> <A NAME=tex2html297 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html291 HREF=subsection3_13_1.html> Summary of mask </A>
+<HR> <P>
+<H1><A NAME=SECTION00014000000000000000> Printing ALSCRIPT Files</A></H1>
+<P>
+ALSCRIPT produces files in PostScript which may be printed on a PostScript
+printer (e.g. an Apple LaserWriter). If you don't have a PostScript printer,
+then you may still be able to use ALSCRIPT if you get hold of the
+GhostScript software. This is a free package that interprets PostScript
+commands and can produce output on a large number of different types of
+printer. GhostScript runs on most hardware types (including PCs) and can also
+display output to the screen. The package can be obtained from many different
+sites on the Internet (In the UK try src.doc.ic.ac.uk).
+<P>
+The actual command you need to type to send a PostScript file to the printer
+will depend on your system. Consult your system manager for help.
+<P>
+Be warned, ALSCRIPT can create extremely large PostScript files if
+lots of boxing and shading is done on big alignments. On older
+printers such output may take a long time to process.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Conclusion</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html309 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html307 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html301 HREF=section3_14.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html311 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html310 HREF=section3_16.html> Appendices</A>
+<B>Up:</B> <A NAME=tex2html308 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html302 HREF=section3_14.html> Printing ALSCRIPT Files</A>
+<HR> <P>
+<H1><A NAME=SECTION00015000000000000000> Conclusion</A></H1>
+<P>
+ALSCRIPT provides a powerful set of formatting and editing commands
+specifically tailored for multiple sequence alignments. It is best
+used in conjunction with a PostScript previewer such as Sun's
+PageView or GhostView since this allows the effect of changing a command to be
+seen quickly. In the absence of such a tool, simpler effects can be
+tested out without destroying too many trees in the Laser Printer!
+<P>
+Like most programs, ALSCRIPT is evolving as I find new problems to display, so
+if you have any suggestions - I shall endeavour to include them in a later
+version.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Appendices</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html320 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html318 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html312 HREF=section3_15.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html322 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html321 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B>Up:</B> <A NAME=tex2html319 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html313 HREF=section3_15.html> Conclusion</A>
+<HR> <P>
+<H1><A NAME=SECTION00016000000000000000> Appendices</A></H1>
+<P>
+<HR>
+<UL>
+<LI> <A NAME=tex2html323 HREF=subsection3_16_1.html#SECTION00016100000000000000> ALSCRIPT Command Summary</A>
+<UL>
+<LI> <A NAME=tex2html324 HREF=subsubsection3_16_1_1.html#SECTION00016110000000000000> STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html325 HREF=subsubsection3_16_1_2.html#SECTION00016120000000000000> REQUIRED STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html326 HREF=subsubsection3_16_1_3.html#SECTION00016130000000000000> OPTIONAL STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html327 HREF=subsubsection3_16_1_4.html#SECTION00016140000000000000> STEP 2 COMMANDS</A>
+</UL>
+<LI> <A NAME=tex2html328 HREF=subsection3_16_2.html#SECTION00016200000000000000> AMPS Block file format</A>
+<LI> <A NAME=tex2html329 HREF=subsection3_16_3.html#SECTION00016300000000000000> PostScript Fonts</A>
+<LI> <A NAME=tex2html330 HREF=subsection3_16_4.html#SECTION00016400000000000000> 386 DOS installation</A>
+<LI> <A NAME=tex2html331 HREF=subsection3_16_5.html#SECTION00016500000000000000> TECHNICAL NOTES</A>
+<LI> <A NAME=tex2html332 HREF=subsection3_16_6.html#SECTION00016600000000000000> Unix Installation</A>
+<LI> <A NAME=tex2html333 HREF=subsection3_16_7.html#SECTION00016700000000000000> VAX/VMS Installation</A>
+<LI> <A NAME=tex2html334 HREF=subsection3_16_8.html#SECTION00016800000000000000> Alternative ways of invoking ALSCRIPT</A>
+<LI> <A NAME=tex2html335 HREF=subsection3_16_9.html#SECTION00016900000000000000> Program Crashes and Known Bugs</A>
+<LI> <A NAME=tex2html336 HREF=subsection3_16_10.html#SECTION000161000000000000000> Wish List for next version!!</A>
+<LI> <A NAME=tex2html337 HREF=subsection3_16_11.html#SECTION000161100000000000000> Acknowledgements</A>
+<LI> <A NAME=tex2html338 HREF=subsection3_16_12.html#SECTION000161200000000000000> References</A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> <em>About this document ...</em> </TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif_gr.gif"> <A NAME=tex2html519 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html515 HREF=subsection3_16_12.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html521 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B>Up:</B> <A NAME=tex2html520 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html516 HREF=subsection3_16_12.html> References</A>
+<HR> <P>
+<H1><A NAME=SECTION00017000000000000000> <em>About this document ...</em> </A></H1>
+<P>
+ This document was generated using the <A HREF=http://cbl.leeds.ac.uk/nikos/tex2html/doc/latex2html/latex2html.html><b>LaTeX</b>2<tt>HTML</tt></A> translator Version 0.5.3 (Wed Jan 26 1994) Copyright © 1993, <A HREF=http://cbl.leeds.ac.uk/nikos/personal.html>Nikos Drakos</A>, Computer Based Learning Unit, University of Leeds. <P> The command line arguments were: <BR>
+<b>latex2html</b> <tt>-address gjb@bioch.ox.ac.uk alscript.tex</tt>. <P>The translation was initiated by gjb@ on Wed Jun 5 16:43:14 BST 1996<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Update History</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html100 HREF=section3_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html98 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html92 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html102 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html101 HREF=section3_3.html> Read This First </A>
+<B>Up:</B> <A NAME=tex2html99 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html93 HREF=tableofcontents3_1.html>Contents</A>
+<HR> <P>
+<H1><A NAME=SECTION0002000000000000000> Update History</A></H1>
+<P>
+<PRE><TT>
+
+VERSION 1.0 19 June 1992
+Version 1.1 26 June 1992
+Version 1.2 21 October 1992: Add multiple blocks per page option.
+Version 1.3 15 November 1992: First Distribution.
+Version 1.4 6 December 1992: Add Colour commands.
+Version 1.4.1 1 February 1993: Small bug fixes - FULL RELEASE VERSION.
+Version 1.4.2 15 February 1993: Make silent_mode toggle.
+Version 1.4.3 1 March 1993: Fix bug in colour option.
+Version 1.4.4 24 March 1993: Add mask features (should be version 1.5).
+Version 1.4.5 25 May 1993: Include alsnum program in distribution.
+ 7 June 1993: Fix NO_NUMBERS bug in documentation
+ Change defaults for -q option to use MASK.
+
+Version 2.0 23 May 1995: Numerous changes and additions including the
+ option to colour backgrounds
+ differently, ommission of idents on
+ second and subsequent lines, helix,
+ strand and other special characters,
+ relative numbering, error checking of
+ ranges on input, bounding box,
+ screening, conservation colouring...
+Version 2.03 5 June 1996: Small bug fixes - patches incorporated.
+ BACKGROUND_REGION and BOUNDING_BOX commands moved to
+ step 1 section.</TT></PRE>
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Read This First - VERSION 2.0</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html111 HREF=section3_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html109 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html103 HREF=section3_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html113 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html112 HREF=section3_4.html> Related Programs</A>
+<B>Up:</B> <A NAME=tex2html110 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html104 HREF=section3_2.html> Update History</A>
+<HR> <P>
+<H1><A NAME=SECTION0003000000000000000> Read This First - VERSION 2.0</A></H1>
+<P>
+This manual describes an interim release of ALSCRIPT that includes
+many additional features over the previously distributed Version
+1.4.5. I had hoped to make a lot more changes and improvements before
+distributing the new version, however I have not had the time to do
+this. I am distributing Version 2.0 since the new features have been
+used in a number of published alignment figures. Please see
+<A HREF=section3_10.html#new2>New Features in Version 2.0</A> for details
+of the new features.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Related Programs</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html122 HREF=section3_5.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html120 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html114 HREF=section3_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html124 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html123 HREF=section3_5.html> Availability</A>
+<B>Up:</B> <A NAME=tex2html121 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html115 HREF=section3_3.html> Read This First </A>
+<HR> <P>
+<H1><A NAME=SECTION0004000000000000000> Related Programs</A></H1>
+<P>
+The AMPS package (Barton, 1990). This performs multiple sequence
+alignments and databank scanning.
+<P>
+AMAS (Livingstone and Barton, 1993, CABIOS, 9, 745-756). Analysis of
+Multiply Aligned Sequences. This package uses a sophisticated
+set-based method to identify patterns of residue conservation in
+multiple sequence alignments.
+<P>
+All programs are available by anonymous ftp from geoff.biop.ox.ac.uk.
+Please see the README file for details licencing and registration.
+You can read manuals for the programs and some related papers at
+http://geoff.biop.ox.ac.uk/.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Availability</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html133 HREF=section3_6.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html131 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html125 HREF=section3_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html135 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html134 HREF=section3_6.html> Installing ALSCRIPT</A>
+<B>Up:</B> <A NAME=tex2html132 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html126 HREF=section3_4.html> Related Programs</A>
+<HR> <P>
+<H1><A NAME=SECTION0005000000000000000> Availability</A></H1>
+<P>
+ALSCRIPT is available free of charge for academic and non-commercial
+purposes. Distribution is by anonymous ftp from geoff.biop.ox.ac.uk.
+See the README file on the ftp server for details. You need to
+register with G. J. Barton before downloading the software.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Installing ALSCRIPT</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html144 HREF=section3_7.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html142 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html136 HREF=section3_5.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html146 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html145 HREF=section3_7.html> Brief Description of </A>
+<B>Up:</B> <A NAME=tex2html143 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html137 HREF=section3_5.html> Availability</A>
+<HR> <P>
+<H1><A NAME=SECTION0006000000000000000> Installing ALSCRIPT</A></H1>
+<P>
+See the appropriate section for the computer type you are using:
+<P>
+For PCs see <A HREF=subsection3_16_4.html#app4>386 MS-DOS installation</A>.
+For Unix see <A HREF=subsection3_16_6.html#app5>Unix installation</A>.
+For VMS see <A HREF=subsection3_16_7.html#app6>VMS installation</A>.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Brief Description of ALSCRIPT</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html155 HREF=section3_8.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html153 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html147 HREF=section3_6.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html157 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html156 HREF=section3_8.html> New Features in </A>
+<B>Up:</B> <A NAME=tex2html154 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html148 HREF=section3_6.html> Installing ALSCRIPT</A>
+<HR> <P>
+<H1><A NAME=SECTION0007000000000000000> Brief Description of ALSCRIPT</A></H1>
+<P>
+ALSCRIPT takes a multiple sequence alignment in AMPS (Barton &Sternberg, 1987, Barton, 1990) block-file format and a set of
+formatting commands and produces a PostScript file that may be printed
+on a PostScript laser printer, or viewed using a PostScript previewer
+(e.g. Sun Microsystem's PageView program). CLUSTAL and GCG format
+multiple alignment files may also be used (see below). ALSCRIPT is NOT a
+multiple sequence alignment program, nor is it an alignment editor.
+<P>
+Given a block-file and pointsize (character width/height), ALSCRIPT
+calculates how many residues can be fitted across the page, and how
+many sequences will fit down the page, it then prints the alignment at
+the chosen pointsize on as many pages as are needed. Running ALSCRIPT with
+a smaller or larger pointsize will automatically re-scale the alignment
+to fit on fewer or more pages as appropriate. The actual page
+dimensions may be re-set to any value, so if you have access to an A3
+PostScript printer, or typesetting machine, alignments can readily be
+scaled to maximise the available space.
+<P>
+Each output page has three basic regions. The left hand edge contains
+identifier codes for each sequence. The main part of the page holds the
+alignment, and the top part, the position numbers and tick marks. ALSCRIPT
+commands make use of a character coordinate system for font changes,
+and other formatting commands. Thus, any residue in the alignment may
+be referred to by its sequence position number (x-axis) and sequence
+number (y-axis), similarly, ranges of residue positions, or sequences
+may also be defined in the character coordinate system.
+<P>
+The basic ALSCRIPT commands allow the following functionality:
+<P>
+Fonts: Any PostScript font at any size may be defined and used on
+individual residues, regions or identifier codes.
+<P>
+Boxing: Simple rectangular boxes may be drawn around any part of the
+alignment. Particular residue types may be selected and automatically
+"surrounded" by lines. For example, if the characters 'G' and 'P' are
+selected, then lines will not be drawn between G and P characters, but
+only where G and P border with other characters.
+<P>
+Shading: Grey shading of any level from black to white may be applied
+to any region of the alignment, either as a rectangular region, or as
+residue specific shading. e.g. "shade all Cys residues between
+positions 6 and 30"
+<P>
+Text: Specific text strings may be added to the alignment at any
+position and in any font or font size.
+<P>
+Lines: Horizontal or vertical lines may be drawn to the left, right, top
+or bottom of any residue position or group of positions.
+<P>
+Colour: Characters or character backgrounds may be independently
+coloured.
+<P>
+The example block file "example1.blc" and command file "example1.als"
+illustrate most of these commands in action.
+<P>
+Although written with the aim of producing figures for journal
+submission, ALSCRIPT may be used as a tool for interpreting multiple
+sequence alignments. For example, the boxing, shading and font changing
+facilities can be applied to highlight amino acids of a particular type
+and thus draw attention to clusters of positive or negative charge,
+hydrophobics, etc.
+<P>
+<HR> <A NAME=tex2html155 HREF=section3_8.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html153 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html147 HREF=section3_6.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html157 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html156 HREF=section3_8.html> New Features in </A>
+<B>Up:</B> <A NAME=tex2html154 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html148 HREF=section3_6.html> Installing ALSCRIPT</A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> New Features in Version 1.4.4</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html166 HREF=section3_9.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html164 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html158 HREF=section3_7.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html168 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html167 HREF=section3_9.html> New Features in </A>
+<B>Up:</B> <A NAME=tex2html165 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html159 HREF=section3_7.html> Brief Description of </A>
+<HR> <P>
+<H1><A NAME=SECTION0008000000000000000> New Features in Version 1.4.4</A></H1>
+<P>
+This version introduces the MASK family of commands which allows
+boxing, shading etc to be applied according to the frequency of
+occurence of the character types at each position in the alignment.
+For example, it is possible to box positions where one character is
+seen in more than N of the sequences. It is also possible to
+box/shade etc the most frequently occurring character at each
+position. Commands exist to select which characters will be used in
+the calculation of frequencies and which will be excluded, thus boxing
+can be based upon two or more character types at a position. MASK
+commands also exist to show residues identical to one sequence in the
+set. See the section on MASK below for details.
+<P>
+NOTE: Although boxing according to the frequency of amino acids seen
+at a position is a popular method of representation it is not usually
+the most informative. An analysis that takes into account the
+physico-chemical properties of the amino acids and also relates the
+amino acid similarities to the overall similarity between the
+sequences is more helpful in identifying functionally important
+residues. The AMAS program (Livingstone and Barton, 1993) applies a
+flexible hierarchical set-based approach to this problem.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> New Features in Version 1.4.5 - Program alsnum</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html177 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html175 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html169 HREF=section3_8.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html179 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html178 HREF=section3_10.html> New Features in </A>
+<B>Up:</B> <A NAME=tex2html176 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html170 HREF=section3_8.html> New Features in </A>
+<HR> <P>
+<H1><A NAME=SECTION0009000000000000000> New Features in Version 1.4.5 - Program alsnum</A></H1>
+<P>
+Version 1.4.5 includes the program "alsnum". This is a temporary solution to
+the residue numbering problem. Ultimately, these functions will be included
+as alscript commands.
+<P>
+alsnum creates a set of TEXT commands that can be incorporated into an
+alscript command file to place sequential numbers at any position in the
+alignment. The numbers ignore gaps, so the numbering will correspond to
+the specific sequence position rather than the alignment.
+<P>
+To use the program:
+<P>
+1. Decide where you want the numbers to be placed. For example, you might
+want the numbers above the third sequence in the alignment. If so, make
+an extra sequence space above the third sequence using the ADD_SEQ command.
+<P>
+2. Decide what is the number of the first residue of the sequence to be
+numbered. This will not always be 1 since you may be aligning fragments
+or domains.
+<P>
+3. Decide the numbering interval (e.g. every 10th amino acid).
+<P>
+4. Run the program.
+<P>
+For example, if you want to add numbers according to sequence 37 of a
+block file (junk.blc), calling the first residue of the sequence 12,
+and with an interval of 5, and the numbers are to be placed at the
+location of sequence 3 in the alignment. Type:
+<P>
+alsnum 37 12 5 3 < junk.blc > junk.text
+<P>
+5. Add the resulting TEXT commands from junk.text to your alscript
+command file.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> New Step 1 Commands</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html202 HREF=subsection3_10_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html200 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html194 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html204 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html203 HREF=subsection3_10_2.html> New Step 2 </A>
+<B>Up:</B> <A NAME=tex2html201 HREF=section3_10.html> New Features in </A>
+<B> Previous:</B> <A NAME=tex2html195 HREF=section3_10.html> New Features in </A>
+<HR> <P>
+<H2><A NAME=SECTION00010100000000000000> New Step 1 Commands</A></H2>
+<P>
+SCREENSIZE 120
+<P>
+Usually you should not need to change this value, it alters the
+screening used by the printer. A value of 120 is used by default. On
+most 300dpi black and white printers this gives much smoother greys
+than the default used in earlier versions of ALSCRIPT.
+<P>
+PIR_SAVE filename
+<P>
+Will cause the block file to be saved into the file ``filename'' in
+PIR format. This can be useful for moving block file alignments to other
+programs.
+<P>
+MSF_SAVE filename
+<P>
+Will cause the block file to be saved into the file ``filename'' in
+something that approximates GCG .msf format. <em>Warning! This has not
+been fully tested.</em>
+<P>
+NUMBER_COLOUR 4
+<P>
+Sets the colour used for numbering at the top of the alignment (no
+American spelling at the moment). In this example, colour number 4 has been
+defined (See the DEFINE_COLOURcommand if you are not sure what this means).
+<P>
+SINGLE_PAGEIf this is set, ALSCRIPT assumes everything will be plotted on one
+page. At the moment, all this does is write the bounding box for the
+figure, so encapsulating the PostScript. This <em>may</em> allow the
+output of alscript to be imported into word processors etc, but
+probably not all.
+<P>
+ID_ONLY_ON_FIRST_LINEIf this is present, then sequence identifiers will only be printed on
+the first line of the alignment. Often this looks better for small
+alignments than the default.
+<P>
+BACKGROUND_COLOUR 7
+<P>
+Sets the colour used for the background to the alignment. This can be
+useful for preparing figures for projection. At the moment this only
+works reliably when the SINGLE_PAGEis also set.
+<P>
+BOUNDING_BOX x y x1 y1
+<P>
+Defines the bounding box for the figure. This is set in points (1/72 inch).
+NOTE: In version 2.0 this was a STEP 2 Command.
+<P>
+BACKGROUND_REGION x y x1 y1
+<P>
+Defines the region to colour as background - the default is set up for A4 paper
+so US users may have to fiddle with this. Values are points (1/72 inch).
+NOTE: In version 2.0 this was a STEP 2 Command.
+<P>
+<HR> <A NAME=tex2html202 HREF=subsection3_10_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html200 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html194 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html204 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html203 HREF=subsection3_10_2.html> New Step 2 </A>
+<B>Up:</B> <A NAME=tex2html201 HREF=section3_10.html> New Features in </A>
+<B> Previous:</B> <A NAME=tex2html195 HREF=section3_10.html> New Features in </A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> New Step 2 Commands</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html213 HREF=subsection3_10_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html211 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html205 HREF=subsection3_10_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html215 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html214 HREF=subsection3_10_3.html> New special TEXT </A>
+<B>Up:</B> <A NAME=tex2html212 HREF=section3_10.html> New Features in </A>
+<B> Previous:</B> <A NAME=tex2html206 HREF=subsection3_10_1.html> New Step 1 </A>
+<HR> <P>
+<H2><A NAME=SECTION00010200000000000000> New Step 2 Commands</A></H2>
+<P>
+COLOUR_TEXT_REGION x y x1 y1 colour
+<P>
+Sets the colour for TEXT command output. Similar syntax to COLOUR_REGION, FONT_REGIONetc.
+<P>
+COLOUR_LINE_REGION x y x1 y1 colour
+<P>
+Set the colour for LINEs in a region.
+<P>
+CALCONS x y x1 y1
+<P>
+Calculate conservation values according to Zvelebil <em>et al.</em> for the designated
+region. (See Livingstone &Barton 1993 for details and further refs)
+<P>
+MASK CONSERVATION cutoff
+<P>
+If CONSCAL has been used, then mask residues according to the conservation cutoff.
+<P>
+e.g. MASK CONSERVATION 10 would mask all identities, MASK CONSERVATION 6 would
+mask reasonably conserved positions. See examples for more on this command.
+<P>
+HELIX x1 y x2
+<P>
+Draw a helix from x1 to x2 of sequence y.
+<P>
+STRAND x1 y x2
+<P>
+Draw a strand from x1 to x2 of sequence y.
+<P>
+COIL x1 y x2
+<P>
+Draw a coil (horizontal line) from x1 to x2 of sequence y.
+<P>
+RELATIVE_TO<seqnum> <startnum>
+<P>
+Set reference numbers to work relative to sequence number <seqnum>. This
+means that in all subsequent commands, ALSCRIPT will translate your x
+values into absolute position values in the alignment. This is <em>extremely</em> useful since you can annotate your alignment using your
+favourite sequences as a reference point. You no longer have to
+translate every x position into the alignment position.
+<P>
+<startnum> is optional. If present, it specifies what the first
+residue in the displayed sequence is. For example, you may be showing
+residues 200-500 of a sequence, so <startnum> would be 200 rather than
+the default of 1. <em>Warning - this is a very new feature and
+bounds checking is not fully enabled for it</em>.
+<P>
+You can use RELATIVE_TOseveral times in the command file to annotate
+different sequences. RELATIVE_TO0 resets to the ``normal''
+alignment numbering.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> New special TEXT commands</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html222 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html220 HREF=section3_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html216 HREF=subsection3_10_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html224 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html223 HREF=section3_11.html> Running ALSCRIPT</A>
+<B>Up:</B> <A NAME=tex2html221 HREF=section3_10.html> New Features in </A>
+<B> Previous:</B> <A NAME=tex2html217 HREF=subsection3_10_2.html> New Step 2 </A>
+<HR> <P>
+<H2><A NAME=SECTION00010300000000000000> New special TEXT commands</A></H2>
+<P>
+Some special TEXT commands have been added to allow drawing of alternative
+shapes etc. In fact this is how the HELIX, STRAND and COIL commands are implemented. The text commands are all prefixed by an @ symbol.
+<P>
+e.g. TEXT 3 6 ``@fuparrow''
+<P>
+will draw a filled up arrow at position 3,6.
+<P>
+The alternative text commands are:
+<P>
+@leftarrow - an open left pointing arrow.
+<P>
+@fleftarrow - a filled left pointing arrow.
+<P>
+@uparrow - an open up pointing arrow.
+<P>
+@fuparrow - a filled up pointing arrow.
+<P>
+@downarrow - an open down pointing arrow.
+<P>
+@fdownarrow - a filled down pointing arrow.
+<P>
+@circle - an open circle.
+<P>
+@fcircle - a filled circle.
+<P>
+I plan to make this option more flexible in the near future.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Basic Use</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html246 HREF=subsection3_11_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html244 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html238 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html248 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html247 HREF=subsection3_11_2.html> More complex effects </A>
+<B>Up:</B> <A NAME=tex2html245 HREF=section3_11.html> Running ALSCRIPT</A>
+<B> Previous:</B> <A NAME=tex2html239 HREF=section3_11.html> Running ALSCRIPT</A>
+<HR> <P>
+<H2><A NAME=SECTION00011100000000000000> Basic Use</A></H2>
+<P>
+I recommend you read through this section, then scan the commands in
+<A HREF=subsection3_16_1.html#app1>ALSCRIPT Command Summary</A> to get a feel for what ALSCRIPT can do.
+<P>
+See <A HREF=subsection3_16_8.html#app7>Alternative ways of invoking ALSCRIPT</A>. In this section, the
+interactive method is described. The QUICK START method shown in
+<A HREF=subsection3_16_8.html#app7>Alternative ways of invoking ALSCRIPT</A>
+is useful to format a sequence alignment quickly
+using standard pointsize and shading.
+<P>
+ALSCRIPT is designed to work with AMPS block file format multiple
+alignments. If you have a multiple alignment generated by CLUSTAL V
+or the GCG package, then it must be translated to AMPS block file
+format.
+<P>
+To translate a GCG .MSF file: Type: msf2blc.
+To translate a CLUSTAL PIR format file, or any PIR format file: clus2blc.
+<P>
+Both programs prompt for the name of an input file, and an output
+block file name. A good convention to follow is to name all blockfiles
+with the extension ".blc".
+<P>
+To run ALSCRIPT simply type:
+<P>
+alscript
+<P>
+you will then be prompted for the name of the ALSCRIPT command file.
+Having typed the filename, the commands will be executed as you have
+specified.
+<P>
+A Simple Command File (example.als)
+<P>
+The file example.blc contains a small multiple sequence alignment.
+The following ALSCRIPT command file will convert this into a
+PostScript alignment file in 12 point Helvetica.
+<P>
+<PRE><TT>
+#Comments in ALSCRIPT command files start with a #
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+SETUP #Tell the program to get on with it.</TT></PRE>
+<P>
+Now try changing the POINTSIZE value to 5 ALSCRIPT will re-format the
+alignment to make best use of the available paper.
+<P>
+These are all STEP 1 commands - they refer to overall layout, and system
+settings - for example, the paper size or maximum sequence length.
+Other commonly used STEP 1 commands are IDENT_WIDTH which reserves more
+or less width for the sequence identifier codes, NUMBER_SEQS which adds
+a number to each sequence and LINE_WIDTH_FACTOR which allows the
+thickness of all boxing lines to be adjusted. See
+<A HREF=subsection3_16_1.html#app1>ALSCRIPT Command Summary</A>
+for details of these and all other STEP 1 commands.
+<P>
+The simple example outlined above can be modified with a variety of
+STEP 2 commands.
+<P>
+for example file example2.als:
+<P>
+<PRE><TT>
+# FILE example2.als
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example2.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica
+DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic
+NUMBER_SEQS #Number the sequences at left hand side
+SETUP #Tell the program to get on with it.
+#
+#step 2 commands come after the SETUP command
+#
+#Here are some examples...
+#
+SURROUND_CHARS GP ALL #draw lines around all G and P
+SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6
+BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20
+FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters
+ID_FONT ALL 1 #set identifiers in font 1</TT></PRE>
+<P>
+There are many possible ways of combining these commands and the
+others shown in
+<A HREF=subsection3_16_1.html#app1>ALSCRIPT Command Summary</A>.
+In general, if you apply multiple
+commands to the same residue, the effect of the last applied command
+persists where there would otherwise be a conflict. Thus the
+intersection of two overlapping SHADed regions would be shaded
+according to the second SHADE command, not some mixture of the two.
+Similarly for FONT commands. BOX and SURROUND commands behave in the
+opposite sense, all BOXing and SURROUNDing persists regardless of how
+many commands you issue. This makes it possible for example, to
+SURROUND two different sets of residues as follows:
+<P>
+<PRE><TT>
+
+SURROUND_CHARS DE ALL
+SURROUND_CHARS DEHKR ALL</TT></PRE>
+<P>
+This would result in D and E characters being partitioned from the rest as well
+as D E H K R characters (see Example output).
+<P>
+<HR> <A NAME=tex2html246 HREF=subsection3_11_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html244 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html238 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html248 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html247 HREF=subsection3_11_2.html> More complex effects </A>
+<B>Up:</B> <A NAME=tex2html245 HREF=section3_11.html> Running ALSCRIPT</A>
+<B> Previous:</B> <A NAME=tex2html239 HREF=section3_11.html> Running ALSCRIPT</A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> More complex effects - Text Lines, and Masks</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html255 HREF=section3_12.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html253 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html249 HREF=subsection3_11_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html257 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html256 HREF=section3_12.html> Using Colour</A>
+<B>Up:</B> <A NAME=tex2html254 HREF=section3_11.html> Running ALSCRIPT</A>
+<B> Previous:</B> <A NAME=tex2html250 HREF=subsection3_11_1.html> Basic Use</A>
+<HR> <P>
+<H2><A NAME=SECTION00011200000000000000> More complex effects - Text Lines, and Masks</A></H2>
+<P>
+Text, lines and masking are meant to be used to annotate the multiple
+alignment. The TEXT command allows any piece of text to be located
+anywhere on the alignment. Clearly, however it makes little sense to
+superimpose the text over the alignment though this can be done! Accordingly,
+you must first make a space to put the text in. Usually, this will be a
+few lines below the multiple sequence alignment, but you may want to
+add text at the top, or somewhere in between two sequences. You can make
+space in two ways. Either by editing the block-file to introduce "dummy"
+sequences at the locations you want, or by making use of the ADD_SEQ
+command.
+<P>
+The ADD_SEQ command has two arguments, the sequence after which you want
+further sequences to be added, and how many blank sequences you need.
+Thus, we can reserve space for 5 lines of text underneath a 10 sequence
+multiple alignment with the following command.
+<P>
+ADD_SEQ 10 5
+<P>
+we can then put text below the alignment at the 20th residue.
+<P>
+TEXT 20 13 "Active Site His"
+<P>
+or any other position.
+<P>
+Similarly, we could draw a vertical line to point out which residue we mean
+<P>
+LINE LEFT 20 13 11
+<P>
+And change the font of the text to number 7 (whatever that has been set to):
+<P>
+FONT_RESIDUE 20 13 7
+<P>
+You can have multiple ADD_SEQ commands, but they must occur in sequence order.
+Thus:
+<P>
+<PRE><TT>
+ADD_SEQ 0 5
+ADD_SEQ 5 12</TT></PRE>
+is legal.
+But
+<PRE><TT>
+ADD_SEQ 5 12
+ADD_SEQ 0 5</TT></PRE>
+<P>
+Is NOT!! NO CHECKING IS performed by the program for this error - so beware!
+<P>
+Note that add_seq commands refer to the actual sequence number as
+implied by the block file, not the number after applying the add_seq
+command. Thus, for a four sequence block file, if you want to add
+space for three sequences before sequence 1 and two sequences after
+sequence 3, the commands would be:
+<P>
+<PRE><TT>
+ADD_SEQ 0 3
+ADD_SEQ 3 2</TT></PRE>
+<P>
+Text added with the TEXT command will not be split across page breaks,
+so you may in some circumstances need to fiddle a little with the
+location/pointsize for the text to get the desired result.
+<P>
+Masking is a technique for drawing irregular shaped outlines, or
+shaded regions - this should not be confused with the MASK family of commands
+described below. For example a histogram can be added to the bottom of
+an alignment by first defining some dummy sequences in the block-file
+that have letters building up the shape of the histogram, then using
+the SURROUND_CHARS or SHADE_CHARS commands together with the SUB_CHARS
+command to produce the desired effect. An example of this operation
+being used to show frequencies of secondary structure predictions is
+shown in example1.als and in the Protein Engineering paper.
+<P>
+<HR> <A NAME=tex2html255 HREF=section3_12.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html253 HREF=section3_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html249 HREF=subsection3_11_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html257 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html256 HREF=section3_12.html> Using Colour</A>
+<B>Up:</B> <A NAME=tex2html254 HREF=section3_11.html> Running ALSCRIPT</A>
+<B> Previous:</B> <A NAME=tex2html250 HREF=subsection3_11_1.html> Basic Use</A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Summary of mask commands</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html287 HREF=section3_14.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html285 HREF=section3_13.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html281 HREF=section3_13.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html289 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html288 HREF=section3_14.html> Printing ALSCRIPT Files</A>
+<B>Up:</B> <A NAME=tex2html286 HREF=section3_13.html> The MASK command </A>
+<B> Previous:</B> <A NAME=tex2html282 HREF=section3_13.html> The MASK command </A>
+<HR> <P>
+<H2><A NAME=SECTION00013100000000000000> Summary of mask commands</A></H2>
+<P>
+mask SETUP
+#Prepares for masking
+<P>
+mask LEGAL <qstring>
+#defines characters to include in ID or FRE calcs - optional.
+<P>
+mask ILLEGAL <qstring>
+#defines characters to exclude in ID or FRE calcs - optional.
+<P>
+mask ID ALL N
+#Calculates a mask that flags the character that occurs at least N
+times at a position. The word ALL can be substituted by four numbers
+that define a region of the alignment.
+<P>
+mask FRE ALL
+#calculates a mask that flags the most frequently occuring amino acid
+at each position. ALL may be replaced by four numbers defining a region
+of the alignment.
+<P>
+Multiple mask FRE or mask ID commands may be applied, using different
+LEGAL and ILLEGAL character definitions. In this way more complex
+effects can be built up.
+<P>
+The mask command also allows characters that are identical to one
+sequence to be masked.
+<P>
+mask AGREE ALL N
+<P>
+will mask all positions that are identical to the Nth sequence. Thus,
+for sequences that are very similar to a newly sequenced sequence, all
+characters identical to the new sequence can be boxed or shaded, or set
+in a different font or colour etc...
+<P>
+mask NOT ALL
+<P>
+allows the mask to be inverted. Thus, all positions that are NOT in the
+mask now form the mask. So, having done a mask AGREE, a mask NOT will
+allow the positions that are not identical to the selected sequence to
+be highlighted or substituted.
+<P>
+mask SUB ALL <char>
+<P>
+substitutes all characters in the mask with the character <char>.
+<P>
+mask REGION ALL
+applies a mask to all residues in the defined region.
+<P>
+The following effects can now be applied to the masked characters:
+<P>
+mask BOX ALL
+#boxes the masked residues - ie surrounds them by lines.
+<P>
+mask SHADE ALL <grey>
+#shades the masked residues by grey value.
+<P>
+mask FONT ALL <fontnum>
+#Uses font fontnum to output the masked residues.
+<P>
+mask INVERSE ALL
+#Inverts the masked characters - ie outputs them in white.
+<P>
+mask CCOL ALL <colnum>
+#outputs the masked characters in the defined colour.
+<P>
+mask SCOL ALL <colnum>
+#outputs the backgrounds of the masked characters in the defined
+colour.
+<P>
+In all commands, the word ALL can be replaced by four numbers defining
+the region to which the command is applied.
+<P>
+mask RESET
+#resets the entire mask for re-use
+<P>
+<HR> <A NAME=tex2html287 HREF=section3_14.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html285 HREF=section3_13.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html281 HREF=section3_13.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html289 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html288 HREF=section3_14.html> Printing ALSCRIPT Files</A>
+<B>Up:</B> <A NAME=tex2html286 HREF=section3_13.html> The MASK command </A>
+<B> Previous:</B> <A NAME=tex2html282 HREF=section3_13.html> The MASK command </A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> ALSCRIPT Command Summary</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html347 HREF=subsubsection3_16_1_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html345 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html339 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html349 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html348 HREF=subsubsection3_16_1_1.html> STEP 1 COMMANDS</A>
+<B>Up:</B> <A NAME=tex2html346 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html340 HREF=section3_16.html> Appendices</A>
+<HR> <P>
+<H2><A NAME=SECTION00016100000000000000> ALSCRIPT Command Summary</A></H2>
+<P>
+<A NAME=app1><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+WARNING: Very little error checking is performed on command input. If
+you give the wrong number of arguments to a command, then unexpected
+things may happen, or the program will crash very inelegantly. I hope
+to fix this in the next version of the program, in the meantime, make sure
+you give the correct number of arguments to each command.
+<P>
+All commands up to the first space character may be entered in UPPER
+or lower case or MiXEd case. Qualifiers for commands (e.g. REL) must
+be written in UPPER case.
+<P>
+Command Reference:
+<P>
+<PRE><TT>
+<int> = enter an integer (e.g. 240)
+<float> = enter a floating point number (e.g. 0.45)
+<string> = enter a string (e.g. ARNDql)
+<qstring> = enter a quoted string (e.g. "Active Site")
+<char> = enter a single character.</TT></PRE>
+<P>
+<HR>
+<UL>
+<LI> <A NAME=tex2html350 HREF=subsubsection3_16_1_1.html#SECTION00016110000000000000> STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html351 HREF=subsubsection3_16_1_2.html#SECTION00016120000000000000> REQUIRED STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html352 HREF=subsubsection3_16_1_3.html#SECTION00016130000000000000> OPTIONAL STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html353 HREF=subsubsection3_16_1_4.html#SECTION00016140000000000000> STEP 2 COMMANDS</A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Wish List for next version!!</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html492 HREF=subsection3_16_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html490 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html484 HREF=subsection3_16_9.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html494 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html493 HREF=subsection3_16_11.html> Acknowledgements</A>
+<B>Up:</B> <A NAME=tex2html491 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html485 HREF=subsection3_16_9.html> Program Crashes and </A>
+<HR> <P>
+<H2><A NAME=SECTION000161000000000000000> Wish List for next version!!</A></H2>
+<P>
+<A NAME=app9><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+A command interpreter that does more error checking will be included.
+Currently, no checking is done to make sure that the correct number of
+arguments are given to a command.
+<P>
+Sequences will be able to be given unique labels and region commands refer
+to these labels or ranges of labels. This will permit a sequence to be
+deleted or added to the alignment without having to update the .als file.
+<P>
+The relative numbering option will be extended to allow numbering relative
+to a position. e.g. 456+7 would be 7 residues after position 456. This
+will allow annotation of positions that may be in insertions relative to the
+reference sequence.
+<P>
+Special TEXT commands will be extended to allow alternative shapes to
+be drawn and scaled in various ways.
+<P>
+Tree drawing and generalised graphics. An option to draw arbitrary lines
+on an alignment will be added. This will permit line graphics to be added
+to an alignment. The initial reason for this will be to show dendrograms
+(trees) alongside the alignment, but simple line graphs could also be plotted
+under the alignment.
+<P>
+Fiddle factors will be introduced to allow fine positioning of
+individual characters. For example, if you like your ``I'' characters
+to be centred rather than left justified, this will be possible.
+<P>
+In single_pagemode, it will be possible to add arbitrary text to an
+alignment for final annotation, e.g. titles etc.
+<P>
+Variable height/width sequence lines will be permitted (maybe).
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Acknowledgements</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html503 HREF=subsection3_16_12.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html501 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html495 HREF=subsection3_16_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html505 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html504 HREF=subsection3_16_12.html> References</A>
+<B>Up:</B> <A NAME=tex2html502 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html496 HREF=subsection3_16_10.html> Wish List for </A>
+<HR> <P>
+<H2><A NAME=SECTION000161100000000000000> Acknowledgements</A></H2>
+<P>
+I thank all those who have emailed me with suggestions for
+improvements to alscript. I've tried to include some of these in the
+current distribution (e.g. screening).
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> References</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html512 HREF=section3_17.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html510 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html506 HREF=subsection3_16_11.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html514 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html513 HREF=section3_17.html> About this document </A>
+<B>Up:</B> <A NAME=tex2html511 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html507 HREF=subsection3_16_11.html> Acknowledgements</A>
+<HR> <P>
+<H2><A NAME=SECTION000161200000000000000> References</A></H2>
+<P>
+<A NAME=app10><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+<PRE><TT>
+
+1. Barton, G. J. (1993),
+ "ALSCRIPT A tool to format multiple sequence alignments",
+ Protein Engineering, Volume 6, No. 1, pp.37-40.
+
+2. Barton, G. J. (1990),
+ "Protein Multiple Sequence Alignment and Flexible Pattern Matching",
+ Methods in Enzymology,
+ 183,403-428.
+
+3. Barton, G. J. and Sternberg, M. J. E. (1987),
+ "A Strategy for the Rapid Multiple Alignment of Protein Sequences:
+ Confidence Levels From Tertiary Structure Comparisons",
+ Journal of Molecular Biology,
+ 198,327-337
+
+4. Higgins, D. G. and Sharp, P. M. (1989),
+ "Fast and sensitive multiple sequence alignments on a microcomputer",
+ CABIOS,
+ 5,151--153
+
+5. Devereux, J. Haeberli, P. Smithies, O. (1984),
+ "A comprehensive set of sequence analysis programs for the VAX",
+ Nucleic Acids Res.
+ 12, 387-395
+
+6. Livingstone, C. D. and Barton, G. J. (1993),
+ "Protein Sequence Alignments: A Strategy for the Hierarchical analysis
+ of residue conservation"
+ Computer Applications in the Biosciences,
+ 9, 745-756.</TT></PRE>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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--- /dev/null
+<HEAD>
+<TITLE> AMPS Block file format</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html404 HREF=subsection3_16_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html402 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html396 HREF=subsubsection3_16_1_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html406 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html405 HREF=subsection3_16_3.html> PostScript Fonts</A>
+<B>Up:</B> <A NAME=tex2html403 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html397 HREF=subsubsection3_16_1_4.html> STEP 2 COMMANDS</A>
+<HR> <P>
+<H2><A NAME=SECTION00016200000000000000> AMPS Block file format</A></H2>
+<P>
+<A NAME=app2><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+The first part of a block-file contains the identifier codes of the
+sequences that are to follow. Each code is prefixed by the > symbol, codes
+must not contain spaces.
+<P>
+e.g.
+<PRE><TT>
+>HAHU
+>Trypsin
+>A0046
+>Seq1</TT></PRE>
+<P>
+etc.
+<P>
+ALSCRIPT counts the number of > symbols in the beginning of the file
+until a * symbol is found. The * signals the beginning of the
+multiple alignment which is stored VERTICALLY, thus columns are
+individual sequences, whilst rows are aligned positions. The * symbol
+must lie over the first sequence. A further star in the same column
+signals the end of the alignment. ALSCRIPT uses the number of >
+symbols at the beginning of the file to work out how many columns to
+read from the * position. It is therefore important that the only >
+symbols in the file are those that define the identifiers, and the
+only * symbols are those defining the start and end of the multiple
+alignment. The block file can contain additional text, providing that
+there are no more > or * symbols in the file than those used to define
+the identifiers or alignment start and end.
+<P>
+A simple, small block-file is shown here.
+<P>
+<PRE><TT>
+>Seq_1
+>A0231
+>HAHU
+>Four_Alpha
+>Globin
+>GLobin_C
+*
+ARNDLQ
+AAAAAA
+PPPPPP
+PP PPP
+WW WWW
+LLLLLL
+IIVVLL
+*</TT></PRE>
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> PostScript Fonts</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html415 HREF=subsection3_16_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html413 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html407 HREF=subsection3_16_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html417 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html416 HREF=subsection3_16_4.html> 386 DOS installation</A>
+<B>Up:</B> <A NAME=tex2html414 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html408 HREF=subsection3_16_2.html> AMPS Block file </A>
+<HR> <P>
+<H2><A NAME=SECTION00016300000000000000> PostScript Fonts</A></H2>
+<P>
+<A NAME=app3><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<PRE><TT>
+Times-Roman,
+Times-Italic,
+Times-Bold,
+Times-BoldItalic,
+Helvetica,
+Helvetica-Oblique,
+Helvetica-Bold,
+Helvetica-BoldOblique,
+Courier,
+Courier-Oblique,
+Courier-Bold
+Courier-BoldOblique,
+AvantGarde-Book,
+AvantGarde-BookOblique,
+AvantGarde-Demi,
+AvantGarde-DemiOblique,
+Bookman-Demi,
+Bookman-DemiItalic,
+Bookman-Light,
+Bookman-LightItalic,
+Helvetica-Narrow,
+Helvetica-Narrow-Bold,
+Helvetica-Narrow-BoldOblique,
+Helvetica-Narrow-Oblique,
+NewCenturySchblk-Roman,
+NewCenturySchlbk-Bold,
+NewCenturySchblk-Italic,
+NewCenturySchblk-BoldItalic,
+Palatino-Roman,
+Palatino-Bold,
+Palatino-Italic,
+Palatino-BoldItalic
+ZapfChancery-MediumItalic.
+Symbol</TT></PRE>
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> 386 DOS installation</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html426 HREF=subsection3_16_5.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html424 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html418 HREF=subsection3_16_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html428 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html427 HREF=subsection3_16_5.html> TECHNICAL NOTES</A>
+<B>Up:</B> <A NAME=tex2html425 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html419 HREF=subsection3_16_3.html> PostScript Fonts</A>
+<HR> <P>
+<H2><A NAME=SECTION00016400000000000000> 386 DOS installation</A></H2>
+<P>
+<A NAME=app4><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+IMPORTANT - The programs on this disk will ONLY WORK on a PC with a 386
+or better processor. See the Technical Notes section for details of why.
+<P>
+Directions:
+<P>
+<OL><LI>
+ Create a directory on your hard disk.
+ e.g. mkdir ALSCRIPT.
+<P>
+<LI>
+ Copy the Contents of the floppy disk into this directory.
+<PRE><TT>
+ e.g. copy a:*.* c:\alscript.</TT></PRE>
+<P>
+<LI>
+ Edit your AUTOEXEC.BAT file and add
+<PRE><TT>
+ C:\ALSCRIPT to your path.</TT></PRE>
+<P>
+<LI>
+ Edit your AUTOEXEC.BAT file and add the following two lines.
+ set DOS4GVM=@ALSCRIPT.VMC
+ set DOS4G=quiet
+</OL>
+<P>
+The first line is an instruction to read instructions from the file
+ALSCRIPT.VMC. This sets up a permanent swap file on your hard disk.
+By default, the swap file is about 12MBytes in size. If you do not have
+this much free space on your disk, then edit the ALSCRIPT.VMC file
+to reduce the swap file size, or alternatively, do not put this line
+in your autoexec.bat.
+<P>
+The programs will run without this swap file, but you will be limited in
+ the size of alignment you can process by the amount of RAM you have
+installed. I have only tested this program on a 486/33 with 8MBytes RAM
+and a 386/33 with 4MBytes so I do not know the practical limitations of
+machines with smaller memories. Any feedback would be appreciated.
+<P>
+5. Type AUTOEXEC.BAT to initialise the changes, or better still, reset
+the computer.
+<P>
+6. You should now be able to run all three programs in the package from
+anywhere on your disk. msf2blc, clus2blc and alscript. If you get
+memory allocation errors when you try to run alscript, then use the
+MAX_NSEQ and MAX_SEQ_LEN commands to reduce the default limits. If the
+program still won't run, then think about buying some more memory!!
+<P>
+The programs msf2blc and clus2blc should run OK, but if you try to
+process alignments that are too large for your computer, you may get a
+"malloc error" which will stop the program. If this happens and you are
+not using the virtual memory option discussed above, then try adding the
+line set DOS4GVM=@filename to your autoexec.bat file. If you
+don't have enough disk space to do this, then buy a bigger disk, or more
+memory.
+<P>
+<HR> <A NAME=tex2html426 HREF=subsection3_16_5.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html424 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html418 HREF=subsection3_16_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html428 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html427 HREF=subsection3_16_5.html> TECHNICAL NOTES</A>
+<B>Up:</B> <A NAME=tex2html425 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html419 HREF=subsection3_16_3.html> PostScript Fonts</A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
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+<HEAD>
+<TITLE> TECHNICAL NOTES</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html437 HREF=subsection3_16_6.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html435 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html429 HREF=subsection3_16_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html439 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html438 HREF=subsection3_16_6.html> Unix Installation</A>
+<B>Up:</B> <A NAME=tex2html436 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html430 HREF=subsection3_16_4.html> 386 DOS installation</A>
+<HR> <P>
+<H2><A NAME=SECTION00016500000000000000> TECHNICAL NOTES</A></H2>
+<P>
+The executables included in this package were compiled with the WATCOM C
+compiler. This is a full 32 bit compiler that makes good use of the 386
+processor and does not work on the 16 bit 286. It also has the
+advantage of allowing the flat memory model to be used. In practice
+this means that porting programs like alscript from Unix computers like
+the Sun, is straightforward. In order to access the memory of the
+computer in this way, an extra program called a dos extender is required
+- this is called DOS4GW.EXE. DOS4GW is automatically invoked every time
+you run one of the programs and is responsible for managing the memory and
+creating the swap file discussed above.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE> Unix Installation</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html448 HREF=subsection3_16_7.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html446 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html440 HREF=subsection3_16_5.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html450 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html449 HREF=subsection3_16_7.html> VAX/VMS Installation</A>
+<B>Up:</B> <A NAME=tex2html447 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html441 HREF=subsection3_16_5.html> TECHNICAL NOTES</A>
+<HR> <P>
+<H2><A NAME=SECTION00016600000000000000> Unix Installation</A></H2>
+<P>
+<A NAME=app5><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+ALSCRIPT is distributed with executables for Sun (SunOS 4.1.3), Silicon
+Graphics (IRIX 5.3), DEC ALPHA OSF/1 and Sun Solaris (2.4). The executables
+are stored in the subdirectories bin/sun, bin/sgi, bin/osf and bin/sol. If
+these are OK for your system, then just add the apporpriate directory to your
+path, or put links to /usr/local/bin or somewhere that is on all users paths.
+<P>
+The source code for ALSCRIPT is contained in a directory hierarchy.
+The top directory contains a README file and the BUILD script.
+Subdirectories are: <b>examples</b> which contains example command and
+alignment files, <b>doc</b> which contains <IMG ALIGN=BOTTOM ALT="" SRC="_19723_tex2html_wrap672.xbm"> and PostScript copies
+of the manual - a subdirectory of this contains an HTML version of the
+manual, and <b>src</b> which contains the source code and Makefiles for
+the package. There may also be a directory called <b>bin</b>. If
+present this will contain subdirectories with executables for the
+programs in the package. Makefiles to build alscript, msf2blc,
+clus2blc and alsnum are included in the <b>src</b> directory. Versions
+for Sun (acc compiler .sun), Silicon Graphics (.sgi), DEC OSF/1 (.osf)
+are included.
+<P>
+There is a utility csh script called BUILD. Simply type ./BUILD sun to
+compile alscript on the Sun, ./BUILD sgi for Silicon Graphics or BUILD
+gcc for use with gcc compiler. See instructions in the file BUILD.
+The BUILD script will create a /bin directory and subdirecotry
+if not already present. You can create makefiles for different computers
+and the BUILD script should still function.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> VAX/VMS Installation</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html459 HREF=subsection3_16_8.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html457 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html451 HREF=subsection3_16_6.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html461 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html460 HREF=subsection3_16_8.html> Alternative ways of </A>
+<B>Up:</B> <A NAME=tex2html458 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html452 HREF=subsection3_16_6.html> Unix Installation</A>
+<HR> <P>
+<H2><A NAME=SECTION00016700000000000000> VAX/VMS Installation</A></H2>
+<P>
+<A NAME=app6><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+The standard VAX C compiler is not ANSI. Accordingly, ALSCRIPT will require
+changes to the source code to compile on a VAX.
+<P>
+The DEC C++ compiler works OK for alscript. Alscript will also compile
+on Dec ALPHA under OpenVMS. A descrip.mms file is included for this
+purpose.
+<P>
+<em>WARNING: I've not tested Version 2.0 of ALSCRIPT on VMS</em>
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Alternative ways of invoking ALSCRIPT</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html470 HREF=subsection3_16_9.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html468 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html462 HREF=subsection3_16_7.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html472 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html471 HREF=subsection3_16_9.html> Program Crashes and </A>
+<B>Up:</B> <A NAME=tex2html469 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html463 HREF=subsection3_16_7.html> VAX/VMS Installation</A>
+<HR> <P>
+<H2><A NAME=SECTION00016800000000000000> Alternative ways of invoking ALSCRIPT</A></H2>
+<P>
+<A NAME=app7><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+The documentation above describes the interactive mode of running ALSCRIPT.
+However, it may be more convenient to run the program as a pipe under
+Unix or MS-DOS. Examples are shown here.
+<P>
+ALSCRIPT is a program for producing pretty versions of multiple
+sequence aligments. ALSCRIPT will also format single sequences. A
+full description of the program is given in the file "alscript.doc".
+<P>
+Ways of running alscript:
+<P>
+<OL><LI>
+ Interactive mode: just type alscript.
+ You will be prompted for a command file name. The command file will
+ define the AMPS blocfile, and name of the file to store the PostScript
+ output - see alscript.doc for details.
+<P>
+<LI>
+ alscript <command_file> has same effect as 1, But does not prompt for
+ the command file
+ e.g. alscript example1.als
+<P>
+<LI>
+ alscript -q < <blocfile> > <PostScript>
+ Quick mode - uses default commands, reads alignment from stdin,
+ writes PostScript to stdout. This mode creates a command file
+ called ALPSQ.COM.
+<P>
+ e.g. alscript -q < example1.blc > example1.ps
+<P>
+<LI>
+ alscript -f <command_file>
+ Similar effect to 2.
+<P>
+<LI>
+ alscript -f <command_file> -s
+ Silent operation: No messages are written to stderr, unless fatal.
+ Silent operation may be toggled by the silent_mode command
+ in the command file.
+<P>
+<LI>
+ alscript -f <command_file> -p < <blocfile> > <PostScript>
+ Make alscript work like a pipe - blocfile is read from stdin,
+ postscript is written
+ to stdout. Messages are written to stderr. To supress messages include
+ the -s flag too
+<P>
+ e.g. alscript -f example1.als -p -s < example1.blc > example1.ps
+<P>
+</OL>
+<P>
+Using alscript as a pipe has the advantage of allowing the blocfile to
+be created on the fly by the programs msf2blc or clus2blc. For example
+if we have a GCG .msf file called "pileup.msf" we can run alscript with
+default shading/fonts and send the results straight to the PostScript
+printer "lpr" as follows:
+<P>
+msf2blc -q <pileup.msf | alscript -q -s | lpr
+<P>
+<HR> <A NAME=tex2html470 HREF=subsection3_16_9.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html468 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html462 HREF=subsection3_16_7.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html472 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html471 HREF=subsection3_16_9.html> Program Crashes and </A>
+<B>Up:</B> <A NAME=tex2html469 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html463 HREF=subsection3_16_7.html> VAX/VMS Installation</A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> Program Crashes and Known Bugs</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html481 HREF=subsection3_16_10.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html479 HREF=section3_16.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html473 HREF=subsection3_16_8.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html483 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html482 HREF=subsection3_16_10.html> Wish List for </A>
+<B>Up:</B> <A NAME=tex2html480 HREF=section3_16.html> Appendices</A>
+<B> Previous:</B> <A NAME=tex2html474 HREF=subsection3_16_8.html> Alternative ways of </A>
+<HR> <P>
+<H2><A NAME=SECTION00016900000000000000> Program Crashes and Known Bugs</A></H2>
+<P>
+<A NAME=app8><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/invis_anchor.xbm"></A>
+<P>
+We've used ALSCRIPT on Sun Workstations and Silicon Graphics for some
+time, with very large alignments and command files with thousands of
+commands. All seems to work OK, the program has not crashed on us at
+all!!
+<P>
+However, the command interpreter in ALSCRIPT is very simple and
+the program will crash if you give any command the wrong number of
+arguments (e.g. leaving out the shade value in a shade_chars command).
+<P>
+If you do make the program crash, have checked all the documentation
+and your numbers, and the program still crashes. Then send me
+the command file and block file that causes the crash and I will try
+to investigate.
+<P>
+Suggestions for improvements to the program are always welcome.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> STEP 1 COMMANDS</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html362 HREF=subsubsection3_16_1_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html360 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html354 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html364 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html363 HREF=subsubsection3_16_1_2.html> REQUIRED STEP 1 </A>
+<B>Up:</B> <A NAME=tex2html361 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B> Previous:</B> <A NAME=tex2html355 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<HR> <P>
+<H3><A NAME=SECTION00016110000000000000> STEP 1 COMMANDS</A></H3>
+<P>
+These all refer to either system settings - e.g. the maximum allowed
+sequence length, or to general page layout features. e.g. the longest
+and shortest side of the paper on which you are plotting.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> REQUIRED STEP 1 COMMANDS</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html373 HREF=subsubsection3_16_1_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html371 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html365 HREF=subsubsection3_16_1_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html375 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html374 HREF=subsubsection3_16_1_3.html> OPTIONAL STEP 1 </A>
+<B>Up:</B> <A NAME=tex2html372 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B> Previous:</B> <A NAME=tex2html366 HREF=subsubsection3_16_1_1.html> STEP 1 COMMANDS</A>
+<HR> <P>
+<H3><A NAME=SECTION00016120000000000000> REQUIRED STEP 1 COMMANDS</A></H3>
+<P>
+BLOCK_FILE <string>
+<P>
+Gives the name of the file that contains the multiple sequence alignment
+to be formatted. File names should be fully qualified i.e. not
+relative to the current directory. If no block file command is given,
+ALSCRIPT will expect to read the block file from standard input.
+<P>
+OUTPUT_FILE <string>
+<P>
+Defines the output file name. This command should be near the beginning of
+the command list.
+e.g. OUTPUT_FILE Figure1.ps
+<P>
+You MUST define an output file unless the -p option
+(See <A HREF=subsection3_16_8.html#app7>Alternative ways of invoking ALSCRIPT</A>)
+is used.
+<P>
+DEFINE_FONT <int> <string> (<int>/DEFAULT)/(REL <float>)
+<P>
+Defines a font to use later:
+e.g.
+<PRE><TT>
+DEFINE_FONT 0 Helvetica 10
+DEFINE_FONT 2 Times-Roman 2</TT></PRE>
+<P>
+defines font number 0 to be 10 point Helvetica, and font number 2 to be
+2 point Times-Roman. Font 0 is always used as the default font. You MUST
+define at least the font 0 font.
+<P>
+DEFINE_FONT 3 Times-Roman DEFAULT
+<P>
+sets font 3 to be Times-Roman at whatever the default pointsize
+is as set by the POINTSIZE command.
+<P>
+DEFINE_FONT 4 Helvetica REL 0.5
+<P>
+sets font 4 to be helvetica at half the default pointsize.
+<P>
+NOTE: Font names must be written exactly as shown in
+<A HREF=subsection3_16_3.html#app3>PostScript Fonts</A>.
+<P>
+SETUP
+<P>
+Signals the end of the STEP 1 commands.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> OPTIONAL STEP 1 COMMANDS</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html384 HREF=subsubsection3_16_1_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html382 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html376 HREF=subsubsection3_16_1_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html386 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html385 HREF=subsubsection3_16_1_4.html> STEP 2 COMMANDS</A>
+<B>Up:</B> <A NAME=tex2html383 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B> Previous:</B> <A NAME=tex2html377 HREF=subsubsection3_16_1_2.html> REQUIRED STEP 1 </A>
+<HR> <P>
+<H3><A NAME=SECTION00016130000000000000> OPTIONAL STEP 1 COMMANDS</A></H3>
+<P>
+ADD_SEQ <int> <int>
+<P>
+Allows extra sequence positions to be created in an existing alignment. This
+permits additional annotations to be interspaced either above, below, or
+anywhere in the middle of an alignment. For example:
+<P>
+ADD_SEQ 0 10
+<P>
+would create an additional 10 sequences - all set to the blank character
+before the first sequence in the block file that has been read in.
+<P>
+ADD_SEQ 3 1
+<P>
+would add an extra sequence after sequence 3.
+<P>
+IMPORTANT: If you use the ADD_SEQ facility to add sequences anywhere except
+after the last sequence, then remember that the sequence
+numbers will alter. All formatting commands that follow this command
+must use the new sequence numbering. Thus in the first example:
+<P>
+ADD_SEQ 0 10
+<P>
+what was sequence number 1 becomes sequence 11. Sequences 1-10 are
+the new blank sequences to be used for annotation. Note that the
+sequence numbers only change for commands AFTER the SETUP command,
+thus, multiple add_seq commands refer to the sequence number as
+implied by the block file.
+<P>
+POINTSIZE <int>
+<P>
+Defines the pointsize to be used to scale the plot and space the characters.
+Default is 10 point.
+<P>
+NUMBER_SEQSIf present, then the sequence number is output with the identifier
+code. This is useful for finding the coordinates of residues to box
+or otherwise highlight.
+<P>
+LANDSCAPE
+<P>
+Specifies that alignments will be plotted with the longest paper axis
+horizontal. (Can get longer alignments on a page this way).
+<P>
+PORTRAIT
+<P>
+Specifies that alignments will be plotted with the longest paper axis vertical
+(can get more sequences on a page this way).
+<P>
+IDENT_WIDTH <int>
+<P>
+Units are characters.
+<P>
+Reserves <int> characters at left of every page for plotting
+identifiers. Note that not all this space need be used, if a smaller
+pointsize is used to plot out the identifier codes, than is used for
+the main alignment.
+<P>
+LINE_WIDTH_FACTOR <float>
+<P>
+Value greater than 0 that scales the default line width. The linewidth
+is obtained by multiplying the pointsize by this factor.
+<P>
+X_SPACE_FACTOR <float>
+<P>
+Y_SPACE_FACTOR <float>
+<P>
+This determines the spacing between adjacent residues in the X and Y
+directions. The spacing is calculated as: POINTSIZE + POINTSIZE *
+X_SPACE_FACTORor POINTSIZE + POINTSIZE * Y_SPACE_FACTOR as
+appropriate. Defaults are 0.2 and 0.0 respectively.
+<P>
+X_SHIFT_FACTOR <float>
+<P>
+Y_SHIFT_FACTOR <float>
+<P>
+These determine the shift relative to the residue drawing position
+that is given to the boxing lines. The shift is calculated as
+follows
+<P>
+(POINTSIZE + POINTSIZE * X_SPACE_FACTOR) * X_SHIFT_FACTOR
+similarly for Y_SHIFT_FACTOR.
+<P>
+The defaults are 0.3 and 0.0 respectively.
+<P>
+Fiddling with the X_SPACE/SHIFT values is useful to fine tune the
+appearance of the alignment.
+<P>
+MAX_INPUT_LEN <int>
+<P>
+Units are characters.
+Defines the maximum number of characters possible in the input line
+length. This must be greater than the maximum number of sequences
+(MAX_NSEQ).
+<P>
+e.g. MAX_INPUT_LEN 600
+<P>
+Increases the default value of 500 characters to 600 characters.
+<P>
+MAX_NSEQ <int>
+<P>
+Units are characters. Defines the maximum number of sequences that
+may be read by the program. This parameter has a large default (500).
+You may need to reduce it on computers with small memories.
+<P>
+MAX_ILEN <int>
+<P>
+Units are characters.
+The maximum length allowed for a sequence identifier code.
+<P>
+MAX_SEQ_LEN <int>
+<P>
+Defines the maximum length allowed for a sequence alignment - this may
+need to be reduced from the 8000 default value on smaller computers.
+<P>
+X_OFFSET <int>
+Units of points (1/72 inch).
+<P>
+Defines the offset along the X-axis that the alignments will be shifted prior
+to printing. Fiddle with this value to get a nice offset from the bottom left
+hand corner of the page if your page size is not A4.
+<P>
+Y_OFFSET <int>
+Units of points (1/72 inch).
+<P>
+As for X_OFFSET, only Y axis.
+<P>
+MAX_SIDE <int>
+Units of inches.
+<P>
+Defines the length of the longest side of the printer page.
+<P>
+MIN_SIDE <int>
+Units of inches.
+<P>
+Defines the length of the shortest side of the printer page.
+<P>
+VERTICAL_SPACING <int>
+<P>
+Defines the vertical spacing in character units between blocks of sequences
+when more than one block will fit on a page - default is 0.
+<P>
+DEFINE_COLOUR <int> <float> <float> <float>
+<P>
+DEFINE_COLORDefines a colour - the first number is a number by which the colour will
+be referred. The following three numbers are the intensities of red,
+green and blue respectively. Thus:
+<P>
+DEFINE_COLOUR 1 0 0.2 0.8
+<P>
+sets colour number 1 to be a colour with no red, 0.2 green and 0.8 blue.
+The exact appearance of this colour will depend on the output device.
+If you find suitable combinations of colours for your printer, then
+please let me know and I shall distribute your suggestions with the
+program.
+<P>
+DO_TICKSIf present, then tick marks are drawn below the numbers at the top of the page.
+Otherwise no ticks are shown.
+<P>
+NUMBER_INT <int>
+<P>
+Specifies the interval for writing residue position numbers. Default is 10
+<P>
+NO_NUMBERSSwitches all residue numbering off.
+<P>
+<HR> <A NAME=tex2html384 HREF=subsubsection3_16_1_4.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html382 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html376 HREF=subsubsection3_16_1_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html386 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html385 HREF=subsubsection3_16_1_4.html> STEP 2 COMMANDS</A>
+<B>Up:</B> <A NAME=tex2html383 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B> Previous:</B> <A NAME=tex2html377 HREF=subsubsection3_16_1_2.html> REQUIRED STEP 1 </A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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+<HEAD>
+<TITLE> STEP 2 COMMANDS</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html393 HREF=subsection3_16_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html391 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html387 HREF=subsubsection3_16_1_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html395 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html394 HREF=subsection3_16_2.html> AMPS Block file </A>
+<B>Up:</B> <A NAME=tex2html392 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B> Previous:</B> <A NAME=tex2html388 HREF=subsubsection3_16_1_3.html> OPTIONAL STEP 1 </A>
+<HR> <P>
+<H3><A NAME=SECTION00016140000000000000> STEP 2 COMMANDS</A></H3>
+<P>
+All these are optional formatting commands.
+<P>
+IMPORTANT PLEASE READ THIS NOTE:
+<P>
+For those commands that accept region definitions (e.g. SURROUND_CHARS) it
+is easiest to think of the region being defined in terms of X and Y
+coordinates, where X is the sequence residue coordinate and Y is the
+sequence number coordinate. Thus 3 7 means the 3rd residue in sequence 7.
+3 7 12 42 means the rectangular box bounded by residue 3 of sequence 7 and
+residue 12 of sequence 42.
+<P>
+SURROUND_CHARS <string> ALL
+<P>
+Draw lines round, but not between the characters that are in the string.
+e.g.
+<P>
+SURROUND_CHARS GP ALL
+<P>
+will draw lines round all G and P characters in the alignment, but not
+between adjacent G and P characters.
+<P>
+SURROUND_CHARS <string> <int> <int> <int> <int>
+<P>
+Similar command, but the surrounding is restricted to the region defined by
+the four integers.
+<P>
+e.g.
+<P>
+SURROUND_CHARS ILVW 3 12 7 32
+<P>
+would surround ILVW characters that occur in the region defined
+from residue positions 3-7 of sequences 12 to 32.
+<P>
+SHADE_CHARS <string> ALL <float>
+<P>
+Shade all characters in the <string> by the grey value given by <float>.
+e.g.
+<P>
+SHADE_CHARS GP ALL 0.5
+<P>
+would shade all G and P characters in the alignment by the grey value 0.5.
+<P>
+SHADE_CHARS <string> <int> <int> <int> <int> <float>
+<P>
+restricts the shading to the region defined by the four integers. Thus
+<P>
+SHADE_CHARS ILVW 3 12 7 32 0.7
+<P>
+would shade I L V and W characters from residues 3-7 of sequences 12-32
+inclusive with a grey value of 0.7.
+<P>
+FONT_CHARS <string> ALL <int>
+<P>
+e.g.
+<P>
+FONT_CHARS GP ALL 7
+<P>
+would use font 7 to write out all G and P characters. Font 7 MUST have been
+defined using the DEFINE_FONTcommands above.
+<P>
+FONT_CHARS <string> <int> <int> <int> <int> <int>
+<P>
+Similar to previous command, but restricts the effect to the region defined
+by the first four integers. The font must have been defined by the
+DEFINE_FONT command.
+<P>
+e.g.
+<P>
+FONT_CHARS ILVW 3 45 9 70 7
+<P>
+Would set the font to 7 for I L V and W characters for residues 3-9 of
+sequences 45-70 inclusive. The font must have been defined by the
+DEFINE_FONT command.
+<P>
+FONT_REGION <int> <int> <int> <int> <int>
+<P>
+Define the font to use throughout the region specified by the first four
+integers.
+<P>
+e.g.
+<P>
+FONT_REGION 3 12 20 40 10
+<P>
+Use font 10 for residues from residues 3-20 of sequences 12-40. The font
+must have been defined using the DEFINE_FONTcommand.
+<P>
+FONT_RESIDUE <int> <int> <int>
+<P>
+Set the font for use with a single residue position - most useful when used
+with the TEXT command.
+<P>
+e.g.
+<P>
+FONT_RESIDUE 3 7 2
+<P>
+Use font 2 for residue 3 of sequence 7. Font 2 must have been defined using
+the DEFINE_FONT command.
+<P>
+LINE <string> <int> <int> <int>
+<P>
+There are four commands of this type for drawing horizontal or vertical lines
+on the alignment.
+<P>
+LINE LEFT <int> <int> <int>
+<P>
+Draw a line to the left of the character positions indicated.
+<P>
+e.g.
+<P>
+LINE LEFT 3 12 24
+<P>
+Draw a vertical line starting at residue 3 of sequence 12 and ending at
+residue 3 of sequence 24.
+<P>
+LINE TOP 3 12 24
+<P>
+Draw a horizontal line above the character positions from residue 3 of
+sequence 12 to residue 24 of sequence 12.
+<P>
+Similar commands are:
+<P>
+LINE BOTTOM <int> <int> <int> Draw a line at bottom of character position.
+<P>
+LINE RIGHT <int> <int> <int> Draw a line at right of character position.
+<P>
+BOX_REGION <int> <int> <int> <int>
+<P>
+Draw a box around the region indicated by the four integers.
+<P>
+e.g.
+<P>
+BOX_REGION 2 5 30 7
+<P>
+Would box from residue 2 of sequence 5 to residue 30 of sequence 7.
+<P>
+SHADE_REGION <int> <int> <int> <int> <float>
+<P>
+Shade the region indicated by the integers with the grey value shown by the
+float.
+e.g.
+<P>
+SHADE_REGION 30 40 35 46 0.2
+<P>
+Would shade from residue 30-35 of sequences 40-46 with a grey value of 0.2.
+<P>
+SHADE_RES <int> <int> <float>
+<P>
+Shade just one amino acid with the grey value.
+<P>
+e.g.
+<P>
+SHADE_RES 3 4 0.7
+<P>
+Shades residue 3 of sequence 7. (Note: this can also be achieved with the
+<P>
+SHADE_REGION command, but requires 2 extra numbers)
+<P>
+TEXT <int> <int> <qstring>
+<P>
+Place the text string at the location indicated.
+<P>
+e.g.
+<P>
+TEXT 30 70 "Active Site His"
+<P>
+would put the text Active Site His starting at position 30 of sequence
+70. (Use FONT_RESIDUE or FONT_REGION commands to set the font of the
+text). Text added with the TEXT command will not be split across page
+breaks, so you may in some circumstances need to fiddle a little with
+the location/pointsize for the text to get the desired result.
+<P>
+ID_FONT ALL <int>
+<P>
+Set the font for all identifier codes to the font number shown by <int>.
+e.g.
+<P>
+ID_FONT ALL 3
+<P>
+Would set all the identifier codes to font 3.
+<P>
+ID_FONT <int> <int>
+<P>
+Set the font for a specific identifier to font number.
+e.g.
+<P>
+ID_FONT 12 4
+<P>
+Use font 4 for the identifer of sequence 12, default font for all other
+identifiers.
+<P>
+SUB_CHARS ALL <char> <char>
+<P>
+Substitute the characters indicated.
+<P>
+e.g.
+<P>
+SUB_CHARS ALL + *
+<P>
+would change all occurences of + to * in the alignment.
+<P>
+SUB_CHARS <int> <int> <int> <int> <char> <char>
+<P>
+restrict the substitution to the region shown.
+<P>
+e.g.
+<P>
+SUB_CHARS 1 1 7 8 %*
+<P>
+would substitute * for %from residue 1-7 of sequences 1-8.
+NOTE: To substitute for or with the space character use the word SPACE.
+e.g. to change all space characters to -.
+<P>
+SUB_CHARS ALL SPACE -
+<P>
+SUB_ID <int> <qstring>
+<P>
+Replace the numbered identifier by the string.
+e.g.
+<P>
+SUB_ID 34 "Predicted Secondary Structure"
+<P>
+would replace whatever the identifier of sequence 34 was, by the text shown.
+This is useful when used in conjunction with the ADD_SEQ command shown under
+the STEP 1 commands.
+<P>
+INVERSE_CHARS <string> ALL/Range (similar syntax to FONT_CHARS but no
+font number)
+<P>
+Print the selected characters in white. This clearly will only work
+if you first use the SHADE_CHARScommand to shade the characters with
+something other than white.
+<P>
+CCOL_CHARS <string> ALL <int>
+<P>
+Colour all characters in the <string> by the colour defined by <int>.
+<P>
+e.g.
+<P>
+CCOL_CHARS GP ALL 12
+<P>
+would colour all G and P characters in the alignment by the colour 12.
+This colour MUST have been defined by the DEFINE_COLOUR command.
+<P>
+CCOL_CHARS <string> <int> <int> <int> <int> <int>
+<P>
+restricts the colouring to the region defined by the four integers. Thus
+<P>
+CCOL_CHARS ILVW 3 12 7 32 7
+<P>
+would colour I L V and W characters from residues 3-7 of sequences 12-32
+inclusive with the colour 7.
+<P>
+SCOL_CHARS: This has identical syntax to SCOL_CHARS, but colours the
+background of the character, rather than the letter itself.
+<P>
+COLOUR_REGION <int> <int> <int> <int> <int>
+<P>
+COLOR_REGIONColour the region indicated by the integers with the colour number given
+as the last number.
+<P>
+e.g.
+<P>
+COLOUR_REGION 30 40 35 46 2
+<P>
+Would colour from residue 30-35 of sequences 40-46 with the colour 2.
+<P>
+COLOUR_RES <int> <int> <int>
+<P>
+Colour just one amino acid with the defined colour.
+<P>
+e.g.
+<P>
+COLOUR_RES 3 4 7
+<P>
+Colours residue 3 of sequence 7. (Note: this can also be achieved with the
+COLOUR_REGION command, but requires 2 extra numbers)
+<P>
+<HR> <A NAME=tex2html393 HREF=subsection3_16_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html391 HREF=subsection3_16_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html387 HREF=subsubsection3_16_1_3.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <A NAME=tex2html395 HREF=tableofcontents3_1.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/contents_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html394 HREF=subsection3_16_2.html> AMPS Block file </A>
+<B>Up:</B> <A NAME=tex2html392 HREF=subsection3_16_1.html> ALSCRIPT Command Summary</A>
+<B> Previous:</B> <A NAME=tex2html388 HREF=subsubsection3_16_1_3.html> OPTIONAL STEP 1 </A>
+<HR> <P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+<HEAD>
+<TITLE>Contents</TITLE>
+</HEAD>
+<BODY><P>
+ <HR> <A NAME=tex2html51 HREF=section3_2.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/next_motif.gif"></A> <A NAME=tex2html49 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/up_motif.gif"></A> <A NAME=tex2html43 HREF=alscript.html><IMG ALIGN=MIDDLE SRC="http://geoff.biop.ox.ac.uk/latex2html/previous_motif.gif"></A> <BR>
+<B> Next:</B> <A NAME=tex2html52 HREF=section3_2.html> Update History</A>
+<B>Up:</B> <A NAME=tex2html50 HREF=alscript.html>No Title</A>
+<B> Previous:</B> <A NAME=tex2html44 HREF=alscript.html>No Title</A>
+<HR> <P>
+<P><H2><A NAME=SECTION0001000000000000000>Contents</A></H2>
+<UL>
+<LI> <A NAME=tex2html53 HREF=tableofcontents3_1.html#SECTION0001000000000000000>Contents</A>
+<LI> <A NAME=tex2html54 HREF=section3_2.html#SECTION0002000000000000000> Update History</A>
+<LI> <A NAME=tex2html55 HREF=section3_3.html#SECTION0003000000000000000> Read This First - VERSION 2.0</A>
+<LI> <A NAME=tex2html56 HREF=section3_4.html#SECTION0004000000000000000> Related Programs</A>
+<LI> <A NAME=tex2html57 HREF=section3_5.html#SECTION0005000000000000000> Availability</A>
+<LI> <A NAME=tex2html58 HREF=section3_6.html#SECTION0006000000000000000> Installing ALSCRIPT</A>
+<LI> <A NAME=tex2html59 HREF=section3_7.html#SECTION0007000000000000000> Brief Description of ALSCRIPT</A>
+<LI> <A NAME=tex2html60 HREF=section3_8.html#SECTION0008000000000000000> New Features in Version 1.4.4</A>
+<LI> <A NAME=tex2html61 HREF=section3_9.html#SECTION0009000000000000000> New Features in Version 1.4.5 - Program alsnum</A>
+<LI> <A NAME=tex2html62 HREF=section3_10.html#SECTION00010000000000000000> New Features in Version 2.0</A>
+<UL>
+<LI> <A NAME=tex2html63 HREF=subsection3_10_1.html#SECTION00010100000000000000> New Step 1 Commands</A>
+<LI> <A NAME=tex2html64 HREF=subsection3_10_2.html#SECTION00010200000000000000> New Step 2 Commands</A>
+<LI> <A NAME=tex2html65 HREF=subsection3_10_3.html#SECTION00010300000000000000> New special TEXT commands</A>
+</UL>
+<LI> <A NAME=tex2html66 HREF=section3_11.html#SECTION00011000000000000000> Running ALSCRIPT</A>
+<UL>
+<LI> <A NAME=tex2html67 HREF=subsection3_11_1.html#SECTION00011100000000000000> Basic Use</A>
+<LI> <A NAME=tex2html68 HREF=subsection3_11_2.html#SECTION00011200000000000000> More complex effects - Text Lines, and Masks</A>
+</UL>
+<LI> <A NAME=tex2html69 HREF=section3_12.html#SECTION00012000000000000000> Using Colour</A>
+<LI> <A NAME=tex2html70 HREF=section3_13.html#SECTION00013000000000000000> The MASK command family</A>
+<UL>
+<LI> <A NAME=tex2html71 HREF=subsection3_13_1.html#SECTION00013100000000000000> Summary of mask commands</A>
+</UL>
+<LI> <A NAME=tex2html72 HREF=section3_14.html#SECTION00014000000000000000> Printing ALSCRIPT Files</A>
+<LI> <A NAME=tex2html73 HREF=section3_15.html#SECTION00015000000000000000> Conclusion</A>
+<LI> <A NAME=tex2html74 HREF=section3_16.html#SECTION00016000000000000000> Appendices</A>
+<UL>
+<LI> <A NAME=tex2html75 HREF=subsection3_16_1.html#SECTION00016100000000000000> ALSCRIPT Command Summary</A>
+<UL>
+<LI> <A NAME=tex2html76 HREF=subsubsection3_16_1_1.html#SECTION00016110000000000000> STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html77 HREF=subsubsection3_16_1_2.html#SECTION00016120000000000000> REQUIRED STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html78 HREF=subsubsection3_16_1_3.html#SECTION00016130000000000000> OPTIONAL STEP 1 COMMANDS</A>
+<LI> <A NAME=tex2html79 HREF=subsubsection3_16_1_4.html#SECTION00016140000000000000> STEP 2 COMMANDS</A>
+</UL>
+<LI> <A NAME=tex2html80 HREF=subsection3_16_2.html#SECTION00016200000000000000> AMPS Block file format</A>
+<LI> <A NAME=tex2html81 HREF=subsection3_16_3.html#SECTION00016300000000000000> PostScript Fonts</A>
+<LI> <A NAME=tex2html82 HREF=subsection3_16_4.html#SECTION00016400000000000000> 386 DOS installation</A>
+<LI> <A NAME=tex2html83 HREF=subsection3_16_5.html#SECTION00016500000000000000> TECHNICAL NOTES</A>
+<LI> <A NAME=tex2html84 HREF=subsection3_16_6.html#SECTION00016600000000000000> Unix Installation</A>
+<LI> <A NAME=tex2html85 HREF=subsection3_16_7.html#SECTION00016700000000000000> VAX/VMS Installation</A>
+<LI> <A NAME=tex2html86 HREF=subsection3_16_8.html#SECTION00016800000000000000> Alternative ways of invoking ALSCRIPT</A>
+<LI> <A NAME=tex2html87 HREF=subsection3_16_9.html#SECTION00016900000000000000> Program Crashes and Known Bugs</A>
+<LI> <A NAME=tex2html88 HREF=subsection3_16_10.html#SECTION000161000000000000000> Wish List for next version!!</A>
+<LI> <A NAME=tex2html89 HREF=subsection3_16_11.html#SECTION000161100000000000000> Acknowledgements</A>
+<LI> <A NAME=tex2html90 HREF=subsection3_16_12.html#SECTION000161200000000000000> References</A>
+</UL>
+<LI> <A NAME=tex2html91 HREF=section3_17.html#SECTION00017000000000000000> <em>About this document ...</em> </A>
+</UL>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
\ No newline at end of file
--- /dev/null
+#Comments in ALscript command files start with a #
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 24 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+SETUP #Tell the program to get on with the formatting.
+
--- /dev/null
+A simple example block file. The important parts are the lines that
+start with the greater than character - these are identifer lines for the
+sequences. The first star is above the position of the first sequence
+in the alingmnet which is written VERTICALLY down the page. The second
+star shows the end of the alignment.
+
+Anything else can be written here, except greater than signs and stars.
+
+This is the format produced by the AMPS package for multiple
+sequence alignment.
+
+
+>one
+>two
+>three
+>four
+>five
+*
+ABCDE
+EDFTD
+AAAAA
+LLL
+PPPPP
+GGGGG
+SSSSS
+KLMNT
+TTLPQ
+SLNST
+TTLSN
+LQPPL
+LLLLL
+TTTTT
+*
--- /dev/null
+%!PS-Adobe-2.0
+%%Creator: ALSCRIPT Version 2.04 - 28 June 1996
+%%Title: Alscript Output
+% Barton, G. J. (1993)
+% ALSCRIPT - A Tool to format multiple sequence alignments
+% Protein Engineering, 6, 37-40
+%
+/P {moveto show} def
+/L {moveto lineto stroke} def
+/ML {moveto lineto lineto lineto lineto} def
+2 setlinecap
+/C99 {1.000000 1.000000 1.000000 setrgbcolor } def
+/C100 {0.000000 0.000000 0.000000 setrgbcolor } def
+120 currentscreen 3 -1 roll pop setscreen
+/F0 {/Helvetica findfont 24.00 scalefont setfont } def
+%%Page: ? 1
+508 30 translate
+90 rotate
+F0
+ 0 288 translate
+2 setlinecap
+ 1.20 setlinewidth
+ 0 0 moveto (five
+) show
+ 0 24 moveto (four
+) show
+ 0 48 moveto (three
+) show
+ 0 72 moveto (two
+) show
+ 0 96 moveto (one
+) show
+(E) 280 0 P
+(D) 308 0 P
+(A) 336 0 P
+(P) 392 0 P
+(G) 420 0 P
+(S) 448 0 P
+(T) 476 0 P
+(Q) 504 0 P
+(T) 532 0 P
+(N) 560 0 P
+(L) 588 0 P
+(L) 616 0 P
+(T) 644 0 P
+(D) 280 24 P
+(T) 308 24 P
+(A) 336 24 P
+(P) 392 24 P
+(G) 420 24 P
+(S) 448 24 P
+(N) 476 24 P
+(P) 504 24 P
+(S) 532 24 P
+(S) 560 24 P
+(P) 588 24 P
+(L) 616 24 P
+(T) 644 24 P
+(C) 280 48 P
+(F) 308 48 P
+(A) 336 48 P
+(L) 364 48 P
+(P) 392 48 P
+(G) 420 48 P
+(S) 448 48 P
+(M) 476 48 P
+(L) 504 48 P
+(N) 532 48 P
+(L) 560 48 P
+(P) 588 48 P
+(L) 616 48 P
+(T) 644 48 P
+(B) 280 72 P
+(D) 308 72 P
+(A) 336 72 P
+(L) 364 72 P
+(P) 392 72 P
+(G) 420 72 P
+(S) 448 72 P
+(L) 476 72 P
+(T) 504 72 P
+(L) 532 72 P
+(T) 560 72 P
+(Q) 588 72 P
+(L) 616 72 P
+(T) 644 72 P
+(A) 280 96 P
+(E) 308 96 P
+(A) 336 96 P
+(L) 364 96 P
+(P) 392 96 P
+(G) 420 96 P
+(S) 448 96 P
+(K) 476 96 P
+(T) 504 96 P
+(S) 532 96 P
+(T) 560 96 P
+(L) 588 96 P
+(L) 616 96 P
+(T) 644 96 P
+ 280 120 moveto (1) show
+ 532 120 moveto (10) show
+showpage
--- /dev/null
+#SILENT_MODE
+# Illustration of ALSCRIPT Command File
+#
+# As shown in: Barton, G. J. (1992)
+# ALSCRIPT- A tool to format multiple sequence alignments
+# Protein Engineering (in press)
+#
+# example 1 # Comment lines begin with the # symbol
+#
+#
+# (A) Input and output files:
+#
+BLOCK_FILE example1.blc # Define the block file to format
+OUTPUT_FILE example1.ps # Where to put the result
+#
+MAX_SIDE 11 # Fiddle with margins...
+Y_OFFSET 50 # This is just to fill the page with the
+X_OFFSET 80 # Alignment. Defaults normally work OK
+#
+# (B) Page Layout and overall spacing:
+#
+ADD_SEQ 38 1 # Make space after sequence 38
+ADD_SEQ 39 3 # Make space after sequence 39
+ADD_SEQ 69 1
+PORTRAIT # Portrait paper orientation
+POINTSIZE 10 # 10 point default pointsize
+#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot
+IDENT_WIDTH 8
+#
+# (C) Font definitions:
+#
+DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize
+DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT
+DEFINE_FONT 4 Times-Bold DEFAULT
+DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT
+DEFINE_FONT 6 Times-Roman DEFAULT
+SETUP # Tell the program to get on with the formatting
+#
+# (D) Some formatting commands:
+#
+SURROUND_CHARS LIV ALL # Surround all L and Y characters
+FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters
+FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps
+FONT_CHARS KR ALL 4 # Show K and R in Times-Bold
+BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16
+SHADE_CHARS + ALL 0.0 # Shade all + characters black
+SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40
+LINE TOP 2 40 8 # Draw horizontal lines to
+LINE BOTTOM 2 40 8 # Join up surrounded numbers
+TEXT 2 42 "Helix Pattern"
+TEXT 12 42 "Glycine Loop" # Add text annotations
+TEXT 21 42 "Helix Pattern"
+SUB_ID 49 "Helix Predictions"
+SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms
+SUB_ID 68 "Turn Predictions"
+INVERSE_CHARS DE ALL
+SHADE_CHARS D ALL 0.5
+SHADE_CHARS E ALL 0.0
+#
+# (E) Now make the prediction histograms nicer.
+#
+SURROUND_CHARS H 1 44 27 53
+SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey
+SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H"
+#
+SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions
+SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions
+SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions
+#
+SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions
+SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions
+SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions
+#
+SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction
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+SHADE_CHARS T 1 75 27 75 0.9
+#
+#
+# Now change the identifier codes
+#
+ID_FONT ALL 6
+SUB_ID 1 "Annexin I H4"
+SUB_ID 2 "Annexin I M4"
+SUB_ID 3 "Annexin I R4"
+SUB_ID 4 "Annexin V R4"
+SUB_ID 5 "Annexin V H4"
+SUB_ID 6 "Annexin V C4"
+SUB_ID 7 "Annexin II H4"
+SUB_ID 8 "Annexin II B4"
+SUB_ID 9 "Annexin II M4"
+SUB_ID 10 "Annexin IV P4"
+SUB_ID 11 "Annexin IV B4"
+SUB_ID 12 "Annexin IV H4"
+SUB_ID 13 "Annexin VI H4"
+SUB_ID 14 "Annexin VI M4"
+SUB_ID 15 "Annexin VI H8"
+SUB_ID 16 "Annexin VI M8"
+SUB_ID 17 "Annexin V BH4"
+SUB_ID 18 "Annexin VII 4"
+SUB_ID 19 "Annexin III H4"
+SUB_ID 20 "Annexin III R4"
+SUB_ID 21 "Annexin II M3"
+SUB_ID 22 "Annexin II H3"
+SUB_ID 23 "Annexin II B3"
+SUB_ID 24 "Annexin I H3"
+SUB_ID 25 "Annexin I M3"
+SUB_ID 26 "Annexin I R3"
+SUB_ID 27 "Annexin V C3"
+SUB_ID 28 "Annexin V H3"
+SUB_ID 29 "Annexin V R3"
+SUB_ID 30 "Annexin VI H3"
+SUB_ID 31 "Annexin VI M3"
+SUB_ID 32 "Annexin IV H3"
+SUB_ID 33 "Annexin IV B3"
+SUB_ID 34 "Annexin IV P3"
+SUB_ID 35 "Annexin V BH3"
+SUB_ID 36 "Annexin III H3"
+SUB_ID 37 "Annexin III R3"
+SUB_ID 38 "Annexin VII 3"
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+>L1H4
+>L1M4
+>L1R4
+>L5R4
+>L5H4
+>L5C4
+>L2H4
+>L2B4
+>L2M4
+>L4P4
+>L4B4
+>L4H4
+>L6H4
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+>L6H8
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+>L5BH4
+>p474
+>L3H4
+>L3R4
+>L2M3
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+>L2B3
+>L1H3
+>L1M3
+>L1R3
+>L5C3
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+>L6M3
+>L4H3
+>L4B3
+>L4P3
+>L5BH3
+>L3H3
+>L3R3
+>p473
+>Conservation
+>
+>
+>
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+>
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+>
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+>
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+>
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+>
+>
+>
+ *
+ FFFLLFFFFFFFFFFFFFLLDDDDDDDDDDDDDDDDDD HHHHHHH E TTTTH
+ AAAAAAAAAAAAAAAAAAAAAAAAAAAAAVVAAAAAAA7 HHHHHHHH TTTH
+ EEEEEEDDDEEEEEDDEEEGRRRRRRQQQQQQQQQQQQ HHHHHHHH TTH
+ KKKTTTRRRRRKRRKKRRRREDDAAAVAADDDDDDITR HHHHHHHHH E TTTH
+ LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLL+ HHHHHHHHH E TTTH
+ HYYYYYYYYYYYFFYYYYHHYYYYYYFFFYYYYYYYYY HHHHHHHHH E TTH
+ QEEYYYDDDKKKKKKKYYRQDDDEEERQQEEEEEAKDQ HHHHHHHHH E TTH
+ AAAAASSSSSSSAASSAAAAAAAAAAAAAAAAAAAAAA7 HHHHHHHH E TTH
+ GGGGGGGGGGGGGGGGGG H T
+ MMMMMMMMMMMMMMMMMMLLVVVEEEEEEEEEEEEEEE HHHHHHHH E TTTH
+ KKKKKKKKKKKKKKKKKKKKKKKRRRLLLLLKKKKNKG HHHHHHH E TTTTH
+ GGGGGGGGGGGGGGGGGGGGRRRRRRKKKKKKKKIRKR HHH TTTTT
+ VAAAAAKKKLLLLLAAAAIAKKKKKKWWWWWWWWRWWL HHH TTTTT
+ GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG+ HH TTTTTTT
+ TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5 HHHH TTTTTTTTTT
+ RRRDDDRRRDDDRRDDRDDDDDDDDDDDDDDDDDDDDD HHHHH TTTTTTTTTT
+ HHHDDDDDDDDDDDEEDDEEVVVVVVEEEEEEEEEEEE HHHHHHHH E TTTTTTTTTT
+ KKKHHDKKKNDNNNKKGSFFPPPNNNEEEAAVVVMDDS HHHHHHH E TTTTTTTY
+ AATTTTVVVTTTTTTTTTTTKKKVVVTKKQQKKKKKKC HHHHHHHH EEE TTTTTH
+ LLLLLLLLLLLLLLLLLLLLWWWFFFFFFFFFFFFFFF6 HHHHHHHH EEEE TTH
+ IIIIIIIIIIIIIITTIVNNIIINTNIIIIILLLITTN5 HHHHHHH EEEE TH
+ RRRRRRRRRRRRRRRRRRRRSSSTTTTTTYYTTTTEEM HHHHHHH EEEEE H
+ IIIVVVIIIVVVIIIVNIIIIIIIIIIIIIIVVVIIII5 HHHHHH EEEEE H
+ MMMIMMMMMMMMMMMMIVMMMMMLLLLFLLLLLLLLLL6 HHHHHH EEEEE TH
+ VVVVVVVVVVVVVVVVVVVVTTTTTTGGGGGCCCCCCA6 HHHHH EEEE TTTTH
+ SSSSSSSSSSSSSSSSSTSSEEETSTTTTNNSSSTLLT HHHH EE TTTTTT
+ RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR+ HHHH EE TTTTTTTTTT
+ *
+
+
+
--- /dev/null
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+ 96 750 moveto (1) show
+ 204 750 moveto (10) show
+ 324 750 moveto (20) show
+showpage
--- /dev/null
+# FILE example2.com
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example2.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica
+DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic
+NUMBER_SEQS #Number the sequences at left hand side
+SETUP #Tell the program to get on with the formatting.
+#
+#step 2 commands come after the SETUP command
+#
+#Here are some examples...
+#
+SURROUND_CHARS GP ALL #draw lines around all G and P
+SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6
+BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20
+FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters
+ID_FONT ALL 1 #set identifiers in font 1
--- /dev/null
+# Illustration of ALSCRIPT Command File
+#
+# As shown in: Barton, G. J. (1992)
+# ALSCRIPT- A tool to format multiple sequence alignments
+# Protein Engineering (in press)
+#
+# Modified version - includes colour commands
+#
+#
+# example 1 # Comment lines begin with the # symbol
+#
+#
+# (A) Input and output files:
+#
+BLOCK_FILE example1.blc # Define the block file to format
+OUTPUT_FILE example3.ps # Where to put the result
+#
+MAX_SIDE 11 # Fiddle with margins...
+Y_OFFSET 50 # This is just to fill the page with the
+X_OFFSET 80 # Alignment. Defaults normally work OK
+#
+# (B) Page Layout and overall spacing:
+#
+ADD_SEQ 38 1 # Make space after sequence 38
+ADD_SEQ 39 3 # Make space after sequence 39
+ADD_SEQ 69 1
+PORTRAIT # Portrait paper orientation
+POINTSIZE 10 # 10 point default pointsize
+#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot
+IDENT_WIDTH 8
+#
+# (C) Font definitions:
+#
+DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize
+DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT
+DEFINE_FONT 4 Times-Bold DEFAULT
+DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT
+DEFINE_FONT 6 Times-Roman DEFAULT
+#
+# COLOUR DEFINITIONS give a colour number, then red, green, blue intensities
+#
+# red
+DEFINE_COLOUR 0 1 0 0
+# green
+DEFINE_COLOUR 1 0 1 0
+# blue
+DEFINE_COLOUR 2 0 0 1
+# yellow
+DEFINE_COLOUR 3 1 1 0
+# magenta
+DEFINE_COLOUR 4 1 0 1
+# cyan
+DEFINE_COLOUR 5 0 1 1
+#
+DEFINE_COLOUR 6 0.7 0.3 0
+#single_page
+#background_colour 100
+SETUP # Tell the program to get on with the formatting
+#background_region -10 -10 468 792
+#
+# (D) Some formatting commands:
+#
+SURROUND_CHARS LIV ALL # Surround all L and Y characters
+FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters
+FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps
+FONT_CHARS KR ALL 4 # Show K and R in Times-Bold
+Ccol_CHARS LIV ALL 1
+Scol_CHARS ASN ALL 0
+BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16
+SHADE_CHARS + ALL 0.0 # Shade all + characters black
+COLOUR_REGION 14 1 16 38 2
+SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40
+LINE TOP 2 40 8 # Draw horizontal lines to
+LINE BOTTOM 2 40 8 # Join up surrounded numbers
+TEXT 2 42 "Helix Pattern"
+TEXT 12 42 "Glycine Loop" # Add text annotations
+TEXT 21 42 "Helix Pattern"
+SUB_ID 49 "Helix Predictions"
+SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms
+SUB_ID 68 "Turn Predictions"
+INVERSE_CHARS DE ALL
+SHADE_CHARS D ALL 0.5
+SHADE_CHARS E ALL 0.0
+#
+# (E) Now make the prediction histograms nicer.
+#
+SURROUND_CHARS H 1 44 27 53
+Scol_CHARS H 1 44 27 53 3 # Shade the Helix predictions 0.5 grey
+SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H"
+#
+SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions
+Scol_CHARS E 1 54 27 63 4 # Colour beta blue
+SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions
+#
+SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions
+Scol_CHARS T 1 64 27 73 6 # Shade the Turn Predictions
+SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions
+#
+SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction
+SHADE_CHARS HY 1 75 27 75 0.5
+SHADE_CHARS T 1 75 27 75 0.9
+#
+#
+# Now change the identifier codes
+#
+ID_FONT ALL 6
+SUB_ID 1 "Annexin I H4"
+SUB_ID 2 "Annexin I M4"
+SUB_ID 3 "Annexin I R4"
+SUB_ID 4 "Annexin V R4"
+SUB_ID 5 "Annexin V H4"
+SUB_ID 6 "Annexin V C4"
+SUB_ID 7 "Annexin II H4"
+SUB_ID 8 "Annexin II B4"
+SUB_ID 9 "Annexin II M4"
+SUB_ID 10 "Annexin IV P4"
+SUB_ID 11 "Annexin IV B4"
+SUB_ID 12 "Annexin IV H4"
+SUB_ID 13 "Annexin VI H4"
+SUB_ID 14 "Annexin VI M4"
+SUB_ID 15 "Annexin VI H8"
+SUB_ID 16 "Annexin VI M8"
+SUB_ID 17 "Annexin V BH4"
+SUB_ID 18 "Annexin VII 4"
+SUB_ID 19 "Annexin III H4"
+SUB_ID 20 "Annexin III R4"
+SUB_ID 21 "Annexin II M3"
+SUB_ID 22 "Annexin II H3"
+SUB_ID 23 "Annexin II B3"
+SUB_ID 24 "Annexin I H3"
+SUB_ID 25 "Annexin I M3"
+SUB_ID 26 "Annexin I R3"
+SUB_ID 27 "Annexin V C3"
+SUB_ID 28 "Annexin V H3"
+SUB_ID 29 "Annexin V R3"
+SUB_ID 30 "Annexin VI H3"
+SUB_ID 31 "Annexin VI M3"
+SUB_ID 32 "Annexin IV H3"
+SUB_ID 33 "Annexin IV B3"
+SUB_ID 34 "Annexin IV P3"
+SUB_ID 35 "Annexin V BH3"
+SUB_ID 36 "Annexin III H3"
+SUB_ID 37 "Annexin III R3"
+SUB_ID 38 "Annexin VII 3"
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+F0
+ F4
+ C100
+ (R) 348 740 P
+F0
+ F5
+ C1
+ (I) 360 740 P
+F0
+ C100
+ (M) 372 740 P
+F5
+ C1
+ (V) 384 740 P
+F0
+ F1
+ C100
+ (S) 396 740 P
+F0
+ F4
+ (R) 408 740 P
+F0
+ C100
+ 96 750 moveto (1) show
+ 204 750 moveto (10) show
+ 324 750 moveto (20) show
+showpage
--- /dev/null
+# Illustration of ALSCRIPT Command File
+#
+# Illustrates some of the MASK commands
+#
+# example 1 # Comment lines begin with the # symbol
+#
+#
+# (A) Input and output files:
+#
+BLOCK_FILE example1.blc # Define the block file to format
+OUTPUT_FILE example4.ps # Where to put the result
+#
+MAX_SIDE 11 # Fiddle with margins...
+Y_OFFSET 50 # This is just to fill the page with the
+X_OFFSET 80 # Alignment. Defaults normally work OK
+#
+# (B) Page Layout and overall spacing:
+#
+ADD_SEQ 38 1 # Make space after sequence 38
+ADD_SEQ 39 3 # Make space after sequence 39
+ADD_SEQ 69 1
+PORTRAIT # Portrait paper orientation
+POINTSIZE 10 # 10 point default pointsize
+#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot
+IDENT_WIDTH 8
+#
+# (C) Font definitions:
+#
+DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize
+DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT
+DEFINE_FONT 4 Times-Bold DEFAULT
+DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT
+DEFINE_FONT 6 Times-Roman DEFAULT
+SETUP # Tell the program to get on with the formatting
+#
+# some formatting commands
+#
+SURROUND_CHARS LIV ALL # Surround all L and Y characters
+FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters
+FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps
+FONT_CHARS KR ALL 4 # Show K and R in Times-Bold
+BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16
+SHADE_CHARS + ALL 0.0 # Shade all + characters black
+SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40
+LINE TOP 2 40 8 # Draw horizontal lines to
+LINE BOTTOM 2 40 8 # Join up surrounded numbers
+TEXT 2 42 "Helix Pattern"
+TEXT 12 42 "Glycine Loop" # Add text annotations
+TEXT 21 42 "Helix Pattern"
+SUB_ID 49 "Helix Predictions"
+SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms
+SUB_ID 68 "Turn Predictions"
+#INVERSE_CHARS DE ALL
+#SHADE_CHARS D ALL 0.5
+#SHADE_CHARS E ALL 0.0
+#
+# (D) Some formatting commands: to illustrate the use of MASK
+#
+mask SETUP #setup the mask
+sub_chars 1 1 27 38 SPACE . #substitute spaces for dots
+mask ILLEGAL "." #. is not to be used in frequency calculation
+mask FRE 1 1 27 38 # do the frequency calculation
+mask BOX 1 1 27 38 # use the resulting mask to create boxing lines
+mask SHADE 1 1 27 38 0.2 # shade
+mask INVERSE 1 1 27 38 # inverse - could also alter the font
+sub_chars 1 1 27 38 . SPACE # switch the dots back to spaces
+#
+# (E) Now make the prediction histograms nicer.
+#
+SURROUND_CHARS H 1 44 27 53
+SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey
+SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H"
+#
+SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions
+SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions
+SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions
+#
+SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions
+SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions
+SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions
+#
+SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction
+SHADE_CHARS HY 1 75 27 75 0.5
+SHADE_CHARS T 1 75 27 75 0.9
+#
+#
+# Now change the identifier codes
+#
+ID_FONT ALL 6
+SUB_ID 1 "Annexin I H4"
+SUB_ID 2 "Annexin I M4"
+SUB_ID 3 "Annexin I R4"
+SUB_ID 4 "Annexin V R4"
+SUB_ID 5 "Annexin V H4"
+SUB_ID 6 "Annexin V C4"
+SUB_ID 7 "Annexin II H4"
+SUB_ID 8 "Annexin II B4"
+SUB_ID 9 "Annexin II M4"
+SUB_ID 10 "Annexin IV P4"
+SUB_ID 11 "Annexin IV B4"
+SUB_ID 12 "Annexin IV H4"
+SUB_ID 13 "Annexin VI H4"
+SUB_ID 14 "Annexin VI M4"
+SUB_ID 15 "Annexin VI H8"
+SUB_ID 16 "Annexin VI M8"
+SUB_ID 17 "Annexin V BH4"
+SUB_ID 18 "Annexin VII 4"
+SUB_ID 19 "Annexin III H4"
+SUB_ID 20 "Annexin III R4"
+SUB_ID 21 "Annexin II M3"
+SUB_ID 22 "Annexin II H3"
+SUB_ID 23 "Annexin II B3"
+SUB_ID 24 "Annexin I H3"
+SUB_ID 25 "Annexin I M3"
+SUB_ID 26 "Annexin I R3"
+SUB_ID 27 "Annexin V C3"
+SUB_ID 28 "Annexin V H3"
+SUB_ID 29 "Annexin V R3"
+SUB_ID 30 "Annexin VI H3"
+SUB_ID 31 "Annexin VI M3"
+SUB_ID 32 "Annexin IV H3"
+SUB_ID 33 "Annexin IV B3"
+SUB_ID 34 "Annexin IV P3"
+SUB_ID 35 "Annexin V BH3"
+SUB_ID 36 "Annexin III H3"
+SUB_ID 37 "Annexin III R3"
+SUB_ID 38 "Annexin VII 3"
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+#
+# This is the alscript command file used to create the figure
+# published in: Roach P. L. et al (1995), Nature (June)
+#
+# The figure shows Isopenicillin N synthase with homologues
+# secondary structure and other information. It makes use
+# of many features of alscript 2.0
+#
+# Comments in the file all start with the # sign
+#
+#
+# define the alignment block file
+BLOCK_FILE ipns.blc
+# define the output file name
+OUTPUT_FILE ipns.ps
+# select portrait mode - longest paper side is vertical
+PORTRAIT
+# select point size for characters - this can be any number.
+POINTSIZE 6.5
+#
+# Define fonts that we will use in the alignment. You give each font
+# a unique number. The names of the fonts are standard PostScript
+# font names.
+#
+# This one means call Helvetica at the Default pointsize, font 0
+DEFINE_FONT 0 Helvetica DEFAULT
+# This means call Helvetica-Oblique at 70% of the default point size, font 1
+DEFINE_FONT 1 Helvetica-Oblique REL 0.70
+DEFINE_FONT 2 Helvetica-Bold DEFAULT
+define_font 5 Symbol REL 1.2
+define_font 6 Helvetica REL 0.7
+#
+# Colour definitions
+#
+# Define the colours that we will use in the alignment. Each colour is given
+# a number by which it is referred to later. The three numbers following
+# each colour are red,green,blue intensities.
+#
+# e.g. this defines colour number 1 to be red (1,0,0).
+DEFINE_COLOUR 1 1 0 0
+#DEFINE_COLOUR 2 0.8 1 0.8
+DEFINE_COLOUR 2 1 1 0
+DEFINE_COLOUR 3 0 0 1
+DEFINE_COLOUR 4 1 1 1
+DEFINE_COLOUR 5 1 1 1
+DEFINE_COLOUR 6 1 0.5 0
+DEFINE_COLOUR 7 1 0 1
+DEFINE_COLOUR 8 0 0 0
+DEFINE_COLOUR 9 1 1 1
+define_colour 10 0 1 0
+define_colour 11 0 0 0
+#
+# The add_seq command adds extra space to the alignment you have read in.
+#
+#
+# Here we are adding space for four more lines after sequence number 8.
+add_seq 8 4
+#
+# This means don't number the sequences
+no_numbers
+#
+# Alter the width of the space for identifiers at the lhs of the plot. This
+# must be set by trial and error - 10 is the default.
+ident_width 6
+#
+# Only print sequence identifiers on the first line of a multi-line/page
+# alignment plot.
+id_only_on_first_line
+#
+# This is some fiddling to reduce the default vertical spacing between
+# sequence lines. We did this to improve the appearance of the alignment
+# in a single column of Nature. Other features of the display can
+# be modified in a similar way - e.g. x_space_factor.
+y_space_factor 0.05
+#
+# commands above the SETUP are about the overall layout etc. Commands
+# after, are giving specific boxing/shading/colouring etc.
+#
+SETUP
+#
+# Calcluate conservation values for the complete alignment. Conservation
+# values range from 0-10 where 10 is an identity. Values above 5 show
+# increasing similarities in physico-chemical properties. Having calculated
+# conservation values, we can then colour the plot to highlight conserved
+# positions.
+#
+calcons 1 1 424 8
+#
+# set mask for conserved position better than 5
+#
+# this clears the MASK array of all previous settings
+mask SETUP
+# this masks all columns of the alignment for which the conservation
+# is > 5.
+mask CONSERVATION ALL 5
+#
+# Now we box the masked positions and colour the
+# text backgrounds yellow (colour 2). Note that this operation could
+# be restricted to a subset of the alignment rather than the whole thing.
+mask BOX 1 1 424 8
+#
+# The SCOL command colours the backgrounds of the character. The CCOL
+# command colours the character itself.
+mask SCOL 1 1 424 8 2
+#
+# This resets the MASK commands ready for another operation. If you don't
+# reset, then subsequent mask commands are ADDED to the existing mask
+#
+mask RESET
+#
+#now do thw same for identities, only this time in red
+#with white lettering
+mask CONSERVATION ALL 10
+mask SCOL 1 1 424 8 1
+mask CCOL 1 1 424 8 99
+mask FONT 1 1 424 8 2
+mask RESET
+#
+# sub_id allows the identifier to be modified. At present it is not possible
+# to mix fonts within an ID, but the ID_FONT command can be used to modify the
+# font used for individual IDs.
+sub_id 1 "ACCO"
+sub_id 2 "F3OH"
+sub_id 3 "H6H "
+sub_id 4 "FOLS"
+sub_id 5 "GC20"
+sub_id 6 "DAOCS"
+sub_id 7 "DAOC/DACS "
+sub_id 8 "IPNS"
+#
+# These are the secondary structure definitions. They were entered by
+# hand from an analysis of the IPNS structure.
+#
+# Secondary structure
+#
+# Set Numbering relative to IPNS
+#
+# The x values (sequence position) of any commands that follow a
+# relative_to command will be translated into absolute alignment position.
+# This takes account of gaps in the alignment.
+relative_to 8
+#
+# Set the font for the line that will say alpha1, beta2 etc to Symbol font.
+# Region commands operate on a region of the alignment - they usually expect
+# four numbers to define the region - the numbers are x,y,x1,y1 where y and y1
+# are the sequences and x and x1 are the residue positions.
+font_region 1 11 331 11 5
+#
+#colour all secondary structures black (colour 11)
+#
+colour_text_region 1 10 331 10 11
+#
+# Now draw all the helices and strands and label and colour them.
+#
+# For example, Tell ALSCRIPT to draw a strand from 11 to 13 of sequence 10
+strand 11 10 13
+# Now write the text b1 (will be a Greek b because of the
+# font_region command above).
+ text 12 11 "b1"
+helix 15 10 18
+ text 16 11 "a1"
+helix 22 10 37
+ text 30 11 "a2"
+strand 41 10 45
+ text 43 11 "b2"
+helix 51 10 64
+ text 57 11 "a3"
+helix 67 10 73
+ text 70 11 "a4"
+strand 89 10 91
+ text 90 11 "b3"
+strand 101 10 105
+ text 103 11 "b4"
+helix 115 10 118
+ text 116 11 "a5"
+helix 138 10 163
+ text 151 11 "a6"
+helix 172 10 174
+ text 173 11 "a7"
+#
+#barrel strand 1
+strand 183 10 189
+# set the colour to green for this strand.
+ colour_text_region 183 10 189 10 10
+ text 186 11 "b5"
+#
+helix 197 10 199
+ text 198 11 "a8"
+strand 201 10 202
+ text 201 11 "b6"
+strand 208 10 209
+ text 208 11 "b7"
+#
+#barrel strands 2-6
+strand 210 10 214
+ text 212 11 "b8"
+ colour_text_region 210 10 214 10 10
+strand 220 10 225
+ text 222 11 "b9"
+ colour_text_region 220 10 225 10 10
+strand 231 10 235
+ text 233 11 "b10"
+ colour_text_region 231 10 235 10 10
+strand 238 10 241
+ text 239 11 "b11"
+ colour_text_region 238 10 241 10 10
+strand 249 10 253
+ text 250 11 "b12"
+ colour_text_region 249 10 253 10 10
+#
+helix 255 10 260
+ text 257 11 "a9"
+#
+#
+#barrel strands 7-8
+strand 270 10 273
+ text 271 11 "b13"
+ colour_text_region 270 10 273 10 10
+strand 279 10 286
+ text 282 11 "b14"
+ colour_text_region 279 10 286 10 10
+#
+#
+#
+strand 293 10 294
+ text 293 11 "b15"
+strand 311 10 312
+ text 311 11 "b16"
+helix 313 10 323
+ text 317 11 "a10"
+#
+#Now add arrows for the residues within 8A of the Mn
+#
+# The TEXT command normally just writes out the specified text
+# at the specified position. However, special TEXT objects
+# exist. These all start with the @ symbol.
+#
+colour_text_region 214 12 214 12 1
+#
+# This draws a filled up arrow at position 214,12.
+text 214 12 "@fuparrow"
+colour_text_region 216 12 216 12 1
+text 216 12 "@fuparrow"
+colour_text_region 270 12 270 12 1
+text 270 12 "@fuparrow"
+colour_text_region 330 12 330 12 1
+text 330 12 "@fuparrow"
+#
+text 41 12 "@fuparrow"
+text 252 12 "@fuparrow"
+text 221 12 "@fuparrow"
+text 187 12 "@fuparrow"
+text 285 12 "@fuparrow"
+text 223 12 "@fuparrow"
+text 272 12 "@fuparrow"
+text 218 12 "@fuparrow"
+text 231 12 "@fuparrow"
+#
+#
+# Now set the sequence numbering back to alignment positions.
+relative_to 0
+#
+#
+# From here to the end of the file was created by the alsnum program
+# alsnum creates text commands for numbering a plot relative to a
+# specific sequence, rather than the alignment numbers that are
+# normally shown. Please see the ALSCRIPT manual for further details.
+#
+#
+# Text commands created for numbering
+# Sequence Number: 8
+# Starting Number: 1
+# Interval: 10
+# Position: 9
+TEXT 53 9 "10"
+TEXT 65 9 "20"
+TEXT 78 9 "30"
+TEXT 88 9 "40"
+TEXT 101 9 "50"
+TEXT 111 9 "60"
+TEXT 121 9 "70"
+TEXT 131 9 "80"
+TEXT 141 9 "90"
+TEXT 151 9 "100"
+TEXT 161 9 "110"
+TEXT 171 9 "120"
+TEXT 181 9 "130"
+TEXT 191 9 "140"
+TEXT 201 9 "150"
+TEXT 211 9 "160"
+TEXT 221 9 "170"
+TEXT 231 9 "180"
+TEXT 241 9 "190"
+TEXT 251 9 "200"
+TEXT 261 9 "210"
+TEXT 271 9 "220"
+TEXT 281 9 "230"
+TEXT 297 9 "240"
+TEXT 307 9 "250"
+TEXT 317 9 "260"
+TEXT 327 9 "270"
+TEXT 343 9 "280"
+TEXT 353 9 "290"
+TEXT 363 9 "300"
+TEXT 378 9 "310"
+TEXT 388 9 "320"
+TEXT 398 9 "330"
--- /dev/null
+
+Conversion of CLUSTAL NBRF PIR file to AMPS BLOCKFILE format
+clus2blc: Geoffrey J. Barton (1992)
+
+>P1;3_LYCES 3_LYCES 418 bp PROT 0 JAN 1900, 418 bases, 1CE32436 checksum.
+>P1;U04434 U04434 428 bp PROT 0 JAN 1900, 428 bases, A5C97FB7 checksum.
+>P1;HY6H_HYONI HY6H_HYONI 406 bp PROT 0 JAN 1900, 406 bases, DF93DD1E checksum.
+>P1;S67953 S67953 400 bp PROT 0 JAN 1900, 400 bases, 1276A781 checksum.
+>P1;X73314 X73314 428 bp PROT 0 JAN 1900, 428 bases, 860E176 checksum.
+>P1;CEFE_STRCL CEFE_STRCL 402 bp PROT 0 JAN 1900, 402 bases, 97CFEE75 checksum.
+>P1;EXPA_CEPAC EXPA_CEPAC 422 bp PROT 0 JAN 1900, 422 bases, AE896850 checksum.
+>P1;IPNS_EMENI IPNS_EMENI 403 bp PROT 0 JAN 1900, 403 bases, 4DADFBBB checksum.
+* iteration 1
+ M
+ A
+ L
+ N
+ MG
+ KK
+ TV
+ M AA
+ A QT
+ P GE
+ V VS
+ S SA
+ I AP
+ S TS
+ A LN
+ V TL
+ P MN
+ F EE
+ LAVE
+ PTAM
+ TFRK
+ AVVG
+ ASQE
+ ENAY
+ GWIR
+ ESAP
+ TTSP
+ NKLF
+ VSSG
+ RVKG
+ ASCS
+ SEMD
+ FSDE
+ VFTS
+ RIIK
+ EAPV
+ EPSP
+ DLEE
+ EQYD
+ RKIF
+ PRRI
+ KASW
+ VEES
+ PKNE M
+ HDEK G
+ DVQF S
+ RPPE V
+ FVAA S
+MSGASDTK
+EDNTETSA
+NEDTLTKN
+FVVLLVVV
+PPPHPPPP
+IVIGVTVK
+IVIVLFFI
+NSDVDSRD
+LLLLVLLV
+ P
+ QT
+EEQVIADS
+KGQPDEDP
+LIHVLLLL
+ND IE F
+ G DK G
+ LF D
+ RM D
+ S
+ G
+ D
+GP DK Q
+DR PS A
+ER DYQKA
+RRHEVQSK
+AAHNEGGM
+NELKELKR
+TILMAHVV
+MRVVTQLA
+EGQKRDTQ
+MRQLLEEQ
+IVIIVFLI
+KATADRAD
+DAKDEREA
+AAAAACAA
+CCCSCLVS
+EEQKRRTR
+NDDEQDTD
+WWFWHKKT
+GGGGGGGG
+FILIILIF
+FFFFFFFF
+EQQQFYYY
+LVVLVLLA
+VVIIVTTV
+NDNNNDEN
+HHHHHCSH
+GGGGGGGG
+IVFIVLLI
+PDPPDTV
+HAEDIDD
+EAEEETD
+VLLAMEDN
+MVMIMLHV
+DALAGKTQ
+TDEDRSSR
+VMTLVAAL
+EAMQHKRS
+KREKDDEQ
+MLVVCLTK
+TACGMVCT
+KRKKNIVK
+GDEEEDDE
+HFFFFFFF
+YFFFFFFH
+KAAETEKM
+KLLHMHNS
+CPPVPGGI
+MPAPLSST
+EEEQDEEP
+QDEEVAEE
+RKKEKEEE
+FLEKQKKK
+KRKERRRW
+EFFLAAAD
+LDKIKVVL
+VMPARTTA
+ASKKKSLI
+SGGTVPAR
+KGEPGVDA
+ A Y
+ A N
+ K K
+ F E
+ E H
+ LG Q
+ PS PRD
+ LN TNQ
+ KEDEMAV
+ KQISRRR
+ GKEYRRA
+GGAGGGGG
+LFKYYFFY
+EILGTTSY
+AVYTNGAL
+ SVSSLLS
+ SELFEEI
+ G FSWP
+ E GEEG
+ RSSK
+ TTK
+ AAA
+ FQVV
+VHQQAIVE
+QLLKSTTS
+AQSENNEF
+EGNVLTTC
+VEEEPGGY
+TAEGWSKL
+DVFKKYYN
+LQLKESSP
+DDYGTDDN
+WWWWFYYF
+ERKVSSST
+SEDDLMTP
+TITHRCCD
+FVLLCYYH
+FTAFVSSP
+LYHHAMMR
+RFGKAGGI
+HSCIQTIQ
+LYHWNAGA
+ PPPSDGK
+ VLPSNNT
+PKDSA P
+TAQAA T
+SRDVH H
+NDLND E
+IYVYY V
+SSNRV N
+QRSYLLLV
+VWWWDFFW
+PPPPTPPP
+DDEKLSND
+LKKNGGRE
+DPPPPDGT
+ S K
+ F H
+EAAPS P
+EAKSH G
+YWYYHFFF
+RRRRGEEQ
+EAEEKRDD
+VVVAAIVF
+MVVNYWWA
+REAEQTQE
+DRKEEQDQ
+FYYYCYYY
+ASSGGFFY
+KEVKIDDW
+RQERARRD
+LLVMLQMV
+EMRRNYYF
+KAKEETGG
+LLLVLAAL
+AATVGSAS
+ECMDTRKS
+ERRRKADA
+LLMIIVVL
+LLLFVAAL
+DGDKERRK
+LVYSLEAG
+LLILLVVY
+CSCSGLLA
+EEELLRNL
+NAGGSASA
+LMLLLTVL
+GGGGGGGG
+LLLLITAK
+EDKESEPE
+KTLGRPLE
+GEGHEDAN
+YAYEY F
+LLFMF F
+KADIKGGA
+NRNENGER
+AAEAFVDH
+FCLA F
+YVSG K
+GD IP
+ EDD
+ ADD
+S GFFFT
+K DELVL
+G EDDEA
+PMQINCCS
+NDIVDEDV
+FQQYSPPV
+GKMLILLL
+TVMLLLLI
+KVLKRRRR
+VVTILFLY
+SNNNNRRP
+NFYYYYYY
+YYYYYFFL
+PPPPPPPD
+PRPPTQEP
+CCCCCVVY
+PPPPDPPP
+KQDRKEEE
+PPPPPHDA
+DDSDERRA
+LLSLVSVI
+ AAK
+ T
+ A
+ A
+ EED
+ EEG
+ QET
+ITTAVPPK
+KLLLLLLL
+GGGGGRRS
+LLSVTMMF
+RKGVGAGE
+ARGAPPPW
+HHHHHHHH
+TTYTTYYE
+DDDDDDDD
+APGMPLLV
+GGNSTSSS
+GTLYSMTL
+IIIIVVII
+ITTTTTTT
+LLLIILLV
+LLLLLIVL
+FLQVHQHY
+QQQPQQQQ
+DDDNDTTS
+DL EPPAN
+K CCV
+VVLVVAAQ
+SGPQSNNN
+GGGGGGG
+ FF
+ VV
+ SS
+LLLLLLLL
+QQQQQQQQ
+LAQVVACV
+LTLFCEEE
+KRIKSVVT
+DDVDNGDA
+EGKGDGGA
+ GD
+ RA
+QTTHQAEG
+WWWWWFFY
+IIIYYTVQ
+DTADSDDD
+VVVVILLI
+PQQKPPPE
+PPPYPYTA
+MVIINRLD
+REPPPPPD
+HGTNEDGT
+SAAAAAAG
+IFFLFVMY
+VVVIVLVL
+VVVVIVVI
+NNNHNFFN
+LLLIICCC
+GGGGGGGG
+DDLDDAAS
+QHTQTIVY
+LGLVFAGM
+EHKETTTA
+VLVISLLH
+ILILLVAL
+TSTSTTTT
+NNNNNGGN
+GGEGGGGN
+KRKKIQKY
+YFFYYVVY
+KKEKKKKK
+SNGSGAAA
+VASVCPPP
+LDIYIRKI
+HHHHHHHH
+RQRRRHRR
+VAVTAVVV
+IVVTVAKK
+AVTVVAS
+ PP
+ RG
+ RR
+ DD
+QNDNNQQ
+TSPKSIRW
+DETDMAVV
+GCRKNGGN
+TSDTASSA
+ SSE
+RRRRRRRR
+MLVMKTTQ
+SSSSSSSS
+LIIWLSSL
+AAAPAVVP
+STTVFFFF
+FFLFFFFF
+YQILLLLV
+NNGECRRN
+PPPPPPPL
+GADPSNKG
+SPYSHAPY
+DDSEDDDD
+AACHKFFS
+VTTEVTSV
+IVIVVFFI
+YYEGRSND
+PPPPAVVP
+ALAIPPQF
+KAKPELQD
+T EKEASP
+LVLLLRRR
+VRLLVEEE
+EDNSECWP
+KGQEKGGN
+EEDASFFG
+AANNPDNK
+EPPPPVVS
+EIP SR
+ LL LI
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+-0.84 41.28 -0.84 35.28 6.16 35.28 6.16 41.28 -0.84 41.28 ML
+closepath C99 fill
+ newpath
+-0.84 35.28 -0.84 29.28 6.16 29.28 6.16 35.28 -0.84 35.28 ML
+closepath C99 fill
+ newpath
+-0.84 29.28 -0.84 23.28 6.16 23.28 6.16 29.28 -0.84 29.28 ML
+closepath C99 fill
+ newpath
+-0.84 23.28 -0.84 17.28 6.16 17.28 6.16 23.28 -0.84 23.28 ML
+closepath C99 fill
+ newpath
+-0.84 17.28 -0.84 11.28 6.16 11.28 6.16 17.28 -0.84 17.28 ML
+closepath C99 fill
+ newpath
+-0.84 11.28 -0.84 5.28 6.16 5.28 6.16 11.28 -0.84 11.28 ML
+closepath C99 fill
+ newpath
+-0.84 5.28 -0.84 -0.72 6.16 -0.72 6.16 5.28 -0.84 5.28 ML
+closepath C99 fill
+C100
+ C1
+ 49.00 0.00 moveto
+56.00 0.00 lineto
+52.50 5.40 lineto
+49.00 0.00 lineto
+stroke
+49.00 0.00 moveto
+56.00 0.00 lineto
+52.50 5.40 lineto
+49.00 0.00 lineto
+closepath
+ C1
+ fill
+C100
+ C11
+ 3.50 12.00 moveto
+3.50 17.40 lineto
+0.00 17.40 lineto
+0.00 12.00 lineto
+3.50 12.00 lineto
+stroke
+3.50 12.00 moveto
+3.50 17.40 lineto
+0.00 17.40 lineto
+0.00 12.00 lineto
+3.50 12.00 lineto
+closepath
+ C11
+ fill
+newpath
+3.50 12.00 moveto
+1.40 12.00 1.40 17.40 3.50 17.40 curveto
+5.60 17.40 5.60 12.00 3.50 12.00 curveto
+stroke
+newpath
+3.50 12.00 moveto
+1.40 12.00 1.40 17.40 3.50 17.40 curveto
+5.60 17.40 5.60 12.00 3.50 12.00 curveto
+closepath
+ C11
+ fill
+3.50 14.43 moveto
+3.50 14.97 lineto
+7.00 14.97 lineto
+7.00 14.43 lineto
+3.50 14.43 lineto
+stroke
+3.50 14.43 moveto
+3.50 14.97 lineto
+7.00 14.97 lineto
+7.00 14.43 lineto
+3.50 14.43 lineto
+closepath
+ C11
+ fill
+C100
+ 49 18 moveto (330) show
+(S) 0 24 P
+(L) 7 24 P
+(I) 14 24 P
+(N) 21 24 P
+(K) 28 24 P
+(N) 35 24 P
+(G) 42 24 P
+(Q) 49 24 P
+(T) 56 24 P
+(N) 0 30 P
+(M) 7 30 P
+(R) 14 30 P
+(R) 21 30 P
+(D) 28 30 P
+(K) 35 30 P
+(P) 42 30 P
+(A) 49 30 P
+(A) 56 30 P
+(A) 63 30 P
+(E) 70 30 P
+(A) 77 30 P
+(A) 84 30 P
+(V) 91 30 P
+(P) 98 30 P
+(A) 105 30 P
+(A) 112 30 P
+(A) 119 30 P
+(P) 126 30 P
+(V) 133 30 P
+(S) 140 30 P
+(T) 147 30 P
+(A) 154 30 P
+(A) 161 30 P
+(P) 168 30 P
+(I) 175 30 P
+(A) 182 30 P
+(T) 189 30 P
+(N) 0 36 P
+(I) 7 36 P
+(R) 14 36 P
+(R) 21 36 P
+(T) 28 36 P
+(S) 35 36 P
+(K) 42 36 P
+(A) 49 36 P
+(R) 0 42 P
+(Y) 7 42 P
+(R) 14 42 P
+(P) 21 42 P
+(D) 28 42 P
+(C) 35 42 P
+(N) 42 42 P
+(T) 49 42 P
+(L) 56 42 P
+(E) 63 42 P
+(A) 70 42 P
+(F) 77 42 P
+(K) 84 42 P
+(T) 91 42 P
+(W) 98 42 P
+(V) 105 42 P
+(Q) 112 42 P
+(E) 119 42 P
+(G) 126 42 P
+(K) 133 42 P
+(A) 140 42 P
+(L) 147 42 P
+(D) 154 42 P
+(T) 161 42 P
+(G) 168 42 P
+(S) 175 42 P
+(T) 182 42 P
+(I) 189 42 P
+(T) 196 42 P
+(A) 203 42 P
+(P) 210 42 P
+(S) 217 42 P
+(A) 224 42 P
+(K) 0 48 P
+(L) 7 48 P
+(P) 14 48 P
+(Q) 21 48 P
+(Y) 0 54 P
+(D) 7 54 P
+(S) 14 54 P
+(G) 21 54 P
+(V) 28 54 P
+(K) 35 54 P
+(P) 42 54 P
+(Y) 49 54 P
+(K) 56 54 P
+(I) 63 54 P
+(N) 70 54 P
+(V) 77 54 P
+(L) 0 60 P
+(A) 7 60 P
+(R) 14 60 P
+(L) 21 60 P
+(K) 28 60 P
+(K) 35 60 P
+(Q) 42 60 P
+(A) 49 60 P
+(K) 56 60 P
+(A) 63 60 P
+(D) 70 60 P
+(K) 77 60 P
+(K) 84 60 P
+(Q) 91 60 P
+(Q) 98 60 P
+(Q) 105 60 P
+(Q) 112 60 P
+(S) 119 60 P
+(A) 126 60 P
+(N) 133 60 P
+(K) 140 60 P
+(E) 147 60 P
+(F) 154 60 P
+(A) 161 60 P
+(D) 168 60 P
+(S) 175 60 P
+(K) 182 60 P
+(P) 189 60 P
+(L) 196 60 P
+(D) 203 60 P
+(A) 210 60 P
+(I) 217 60 P
+(F) 224 60 P
+(A) 231 60 P
+(L) 0 66 P
+(K) 7 66 P
+(F) 14 66 P
+(Q) 21 66 P
+(A) 28 66 P
+(K) 35 66 P
+(E) 42 66 P
+(P) 49 66 P
+(R) 56 66 P
+(F) 63 66 P
+(E) 70 66 P
+(A) 77 66 P
+(M) 84 66 P
+(K) 91 66 P
+(A) 98 66 P
+(M) 105 66 P
+(E) 112 66 P
+(S) 119 66 P
+(D) 126 66 P
+(P) 133 66 P
+(I) 140 66 P
+(A) 147 66 P
+(S) 154 66 P
+(A) 161 66 P
+0 -168 translate
+0 84 translate
+2 setlinecap
+ 0.33 setlinewidth
+showpage
--- /dev/null
+#Comments in ALscript command files start with a #
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+SETUP #Tell the program to get on with the formatting.
+
--- /dev/null
+A simple example block file. The important parts are the lines that
+start with the greater than character - these are identifer lines for the
+sequences. The first star is above the position of the first sequence
+in the alingmnet which is written VERTICALLY down the page. The second
+star shows the end of the alignment.
+
+Anything else can be written here, except greater than signs and stars.
+
+This is the format produced by the AMPS package for multiple
+sequence alignment.
+
+
+>one
+>two
+>three
+>four
+>five
+*
+ABCDE
+EDFTD
+AAAAA
+LLL
+PPPPP
+GGGGG
+SSSSS
+KLMNT
+TTLPQ
+SLNST
+TTLSN
+LQPPL
+LLLLL
+TTTTT
+*
--- /dev/null
+#SILENT_MODE
+# Illustration of ALSCRIPT Command File
+#
+# As shown in: Barton, G. J. (1992)
+# ALSCRIPT- A tool to format multiple sequence alignments
+# Protein Engineering (in press)
+#
+# example 1 # Comment lines begin with the # symbol
+#
+#
+# (A) Input and output files:
+#
+BLOCK_FILE example1.blc # Define the block file to format
+OUTPUT_FILE example1.ps # Where to put the result
+#
+MAX_SIDE 11 # Fiddle with margins...
+Y_OFFSET 50 # This is just to fill the page with the
+X_OFFSET 80 # Alignment. Defaults normally work OK
+#
+# (B) Page Layout and overall spacing:
+#
+ADD_SEQ 38 1 # Make space after sequence 38
+ADD_SEQ 39 3 # Make space after sequence 39
+ADD_SEQ 69 1
+PORTRAIT # Portrait paper orientation
+POINTSIZE 10 # 10 point default pointsize
+#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot
+IDENT_WIDTH 8
+#
+# (C) Font definitions:
+#
+DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize
+DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT
+DEFINE_FONT 4 Times-Bold DEFAULT
+DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT
+DEFINE_FONT 6 Times-Roman DEFAULT
+SETUP # Tell the program to get on with the formatting
+#
+# (D) Some formatting commands:
+#
+SURROUND_CHARS LIV ALL # Surround all L and Y characters
+FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters
+FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps
+FONT_CHARS KR ALL 4 # Show K and R in Times-Bold
+BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16
+SHADE_CHARS + ALL 0.0 # Shade all + characters black
+SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40
+LINE TOP 2 40 8 # Draw horizontal lines to
+LINE BOTTOM 2 40 8 # Join up surrounded numbers
+TEXT 2 42 "Helix Pattern"
+TEXT 12 42 "Glycine Loop" # Add text annotations
+TEXT 21 42 "Helix Pattern"
+SUB_ID 49 "Helix Predictions"
+SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms
+SUB_ID 68 "Turn Predictions"
+INVERSE_CHARS DE ALL
+SHADE_CHARS D ALL 0.5
+SHADE_CHARS E ALL 0.0
+#
+# (E) Now make the prediction histograms nicer.
+#
+SURROUND_CHARS H 1 44 27 53
+SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey
+SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H"
+#
+SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions
+SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions
+SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions
+#
+SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions
+SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions
+SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions
+#
+SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction
+SHADE_CHARS HY 1 75 27 75 0.5
+SHADE_CHARS T 1 75 27 75 0.9
+#
+#
+# Now change the identifier codes
+#
+ID_FONT ALL 6
+SUB_ID 1 "Annexin I H4"
+SUB_ID 2 "Annexin I M4"
+SUB_ID 3 "Annexin I R4"
+SUB_ID 4 "Annexin V R4"
+SUB_ID 5 "Annexin V H4"
+SUB_ID 6 "Annexin V C4"
+SUB_ID 7 "Annexin II H4"
+SUB_ID 8 "Annexin II B4"
+SUB_ID 9 "Annexin II M4"
+SUB_ID 10 "Annexin IV P4"
+SUB_ID 11 "Annexin IV B4"
+SUB_ID 12 "Annexin IV H4"
+SUB_ID 13 "Annexin VI H4"
+SUB_ID 14 "Annexin VI M4"
+SUB_ID 15 "Annexin VI H8"
+SUB_ID 16 "Annexin VI M8"
+SUB_ID 17 "Annexin V BH4"
+SUB_ID 18 "Annexin VII 4"
+SUB_ID 19 "Annexin III H4"
+SUB_ID 20 "Annexin III R4"
+SUB_ID 21 "Annexin II M3"
+SUB_ID 22 "Annexin II H3"
+SUB_ID 23 "Annexin II B3"
+SUB_ID 24 "Annexin I H3"
+SUB_ID 25 "Annexin I M3"
+SUB_ID 26 "Annexin I R3"
+SUB_ID 27 "Annexin V C3"
+SUB_ID 28 "Annexin V H3"
+SUB_ID 29 "Annexin V R3"
+SUB_ID 30 "Annexin VI H3"
+SUB_ID 31 "Annexin VI M3"
+SUB_ID 32 "Annexin IV H3"
+SUB_ID 33 "Annexin IV B3"
+SUB_ID 34 "Annexin IV P3"
+SUB_ID 35 "Annexin V BH3"
+SUB_ID 36 "Annexin III H3"
+SUB_ID 37 "Annexin III R3"
+SUB_ID 38 "Annexin VII 3"
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+>L1H4
+>L1M4
+>L1R4
+>L5R4
+>L5H4
+>L5C4
+>L2H4
+>L2B4
+>L2M4
+>L4P4
+>L4B4
+>L4H4
+>L6H4
+>L6M4
+>L6H8
+>L6M8
+>L5BH4
+>p474
+>L3H4
+>L3R4
+>L2M3
+>L2H3
+>L2B3
+>L1H3
+>L1M3
+>L1R3
+>L5C3
+>L5H3
+>L5R3
+>L6H3
+>L6M3
+>L4H3
+>L4B3
+>L4P3
+>L5BH3
+>L3H3
+>L3R3
+>p473
+>Conservation
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+>
+ *
+ FFFLLFFFFFFFFFFFFFLLDDDDDDDDDDDDDDDDDD HHHHHHH E TTTTH
+ AAAAAAAAAAAAAAAAAAAAAAAAAAAAAVVAAAAAAA7 HHHHHHHH TTTH
+ EEEEEEDDDEEEEEDDEEEGRRRRRRQQQQQQQQQQQQ HHHHHHHH TTH
+ KKKTTTRRRRRKRRKKRRRREDDAAAVAADDDDDDITR HHHHHHHHH E TTTH
+ LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLL+ HHHHHHHHH E TTTH
+ HYYYYYYYYYYYFFYYYYHHYYYYYYFFFYYYYYYYYY HHHHHHHHH E TTH
+ QEEYYYDDDKKKKKKKYYRQDDDEEERQQEEEEEAKDQ HHHHHHHHH E TTH
+ AAAAASSSSSSSAASSAAAAAAAAAAAAAAAAAAAAAA7 HHHHHHHH E TTH
+ GGGGGGGGGGGGGGGGGG H T
+ MMMMMMMMMMMMMMMMMMLLVVVEEEEEEEEEEEEEEE HHHHHHHH E TTTH
+ KKKKKKKKKKKKKKKKKKKKKKKRRRLLLLLKKKKNKG HHHHHHH E TTTTH
+ GGGGGGGGGGGGGGGGGGGGRRRRRRKKKKKKKKIRKR HHH TTTTT
+ VAAAAAKKKLLLLLAAAAIAKKKKKKWWWWWWWWRWWL HHH TTTTT
+ GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG+ HH TTTTTTT
+ TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5 HHHH TTTTTTTTTT
+ RRRDDDRRRDDDRRDDRDDDDDDDDDDDDDDDDDDDDD HHHHH TTTTTTTTTT
+ HHHDDDDDDDDDDDEEDDEEVVVVVVEEEEEEEEEEEE HHHHHHHH E TTTTTTTTTT
+ KKKHHDKKKNDNNNKKGSFFPPPNNNEEEAAVVVMDDS HHHHHHH E TTTTTTTY
+ AATTTTVVVTTTTTTTTTTTKKKVVVTKKQQKKKKKKC HHHHHHHH EEE TTTTTH
+ LLLLLLLLLLLLLLLLLLLLWWWFFFFFFFFFFFFFFF6 HHHHHHHH EEEE TTH
+ IIIIIIIIIIIIIITTIVNNIIINTNIIIIILLLITTN5 HHHHHHH EEEE TH
+ RRRRRRRRRRRRRRRRRRRRSSSTTTTTTYYTTTTEEM HHHHHHH EEEEE H
+ IIIVVVIIIVVVIIIVNIIIIIIIIIIIIIIVVVIIII5 HHHHHH EEEEE H
+ MMMIMMMMMMMMMMMMIVMMMMMLLLLFLLLLLLLLLL6 HHHHHH EEEEE TH
+ VVVVVVVVVVVVVVVVVVVVTTTTTTGGGGGCCCCCCA6 HHHHH EEEE TTTTH
+ SSSSSSSSSSSSSSSSSTSSEEETSTTTTNNSSSTLLT HHHH EE TTTTTT
+ RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR+ HHHH EE TTTTTTTTTT
+ *
+
+
+
--- /dev/null
+# FILE example2.com
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE example.blc #define the block file to format
+OUTPUT_FILE example2.ps #where to put the result
+LANDSCAPE #landscape paper orientation
+POINTSIZE 12 #12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica
+DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica
+DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic
+NUMBER_SEQS #Number the sequences at left hand side
+SETUP #Tell the program to get on with the formatting.
+#
+#step 2 commands come after the SETUP command
+#
+#Here are some examples...
+#
+SURROUND_CHARS GP ALL #draw lines around all G and P
+SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6
+BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20
+FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters
+ID_FONT ALL 1 #set identifiers in font 1
--- /dev/null
+# Illustration of ALSCRIPT Command File
+#
+# As shown in: Barton, G. J. (1992)
+# ALSCRIPT- A tool to format multiple sequence alignments
+# Protein Engineering (in press)
+#
+# Modified version - includes colour commands
+#
+#
+# example 1 # Comment lines begin with the # symbol
+#
+#
+# (A) Input and output files:
+#
+BLOCK_FILE example1.blc # Define the block file to format
+OUTPUT_FILE example3.ps # Where to put the result
+#
+MAX_SIDE 11 # Fiddle with margins...
+Y_OFFSET 50 # This is just to fill the page with the
+X_OFFSET 80 # Alignment. Defaults normally work OK
+#
+# (B) Page Layout and overall spacing:
+#
+ADD_SEQ 38 1 # Make space after sequence 38
+ADD_SEQ 39 3 # Make space after sequence 39
+ADD_SEQ 69 1
+PORTRAIT # Portrait paper orientation
+POINTSIZE 10 # 10 point default pointsize
+#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot
+IDENT_WIDTH 8
+#
+# (C) Font definitions:
+#
+DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize
+DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT
+DEFINE_FONT 4 Times-Bold DEFAULT
+DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT
+DEFINE_FONT 6 Times-Roman DEFAULT
+#
+# COLOUR DEFINITIONS give a colour number, then red, green, blue intensities
+#
+# red
+DEFINE_COLOUR 0 1 0 0
+# green
+DEFINE_COLOUR 1 0 1 0
+# blue
+DEFINE_COLOUR 2 0 0 1
+# yellow
+DEFINE_COLOUR 3 1 1 0
+# magenta
+DEFINE_COLOUR 4 1 0 1
+# cyan
+DEFINE_COLOUR 5 0 1 1
+#
+DEFINE_COLOUR 6 0.7 0.3 0
+#single_page
+#background_colour 100
+SETUP # Tell the program to get on with the formatting
+#background_region -10 -10 468 792
+#
+# (D) Some formatting commands:
+#
+SURROUND_CHARS LIV ALL # Surround all L and Y characters
+FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters
+FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps
+FONT_CHARS KR ALL 4 # Show K and R in Times-Bold
+Ccol_CHARS LIV ALL 1
+Scol_CHARS ASN ALL 0
+BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16
+SHADE_CHARS + ALL 0.0 # Shade all + characters black
+COLOUR_REGION 14 1 16 38 2
+SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40
+LINE TOP 2 40 8 # Draw horizontal lines to
+LINE BOTTOM 2 40 8 # Join up surrounded numbers
+TEXT 2 42 "Helix Pattern"
+TEXT 12 42 "Glycine Loop" # Add text annotations
+TEXT 21 42 "Helix Pattern"
+SUB_ID 49 "Helix Predictions"
+SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms
+SUB_ID 68 "Turn Predictions"
+INVERSE_CHARS DE ALL
+SHADE_CHARS D ALL 0.5
+SHADE_CHARS E ALL 0.0
+#
+# (E) Now make the prediction histograms nicer.
+#
+SURROUND_CHARS H 1 44 27 53
+Scol_CHARS H 1 44 27 53 3 # Shade the Helix predictions 0.5 grey
+SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H"
+#
+SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions
+Scol_CHARS E 1 54 27 63 4 # Colour beta blue
+SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions
+#
+SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions
+Scol_CHARS T 1 64 27 73 6 # Shade the Turn Predictions
+SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions
+#
+SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction
+SHADE_CHARS HY 1 75 27 75 0.5
+SHADE_CHARS T 1 75 27 75 0.9
+#
+#
+# Now change the identifier codes
+#
+ID_FONT ALL 6
+SUB_ID 1 "Annexin I H4"
+SUB_ID 2 "Annexin I M4"
+SUB_ID 3 "Annexin I R4"
+SUB_ID 4 "Annexin V R4"
+SUB_ID 5 "Annexin V H4"
+SUB_ID 6 "Annexin V C4"
+SUB_ID 7 "Annexin II H4"
+SUB_ID 8 "Annexin II B4"
+SUB_ID 9 "Annexin II M4"
+SUB_ID 10 "Annexin IV P4"
+SUB_ID 11 "Annexin IV B4"
+SUB_ID 12 "Annexin IV H4"
+SUB_ID 13 "Annexin VI H4"
+SUB_ID 14 "Annexin VI M4"
+SUB_ID 15 "Annexin VI H8"
+SUB_ID 16 "Annexin VI M8"
+SUB_ID 17 "Annexin V BH4"
+SUB_ID 18 "Annexin VII 4"
+SUB_ID 19 "Annexin III H4"
+SUB_ID 20 "Annexin III R4"
+SUB_ID 21 "Annexin II M3"
+SUB_ID 22 "Annexin II H3"
+SUB_ID 23 "Annexin II B3"
+SUB_ID 24 "Annexin I H3"
+SUB_ID 25 "Annexin I M3"
+SUB_ID 26 "Annexin I R3"
+SUB_ID 27 "Annexin V C3"
+SUB_ID 28 "Annexin V H3"
+SUB_ID 29 "Annexin V R3"
+SUB_ID 30 "Annexin VI H3"
+SUB_ID 31 "Annexin VI M3"
+SUB_ID 32 "Annexin IV H3"
+SUB_ID 33 "Annexin IV B3"
+SUB_ID 34 "Annexin IV P3"
+SUB_ID 35 "Annexin V BH3"
+SUB_ID 36 "Annexin III H3"
+SUB_ID 37 "Annexin III R3"
+SUB_ID 38 "Annexin VII 3"
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+# Illustration of ALSCRIPT Command File
+#
+# Illustrates some of the MASK commands
+#
+# example 1 # Comment lines begin with the # symbol
+#
+#
+# (A) Input and output files:
+#
+BLOCK_FILE example1.blc # Define the block file to format
+OUTPUT_FILE example4.ps # Where to put the result
+#
+MAX_SIDE 11 # Fiddle with margins...
+Y_OFFSET 50 # This is just to fill the page with the
+X_OFFSET 80 # Alignment. Defaults normally work OK
+#
+# (B) Page Layout and overall spacing:
+#
+ADD_SEQ 38 1 # Make space after sequence 38
+ADD_SEQ 39 3 # Make space after sequence 39
+ADD_SEQ 69 1
+PORTRAIT # Portrait paper orientation
+POINTSIZE 10 # 10 point default pointsize
+#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot
+IDENT_WIDTH 8
+#
+# (C) Font definitions:
+#
+DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize
+DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT
+DEFINE_FONT 4 Times-Bold DEFAULT
+DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT
+DEFINE_FONT 6 Times-Roman DEFAULT
+SETUP # Tell the program to get on with the formatting
+#
+# some formatting commands
+#
+SURROUND_CHARS LIV ALL # Surround all L and Y characters
+FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters
+FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps
+FONT_CHARS KR ALL 4 # Show K and R in Times-Bold
+BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16
+SHADE_CHARS + ALL 0.0 # Shade all + characters black
+SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40
+LINE TOP 2 40 8 # Draw horizontal lines to
+LINE BOTTOM 2 40 8 # Join up surrounded numbers
+TEXT 2 42 "Helix Pattern"
+TEXT 12 42 "Glycine Loop" # Add text annotations
+TEXT 21 42 "Helix Pattern"
+SUB_ID 49 "Helix Predictions"
+SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms
+SUB_ID 68 "Turn Predictions"
+#INVERSE_CHARS DE ALL
+#SHADE_CHARS D ALL 0.5
+#SHADE_CHARS E ALL 0.0
+#
+# (D) Some formatting commands: to illustrate the use of MASK
+#
+mask SETUP #setup the mask
+sub_chars 1 1 27 38 SPACE . #substitute spaces for dots
+mask ILLEGAL "." #. is not to be used in frequency calculation
+mask FRE 1 1 27 38 # do the frequency calculation
+mask BOX 1 1 27 38 # use the resulting mask to create boxing lines
+mask SHADE 1 1 27 38 0.2 # shade
+mask INVERSE 1 1 27 38 # inverse - could also alter the font
+sub_chars 1 1 27 38 . SPACE # switch the dots back to spaces
+#
+# (E) Now make the prediction histograms nicer.
+#
+SURROUND_CHARS H 1 44 27 53
+SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey
+SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H"
+#
+SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions
+SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions
+SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions
+#
+SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions
+SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions
+SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions
+#
+SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction
+SHADE_CHARS HY 1 75 27 75 0.5
+SHADE_CHARS T 1 75 27 75 0.9
+#
+#
+# Now change the identifier codes
+#
+ID_FONT ALL 6
+SUB_ID 1 "Annexin I H4"
+SUB_ID 2 "Annexin I M4"
+SUB_ID 3 "Annexin I R4"
+SUB_ID 4 "Annexin V R4"
+SUB_ID 5 "Annexin V H4"
+SUB_ID 6 "Annexin V C4"
+SUB_ID 7 "Annexin II H4"
+SUB_ID 8 "Annexin II B4"
+SUB_ID 9 "Annexin II M4"
+SUB_ID 10 "Annexin IV P4"
+SUB_ID 11 "Annexin IV B4"
+SUB_ID 12 "Annexin IV H4"
+SUB_ID 13 "Annexin VI H4"
+SUB_ID 14 "Annexin VI M4"
+SUB_ID 15 "Annexin VI H8"
+SUB_ID 16 "Annexin VI M8"
+SUB_ID 17 "Annexin V BH4"
+SUB_ID 18 "Annexin VII 4"
+SUB_ID 19 "Annexin III H4"
+SUB_ID 20 "Annexin III R4"
+SUB_ID 21 "Annexin II M3"
+SUB_ID 22 "Annexin II H3"
+SUB_ID 23 "Annexin II B3"
+SUB_ID 24 "Annexin I H3"
+SUB_ID 25 "Annexin I M3"
+SUB_ID 26 "Annexin I R3"
+SUB_ID 27 "Annexin V C3"
+SUB_ID 28 "Annexin V H3"
+SUB_ID 29 "Annexin V R3"
+SUB_ID 30 "Annexin VI H3"
+SUB_ID 31 "Annexin VI M3"
+SUB_ID 32 "Annexin IV H3"
+SUB_ID 33 "Annexin IV B3"
+SUB_ID 34 "Annexin IV P3"
+SUB_ID 35 "Annexin V BH3"
+SUB_ID 36 "Annexin III H3"
+SUB_ID 37 "Annexin III R3"
+SUB_ID 38 "Annexin VII 3"
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+#
+# This is the alscript command file used to create the figure
+# published in: Roach P. L. et al (1995), Nature (June)
+#
+# The figure shows Isopenicillin N synthase with homologues
+# secondary structure and other information. It makes use
+# of many features of alscript 2.0
+#
+# Comments in the file all start with the # sign
+#
+#
+# define the alignment block file
+BLOCK_FILE ipns.blc
+# define the output file name
+OUTPUT_FILE ipns.ps
+# select portrait mode - longest paper side is vertical
+PORTRAIT
+# select point size for characters - this can be any number.
+POINTSIZE 6.5
+#
+# Define fonts that we will use in the alignment. You give each font
+# a unique number. The names of the fonts are standard PostScript
+# font names.
+#
+# This one means call Helvetica at the Default pointsize, font 0
+DEFINE_FONT 0 Helvetica DEFAULT
+# This means call Helvetica-Oblique at 70% of the default point size, font 1
+DEFINE_FONT 1 Helvetica-Oblique REL 0.70
+DEFINE_FONT 2 Helvetica-Bold DEFAULT
+define_font 5 Symbol REL 1.2
+define_font 6 Helvetica REL 0.7
+#
+# Colour definitions
+#
+# Define the colours that we will use in the alignment. Each colour is given
+# a number by which it is referred to later. The three numbers following
+# each colour are red,green,blue intensities.
+#
+# e.g. this defines colour number 1 to be red (1,0,0).
+DEFINE_COLOUR 1 1 0 0
+#DEFINE_COLOUR 2 0.8 1 0.8
+DEFINE_COLOUR 2 1 1 0
+DEFINE_COLOUR 3 0 0 1
+DEFINE_COLOUR 4 1 1 1
+DEFINE_COLOUR 5 1 1 1
+DEFINE_COLOUR 6 1 0.5 0
+DEFINE_COLOUR 7 1 0 1
+DEFINE_COLOUR 8 0 0 0
+DEFINE_COLOUR 9 1 1 1
+define_colour 10 0 1 0
+define_colour 11 0 0 0
+#
+# The add_seq command adds extra space to the alignment you have read in.
+#
+#
+# Here we are adding space for four more lines after sequence number 8.
+add_seq 8 4
+#
+# This means don't number the sequences
+no_numbers
+#
+# Alter the width of the space for identifiers at the lhs of the plot. This
+# must be set by trial and error - 10 is the default.
+ident_width 6
+#
+# Only print sequence identifiers on the first line of a multi-line/page
+# alignment plot.
+id_only_on_first_line
+#
+# This is some fiddling to reduce the default vertical spacing between
+# sequence lines. We did this to improve the appearance of the alignment
+# in a single column of Nature. Other features of the display can
+# be modified in a similar way - e.g. x_space_factor.
+y_space_factor 0.05
+#
+# commands above the SETUP are about the overall layout etc. Commands
+# after, are giving specific boxing/shading/colouring etc.
+#
+SETUP
+#
+# Calcluate conservation values for the complete alignment. Conservation
+# values range from 0-10 where 10 is an identity. Values above 5 show
+# increasing similarities in physico-chemical properties. Having calculated
+# conservation values, we can then colour the plot to highlight conserved
+# positions.
+#
+calcons 1 1 424 8
+#
+# set mask for conserved position better than 5
+#
+# this clears the MASK array of all previous settings
+mask SETUP
+# this masks all columns of the alignment for which the conservation
+# is > 5.
+mask CONSERVATION ALL 5
+#
+# Now we box the masked positions and colour the
+# text backgrounds yellow (colour 2). Note that this operation could
+# be restricted to a subset of the alignment rather than the whole thing.
+mask BOX 1 1 424 8
+#
+# The SCOL command colours the backgrounds of the character. The CCOL
+# command colours the character itself.
+mask SCOL 1 1 424 8 2
+#
+# This resets the MASK commands ready for another operation. If you don't
+# reset, then subsequent mask commands are ADDED to the existing mask
+#
+mask RESET
+#
+#now do thw same for identities, only this time in red
+#with white lettering
+mask CONSERVATION ALL 10
+mask SCOL 1 1 424 8 1
+mask CCOL 1 1 424 8 99
+mask FONT 1 1 424 8 2
+mask RESET
+#
+# sub_id allows the identifier to be modified. At present it is not possible
+# to mix fonts within an ID, but the ID_FONT command can be used to modify the
+# font used for individual IDs.
+sub_id 1 "ACCO"
+sub_id 2 "F3OH"
+sub_id 3 "H6H "
+sub_id 4 "FOLS"
+sub_id 5 "GC20"
+sub_id 6 "DAOCS"
+sub_id 7 "DAOC/DACS "
+sub_id 8 "IPNS"
+#
+# These are the secondary structure definitions. They were entered by
+# hand from an analysis of the IPNS structure.
+#
+# Secondary structure
+#
+# Set Numbering relative to IPNS
+#
+# The x values (sequence position) of any commands that follow a
+# relative_to command will be translated into absolute alignment position.
+# This takes account of gaps in the alignment.
+relative_to 8
+#
+# Set the font for the line that will say alpha1, beta2 etc to Symbol font.
+# Region commands operate on a region of the alignment - they usually expect
+# four numbers to define the region - the numbers are x,y,x1,y1 where y and y1
+# are the sequences and x and x1 are the residue positions.
+font_region 1 11 331 11 5
+#
+#colour all secondary structures black (colour 11)
+#
+colour_text_region 1 10 331 10 11
+#
+# Now draw all the helices and strands and label and colour them.
+#
+# For example, Tell ALSCRIPT to draw a strand from 11 to 13 of sequence 10
+strand 11 10 13
+# Now write the text b1 (will be a Greek b because of the
+# font_region command above).
+ text 12 11 "b1"
+helix 15 10 18
+ text 16 11 "a1"
+helix 22 10 37
+ text 30 11 "a2"
+strand 41 10 45
+ text 43 11 "b2"
+helix 51 10 64
+ text 57 11 "a3"
+helix 67 10 73
+ text 70 11 "a4"
+strand 89 10 91
+ text 90 11 "b3"
+strand 101 10 105
+ text 103 11 "b4"
+helix 115 10 118
+ text 116 11 "a5"
+helix 138 10 163
+ text 151 11 "a6"
+helix 172 10 174
+ text 173 11 "a7"
+#
+#barrel strand 1
+strand 183 10 189
+# set the colour to green for this strand.
+ colour_text_region 183 10 189 10 10
+ text 186 11 "b5"
+#
+helix 197 10 199
+ text 198 11 "a8"
+strand 201 10 202
+ text 201 11 "b6"
+strand 208 10 209
+ text 208 11 "b7"
+#
+#barrel strands 2-6
+strand 210 10 214
+ text 212 11 "b8"
+ colour_text_region 210 10 214 10 10
+strand 220 10 225
+ text 222 11 "b9"
+ colour_text_region 220 10 225 10 10
+strand 231 10 235
+ text 233 11 "b10"
+ colour_text_region 231 10 235 10 10
+strand 238 10 241
+ text 239 11 "b11"
+ colour_text_region 238 10 241 10 10
+strand 249 10 253
+ text 250 11 "b12"
+ colour_text_region 249 10 253 10 10
+#
+helix 255 10 260
+ text 257 11 "a9"
+#
+#
+#barrel strands 7-8
+strand 270 10 273
+ text 271 11 "b13"
+ colour_text_region 270 10 273 10 10
+strand 279 10 286
+ text 282 11 "b14"
+ colour_text_region 279 10 286 10 10
+#
+#
+#
+strand 293 10 294
+ text 293 11 "b15"
+strand 311 10 312
+ text 311 11 "b16"
+helix 313 10 323
+ text 317 11 "a10"
+#
+#Now add arrows for the residues within 8A of the Mn
+#
+# The TEXT command normally just writes out the specified text
+# at the specified position. However, special TEXT objects
+# exist. These all start with the @ symbol.
+#
+colour_text_region 214 12 214 12 1
+#
+# This draws a filled up arrow at position 214,12.
+text 214 12 "@fuparrow"
+colour_text_region 216 12 216 12 1
+text 216 12 "@fuparrow"
+colour_text_region 270 12 270 12 1
+text 270 12 "@fuparrow"
+colour_text_region 330 12 330 12 1
+text 330 12 "@fuparrow"
+#
+text 41 12 "@fuparrow"
+text 252 12 "@fuparrow"
+text 221 12 "@fuparrow"
+text 187 12 "@fuparrow"
+text 285 12 "@fuparrow"
+text 223 12 "@fuparrow"
+text 272 12 "@fuparrow"
+text 218 12 "@fuparrow"
+text 231 12 "@fuparrow"
+#
+#
+# Now set the sequence numbering back to alignment positions.
+relative_to 0
+#
+#
+# From here to the end of the file was created by the alsnum program
+# alsnum creates text commands for numbering a plot relative to a
+# specific sequence, rather than the alignment numbers that are
+# normally shown. Please see the ALSCRIPT manual for further details.
+#
+#
+# Text commands created for numbering
+# Sequence Number: 8
+# Starting Number: 1
+# Interval: 10
+# Position: 9
+TEXT 53 9 "10"
+TEXT 65 9 "20"
+TEXT 78 9 "30"
+TEXT 88 9 "40"
+TEXT 101 9 "50"
+TEXT 111 9 "60"
+TEXT 121 9 "70"
+TEXT 131 9 "80"
+TEXT 141 9 "90"
+TEXT 151 9 "100"
+TEXT 161 9 "110"
+TEXT 171 9 "120"
+TEXT 181 9 "130"
+TEXT 191 9 "140"
+TEXT 201 9 "150"
+TEXT 211 9 "160"
+TEXT 221 9 "170"
+TEXT 231 9 "180"
+TEXT 241 9 "190"
+TEXT 251 9 "200"
+TEXT 261 9 "210"
+TEXT 271 9 "220"
+TEXT 281 9 "230"
+TEXT 297 9 "240"
+TEXT 307 9 "250"
+TEXT 317 9 "260"
+TEXT 327 9 "270"
+TEXT 343 9 "280"
+TEXT 353 9 "290"
+TEXT 363 9 "300"
+TEXT 378 9 "310"
+TEXT 388 9 "320"
+TEXT 398 9 "330"
--- /dev/null
+
+Conversion of CLUSTAL NBRF PIR file to AMPS BLOCKFILE format
+clus2blc: Geoffrey J. Barton (1992)
+
+>P1;3_LYCES 3_LYCES 418 bp PROT 0 JAN 1900, 418 bases, 1CE32436 checksum.
+>P1;U04434 U04434 428 bp PROT 0 JAN 1900, 428 bases, A5C97FB7 checksum.
+>P1;HY6H_HYONI HY6H_HYONI 406 bp PROT 0 JAN 1900, 406 bases, DF93DD1E checksum.
+>P1;S67953 S67953 400 bp PROT 0 JAN 1900, 400 bases, 1276A781 checksum.
+>P1;X73314 X73314 428 bp PROT 0 JAN 1900, 428 bases, 860E176 checksum.
+>P1;CEFE_STRCL CEFE_STRCL 402 bp PROT 0 JAN 1900, 402 bases, 97CFEE75 checksum.
+>P1;EXPA_CEPAC EXPA_CEPAC 422 bp PROT 0 JAN 1900, 422 bases, AE896850 checksum.
+>P1;IPNS_EMENI IPNS_EMENI 403 bp PROT 0 JAN 1900, 403 bases, 4DADFBBB checksum.
+* iteration 1
+ M
+ A
+ L
+ N
+ MG
+ KK
+ TV
+ M AA
+ A QT
+ P GE
+ V VS
+ S SA
+ I AP
+ S TS
+ A LN
+ V TL
+ P MN
+ F EE
+ LAVE
+ PTAM
+ TFRK
+ AVVG
+ ASQE
+ ENAY
+ GWIR
+ ESAP
+ TTSP
+ NKLF
+ VSSG
+ RVKG
+ ASCS
+ SEMD
+ FSDE
+ VFTS
+ RIIK
+ EAPV
+ EPSP
+ DLEE
+ EQYD
+ RKIF
+ PRRI
+ KASW
+ VEES
+ PKNE M
+ HDEK G
+ DVQF S
+ RPPE V
+ FVAA S
+MSGASDTK
+EDNTETSA
+NEDTLTKN
+FVVLLVVV
+PPPHPPPP
+IVIGVTVK
+IVIVLFFI
+NSDVDSRD
+LLLLVLLV
+ P
+ QT
+EEQVIADS
+KGQPDEDP
+LIHVLLLL
+ND IE F
+ G DK G
+ LF D
+ RM D
+ S
+ G
+ D
+GP DK Q
+DR PS A
+ER DYQKA
+RRHEVQSK
+AAHNEGGM
+NELKELKR
+TILMAHVV
+MRVVTQLA
+EGQKRDTQ
+MRQLLEEQ
+IVIIVFLI
+KATADRAD
+DAKDEREA
+AAAAACAA
+CCCSCLVS
+EEQKRRTR
+NDDEQDTD
+WWFWHKKT
+GGGGGGGG
+FILIILIF
+FFFFFFFF
+EQQQFYYY
+LVVLVLLA
+VVIIVTTV
+NDNNNDEN
+HHHHHCSH
+GGGGGGGG
+IVFIVLLI
+PDPPDTV
+HAEDIDD
+EAEEETD
+VLLAMEDN
+MVMIMLHV
+DALAGKTQ
+TDEDRSSR
+VMTLVAAL
+EAMQHKRS
+KREKDDEQ
+MLVVCLTK
+TACGMVCT
+KRKKNIVK
+GDEEEDDE
+HFFFFFFF
+YFFFFFFH
+KAAETEKM
+KLLHMHNS
+CPPVPGGI
+MPAPLSST
+EEEQDEEP
+QDEEVAEE
+RKKEKEEE
+FLEKQKKK
+KRKERRRW
+EFFLAAAD
+LDKIKVVL
+VMPARTTA
+ASKKKSLI
+SGGTVPAR
+KGEPGVDA
+ A Y
+ A N
+ K K
+ F E
+ E H
+ LG Q
+ PS PRD
+ LN TNQ
+ KEDEMAV
+ KQISRRR
+ GKEYRRA
+GGAGGGGG
+LFKYYFFY
+EILGTTSY
+AVYTNGAL
+ SVSSLLS
+ SELFEEI
+ G FSWP
+ E GEEG
+ RSSK
+ TTK
+ AAA
+ FQVV
+VHQQAIVE
+QLLKSTTS
+AQSENNEF
+EGNVLTTC
+VEEEPGGY
+TAEGWSKL
+DVFKKYYN
+LQLKESSP
+DDYGTDDN
+WWWWFYYF
+ERKVSSST
+SEDDLMTP
+TITHRCCD
+FVLLCYYH
+FTAFVSSP
+LYHHAMMR
+RFGKAGGI
+HSCIQTIQ
+LYHWNAGA
+ PPPSDGK
+ VLPSNNT
+PKDSA P
+TAQAA T
+SRDVH H
+NDLND E
+IYVYY V
+SSNRV N
+QRSYLLLV
+VWWWDFFW
+PPPPTPPP
+DDEKLSND
+LKKNGGRE
+DPPPPDGT
+ S K
+ F H
+EAAPS P
+EAKSH G
+YWYYHFFF
+RRRRGEEQ
+EAEEKRDD
+VVVAAIVF
+MVVNYWWA
+REAEQTQE
+DRKEEQDQ
+FYYYCYYY
+ASSGGFFY
+KEVKIDDW
+RQERARRD
+LLVMLQMV
+EMRRNYYF
+KAKEETGG
+LLLVLAAL
+AATVGSAS
+ECMDTRKS
+ERRRKADA
+LLMIIVVL
+LLLFVAAL
+DGDKERRK
+LVYSLEAG
+LLILLVVY
+CSCSGLLA
+EEELLRNL
+NAGGSASA
+LMLLLTVL
+GGGGGGGG
+LLLLITAK
+EDKESEPE
+KTLGRPLE
+GEGHEDAN
+YAYEY F
+LLFMF F
+KADIKGGA
+NRNENGER
+AAEAFVDH
+FCLA F
+YVSG K
+GD IP
+ EDD
+ ADD
+S GFFFT
+K DELVL
+G EDDEA
+PMQINCCS
+NDIVDEDV
+FQQYSPPV
+GKMLILLL
+TVMLLLLI
+KVLKRRRR
+VVTILFLY
+SNNNNRRP
+NFYYYYYY
+YYYYYFFL
+PPPPPPPD
+PRPPTQEP
+CCCCCVVY
+PPPPDPPP
+KQDRKEEE
+PPPPPHDA
+DDSDERRA
+LLSLVSVI
+ AAK
+ T
+ A
+ A
+ EED
+ EEG
+ QET
+ITTAVPPK
+KLLLLLLL
+GGGGGRRS
+LLSVTMMF
+RKGVGAGE
+ARGAPPPW
+HHHHHHHH
+TTYTTYYE
+DDDDDDDD
+APGMPLLV
+GGNSTSSS
+GTLYSMTL
+IIIIVVII
+ITTTTTTT
+LLLIILLV
+LLLLLIVL
+FLQVHQHY
+QQQPQQQQ
+DDDNDTTS
+DL EPPAN
+K CCV
+VVLVVAAQ
+SGPQSNNN
+GGGGGGG
+ FF
+ VV
+ SS
+LLLLLLLL
+QQQQQQQQ
+LAQVVACV
+LTLFCEEE
+KRIKSVVT
+DDVDNGDA
+EGKGDGGA
+ GD
+ RA
+QTTHQAEG
+WWWWWFFY
+IIIYYTVQ
+DTADSDDD
+VVVVILLI
+PQQKPPPE
+PPPYPYTA
+MVIINRLD
+REPPPPPD
+HGTNEDGT
+SAAAAAAG
+IFFLFVMY
+VVVIVLVL
+VVVVIVVI
+NNNHNFFN
+LLLIICCC
+GGGGGGGG
+DDLDDAAS
+QHTQTIVY
+LGLVFAGM
+EHKETTTA
+VLVISLLH
+ILILLVAL
+TSTSTTTT
+NNNNNGGN
+GGEGGGGN
+KRKKIQKY
+YFFYYVVY
+KKEKKKKK
+SNGSGAAA
+VASVCPPP
+LDIYIRKI
+HHHHHHHH
+RQRRRHRR
+VAVTAVVV
+IVVTVAKK
+AVTVVAS
+ PP
+ RG
+ RR
+ DD
+QNDNNQQ
+TSPKSIRW
+DETDMAVV
+GCRKNGGN
+TSDTASSA
+ SSE
+RRRRRRRR
+MLVMKTTQ
+SSSSSSSS
+LIIWLSSL
+AAAPAVVP
+STTVFFFF
+FFLFFFFF
+YQILLLLV
+NNGECRRN
+PPPPPPPL
+GADPSNKG
+SPYSHAPY
+DDSEDDDD
+AACHKFFS
+VTTEVTSV
+IVIVVFFI
+YYEGRSND
+PPPPAVVP
+ALAIPPQF
+KAKPELQD
+T EKEASP
+LVLLLRRR
+VRLLVEEE
+EDNSECWP
+KGQEKGGN
+EEDASFFG
+AANNPDNK
+EPPPPVVS
+EIP SR
+ LL LI
+SDY
+THK
+QPPPR
+VIYKKDPD
+YTSFYGSR
+PFYKPEEE
+KASTDTRP
+FEEKYATL
+VMFKKTTS
+FYAYWFFY
+DRDKPQRG
+DRIDMDED
+YKYYLWWY
+MMLVLILL
+KASYEGGQ
+LRDCMGGN
+YDKKTNNG
+AISLQYYL
+GEDNKVVV
+LLYKRNNS
+KADLYIML
+FRSPRRRI
+QLGQPRRN
+AKV DTDK
+KKK CSKN
+EQP NKPG
+PAY TAAQ
+RKK L AT
+FAI E A
+EDN A E
+AKV F A
+MK K A
+KQ T V
+AQ W P
+MQ V A
+EQ Q A
+SS E A
+DA G P
+PN K V
+IK A S
+AE L T
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--- /dev/null
+#include <stdio.h>
+#include <stdarg.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+#include <errno.h>
+#include <time.h>
+
+#include <gjutil.h>
+#include <gjtimes.h>
+
+#ifndef CLK_TCK
+#include <unistd.h>
+#define CLK_TCK sysconf(_SC_CLK_TCK)
+#endif
+
+
+/* timing routines */
+
+struct tms *t_init = NULL;
+struct tms *t_last = NULL;
+struct tms *t_new = NULL;
+
+clock_t init_times,last_times, new_times;
+
+void GJ_init_times(void)
+{
+
+ extern struct tms *t_init, *t_last, *t_new;
+ extern clock_t init_times, last_times, new_times;
+
+ if(t_init == NULL){
+ t_init = (struct tms *) GJmalloc(sizeof(struct tms));
+ t_new = (struct tms *) GJmalloc(sizeof(struct tms));
+ t_last = (struct tms *) GJmalloc(sizeof(struct tms));
+
+ init_times = times(t_init);
+ new_times = init_times;
+ last_times = init_times;
+
+ copy_tms(t_last,t_init);
+ copy_tms(t_new,t_init);
+ }
+}
+
+void copy_tms(struct tms *l,struct tms *r)
+{
+ l->tms_utime = r->tms_utime;
+ l->tms_stime = r->tms_stime;
+ l->tms_cutime = r->tms_cutime;
+ l->tms_cstime = r->tms_cstime;
+}
+
+
+void GJ_show_times(FILE *fp,int action)
+{
+ /* show the time(s) since init_times was called
+ if action = 0, print the time since the last call to GJ_init_times
+ or GJ_show_times.
+ if action = 1, print as in 0, but add the total time since the
+ first call to GJ_init_times.
+ action = 2, print elapsed and total time +
+ tms_utime, tms_stime, tms_cutime and tms_cstime
+ */
+
+ clock_t init_calling, new_calling, last_calling;
+ clock_t init_child, new_child, last_child;
+
+ extern struct tms *t_init, *t_last, *t_new;
+ extern clock_t init_times, last_times, new_times;
+
+ new_times = times(t_new);
+
+ if(action == 0){
+ fprintf(fp,"Elapsed time: %*6.2f\n",
+ (float)(new_times-last_times)/CLK_TCK);
+ }
+
+ if(action == 1){
+ /* compressed output - shows summary of user + sys for parent and child */
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+
+
+ fprintf(fp,"CPU(P%7.1f%7.1f)(C%7.1f%7.1f)(T%7.1f%7.1f)(E%7.1f%7.1f)\n",
+ (float)(new_calling - last_calling)/CLK_TCK,
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_child - last_child)/CLK_TCK,
+ (float)(new_child - init_child)/CLK_TCK,
+ (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK,
+ (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK,
+ (float)(new_times-last_times)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK);
+ }
+
+
+ if(action == 2){
+ /* full output */
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+
+
+ fprintf(fp," %7s:%7s:%7s|%7s:%7s:%7s:%7s:%7s\n",
+ "user","sys","total","c user","c sys","ctotal","el/tcpu","cum");
+ fprintf(fp,"Master :%7.1f:%7.1f:%7.1f|%7.1f:%7.1f:%7.1f|%7.1f:%7.1f\n",
+ (float)(t_new->tms_utime - t_last->tms_utime)/CLK_TCK,
+ (float)(t_new->tms_stime - t_last->tms_stime)/CLK_TCK,
+ (float)(new_calling - last_calling)/CLK_TCK,
+ (float)(t_new->tms_utime - t_init->tms_utime)/CLK_TCK,
+ (float)(t_new->tms_stime - t_init->tms_stime)/CLK_TCK,
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_times-last_times)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK);
+
+ fprintf(fp,"Children:%7.1f:%7.1f:%7.1f|%7.1f:%7.1f:%7.1f|%7.1f:%7.1f\n",
+ (float)(t_new->tms_cutime - t_last->tms_cutime)/CLK_TCK,
+ (float)(t_new->tms_cstime - t_last->tms_cstime)/CLK_TCK,
+ (float)(new_child - last_child)/CLK_TCK,
+ (float)(t_new->tms_cutime - t_init->tms_cutime)/CLK_TCK,
+ (float)(t_new->tms_cstime - t_init->tms_cstime)/CLK_TCK,
+ (float)(new_child - init_child)/CLK_TCK,
+ (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK,
+ (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK);
+ }
+ if(action == 3){
+ /* very compressed output - shows cumparent, cumchild + sum of the two and elapsed time summary*/
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+ fprintf(fp,"Cum CPU Time: Parent: %*7.1f Child: %*7.1f Total: %*7.1f Elapsed: %7.1f\n",
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_child - init_child)/CLK_TCK,
+ (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK);
+ }
+
+ copy_tms(t_last,t_new);
+ last_times = new_times;
+}
+
+
+char *GJ_get_times(int action)
+{
+ /* show the time(s) since init_times was called
+ if action = 0, print the time since the last call to GJ_init_times
+ or GJ_show_times.
+ if action = 1, print as in 0, but add the total time since the
+ first call to GJ_init_times.
+
+ */
+
+ clock_t init_calling, new_calling, last_calling;
+ clock_t init_child, new_child, last_child;
+
+ char *outbuf;
+
+ extern struct tms *t_init, *t_last, *t_new;
+ extern clock_t init_times, last_times, new_times;
+
+ new_times = times(t_new);
+
+ outbuf = (char *) GJmalloc(sizeof(char) *200);
+
+ if(action == 0){
+ sprintf(outbuf,"%7.1f\0",
+ (float)(new_times-last_times)/CLK_TCK);
+ }
+#ifdef KEEP_THIS
+ if(action == 1){
+ /* compressed output - shows summary of user + sys for parent and child */
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+ sprintf(outbuf,"%7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f\0",
+ (float)(new_calling - last_calling)/CLK_TCK,
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_child - last_child)/CLK_TCK,
+ (float)(new_child - init_child)/CLK_TCK,
+ (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK,
+ (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK,
+ (float)(new_times-last_times)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK);
+ }
+#else
+ /* this is a version that ignores the child processes since these
+ appear not to be reported on the SGI */
+ if(action == 1){
+ /* compressed output - shows summary of user + sys for parent and child */
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+ sprintf(outbuf,"%7.1f %7.1f %7.1f %7.1f\0",
+ (float)(new_calling - last_calling)/CLK_TCK,
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_times-last_times)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK);
+ }
+#endif
+
+ if(action == 2){
+ /* full output */
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+
+
+ sprintf(outbuf,
+ "%7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f",
+ (float)(t_new->tms_utime - t_last->tms_utime)/CLK_TCK,
+ (float)(t_new->tms_stime - t_last->tms_stime)/CLK_TCK,
+ (float)(new_calling - last_calling)/CLK_TCK,
+ (float)(t_new->tms_utime - t_init->tms_utime)/CLK_TCK,
+ (float)(t_new->tms_stime - t_init->tms_stime)/CLK_TCK,
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_times-last_times)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK,
+ (float)(t_new->tms_cutime - t_last->tms_cutime)/CLK_TCK,
+ (float)(t_new->tms_cstime - t_last->tms_cstime)/CLK_TCK,
+ (float)(new_child - last_child)/CLK_TCK,
+ (float)(t_new->tms_cutime - t_init->tms_cutime)/CLK_TCK,
+ (float)(t_new->tms_cstime - t_init->tms_cstime)/CLK_TCK,
+ (float)(new_child - init_child)/CLK_TCK,
+ (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK,
+ (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK);
+ }
+
+ if(action == 3){
+ /* very compressed output - shows cumparent, cumchild + sum of the two and elapsed time summary*/
+ init_calling = t_init->tms_utime + t_init->tms_stime;
+ init_child = t_init->tms_cutime + t_init->tms_cstime;
+
+ last_calling = t_last->tms_utime + t_last->tms_stime;
+ new_calling = t_new->tms_utime + t_new->tms_stime;
+
+ last_child = t_last->tms_cutime + t_last->tms_cstime;
+ new_child = t_new->tms_cutime + t_new->tms_cstime;
+
+ sprintf(outbuf,"%7.1f %7.1f %7.1f %7.1f\0",
+ (float)(new_calling - init_calling)/CLK_TCK,
+ (float)(new_child - init_child)/CLK_TCK,
+ (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK,
+ (float)(new_times-init_times)/CLK_TCK);
+ }
+
+ copy_tms(t_last,t_new);
+ last_times = new_times;
+ outbuf = (char *) GJrealloc(outbuf,sizeof(char) * (strlen(outbuf) + 1));
+ return outbuf;
+}
+
+void GJ_reset_times()
+{
+ extern struct tms *t_init, *t_new, *t_last;
+
+ if(t_init != NULL){
+ GJfree(t_init);
+ t_init = NULL;
+ }
+ if(t_new != NULL){
+ GJfree(t_new);
+ t_new = NULL;
+ }
+ if(t_last != NULL){
+ GJfree(t_last);
+ t_last = NULL;
+ }
+}
+#define DELETE
+#ifndef DELETE
+main(int argc,char **argv)
+{
+ int i,j;
+ float l;
+ GJ_init_times();
+ /* fprintf(stderr,"(0)%s\n",GJ_get_times(0));*/
+ /* fprintf(stderr,"(1)%s\n",GJ_get_times(1));*/
+ /* fprintf(stderr,"(2)%s\n",GJ_get_times(2));*/
+ fprintf(stderr,"(3)%s\n",GJ_get_times(3));
+
+ for(i=0;i<5000;++i){
+ for(j=0;j<5000;++j){
+ l = i*j;
+ }
+ }
+ /* fprintf(stderr,"(0)%s\n",GJ_get_times(0));*/
+ /* fprintf(stderr,"(1)%s\n",GJ_get_times(1));*/
+ /* fprintf(stderr,"(2)%s\n",GJ_get_times(2));*/
+ fprintf(stderr,"(3)%s\n",GJ_get_times(3));
+ for(i=0;i<10000;++i){
+ for(j=0;j<5000;++j){
+ l = i*j;
+ }
+ }
+ /* fprintf(stderr,"(0)%s\n",GJ_get_times(0));*/
+ /* fprintf(stderr,"(1)%s\n",GJ_get_times(1));*/
+ /* fprintf(stderr,"(2)%s\n",GJ_get_times(2));*/
+ fprintf(stderr,"(3)%s\n",GJ_get_times(3));
+ GJ_reset_times();
+}
+#endif
+
+
+
+
+
--- /dev/null
+#ifndef GJ_TIMES_H
+#define GJ_TIMES_H
+
+#include <sys/types.h>
+#include <sys/times.h>
+#include <limits.h>
+
+void GJ_init_times(void);
+void GJ_show_times(FILE *fp,int action);
+void GJ_reset_times(void);
+char *GJ_get_times(int action);
+
+void copy_tms(struct tms *l,struct tms *r);
+
+#endif /*GJ_TIMES */
+
--- /dev/null
+
+/****************************************************************************
+
+gjutil.c: Various utility routines - error checking malloc and
+free, string functions etc...
+
+Copyright: Geoffrey J. Barton (1992, 1993, 1995, 1997)
+email: geoff@ebi.ac.uk
+
+This software is made available for educational and non-commercial research
+purposes.
+
+For commercial use, a commercial licence is required - contact the author
+at the above address for details.
+
+
+******************************************************************************/
+#include <stdio.h>
+#include <stdarg.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+#include <errno.h>
+#include <time.h>
+
+#include <gjtimes.h>
+#include <gjutil.h>
+
+/* define pointers for standard streams to allow redefinition to files */
+
+FILE *std_err;
+FILE *std_in;
+FILE *std_out;
+
+/* clock externs */
+clock_t start_time, end_time,initial_time,final_time;
+
+void *GJmalloc(size_t size)
+/* malloc with simple error check */
+/* G. J. Barton 1992 */
+{
+ void *ptr;
+ ptr = (void *) malloc(size);
+ if(ptr == NULL){
+ GJerror("malloc error");
+ exit(0);
+ }
+ return ptr;
+}
+
+void *GJrealloc(void *ptr,size_t size)
+/* realloc with error check */
+/* G. J. Barton 1992 */
+{
+ ptr = (void *) realloc(ptr,size);
+ if(ptr == NULL){
+ GJerror("realloc error");
+ exit(0);
+ }
+ return ptr;
+}
+void *GJmallocNQ(size_t size)
+/* as for GJmalloc, but returns NULL on error*/
+/* G. J. Barton 1992 */
+{
+ void *ptr;
+ ptr = (void *) malloc(size);
+ if(ptr == NULL){
+ GJerror("malloc error");
+ return NULL;
+ }
+ return ptr;
+}
+
+void *GJreallocNQ(void *ptr,size_t size)
+/* as for GJrealloc with error check but returns NULL on error*/
+/* G. J. Barton 1992 */
+{
+ ptr = (void *) realloc(ptr,size);
+ if(ptr == NULL){
+ GJerror("realloc error");
+ return NULL;
+ }
+ return ptr;
+}
+void GJfree(void *ptr)
+/* free with error check */
+/* G. J. Barton 1992 */
+{
+ if(ptr == NULL){
+ GJerror("Attempt to free NULL pointer");
+ exit(0);
+ }
+ free(ptr);
+}
+
+void GJerror(const char *prefix)
+/* writes error message contained in prefix and contents of errno
+ to std_err.
+*/
+/* G. J. Barton 1992 */
+{
+ if(prefix != NULL){
+ if(*prefix != '\0'){
+ fprintf(std_err,"%s: ",prefix);
+ }
+ }
+ fprintf(std_err,"%s\n",strerror(errno));
+}
+
+/*
+error: calls GJerror
+*/
+void error(const char *str,int flag)
+{
+ GJerror(str);
+ if(flag)exit(0);
+}
+
+
+char *GJstoupper(const char *s)
+/* return a copy of s in upper case */
+/* G. J. Barton 1992 */
+{
+ char *temp;
+ int i;
+ temp = GJstrdup(s);
+ i=0;
+ while(temp[i] != '\0'){
+ temp[i] = toupper(temp[i]);
+ ++i;
+ }
+ return temp;
+}
+char *GJstolower(const char *s)
+/* return a copy of s in lower case */
+/* G. J. Barton 1992 */
+{
+ char *temp;
+ int i;
+ temp = GJstrdup(s);
+ i=0;
+ while(temp[i] != '\0'){
+ temp[i] = tolower(temp[i]);
+ ++i;
+ }
+ return temp;
+}
+char *GJstoup(char *s)
+/* return s in upper case */
+/* G. J. Barton 1992 */
+{
+ int i;
+ i=0;
+ while(s[i] != '\0'){
+ s[i] = toupper(s[i]);
+ ++i;
+ }
+ return s;
+}
+char *GJstolo(char *s)
+/* return s in lower case */
+/* G. J. Barton 1992 */
+{
+ int i;
+ i=0;
+ while(s[i] != '\0'){
+ s[i] = tolower(s[i]);
+ ++i;
+ }
+ return s;
+}
+
+char *GJstrdup(const char *s)
+/* returns a pointer to a copy of string s */
+/* G. J. Barton 1992 */
+
+{
+ char *temp;
+ temp = (char *) GJmalloc(sizeof(char) * (strlen(s)+1));
+ temp = strcpy(temp,s);
+ return temp;
+}
+
+char *GJstrrename(char *old,const char *new)
+/* takes old which is a pointer to a string, then replaces the contents
+ of the string with new, reallocating to the correct size
+*/
+{
+ int nlen;
+ nlen = strlen(new);
+ old = (char *) GJrealloc(old,sizeof(char) * (nlen + 1));
+ old = strcpy(old,new);
+ return old;
+}
+
+
+
+FILE *GJfopen(const char *fname,const char *type,int action)
+/* a file open function with error checking. The third argument
+is set to 0 if we want a failed open to return, or 1 if we
+want a failed open to exit the program.
+*/
+/* G. J. Barton 1992 */
+/* modified July 1995 - error message only printed if action is 1 */
+{
+ FILE *ret_val;
+ ret_val = fopen(fname,type);
+ if(ret_val == NULL){
+ /* GJerror(strcat("Cannot Open File: ",fname));*/
+ if(action == 1){
+ GJerror(strcat("Cannot Open File: ",fname));
+ exit(1);
+ }
+ }
+ return ret_val;
+}
+
+int GJfclose(FILE *fp,int action)
+/* a file close function with error checking. The second argument
+is set to 0 if we want a failed close to return, or 1 if we
+want a failed close to exit the program.
+*/
+/* G. J. Barton 1992 */
+{
+ int ret_val;
+ ret_val = fclose(fp);
+ if(ret_val != 0){
+ if(action == 1){
+ GJerror("Error closing File");
+ exit(1);
+ }
+ }
+ return ret_val;
+}
+
+
+GJFILE *GJfilemake(const char *name,const char *type,int action)
+/* If action = 1 then
+Tries to open the file with the given name. If successful returns
+a pointer to a struct file structure with the name and filehandle. If
+the open fails, or action= 0 then returns a struct file structure
+with the name and a NULL filehandle */
+/* G. J. Barton 1995 */
+{
+ GJFILE *ret_val;
+ ret_val = (GJFILE *) GJmalloc(sizeof(GJFILE));
+ ret_val->name = GJstrdup(name);
+ if(action == 1) {
+ ret_val->handle = GJfopen(ret_val->name,type,0);
+ }else if(action == 0){
+ ret_val->handle = NULL;
+ }
+ return ret_val;
+}
+
+GJFILE *GJfilerename(GJFILE *ret_val, const char *name)
+/* When passed the fval structure - renames the name part of the
+file structure to name, if the handle is non null it tries to close
+the file, then sets the file handle to NULL. */
+/* G. J. Barton 1995 */
+{
+ if(ret_val->name != NULL) {
+ GJfree(ret_val->name);
+ ret_val->name = GJstrdup(name);
+ }
+ if(ret_val->handle != NULL) {
+ GJfclose(ret_val->handle,0);
+ ret_val->handle = NULL;
+ }
+ return ret_val;
+}
+
+GJFILE *GJfileclose(GJFILE *ret_val,int action)
+/* Closes a file named in the struct file structure returns the struct
+ file structure */
+
+/* G. J. Barton July 1995 */
+{
+ STD_FILES;
+
+ if(GJfclose(ret_val->handle,0) == 0){
+ return ret_val;
+ }else {
+ if(action == 1){
+ GJerror("Error closing File");
+ fprintf(std_err,"%s\n",ret_val->name);
+ exit(-1);
+ }
+ }
+ return ret_val;
+}
+
+GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action)
+/* Opens a file named in the struct file structure */
+
+/* G. J. Barton October 1995 */
+{
+ STD_FILES;
+
+ ret_val->handle = GJfopen(ret_val->name,type,0);
+ if(ret_val->handle == NULL){
+ if(action == 1){
+ GJerror("Error opening File");
+ fprintf(std_err,"%s\n",ret_val->name);
+ exit(-1);
+ }
+ }
+ return ret_val;
+}
+
+GJFILE *GJfileclean(GJFILE *ret_val,int action)
+/* Closes the file then sets the file pointer to NULL, then
+ frees the filename string */
+
+/* G. J. Barton July 1995 */
+{
+ if(GJfclose(ret_val->handle,0) == 0) {
+ ret_val->handle = NULL;
+ GJfree(ret_val->name);
+ return ret_val;
+ }else {
+ if(action == 1){
+ GJerror("Error closing File");
+ fprintf(std_err,"%s\n",ret_val->name);
+ exit(-1);
+ }
+ }
+ return ret_val;
+}
+
+void GJinitfile(void)
+/* just set the standard streams */
+{
+ std_err = stderr;
+ std_in = stdin;
+ std_out = stdout;
+}
+
+char *GJfnonnull(char *string)
+/* return pointer to first non null character in the string */
+/* this could cause problems if the string is not null terminated */
+{
+ while(*string != '\0'){
+ ++string;
+ }
+ return ++string;
+}
+
+char *GJstrappend(char *string1, char *string2)
+/* appends string2 to the end of string2. Any newline characters are removed
+from string1, then the first character of string2 overwrites the null at the
+end of string1.
+string1 and string2 must have been allocated with malloc.
+*/
+/* G. J. Barton July 1992 */
+{
+ char *ret_val;
+ ret_val = GJremovechar(string1,'\n');
+ ret_val = (char *) GJrealloc(ret_val,
+ sizeof(char) * (strlen(ret_val) + strlen(string2) + 1));
+ ret_val = strcat(ret_val,string2);
+ return ret_val;
+}
+
+char *GJremovechar(char *string,char c)
+/* removes all instances of character c from string
+ returns a pointer to the reduced, null terminated string
+ 11/8/1996: couple of bugs found in this routine.
+ the length of the string returned was too short by 2 bytes.
+ This is a dodgy routine since string is freed.
+*/
+/* G. J. Barton (July 1992) */
+{
+ char *temp;
+ int j,i,nchar;
+ nchar = 0;
+ i=0;
+ while(string[i] != '\0'){
+ if(string[i] == c){
+ ++nchar;
+ }
+ ++i;
+ }
+ if(nchar == 0){
+ return string;
+ }else{
+ temp = (char *) GJmalloc(sizeof(char) * (strlen(string)-nchar + 1));
+ j=0;
+ i=0;
+ while(string[i] != '\0'){
+ if(string[i] != c){
+ temp[j] = string[i];
+ ++j;
+ }
+ ++i;
+ }
+ temp[j] = '\0';
+ GJfree(string);
+ return temp;
+ }
+}
+
+char *GJremovechar2(char *string,char c)
+/* removes all instances of character c from string
+ returns a pointer to the reduced, null terminated string
+*/
+/* G. J. Barton (July 1992) */
+{
+ char *temp;
+ int i,k,len;
+ k=0;
+ len=strlen(string);
+ temp = (char *) GJmalloc(sizeof(char) * (len+1));
+ for(i=0;i<(len+1);++i){
+ if(string[i] != c){
+ temp[k] = string[i];
+ ++k;
+ }
+ }
+ for(i=0;i<(strlen(temp)+1);++i){
+ string[i] = temp[i];
+ }
+ GJfree(temp);
+ return string;
+}
+
+
+char *GJsubchar(char *string,char c2,char c1)
+/* substitutes c1 for c2 in string
+*/
+/* G. J. Barton (July 1992) */
+{
+ int i;
+
+ i=0;
+ while(string[i] != '\0'){
+ if(string[i] == c1){
+ string[i] = c2;
+ }
+ ++i;
+ }
+ return string;
+}
+
+/* create a string and if fchar != NULL fill with characters */
+/* always set the len-1 character to '\0' */
+
+char *GJstrcreate(size_t len,char *fchar)
+{
+ char *ret_val;
+ ret_val = (char *) GJmalloc(sizeof(char) * len);
+ --len;
+ ret_val[len] = '\0';
+ if(fchar != NULL){
+ while(len > -1){
+ ret_val[len] = *fchar;
+ --len;
+ }
+ }
+ return ret_val;
+}
+
+/* searches for string s2 in string s1 and returns pointer to first instance
+of s2 in s1 or NULL if no instance found. s1 and s2 must be null terminated
+*/
+char *GJstrlocate(char *s1, char *s2)
+{
+ int i=0;
+ int j=0;
+ int k;
+ if(strlen(s1) == 0 || strlen(s2) == 0) return NULL;
+ while(i < strlen(s1)){
+ j=0;
+ k=i;
+ while(j < strlen(s2) && s1[k] == s2[j]){
+ ++k;
+ ++j;
+ }
+ if(j == strlen(s2)) return &s1[i];
+ ++i;
+ }
+ return NULL;
+}
+#include <stdlib.h>
+#include <string.h>
+
+
+/* GJstrtok()
+
+This version of strtok places the work pointer at the location of the first
+character in the next token, rather than just after the last character of the
+current token. This is useful for extracting quoted strings
+*/
+
+char *GJstrtok(char *input_string,const char *token_list)
+{
+ static char *work;
+ char *return_ptr;
+
+ if(input_string != NULL){
+ /* first call */
+ work = input_string;
+ }
+
+ /* search for next non-token character */
+ while(strchr(token_list,*work)!=NULL){
+ ++work;
+ }
+
+ if(*work == '\0'){
+ /* if we've reached the end of string, then return NULL */
+ return NULL;
+ }else{
+ return_ptr = (char *) work;
+ while(strchr(token_list,*work) == NULL){
+ if(*work == '\0'){
+ /* end of the string */
+ return return_ptr;
+ }else{
+ ++work;
+ }
+ }
+ *work = '\0';
+ ++work;
+ /* now increment work until we find the next non-delimiter character */
+ while(strchr(token_list,*work) != NULL){
+ if(*work == '\0'){
+ break;
+ }else{
+ ++work;
+ }
+ }
+ return return_ptr;
+ }
+}
+/**************************************************************************
+return a pointer to space for a rectangular unsigned character array
+Version 2.0 ANSI and uses GJmallocNQ
+--------------------------------------------------------------------------*/
+
+unsigned char **uchararr(int i,int j)
+{
+ unsigned char **temp;
+ int k, rowsiz;
+
+ temp = (unsigned char **) GJmallocNQ(sizeof(unsigned char *) * i);
+ if(temp == NULL) return NULL;
+
+ rowsiz = sizeof(unsigned char) * j;
+
+ for (k = 0; k < i; ++k){
+ temp[k] = (unsigned char *) GJmallocNQ(rowsiz);
+ if(temp[k] == NULL) return NULL;
+ }
+ return temp;
+}
+
+/**************************************************************************
+free up space pointed to by rectangular unsigned character array
+-------------------------------------------------------------------------*/
+void ucharfree(unsigned char **array,int i)
+
+{
+ int k;
+
+ for (k = 0; k < i; ++k){
+ GJfree((char *) array[k]);
+ }
+ GJfree((char *) array);
+
+}
+
+/**************************************************************************
+return a pointer to space for a rectangular signed character array
+Version 2.0 ANSI
+--------------------------------------------------------------------------*/
+signed char **chararr(int i,int j)
+
+{
+ signed char **temp;
+ int k, rowsiz;
+
+ temp = (signed char **) GJmallocNQ(sizeof(char *) * i);
+
+ if(temp == NULL) return NULL;
+
+ rowsiz = sizeof(char) * j;
+
+ for (k = 0; k < i; ++k){
+ temp[k] = (signed char *) GJmallocNQ(rowsiz);
+ if(temp[k] == NULL) return NULL;
+ }
+ return temp;
+}
+
+
+/* mcheck - check a call to malloc - if the call has failed, print the
+error message and exit the program */
+/* ANSI Version - also uses GJerror routine and ptr is declared void*/
+
+void mcheck(void *ptr,char *msg)
+
+{
+ if(ptr == NULL){
+ GJerror("malloc/realloc error");
+ exit(0);
+ }
+}
+
+/* set a string to blanks and add terminating nul */
+char *GJstrblank(char *string,int len)
+
+{
+ --len;
+ string[len] = '\0';
+ --len;
+ while(len > -1){
+ string[len] = ' ';
+ --len;
+ }
+ return string;
+}
+
+/* Initialise an unsigned char array */
+void GJUCinit(unsigned char **array,int i,int j,unsigned char val)
+{
+ int k,l;
+
+ for(k=0;k<i;++k){
+ for(l=0;l<j;++l){
+ array[k][l] = val;
+ }
+ }
+}
+/*Initialise a signed char array */
+
+void GJCinit(signed char **array,int i,int j,char val)
+
+{
+ int k,l;
+
+ for(k=0;k<i;++k){
+ for(l=0;l<j;++l){
+ array[k][l] = val;
+ }
+ }
+}
+
+/* Initialise an integer vector */
+void GJIinit(int *array,int i,int val)
+{
+ int k;
+
+ for(k=0;k<i;++k){
+ array[k] = val;
+ }
+}
+
+/******************************************************************
+GJcat: concatenate N NULL terminated strings into a single string.
+The source strings are not altered
+Author: G. J. Barton (Feb 1993)
+------------------------------------------------------------------*/
+char *GJcat(int N,...)
+{
+ va_list parminfo;
+ int i,j,k;
+ char **values; /*input strings */
+ int *value_len; /*lengths of input strings */
+ int ret_len; /*length of returned string */
+ char *ret_val; /*returned string */
+
+ ret_len = 0;
+ values = (char **) GJmalloc(sizeof(char *) * N);
+ value_len = (int *) GJmalloc(sizeof(int *) * N);
+
+ va_start(parminfo,N);
+
+ /* Get pointers and lengths for the N arguments */
+ for(i=0;i<N;++i){
+ values[i] = va_arg(parminfo,char *);
+ value_len[i] = strlen(values[i]);
+ ret_len += value_len[i];
+ }
+
+ ret_val = (char *) GJmalloc(sizeof(char) * (ret_len+1));
+
+ /* Transfer input strings to output string */
+ k=0;
+ for(i=0;i<N;++i){
+ for(j=0;j<value_len[i];++j){
+ ret_val[k] = values[i][j];
+ ++k;
+ }
+ }
+ ret_val[k] = '\0';
+ GJfree(values);
+ GJfree(value_len);
+
+ va_end(parminfo);
+
+ return ret_val;
+}
+
+/************************************************************************
+
+GJGetToken:
+
+The aim of this routine is to emulate the strtok() function, but reading
+from a file. The functionality may differ slightly...
+
+Characters are read from the file until a character that is found in
+delim is encountered. When this occurs, token is returned. If the
+file consists entirely of delimiters, then token is freed
+and NULL is returned. Similarly, if end of file is encountered.
+
+------------------------------------------------------------------------*/
+
+
+char *GJGetToken(FILE *in, const char *delim)
+
+{
+ int i;
+ int c;
+ char *token;
+
+ i=0;
+
+ token = GJmalloc(sizeof(char));
+
+ while((c=fgetc(in)) != EOF){
+ if(strchr(delim,c) == NULL){
+ /* not a delimiter */
+ token[i++] = c;
+ token = GJrealloc(token,sizeof(char) * (i+1));
+ }else if(i>0){
+ token[i] = '\0';
+ return token;
+ }
+ }
+/* GJerror("End of File Encountered");*/
+ GJfree(token);
+ return NULL;
+}
+
+struct tokens *GJgettokens(const char *delims, char *buff)
+/* This splits a buffer into tokens at each position defined by delims.*/
+/* The structure returned contains the number of tokens and the */
+/* tokens themselves as a char ** array */
+{
+ char *token;
+ struct tokens *tok;
+
+ token = strtok(buff,delims);
+ if(token == NULL) return NULL;
+
+ tok = (struct tokens *) GJmalloc(sizeof(struct tokens));
+ tok->ntok = 0;
+ tok->tok = (char **) GJmalloc(sizeof(char *));
+ tok->tok[0] = GJstrdup(token);
+ ++tok->ntok;
+ while((token = strtok(NULL,delims)) != NULL) {
+ tok->tok = (char **) GJrealloc(tok->tok,sizeof(char *) * (tok->ntok+1));
+ tok->tok[tok->ntok] = GJstrdup(token);
+ ++tok->ntok;
+ }
+
+ return tok;
+}
+
+void GJfreetokens(struct tokens *tok)
+/* frees a tokens structure */
+
+{
+ int i;
+ for(i=0;i<tok->ntok;++i) {
+ GJfree(tok->tok[i]);
+ }
+ GJfree(tok->tok);
+ GJfree(tok);
+ tok = NULL; /* add this to avoid odd pointer 27/6/1997*/
+}
+
+char * GJtoktostr(struct tokens *tok,char delim,int s, int e)
+
+/*
+ returns a string with the tokens between s and e inclusive written to
+ it separated by delim
+ the tok structure is unmodified.
+*/
+
+{
+ int n, i, j,k;
+ char *ret_val;
+
+ n = 0;
+
+
+ if(s < 0 || s >= tok->ntok) s = 0;
+ if(e < 0 || e >= tok->ntok) e = tok->ntok - 1;
+
+ for(i=s;i<=e;++i){
+ n += strlen(tok->tok[i]);
+ ++n;
+ }
+
+ ret_val = (char *) GJmalloc(sizeof(char) * n);
+ j = 0;
+ for(i=s;i<=e;++i){
+ for(k=0;k<strlen(tok->tok[i]);++k){
+ ret_val[j] = tok->tok[i][k];
+ ++j;
+ }
+ ret_val[j++] = delim;
+ }
+ ret_val[n-1] = '\0';
+ return ret_val;
+}
+
+/*
+void GJ_start_clock()
+{
+ extern clock_t start_time, end_time,initial_time,final_time;
+ start_time = clock();
+ initial_time = start_time;
+}
+
+void GJ_stop_clock(FILE *fp)
+{
+ extern clock_t start_time, end_time,initial_time,final_time;
+ end_time = clock();
+ fprintf(fp,"CPU time: %f seconds\n",
+ ((float) (end_time - start_time))/CLOCKS_PER_SEC);
+}
+*/
+
+
+void GJ_start_clock(void)
+{
+ GJ_init_times();
+}
+
+void GJ_stop_clock(FILE *fp)
+{
+ GJ_show_times(fp,3);
+ GJ_reset_times();
+}
+
+
+void GJindexx(int *arrin,int n,int *indx)
+ /* indexed heap sort - adapted from the NR routine indexx.
+ inarr is an integer array to sort,
+ indx is the returned index array
+ */
+{
+ int l,j,ir,indxt,i;
+ float q;
+
+ for (j=1;j<=n;j++) indx[j]=j;
+ l=(n >> 1) + 1;
+ ir=n;
+ for (;;) {
+ if (l > 1)
+ q=arrin[(indxt=indx[--l])];
+ else {
+ q=arrin[(indxt=indx[ir])];
+ indx[ir]=indx[1];
+ if (--ir == 1) {
+ indx[1]=indxt;
+ return;
+ }
+ }
+ i=l;
+ j=l << 1;
+ while (j <= ir) {
+ if (j < ir && arrin[indx[j]] < arrin[indx[j+1]]) j++;
+ if (q < arrin[indx[j]]) {
+ indx[i]=indx[j];
+ j += (i=j);
+ }
+ else j=ir+1;
+ }
+ indx[i]=indxt;
+ }
+}
+
+
+
+void GJpline(FILE *fp,char c,int n)
+/* print n copies of c to fp terminated by newline */
+{
+ int i;
+ for(i=0;i<n;++i){
+ fprintf(fp,"%c",c);
+ }
+ fprintf(fp,"\n");
+}
+
+char *GJlocaltime(void)
+{
+ time_t t;
+ char *ret_val;
+ t = time(NULL);
+ ret_val=asctime(localtime(&t));
+ return ret_val;
+}
+
+void GJpstring(FILE *fp,char *s,int n)
+/* print a string so that it occupies n characters */
+/* strings are left-justified and either truncated or padded on output */
+{
+ int i,l;
+
+
+ l = strlen(s);
+
+ if(l > n){
+ for(i=0;i<n;++i){
+ fprintf(fp,"%c",s[i]);
+ }
+ }else{
+ for(i=0;i<l;++i){
+ fprintf(fp,"%c",s[i]);
+ }
+ for(i=l;i<n;++i){
+ fprintf(fp," ");
+ }
+ }
+}
+
+/* return min and max of an integer vector */
+IRANGE *irange(int *ivec,int n)
+{
+ int i;
+ int imin,imax;
+ IRANGE *ret_val;
+
+ imin = imax = ivec[0];
+
+ for(i=1;i<n;++i){
+ if(ivec[i] > imax) imax = ivec[i];
+ if(ivec[i] < imin) imin = ivec[i];
+ }
+
+ ret_val = (IRANGE *) GJmalloc(sizeof(IRANGE));
+ ret_val->min = imin;
+ ret_val->max = imax;
+
+ return ret_val;
+}
+
+/*
+
+$Id: gjutil.c,v 1.1.1.1 1999/07/09 13:34:10 geoff Exp $
+
+$Log: gjutil.c,v $
+Revision 1.1.1.1 1999/07/09 13:34:10 geoff
+Import alscript 2_07 with the cygwin executables.
+
+Revision 1.9 1999/07/09 13:34:10 geoff
+modified these as a test
+
+Revision 1.8 1998/08/11 15:38:50 geoff
+Modified the copyright notice to reflect the new
+ownership of this software.
+
+Revision 1.7 1997/06/29 00:43:57 gjb
+Changes to add sysinfo calls and test of license reading routines
+
+Revision 1.6 1997/06/27 16:42:41 gjb
+Add trlic.c test.lic and clean up gjutil.c
+
+Revision 1.5 1997/06/27 07:17:31 gjb
+Added rlic.c linfo.h and
+changes to gjutil.c to give better support for
+token manipulation
+
+Revision 1.4 1997/05/12 11:10:53 gjb
+Re-added gjutil.c and gjutil.h to repository
+after deleting them
+
+Revision 1.2 1997/05/12 10:47:52 gjb
+Modified CVS header and log position
+
+*/
--- /dev/null
+#ifndef GJ_UTIL_H
+#define GJ_UTIL_H
+
+/*
+$Id: gjutil.h,v 1.1.1.1 1997/06/27 07:17:32 geoff Exp $
+*/
+
+#include <stdio.h>
+
+
+typedef int GJ_LIM; /* type for limits - e.g. max buffer length */
+typedef int GJ_PEN; /* type for gap penalties */
+typedef int GJ_FLG; /* Flag type - usually just 1 or 0 */
+typedef float GJ_FLOAT; /* Single precision floating point type */
+typedef double GJ_DBL; /* a double precision floating point type */
+typedef int GJ_S_COUNT; /* small counter */
+typedef long GJ_L_COUNT; /* long counter */
+
+typedef struct { /* structure to hold a filename and */
+ char *name; /* associated handle */
+ FILE *handle;
+} GJFILE;
+
+struct tokens { /* structure to hold tokens parsed from */
+ int ntok; /* string with strtok */
+ char **tok;
+};
+
+typedef struct {
+ int min;
+ int max;
+} IRANGE;
+
+#define STD_FILES extern FILE *std_in,*std_out,*std_err
+
+/* utility.h function definitions */
+
+void *GJmalloc(size_t);
+void *GJrealloc(void *,size_t);
+void *GJmallocNQ(size_t);
+void *GJreallocNQ(void *,size_t);
+void GJfree(void *);
+void GJerror(const char *);
+char *GJstrdup(const char *);
+char *GJstoupper(const char *);
+char *GJstolower(const char *);
+char *GJstoup(char *);
+char *GJstolo(char *);
+
+FILE *GJfopen(const char *, const char *,int);
+int GJfclose(FILE *,int);
+GJFILE *GJfilemake(const char *name,const char *type,int action);
+GJFILE *GJfilerename(GJFILE *ret_val, const char *name);
+GJFILE *GJfileclose(GJFILE *ret_val,int action);
+GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action);
+GJFILE *GJfileclean(GJFILE *fval,int action);
+void GJinitfile(void);
+
+char *GJfnonnull(char *);
+char *GJstrappend(char *,char *);
+char *GJremovechar(char *,char);
+char *GJremovechar2(char *string,char c);
+char *GJstrcreate(size_t, char *);
+char *GJstrlocate(char *,char *);
+char *GJsubchar(char *,char,char);
+char *GJstrtok(char *,const char *);
+void error(const char *, int);
+unsigned char **uchararr(int,int);
+void ucharfree(unsigned char **array,int i);
+signed char **chararr(int,int);
+void GJCinit(signed char **,int ,int ,char );
+void mcheck(void *, char *);
+char *GJstrblank(char *, int);
+void GJUCinit(unsigned char **,int ,int ,unsigned char );
+char *GJcat(int N,...);
+struct tokens *GJgettokens(const char *delims, char *buff);
+void GJfreetokens(struct tokens *tok);
+char * GJtoktostr(struct tokens *tok, char delim, int s, int e);
+void GJ_start_clock(void);
+void GJ_stop_clock(FILE *fp);
+char *GJstrrename(char *old,const char *new);
+void GJindexx(int *arrin,int n,int *indx);
+
+void GJpline(FILE *fp,char c,int n);
+char *GJlocaltime(void);
+void GJpstring(FILE *fp,char *s,int n);
+
+IRANGE *irange(int *ivec, int n);
+
+/*
+$Log: gjutil.h,v $
+Revision 1.1.1.1 1997/06/27 07:17:32 geoff
+Import alscript 2_07 with the cygwin executables.
+
+Revision 1.5 1997/06/27 07:17:32 gjb
+Added rlic.c linfo.h and
+changes to gjutil.c to give better support for
+token manipulation
+
+Revision 1.4 1997/05/12 11:10:54 gjb
+Re-added gjutil.c and gjutil.h to repository
+after deleting them
+
+Revision 1.2 1997/05/12 10:47:52 gjb
+Modified CVS header and log position
+
+*/
+#endif /* GJ_UTIL_H */
+
--- /dev/null
+/****************************************************************************
+
+ AGETBLOC - a routine to read an AMPS block file
+
+ Copyright: Geoffrey J. Barton (1992,1997)
+
+ email: geoff@ebi.ac.uk
+
+ Please see README for conditions of use.
+
+****************************************************************************
+
+History:
+
+15th November 1992 - ANSI C version - also uses GJ... routines.
+This version adapted with error messages for alscript.
+
+11th June 1992.
+Agetbloc: like getbloc, but does not require that every character read into
+the seqs structure is an alphabetic character. Also does not contain the
+option to convert the "sequences" read in into integer format
+
+getbloc: Read an AMPS style block file into the seqs array
+the nbloc aligned sequences are stored in positions 1-nbloc.
+
+This is a straight-ish translation of the fortran routine fbloc.f, hence
+the non-C like goto's...
+
+Sequence lengths are actual length +1 + 1. This allows position 0 to
+be reserved for future use, and preserves the '\0' for output.
+
+18/Feb/1993:Fix (i) to (i+1) in realloc. Spotted by RBR.
+
+ $Id: agetbloc.c,v 1.2 1998/09/17 16:54:58 geoff Exp $
+ $Log: agetbloc.c,v $
+ Revision 1.2 1998/09/17 16:54:58 geoff
+ Check consistency with archive
+
+
+*/
+
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+#include "array.h"
+#include "gjutil.h"
+
+
+int Agetbloc(FILE *bfile,struct seqdat *bloc,int *nbloc)
+
+{
+ int i,llen;
+ extern int MAXnseq, MAXslen, MAXilen, MAXtlen, MAXnbloc;
+ char *buff;
+ extern FILE *std_in,*std_out,*std_err;
+
+ char *idstart, *idend, *bstart, sident = 0;
+ int idlen,totseq = 0,k,j;
+
+ buff = (char *) GJmalloc(sizeof(char) * MAXtlen);
+
+l1:
+ buff = fgets(buff,MAXtlen,bfile);
+ if(buff == NULL){
+ fprintf(std_err,"Premature end of BLOCK FILE\n");
+ return 0;
+ }
+ if((idstart = strchr(buff,'>')) != NULL){
+ if(++totseq == MAXnbloc){
+ fprintf(std_err,
+ "Max Number of block file sequences exceeded: %d\n",
+ totseq);
+ fprintf(std_err,"Use MAX_NSEQ command to increase value");
+ return 0;
+ }
+ sident = 1;
+ idend = strchr(idstart,' ');
+ if(idend == NULL){
+ idend = strchr(idstart,'\0');
+ }
+ if(idend == NULL){
+ fprintf(std_err,"Error reading identifier:%s\n",idstart);
+ error("Exiting",1);
+ }
+ idlen = (idend - idstart) + 1;
+ bloc[totseq].id = (char *) malloc(sizeof(char) * idlen);
+ bloc[totseq].id = GJstrblank(bloc[totseq].id,idlen);
+ strncpy(bloc[totseq].id,idstart+1,idlen-1); /* don't copy the ">" symbol */
+ bloc[totseq].ilen = idlen-1;
+ bloc[totseq].id[idlen-1] = '\0';
+
+ bloc[totseq].tlen = strlen(idend)+1;
+ bloc[totseq].title = (char *) GJmalloc(sizeof(char) * bloc[totseq].tlen);
+ bloc[totseq].title = GJstrblank(bloc[totseq].title,bloc[totseq].tlen);
+ strcpy(bloc[totseq].title,idend);
+
+ bloc[totseq].seq = (char *) GJmalloc(sizeof(char) * MAXslen);
+ bloc[totseq].seq[0] = ' ';
+ goto l1;
+ } else if(sident){
+ if((idstart = strchr(buff,'*')) != NULL){
+ i = 0;
+ while((buff = fgets(buff,MAXtlen,bfile)) != NULL){
+ if(*idstart == '*'){
+/* fprintf(stdout,"Blocfile read: Length: %d\n",i);*/
+ ++i;
+ for(k=1;k<totseq+1;++k){
+ bloc[k].slen = i;
+ bloc[k].seq[i] = '\0';
+ bloc[k].seq = (char *) realloc(bloc[k].seq,sizeof(char)*(i+1)); /*i+1 fix suggested by rbr*/
+ }
+ *nbloc = totseq;
+ free(buff);
+ return 1;
+ }
+ bstart = idstart;
+ ++i;
+ if(i==MAXslen)error("Max Sequence length exceeded - use MAX_SEQ_LEN command to increase",1);
+ for(j=1;j<totseq+1;++j){
+ /*cope with short lines */
+/* if(!isalnum(*bstart)) *bstart = ' '; */
+ bloc[j].seq[i] = *bstart++;
+ }
+ }
+ fprintf(std_err,"No terminating * in blocfile\n");
+ return 0;
+ }else{
+ goto l1;
+ }
+ } else {
+ goto l1;
+ }
+}
+
--- /dev/null
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include "array.h"
+#include "gjutil.h"
+#include "version.h"
+
+/****************************************************************************
+
+ ALPS - Main routines for ALSCRIPT alignment formatting tool
+
+ Copyright: Geoffrey J. Barton (1992,1993,1997)
+
+ email: geoff@ebi.ac.uk
+
+All use of this program must cite: Barton, G. J. (1993), ALSCRIPT: A Tool to
+Format Multiple Sequence Alignments, Protein Engineering, Volume 6, No. 1,
+pp. 37-40.
+
+ Please see README file for details of limitations of use.
+
+ $Id: alps.c,v 1.3 1998/09/17 17:34:26 geoff Exp $
+ $Log: alps.c,v $
+ Revision 1.3 1998/09/17 17:34:26 geoff
+ Add the new README file and the LICENSE and RELEASE_NOTES
+
+ Revision 1.2 1998/09/17 16:54:58 geoff
+ Check consistency with archive
+
+
+****************************************************************************
+
+ Main routine for ALSCRIPT - formally called ALPS
+
+ alps: 11th June 1992.
+ Author: G. J. Barton
+
+ This file contains a set of subroutines that provide for flexible
+formatting of multiple sequence alignments in PostScript at
+publication quality.
+
+11-18th June 1992:
+
+1. Modified getbloc.c to Agetbloc.c. Agetbloc reads all characters
+(ie not just legal amino acid codes) from an AMPS blocfile format
+multiple alignment file.
+
+2. Wrote basic page scaling features to allow arbitrary page
+dimensions to be defined and to allow all x,y locations to be defined
+in terms of characters of the chosen pointsize. The number of
+residues per line and hence number of pages is determined by the
+pointsize. The page is divided into four areas. (a) The main region
+for plotting out the amino acid sequences. (b) A region to the left of
+a for plotting sequence identifier codes - the width of this region is
+set by variable Cidwidth. (c) A region above region a, and (d) a
+region below a. Regions c and d are initially set to zero size.
+Routine ALReserveSpace() allows a selected number of lines to be
+reserved above or below for plotting.
+
+3. Routines ALOutID and ALOutSeq written and multi-page plotting of
+the sequences sorted out. Still limited to one page in the vertical
+direction - toyed with the idea of writing single page PostScript and
+using PostScript to modify clipping boundaries like in the Poster demo
+program in the CookBook.
+
+4. Experiment with simple boxing facility - ALDrawSimpleBox(). This
+routine is incompatible with multi-page output since page breaks are
+unpredictable.
+
+5. Develop sophisticated (!) boxing facility - defines boxes in terms
+of TOP BOTTOM LEFT and RIGHT lines that may be defined around each
+character routine ALLineRes() implements this feature. The details of
+TOP etc for each character are stored in the unsigned char array
+"lines". This uses the rightmost four bits to encode LEFT RIGHT etc.
+Thus a line to the LEFT line is coded by decimal 1 (0001 binary), a
+full box by decimal 15 (1111 binary). ALBoxRes and ALBoxRegion
+written to allow boxing of arbitrary regions.
+
+6. The y coordinate sent to the low level ALLineRes routine
+refers directly to the index to the lines array. If you want to work in
+residue coordinate space, you need to flip the y coordinate before
+writing to the lines array (also the fill array). Routines ALSurroundChars
+and ALFillChars do this directly. Routines ALBoxRegion and ALFilRegion
+make use of the Yindx array which does the flip in a much cleaner and more
+elegant way. If I'd thought of it at the
+time, I would have used this in ALSurroundChars and ALFillChars as well!
+
+7. Now add capability to split alignments vertically as well as horizontally.
+There is now no limit on pointsize or number/length of sequences
+(in principle!). Next move on the page layout front will be to allow
+multiple blocks on a single page - this would permit alignments of a
+few sequences, or small fonts to make better use of paper!
+
+8. We now have most of the subroutines working OK. So time to tidy up the
+input. Add simple command parser to main programme - this is in two steps,
+step 1 reads the basic page layout commands, step 2 reads the boxing/shading
+font changing commands - Oh yes, added the font changing option as well.
+
+Current commands as follows:
+Commands are delimited by space, comma, or tab. Newline signals the end of a
+command. Unless otherwise stated, units are in characters of the default pointsize.
+For a full list see the file "alscript.doc".
+
+step 1 commands:
+
+BLOCK_FILE <input filename> %The name of the alignment file to format
+OUTPUT_FILE <file> %must have this near the beginning
+LANDSCAPE %Longest side horizontal
+PORTRAIT % vertical
+POINTSIZE <float> %Pointsize for page calculations and character spacing/default size.
+IDENT_WIDTH <integer> %Width allocated to identifiers.
+NUMBER_SEQS 1 || 0 %1=number identifiers, 0= no numbers (default).
+DEFINE_FONT <font number integer> <font name> <pointsize> %define a font to be referred to as font number.
+e.g.
+ DEFINE_FONT 0 Helvetica 10 %font 0 is Helvetica at 10 point
+ DEFINE_FONT 4 Times-Roman DEFAULT %font 4 is Times-Roman at the default pointsize.
+SETUP %signals end of step 1 commands.
+
+step 2 commands:
+
+RESERVE_SPACE_TOP <integer> %make space at the top of the alignment
+RESERVE_SPACE_BOTTOM <integer> % bottom DOES NOT WORK.
+SURROUND_CHARS <chars> <start_res> <start_seq> <end_res> <end_seq>
+ %surround (box) the characters chars within the ranges specified
+e.g.
+ SURROUND_CHARS PG 1 7 20 12 %surround P and G characters from residues 1 to 20 of sequences 7 to 12.
+ SURROUND_CHARS DEKHR ALL %surround D and E and K and R characters for the whole alignment.
+
+SHADE_CHARS <chars> <start_res> <start_seq> <end_res> <end_seq> <grey>
+ %similar syntax to SURROUND_CHARS with the addition of a grey value
+ % 0 = black, 1 = white. Note that shading is done before outputing the
+ % alignment.
+e.g.
+ SHADE_CHARS PG 1 7 20 12 0.5 % shade P and G in the range shown with a grey value of 0.5
+ SHADE_CHARS ILV ALL 0 % shade all I and L and V in black.
+
+FONT_CHARS <chars> <start_res> <start_seq> <end_res> <end_seq> <fontnumber>
+ %same syntax as SHADE_CHARS, only give the font number in place of grey.
+ %font numbers must first be defined with the DEFINE_FONT command above.
+FONT_RESIDUE sx sy font %allows setting of font for one residue (use with TEXT command)
+
+BOX_REGION <start_res> <start_seq> <end_res> <end_seq>
+ %draw a rectangular box around the characters within the defined range.
+SHADE_REGION <start_res> <start_seq> <end_res> <end_seq> <grey>
+ %shade the region by the chosed grey value.
+
+9. Add option to place arbitrary text string at a character location.
+e.g
+ TEXT 1 7 "SIGNAL PEPTIDE" %puts this text at sequence position 1 7.
+ %to change fonts use the FONT_RESIDUE or FONT_REGION commands.
+10. Add option to change fonts of identifiers.
+ID_FONT <sequence> <font>
+e.g.
+ ID_FONT 3 1 %sets font to 1 for sequence identifier 3
+ ID_FONT ALL 7 %uses font 7 for all identifiers
+
+19th June 1992:
+Still fiddling - must get back to some real work soon.
+Split alps away from the scanps program. Now have a separate main() for the routine.
+
+Add options to redefine all defaults: e.g. MAXslen (maximum sequence
+length etc.) Add arbitrary line drawing options: eg. LINE TOP 20 15 50
+draws a line along the top of the characters from residue 20 to 50 or
+sequence 15. Think about adding the option to change line thickness at
+different parts of the plot. Decide not to implement that option right
+now. Add option to include dummy sequences (ADD_SEQ)
+
+Check the programme compiles on the 386 PC. It does!
+Write documentation and brief report of the programme.
+
+22nd June 1992: Get back to some real work!
+
+19th October: Add SILENT_MODE Command and carriage returns to all .PS print statements.
+21st October: Add VERTICAL_SPACING command and ability to output multiple blocks per page.
+15th November: Add pipe options to alscript.c Tidy up file handling.
+
+6th December: Add Colour options.
+18th Jan 1993: Tidy up citations: modify Inverse chars to allow reversion.
+1 March 1993: Fix bug in colour option. Pass use_colour to ALDrawLines routine to
+ specify black for line colour. Previously, this was drawn in white
+ if coloured shading had been specified on the same characters.
+2 March 1993: Fix bug in colour option to output identifiers in black, not white!
+9 March 1993: Add NO_NUMBERS option to avoid printing residue numbers.
+24 March 1993: Add mask features.
+
+11 March 1994: At PERI - Add special text features - preliminary version only.
+ This permits strands and helices to be plotted as cylinders and arrows.
+ Special text features also permit triangles and circles etc etc. through
+ use of text command.
+
+23 May 1995: Numerous changes and additions over the last year - these
+include the option to colour backgrounds differently, ommission of
+idents on second and subsequent lines, helix, strand and other special
+characters, relative numbering, error checking of ranges on input,
+bounding box, screening, conservation colouring...
+
+
+*/
+
+#define TOKENS "\" ,\n\t" /* standard command token delimiters */
+#define POINT 72 /* Work in points throughout */
+#define C_ID_WIDTH 10 /* Default width for writing id's (in characters) */
+#define DEF_POINTSIZE 10.0
+#define X_OFFSET 40 /* offset for bottom lh corner of page (points) */
+#define Y_OFFSET 30
+#define DEF_YPOS 0 /* default first printinf position (character units) */
+#define LINE_WIDTH_FACTOR 0.05 /* Multiply pointsize by this to get linewidth */
+#define MAX_ADD_SEQ 10 /* Maximum number of ADD_SEQ commands allowed */
+
+/*#define MAX_S 10.5 max and min page side in inches */
+/*#define MIN_S 6.5 */
+/*#define MAXSIDE (MAX_S * POINT) Default Max and min dimensions of paper */
+/*#define MINSIDE (MIN_S * POINT) */
+
+#define SCREENSIZE 120 /* define default size for screen */
+
+
+int width = 0; /* max width and height of drawing area */
+int height = 0;
+char *tokens = TOKENS;
+
+float MAXside = 10.5 * POINT; /* values to hold Max and min page side in points */
+float MINside = 6.5 * POINT;
+
+int xoff = X_OFFSET;
+int yoff = Y_OFFSET;
+
+int Cidwidth = C_ID_WIDTH; /* width of id region in characters (left of sequence block)*/
+int Cseqwidth; /* width of sequence region in characters */
+int Cwidth; /* width/height of page in characters */
+int Cheight;
+int Xspace; /* spacing between characters in X */
+int Yspace; /* spacing between characters in Y */
+float XspaceFactor = 0.2; /* extra bit to add to horizontal character spacing */
+float YspaceFactor = 0.0; /* extra bit to add to vertical character spacing */
+float XshiftFactor = 0.12; /* *pointsize gives the shift for vertical lines */
+float YshiftFactor = 0.12; /* horizontal lines */
+
+int CseqTop = 0;
+int CseqVoffset = 0; /* vertical offset for sequence block (characters)*/
+int CtopSpace =0; /* extra space above sequence block (characters) */
+int CbottomSpace = 0; /* " " below " " " */
+int Csheet = 1; /* number of vertical splits in sequence block */
+
+int TVspace = 0; /* total vertical space reserved (characters)*/
+
+float Xshift = 0; /* offsets for drawing box lines */
+float Yshift = 0;
+float LineWidthFactor = LINE_WIDTH_FACTOR;
+
+float pointsize = DEF_POINTSIZE;
+int npage;
+
+int Vspacing = 0; /* vertical spacing in characters between blocks on a page */
+
+
+enum Orientations{
+ LANDSCAPE,
+ PORTRAIT
+};
+
+enum Spaces{
+ TOP,
+ BOTTOM
+};
+
+struct addseq {
+ int pos;
+ int num;
+};
+
+/* bit masks for the lines array */
+unsigned char LINE_LEFT = 1; /* mask for line at left of char */
+unsigned char LINE_RIGHT= 2; /* right */
+unsigned char LINE_TOP = 4; /* top */
+unsigned char LINE_BOTTOM=8; /* bottom */
+
+void ALOutId(struct seqdat *,int,unsigned char *colour,
+ unsigned char *,int,int,int,int, FILE *);
+void ALOutSeq(struct seqdat *,unsigned char **,
+ char ***,
+ unsigned char **,int ,unsigned char **,
+ unsigned char **text_colour,
+ unsigned char background_colour,
+ int ,int ,int ,int ,FILE *);
+void PSPutText(int,int,char *,FILE *);
+void PSPutChar(int,int,char,FILE *);
+void PSShowPage(FILE *);
+void ALSetFont(char *,int,FILE *);
+void ALCheckSinglePage(int ,int ,int );
+void ALSetPageLimits(void);
+void PSSetOrientation(int, FILE *);
+void PSPreamble(FILE *,int, int*, int screensize);
+void PSLandscape(FILE *);
+void PSPortrait(FILE *);
+void PSSetupPage(int,int,FILE *);
+void ALReserveSpace(int, int);
+void ALReportInfo(int, FILE *);
+char **ALCreateNumbers(int,int,int,int);
+char *ALCreateTicks(int,int,int);
+void ALOutTicks(char *,int,int,int,int,FILE *);
+void ALOutNumbers(char **,int,int,int,int,FILE *);
+void PSDrawBox(float,float,float,float,float,FILE *);
+void ALSimpleBox(int,int,int,int,float,FILE *);
+void PSline(float,float,float,float,FILE *);
+void ALLineChar(unsigned char **,int,int,unsigned char);
+void ALLineRes(unsigned char **,int,int,unsigned char);
+void ALDrawLines(unsigned char **,int,
+ unsigned char **left_line_colour,
+ unsigned char **right_line_colour,
+ unsigned char **top_line_colour,
+ unsigned char **bottom_line_colour,
+ int,int,int,int,int,int,FILE *);
+void ALBoxRes(unsigned char **,int,int);
+void ALDrawFills(signed char **,int ,unsigned char **,
+ int,int ,int,int ,int ,int, FILE *);
+void ALFilRes(signed char **,int,int,float);
+void ALBoxRegion(unsigned char **,int,int,int,int,int *);
+void ALFilRegion(signed char **,int ,int,int,int , int *,float);
+void ALSurroundChars(struct seqdat *,unsigned char **,int,int,int,int,int,char *);
+void ALShadeChars(struct seqdat *,signed char **,
+ int ,int,int,int,int,char *,float);
+void ALDefineFont(int,char *,float,FILE *);
+void echo(char *);
+void ALGetFourInt(int *,int *,int *,int *);
+void ALGetThreeInt(int *,int *,int *);
+void ALGetTwoInt(int *,int *);
+void ALFontChars(struct seqdat *,unsigned char **,
+ int,int,int,int,char *,unsigned char);
+void ALFontRegion(unsigned char **,int,int,int,int,unsigned char);
+void PSSetFont(unsigned char,FILE *);
+void ALDoLine(unsigned char **,int,int,int,int,int *);
+void ALSubChars(struct seqdat *,int,int,int,int,char,char);
+char ALChekSpace(const char *);
+void PSSetGrey(float,FILE *);
+void ALInverseChars(struct seqdat *,unsigned char **,int,int,int,int,char *);
+int Agetbloc(FILE *,struct seqdat *,int *);
+void ALDefineColour(int,char *coltype, float,float,float,FILE *);
+void ALSColChars(struct seqdat *,unsigned char **,
+ int,int,int,int,int,char *,unsigned char);
+void ALColourChars(struct seqdat *,unsigned char **,
+ int ,int,int,int,char *,unsigned char);
+void ALColRes(unsigned char **,int ,int , int );
+void PSSetColour(unsigned char,FILE *);
+void ALColRegion(unsigned char **,int,int,int,int,int *,unsigned char);
+void ALColRRegion(unsigned char **,int,int,int,int,unsigned char);
+void ckdd(struct seqdat *, int);
+int Ifound(struct seqdat *,int,int,char *,int);
+void ALSColMask(unsigned char **,unsigned char **,
+ int, int, int, int,int, unsigned char);
+void ALInverseMask(unsigned char **,unsigned char **,int, int, int, int);
+void ALFontMask(unsigned char **,unsigned char **,int, int, int, int,unsigned char);
+void ALShadeMask(unsigned char **,signed char **,int,int,int,int,int *, float);
+void ALGetAllRange(int *, int *, int *, int *,int ,int );
+void ALid_mask(unsigned char **,struct seqdat *,int, int, int, int, int, char *, char *);
+void ALfre_mask(unsigned char **mask,struct seqdat *,int, int, int, int, char *, char *);
+int Ifound(struct seqdat *,int,int,char *,int);
+void ALagree_mask(unsigned char **,struct seqdat *,int,int,int,int,int);
+void ALnot_mask(unsigned char **,int, int, int, int);
+void ALsub_mask(unsigned char **,struct seqdat *,int, int, int, int, char);
+void ALMask(unsigned char **,int, int, int, int);
+void ALConsMask(unsigned char **mask,
+int sx,int sy,int ex,int ey,int *consval,int conscut);
+void ALColourMask(unsigned char **,unsigned char **,int,int,int,int,unsigned char);
+void ALSurroundMask(unsigned char **,struct seqdat *,unsigned char **,int,int,int,int,int);
+int save_pir(struct seqdat *,int,FILE *);
+void ALDrawHorizLine(
+ float Xpos, /* bottom left hand corner of character posn */
+ float Ypos, /* */
+ float Xspace, /* horizontal width of character position */
+ float Yspace, /* vertical height of character position */
+ float width_fac,FILE *outf); /* proportion of vertical height that the line will fill */
+void ALDrawRightArrow(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawLeftArrow(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawUpArrow(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawDownArrow(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawCircle(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawPseudoEllipse(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,
+ FILE *outf);
+void ALDrawLeftSemiEnd(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawRightSemiEnd(float Xpos,float Ypos, float Xspace,
+ float Yspace, float width_fac,FILE *outf);
+void ALDrawRightHalfHorizLine(float Xpos,float Ypos,float Xspace,
+ float Yspace,float width_fac,FILE *outf);
+void ALDrawLeftHalfHorizLine(float Xpos,float Ypos,float Xspace,float
+ Yspace,float width_fac,FILE *outf);
+void PSmoveto(float x,float y,FILE *outf);
+void PSlineto(float x,float y,FILE *outf);
+void PSPutSpecialText(float Xpos,float Ypos,float Xspace,
+ float Yspace,char *text,unsigned char current_col,
+ unsigned char background_colour,
+ float YspaceFactor,FILE *outf);
+void PSstroke(FILE *outf);
+void PSnewpath(FILE *outf);
+void ALColourBackground(FILE *outf,int colour,int *background_region);
+void ALCheckLegalRange(int sx,int sy, int ex, int ey, int xmin, int ymin, int xmax, int ymax);
+void ALMakeCoilText(int sx, int sy, int ex, char ***texts);
+void ALMakeStrandText(int sx, int sy, int ex, char ***texts);
+void ALMakeHelixText(int sx, int sy, int ex, char ***texts);
+int ALrel(char *pos,int *relnum);
+int GJindex(char *str,char c);
+float mzcons(unsigned char *pos,int n);
+int *ALCalCons(struct seqdat *bloc,
+ int sx,int sy,int ex,int ey,int *ret_val,int len);
+int save_msf(struct seqdat *bloc,int nseq,FILE *msff);
+
+int alps(char *command_file,int silent, int pipe, int singlepage)
+{
+ FILE *cf,*inf,*outf,*pirf,*msff;
+ char *buff;
+ char *token;
+ char *charstring;
+ struct seqdat *bloc;
+ struct seqdat *temp;
+ int orientation;
+ extern int MAXtlen,MAXnbloc;
+ extern int MAXilen,precis,MAXslen;
+ int nseq;
+ int start_aa;
+ int start_seq;
+ int i,j,k;
+ int totalpage;
+ char **numbers;
+ char *ticks;
+ unsigned char **lines;
+#ifdef VAX
+ char **fills;
+#else
+ signed char **fills;
+#endif
+ unsigned char **fonts;
+ unsigned char *idfonts;
+ unsigned char **inverse;
+ char ***texts;
+ int *Yindx;
+ int number_seqs = 0;
+
+ int Xtemp,Ytemp;
+ int sx,sy,ex,ey;
+
+ int fnum;
+ char *fname;
+ float fpoint;
+ int rowsiz;
+
+ char oldchar,newchar;
+
+ struct seqdat *addbloc;
+ struct addseq *adds;
+ int nadds;
+ int natot;
+ int l;
+ int do_ticks = 0;
+ int number_int = 10;
+ int pirsave = 0;
+ int msfsave = 0;
+
+ int nbp,nspage; /*counter and total for number of blocks per page */
+ int lastY;
+ int nbpage;
+
+ /* bounding_box - allows explicit fiddling with the bounding_box */
+ int *bounding_box;
+
+ /* colours */
+ float red,green,blue;
+ unsigned char **s_colour; /*colour number for shading */
+ unsigned char **c_colour; /*colour number for characters */
+ /* new 4th Oct 1994 */
+ unsigned char *id_colour; /*colour for identifiers */
+ unsigned char **left_line_colour; /*colours for lines */
+ unsigned char **right_line_colour;
+ unsigned char **top_line_colour;
+ unsigned char **bottom_line_colour;
+ unsigned char **text_colour; /*colour for text */
+ char *colname;
+
+ int background_colour = 99; /*set background to white */
+ int *background_region; /* use this to set the region for background colouring */
+
+ int number_colour = 100; /* colour for numbers along top of plot */
+ /* end new */
+ char *tstring;
+
+ int use_colour; /* flag set if the define_colour command is seen */
+ int res_numbers;
+
+ int msl;
+
+ /* min and max ranges in the sequence alignment */
+ int xmin, ymin, xmax, ymax;
+
+ /* masking variables */
+ unsigned char **mask;
+ char *illegal;
+ char *legal;
+ int id_cut;
+
+ /* screen size variable - for printer screen */
+ int screensize = SCREENSIZE;
+
+ /* set up relative numbering - allows commands to follow
+ a specific sequence numbering
+ firstres defines the number of the first residue */
+ int relative_seq = 0;
+ int firstres = 0;
+ int i1;
+ int *relnum;
+ /* temp relnum */
+ int *trelnum;
+
+ /* flag for idents */
+ int id_only_on_first = 0;
+ int tempCseqwidth;
+ int tempCidwidth;
+
+ /* conservation values */
+ int *consval; /* array */
+ int conscut; /* cutoff */
+
+ /* counter for add_seq errors */
+ int adderr;
+
+ /* file streams */
+ extern FILE *std_in, *std_out, *std_err;
+
+ /* By default read and write to stdin and stdout */
+ outf = std_out;
+ inf = std_in;
+
+
+ /* default is no colour */
+ use_colour = 0;
+
+ /* if set, residue numbers are output */
+ res_numbers = 1;
+
+
+
+ /* set the bounding box to default values for portrait A4*/
+ bounding_box = (int *) GJmalloc(sizeof(int) * 4);
+ bounding_box[0] = 0;
+ bounding_box[1] = 0;
+ bounding_box[2] = 590;
+ bounding_box[3] = 826;
+
+ /* set the background colour region equal to the default bounding box */
+ background_region = (int *) GJmalloc(sizeof(int) * 4);
+ background_region[0] = 0;
+ background_region[1] = 0;
+ background_region[2] = 590;
+ background_region[3] = 826;
+
+ /* initialise the mask */
+ mask = NULL;
+ illegal = NULL;
+ legal = NULL;
+
+ adds = (struct addseq *) malloc(sizeof(struct addseq) * (MAX_ADD_SEQ+1));
+ nadds = 0;
+
+
+ fname = (char *) malloc(sizeof(char)*MAXtlen);
+
+ buff = (char *) malloc(MAXtlen * sizeof(char));
+ if(buff == NULL)error("No Space for buff",1);
+
+ if((cf = fopen(command_file,"r")) == NULL){
+ error("Cannot open ALSCRIPT command file",0);
+ return 0;
+ }
+
+ /*
+ This is a little inelegant at the moment - when I get the generalized
+ parser finished this could be cleaned up substantially
+ */
+
+ /* main loop to read commands */
+ if(pipe){
+ /* Alscript is being used as a pipe so write preamble to std_out */
+ outf = std_out;
+ PSPreamble(outf,singlepage,bounding_box,screensize);
+ }
+
+ if(!silent)fprintf(std_err,"Starting ALSCRIPT\n");
+
+ while(buff = fgets(buff,MAXtlen,cf)){
+ if(!silent)echo(buff);
+ if(buff == NULL) continue;
+ token = strtok(buff,TOKENS);
+ if(token == NULL) continue;
+ token = GJstoup(token);
+ if(*token == '#'){
+ if(!silent)echo("Comment\n");
+ }else if(strcmp(token,"SILENT_MODE")==0){
+ if(!silent){
+ silent=1;
+ }else{
+ silent=0;
+ }
+ }else if(strcmp(token,"MAX_INPUT_LEN")==0){
+ if(!silent) fprintf(std_err,"Max input len was:\t%d\n",MAXtlen);
+ token = strtok(NULL,TOKENS);
+ MAXtlen = atoi(token);
+ if(!silent)fprintf(std_err,"Max input len now:\t%d\n",MAXtlen);
+ }else if(strcmp(token,"MAX_NSEQ")==0){
+ if(!silent)fprintf(std_err,"Max No. of sequences was:\t%d\n",MAXnbloc);
+ token = strtok(NULL,TOKENS);
+ MAXnbloc = atoi(token);
+ MAXtlen = MAXnbloc;
+ fprintf(std_err,"Max No. of sequences now:\t%d\n",MAXnbloc);
+ }else if(strcmp(token,"MAX_ILEN")==0){
+ if(!silent)fprintf(std_err,"Max Identifier length was:\t%d\n",MAXilen);
+ token = strtok(NULL,TOKENS);
+ MAXilen = atoi(token);
+ if(!silent)fprintf(std_err,"Max Identifier length now:\t%d\n",MAXilen);
+ }else if(strcmp(token,"PRECISION")==0){
+ token = strtok(NULL,TOKENS);
+ precis = atoi(token);
+ }else if(strcmp(token,"MAX_SEQ_LEN")==0){
+ if(!silent)fprintf(std_err,"Max Sequence Length was:\t%d\n",MAXslen);
+ token = strtok(NULL,TOKENS);
+ MAXslen = atoi(token);
+ if(!silent)fprintf(std_err,"Max Sequence Length now:\t%d\n",MAXslen);
+ }else if(strcmp(token,"X_OFFSET")==0){
+ if(!silent)fprintf(std_err,"X offset was:\t%d\n",xoff);
+ token = strtok(NULL,TOKENS);
+ xoff = atoi(token);
+ if(!silent)fprintf(std_err,"X offset now:\t%d\n",xoff);
+ }else if(strcmp(token,"Y_OFFSET")==0){
+ if(!silent)fprintf(std_err,"Y offset was:\t%d\n",yoff);
+ token = strtok(NULL,TOKENS);
+ yoff = atoi(token);
+ if(!silent)fprintf(std_err,"Y offset now:\t%d\n",yoff);
+ }else if(strcmp(token,"MAX_SIDE")==0){
+ if(!silent)fprintf(std_err,"Longest page side was:\t%.2f inches\n",MAXside/POINT);
+ token = strtok(NULL,TOKENS);
+ MAXside = POINT * atof(token);
+ if(!silent)fprintf(std_err,"Longest page side now:\t%.2f inches\n",MAXside/POINT);
+ }else if(strcmp(token,"MIN_SIDE")==0){
+ if(!silent)fprintf(std_err,"Shortest page side was:\t%.2f inches\n",MINside/POINT);
+ token = strtok(NULL,TOKENS);
+ MINside = POINT * atof(token);
+ if(!silent)fprintf(std_err,"Shortest page side now:\t%.2f inches\n",MINside/POINT);
+ }else if(strcmp(token,"LINE_WIDTH_FACTOR")==0){
+ if(!silent)fprintf(std_err,"Line width multiplication factor was:\t%f\n",LineWidthFactor);
+ token = strtok(NULL,TOKENS);
+ LineWidthFactor = atof(token);
+ if(!silent)fprintf(std_err,"Line width multiplication factor now:\t%f\n",LineWidthFactor);
+ }else if(strcmp(token,"SCREENSIZE")==0){
+ if(!silent)fprintf(std_err,"Screensize was:\t%d\n",screensize);
+ token = strtok(NULL,TOKENS);
+ screensize = atoi(token);
+ if(!silent)fprintf(std_err,"Screensize now:\t%d\n",screensize);
+ }else if(strcmp(token,"BLOCK_FILE") == 0){
+ token = strtok(NULL,TOKENS);
+ if(!pipe){
+ inf = fopen(token,"r");
+ if(inf == NULL)error("Cannot open BLOCK_FILE\n",1);
+ }
+ }else if(strcmp(token,"OUTPUT_FILE")==0){
+ token = strtok(NULL,TOKENS);
+ if(!pipe){
+ outf = fopen(token,"w");
+ if(outf == NULL)error("Cannot open OUTPUT_FILE\n",1);
+ PSPreamble(outf,singlepage,bounding_box,screensize);
+ }
+ }else if(strcmp(token,"PIR_SAVE")==0){
+ token = strtok(NULL,TOKENS);
+ pirf = fopen(token,"w");
+ if(pirf == NULL)error("Cannot open PIR_SAVE file\n",1);
+ pirsave = 1;
+ }else if(strcmp(token,"MSF_SAVE")==0){
+ token = strtok(NULL,TOKENS);
+ msff = fopen(token,"w");
+ if(msff == NULL)error("Cannot open MSF_SAVE file\n",1);
+ msfsave = 1;
+ }else if(strcmp(token,"LANDSCAPE")==0){
+ orientation = LANDSCAPE;
+ }else if(strcmp(token,"PORTRAIT")==0){
+ orientation = PORTRAIT;
+
+ }else if(strcmp(token,"POINTSIZE")==0){
+ token = strtok(NULL,TOKENS);
+ pointsize = atof(token);
+ }else if(strcmp(token,"IDENT_WIDTH")==0){
+ token = strtok(NULL,TOKENS);
+ Cidwidth = atoi(token);
+ if(Cidwidth > MAXilen)error("INCREASE MAX_ID_LEN value",1);
+ }else if(strcmp(token,"NUMBER_SEQS")==0){
+ number_seqs=1;
+ }else if(strcmp(token,"X_SPACE_FACTOR")==0){
+ if(!silent)fprintf(std_err,"Was:\t%.2f\n",XspaceFactor);
+ token = strtok(NULL,TOKENS);
+ XspaceFactor = atof(token);
+ if(!silent)fprintf(std_err,"Now Is:\t%.2f\n",XspaceFactor);
+ }else if(strcmp(token,"Y_SPACE_FACTOR")==0){
+ if(!silent)fprintf(std_err,"Was:\t%.2f\n",YspaceFactor);
+ token = strtok(NULL,TOKENS);
+ YspaceFactor = atof(token);
+ }else if(strcmp(token,"X_SHIFT_FACTOR")==0){
+ if(!silent)fprintf(std_err,"Was:\t%.2f\n",XshiftFactor);
+ token = strtok(NULL,TOKENS);
+ XshiftFactor = atof(token);
+ if(!silent)fprintf(std_err,"Now Is:\t%.2f\n",XshiftFactor);
+ }else if(strcmp(token,"Y_SHIFT_FACTOR")==0){
+ if(!silent)fprintf(std_err,"Was:\t%.2f\n",YshiftFactor);
+ token = strtok(NULL,TOKENS);
+ YshiftFactor = atof(token);
+ if(!silent)fprintf(std_err,"Now Is:\t%.2f\n",YshiftFactor);
+ }else if(strcmp(token,"VERTICAL_SPACING")==0){
+ if(!silent)fprintf(std_err,"Was:\t%d\n",Vspacing);
+ token = strtok(NULL,TOKENS);
+ Vspacing = (int) atol(token);
+ if(!silent)fprintf(std_err,"Now Is:\t%d\n",Vspacing);
+ }else if(strcmp(token,"DEFINE_FONT")==0){
+ token = strtok(NULL,TOKENS);
+ fnum = atoi(token); /* font number */
+ token = strtok(NULL,TOKENS); /* font name */
+ fname = strcpy(fname,token);
+ token = strtok(NULL,TOKENS);
+ if(strcmp(token,"DEFAULT")==0){
+ fpoint = pointsize;
+ }else if(strcmp(token,"REL")==0){
+ token = strtok(NULL,TOKENS);
+ fpoint = pointsize * atof(token);
+ }else{
+ fpoint = atof(token);
+ }
+ ALDefineFont(fnum,fname,fpoint,outf);
+ }else if(strcmp(token,"DEFINE_COLOUR")==0 || strcmp(token,"DEFINE_COLOR")==0){
+ /* new style
+ define_colour <colour_number | colour type> colour_name r g b <colour_number> */
+ /* e.g. define_colour 1 1.0 1.0 1.0
+ (same as before)
+ define_colour rgb red 1.0 0.0 0.0 7
+ (define an rgb colour (red,green,blue),colour_number)
+ define_colour hsb red 1.0 0.0 0.0 9
+ (define an hsb colour (hue,sat,bright),colour_number)
+ currently can use either hsb OR rgb, but not mix them
+ */
+ use_colour = 1;
+ token = strtok(NULL,TOKENS);
+ tstring = GJstoupper(token);
+ if(strcmp(tstring,"RGB")==0 || strcmp(tstring,"HSB")==0){
+ token = strtok(NULL,TOKENS);
+ colname = GJstrdup(token);
+ token = strtok(NULL,TOKENS);
+ red = atof(token);
+ token = strtok(NULL,TOKENS);
+ green = atof(token);
+ token = strtok(NULL,TOKENS);
+ blue = atof(token);
+ token = strtok(NULL,TOKENS);
+ fnum = atoi(token);
+ }else{
+ fnum = atoi(token); /* colour number */
+ token = strtok(NULL,TOKENS);
+ red = atof(token);
+ token = strtok(NULL,TOKENS);
+ green = atof(token);
+ token = strtok(NULL,TOKENS);
+ blue = atof(token);
+
+ }
+ /* for now just ignore the colour name - we'll allow use of this later */
+ ALDefineColour(fnum,tstring,red,green,blue,outf);
+ GJfree(tstring);
+ }else if(strcmp(token,"ADD_SEQ")==0){
+ token = strtok(NULL,TOKENS);
+ adds[nadds].pos = atoi(token);
+ token = strtok(NULL,TOKENS);
+ adds[nadds].num = atoi(token);
+ ++nadds;
+ }else if(strcmp(token,"DO_TICKS")==0){
+ do_ticks=1;
+ }else if(strcmp(token,"NUMBER_INT")==0){
+ token = strtok(NULL,TOKENS);
+ number_int = atoi(token);
+ if(!silent)fprintf(std_err,"Sequence Numbering interval now:\t%d\n",number_int);
+ }else if(strcmp(token,"NO_NUMBERS")==0){
+ res_numbers = 0;
+ }else if(strcmp(token,"NUMBER_COLOUR")==0){
+ token = strtok(NULL,TOKENS);
+ number_colour = atoi(token);
+ }else if(strcmp(token,"SINGLE_PAGE")==0){
+ /* if this is set, then alscript assumes a single page -
+ just adds a bounding box for now*/
+ if(!silent)fprintf(std_err,"Single page was: %d\n",singlepage);
+ singlepage = 1;
+ if(!silent)fprintf(std_err,"Single page now: %d\n",singlepage);
+ }else if(strcmp(token,"BOUNDING_BOX")==0){
+ ALGetFourInt(&bounding_box[0],&bounding_box[1],
+ &bounding_box[2],&bounding_box[3]);
+ if(!silent){
+ fprintf(std_err,"Bounding box set to: %d %d %d %d\n",
+ bounding_box[0],bounding_box[1],
+ bounding_box[2],bounding_box[3]);
+ }
+ }else if(strcmp(token,"BACKGROUND_REGION") == 0){
+ /* set the region of the background to colour */
+ ALGetFourInt(&background_region[0],&background_region[1],
+ &background_region[2],&background_region[3]);
+ if(!silent){
+ fprintf(std_err,"Background region set to: %d %d %d %d\n",
+ background_region[0],background_region[1],
+ background_region[2],background_region[3]);
+ }
+ }else if(strcmp(token,"ID_ONLY_ON_FIRST_LINE")==0){
+ /* flag to indicate identifiers only on first line!*/
+ id_only_on_first = 1;
+ }else if(strcmp(token,"BACKGROUND_COLOUR") ==0){
+ /* set the colour for background defined by background_region */
+ token = strtok(NULL,TOKENS);
+ background_colour = atoi(token);
+ }else if(strcmp(token,"SETUP")==0){
+ if(!silent)fprintf(std_err,"Initial Commands read - Establishing basic settings\n");
+ break;
+ }else{
+ fprintf(std_err,"%s",buff);
+ error("Unrecognised Step 1 command",1);
+
+ }
+ }
+
+ if(outf == std_out){
+ if(!silent)fprintf(std_err,"Output file undefined - will write to stdout\n");
+ }
+
+
+ if(inf == std_in){
+ if(!silent)fprintf(std_err,"Block file undefined - will read from stdin\n");
+ }
+
+ bloc = (struct seqdat *) malloc(sizeof(struct seqdat) * MAXnbloc);
+ mcheck((char *) bloc,"Cannot get space for bloc");
+ if(!Agetbloc(inf,bloc,&nseq))error("Cannot read bloc file",1);
+
+ ckdd(bloc,nseq);
+
+ if(pirsave){
+ if(!save_pir(bloc,nseq,pirf))error("Cannot write to pirf file",1);
+ exit(0);
+ }
+
+ if(msfsave){
+ if(!save_msf(bloc,nseq,msff))error("Cannot write to msf file",1);
+ exit(0);
+ }
+
+ /* Initialise relnum to 1..n*/
+ /* relnum = (int *) GJmalloc(sizeof(int) * MAXslen); */
+ /* for(i=1;i<MAXslen;++i){ */
+ /* relnum[i]=i; */
+ /* } */
+
+ /* Initialise relnum to 1..n*/
+ /* 29 July 1998 - allocate relnum to MAXslen *2. with pointer in the middle */
+
+ trelnum = (int *) GJmalloc(sizeof(int) * MAXslen * 2);
+ for(i=1;i<(MAXslen +1);++i){
+ trelnum[i]=0;
+ }
+ relnum = &trelnum[MAXslen];
+ for(i=1;i<MAXslen;++i){
+ relnum[i] = i;
+ }
+
+ /* do a check of the adds array to make sure there are no out of bounds
+ requests
+ */
+ adderr = 0;
+ for(i=0;i<nadds;++i){
+ if(adds[i].pos > nseq){
+ ++adderr;
+ fprintf(std_err,"Error in ADD_SEQ request: %d %d\n",adds[i].pos,adds[i].num);
+ fprintf(std_err,"%d is greater than the number of sequences in the block file(%d)\n",adds[i].pos,nseq);
+ fprintf(std_err,"Please see the manual for help on using ADD_SEQ\n");
+ }
+ }
+ if(adderr > 0){
+ fprintf(std_err,"%d ADD_SEQ errors - exiting\n",adderr);
+ exit(-1);
+ }
+
+
+ if(nadds > 0){
+ if(!silent)fprintf(std_err,"Making extra space for %d additions\n",nadds);
+ natot = 0;
+ for(i=0;i<nadds;++i){
+ natot += adds[i].num;
+ }
+ if(!silent)fprintf(std_err,"Total of %d extra sequences\n",natot);
+ addbloc = (struct seqdat *) malloc(sizeof(struct seqdat) *(nseq+natot+1));
+ i = 0;
+ j = 0;
+ k = 0;
+ while(i < nseq+1){
+ if(k < nadds && i==adds[k].pos){
+ /* reserve space for the new sequences and set to blank*/
+ for(l=0;l<adds[k].num;++l){
+ ++j;
+ addbloc[j].id = (char *) malloc(sizeof(char) * MAXilen);
+ addbloc[j].id = GJstrblank(addbloc[j].id,MAXilen);
+ addbloc[j].seq = (char *) malloc(sizeof(char) * (bloc[1].slen+1));
+ addbloc[j].seq = GJstrblank(addbloc[j].seq,bloc[1].slen+1);
+ }
+ ++k;
+ }else{
+ /* just copy the pointers to the bloc sequences */
+ ++i;
+ ++j;
+ if(i<nseq+1){
+ addbloc[j].id = bloc[i].id;
+ addbloc[j].title = bloc[i].title;
+ addbloc[j].seq = bloc[i].seq;
+ }
+ }
+ }
+ addbloc[1].slen = bloc[1].slen;
+ temp = bloc;
+ bloc = addbloc;
+ addbloc = temp;
+ nseq += natot;
+ if(!silent)fprintf(std_err,"Total number of sequences now: %d\n",nseq);
+ }
+ msl = bloc[1].slen-2; /* maximum sequence length defined */
+
+ xmin = 1; ymin = 1; xmax = msl+1; ymax = nseq;
+
+ PSSetupPage(orientation,1,outf);
+ ALSetPageLimits();
+
+ CtopSpace = 2; /* set top 2 lines for numbers */
+
+ /* now set up the bounding box and background
+ based on current values of xoff, yoff, width and height
+ these can be overridden by step 2 commands
+
+ bounding_box[0] = 0;
+ bounding_box[1] = 0;
+ bounding_box[2] = width;
+ bounding_box[3] = height;
+ */
+
+ /* set the background colour region equal to the default bounding box
+ background_region[0] = bounding_box[0];
+ background_region[1] = bounding_box[1];
+ background_region[2] = bounding_box[2];
+ background_region[3] = bounding_box[3];
+ */
+
+ if(background_colour != 99)ALColourBackground(outf,background_colour,background_region);
+
+
+
+/* TVspace = nseq;*/
+/* ALReserveSpace(TOP,2);*/
+
+
+ /* allocate to +1 size to allow indexing from 1...max */
+ /* lines array - stored info on where to draw lines */
+ lines = uchararr(bloc[1].slen+1,nseq+1);
+ if(lines == NULL)error("No space for lines array\n",1);
+ GJUCinit(lines,bloc[1].slen+1,nseq+1,0);
+
+ /* array holds grey value for each character fill*/
+ fills = chararr(bloc[1].slen+1,nseq+1);
+ if(fills == NULL)error("No space for fills array\n",1);
+ GJCinit(fills,bloc[1].slen+1,nseq+1,-1);
+
+ /* allocate to +1 size to allow indexing from 1...max */
+ /* fonts array - stores a number representing the font to use for each residue */
+ /* default font is font 0 */
+ fonts = uchararr(bloc[1].slen+1,nseq+1);
+ if(fonts == NULL)error("No space for fonts array\n",1);
+ GJUCinit(fonts,bloc[1].slen+1,nseq+1,0);
+
+ /* inverse indicates whether the the text at i j should be printed in white */
+ inverse = uchararr(bloc[1].slen+1,nseq+1);
+ if(inverse == NULL)error("No space for inverse array\n",1);
+ GJUCinit(inverse,bloc[1].slen+1,nseq+1,0);
+
+ /* colour arrays - define the colour to use for shading and lettering */
+ /* default colour numbers are 99 for white and 100 for black */
+ s_colour = uchararr(bloc[1].slen+1,nseq+1); /* shading colour */
+ if(s_colour == NULL)error("No space for s_colour array\n",1);
+ GJUCinit(s_colour,bloc[1].slen+1,nseq+1,background_colour);
+ c_colour = uchararr(bloc[1].slen+1,nseq+1); /* character colour */
+ if(c_colour == NULL)error("No space for c_colour array\n",1);
+ GJUCinit(c_colour,bloc[1].slen+1,nseq+1,100);
+ left_line_colour = uchararr(bloc[1].slen+1,nseq+1); /* left line colour */
+ if(left_line_colour == NULL)error("No space for left_line_colour array\n",1);
+ GJUCinit(left_line_colour,bloc[1].slen+1,nseq+1,100);
+ right_line_colour = uchararr(bloc[1].slen+1,nseq+1); /* right line colour */
+ if(right_line_colour == NULL)error("No space for right_line_colour array\n",1);
+ GJUCinit(right_line_colour,bloc[1].slen+1,nseq+1,100);
+ top_line_colour = uchararr(bloc[1].slen+1,nseq+1); /* top line colour */
+ if(top_line_colour == NULL)error("No space for top_line_colour array\n",1);
+ GJUCinit(top_line_colour,bloc[1].slen+1,nseq+1,100);
+ bottom_line_colour = uchararr(bloc[1].slen+1,nseq+1); /* bottom line colour */
+ if(bottom_line_colour == NULL)error("No space for bottom_line_colour array\n",1);
+ GJUCinit(bottom_line_colour,bloc[1].slen+1,nseq+1,100);
+ text_colour = uchararr(bloc[1].slen+1,nseq+1); /* text colour */
+ if(text_colour == NULL)error("No space for text_colour array\n",1);
+ GJUCinit(text_colour,bloc[1].slen+1,nseq+1,100);
+
+ /* holds font information for each identifier */
+ idfonts = (unsigned char *) malloc(sizeof(unsigned char) * nseq+1);
+ for(i=0;i<nseq+1;++i){
+ idfonts[i]= (unsigned char) 0;
+ }
+ /* holds colour information for each identifier */
+ id_colour = (unsigned char *) GJmalloc(sizeof(unsigned char) * nseq+1);
+ for(i=0;i<nseq+1;++i){
+ id_colour[i]= (unsigned char) 100;
+ }
+ /* holds conservation values */
+ consval = (int *) GJmalloc(sizeof(int) * bloc[1].slen);
+ GJIinit(consval,bloc[1].slen,0);
+
+
+ /* array holds pointers to text strings at each [i][j] point*/
+ texts = (char ***) malloc(sizeof(char **) * (bloc[1].slen+1));
+ rowsiz = sizeof(char *) * (nseq + 1);
+ for(k=0;k<bloc[1].slen+1;++k){
+ texts[k] = (char **) malloc(rowsiz);
+ for(i=0;i<(nseq+1);++i){
+ texts[k][i]=NULL;
+ }
+ }
+
+ Yindx = (int *) malloc(sizeof(int)*(nseq+1));
+ for(i=1,j=nseq;i<nseq+1;++i,--j){
+ Yindx[i] = j;
+ }
+
+ numbers = ALCreateNumbers(0,number_int,bloc[1].slen,4);
+ ticks = ALCreateTicks(0,number_int,bloc[1].slen);
+ CseqTop = nseq;
+
+ while(buff = fgets(buff,MAXtlen,cf)){
+ if(buff == NULL) break;
+ if(!silent)echo(buff);
+ token = strtok(buff,TOKENS);
+ if(token == NULL) break;
+ token = GJstoup(token);
+ if(*token == '#'){
+ if(!silent)echo("Comment\n");
+ }else if(strcmp(token,"SILENT_MODE")==0){
+ if(!silent){
+ silent=1;
+ }else{
+ silent=0;
+ }
+/* This block has moved to the MODE 1 section
+ }else if(strcmp(token,"BOUNDING_BOX")==0){
+ ALGetFourInt(&bounding_box[0],&bounding_box[1],
+ &bounding_box[2],&bounding_box[3]);
+
+ }else if(strcmp(token,"BACKGROUND_REGION") == 0){
+ set the region of the background to colour
+ ALGetFourInt(&background_region[0],&background_region[1],
+ &background_region[2],&background_region[3]);
+*/
+ }else if(strcmp(token,"RESERVE_SPACE_TOP")==0){
+ token = strtok(NULL,TOKENS);
+ ALReserveSpace(TOP,atoi(token));
+ }else if(strcmp(token,"RESERVE_SPACE_BOTTOM")==0){
+ token = strtok(NULL,TOKENS);
+ ALReserveSpace(BOTTOM,atoi(token));
+ CseqTop = nseq + CbottomSpace;
+ }else if(strcmp(token,"SURROUND_CHARS")==0){
+ token = strtok(NULL,TOKENS);
+ charstring = token;
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALSurroundChars(bloc,lines,relnum[sx],sy,relnum[ex],ey,nseq,charstring);
+ }else if(strcmp(token,"SHADE_CHARS")==0){
+ token = strtok(NULL,TOKENS);
+ charstring = token;
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALShadeChars(bloc,fills,relnum[sx],sy,relnum[ex],ey,nseq,charstring,(float)atof(token));
+ }else if(strcmp(token,"FONT_CHARS")==0){
+ token = strtok(NULL,TOKENS);
+ charstring = token;
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALFontChars(bloc,fonts,relnum[sx],sy,relnum[ex],ey,charstring,atoi(token));
+ }else if(strcmp(token,"CCOL_CHARS")==0){ /* colour characters */
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ token = strtok(NULL,TOKENS);
+ charstring = token;
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColourChars(bloc,c_colour,relnum[sx],sy,relnum[ex],ey,charstring,atoi(token));
+ }else if(strcmp(token,"SCOL_CHARS")==0){ /* colour character backgrounds */
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ token = strtok(NULL,TOKENS);
+ charstring = token;
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALSColChars(bloc,s_colour,relnum[sx],sy,relnum[ex],ey,nseq,charstring,(unsigned char) atoi(token));
+ }else if(strcmp(token,"INVERSE_CHARS")==0){
+ token = strtok(NULL,TOKENS);
+ charstring = token;
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALInverseChars(bloc,inverse,relnum[sx],sy,relnum[ex],ey,charstring);
+ }else if(strcmp(token,"SUB_CHARS")==0){
+ token = strtok(NULL,TOKENS);
+ if(strcmp(token,"ALL")==0){
+ token = strtok(NULL,TOKENS);
+ oldchar = ALChekSpace(token);
+ token = strtok(NULL,TOKENS);
+ newchar = ALChekSpace(token);
+ ALSubChars(bloc,1,1,bloc[1].slen-1,nseq,oldchar,newchar);
+ }else{
+ sx = atoi(token);
+ ALGetThreeInt(&sy,&ex,&ey);
+ token = strtok(NULL,TOKENS);
+ oldchar = ALChekSpace(token);
+ token = strtok(NULL,TOKENS);
+ newchar = ALChekSpace(token);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALSubChars(bloc,relnum[sx],sy,relnum[ex],ey,oldchar,newchar);
+ }
+ }else if(strcmp(token,"SUB_ID")==0){
+ token = strtok(NULL,"\"");
+ sx = atoi(token);
+ token = strtok(NULL,"\"");
+ ALCheckLegalRange(xmin,1,xmax,sx,xmin,ymin,xmax,ymax);
+ bloc[sx].id = (char *) realloc(bloc[sx].id,sizeof(char)*(strlen(token)+1));
+ bloc[sx].id = strcpy(bloc[sx].id,token);
+ }else if(strcmp(token,"FONT_REGION")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ token = strtok(NULL,TOKENS);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALFontRegion(fonts,relnum[sx],sy,relnum[ex],ey,atoi(token));
+ }else if(strcmp(token,"FONT_RESIDUE")==0){
+ token = strtok(NULL,TOKENS);
+ sx = atoi(token);
+ token=strtok(NULL,TOKENS);
+ sy = atoi(token);
+ token=strtok(NULL,TOKENS);
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax);
+ ALFontRegion(fonts,relnum[sx],sy,relnum[sx],sy,atoi(token));
+ }else if(strcmp(token,"LINE")==0){
+ token = strtok(NULL,TOKENS);
+ if(strcmp(token,"TOP")==0){
+ ALGetThreeInt(&sx,&sy,&ex);
+ if(ex > bloc[1].slen-1) ex = bloc[1].slen-1;
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax);
+ ALDoLine(lines,relnum[sx],sy,relnum[ex],LINE_TOP,Yindx);
+ }else if(strcmp(token,"BOTTOM")==0){
+ ALGetThreeInt(&sx,&sy,&ex);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax);
+ if(ex > bloc[1].slen-1) ex = bloc[1].slen-1;
+ ALDoLine(lines,relnum[sx],sy,relnum[ex],LINE_BOTTOM,Yindx);
+ }else if(strcmp(token,"LEFT")==0){
+ ALGetThreeInt(&sx,&sy,&ey);
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],ey,xmin,ymin,xmax,ymax);
+ if(ey > nseq) ey = nseq;
+ ALDoLine(lines,relnum[sx],sy,ey,LINE_LEFT,Yindx);
+ }else if(strcmp(token,"RIGHT")==0){
+ ALGetThreeInt(&sx,&sy,&ey);
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],ey,xmin,ymin,xmax,ymax);
+ if(ey > nseq) ey = nseq;
+ ALDoLine(lines,relnum[sx],sy,ey,LINE_RIGHT,Yindx);
+ }else{
+ error("Illegal LINE command\n",1);
+ }
+ }else if(strcmp(token,"BOX_REGION")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALBoxRegion(lines,relnum[sx],sy,relnum[ex],ey,Yindx);
+ }else if(strcmp(token,"SHADE_REGION")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALFilRegion(fills,relnum[sx],sy,relnum[ex],ey,Yindx,(float)atof(token));
+ }else if(strcmp(token,"SHADE_RES")==0){
+ ALGetTwoInt(&sx,&sy);
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALFilRegion(fills,relnum[sx],sy,relnum[sx],sy,Yindx,(float)atof(token));
+ }else if(strcmp(token,"COLOUR_REGION")==0 || strcmp(token,"COLOR_REGION")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColRegion(s_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token));
+ }else if(strcmp(token,"COLOUR_TEXT_REGION")==0 || strcmp(token,"COLOR_TEXT_REGION")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColRRegion(text_colour,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token));
+ }else if(strcmp(token,"COLOUR_RES")==0 || strcmp(token,"COLOR_RES")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetTwoInt(&sx,&sy);
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColRRegion(s_colour,relnum[sx],sy,relnum[sx],sy,(unsigned char) atoi(token));
+ }else if(strcmp(token,"CCOLOUR_REGION")==0 || strcmp(token,"CCOLOR_REGION")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColRRegion(c_colour,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token));
+ }else if(strcmp(token,"COLOUR_LINE_REGION")==0 || strcmp(token,"COLOR_LINE_REGION")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColRegion(left_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token));
+ ALColRegion(right_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token));
+ ALColRegion(top_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token));
+ ALColRegion(bottom_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token));
+ }else if(strcmp(token,"CCOLOUR_RES")==0 || strcmp(token,"CCOLOR_RES")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetTwoInt(&sx,&sy);
+ token = strtok(NULL,TOKENS);
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax);
+ ALColRRegion(c_colour,relnum[sx],sy,relnum[sx],sy,(unsigned char) atoi(token));
+ }else if(strcmp(token,"MASK")==0){
+ /* mask commands */
+ token = strtok(NULL,TOKENS);
+ if(strcmp(token,"SETUP")==0){
+ if(mask == NULL){
+ mask = uchararr(bloc[1].slen+1,nseq+1);
+ GJUCinit(mask,bloc[1].slen+1,nseq+1,0);
+ }else{
+ GJUCinit(mask,bloc[1].slen+1,nseq+1,0);
+ }
+ }else if(strcmp(token,"RESET")==0){
+ GJUCinit(mask,bloc[1].slen+1,nseq+1,0);
+ }else if(strcmp(token,"LEGAL")==0){
+ /* get the legal character list */
+ token = strtok(NULL,TOKENS);
+ if(legal != NULL) GJfree(legal);
+ legal = GJstrdup(token);
+ if(!silent)fprintf(stderr,"legal:%s\n",legal);
+ }else if(strcmp(token,"ILLEGAL")==0){
+ /* get the illegal character list */
+ token = strtok(NULL,TOKENS);
+ if(illegal != NULL) GJfree(illegal);
+ illegal = GJstrdup(token);
+ if(!silent)fprintf(stderr,"illegal:%s:\n",illegal);
+ }else if(strcmp(token,"ID")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ id_cut = atoi(token);
+ if(id_cut < (int) ( ( ((float) (ey-sy+1) / 2.0) + 0.6))){
+ error("ID cutoff must be > half the number of sequences",1);
+ }
+ ALid_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,id_cut,legal,illegal);
+ }else if(strcmp(token,"AGREE")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token= strtok(NULL,TOKENS);
+ ALagree_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,atoi(token));
+ }else if(strcmp(token,"FRE")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALfre_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,legal,illegal);
+ }else if(strcmp(token,"NOT")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALnot_mask(mask,relnum[sx],sy,relnum[ex],ey);
+ }else if(strcmp(token,"SUB")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token=strtok(NULL,TOKENS);
+ ALsub_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,token[0]);
+ }else if(strcmp(token,"REGION")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALMask(mask,relnum[sx],sy,relnum[ex],ey);
+ }else if(strcmp(token,"CONSERVATION")==0){
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token=strtok(NULL,TOKENS);
+ conscut = atoi(token);
+ if(!silent)fprintf(std_err,"Conservation Cutoff:%d\n",conscut);
+ ALConsMask(mask,relnum[sx],sy,relnum[ex],ey,consval,conscut);
+ }else if(strcmp(token,"BOX")==0){
+ /* box the masked residues - ie surround them*/
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ ALSurroundMask(mask,bloc,lines,relnum[sx],sy,relnum[ex],ey,nseq);
+ }else if(strcmp(token,"SHADE")==0){
+ /* shade the masked residues - */
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALShadeMask(mask,fills,relnum[sx],sy,relnum[ex],ey,Yindx,(float)atof(token));
+ }else if(strcmp(token,"FONT")==0){
+ /* set the font of the masked residues - */
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALFontMask(mask,fonts,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token));
+ }else if(strcmp(token,"INVERSE")==0){
+ /* invert the masked residues - */
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALInverseMask(mask,inverse,relnum[sx],sy,relnum[ex],ey);
+ }else if(strcmp(token,"SCOL")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALSColMask(mask,s_colour,relnum[sx],sy,relnum[ex],ey,nseq,(unsigned char) atoi(token));
+ }else if(strcmp(token,"CCOL")==0){
+ if(!use_colour)error("You must use the DEFINE_COLOUR command first",1);
+ ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ ALColourMask(mask,c_colour,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token));
+ }else{
+ error("Unrecognised MASK qualifier\n",1);
+ }
+ }else if(strcmp(token,"TEXT")==0){
+ token=strtok(NULL,TOKENS);
+ sx = atoi(token);
+ token=strtok(NULL,"\"");
+ sy = atoi(token);
+ token=strtok(NULL,"\"");
+ ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax);
+ texts[relnum[sx]][sy] = (char *) malloc(sizeof(char)*(strlen(token)+1));
+ texts[relnum[sx]][sy] = (char *) strcpy(texts[relnum[sx]][sy],token);
+ }else if(strcmp(token,"NUMBERS")==0){
+ error("Numbers not implemented",1);
+ }else if(strcmp(token,"ID_FONT")==0){
+ token = strtok(NULL,TOKENS);
+ if(strcmp(token,"ALL")==0){
+ token = strtok(NULL,TOKENS);
+ for(i=1;i<nseq+1;++i)idfonts[i]= (unsigned char) atoi(token);
+ }else{
+ sx = atoi(token);
+ token = strtok(NULL,TOKENS);
+ ALCheckLegalRange(xmin,ymin,xmax,sx,xmin,ymin,xmax,ymax);
+ idfonts[sx] = atoi(token);
+ }
+ }else if(strcmp(token,"ID_COLOUR")==0){
+ token = strtok(NULL,TOKENS);
+ if(strcmp(token,"ALL")==0){
+ token = strtok(NULL,TOKENS);
+ for(i=1;i<nseq+1;++i)id_colour[i]= (unsigned char) atoi(token);
+ }else{
+ sx = atoi(token);
+ ALCheckLegalRange(xmin,ymin,xmax,sx,xmin,ymin,xmax,ymax);
+ token = strtok(NULL,TOKENS);
+ id_colour[sx] = atoi(token);
+ }
+ }else if(strcmp(token,"HELIX")==0){
+ ALGetThreeInt(&sx,&sy,&ex);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax);
+ ALMakeHelixText(relnum[sx],sy,relnum[ex],texts);
+ }else if(strcmp(token,"STRAND")==0){
+ ALGetThreeInt(&sx,&sy,&ex);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax);
+ ALMakeStrandText(relnum[sx],sy,relnum[ex],texts);
+ }else if(strcmp(token,"COIL")==0){
+ ALGetThreeInt(&sx,&sy,&ex);
+ ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax);
+ ALMakeCoilText(relnum[sx],sy,relnum[ex],texts);
+ }else if(strcmp(token,"CALCONS")==0){
+ /* calculate conservation for the range */
+ ALGetFourInt(&sx,&sy,&ex,&ey);
+ ALCheckLegalRange(relnum[sx],relnum[sy],relnum[ex],relnum[ey],xmin,ymin,xmax,ymax);
+ consval = ALCalCons(bloc,relnum[sx],relnum[sy],relnum[ex],relnum[ey],
+ consval,bloc[1].slen);
+/* fprintf(std_err,"Conservation Values\n"); */
+/* for(k=1;k<bloc[1].slen-1;++k){*/
+/* fprintf(std_err,"%d:%d\n",k,consval[k]);*/
+/* }*/
+ }else if(strcmp(token,"RELATIVE_TO")==0){
+ token = strtok(NULL,TOKENS);
+ relative_seq = atoi(token);
+ fprintf(stderr,"RELATIVE SEQ %d\n",relative_seq);
+ fprintf(stderr,"bloc[relative_seq].slen+1] %d\n",
+ bloc[relative_seq].slen+1);
+ token = strtok(NULL,TOKENS);
+ if(token != NULL){
+ /* there is a first residue definition */
+ firstres = atoi(token);
+ fprintf(stderr,"First position is number: %d\n",firstres);
+ }else{
+ firstres = 1;
+ }
+ if(relative_seq != 0){
+ k= firstres - 1;
+ for(i=1;i<bloc[1].slen+1;++i){
+ /* for now assume all gaps are blanks */
+ if(bloc[relative_seq].seq[i] != ' '){
+ ++k;
+ relnum[k] = i;
+ }
+ }
+ /* increase the max allowed X value to the max of xmax and k */
+ /* leave the minimum value at 1 since otherwise we will not be able to mix
+ absolute and relative numbers without the Check routine skwawking */
+ if(k > xmax ) xmax = k;
+ }else{
+ for(i=1;i<bloc[1].slen+1;++i){
+ relnum[i] = i;
+ }
+ }
+ }else{
+ fprintf(std_err,"%s",buff);
+ error("Unrecognised Step 2 command\n",1);
+ }
+ }
+ if(!silent){
+ ALReportInfo(nseq,std_err);
+ }
+ ALCheckSinglePage(nseq,bloc[1].slen,silent);
+
+ start_aa = 1;
+ totalpage = npage * Csheet;
+
+ /* get how many blocks of sequence we can fit per page */
+ nspage = Cheight/(nseq+CtopSpace+Vspacing);
+ if(nspage < 1){
+ nspage = 1;
+ }
+ nbp = 0;
+
+ if(!silent)fprintf(std_err,"Will write a maximum of %d blocks of sequence per page\n",nspage);
+ if(!silent)fprintf(std_err,"Background region: %d %d %d %d\n",
+ background_region[0],
+ background_region[1],
+ background_region[2],
+ background_region[3]);
+ if(!silent && singlepage)fprintf(std_err,"Bounding box: %d %d %d %d\n",
+ bounding_box[0],
+ bounding_box[1],
+ bounding_box[2],
+ bounding_box[3]);
+
+ /* MARKER */
+
+ if((Cwidth - Cidwidth > 0)){
+ if(!silent)fprintf(std_err,"Saving (%d) Pages to file\n",totalpage/nspage);
+ }else{
+ fprintf(std_err,"This pointsize is too large to print even a single character on the page\n");
+ fprintf(std_err,"Try a smaller pointsize - or\n");
+ fprintf(std_err,"Try reducing the Identifier width\n");
+ fprintf(std_err,"Increase the paper size\n");
+ fprintf(std_err,"You could do some PostScrip-ery to print partial characters\n");
+ fprintf(std_err,"After creating an alignment on a single page - see the CookBook\n");
+ exit(1);
+ }
+ lastY = 0;
+ nbpage = 0;
+ k = 0;
+ start_seq = 1;
+ while(k < totalpage){
+ start_aa = 1;
+ Ytemp = start_seq+Cheight-CtopSpace - 1;
+ if(Ytemp > nseq) Ytemp = nseq;
+ for(i=0;i<npage;++i){
+
+ if(nspage > 1){ /* more than one block per page */
+/* fprintf(stderr,"nbp nspage %d %d\n",nbp,nspage);*/
+ if(k>0 && nbp == 0){ /* if not the first page, then setup page*/
+ PSSetupPage(orientation,nbpage+1,outf);
+ if(background_colour != 99){
+ ALColourBackground(outf,background_colour,background_region);
+ }
+ }
+ if(nbp>0){
+ /* if this is not the first block on the page, then */
+ /* translate the block of alignment back to origin */
+ fprintf(outf,"%d %d translate\n",0,-lastY);
+ }
+ /* get new position for drawing and translate to this posn*/
+ lastY = Yspace*( Cheight-(nseq+CtopSpace)*(nbp+1) - (nbp*Vspacing) );
+ fprintf(outf,"%d %d translate\n",0,lastY);
+ }else{
+ if(k>0)PSSetupPage(orientation,k+1,outf);
+ if(background_colour != 99){
+ ALColourBackground(outf,background_colour,background_region);
+ }
+ }
+ if(id_only_on_first == 1 && nbp > 0 ){
+ /* save the Cseqwidth, then reset it to Cwidth - also set Cidwidth to 0*/
+ tempCseqwidth = Cseqwidth;
+ tempCidwidth = Cidwidth;
+ Cseqwidth = Cwidth;
+ Cidwidth = 0;
+ }
+
+ Xtemp = start_aa+Cseqwidth-1;
+ if(Xtemp > bloc[1].slen) Xtemp = bloc[1].slen;
+
+ fprintf(outf,"2 setlinecap\n");
+ fprintf(outf," %.2f setlinewidth\n",LineWidthFactor*pointsize);
+
+ ALDrawFills(fills,use_colour,s_colour,start_aa,nseq-Ytemp+1,Xtemp,nseq-start_seq+1,Cidwidth,CseqVoffset,outf);
+ ALDrawLines(lines,use_colour,
+ left_line_colour,
+ right_line_colour,
+ top_line_colour,
+ bottom_line_colour,
+ start_aa,nseq-Ytemp+1,Xtemp,nseq-start_seq+1,Cidwidth,CseqVoffset,outf);
+ if(!(id_only_on_first == 1 && (nbp > 0 || k > 0))){
+ /* don't output ID string if this is not the first block on the first page*/
+ ALOutId(bloc,use_colour,
+ id_colour,idfonts,start_seq,Ytemp,CseqVoffset,number_seqs,outf);
+ }
+ ALOutSeq(bloc,fonts,texts,inverse,use_colour,c_colour,
+ text_colour,background_colour,start_seq,Ytemp,start_aa,CseqVoffset,outf);
+
+ if(res_numbers){
+ if(use_colour)PSSetColour(number_colour,outf);
+ if(do_ticks){
+ ALOutTicks(ticks,start_aa,Cidwidth,Ytemp-start_seq+1,bloc[1].slen,outf);
+ ALOutNumbers(numbers,start_aa,Cidwidth,Ytemp-start_seq+2,bloc[1].slen,outf);
+ }else{
+ ALOutNumbers(numbers,start_aa,Cidwidth,Ytemp-start_seq+1,bloc[1].slen,outf);
+ }
+ }
+ start_aa += Cseqwidth;
+
+ if(nspage > 1){
+ ++nbp;
+ if(nbp == nspage || k == totalpage-1){ /* put showpage command for page or last page */
+ PSShowPage(outf);
+ nbp = 0;
+ ++nbpage;
+ }
+ }else{
+ PSShowPage(outf);
+ }
+ ++k;
+ }
+ start_seq = Ytemp + 1;
+ if(id_only_on_first == 1 && nbp > 0){
+ /* reset the values - not strictly necessary */
+ Cseqwidth = tempCseqwidth;
+ Cidwidth = tempCidwidth;
+ }
+ }
+ return 1;
+}
+
+void ALShadeChars(struct seqdat *bloc,
+signed char **fill,
+int start_x,int start_y,int end_x,int end_y,
+int maxY,
+char *charlist,
+float grey)
+
+/* shade residues in charlist by grey value */
+
+{
+ int i,j,jpos;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y,jpos=(maxY-start_y+1);j<end_y+1;++j,--jpos){
+ if(strchr(charlist,bloc[j].seq[i])!=NULL){
+ ALFilRes(fill,i,jpos,grey);
+ }
+ }
+ }
+}
+void ALSColChars(struct seqdat *bloc,unsigned char **colour,int start_x,
+int start_y,int end_x,int end_y,int maxY,char *charlist,unsigned char colnum)
+
+/* colour residue backgrounds in charlist by colnum value */
+
+{
+ int i,j,jpos;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y,jpos=(maxY-start_y+1);j<end_y+1;++j,--jpos){
+ if(strchr(charlist,bloc[j].seq[i])!=NULL){
+ ALColRes(colour,i,jpos,colnum);
+ }
+ }
+ }
+}
+
+void ALSColMask(unsigned char **mask,unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,int maxY,
+ unsigned char colnum)
+
+/* colour residue backgrounds in mask by colnum value */
+
+{
+ int i,j,jpos;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y,jpos=(maxY-start_y+1);j<end_y+1;++j,--jpos){
+ if(mask[i][j]==1){
+ ALColRes(colour,i,jpos,colnum);
+ }
+ }
+ }
+}
+void ALInverseChars(struct seqdat *bloc,unsigned char **inverse,
+ int start_x,int start_y,int end_x,int end_y,char *charlist)
+
+/* Invert the characters within the range */
+/* 18/Jan/93: Allow inverted chars to be reverted */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(strchr(charlist,bloc[j].seq[i])!=NULL){
+ if(inverse[i][j] == 1){
+ inverse[i][j] = (unsigned char) 0;
+ }else{
+ inverse[i][j] = (unsigned char ) 1;
+ }
+ }
+ }
+ }
+}
+void ALInverseMask(unsigned char **mask,unsigned char **inverse,
+ int start_x,int start_y,int end_x,int end_y)
+/* Invert the characters within the range of the mask*/
+/* 18/Jan/93: Allow inverted chars to be reverted */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(mask[i][j]){
+ if(inverse[i][j] == 1){
+ inverse[i][j] = (unsigned char) 0;
+ }else{
+ inverse[i][j] = (unsigned char ) 1;
+ }
+ }
+ }
+ }
+}
+
+void ALFontChars(struct seqdat *bloc,unsigned char **fonts,
+ int start_x,int start_y,int end_x,int end_y,char *charlist,unsigned char fontnum)
+
+/* set the characters within the range to the fontnum */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(strchr(charlist,bloc[j].seq[i])!=NULL){
+ fonts[i][j] = fontnum;
+ }
+ }
+ }
+}
+void ALFontRegion(unsigned char **fonts,
+ int start_x,int start_y,int end_x,int end_y,
+ unsigned char fontnum)
+/* set the characters within the range to the fontnum */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ fonts[i][j] = fontnum;
+ }
+ }
+}
+void ALFontMask(unsigned char **mask,unsigned char **fonts,
+ int start_x,int start_y,int end_x,int end_y,
+ unsigned char fontnum)
+
+/* set the characters within the range to the fontnum */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(mask[i][j]==1){
+ fonts[i][j] = fontnum;
+ }
+ }
+ }
+}
+
+void ALColourChars(struct seqdat *bloc,unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,
+ char *charlist,unsigned char colournum)
+
+/* set the characters within the range to the colour defined by colournum */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(strchr(charlist,bloc[j].seq[i])!=NULL){
+ colour[i][j] = colournum;
+ }
+ }
+ }
+}
+void ALColourRegion(unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,
+ unsigned char colournum)
+
+/* set the characters within the range to the colournum */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ colour[i][j] = colournum;
+ }
+ }
+}
+
+void ALColourMask(unsigned char **mask,unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,
+ unsigned char colournum)
+
+/* set the characters within the range to the colournum */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(mask[i][j] == 1){
+ colour[i][j] = colournum;
+ }
+ }
+ }
+}
+
+
+void ALSubChars(struct seqdat *bloc,
+ int start_x,int start_y,int end_x,int end_y,
+ char oldchar,char newchar)
+
+/* substitute oldchar for newchar at each position in bloc */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(bloc[j].seq[i] == oldchar) bloc[j].seq[i] = newchar;
+ }
+ }
+}
+
+
+void ALSurroundChars(struct seqdat *bloc,unsigned char **lines,
+ int start_x,int start_y,int end_x,int end_y,
+ int maxY,char *charlist)
+
+/*
+within the range of start_x etc. Draw lines such that every character that
+is present within charlist is surrounded by a box.
+*/
+{
+ int i,j,jpos;
+ char above,below,left,right;
+
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y,jpos=(maxY-start_y+1);j<end_y+1;++j,--jpos){
+ if(strchr(charlist,bloc[j].seq[i]) != NULL){
+/* fprintf(std_err,"%d %d %c %c\n",i,j,bloc[j].seq[i]*/
+ if(j == end_y){
+ below = '!';
+ }else{
+ below = bloc[j+1].seq[i];
+ }
+ if(j == start_y){
+ above = '!';
+ }else{
+ above = bloc[j-1].seq[i];
+ }
+ if(i == start_x){
+ left = '!';
+ }else{
+ left = bloc[j].seq[i-1];
+ }
+ if(i == end_x){
+ right = '!';
+ }else{
+ right = bloc[j].seq[i+1];
+ }
+
+ if(strchr(charlist,above)== NULL){
+ ALLineRes(lines,i,jpos,LINE_TOP);
+ }
+ if(strchr(charlist,below)== NULL){
+ ALLineRes(lines,i,jpos,LINE_BOTTOM);
+ }
+ if(strchr(charlist,left)==NULL){
+ ALLineRes(lines,i,jpos,LINE_LEFT);
+ }
+ if(strchr(charlist,right)==NULL){
+ ALLineRes(lines,i,jpos,LINE_RIGHT);
+ }
+ }
+ }
+ }
+}
+void ALSurroundMask(unsigned char **mask,struct seqdat *bloc,unsigned char **lines,
+ int start_x,int start_y,int end_x,int end_y,
+ int maxY)
+/*
+within the range of start_x etc. Draw lines such that every
+masked position is isolated from every non-masked position
+*/
+{
+ int i,j,jpos;
+ char above,below,left,right;
+
+/* fprintf(stdout,"ALSurroundMask: %d %d %d %d\n",start_x,start_y,end_x,end_y);*/
+
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y,jpos=(maxY-start_y+1);j<end_y+1;++j,--jpos){
+ if(mask[i][j] == 1){
+/* fprintf(std_err,"%d %d %c %c\n",i,j,bloc[j].seq[i]*/
+ if(j == end_y){
+ below = 0;
+ }else{
+ below = mask[i][j+1];
+ }
+ if(j == start_y){
+ above = 0;
+ }else{
+ above = mask[i][j-1];
+ }
+ if(i == start_x){
+ left = 0;
+ }else{
+ left = mask[i-1][j];
+ }
+ if(i == end_x){
+ right = 0;
+ }else{
+ right = mask[i+1][j];
+ }
+ /*
+ fprintf(stdout,"IN ALSurroundMask\n");
+ fprintf(stdout,"%d %d (%d %d %d %d)\n",i,j,above,below,left,right);
+ */
+
+ if(mask[i][j] != above){
+ ALLineRes(lines,i,jpos,LINE_TOP);
+ }
+ if(mask[i][j] != below){
+ ALLineRes(lines,i,jpos,LINE_BOTTOM);
+ }
+ if(mask[i][j] != left){
+ ALLineRes(lines,i,jpos,LINE_LEFT);
+ }
+ if(mask[i][j] != right){
+ ALLineRes(lines,i,jpos,LINE_RIGHT);
+ }
+ }
+ }
+ }
+}
+
+void ALFilRegion(signed char **fill,
+ int start_x,int start_y,int end_x,int end_y,
+ int *Yindx,float grey)
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ ALFilRes(fill,i,Yindx[j],grey);
+ }
+ }
+}
+
+void ALShadeMask(unsigned char **mask,signed char **fill,
+ int start_x,int start_y,int end_x,int end_y,int *Yindx,
+ float grey)
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ if(mask[i][j]==1){
+ ALFilRes(fill,i,Yindx[j],grey);
+ }
+ }
+ }
+}
+
+
+void ALColRegion(unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,
+ int *Yindx,unsigned char colnum)
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ ALColRes(colour,i,Yindx[j],colnum);
+ }
+ }
+}
+
+void ALColRRegion(unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,
+ unsigned char colnum)
+/* version of ALColRegion that is indexed by characters rather than position */
+/* 18/1/1995 */
+
+{
+ int i,j;
+ for(i=start_x;i<end_x+1;++i){
+ for(j=start_y;j<end_y+1;++j){
+ colour[i][j] = colnum;
+ }
+ }
+}
+
+void ALBoxRegion(unsigned char **lines,
+ int start_x,int start_y,int end_x,int end_y,
+ int *Yindx)
+{
+ int i;
+ for(i=start_x;i<end_x+1;++i){
+ ALLineRes(lines,i,Yindx[end_y],LINE_BOTTOM);
+ ALLineRes(lines,i,Yindx[start_y],LINE_TOP);
+ }
+ for(i=start_y;i<end_y+1;++i){
+ ALLineRes(lines,start_x,Yindx[i],LINE_LEFT);
+ ALLineRes(lines,end_x,Yindx[i],LINE_RIGHT);
+ }
+}
+void ALDoLine(unsigned char **lines,
+ int start_x,int start_y,int end,
+ int type,int *Yindx)
+{
+ int i;
+ if(type == LINE_TOP || type == LINE_BOTTOM){
+ for(i=start_x;i<end+1;++i){
+ ALLineRes(lines,i,Yindx[start_y],type);
+ }
+ }else{
+ for(i=start_y;i<end+1;++i){
+ ALLineRes(lines,start_x,Yindx[i],type);
+
+
+ }
+ }
+}
+
+void ALDrawFills(signed char **fill,int use_colour,unsigned char **colour,
+ int start_x,int start_y,int end_x,int end_y,int XOffset,int YOffset,FILE *outf)
+
+/* for values of fill >= 0 draw box and fill in with grey value */
+/* 6/12/92: add colour option */
+{
+ int i,j;
+ extern int precis;
+
+ XOffset *= Xspace;
+ YOffset *= Yspace;
+
+ for(i=end_x;i>(start_x-1);--i){
+ for(j=end_y;j>(start_y-1);--j){
+ if((int) fill[i][j] >= 0 || use_colour){
+
+/*
+Revise to use ML function 27th Oct 1992
+ fprintf(outf," newpath\n");
+ fprintf(outf,"%.2f %.2f moveto \n",(float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset);
+ fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset);
+ fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset);
+ fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset);
+ fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset);
+ fprintf(outf,"closepath %.2f setgray fill 0 setgray \n",(float)fill[i][j]/(float)precis);
+*/
+ fprintf(outf," newpath\n");
+ fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f ML\n",
+ (float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset,
+ (float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset,
+ (float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset,
+ (float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset,
+ (float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset);
+ if(use_colour && fill[i][j] < 0){
+ /* 6/1/1995 - only set colour if grey is not set */
+ /* set to colour number */
+ fprintf(outf,"closepath C%-d fill \n",colour[i][j]);
+ }else{
+ fprintf(outf,"closepath %.2f setgray fill 0 setgray \n",(float)fill[i][j]/(float)precis);
+ }
+ }
+ }
+ }
+}
+
+void ALFilRes(signed char **fill,int i,int j,float grey)
+
+{
+ extern int precis;
+/* ALBoxRes(lines,i,j);
+ fill[i+Cidwidth][j+CseqVoffset] = grey * precis;
+*/
+ fill[i][j] = grey * precis;
+}
+
+void ALColRes(unsigned char **colour,int i,int j,int colnum)
+
+{
+ colour[i][j] = colnum;
+}
+
+
+void ALBoxRes(unsigned char **lines,int i,int j)
+
+{
+ ALLineRes(lines,i,j,LINE_TOP);
+ ALLineRes(lines,i,j,LINE_BOTTOM);
+ ALLineRes(lines,i,j,LINE_LEFT);
+ ALLineRes(lines,i,j,LINE_RIGHT);
+}
+
+
+void ALLineRes(
+unsigned char **lines,
+int i,int j,
+unsigned char value)
+{
+/*
+ i = i + Cidwidth;
+ j = j + CseqVoffset;
+*/
+ ALLineChar(lines,i,j,value);
+}
+
+void ALLineChar(unsigned char **lines,int i,int j,unsigned char value)
+
+{
+ lines[i][j] = lines[i][j] | value;
+}
+
+void ALDrawLines(unsigned char **lines,int use_colour,
+ unsigned char **left_line_colour,
+ unsigned char **right_line_colour,
+ unsigned char **top_line_colour,
+ unsigned char **bottom_line_colour,
+ int start_x,int start_y,int end_x,int end_y,
+ int XOffset,int YOffset,FILE *outf)
+
+/* draw the lines that are indicated by the lines array in the range of the
+array indicated by start_x etc
+*/
+{
+ int i,j;
+ XOffset *= Xspace;
+ YOffset *= Yspace;
+
+/*
+ if(use_colour){
+ fprintf(outf,"C100\n");
+ }
+*/
+
+ for(i=end_x;i>(start_x-1);--i){
+ for(j=end_y;j>(start_y-1);--j){
+ if(lines[i][j] != 0){
+ if((LINE_LEFT & lines[i][j]) == LINE_LEFT){
+ PSSetColour(left_line_colour[i][j],outf);
+ PSline((float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset,
+ (float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset,outf);
+ }
+ if((LINE_RIGHT & lines[i][j]) == LINE_RIGHT){
+ PSSetColour(right_line_colour[i][j],outf);
+ PSline((float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset,
+ (float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset,outf);
+ }
+ if((LINE_TOP & lines[i][j]) == LINE_TOP){
+ PSSetColour(top_line_colour[i][j],outf);
+ PSline((float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset,
+ (float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+1)*Yspace-Yshift+YOffset,outf);
+ }
+ if((LINE_BOTTOM & lines[i][j]) == LINE_BOTTOM){
+ PSSetColour(bottom_line_colour[i][j],outf);
+ PSline((float)(i-start_x)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset,
+ (float)(i-start_x+1)*Xspace-Xshift+XOffset,
+ (float)(j-start_y+0)*Yspace-Yshift+YOffset,outf);
+ }
+ }
+ }
+ }
+ /*
+ if(use_colour){
+ fprintf(outf,"C99\n");
+ }
+ */
+}
+
+void PSline(float x1,float y1,float x2,float y2,FILE *outf)
+
+{
+ fprintf(outf,"%.2f %.2f %.2f %.2f L\n ",x1,y1,x2,y2);
+}
+
+
+
+void ALSimpleBox(int x1,int y1,int x2,int y2,float pfactor, /* multiply pointsize by this to get linewidth */
+ FILE *outf)
+
+/* draw a box to include character coordinates x1,y1 to x2,y2 */
+/* x1 < x2 y1< y2 */
+{
+ float lshift = Xspace/6;
+ float rshift = Xspace - lshift;
+
+ x1 *= Xspace;
+ y1 *= Yspace;
+ x2 *= Xspace;
+ y2 *= Yspace;
+
+ x1 -= lshift;
+ x2 += rshift;
+ y1 -= lshift;
+
+ PSDrawBox((float)x1,(float)y1,(float)x2,(float)y2,pfactor,outf);
+}
+
+void PSDrawBox(float x1,float y1,float x2,float y2,float pfactor,FILE *out)
+
+{
+ fprintf(out,"%f setlinewidth\n ",pointsize*pfactor);
+ fprintf(out,"%f %f moveto %f %f lineto %f %f lineto %f %f lineto %f %f lineto closepath stroke\n",
+ x1,y1,x2,y1,x2,y2,x1,y2,x1,y1);
+}
+
+void ALOutTicks(char *array,int start_aa,int xpos,int ypos,int len,FILE *out)
+
+{
+ int right;
+ int j;
+
+ xpos *= Xspace;
+ ypos *= Yspace;
+
+ right = start_aa + Cseqwidth;
+ if(right > len)right = len;
+ for(j=start_aa;j<right;j++){
+ if(array[j] != '\0'){
+ PSPutChar(xpos,ypos,array[j],out);
+ }
+ xpos += Xspace;
+ }
+}
+
+void ALOutNumbers(char **array,int start_aa,int xpos,int ypos,int len,FILE *out)
+
+{
+ int right;
+ int j;
+
+ xpos *= Xspace;
+ ypos *= Yspace;
+
+ right = start_aa + Cseqwidth;
+ if(right > len)right = len;
+ for(j=start_aa;j<right;j++){
+ if(array[j][0] != '\0'){
+ PSPutText(xpos,ypos,array[j],out);
+ }
+ xpos += Xspace;
+ }
+}
+
+char *ALCreateTicks(int start, int interval, int len)
+
+/* create ticks at the desired interval starting with residue start */
+{
+ int i;
+ char *temp;
+ temp = (char *) malloc(sizeof(char) * len);
+ /* first set all ticks to '\0' */
+ for(i=0;i<len;++i){
+ temp[i]='\0';
+ }
+ for(i=0;i<len;i+=interval){
+ temp[i] = '|';
+ }
+ temp[0]='\0';
+ temp[1]='|';
+ return temp;
+}
+
+
+
+char **ALCreateNumbers(int start,int interval,int nres,int nwidth)
+
+/* create character array of numbers from start to nres at interval*/
+{
+ int i;
+ char **temp;
+ temp = (char **) malloc(nres*sizeof(char *));
+ for(i=0;i<nres;++i){
+ temp[i] = (char *) malloc(sizeof(char) *nwidth);
+ temp[i][0]='\0';
+ }
+ for(i=start;i<nres;i+=interval){
+ if(i==0){
+ sprintf(temp[i+1],"%-d",i+1);
+ }else{
+ sprintf(temp[i],"%-d",i);
+ }
+ }
+
+ return temp;
+}
+
+char **ALCreateNum2(
+int nstart, /* starting number */
+int ninterval, /* interval for numbers */
+int nend, /* end of number range */
+int nres, /* total length of sequence */
+int xstart, /* starting location for writing numbers */
+int nwidth) /* width of the number string */
+
+/* create character array of numbers from start to nres at interval*/
+{
+ int i,xp;
+ char **temp;
+ temp = (char **) malloc(nres*sizeof(char *));
+ for(i=0;i<nres;++i){
+ temp[i] = (char *) malloc(sizeof(char) *nwidth);
+ temp[i][0]='\0';
+ }
+ xp = xstart;
+ for(i=nstart;i<nend;i+=ninterval){
+ if(i==0){
+ sprintf(temp[xp+1],"%-d",i+1);
+ }else{
+ sprintf(temp[xp],"%-d",i);
+ }
+ xp += ninterval;
+ }
+
+ return temp;
+}
+
+void ALReportInfo(int nseq,FILE *out)
+
+{
+ fprintf(out,"Number of Sequences: %d\n",nseq);
+ fprintf(out,"Total Vertical Space per page: %d\n",Cheight);
+}
+
+void ALReserveSpace(int location,int nspace)
+
+{
+ extern int TVspace,CseqVoffset;
+
+ switch(location){
+ case TOP:
+ TVspace += nspace;
+ CtopSpace +=nspace;
+ break;
+ case BOTTOM:
+ TVspace += nspace;
+ CseqVoffset += nspace;
+ CbottomSpace += nspace;
+ break;
+ }
+}
+
+void PSSetupPage(int orientation,int npage,FILE *outf)
+
+{
+ fprintf(outf,"%%%%Page: ? %d\n",npage);
+ PSSetOrientation(orientation,outf);
+ PSSetFont(0,outf); /* set default font for document */
+}
+
+void ALOutSeq(struct seqdat *bloc,unsigned char **fonts,char ***texts,unsigned char **inverse,
+ int use_colour,
+ unsigned char **colour,
+ unsigned char **text_colour,
+ unsigned char background_colour,
+ int start_seq,int end_seq,int start_aa,int ypos,FILE *outf)
+
+{
+ extern int Xspace,Yspace;
+ extern int Cidwidth;
+
+ int i,j;
+ int xpos;
+ unsigned char current_col;
+
+ int right;
+
+ ypos *= Yspace;
+
+ current_col = background_colour;
+
+ right = start_aa + Cseqwidth;
+ if(right > bloc[1].slen-1)right = bloc[1].slen;
+
+ for(i=end_seq;i>(start_seq-1);--i){
+ xpos = Cidwidth * Xspace;
+ for(j=start_aa;j<right;++j){
+ if(texts[j][i] != NULL || bloc[i].seq[j] != ' '){
+ if(fonts[j][i]!=0)PSSetFont(fonts[j][i],outf);
+ if(inverse[j][i] != 0)PSSetGrey(1,outf);
+ if(use_colour && colour[j][i] != current_col){
+ PSSetColour(colour[j][i],outf);
+ current_col = colour[j][i];
+ }
+ if(bloc[i].seq[j] != ' '){
+ PSPutChar(xpos,ypos,bloc[i].seq[j],outf);
+ }
+ if(texts[j][i] != NULL){
+ if(text_colour[j][i] != current_col){
+ PSSetColour(text_colour[j][i],outf);
+ current_col = text_colour[j][i];
+ }
+ if(texts[j][i][0] == '@'){
+ PSPutSpecialText(xpos,ypos,Xspace,Yspace,texts[j][i],
+ text_colour[j][i],background_colour,0.9,outf);
+ }else{
+ PSPutText(xpos,ypos,texts[j][i],outf);
+ }
+ }
+ if(inverse[j][i] != 0)PSSetGrey(0,outf);
+ if(fonts[j][i]!=0)PSSetFont(0,outf);
+ }
+ xpos += Xspace;
+ }
+ ypos += Yspace;
+ }
+/*
+ if(use_colour){
+ PSSetColour(100,outf);
+ }
+*/
+}
+
+
+void ALOutId(struct seqdat *bloc,int use_colour,
+ unsigned char *colour,
+ unsigned char *fonts,
+ int start_seq,int end_seq,int ypos,int numbers,
+ FILE *outf)
+
+{
+ extern int MAXilen;
+ extern int Xspace,Yspace;
+ int i;
+ int xpos;
+ char *temp;
+
+ xpos = 0;
+ ypos *= Yspace;
+ temp = (char *) malloc(sizeof(char) * (MAXilen + 5));
+
+
+ for(i=end_seq;i>(start_seq-1);--i){
+ if(use_colour)PSSetColour(colour[i],outf);
+ if(fonts[i] != 0)PSSetFont(fonts[i],outf);
+ if(numbers){
+ sprintf(temp,"%4d:%s",i,bloc[i].id);
+ }else{
+ sprintf(temp,"%s",bloc[i].id);
+ }
+ PSPutText(xpos,ypos,temp,outf);
+ ypos += Yspace;
+ if(fonts[i] != 0)PSSetFont(0,outf);
+ }
+ free((char *) temp);
+
+}
+
+void PSPutText(int x,int y,char *text,FILE *outf)
+
+{
+ fprintf(outf," %d %d moveto (%s) show \n",x,y,text);
+}
+
+void PSPutChar(int x,int y,char c,FILE *outf)
+
+{
+ /* fprintf(outf," %d %d moveto (%c) show \n",x,y,c);*/
+ fprintf(outf,"(%c) %d %d P\n",c,x,y);
+}
+
+void PSShowPage(FILE *outf)
+
+{
+ fprintf(outf,"showpage\n");
+}
+
+void ALSetFont(char *font,int point,FILE *outf)
+
+{
+ fprintf(outf,"/%s findfont %d scalefont setfont\n",font,point);
+}
+
+void PSSetFont(unsigned char number,FILE *outf)
+
+{
+ fprintf(outf,"F%-d\n ",number);
+}
+
+void PSSetColour(unsigned char number,FILE *outf)
+
+{
+ fprintf(outf,"C%-d\n ",number);
+}
+
+void ALDefineFont(int number,char *font,float point,FILE *outf)
+
+/* define a PostScript function called /Fnumber which selects the
+font at point pointsize */
+{
+ fprintf(outf,"/F%-d {/%s findfont %.2f scalefont setfont } def\n",
+ number,font,point);
+}
+
+void ALDefineColour(int number,char *type,float red,float green,float blue,FILE *outf)
+
+/* define a PostScript function called /Cnumber which selects the
+colour defined by red,green, blue intensities
+
+new addition - if type is equal to "HSB" then define an hsb colour using red, green blue as
+the hue saturation and brightness.
+*/
+{
+ if(strcmp(type,"HSB")==0){
+ /* set an HSB colour */
+ fprintf(outf,"/C%-d {%f %f %f sethsbcolor } def\n", number,red,green,blue);
+ }else{
+ /* set an RGB colour */
+ fprintf(outf,"/C%-d {%f %f %f setrgbcolor } def\n", number,red,green,blue);
+ }
+}
+
+void ALCheckSinglePage(int nseq,int len,int silent)
+
+{
+ extern FILE *std_err;
+ Csheet = 1;
+
+
+ if(Cheight-CtopSpace < nseq){
+ if(!silent)fprintf(std_err,"Not enough height to print all sequences on a single page at this pointsize\n");
+ if(!silent)fprintf(std_err,"Pointsize: %.2f\n",pointsize);
+ if(!silent)fprintf(std_err,"Need a pointsize of < : %.2f\n",(float) height/(nseq+ CtopSpace));
+ Csheet = nseq/(Cheight-CtopSpace) + 1;
+ if(!silent)fprintf(std_err,"Will split alignment into %d segments\n",Csheet);
+ }
+
+ npage = len/(Cwidth - Cidwidth) + 1;
+}
+
+
+void ALSetPageLimits(void)
+/* Use the current width height and pointsize values to calculate:
+ Cwidth
+ Cheight
+ Xspace
+ Yspace
+*/
+{
+
+ Xspace = pointsize + pointsize * XspaceFactor;
+ Yspace = pointsize + pointsize * YspaceFactor;
+
+ Cwidth = width/Xspace;
+ Cheight = height/Yspace;
+
+ Cseqwidth = Cwidth - Cidwidth;
+
+ Xshift = Xspace * XshiftFactor;
+ Yshift = Yspace * YshiftFactor;
+}
+
+void PSSetOrientation(int orientation,FILE *outf)
+
+{
+ switch(orientation){
+ case LANDSCAPE:
+ PSLandscape(outf);
+ break;
+ case PORTRAIT:
+ PSPortrait(outf);
+ break;
+ default:
+ PSPortrait(outf);
+ }
+}
+
+void PSPreamble(FILE *out,int singlepage,int *b_box,int screensize)
+{
+ char *Alscript_Version = ALSCRIPT_VERSION;
+ fprintf(out,"%%!PS-Adobe-2.0\n");
+ fprintf(out,"%%%%Creator: %s\n",Alscript_Version);
+ fprintf(out,"%%%%Title: Alscript Output\n");
+ fprintf(out,"%% Barto,n G. J. (1993)\n");
+ fprintf(out,"%% ALSCRIPT - A Tool to format multiple sequence alignments\n");
+ fprintf(out,"%% Protein Engineering, 6, 37-40\n");
+ fprintf(out,"%%\n");
+ if(singlepage == 1){
+ fprintf(out,"%%%%BoundingBox: %d %d %d %d\n",
+ b_box[0],b_box[1],b_box[2],b_box[3]);
+ }
+ fprintf(out,"/P {moveto show} def\n");
+ fprintf(out,"/L {moveto lineto stroke} def\n");
+ fprintf(out,
+ "/ML {moveto lineto lineto lineto lineto} def\n");
+ fprintf(out,"2 setlinecap\n"); /* projecting line caps */
+ ALDefineColour(99,"RGB",1.0,1.0,1.0,out); /* white */
+ ALDefineColour(100,"RGB",0.0,0.0,0.0,out); /* black */
+ /* modify the screening - suggested by Michael D. Baron */
+ fprintf(out,"%d currentscreen 3 -1 roll pop setscreen\n",screensize);
+
+}
+
+void PSLandscape(FILE *out)
+
+{
+ width = MAXside;
+ height = MINside;
+
+ fprintf(out,"%d %d translate\n",xoff+height,yoff);
+ fprintf(out,"90 rotate\n");
+
+}
+
+void PSPortrait(FILE *out)
+
+{
+ width = MINside;
+ height = MAXside;
+ fprintf(out,"%d %d translate\n",xoff,yoff);
+}
+
+
+void echo(char *s)
+
+{
+ extern FILE *std_err;
+ fprintf(std_err,"%s",s);
+}
+
+void ALGetFourInt(int *sx,int *sy,int *ex,int *ey)
+
+{
+char *token;
+
+ token = strtok(NULL,TOKENS);
+ *sx = atoi(token);
+ token = strtok(NULL,TOKENS);
+ *sy = atoi(token);
+ token = strtok(NULL,TOKENS);
+ *ex = atoi(token);
+ token = strtok(NULL,TOKENS);
+ *ey = atoi(token);
+}
+void ALGetThreeInt(int *sy,int *ex,int *ey)
+
+{
+char *token;
+
+ token = strtok(NULL,TOKENS);
+ *sy = atoi(token);
+ token = strtok(NULL,TOKENS);
+ *ex = atoi(token);
+ token = strtok(NULL,TOKENS);
+ *ey = atoi(token);
+}
+void ALGetTwoInt(int *sy,int *ex)
+
+{
+char *token;
+
+ token = strtok(NULL,TOKENS);
+ *sy = atoi(token);
+ token = strtok(NULL,TOKENS);
+ *ex = atoi(token);
+}
+
+char ALChekSpace(const char *token)
+{
+ if(strcmp(token,"SPACE") == 0){
+ return ' ';
+ }else{
+ return token[0];
+ }
+}
+
+void PSSetGrey(float grey,FILE *outf)
+{
+ fprintf(outf," %.2f setgray\n",grey);
+}
+
+int save_pir(struct seqdat *bloc,int nseq,FILE *pirf)
+
+{
+ int i,j;
+ int count;
+
+
+ for(i=1;i<(nseq+1);++i){
+ fprintf(pirf,">P1;%s\n",bloc[i].id);
+ fprintf(pirf,"%s\n",bloc[i].title);
+ count=0;
+ for(j=1;j<bloc[i].slen;++j){
+ ++count;
+ /* convert spaces to dashes */
+ if(bloc[i].seq[j] == ' ')bloc[i].seq[j] = '.';
+ fputc(bloc[i].seq[j],pirf);
+ if(count==50){
+ count=0;
+ fprintf(pirf,"\n");
+ }
+ }
+ fprintf(pirf,"*\n");
+ }
+ return 1;
+}
+
+int save_msf(struct seqdat *bloc,int nseq,FILE *msff)
+
+{
+ int i,j,s,e;
+ int count;
+
+ fprintf(msff,"\njunk.msf MSF: %d Type: P January 01, 1776 12:00 Check: 7110 ..\n\n",bloc[1].slen-1);
+
+ for(i=1;i<(nseq+1);++i){
+ fprintf(msff,"Name: %20s Len: %7d Check: 0 Weight: 1.00\n",
+ bloc[i].id,bloc[1].slen-1);
+ }
+ fprintf(msff,"\n//\n\n\n");
+
+
+ s = 1;
+
+ while(s < bloc[1].slen){
+ e = s + 50;
+ if(e > bloc[1].slen) e = bloc[1].slen;
+
+ for(i=1;i<(nseq+1);++i){
+ fprintf(msff,"%10s ",bloc[i].id);
+ for(j=s;j<e;++j){
+ if(bloc[i].seq[j] == ' ')bloc[i].seq[j]='.';
+ fputc(bloc[i].seq[j],msff);
+ }
+ fprintf(msff,"\n");
+ }
+ s += 50;
+ fprintf(msff,"\n\n");
+ }
+ return 1;
+}
+
+
+void ALGetAllRange(int *sx, int *sy, int *ex, int *ey,int dex,int dey)
+/* checks the next token if it is ALL then return box around whole alignment
+ else return box according to the integers found
+*/
+{
+ char *token;
+
+ token = strtok(NULL,TOKENS);
+
+ if(strcmp(token,"ALL")==0){
+ *sx = 1;
+ *sy = 1;
+ *ex = dex;
+ *ey = dey;
+ }else{
+ *sx = atoi(token);
+ ALGetThreeInt(sy,ex,ey);
+ }
+ if(*ex > dex) *ex = dex;
+ if(*ey > dey) *ey = dey;
+}
+
+void ALid_mask(
+unsigned char **mask,
+struct seqdat *bloc,
+int sx,int sy,int ex,int ey,
+int id_cut,
+char *legal,
+char *illegal)
+
+{
+ int i,j,k,iseen;
+ char fchar;
+ char *seen; /* array of characters that are seen at this position */
+ int *freq; /* array of frequencies of the characters */
+ int nc; /* number of different character types at this position */
+ int ns;
+ int mc; /* maximum character present */
+ int imc; /* location of max character */
+
+ ns = ey - sy + 1;
+ nc = 0;
+
+ seen = (char *) GJmalloc(sizeof(char) *ns);
+ freq = (int *) GJmalloc(sizeof(int) *ns);
+
+ for(i=0;i<ns;++i){
+ freq[i] = 0;
+ seen[i] = '\0';
+ }
+
+/* fprintf(stdout,"In ALidmask\n");*/
+
+ for(i=sx;i<(ex+1);++i){
+ /* for each position get the list of characters seen and their frequencies */
+ for(j=sy;j<(ey+1);++j){
+ iseen = Ifound(bloc,i,j,seen,nc);
+ if(iseen >= 0){
+ ++freq[iseen];
+ }else{
+ seen[nc] = bloc[j].seq[i];
+ ++freq[nc];
+ ++nc;
+ }
+ }
+ /*
+ for(k=0;k<nc;++k)fprintf(stdout,"%c ",seen[k]);
+ for(k=0;k<nc;++k)fprintf(stdout,"%d ",freq[k]);
+ fprintf(stdout,"\n");
+ */
+ /* find the most frequent character that is legal and not illegal*/
+ mc = 0;
+ for(k=0;k<nc;++k){
+ if(legal != NULL && illegal != NULL){
+ if( strchr(legal,seen[k]) != NULL
+ && strchr(illegal,seen[k]) == NULL
+ && freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }else if(legal != NULL){
+ if( strchr(legal,seen[k]) != NULL
+ && freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }else if(illegal != NULL){
+ if( strchr(illegal,seen[k]) == NULL
+ && freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }else{
+ if(freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }
+ }
+ if(mc >= id_cut){
+ /* only select the character if it is >= id_cut */
+ fchar = seen[imc];
+/* fprintf(stdout,"At %d %c\n",i,fchar);*/
+ for(j=sy;j<(ey+1);++j){
+ if(bloc[j].seq[i] == fchar){
+ mask[i][j] = 1;
+/* fprintf(stdout,"%d %d \n",i,j);*/
+ }
+ }
+ }
+ for(j=0;j<ns;++j){
+ freq[j] = 0;
+ seen[j] = '\0';
+ }
+ nc = 0;
+ }
+ GJfree(seen);
+ GJfree(freq);
+}
+
+void ALfre_mask(
+unsigned char **mask,
+struct seqdat *bloc,
+int sx,int sy,int ex,int ey,
+char *legal,
+char *illegal)
+
+{
+ int i,j,k,iseen;
+ char fchar;
+ char *seen; /* array of characters that are seen at this position */
+ int *freq; /* array of frequencies of the characters */
+ int nc; /* number of different character types at this position */
+ int ns;
+ int mc; /* maximum character present */
+ int imc; /* location of max character */
+
+ ns = ey - sy + 1;
+ nc = 0;
+
+ seen = (char *) GJmalloc(sizeof(char) *ns);
+ freq = (int *) GJmalloc(sizeof(int) *ns);
+
+ for(i=0;i<ns;++i){
+ freq[i] = 0;
+ seen[i] = '\0';
+ }
+
+/* fprintf(stdout,"In ALidmask\n");*/
+
+ for(i=sx;i<(ex+1);++i){
+ /* for each position get the list of characters seen and their frequencies */
+ for(j=sy;j<(ey+1);++j){
+ iseen = Ifound(bloc,i,j,seen,nc);
+ if(iseen >= 0){
+ ++freq[iseen];
+ }else{
+ seen[nc] = bloc[j].seq[i];
+ ++freq[nc];
+ ++nc;
+ }
+ }
+/* for(k=0;k<nc;++k)fprintf(stdout,"%c ",seen[k]);
+ for(k=0;k<nc;++k)fprintf(stdout,"%d ",freq[k]);
+ fprintf(stdout,"\n");
+*/
+ /* find the most frequent character that is legal and not illegal*/
+ mc = 0;
+ for(k=0;k<nc;++k){
+ if(legal != NULL && illegal != NULL){
+ if( strchr(legal,seen[k]) != NULL
+ && strchr(illegal,seen[k]) == NULL
+ && freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }else if(legal != NULL){
+ if( strchr(legal,seen[k]) != NULL
+ && freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }else if(illegal != NULL){
+ if( strchr(illegal,seen[k]) == NULL
+ && freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }else{
+ if(freq[k] > mc){
+ mc = freq[k];
+ imc = k;
+ }
+ }
+ }
+ fchar = seen[imc];
+/* fprintf(stdout,"At %d %c\n",i,fchar);*/
+ for(j=sy;j<(ey+1);++j){
+ if(bloc[j].seq[i] == fchar){
+ mask[i][j] = 1;
+/* fprintf(stdout,"%d %d \n",i,j);*/
+ }
+ }
+
+ for(j=0;j<ns;++j){
+ freq[j] = 0;
+ seen[j] = '\0';
+ }
+ nc = 0;
+ }
+ GJfree(seen);
+ GJfree(freq);
+}
+int Ifound(struct seqdat *bloc,
+int i,int j,
+char *seen,
+int nc)
+{
+ int k;
+
+ if(nc > 0){
+ for(k=0;k<nc;++k){
+ if(bloc[j].seq[i] == seen[k]){
+ return k;
+ }
+ }
+ }
+ return -1;
+}
+void ALagree_mask(
+unsigned char **mask,
+struct seqdat *bloc,
+int sx,int sy,int ex,int ey,
+int ns)
+
+{
+ int i,j;
+
+ for(i=sx;i<(ex+1);++i){
+ /* for each position mask the characters that are identical to sequence ns */
+ for(j=sy;j<(ey+1);++j){
+ if(bloc[j].seq[i] == bloc[ns].seq[i]){
+ mask[i][j] = 1;
+ }
+ }
+ }
+}
+
+void ALnot_mask(
+unsigned char **mask,
+int sx,int sy,int ex,int ey)
+
+{
+ int i,j;
+
+ for(i=sx;i<(ex+1);++i){
+ /* NOT on each mask element ie 1 goes to 0, 0 to 1 */
+ for(j=sy;j<(ey+1);++j){
+ if(mask[i][j]==1){
+ mask[i][j]=0;
+ }else if(mask[i][j]==0){
+ mask[i][j]=1;
+ }
+ }
+ }
+}
+
+
+void ALsub_mask(unsigned char **mask,struct seqdat *bloc,
+ int sx,int sy,int ex,int ey,char cchar)
+
+{
+ int i,j;
+
+ for(i=sx;i<(ex+1);++i){
+ /* substitute all characters in the mask for cchar */
+ for(j=sy;j<(ey+1);++j){
+ if(mask[i][j]==1){
+ bloc[j].seq[i] = cchar;
+ }
+ }
+ }
+}
+
+void ALMask(unsigned char **mask,
+int sx,int sy,int ex,int ey)
+
+{
+ int i,j;
+
+ for(i=sx;i<(ex+1);++i){
+ /* substitute all characters in the mask for cchar */
+ for(j=sy;j<(ey+1);++j){
+ mask[i][j] = 1;
+ }
+ }
+}
+
+void ALConsMask(unsigned char **mask,
+int sx,int sy,int ex,int ey,int *consval,int conscut)
+
+{
+ int i,j;
+
+ for(i=sx;i<(ex+1);++i){
+ /* set mask at all positions that are >= conscut in the consval array */
+ for(j=sy;j<(ey+1);++j){
+ if(consval[i] >= conscut){
+ mask[i][j] = 1;
+ }
+ }
+ }
+}
+
+
+void ALDrawHorizLine(
+ float Xpos, /* bottom left hand corner of character posn */
+ float Ypos, /* */
+ float Xspace, /* horizontal width of character position */
+ float Yspace, /* vertical height of character position */
+ float width_fac,FILE *outf) /* proportion of vertical height that the line will fill */
+
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+
+ /* width_fac defines the width of the line in terms of the height of the characters */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f ML\n",
+ Xpos,ymid-hline_w,
+ xright,ymid-hline_w,
+ xright,ymid+hline_w,
+ Xpos,ymid+hline_w,
+ Xpos,ymid-hline_w,outf);
+}
+
+void ALDrawRightArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+
+ /* width_fac defines the width of the Arrow in terms of the height of the characters */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSmoveto(Xpos,Ypos,outf);
+ PSlineto(xright,ymid,outf);
+ PSlineto(Xpos,ytop,outf);
+ PSlineto(Xpos,Ypos,outf);
+
+}
+
+void ALDrawLeftArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+
+ /* width_fac defines the width of the Arrow in terms of the height of the characters */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSmoveto(xright,Ypos,outf);
+ PSlineto(xright,ytop,outf);
+ PSlineto(Xpos,ymid,outf);
+ PSlineto(xright,Ypos,outf);
+}
+
+
+void ALDrawUpArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSmoveto(Xpos,Ypos,outf);
+ PSlineto(xright,Ypos,outf);
+ PSlineto(xmid,ytop,outf);
+ PSlineto(Xpos,Ypos,outf);
+}
+
+void ALDrawDownArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSmoveto(Xpos,ytop,outf);
+ PSlineto(xright,ytop,outf);
+ PSlineto(xmid,Ypos,outf);
+ PSlineto(Xpos,ytop,outf);
+}
+
+/* Draw a small up arrow. mok 980917 */
+void ALDrawSmallUpArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float xmid;
+ float x,y;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ PSmoveto(xmid, ytop, outf);
+ x = Xpos + (xright-Xpos)/5.0; y = Ypos + 2.0*(ytop-Ypos)/3.0;
+ PSlineto(x,y,outf);
+ x = Xpos + 2.0*(xright-Xpos)/5.0;
+ PSlineto(x, y, outf);
+ PSlineto (x, Ypos, outf);
+ x = Xpos + 3.0*(xright-Xpos)/5.0;
+ PSlineto (x, Ypos, outf);
+ PSlineto(x, y, outf);
+ x = Xpos + 4.0*(xright-Xpos)/5.0;
+ PSlineto(x, y, outf);
+ PSlineto(xmid, ytop, outf);
+}
+
+/* Draw a small down arrow. mok 980917 */
+void ALDrawSmallDownArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float xmid;
+ float x,y;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ PSmoveto(xmid, Ypos, outf);
+ x = Xpos + 4.0*(xright-Xpos)/5.0; y = Ypos + (ytop-Ypos)/3.0;
+ PSlineto(x,y,outf);
+ x = Xpos + 3.0*(xright-Xpos)/5.0;
+ PSlineto(x, y, outf);
+ PSlineto (x, ytop, outf);
+ x = Xpos + 2.0*(xright-Xpos)/5.0;
+ PSlineto (x, ytop, outf);
+ PSlineto(x, y, outf);
+ x = Xpos + (xright-Xpos)/5.0;
+ PSlineto(x, y, outf);
+ PSlineto(xmid, Ypos, outf);
+}
+
+void ALDrawCircle(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSnewpath(outf);
+
+ fprintf(outf,"%.2f %.2f %.2f %d %d arc\n",xmid,ymid,(ytop - Ypos)*0.5,0,360);
+
+}
+
+/* Draw a diamond. mok 980917 */
+void ALDrawDiamond(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ PSmoveto(Xpos, ymid, outf);
+ PSlineto(xmid, ytop, outf);
+ PSlineto(xright, ymid, outf);
+ PSlineto(xmid, Ypos, outf);
+ PSlineto(Xpos, ymid, outf);
+}
+
+/* Draw a bar. mok 980917 */
+void ALDrawBar(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright;
+ float y1, y2;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+
+ y1 = Ypos + 2.0*(Ypos+Yspace)/5.0;
+ y2 = Ypos + 3.0*(Ypos+Yspace)/5.0;
+
+ PSmoveto(Xpos, y1, outf);
+ PSlineto(xright, y1, outf);
+ PSlineto(xright, y2, outf);
+ PSlineto(Xpos, y2, outf);
+ PSlineto(Xpos, y1, outf);
+}
+
+void ALDrawPseudoEllipse(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,
+ FILE *outf)
+
+{
+ /* draws a pseudo ellipse in the box bounded by Xpos,Ypos, Xpos+Xspace, Ypos+Yspace */
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+ float xefact = 0.2; /* this shifts the guide points by 0.2 towards the axis */
+ /* should not really be hard wired */
+ float yefact = 0.0;
+
+ float xbit,ybit;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+ xbit = Xspace * xefact;
+ ybit = Yspace * yefact;
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSnewpath(outf);
+
+ PSmoveto(xmid,Ypos,outf);
+
+ fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f curveto\n",
+ Xpos+xbit,Ypos+ybit,Xpos+xbit,ytop-ybit,xmid,ytop);
+ fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f curveto\n",
+ xright-xbit,ytop-ybit,xright-xbit,Ypos+ybit,xmid,Ypos);
+}
+
+void ALDrawLeftSemiEnd(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSnewpath(outf);
+
+ fprintf(outf,"%.2f %.2f %.2f %d %d arc\n",xmid,ymid,(ytop - Ypos) *0.5,270,90);
+
+ PSlineto(xright,ytop,outf);
+ PSlineto(xright,Ypos,outf);
+ PSlineto(xmid,Ypos,outf);
+
+}
+void ALDrawRightSemiEnd(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf)
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSnewpath(outf);
+ fprintf(outf,"%f %f %f %d %d arc\n",xmid,ymid,(ytop - Ypos) *0.5,90,270);
+
+ PSlineto(Xpos,Ypos,outf);
+ PSlineto(Xpos,ytop,outf);
+ PSlineto(xmid,ytop,outf);
+}
+void ALDrawRightHalfHorizLine(
+ float Xpos, /* bottom left hand corner of character posn */
+ float Ypos, /* */
+ float Xspace, /* horizontal width of character position */
+ float Yspace, /* vertical height of character position */
+ float width_fac, FILE *outf) /* proportion of vertical height that the line will fill */
+
+/* draw line from midpoint to right of position */
+
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ /* width_fac defines the width of the line in terms of the height of the characters */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSmoveto(xmid,ymid-hline_w,outf);
+ PSlineto(xmid,ymid+hline_w,outf);
+ PSlineto(xright,ymid+hline_w,outf);
+ PSlineto(xright,ymid-hline_w,outf);
+ PSlineto(xmid,ymid-hline_w,outf);
+}
+
+void ALDrawLeftHalfHorizLine(
+ float Xpos, /* bottom left hand corner of character posn */
+ float Ypos, /* */
+ float Xspace, /* horizontal width of character position */
+ float Yspace, /* vertical height of character position */
+ float width_fac, FILE *outf) /* proportion of vertical height that the line will fill */
+
+/* draw line from midpoint to left of position */
+
+{
+ float xright,ytop;
+ float ymid;
+ float hline_w;
+ float xmid;
+
+ xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */
+ ytop = Ypos + Yspace; /* top edge of character */
+ ymid = (ytop + Ypos) * 0.5; /* vertical mid point */
+ xmid = (xright + Xpos) * 0.5; /* horizontal mid point */
+
+ /* width_fac defines the width of the line in terms of the height of the characters */
+
+ hline_w = 0.5 * (width_fac * (ytop - Ypos));
+
+ PSmoveto(xmid,ymid-hline_w,outf);
+ PSlineto(xmid,ymid+hline_w,outf);
+ PSlineto(Xpos,ymid+hline_w,outf);
+ PSlineto(Xpos,ymid-hline_w,outf);
+ PSlineto(xmid,ymid-hline_w,outf);
+}
+
+void PSmoveto(float x,float y,FILE *outf)
+{
+ fprintf(outf,"%.2f %.2f moveto\n",x,y);
+}
+void PSlineto(float x,float y,FILE *outf)
+{
+ fprintf(outf,"%.2f %.2f lineto\n",x,y);
+}
+void PSPutSpecialText(float Xpos,float Ypos,float Xspace,float Yspace,char *text,
+ unsigned char current_col,
+ unsigned char background_colour,float YspaceFactor,FILE *outf)
+/* Handle the special text characters */
+{
+
+ Yspace *= YspaceFactor; /* shrink the Y axis a little to avoid clashes.*/
+
+
+ if(strcmp(text,"@horizline") == 0){
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fhorizline") == 0){
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@thickhorizline") == 0){
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fthickhorizline") == 0){
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ PSstroke(outf);
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@thinhorizline") == 0){
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fthinhorizline") == 0){
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ PSstroke(outf);
+ ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@rightarrow") == 0){
+ ALDrawRightArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@frightarrow") == 0){
+/* ALDrawRightArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);*/
+/* temporary change to the right arrow width 20/1/1995*/
+ ALDrawRightArrow(Xpos,Ypos,(Xspace*0.7),Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawRightArrow(Xpos,Ypos,(Xspace*0.7),Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@leftarrow") == 0){
+ ALDrawLeftArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fleftarrow") == 0){
+ ALDrawLeftArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawLeftArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@uparrow") == 0){
+ ALDrawUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fuparrow") == 0){
+ ALDrawUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@suparrow") == 0){
+ ALDrawSmallUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fsuparrow") == 0){
+ ALDrawSmallUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawSmallUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@downarrow") == 0){
+ ALDrawDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fdownarrow") == 0){
+ ALDrawDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@sdownarrow") == 0){
+ ALDrawSmallDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fsdownarrow") == 0){
+ ALDrawSmallDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawSmallDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@circle") == 0){
+ ALDrawCircle(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fcircle") == 0){
+ ALDrawCircle(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawCircle(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@diamond") == 0){
+ ALDrawDiamond(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fdiamond") == 0){
+ ALDrawDiamond(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawDiamond(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@bar") == 0){
+ ALDrawBar(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fbar") == 0){
+ ALDrawBar(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawBar(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@leftsemiend") == 0){
+ ALDrawLeftSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@fleftsemiend") == 0){
+ ALDrawLeftSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawLeftSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@rightsemiend") == 0){
+ ALDrawRightSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ }else if(strcmp(text,"@frightsemiend") == 0){
+ ALDrawRightSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+ ALDrawRightSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ }else if(strcmp(text,"@lefthelixend") == 0){
+
+ ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ PSstroke(outf);
+ ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",background_colour);
+
+ ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ PSstroke(outf);
+ ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,0.5,outf);
+ PSstroke(outf);
+
+ }else if(strcmp(text,"@righthelixend") == 0){
+
+ ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ PSstroke(outf);
+ ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ PSstroke(outf);
+ ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,1.0,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+ ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ PSstroke(outf);
+ ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf);
+ fprintf(outf,"closepath \n C%-d \n fill \n",current_col);
+
+
+ }else{
+ fprintf(stderr,"Error: Unrecognised special character: %s\n",text);
+ error(" ",1);
+ }
+}
+
+void PSstroke(FILE *outf)
+{
+ fprintf(outf,"stroke\n");
+}
+
+void PSnewpath(FILE *outf)
+{
+ fprintf(outf,"newpath\n");
+}
+
+void ALColourBackground(FILE *outf,int colour, int *b_region)
+{
+ int x1,y1,x2,y2;
+ x1 = b_region[0];
+ x2 = b_region[2];
+ y1 = b_region[1];
+ y2 = b_region[3];
+
+ fprintf(outf,"%d %d moveto %d %d lineto %d %d lineto %d %d lineto %d %d lineto closepath\n",
+ x1,y1,x2,y1,x2,y2,x1,y2,x1,y1);
+ fprintf(outf," C%-d \n fill \n",colour);
+ /* reset the colour to white */
+ /* fprintf(outf," C99\n");*/
+}
+
+void ALCheckLegalRange(int sx,int sy, int ex, int ey, int xmin, int ymin, int xmax, int ymax)
+/* check if sx, sy, ex, ey are within the limits given by xmin, ymin, xmax, ymax */
+{
+ extern FILE *std_err;
+ if( sx >= xmin && sx <= xmax
+ && sy >= ymin && sy <= ymax
+ && ex <= xmax && ex >= xmin
+ && ey <= ymax && ey >= ymin){
+ return;
+ }else{
+ fprintf(std_err,"\n\nALSCRIPT COMMAND FILE ERROR:\n");
+ fprintf(std_err,"Maximum legal range:\n");
+ fprintf(std_err,"From position %d of sequence %d to position %d of sequence %d\n",
+ xmin,ymin,xmax,ymax);
+
+ fprintf(std_err,"Your range:\n");
+ fprintf(std_err,"From position %d of sequence %d to position %d of sequence %d\n",
+ sx,sy,ex,ey);
+
+ GJerror("Range too big for alignment - try again!");
+ exit(0);
+ }
+}
+
+void ALMakeHelixText(int sx, int sy, int ex, char ***texts)
+{
+ int i;
+ texts[sx][sy] = GJstrdup("@lefthelixend");
+ texts[ex][sy] = GJstrdup("@righthelixend");
+ for(i=sx+1;i<ex;++i){
+ texts[i][sy] = GJstrdup("@fthickhorizline");
+ }
+}
+
+void ALMakeStrandText(int sx, int sy, int ex, char ***texts)
+{
+ int i;
+ texts[ex][sy] = GJstrdup("@frightarrow");
+ for(i=sx;i<ex;++i){
+ texts[i][sy] = GJstrdup("@fhorizline");
+ }
+}
+
+void ALMakeCoilText(int sx, int sy, int ex, char ***texts)
+{
+ int i;
+ for(i=sx;i<ex+1;++i){
+ texts[i][sy] = GJstrdup("@fthinhorizline");
+ }
+}
+
+int ALrel(char *pos,int *relnum)
+/* ALrel: parses a string that defines a position in a sequence. This can
+have the form
+
+to indicate position 10 10
+to indicate three before 10 10-3
+to indicate five after 10 10+5
+
+The routine looks up the relative number in *relnum before adding or
+subtracting numbers, the end result is therefore relative to the current
+alignment position.
+
+It should be easy to extend this routine for the relative movement to
+apply to positions in another sequence rather than alignment
+coordinates. (e.g. 10+5:73 might mean 5 after the current position in sequence 73).
+*/
+
+{
+ int i;
+ int len;
+ char *token;
+ int ipos,ioff; /* position and offset */
+ extern FILE *std_in,*std_out,*std_err;
+
+ len = strlen(pos);
+ ioff = 0;
+ ipos = 0;
+
+
+ if(strchr(pos,'+') != NULL){
+ token = strtok(pos,"+");
+ if(strlen(token)== 0){
+ error("Error decoding position",1);
+ fprintf(std_err,"Offending string is: %s\n",pos);
+ }else{
+ ipos = atoi(token);
+ }
+ token = strtok(NULL,"\0");
+ if(strlen(token)== 0){
+ error("Error decoding position",1);
+ fprintf(std_err,"Offending string is: %s\n",pos);
+ }else{
+ ioff = atoi(token);
+ }
+ return (relnum[ipos] + ioff);
+ }else if(strchr(pos,'-') != NULL){
+ token = strtok(pos,"-");
+ if(strlen(token)== 0){
+ error("Error decoding position",1);
+ fprintf(std_err,"Offending string is: %s\n",pos);
+ }else{
+ ipos = atoi(token);
+ }
+ token = strtok(NULL,"\0");
+ if(strlen(token)== 0){
+ error("Error decoding position",1);
+ fprintf(std_err,"Offending string is: %s\n",pos);
+ }else{
+ ioff = atoi(token);
+ }
+ return (relnum[ipos] - ioff);
+ }else{
+ ipos = atoi(pos);
+ return relnum[ipos];
+ }
+}
+
+int *ALCalCons(struct seqdat *bloc,
+ int sx,int sy,int ex,int ey,int *ret_val,int len)
+
+/* Calculate conservation for an alignment - this can be just a segment of an alignment */
+/* bloc = the alignment bloc */
+/* sx,sy,ex,ey = the positions over which to calculate the conservation */
+/* conscut = conservation number cutoff ( an integer 0 - 10) */
+/* ret_val = a pointer to an integer array to store the results of the calculation */
+/* len = the length of the array ret_val - this must be the same as the alignment length */
+
+{
+ int i,j,tlen,k;
+ char *temp;
+ char *temp2;
+ float tval;
+ int gcount;
+
+ tlen = ey -sy + 1;
+ temp = GJstrcreate(tlen+1,NULL);
+ if(ret_val == NULL){
+ ret_val = (int *) GJmalloc(sizeof(int) * len);
+ }
+
+ for(i=sx;i<(ex+1);++i){
+ /* calculate conservation at each position */
+ k = 0;
+ for(j=sy;j<(ey+1);++j){
+ temp[k] = bloc[j].seq[i];
+ ++k;
+ }
+ temp2 = GJstoupper(temp);
+ /* check for all gap columns */
+ gcount = 0;
+ for(j=0;j<tlen;++j){
+ if(temp2[j] == ' ') ++gcount;
+ }
+ if(gcount == tlen){
+ tval = 0.0;
+ }else{
+ tval = mzcons((unsigned char *)temp2,tlen);
+ }
+/* fprintf(stderr,"In calcons: i:%d tlen:%d temp:%s tval:%f\n",i,tlen,temp,tval);*/
+ ret_val[i] = 10 * tval;
+ GJfree(temp2);
+ }
+ GJfree(temp);
+ return ret_val;
+}
+
+float mzcons(unsigned char *pos,int n)
+/* calculate conservation value according to Zvelebil et al for a set
+ of amino acids and gaps stored in string pos of length n
+ translated from the fortran mzcons.f 6/3/1995
+*/
+
+{
+ /* -- conmat contains a table like that shown in the JMB paper
+ order of amino acids is GAP,ARNDCQEGHILKMFPSTWYVBZX
+ */
+ static char *acids = " ARNDCQEGHILKMFPSTWYVBZX";
+ static int conmat[24][10] = {
+ {1,1,1,1,1,1,1,1,1,1}, /* */
+ {1,0,0,0,0,1,1,0,0,0}, /*A */
+ {0,1,0,1,1,0,0,0,0,0}, /*R */
+ {0,0,0,1,0,1,0,0,0,0}, /*N */
+ {0,0,1,1,1,1,0,0,0,0}, /*D */
+ {1,0,0,0,0,1,0,0,0,0}, /*C */
+ {0,0,0,1,0,0,0,0,0,0}, /*Q */
+ {0,0,1,1,1,0,0,0,0,0}, /*E */
+ {1,0,0,0,0,1,1,0,0,0}, /*G */
+ {1,1,0,1,1,0,0,0,1,0}, /*H */
+ {1,0,0,0,0,0,0,1,0,0}, /*I */
+ {1,0,0,0,0,0,0,1,0,0}, /*L */
+ {1,1,0,1,1,0,0,0,0,0}, /*K */
+ {1,0,0,0,0,0,0,0,0,0}, /*M */
+ {1,0,0,0,0,0,0,0,1,0}, /*F */
+ {0,0,0,0,0,1,0,0,0,1}, /*P */
+ {0,0,0,1,0,1,1,0,0,0}, /*S */
+ {1,0,0,1,0,1,0,0,0,0}, /*T */
+ {1,0,0,1,0,0,0,0,1,0}, /*W */
+ {1,0,0,1,0,0,0,0,1,0}, /*Y */
+ {1,0,0,0,0,1,0,1,0,0}, /*V */
+ {0,0,0,1,0,0,0,0,0,0}, /*B */
+ {0,0,0,1,0,0,0,0,0,0}, /*Z */
+ {1,1,1,1,1,1,1,1,1,1} /*X */
+ };
+
+ int concnt,it,i,j,k,l,tcnt,ibseq;
+ int nide;
+ float con;
+ unsigned char facid;
+
+ concnt = 0;
+ for(j=0;j<10;++j){
+ /*found: outer loop over properties
+ set IT to the value of the first amino acid*/
+ it = conmat[GJindex(acids,pos[0])][j];
+ /*loop over remaining acids, if a difference occurs then
+ set tcnt to 1*/
+ tcnt = 0;
+ for(k=1;k<n;++k){
+ if(it != conmat[GJindex(acids,pos[k])][j]){
+ tcnt = 1;
+ }
+ }
+ /*add tcnt to concnt*/
+ concnt += tcnt;
+ }
+ /* check for total identity at this position*/
+ facid = pos[0];
+ nide = 0;
+ for(k=1;k<n;++k){
+ if(facid == pos[k]){
+ ++nide;
+ }
+ }
+
+ /*calculate the conservation*/
+ if(concnt == 10){
+ con = 0.0;
+ }else if(concnt == 0){
+ if(nide == (n-1)){
+ /*total identity*/
+ con = 1.0;
+ }else{
+ /*not identity, but same properties*/
+ con = 0.9;
+ }
+ }else{
+ con = 0.9 - 0.1 * concnt;
+ }
+ return con;
+}
+
+
+int GJindex(char *str,char c)
+{
+ char *t;
+ t = strchr(str,c);
+ if(t == NULL) return -1;
+ return (int) (t - str);
+}
+
--- /dev/null
+/****************************************************************************
+
+ ALSCRIPT - Sequence alignment to PostScript
+
+ Copyright: Geoffrey J. Barton (1992,1993,1997)
+ email: geoff@ebi.ac.uk
+
+ Please see the file README for limitations on the use of this program.
+
+All use of this program must cite: Barton, G. J. (1993), ALSCRIPT: A Tool to
+Format Multiple Sequence Alignments, Protein Engineering, Volume 6, No. 1,
+pp. 37-40.
+
+ $Id: alscript.c,v 1.2 1998/09/17 16:54:59 geoff Exp $
+ $Log: alscript.c,v $
+ Revision 1.2 1998/09/17 16:54:59 geoff
+ Check consistency with archive
+
+
+****************************************************************************
+
+ALSCRIPT is a program for producing pretty versions of multiple sequence aligments.
+ALSCRIPT will also format single sequences.
+A full description of the program is given in the file "alscript.doc".
+
+Ways of running alscript:
+
+1. Interactive mode: just type alscript.
+ You will be prompted for a command file name. The command file will
+ define the AMPS blocfile, and name of the file to store the PostScript
+ output - see alscript.doc for details.
+
+2. alscript <command_file> has same effect as 1, But does not prompt for the command file
+ e.g. alscript example1.com
+
+3. alscript -q < <blocfile> > <PostScript>
+ Quick mode - uses default commands, reads alignment from stdin, writes PostScript
+ to stdout.
+ e.g. alscript -q < example1.blc > example1.ps
+
+4. alscript -f <command_file>
+ Similar effect to 2.
+
+5. alscript -f <command_file> -s
+ Silent operation: No messages are written to stderr, unless fatal.
+ Silent operation may be toggled by the silent_mode command in the command file.
+
+6. alscript -f <command_file> -p < <blocfile> > <PostScript>
+ Make alscript work like a pipe - blocfile is read from stdin, postscript is written
+ to stdout. Messages are written to stderr. To supress messages include
+ the -s flag too
+ e.g. alscript -f example1.com -p -s < example1.blc > example1.ps
+
+Using alscript as a pipe has the advantage of allowing the blocfile to
+be created on the fly by the programs msf2blc or clus2blc. For example
+if we have a GCG .msf file called "pileup.msf" we can run alscript with
+default shading/fonts and send the results straight to the PostScript
+printer "lpr" as follows:
+
+msf2blc -q < pileup.msf | alscript -q -s | lpr
+
+See alps.c for revision history;
+fprintf(std_err,"\n Version 2.0 - 23 May 1995\n");
+
+*/
+
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include "array.h"
+#include "gjutil.h"
+#include "version.h"
+
+int MAXtlen = 500;
+int MAXnbloc = 500;
+int MAXilen = 50;
+int precis = 100;
+int MAXslen = 8000;
+int MAXnseq;
+
+void ALwritestddefs(FILE *);
+int alps(char *command_file,int silent, int pipe, int single);
+
+main(int argc,char *argv[])
+{
+ char *alps_file;
+ int silent;
+ int quick;
+ int i;
+ int pipe;
+ int singlepage; /* flag for EPS output */
+ FILE *fp;
+ char *Alscript_Version = ALSCRIPT_VERSION;
+
+ extern FILE *std_out,*std_err,*std_in;
+
+ silent = 0;
+ quick = 0;
+ pipe = 0;
+
+ alps_file = NULL;
+
+ /* initialize the file streams for GJ... routines */
+ GJinitfile();
+
+ singlepage = 0;
+
+ if(argc > 1){
+ /* check for -f option - if present, then define name for command file */
+ i = 1;
+ while(i<argc){
+ if(argv[i][0] == '-'){
+ if(strcmp(argv[i],"-f")==0){
+ /* command file name */
+ alps_file = GJstrdup(argv[++i]);
+ }else if(strcmp(argv[i],"-s")==0){
+ /* silent mode */
+ silent = 1;
+ }else if(strcmp(argv[i],"-q")==0){
+ /* quick mode - defines its own command file */
+ quick = 1;
+ pipe = 1;
+ }else if(strcmp(argv[i],"-p")==0){
+ /* send all output to std_out rather than the file defined in the command file */
+ pipe = 1;
+ silent = 1;
+ }else if(strcmp(argv[i],"-single")==0){
+ /* single page mode - will generate a BoundingBox */
+ singlepage = 1;
+ }
+ ++i;
+ }else if(i == 1){
+ /* we have just defined the alscript command file */
+ alps_file = GJstrdup(argv[1]);
+ break;
+ }
+ }
+ }
+
+ if(!silent){
+ fprintf(std_err,"\nALSCRIPT (ALignment to PostScript)\n");
+
+ fprintf(std_err,"%s\n",Alscript_Version);
+ fprintf(std_err,"\nSee ALSCRIPT.DOC for details\n");
+
+ fprintf(std_err,"\nPlease Reference: Barton, G. J. (1993), Protein Engineering, 6, 37-40.\n");
+
+ fprintf(std_err,"\nBy: G. J. Barton\n");
+ fprintf(std_err,"\nCopyright: Geoffrey J. Barton (1992,1997)\n");
+ fprintf(std_err,"email: geoff@ebi.ac.uk\n\n");
+
+ fprintf(std_err,"Initial Defaults:\n");
+ fprintf(std_err,"Maximum number of sequences:\t %d \t(Change using MAX_NSEQ command)\n",MAXnbloc);
+ fprintf(std_err,"Maximum sequence length:\t %d \t(Change using MAX_SEQ_LEN command)\n",MAXslen);
+ fprintf(std_err,"Maximum identifier length:\t %d \t(Change using MAX_ID_LEN command)\n",MAXilen);
+ }
+
+ if(alps_file == NULL && !quick){
+ fprintf(std_err,"Enter ALscript Command File name: ");
+ alps_file = (char *) GJmalloc(sizeof(char) * MAXtlen);
+ fscanf(std_in,"%s",alps_file);
+ }
+
+ if(quick){
+ /* create an alscript command file */
+ fp = GJfopen("ALPSQ.COM","w",1);
+ ALwritestddefs(fp);
+ GJfclose(fp,1);
+ alps_file = GJstrdup("ALPSQ.COM");
+ }
+
+ if(!silent)fprintf(std_err,"ALscript Command File: %s\n",alps_file);
+
+ if(alps(alps_file,silent,pipe,singlepage) == 1){
+ if(!silent)fprintf(std_err,"ALscript Finished\n");
+ exit(0);
+ }else{
+ error("Error in ALscript",1);
+ }
+}
+
+/* Write an alscript command file with some standard definitions */
+
+void ALwritestddefs(FILE *alps_file)
+{
+ fprintf(alps_file,"#ALSCRIPT - Default definitions\n");
+ fprintf(alps_file,"#For explanation of commands see file: alscript.doc\n");
+ fprintf(alps_file,"#Input from stdin and output to stdout by default\n");
+ fprintf(alps_file,"#block_file ?\n");
+ fprintf(alps_file,"#output_file ?\n");
+ fprintf(alps_file,"landscape\n");
+ fprintf(alps_file,"number_seqs\n");
+ fprintf(alps_file,"pointsize 8\n");
+ fprintf(alps_file,"define_font 0 Helvetica DEFAULT\n");
+ fprintf(alps_file,"define_font 1 Helvetica REL 0.5 \n");
+ fprintf(alps_file,"define_font 2 Helvetica-Bold DEFAULT\n");
+ fprintf(alps_file,"define_font 3 Times-Bold DEFAULT\n");
+ fprintf(alps_file,"define_font 4 Helvetica-BoldOblique DEFAULT\n");
+ fprintf(alps_file,"setup\n");
+ fprintf(alps_file,"mask SETUP\n");
+ fprintf(alps_file,"mask LEGAL \"ARNDCQEGHILKMFPSTWYVBZX\"\n");
+ fprintf(alps_file,"mask FRE ALL\n");
+ fprintf(alps_file,"mask SHADE ALL 0\n");
+ fprintf(alps_file,"mask INVERSE ALL\n");
+ fprintf(alps_file,"mask SHADE ALL 0\n");
+ fprintf(alps_file,"mask RESET\n");
+ fprintf(alps_file,"#shade_chars GP ALL 0\n");
+ fprintf(alps_file,"#inverse_chars GP ALL \n");
+ fprintf(alps_file,"#shade_chars LIVAWYFM ALL 0.8 \n");
+ fprintf(alps_file,"font_chars DEKRH ALL 4\n");
+}
+
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+$write sys$output "Setting up ALSCRIPT"
+$ass $b:[geoff.alscript] alsdir
+$alscript :== "run alsdir:alscript.exe"
+$write sys$output "Setting up msf2blc"
+$msf2blc :== "run alsdir:msf2blc.exe"
+$write sys$output "Setting up clus2blc"
+$clus2blc :== "run alsdir:clus2blc.exe"
+$write sys$output "Setting up alsnum"
+$alsnum :== "run alsdir:alsnum.exe"
+$write sys$output "ALSCRIPT setup"
--- /dev/null
+#include <stdlib.h>
+#include <stdio.h>
+#include "gjutil.h"
+#include "array.h"
+
+int Agetbloc(FILE *,struct seqdat *,int *);
+
+int MAXtlen = 500;
+int MAXnbloc = 500;
+int MAXilen = 50;
+int precis = 100;
+int MAXslen = 8000;
+int MAXnseq;
+
+/* alsnum: Aim - produce TEXT commands for ALSCRIPT that will generate numbers
+ corresponding to a given sequence in the blockfile.
+
+ Usage:
+
+ alsnum <seqnum> <startnum> <interval> <position> < blockfile > output
+
+ Where:
+
+ seqnum: The number of the sequence in the blockfile.
+
+ startnum: The number to use for the first residue in the sequence (e.g. 1).
+
+ interval: The numbering interval (e.g. 10).
+
+ position: Where to place the text in the alscript output (ie the Y coordinate).
+
+
+ ALL ARGUMENTS ARE REQUIRED
+
+
+ G. J. Barton (25 May 1993).
+
+ $Id: alsnum.c,v 1.2 1998/09/17 16:54:59 geoff Exp $
+ $Log: alsnum.c,v $
+ Revision 1.2 1998/09/17 16:54:59 geoff
+ Check consistency with archive
+
+
+*/
+
+main(int argc,char *argv[])
+
+{
+
+ extern FILE *std_in,*std_out,*std_err;
+ extern int MAXtlen,MAXnbloc;
+ extern int MAXilen,precis,MAXslen;
+
+ int seqnum,startnum,interval,position;
+ int nseq;
+ int *nums;
+ int nval;
+ int i;
+
+ struct seqdat *bloc;
+
+ GJinitfile();
+
+ if(argc != 5){
+ fprintf(std_err,
+ "Usage: alsnum <seqnum> <startnum> <interval> <position> < blockfile > output\n");
+ exit(0);
+ }
+
+ seqnum = atoi(argv[1]);
+ startnum = atoi(argv[2]);
+ interval = atoi(argv[3]);
+ position = atoi(argv[4]);
+
+ nseq = 0;
+ bloc = (struct seqdat *) malloc(sizeof(struct seqdat) * MAXnbloc);
+ mcheck((char *) bloc,"Cannot get space for bloc");
+ if(!Agetbloc(std_in,bloc,&nseq))error("Cannot read bloc file",1);
+
+ /* have read block file */
+
+ nums = (int *) GJmalloc(sizeof(int) * bloc[seqnum].slen);
+
+ nval = startnum;
+
+ /* get the array of numbers - use blank . or - as non-amino acid character */
+ for(i=1;i<bloc[seqnum].slen-1;++i){
+ if(bloc[seqnum].seq[i] == ' ' ||
+ bloc[seqnum].seq[i] == '.' ||
+ bloc[seqnum].seq[i] == '-' ){
+ nums[i] = 0;
+ }else{
+ nums[i] = nval;
+ ++nval;
+ }
+ }
+
+
+ /* now go down the array and output TEXT commands at interval intervals */
+
+ fprintf(std_out,"# Text commands created for numbering\n");
+ fprintf(std_out,"# Sequence Number: %d\n",seqnum);
+ fprintf(std_out,"# Starting Number: %d\n",startnum);
+ fprintf(std_out,"# Interval: %d\n",interval);
+ fprintf(std_out,"# Position: %d\n",position);
+
+ for(i=1;i<bloc[seqnum].slen-1;++i){
+ if(nums[i] != 0 && (float) (nums[i]/interval) == (float)nums[i]/(float)interval){
+ fprintf(std_out,"TEXT %d %d \"%d\"\n",i,position,nums[i]);
+ }
+ }
+
+ exit(0);
+}
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+/* Matrix file structure - eg. Dayhoffs matrix */
+
+struct pmatrix {
+ char *title; /* title of matrix */
+ char *indx; /* Index to matrix - ie amino acid codes */
+ int inlen; /* number of residues per line */
+ int **array; /* the actual pair score array */
+};
+
+/* Standard structure for storing protein sequence data */
+
+struct seqdat { /* all lengths include char terminator and [0] */
+ int ilen; /* length of identifier*/
+ char *id; /* identifier */
+ int tlen; /* length of title */
+ char *title; /* title */
+ int slen; /* length of sequence*/
+ char *seq; /* sequence */
+};
+
+/* smseq Structure to store the details of an alignment - the alignment
+itself is stored in an array of seqdat structures
+*/
+
+struct smseq {
+ int nseq; /* number of seqs in the alignment */
+ int blen; /* length of the alignment = no of residues +2 */
+ char *title; /* a name for the alignment */
+ int ninfo; /* number of lines of info */
+ char **info; /* optional information about the alignment */
+ char *gapchars; /* string of valid gap-characters */
+};
+
+
+/* mseq Structure for storing multiply aligned sequence data -
+ arranged so that
+ aligned positions in different sequences are adjacent
+ This structure could easily be extended to include additional annotations
+ (eg. the name of the alignment, its history, etc. etc.) can be added
+ without having to modify existing code (I hope..)
+ */
+
+struct sident {
+ char *id; /* sequence id */
+ char *title; /* sequence title */
+ int uid; /* unique identifier number for sequence */
+};
+
+struct alseq {
+ char *line; /* one position in the sequence alignment */
+};
+
+struct mseq {
+ int nseq; /* number of sequences in this aligned bloc */
+ int blen; /* overall aligned length of the bloc */
+ struct sident *itd; /* list of id,title,uid structures */
+ struct alseq *bloc; /* pointer to list of blen lines of the alignment
+ structure used to allow future expansion of info
+ on a per-aligned line basis (eg. presence of
+ flexible gap, etc)*/
+};
+
+/* structure to hold default values for id lengths etc. */
+struct defstr {
+ int maxilen; /* max id len */
+ int maxtlen; /* max title len */
+ int maxslen; /* max sequence length */
+};
+
+
+
+
+
--- /dev/null
+#include "array.h"
+
+void ckdd(struct seqdat *bloc,int nseq)
+{
+ return;
+}
--- /dev/null
+/***************************************************************************
+
+clus2blc: A program to convert a CLUSTAL PIR format alignment file to an
+ AMPS blockfile.
+
+ Copyright: Geoffrey J. Barton (1992,1997)
+
+ email: geoff@ebi.ac.uk
+ Please see the README file for details of conditions
+ for use of this program.
+
+ $Id: clus2blc.c,v 1.3 1999/07/09 10:35:28 geoff Exp $
+ $Log: clus2blc.c,v $
+ Revision 1.3 1999/07/09 10:35:28 geoff
+ Change version and copyright statement to reflect 1997 status
+
+ Revision 1.2 1998/09/17 16:55:00 geoff
+ Check consistency with archive
+
+
+
+****************************************************************************
+
+Notes: This program can be run as a pipe: type clus2blc -q < input > output
+Only error messages will be output to std_err
+
+Default mode is interactive and prompts for filenames.
+
+The storage for the sequences is allocated dynamically, so the MAX_SEQ_LEN
+defines in the header file "defaults.h" have no effect. If a system memory
+limit is reached, then a "malloc error" message will be written and the
+program will stop. Most computers should happily cope with large numbers of
+long sequences. If yours doesn't, some possible solutions are outlined in
+the user manual - alscript.doc.
+
+24 Oct 1994 - modify to remove dots and dashes from input file before writing
+output file (if -n option is second argument ).
+
+****************************************************************************/
+
+#include <stdio.h>
+#include <stdarg.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+
+#include "gjutil.h"
+#include "array.h"
+#include "defaults.h"
+
+#define TOKENS " \t\n"
+
+
+main(int argc,char *argv[])
+{
+ struct seqdat *seqs;
+ FILE *fp,*fout;
+ int nseq;
+ int found;
+ int i,j;
+ char *token,*sbit;
+ char *line;
+ extern FILE *std_err,*std_in,*std_out;
+ char *msffile;
+ char *blocfile;
+ int quiet;
+ char c;
+ int allen; /* total alignment length */
+ int nodot;
+
+ std_err = stderr;
+ std_in = stdin;
+ std_out = stdout;
+
+ line = GJstrcreate(MAX_INLEN," ");
+ msffile = GJstrcreate(MAX_INLEN,NULL);
+ blocfile = GJstrcreate(MAX_INLEN,NULL);
+
+ nseq = 0;
+ found = 0;
+ quiet = 0;
+ allen = 0;
+ nodot = 0;
+
+ if(argc > 1){
+ if(strcmp(argv[1],"-q")==0){
+ /* Quiet mode - read .MSF file from stdin and output block file to stdout */
+ quiet = 1;
+ fp = std_in;
+ fout = std_out;
+ }
+ if(argc > 2){
+ if(strcmp(argv[2],"-n")==0){
+ /* set flag to remove dots */
+ nodot = 1;
+ }
+ }
+ }else{
+ /* Verbose mode - prompt for all filenames */
+ fprintf(std_out,"\n\n");
+ fprintf(std_out,"CLUSTAL NBRF-PIR format to AMPS Blockfile conversion\n");
+ fprintf(std_out,"Copyright: G. J. Barton (1992)\n");
+ fprintf(std_out,"Author: G. J. Barton (1992)\n\n");
+ fprintf(std_out,"Max number/length of alignment - Defined by System\n");
+ fprintf(std_out,"If you get a malloc error message - see manual\n\n");
+ fprintf(std_out,"Enter CLUSTAL NBRF-PIR alignment filename: ");
+
+ fscanf(std_in,"%s",msffile);
+ fprintf(std_out,"Opening: %s\n",msffile);
+ fp = GJfopen(msffile,"r",1);
+
+ fprintf(std_out,"Enter Block filename: ");
+ fscanf(std_in,"%s",blocfile);
+ fprintf(std_out,"Opening: %s\n",blocfile);
+ fout = GJfopen(blocfile,"w",1);
+ }
+
+ fprintf(fout,"\n");
+ fprintf(fout,"Conversion of CLUSTAL NBRF-PIR file to AMPS BLOCKFILE format\n");
+ fprintf(fout,"clus2blc: Geoffrey J. Barton (1992)\n\n");
+
+ seqs = (struct seqdat *) GJmalloc(sizeof(struct seqdat));
+
+ if(!quiet)fprintf(std_out,"Reading .pir file\n");
+ nseq = 0;
+ while(fgets(line,MAX_INLEN,fp) != NULL){
+ if(line[0] == '>'){
+ /* found an identifier */
+ token = strtok(&line[1]," \n");
+ if(token != NULL){
+ seqs = (struct seqdat *) GJrealloc(seqs,sizeof(struct seqdat) * (nseq + 1));
+ seqs[nseq].id = GJstrdup(token);
+ if(fgets(line,MAX_INLEN,fp) != NULL){
+ /* read the title line */
+ seqs[nseq].title = GJstrdup(line);
+ seqs[nseq].seq = GJstrcreate(MAX_SEQ_LEN,NULL);
+ seqs[nseq].slen = 0;
+ seqs[nseq].seq = (char *) GJmalloc(sizeof(char));
+ i=0;
+ while((c = fgetc(fp)) != '*'){
+ /* read characters until * */
+ if(isalpha(c) || c == '-' || c == '.'){
+ seqs[nseq].seq = (char *) GJrealloc(seqs[nseq].seq,sizeof(char) * (i+1));
+ seqs[nseq].seq[i] = c;
+ ++i;
+ }else if(c == EOF){
+ break;
+ }
+ }
+ }
+ seqs[nseq].slen = i;
+ if(i > allen) allen = i;
+ ++nseq;
+ }
+ }
+ }
+
+ if(!quiet)fprintf(std_out,"All %d sequences read in\n",nseq);
+ if(!quiet)fprintf(std_out,"Writing .blc file\n");
+
+ for(i=0;i<nseq;++i){
+ fprintf(fout,">%s %s",seqs[i].id,seqs[i].title);
+ }
+ fprintf(fout,"* iteration 1\n");
+ for(i=0;i<allen;++i){
+ for(j=0;j<nseq;++j){
+ if(seqs[j].slen <= i){
+ fprintf(fout,"%c",' ');
+ }else{
+ /* edit out dots and dashes if required */
+ if(nodot == 1){
+ if(seqs[j].seq[i] == '.' || seqs[j].seq[i] == '-'){
+ seqs[j].seq[i] = ' ';
+ }
+ }
+ fprintf(fout,"%c",seqs[j].seq[i]);
+ }
+ }
+ fprintf(fout,"\n");
+ }
+ fprintf(fout,"*\n");
+ if(!quiet)fprintf(std_out,"All done\n");
+
+ for(i=0;i<nseq;++i){
+ GJfree(seqs[i].seq);
+ GJfree(seqs[i].id);
+ GJfree(seqs[i].title);
+ }
+ GJfree(seqs);
+ GJfree(line);
+ GJfree(blocfile);
+ GJfree(msffile);
+
+}
--- /dev/null
+/*****************************************************************************/
+ /* Defaults - all TOTAL lengths inc NULL*/
+
+#define MAX_ID_LEN 20 /* Protein ID Length */
+#define MAX_TITLE_LEN 500 /* Protein Title line */
+#define MAX_SEQ_LEN 8000 /* Sequence Length */
+#define MAX_NSEQ 1000 /* Number or Proteins */
+#define MAX_COM_LEN 20 /* maximum length for command words */
+#define MAX_MATRIX_SIDE 25 /* maximum dimension for pairscore matrix */
+#define MAX_BLOC_SEQ 500 /* max number of sequences is a blocfile */
+#define MAX_FILE_LEN 100 /* max number of characters in a filename */
+#define MAX_INFO 20
+#define MAX_INLEN 500 /* max input line length */
+
+#define INTSIZ sizeof(int)
+#define CSIZ sizeof(char)
+#define SQSIZ sizeof(struct seqdat)
+
+#define SMALL -100000
+
+
+
+
+
+
+
--- /dev/null
+! Makefile for ALSCRIPT, msf2blc and clus2blc programs
+!
+! These programs require an ANSI C compiler
+!
+! This makefile is for VAX/VMS systems that have mms
+!
+! module management system
+!
+CC = cc
+CFLAGS = /standard=ansi89/prefix=all/nolis
+ALOBJS = alscript.obj, alps.obj, gjutil.obj, agetbloc.obj, ckdd.obj
+!
+PROGS = alscript, msf2blc, clus2blc, alsnum
+
+all : $(PROGS)
+
+alscript : $(ALOBJS)
+ link/exe= alscript.exe $(ALOBJS)
+msf2blc : msf2blc.obj, gjutil.obj
+ link/exe= msf2blc.exe msf2blc.obj, gjutil.obj
+clus2blc : clus2blc.obj gjutil.obj
+ link/exe=clus2blc.exe clus2blc.obj, gjutil.obj
+alsnum : alsnum.obj, gjutil.obj, agetbloc.obj
+ link/exe=alsnum.exe alsnum.obj, gjutil.obj, agetbloc.obj
+
+alscript.obj : alscript.c, array.h, gjutil.h
+ $(CC) $(CFLAGS) alscript.c
+
+alps.obj : alps.c, array.h, gjutil.h
+ $(CC) $(CFLAGS) alps.c
+
+agetbloc.obj : agetbloc.c, array.h, gjutil.h
+ $(CC) $(CFLAGS) agetbloc.c
+
+gjutil.obj : gjutil.c, gjutil.h
+ $(CC) $(CFLAGS) gjutil.c
+
+clus2blc.obj : clus2blc.c, array.h, gjutil.h, defaults.h
+ $(CC) $(CFLAGS) clus2blc.c
+
+msf2blc.obj : msf2blc.c, array.h, gjutil.h, defaults.h
+ $(CC) $(CFLAGS) msf2blc.c
+
+ckdd.obj : ckdd.c, array.h
+ $(CC) $(CFLAGS) ckdd.c
+
+alsnum.obj : alsnum.c, array.h, gjutil.h, defaults.h
+ $(CC) $(CFLAGS) alsnum.c
--- /dev/null
+>one
+>two
+>three
+>four
+>five
+*
+ABCDE
+EDFTD
+AAAAA
+LLL
+PPPPP
+GGGGG
+SSSSS
+ABCDE
+EDFTD
+AAAAA
+LLL
+PPPPP
+GGGGG
+*
--- /dev/null
+/***************************************************************************
+
+fast2blc: A program to convert a FASTA format alignment file to an
+ AMPS blockfile.
+
+ Copyright: Geoffrey J. Barton (1992,1997)
+
+ email: geoff@ebi.ac.uk
+ Please see the README file for details of conditions for use of this program.
+
+ $Id: fast2blc.c,v 1.3 1999/07/09 10:35:29 geoff Exp $
+ $Log: fast2blc.c,v $
+ Revision 1.3 1999/07/09 10:35:29 geoff
+ Change version and copyright statement to reflect 1997 status
+
+ Revision 1.2 1998/09/17 16:55:01 geoff
+ Check consistency with archive
+
+
+****************************************************************************
+
+Notes: This program can be run as a pipe: type fast2blc -q < input > output
+Only error messages will be output to std_err
+
+Default mode is interactive and prompts for filenames.
+
+The storage for the sequences is allocated dynamically, so the MAX_SEQ_LEN
+defines in the header file "defaults.h" have no effect. If a system memory
+limit is reached, then a "malloc error" message will be written and the
+program will stop. Most computers should happily cope with large numbers of
+long sequences. If yours doesn't, some possible solutions are outlined in
+the user manual - alscript.doc.
+
+24 Oct 1994 - modify to remove dots and dashes from input file before writing
+output file (if -n option is second argument ).
+
+19 April 1995 - fast2blc derived from clus2blc to read a FASTA style alignment
+file. This assumes the title line is after the id code rather than on the
+next line.
+
+****************************************************************************/
+
+#include <stdio.h>
+#include <stdarg.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+
+#include "gjutil.h"
+#include "array.h"
+#include "defaults.h"
+
+#define TOKENS " \t\n"
+
+
+main(int argc,char *argv[])
+{
+ struct seqdat *seqs;
+ FILE *fp,*fout;
+ int nseq;
+ int found;
+ int i,j;
+ char *token,*sbit;
+ char *line;
+ extern FILE *std_err,*std_in,*std_out;
+ char *msffile;
+ char *blocfile;
+ int quiet;
+ char c;
+ int allen; /* total alignment length */
+ int nodot;
+
+ std_err = stderr;
+ std_in = stdin;
+ std_out = stdout;
+
+ line = GJstrcreate(MAX_INLEN," ");
+ msffile = GJstrcreate(MAX_INLEN,NULL);
+ blocfile = GJstrcreate(MAX_INLEN,NULL);
+
+ nseq = 0;
+ found = 0;
+ quiet = 0;
+ allen = 0;
+ nodot = 0;
+
+ if(argc > 1){
+ if(strcmp(argv[1],"-q")==0){
+ /* Quiet mode - read .MSF file from stdin and output block file to stdout */
+ quiet = 1;
+ fp = std_in;
+ fout = std_out;
+ }
+ if(argc > 2){
+ if(strcmp(argv[2],"-n")==0){
+ /* set flag to remove dots */
+ nodot = 1;
+ }
+ }
+ }else{
+ /* Verbose mode - prompt for all filenames */
+ fprintf(std_out,"\n\n");
+ fprintf(std_out,"FASTA format to AMPS Blockfile conversion\n");
+ fprintf(std_out,"Copyright: G. J. Barton (1992)\n");
+ fprintf(std_out,"Author: G. J. Barton (1992)\n\n");
+ fprintf(std_out,"Max number/length of alignment - Defined by System\n");
+ fprintf(std_out,"If you get a malloc error message - see manual\n\n");
+ fprintf(std_out,"Enter FASTA format alignment filename: ");
+
+ fscanf(std_in,"%s",msffile);
+ fprintf(std_out,"Opening: %s\n",msffile);
+ fp = GJfopen(msffile,"r",1);
+
+ fprintf(std_out,"Enter Block filename: ");
+ fscanf(std_in,"%s",blocfile);
+ fprintf(std_out,"Opening: %s\n",blocfile);
+ fout = GJfopen(blocfile,"w",1);
+ }
+
+ fprintf(fout,"\n");
+ fprintf(fout,"Conversion of FASTA file to AMPS BLOCKFILE format\n");
+ fprintf(fout,"fast2blc: Geoffrey J. Barton (1992)\n\n");
+
+ seqs = (struct seqdat *) GJmalloc(sizeof(struct seqdat));
+
+ if(!quiet)fprintf(std_out,"Reading .fas file\n");
+ nseq = 0;
+ while(fgets(line,MAX_INLEN,fp) != NULL){
+ if(line[0] == '>'){
+ /* found an identifier */
+ token = strtok(&line[1]," -\n");
+ if(token != NULL){
+ seqs = (struct seqdat *) GJrealloc(seqs,sizeof(struct seqdat) * (nseq + 1));
+ seqs[nseq].id = GJstrdup(token);
+ token = strtok(NULL,"\n");
+ if(token != NULL){
+ /* assign the title line */
+ seqs[nseq].title = GJstrdup(token);
+ seqs[nseq].seq = GJstrcreate(MAX_SEQ_LEN,NULL);
+ seqs[nseq].slen = 0;
+ seqs[nseq].seq = (char *) GJmalloc(sizeof(char));
+ i=0;
+ while((c = fgetc(fp)) != '*'){
+ /* read characters until * */
+ if(isalpha(c) || c == '-' || c == '.'){
+ seqs[nseq].seq = (char *) GJrealloc(seqs[nseq].seq,sizeof(char) * (i+1));
+ seqs[nseq].seq[i] = c;
+ ++i;
+ }else if(c == EOF){
+ break;
+ }
+ }
+ }else{
+ fprintf(std_err,
+ "Error: No title in sequence %s - is this FASTA format?\n",seqs[nseq].id);
+ exit(1);
+ }
+ seqs[nseq].slen = i;
+ if(i > allen) allen = i;
+ ++nseq;
+ }
+ }
+ }
+
+ if(!quiet)fprintf(std_out,"All %d sequences read in\n",nseq);
+ if(!quiet)fprintf(std_out,"Writing .blc file\n");
+
+ for(i=0;i<nseq;++i){
+ fprintf(fout,">%s %s\n",seqs[i].id,seqs[i].title);
+ }
+ fprintf(fout,"* iteration 1\n");
+ for(i=0;i<allen;++i){
+ for(j=0;j<nseq;++j){
+ if(seqs[j].slen <= i){
+ fprintf(fout,"%c",' ');
+ }else{
+ /* edit out dots and dashes if required */
+ if(nodot == 1){
+ if(seqs[j].seq[i] == '.' || seqs[j].seq[i] == '-'){
+ seqs[j].seq[i] = ' ';
+ }
+ }
+ fprintf(fout,"%c",seqs[j].seq[i]);
+ }
+ }
+ fprintf(fout,"\n");
+ }
+ fprintf(fout,"*\n");
+ if(!quiet)fprintf(std_out,"All done\n");
+
+ for(i=0;i<nseq;++i){
+ GJfree(seqs[i].seq);
+ GJfree(seqs[i].id);
+ GJfree(seqs[i].title);
+ }
+ GJfree(seqs);
+ GJfree(line);
+ GJfree(blocfile);
+ GJfree(msffile);
+
+}
--- /dev/null
+
+/****************************************************************************
+
+gjutil.c: Various utility routines - error checking malloc and
+free, string functions etc...
+
+Copyright: Geoffrey J. Barton (1992, 1993, 1995, 1997)
+email: geoff@ebi.ac.uk
+
+This software is made available for educational and non-commercial research
+purposes.
+
+For commercial use, a commercial licence is required - contact the author
+at the above address for details.
+
+******************************************************************************/
+#include <stdio.h>
+#include <stdarg.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+#include <errno.h>
+#include <time.h>
+
+#include <gjtimes.h>
+#include <gjutil.h>
+
+/* define pointers for standard streams to allow redefinition to files */
+
+FILE *std_err;
+FILE *std_in;
+FILE *std_out;
+
+/* clock externs */
+clock_t start_time, end_time,initial_time,final_time;
+
+void *GJmalloc(size_t size)
+/* malloc with simple error check */
+/* G. J. Barton 1992 */
+{
+ void *ptr;
+ ptr = (void *) malloc(size);
+ if(ptr == NULL){
+ GJerror("malloc error");
+ exit(0);
+ }
+ return ptr;
+}
+
+void *GJrealloc(void *ptr,size_t size)
+/* realloc with error check */
+/* G. J. Barton 1992 */
+{
+ ptr = (void *) realloc(ptr,size);
+ if(ptr == NULL){
+ GJerror("realloc error");
+ exit(0);
+ }
+ return ptr;
+}
+void *GJmallocNQ(size_t size)
+/* as for GJmalloc, but returns NULL on error*/
+/* G. J. Barton 1992 */
+{
+ void *ptr;
+ ptr = (void *) malloc(size);
+ if(ptr == NULL){
+ GJerror("malloc error");
+ return NULL;
+ }
+ return ptr;
+}
+
+void *GJreallocNQ(void *ptr,size_t size)
+/* as for GJrealloc with error check but returns NULL on error*/
+/* G. J. Barton 1992 */
+{
+ ptr = (void *) realloc(ptr,size);
+ if(ptr == NULL){
+ GJerror("realloc error");
+ return NULL;
+ }
+ return ptr;
+}
+void GJfree(void *ptr)
+/* free with error check */
+/* G. J. Barton 1992 */
+{
+ if(ptr == NULL){
+ GJerror("Attempt to free NULL pointer");
+ exit(0);
+ }
+ free(ptr);
+}
+
+void GJerror(const char *prefix)
+/* writes error message contained in prefix and contents of errno
+ to std_err.
+*/
+/* G. J. Barton 1992 */
+{
+ if(prefix != NULL){
+ if(*prefix != '\0'){
+ fprintf(std_err,"%s: ",prefix);
+ }
+ }
+ fprintf(std_err,"%s\n",strerror(errno));
+}
+
+/*
+error: calls GJerror
+*/
+void error(const char *str,int flag)
+{
+ GJerror(str);
+ if(flag)exit(0);
+}
+
+
+char *GJstoupper(const char *s)
+/* return a copy of s in upper case */
+/* G. J. Barton 1992 */
+{
+ char *temp;
+ int i;
+ temp = GJstrdup(s);
+ i=0;
+ while(temp[i] != '\0'){
+ temp[i] = toupper(temp[i]);
+ ++i;
+ }
+ return temp;
+}
+char *GJstolower(const char *s)
+/* return a copy of s in lower case */
+/* G. J. Barton 1992 */
+{
+ char *temp;
+ int i;
+ temp = GJstrdup(s);
+ i=0;
+ while(temp[i] != '\0'){
+ temp[i] = tolower(temp[i]);
+ ++i;
+ }
+ return temp;
+}
+char *GJstoup(char *s)
+/* return s in upper case */
+/* G. J. Barton 1992 */
+{
+ int i;
+ i=0;
+ while(s[i] != '\0'){
+ s[i] = toupper(s[i]);
+ ++i;
+ }
+ return s;
+}
+char *GJstolo(char *s)
+/* return s in lower case */
+/* G. J. Barton 1992 */
+{
+ int i;
+ i=0;
+ while(s[i] != '\0'){
+ s[i] = tolower(s[i]);
+ ++i;
+ }
+ return s;
+}
+
+char *GJstrdup(const char *s)
+/* returns a pointer to a copy of string s */
+/* G. J. Barton 1992 */
+
+{
+ char *temp;
+ temp = (char *) GJmalloc(sizeof(char) * (strlen(s)+1));
+ temp = strcpy(temp,s);
+ return temp;
+}
+
+char *GJstrrename(char *old,const char *new)
+/* takes old which is a pointer to a string, then replaces the contents
+ of the string with new, reallocating to the correct size
+*/
+{
+ int nlen;
+ nlen = strlen(new);
+ old = (char *) GJrealloc(old,sizeof(char) * (nlen + 1));
+ old = strcpy(old,new);
+ return old;
+}
+
+
+
+FILE *GJfopen(const char *fname,const char *type,int action)
+/* a file open function with error checking. The third argument
+is set to 0 if we want a failed open to return, or 1 if we
+want a failed open to exit the program.
+*/
+/* G. J. Barton 1992 */
+/* modified July 1995 - error message only printed if action is 1 */
+{
+ FILE *ret_val;
+ ret_val = fopen(fname,type);
+ if(ret_val == NULL){
+ /* GJerror(strcat("Cannot Open File: ",fname));*/
+ if(action == 1){
+ GJerror(strcat("Cannot Open File: ",fname));
+ exit(1);
+ }
+ }
+ return ret_val;
+}
+
+int GJfclose(FILE *fp,int action)
+/* a file close function with error checking. The second argument
+is set to 0 if we want a failed close to return, or 1 if we
+want a failed close to exit the program.
+*/
+/* G. J. Barton 1992 */
+{
+ int ret_val;
+ ret_val = fclose(fp);
+ if(ret_val != 0){
+ if(action == 1){
+ GJerror("Error closing File");
+ exit(1);
+ }
+ }
+ return ret_val;
+}
+
+
+GJFILE *GJfilemake(const char *name,const char *type,int action)
+/* If action = 1 then
+Tries to open the file with the given name. If successful returns
+a pointer to a struct file structure with the name and filehandle. If
+the open fails, or action= 0 then returns a struct file structure
+with the name and a NULL filehandle */
+/* G. J. Barton 1995 */
+{
+ GJFILE *ret_val;
+ ret_val = (GJFILE *) GJmalloc(sizeof(GJFILE));
+ ret_val->name = GJstrdup(name);
+ if(action == 1) {
+ ret_val->handle = GJfopen(ret_val->name,type,0);
+ }else if(action == 0){
+ ret_val->handle = NULL;
+ }
+ return ret_val;
+}
+
+GJFILE *GJfilerename(GJFILE *ret_val, const char *name)
+/* When passed the fval structure - renames the name part of the
+file structure to name, if the handle is non null it tries to close
+the file, then sets the file handle to NULL. */
+/* G. J. Barton 1995 */
+{
+ if(ret_val->name != NULL) {
+ GJfree(ret_val->name);
+ ret_val->name = GJstrdup(name);
+ }
+ if(ret_val->handle != NULL) {
+ GJfclose(ret_val->handle,0);
+ ret_val->handle = NULL;
+ }
+ return ret_val;
+}
+
+GJFILE *GJfileclose(GJFILE *ret_val,int action)
+/* Closes a file named in the struct file structure returns the struct
+ file structure */
+
+/* G. J. Barton July 1995 */
+{
+ STD_FILES;
+
+ if(GJfclose(ret_val->handle,0) == 0){
+ return ret_val;
+ }else {
+ if(action == 1){
+ GJerror("Error closing File");
+ fprintf(std_err,"%s\n",ret_val->name);
+ exit(-1);
+ }
+ }
+ return ret_val;
+}
+
+GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action)
+/* Opens a file named in the struct file structure */
+
+/* G. J. Barton October 1995 */
+{
+ STD_FILES;
+
+ ret_val->handle = GJfopen(ret_val->name,type,0);
+ if(ret_val->handle == NULL){
+ if(action == 1){
+ GJerror("Error opening File");
+ fprintf(std_err,"%s\n",ret_val->name);
+ exit(-1);
+ }
+ }
+ return ret_val;
+}
+
+GJFILE *GJfileclean(GJFILE *ret_val,int action)
+/* Closes the file then sets the file pointer to NULL, then
+ frees the filename string */
+
+/* G. J. Barton July 1995 */
+{
+ if(GJfclose(ret_val->handle,0) == 0) {
+ ret_val->handle = NULL;
+ GJfree(ret_val->name);
+ return ret_val;
+ }else {
+ if(action == 1){
+ GJerror("Error closing File");
+ fprintf(std_err,"%s\n",ret_val->name);
+ exit(-1);
+ }
+ }
+ return ret_val;
+}
+
+void GJinitfile(void)
+/* just set the standard streams */
+{
+ std_err = stderr;
+ std_in = stdin;
+ std_out = stdout;
+}
+
+char *GJfnonnull(char *string)
+/* return pointer to first non null character in the string */
+/* this could cause problems if the string is not null terminated */
+{
+ while(*string != '\0'){
+ ++string;
+ }
+ return ++string;
+}
+
+char *GJstrappend(char *string1, char *string2)
+/* appends string2 to the end of string2. Any newline characters are removed
+from string1, then the first character of string2 overwrites the null at the
+end of string1.
+string1 and string2 must have been allocated with malloc.
+*/
+/* G. J. Barton July 1992 */
+{
+ char *ret_val;
+ ret_val = GJremovechar(string1,'\n');
+ ret_val = (char *) GJrealloc(ret_val,
+ sizeof(char) * (strlen(ret_val) + strlen(string2) + 1));
+ ret_val = strcat(ret_val,string2);
+ return ret_val;
+}
+
+char *GJremovechar(char *string,char c)
+/* removes all instances of character c from string
+ returns a pointer to the reduced, null terminated string
+ 11/8/1996: couple of bugs found in this routine.
+ the length of the string returned was too short by 2 bytes.
+ This is a dodgy routine since string is freed.
+*/
+/* G. J. Barton (July 1992) */
+{
+ char *temp;
+ int j,i,nchar;
+ nchar = 0;
+ i=0;
+ while(string[i] != '\0'){
+ if(string[i] == c){
+ ++nchar;
+ }
+ ++i;
+ }
+ if(nchar == 0){
+ return string;
+ }else{
+ temp = (char *) GJmalloc(sizeof(char) * (strlen(string)-nchar + 1));
+ j=0;
+ i=0;
+ while(string[i] != '\0'){
+ if(string[i] != c){
+ temp[j] = string[i];
+ ++j;
+ }
+ ++i;
+ }
+ temp[j] = '\0';
+ GJfree(string);
+ return temp;
+ }
+}
+
+char *GJremovechar2(char *string,char c)
+/* removes all instances of character c from string
+ returns a pointer to the reduced, null terminated string
+*/
+/* G. J. Barton (July 1992) */
+{
+ char *temp;
+ int i,k,len;
+ k=0;
+ len=strlen(string);
+ temp = (char *) GJmalloc(sizeof(char) * (len+1));
+ for(i=0;i<(len+1);++i){
+ if(string[i] != c){
+ temp[k] = string[i];
+ ++k;
+ }
+ }
+ for(i=0;i<(strlen(temp)+1);++i){
+ string[i] = temp[i];
+ }
+ GJfree(temp);
+ return string;
+}
+
+
+char *GJsubchar(char *string,char c2,char c1)
+/* substitutes c1 for c2 in string
+*/
+/* G. J. Barton (July 1992) */
+{
+ int i;
+
+ i=0;
+ while(string[i] != '\0'){
+ if(string[i] == c1){
+ string[i] = c2;
+ }
+ ++i;
+ }
+ return string;
+}
+
+/* create a string and if fchar != NULL fill with characters */
+/* always set the len-1 character to '\0' */
+
+char *GJstrcreate(size_t len,char *fchar)
+{
+ char *ret_val;
+ ret_val = (char *) GJmalloc(sizeof(char) * len);
+ --len;
+ ret_val[len] = '\0';
+ if(fchar != NULL){
+ while(len > -1){
+ ret_val[len] = *fchar;
+ --len;
+ }
+ }
+ return ret_val;
+}
+
+/* searches for string s2 in string s1 and returns pointer to first instance
+of s2 in s1 or NULL if no instance found. s1 and s2 must be null terminated
+*/
+char *GJstrlocate(char *s1, char *s2)
+{
+ int i=0;
+ int j=0;
+ int k;
+ if(strlen(s1) == 0 || strlen(s2) == 0) return NULL;
+ while(i < strlen(s1)){
+ j=0;
+ k=i;
+ while(j < strlen(s2) && s1[k] == s2[j]){
+ ++k;
+ ++j;
+ }
+ if(j == strlen(s2)) return &s1[i];
+ ++i;
+ }
+ return NULL;
+}
+#include <stdlib.h>
+#include <string.h>
+
+
+/* GJstrtok()
+
+This version of strtok places the work pointer at the location of the first
+character in the next token, rather than just after the last character of the
+current token. This is useful for extracting quoted strings
+*/
+
+char *GJstrtok(char *input_string,const char *token_list)
+{
+ static char *work;
+ char *return_ptr;
+
+ if(input_string != NULL){
+ /* first call */
+ work = input_string;
+ }
+
+ /* search for next non-token character */
+ while(strchr(token_list,*work)!=NULL){
+ ++work;
+ }
+
+ if(*work == '\0'){
+ /* if we've reached the end of string, then return NULL */
+ return NULL;
+ }else{
+ return_ptr = (char *) work;
+ while(strchr(token_list,*work) == NULL){
+ if(*work == '\0'){
+ /* end of the string */
+ return return_ptr;
+ }else{
+ ++work;
+ }
+ }
+ *work = '\0';
+ ++work;
+ /* now increment work until we find the next non-delimiter character */
+ while(strchr(token_list,*work) != NULL){
+ if(*work == '\0'){
+ break;
+ }else{
+ ++work;
+ }
+ }
+ return return_ptr;
+ }
+}
+/**************************************************************************
+return a pointer to space for a rectangular unsigned character array
+Version 2.0 ANSI and uses GJmallocNQ
+--------------------------------------------------------------------------*/
+
+unsigned char **uchararr(int i,int j)
+{
+ unsigned char **temp;
+ int k, rowsiz;
+
+ temp = (unsigned char **) GJmallocNQ(sizeof(unsigned char *) * i);
+ if(temp == NULL) return NULL;
+
+ rowsiz = sizeof(unsigned char) * j;
+
+ for (k = 0; k < i; ++k){
+ temp[k] = (unsigned char *) GJmallocNQ(rowsiz);
+ if(temp[k] == NULL) return NULL;
+ }
+ return temp;
+}
+
+/**************************************************************************
+free up space pointed to by rectangular unsigned character array
+-------------------------------------------------------------------------*/
+void ucharfree(unsigned char **array,int i)
+
+{
+ int k;
+
+ for (k = 0; k < i; ++k){
+ GJfree((char *) array[k]);
+ }
+ GJfree((char *) array);
+
+}
+
+/**************************************************************************
+return a pointer to space for a rectangular signed character array
+Version 2.0 ANSI
+--------------------------------------------------------------------------*/
+signed char **chararr(int i,int j)
+
+{
+ signed char **temp;
+ int k, rowsiz;
+
+ temp = (signed char **) GJmallocNQ(sizeof(char *) * i);
+
+ if(temp == NULL) return NULL;
+
+ rowsiz = sizeof(char) * j;
+
+ for (k = 0; k < i; ++k){
+ temp[k] = (signed char *) GJmallocNQ(rowsiz);
+ if(temp[k] == NULL) return NULL;
+ }
+ return temp;
+}
+
+
+/* mcheck - check a call to malloc - if the call has failed, print the
+error message and exit the program */
+/* ANSI Version - also uses GJerror routine and ptr is declared void*/
+
+void mcheck(void *ptr,char *msg)
+
+{
+ if(ptr == NULL){
+ GJerror("malloc/realloc error");
+ exit(0);
+ }
+}
+
+/* set a string to blanks and add terminating nul */
+char *GJstrblank(char *string,int len)
+
+{
+ --len;
+ string[len] = '\0';
+ --len;
+ while(len > -1){
+ string[len] = ' ';
+ --len;
+ }
+ return string;
+}
+
+/* Initialise an unsigned char array */
+void GJUCinit(unsigned char **array,int i,int j,unsigned char val)
+{
+ int k,l;
+
+ for(k=0;k<i;++k){
+ for(l=0;l<j;++l){
+ array[k][l] = val;
+ }
+ }
+}
+/*Initialise a signed char array */
+
+void GJCinit(signed char **array,int i,int j,char val)
+
+{
+ int k,l;
+
+ for(k=0;k<i;++k){
+ for(l=0;l<j;++l){
+ array[k][l] = val;
+ }
+ }
+}
+
+/* Initialise an integer vector */
+void GJIinit(int *array,int i,int val)
+{
+ int k;
+
+ for(k=0;k<i;++k){
+ array[k] = val;
+ }
+}
+
+/******************************************************************
+GJcat: concatenate N NULL terminated strings into a single string.
+The source strings are not altered
+Author: G. J. Barton (Feb 1993)
+------------------------------------------------------------------*/
+char *GJcat(int N,...)
+{
+ va_list parminfo;
+ int i,j,k;
+ char **values; /*input strings */
+ int *value_len; /*lengths of input strings */
+ int ret_len; /*length of returned string */
+ char *ret_val; /*returned string */
+
+ ret_len = 0;
+ values = (char **) GJmalloc(sizeof(char *) * N);
+ value_len = (int *) GJmalloc(sizeof(int *) * N);
+
+ va_start(parminfo,N);
+
+ /* Get pointers and lengths for the N arguments */
+ for(i=0;i<N;++i){
+ values[i] = va_arg(parminfo,char *);
+ value_len[i] = strlen(values[i]);
+ ret_len += value_len[i];
+ }
+
+ ret_val = (char *) GJmalloc(sizeof(char) * (ret_len+1));
+
+ /* Transfer input strings to output string */
+ k=0;
+ for(i=0;i<N;++i){
+ for(j=0;j<value_len[i];++j){
+ ret_val[k] = values[i][j];
+ ++k;
+ }
+ }
+ ret_val[k] = '\0';
+ GJfree(values);
+ GJfree(value_len);
+
+ va_end(parminfo);
+
+ return ret_val;
+}
+
+/************************************************************************
+
+GJGetToken:
+
+The aim of this routine is to emulate the strtok() function, but reading
+from a file. The functionality may differ slightly...
+
+Characters are read from the file until a character that is found in
+delim is encountered. When this occurs, token is returned. If the
+file consists entirely of delimiters, then token is freed
+and NULL is returned. Similarly, if end of file is encountered.
+
+------------------------------------------------------------------------*/
+
+
+char *GJGetToken(FILE *in, const char *delim)
+
+{
+ int i;
+ int c;
+ char *token;
+
+ i=0;
+
+ token = GJmalloc(sizeof(char));
+
+ while((c=fgetc(in)) != EOF){
+ if(strchr(delim,c) == NULL){
+ /* not a delimiter */
+ token[i++] = c;
+ token = GJrealloc(token,sizeof(char) * (i+1));
+ }else if(i>0){
+ token[i] = '\0';
+ return token;
+ }
+ }
+/* GJerror("End of File Encountered");*/
+ GJfree(token);
+ return NULL;
+}
+
+struct tokens *GJgettokens(const char *delims, char *buff)
+/* This splits a buffer into tokens at each position defined by delims.*/
+/* The structure returned contains the number of tokens and the */
+/* tokens themselves as a char ** array */
+{
+ char *token;
+ struct tokens *tok;
+
+ token = strtok(buff,delims);
+ if(token == NULL) return NULL;
+
+ tok = (struct tokens *) GJmalloc(sizeof(struct tokens));
+ tok->ntok = 0;
+ tok->tok = (char **) GJmalloc(sizeof(char *));
+ tok->tok[0] = GJstrdup(token);
+ ++tok->ntok;
+ while((token = strtok(NULL,delims)) != NULL) {
+ tok->tok = (char **) GJrealloc(tok->tok,sizeof(char *) * (tok->ntok+1));
+ tok->tok[tok->ntok] = GJstrdup(token);
+ ++tok->ntok;
+ }
+
+ return tok;
+}
+
+void GJfreetokens(struct tokens *tok)
+/* frees a tokens structure */
+
+{
+ int i;
+ for(i=0;i<tok->ntok;++i) {
+ GJfree(tok->tok[i]);
+ }
+ GJfree(tok->tok);
+ GJfree(tok);
+ tok = NULL; /* add this to avoid odd pointer 27/6/1997*/
+}
+
+char * GJtoktostr(struct tokens *tok,char delim,int s, int e)
+
+/*
+ returns a string with the tokens between s and e inclusive written to
+ it separated by delim
+ the tok structure is unmodified.
+*/
+
+{
+ int n, i, j,k;
+ char *ret_val;
+
+ n = 0;
+
+
+ if(s < 0 || s >= tok->ntok) s = 0;
+ if(e < 0 || e >= tok->ntok) e = tok->ntok - 1;
+
+ for(i=s;i<=e;++i){
+ n += strlen(tok->tok[i]);
+ ++n;
+ }
+
+ ret_val = (char *) GJmalloc(sizeof(char) * n);
+ j = 0;
+ for(i=s;i<=e;++i){
+ for(k=0;k<strlen(tok->tok[i]);++k){
+ ret_val[j] = tok->tok[i][k];
+ ++j;
+ }
+ ret_val[j++] = delim;
+ }
+ ret_val[n-1] = '\0';
+ return ret_val;
+}
+
+/*
+void GJ_start_clock()
+{
+ extern clock_t start_time, end_time,initial_time,final_time;
+ start_time = clock();
+ initial_time = start_time;
+}
+
+void GJ_stop_clock(FILE *fp)
+{
+ extern clock_t start_time, end_time,initial_time,final_time;
+ end_time = clock();
+ fprintf(fp,"CPU time: %f seconds\n",
+ ((float) (end_time - start_time))/CLOCKS_PER_SEC);
+}
+*/
+
+
+void GJ_start_clock(void)
+{
+ GJ_init_times();
+}
+
+void GJ_stop_clock(FILE *fp)
+{
+ GJ_show_times(fp,3);
+ GJ_reset_times();
+}
+
+
+void GJindexx(int *arrin,int n,int *indx)
+ /* indexed heap sort - adapted from the NR routine indexx.
+ inarr is an integer array to sort,
+ indx is the returned index array
+ */
+{
+ int l,j,ir,indxt,i;
+ float q;
+
+ for (j=1;j<=n;j++) indx[j]=j;
+ l=(n >> 1) + 1;
+ ir=n;
+ for (;;) {
+ if (l > 1)
+ q=arrin[(indxt=indx[--l])];
+ else {
+ q=arrin[(indxt=indx[ir])];
+ indx[ir]=indx[1];
+ if (--ir == 1) {
+ indx[1]=indxt;
+ return;
+ }
+ }
+ i=l;
+ j=l << 1;
+ while (j <= ir) {
+ if (j < ir && arrin[indx[j]] < arrin[indx[j+1]]) j++;
+ if (q < arrin[indx[j]]) {
+ indx[i]=indx[j];
+ j += (i=j);
+ }
+ else j=ir+1;
+ }
+ indx[i]=indxt;
+ }
+}
+
+
+
+void GJpline(FILE *fp,char c,int n)
+/* print n copies of c to fp terminated by newline */
+{
+ int i;
+ for(i=0;i<n;++i){
+ fprintf(fp,"%c",c);
+ }
+ fprintf(fp,"\n");
+}
+
+char *GJlocaltime(void)
+{
+ time_t t;
+ char *ret_val;
+ t = time(NULL);
+ ret_val=asctime(localtime(&t));
+ return ret_val;
+}
+
+void GJpstring(FILE *fp,char *s,int n)
+/* print a string so that it occupies n characters */
+/* strings are left-justified and either truncated or padded on output */
+{
+ int i,l;
+
+
+ l = strlen(s);
+
+ if(l > n){
+ for(i=0;i<n;++i){
+ fprintf(fp,"%c",s[i]);
+ }
+ }else{
+ for(i=0;i<l;++i){
+ fprintf(fp,"%c",s[i]);
+ }
+ for(i=l;i<n;++i){
+ fprintf(fp," ");
+ }
+ }
+}
+
+/* return min and max of an integer vector */
+IRANGE *irange(int *ivec,int n)
+{
+ int i;
+ int imin,imax;
+ IRANGE *ret_val;
+
+ imin = imax = ivec[0];
+
+ for(i=1;i<n;++i){
+ if(ivec[i] > imax) imax = ivec[i];
+ if(ivec[i] < imin) imin = ivec[i];
+ }
+
+ ret_val = (IRANGE *) GJmalloc(sizeof(IRANGE));
+ ret_val->min = imin;
+ ret_val->max = imax;
+
+ return ret_val;
+}
+
+/*
+
+$Id: gjutil.c,v 1.2 1998/09/17 16:55:01 geoff Exp $
+
+$Log: gjutil.c,v $
+Revision 1.2 1998/09/17 16:55:01 geoff
+Check consistency with archive
+
+Revision 1.8 1998/08/11 15:38:50 geoff
+Modified the copyright notice to reflect the new
+ownership of this software.
+
+Revision 1.7 1997/06/29 00:43:57 gjb
+Changes to add sysinfo calls and test of license reading routines
+
+Revision 1.6 1997/06/27 16:42:41 gjb
+Add trlic.c test.lic and clean up gjutil.c
+
+Revision 1.5 1997/06/27 07:17:31 gjb
+Added rlic.c linfo.h and
+changes to gjutil.c to give better support for
+token manipulation
+
+Revision 1.4 1997/05/12 11:10:53 gjb
+Re-added gjutil.c and gjutil.h to repository
+after deleting them
+
+Revision 1.2 1997/05/12 10:47:52 gjb
+Modified CVS header and log position
+
+*/
--- /dev/null
+#ifndef GJ_UTIL_H
+#define GJ_UTIL_H
+
+/*
+$Id: gjutil.h,v 1.2 1998/09/17 16:55:02 geoff Exp $
+*/
+
+#include <stdio.h>
+
+
+typedef int GJ_LIM; /* type for limits - e.g. max buffer length */
+typedef int GJ_PEN; /* type for gap penalties */
+typedef int GJ_FLG; /* Flag type - usually just 1 or 0 */
+typedef float GJ_FLOAT; /* Single precision floating point type */
+typedef double GJ_DBL; /* a double precision floating point type */
+typedef int GJ_S_COUNT; /* small counter */
+typedef long GJ_L_COUNT; /* long counter */
+
+typedef struct { /* structure to hold a filename and */
+ char *name; /* associated handle */
+ FILE *handle;
+} GJFILE;
+
+struct tokens { /* structure to hold tokens parsed from */
+ int ntok; /* string with strtok */
+ char **tok;
+};
+
+typedef struct {
+ int min;
+ int max;
+} IRANGE;
+
+#define STD_FILES extern FILE *std_in,*std_out,*std_err
+
+/* utility.h function definitions */
+
+void *GJmalloc(size_t);
+void *GJrealloc(void *,size_t);
+void *GJmallocNQ(size_t);
+void *GJreallocNQ(void *,size_t);
+void GJfree(void *);
+void GJerror(const char *);
+char *GJstrdup(const char *);
+char *GJstoupper(const char *);
+char *GJstolower(const char *);
+char *GJstoup(char *);
+char *GJstolo(char *);
+
+FILE *GJfopen(const char *, const char *,int);
+int GJfclose(FILE *,int);
+GJFILE *GJfilemake(const char *name,const char *type,int action);
+GJFILE *GJfilerename(GJFILE *ret_val, const char *name);
+GJFILE *GJfileclose(GJFILE *ret_val,int action);
+GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action);
+GJFILE *GJfileclean(GJFILE *fval,int action);
+void GJinitfile(void);
+
+char *GJfnonnull(char *);
+char *GJstrappend(char *,char *);
+char *GJremovechar(char *,char);
+char *GJremovechar2(char *string,char c);
+char *GJstrcreate(size_t, char *);
+char *GJstrlocate(char *,char *);
+char *GJsubchar(char *,char,char);
+char *GJstrtok(char *,const char *);
+void error(const char *, int);
+unsigned char **uchararr(int,int);
+void ucharfree(unsigned char **array,int i);
+signed char **chararr(int,int);
+void GJCinit(signed char **,int ,int ,char );
+void mcheck(void *, char *);
+char *GJstrblank(char *, int);
+void GJUCinit(unsigned char **,int ,int ,unsigned char );
+char *GJcat(int N,...);
+struct tokens *GJgettokens(const char *delims, char *buff);
+void GJfreetokens(struct tokens *tok);
+char * GJtoktostr(struct tokens *tok, char delim, int s, int e);
+void GJ_start_clock(void);
+void GJ_stop_clock(FILE *fp);
+char *GJstrrename(char *old,const char *new);
+void GJindexx(int *arrin,int n,int *indx);
+
+void GJpline(FILE *fp,char c,int n);
+char *GJlocaltime(void);
+void GJpstring(FILE *fp,char *s,int n);
+
+IRANGE *irange(int *ivec, int n);
+
+/*
+$Log: gjutil.h,v $
+Revision 1.2 1998/09/17 16:55:02 geoff
+Check consistency with archive
+
+Revision 1.5 1997/06/27 07:17:32 gjb
+Added rlic.c linfo.h and
+changes to gjutil.c to give better support for
+token manipulation
+
+Revision 1.4 1997/05/12 11:10:54 gjb
+Re-added gjutil.c and gjutil.h to repository
+after deleting them
+
+Revision 1.2 1997/05/12 10:47:52 gjb
+Modified CVS header and log position
+
+*/
+#endif /* GJ_UTIL_H */
+
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for gcc linux computers it should need only
+# minor modifications to work on other computer types, e.g. Sun
+# Dec etc. This also appears to work under cygwin, though you
+# have to use the --unix flag to make.
+#
+#
+CC = gcc
+MACHINE = linux
+CFLAGS = -O2 -I./ -I../gjutil/
+LFLAGS = -o
+ALOBJS = alscript.o alps.o ../gjutil/gjutil.o agetbloc.o ckdd.o ../gjutil/gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) fast2blc
+alsnum: alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o $(LFLAGS) alsnum
+
+
+alscript.o: alscript.c array.h ../gjutil/gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h ../gjutil/gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h ../gjutil/gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+../gjutil/gjutil.o: ../gjutil/gjutil.c ../gjutil/gjutil.h ../gjutil/gjtimes.o
+ (cd ../gjutil; $(CC) $(CFLAGS) -c gjutil.c gjtimes.o ; cd ../src)
+
+../gjutil/gjtimes.o: ../gjutil/gjtimes.c ../gjutil/gjtimes.h
+ (cd ../gjutil; $(CC) $(CFLAGS) -c gjtimes.c ; cd ../src)
+
+clus2blc.o: clus2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+msf2blc.o: msf2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+fast2blc.o: fast2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c fast2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for Sun computers
+#
+CC = gcc
+MACHINE = gcc
+CFLAGS = -O2 -I./
+LFLAGS = -o
+ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o gjutil.o
+ $(CC) $(CFLAGS) msf2blc.o gjutil.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o gjutil.o
+ $(CC) $(CFLAGS) clus2blc.o gjutil.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o gjutil.o
+ $(CC) $(CFLAGS) fast2blc.o gjutil.o $(LFLAGS) fast2blc
+alsnum: alsnum.o gjutil.o agetbloc.o
+ $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o $(LFLAGS) alsnum
+
+alscript.o: alscript.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+gjutil.o: gjutil.c gjutil.h gjtimes.o
+ $(CC) $(CFLAGS) -c gjutil.c
+
+gjtimes.o: gjtimes.c gjtimes.h
+ $(CC) $(CFLAGS) -c gjtimes.c
+
+clus2blc.o: clus2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+msf2blc.o: msf2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for gcc linux computers it should need only
+# minor modifications to work on other computer types, e.g. Sun
+# Dec etc. This also appears to work under cygwin, though you
+# have to use the --unix flag to make.
+#
+#
+CC = gcc
+MACHINE = linux
+CFLAGS = -O2 -I./ -I../gjutil/
+LFLAGS = -o
+ALOBJS = alscript.o alps.o ../gjutil/gjutil.o agetbloc.o ckdd.o ../gjutil/gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) fast2blc
+alsnum: alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o $(LFLAGS) alsnum
+
+
+alscript.o: alscript.c array.h ../gjutil/gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h ../gjutil/gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h ../gjutil/gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+../gjutil/gjutil.o: ../gjutil/gjutil.c ../gjutil/gjutil.h ../gjutil/gjtimes.o
+ (cd ../gjutil; $(CC) $(CFLAGS) -c gjutil.c gjtimes.o ; cd ../src)
+
+../gjutil/gjtimes.o: ../gjutil/gjtimes.c ../gjutil/gjtimes.h
+ (cd ../gjutil; $(CC) $(CFLAGS) -c gjtimes.c ; cd ../src)
+
+clus2blc.o: clus2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+msf2blc.o: msf2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+fast2blc.o: fast2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c fast2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# 22 June 1993: Add alsnum program
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for Sun computers
+#
+CC = cc
+MACHINE = osf
+CFLAGS = -O2 -Olimit 1500 -I./
+LFLAGS = -o
+ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) msf2blc.o gjutil.o gjtimes.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) clus2blc.o gjutil.o gjtimes.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) fast2blc.o gjutil.o gjtimes.o $(LFLAGS) fast2blc
+alsnum: alsnum.o gjutil.o agetbloc.o gjtimes.o
+ $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o gjtimes.o $(LFLAGS) alsnum
+
+
+alscript.o: alscript.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+gjutil.o: gjutil.c gjutil.h gjtimes.o
+ $(CC) $(CFLAGS) -c gjutil.c gjtimes.o
+
+gjtimes.o: gjtimes.c gjtimes.h
+ $(CC) $(CFLAGS) -c gjtimes.c
+
+clus2blc.o: clus2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+msf2blc.o: msf2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for Silicon Graphics computers it should need only
+# minor modifications to work on other computer types, e.g. Sun
+# Dec etc.
+#
+CC = cc
+MACHINE = sgi
+CFLAGS = -O1 -I./ -I../gjutil/
+LFLAGS = -o
+ALOBJS = alscript.o alps.o ../gjutil/gjutil.o agetbloc.o ckdd.o ../gjutil/gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) fast2blc
+alsnum: alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o
+ $(CC) $(CFLAGS) alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o $(LFLAGS) alsnum
+
+
+alscript.o: alscript.c array.h ../gjutil/gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h ../gjutil/gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h ../gjutil/gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+../gjutil/gjutil.o: ../gjutil/gjutil.c ../gjutil/gjutil.h ../gjutil/gjtimes.o
+ (cd ../gjutil; $(CC) $(CFLAGS) -c gjutil.c gjtimes.o ; cd ../src)
+
+../gjutil/gjtimes.o: ../gjutil/gjtimes.c ../gjutil/gjtimes.h
+ (cd ../gjutil; $(CC) $(CFLAGS) -c gjtimes.c ; cd ../src)
+
+clus2blc.o: clus2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+msf2blc.o: msf2blc.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h ../gjutil/gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# 22 June 1993: Add alsnum program
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for Sun computers running Solaris 2.x
+#
+CC = cc
+MACHINE = sol
+CFLAGS = -O
+LFLAGS = -I./ -o
+ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) msf2blc.o gjutil.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) clus2blc.o gjutil.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) fast2blc.o gjutil.o $(LFLAGS) fast2blc
+alsnum: alsnum.o gjutil.o agetbloc.o gjtimes.o
+ $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o gjtimes.o $(LFLAGS) alsnum
+
+alscript.o: alscript.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+gjutil.o: gjutil.c gjutil.h
+ $(CC) $(CFLAGS) -c gjutil.c
+
+clus2blc.o: clus2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+fast2blc.o: fast2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c fast2blc.c
+
+msf2blc.o: msf2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
+
+
+
+
+
+
+
+
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# 22 June 1993: Add alsnum program
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for Sun computers
+#
+CC = acc
+MACHINE = sun
+CFLAGS = -O2 -I./
+LFLAGS = -o
+ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o
+#
+PROGS = alscript msf2blc clus2blc alsnum fast2blc
+
+all: $(PROGS)
+
+alscript: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript
+msf2blc: msf2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) msf2blc.o gjutil.o gjtimes.o $(LFLAGS) msf2blc
+clus2blc: clus2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) clus2blc.o gjutil.o gjtimes.o $(LFLAGS) clus2blc
+fast2blc: fast2blc.o gjutil.o gjtimes.o
+ $(CC) $(CFLAGS) fast2blc.o gjutil.o gjtimes.o $(LFLAGS) fast2blc
+alsnum: alsnum.o gjutil.o agetbloc.o gjtimes.o
+ $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o gjtimes.o $(LFLAGS) alsnum
+
+
+alscript.o: alscript.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alscript.c
+
+alps.o: alps.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) -c alps.c
+
+agetbloc.o: agetbloc.c array.h gjutil.h
+ $(CC) $(CFLAGS) -c agetbloc.c
+
+gjutil.o: gjutil.c gjutil.h gjtimes.o
+ $(CC) $(CFLAGS) -c gjutil.c gjtimes.o
+
+gjtimes.o: gjtimes.c gjtimes.h
+ $(CC) $(CFLAGS) -c gjtimes.c
+
+clus2blc.o: clus2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c clus2blc.c
+
+msf2blc.o: msf2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c msf2blc.c
+
+ckdd.o: ckdd.c array.h
+ $(CC) $(CFLAGS) -c ckdd.c
+
+alsnum.o: alsnum.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) -c alsnum.c
--- /dev/null
+# Makefile for ALSCRIPT, msf2blc and clus2blc programs
+#
+# These programs require an ANSI C compiler
+#
+# This makefile is for WATCOM C on a 386 or better PC.
+# Use the command wmake/u to build the alscript programs.
+#
+CC = wcc386
+CFLAGS =
+LFLAGS = /l=dos4g
+ALOBJS = alscript.obj alps.obj gjutil.obj agetbloc.obj ckdd.obj gjtimes.obj
+#
+PROGS = alscript.exe msf2blc.exe clus2blc.exe alsnum.exe fast2blc.exe
+
+all: $(PROGS)
+
+alscript.exe: $(ALOBJS)
+ $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) /fe=alscript.exe
+msf2blc.exe: msf2blc.obj gjutil.obj gjtimes.obj
+ $(CC) $(CFLAGS) msf2blc.obj gjutil.obj gjtimes.obj $(LFLAGS) /fe=msf2blc.exe
+fast2blc.exe: fast2blc.obj gjutil.obj gjtimes.obj
+ $(CC) $(CFLAGS) msf2blc.obj gjutil.obj gjtimes.obj $(LFLAGS) /fe=msf2blc.exe
+clus2blc.exe: clus2blc.obj gjutil.obj gjtimes.obj
+ $(CC) $(CFLAGS) clus2blc.obj gjutil.obj gjtimes.obj $(LFLAGS) /fe=clus2blc.exe
+alsnum.exe: alsnum.obj gjutil.obj agetbloc.obj gjtimes.obj
+ $(CC) $(CFLAGS) alsnum.obj gjutil.obj agetbloc.obj gjtimes.obj $(LFLAGS) /fe=alsnum.exe
+
+alscript.obj: alscript.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) alscript.c
+
+alps.obj: alps.c array.h gjutil.h version.h
+ $(CC) $(CFLAGS) alps.c
+
+agetbloc.obj: agetbloc.c array.h gjutil.h
+ $(CC) $(CFLAGS) agetbloc.c
+
+gjutil.obj: gjutil.c gjutil.h gjtimes.obj
+ $(CC) $(CFLAGS) gjutil.c gjtimes.obj
+
+gjtimes.obj: gjtimes.c gjtimes.h
+ $(CC) $(CFLAGS) gjtimes.c
+
+clus2blc.obj: clus2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) clus2blc.c
+
+msf2blc.obj: msf2blc.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) msf2blc.c
+
+ckdd.obj: ckdd.c array.h
+ $(CC) $(CFLAGS) ckdd.c
+
+alsnum.obj: alsnum.c array.h gjutil.h defaults.h
+ $(CC) $(CFLAGS) alsnum.c
+
+
+
+
+
+
+
--- /dev/null
+/***************************************************************************
+
+msf2blc: A program to convert a GCG .MSF file into an AMPS blockfile.
+
+ Copyright: Geoffrey J. Barton (1992,1997)
+
+ email: geoff@ebi.ac.uk
+ Please see the README file for details of conditions for use of this program.
+
+ $Id: msf2blc.c,v 1.3 1999/07/09 10:35:29 geoff Exp $
+ $Log: msf2blc.c,v $
+ Revision 1.3 1999/07/09 10:35:29 geoff
+ Change version and copyright statement to reflect 1997 status
+
+ Revision 1.2 1998/09/17 16:55:06 geoff
+ Check consistency with archive
+
+
+****************************************************************************
+
+Notes: This program can be run as a pipe: type msf2blc -q < input > output
+Only error messages will be output to std_err
+
+Default mode is interactive and prompts for filenames.
+
+The storage for the sequences is allocated dynamically, so the MAX_SEQ_LEN
+defines in the header file "defaults.h" have no effect. If a system memory
+limit is reached, then a "malloc error" message will be written and the
+program will stop. Most computers should happily cope with large numbers of
+long sequences. Some possible solutions to this problem are outlined in
+the user manual - alscript.doc
+
+24 October 1994: Add -n option to delete . from alignment if found.
+
+****************************************************************************/
+
+#include <stdio.h>
+#include <stdarg.h>
+#include <stdlib.h>
+#include <string.h>
+#include <ctype.h>
+
+#include "gjutil.h"
+#include "array.h"
+#include "defaults.h"
+
+#define TOKENS " \t\n"
+
+
+main(int argc,char *argv[])
+{
+ struct seqdat *seqs;
+ FILE *fp,*fout;
+ int nseq;
+ int found;
+ int i,j;
+ char *token,*sbit;
+ char *line;
+ extern FILE *std_err,*std_in,*std_out;
+ char *msffile;
+ char *blocfile;
+ int quiet;
+ int nodot;
+/*
+ std_err = stderr;
+ std_in = stdin;
+ std_out = stdout;
+*/
+
+ GJinitfile();
+
+ line = GJstrcreate(MAX_INLEN," ");
+ msffile = GJstrcreate(MAX_INLEN,NULL);
+ blocfile = GJstrcreate(MAX_INLEN,NULL);
+
+ nseq = 0;
+ found = 0;
+ quiet = 0;
+ nodot = 0;
+
+ if(argc > 1){
+ if(strcmp(argv[1],"-q")==0){
+ /* Quiet mode - read .MSF file from stdin and output block file to stdout */
+ quiet = 1;
+ fp = std_in;
+ fout = std_out;
+ }
+ if(argc > 2){
+ if(strcmp(argv[2],"-n")==0){
+ /* set flag to remove dots */
+ nodot = 1;
+ }
+ }
+ }else{
+ /* Verbose mode - prompt for all filenames */
+ fprintf(std_out,"\n\n");
+ fprintf(std_out,"GCG .MSF to AMPS Blockfile conversion\n");
+ fprintf(std_out,"Copyright: G. J. Barton (1992)\n");
+ fprintf(std_out,"Author: G. J. Barton (1992)\n\n");
+ fprintf(std_out,"Max number/length of alignment - Defined by System\n");
+ fprintf(std_out,"If you get a malloc error message - see manual\n\n");
+ fprintf(std_out,"Enter MSF filename: ");
+
+ fscanf(std_in,"%s",msffile);
+ fprintf(std_out,"Opening: %s\n",msffile);
+ fp = GJfopen(msffile,"r",1);
+
+ fprintf(std_out,"Enter Block filename: ");
+ fscanf(std_in,"%s",blocfile);
+ fprintf(std_out,"Opening: %s\n",blocfile);
+ fout = GJfopen(blocfile,"w",1);
+ }
+
+ fprintf(fout,"\n");
+ fprintf(fout,"Conversion of GCG .MSF file to AMPS BLOCKFILE format\n");
+ fprintf(fout,"msf2blc: Geoffrey J. Barton (1992)\n\n");
+
+ seqs = (struct seqdat *) GJmalloc(sizeof(struct seqdat));
+
+ if(!quiet)fprintf(std_out,"Reading .msf file\n");
+ while(fgets(line,MAX_INLEN,fp) != NULL){
+ if(line[0] != '\n'){
+ token = strtok(line,TOKENS);
+ if(token != NULL){
+ if(strcmp(token,"Name:") == 0){
+ /* This is a seq id name */
+ token = strtok(NULL,TOKENS);
+ seqs = (struct seqdat *) GJrealloc(seqs,sizeof(struct seqdat) * (nseq +1));
+ seqs[nseq].id = GJstrdup(token);
+ seqs[nseq].title = GJstrdup(line);
+ seqs[nseq].slen = 0;
+ seqs[nseq].seq = (char *) GJmalloc(sizeof(char));
+ ++nseq;
+ if(!quiet)fprintf(std_out,"%s\n",seqs[nseq-1].id);
+ }else if((strcmp(token,"//") == 0) || found){
+ /* this signals the end of identifiers so process sequences*/
+ found = 1;
+ if(token != NULL){
+ /* find out which seq this is */
+ i=0;
+ for(i=0;i<nseq;++i){
+ if(strcmp(token,seqs[i].id) == 0){
+ break;
+ }
+ }
+ /* read in the sequence */
+ if(i < nseq){
+ token = strtok(NULL,"\n");
+ if(token == NULL){
+ GJerror("Cannot find sequence in line");
+ fprintf(std_err,"%s",line);
+ exit(1);
+ }
+ j=0;
+ while(token[j] != '\0'){
+ if(isalpha(token[j]) || token[j] == '.'){
+ seqs[i].seq = (char *) GJrealloc(seqs[i].seq,sizeof(char) * (seqs[i].slen +1));
+ seqs[i].seq[seqs[i].slen] = token[j];
+ ++seqs[i].slen;
+ }
+ ++j;
+ }
+ }
+ }
+ }else{
+ /* this is a comment line - just echo */
+ fprintf(fout,"%s\n",line);
+ }
+ }
+ }
+ }
+ if(!quiet)fprintf(std_out,"All %d sequences read in\n",nseq);
+ if(!quiet)fprintf(std_out,"Writing .blc file\n");
+
+ for(i=0;i<nseq;++i){
+ fprintf(fout,">%s %s\n",seqs[i].id,seqs[i].title);
+ }
+ fprintf(fout,"* iteration 1\n");
+ for(i=0;i<seqs[0].slen;++i){
+ for(j=0;j<nseq;++j){
+ /* edit out dots if required */
+ if(nodot == 1){
+ if(seqs[j].seq[i] == '.'){
+ seqs[j].seq[i] = ' ';
+ }
+ }
+ fprintf(fout,"%c",seqs[j].seq[i]);
+ }
+ fprintf(fout,"\n");
+ }
+ fprintf(fout,"*\n");
+ if(!quiet)fprintf(std_out,"All done\n");
+
+ for(i=0;i<nseq;++i){
+ GJfree(seqs[i].seq);
+ GJfree(seqs[i].id);
+ GJfree(seqs[i].title);
+ }
+ GJfree(seqs);
+ GJfree(line);
+ GJfree(blocfile);
+ GJfree(msffile);
+
+}
--- /dev/null
+#include <stdio.h>
+#include <stdlib.h>
+#include <string.h>
+#include <gjutil.h>
+
+int GJindex(char *str,char c);
+
+float mzcons(unsigned char *pos,int n)
+
+/* calculate conservation value according to Zvelebil et al for a set
+ of amino acids and gaps stored in string pos of length n
+
+ Copyright: Geoffrey J. Barton (1992,1997)
+
+ email: geoff@ebi.ac.uk
+ Please see the README file for details of conditions for use of this program.
+
+ $Id: mzcons.c,v 1.2 1998/09/17 16:55:07 geoff Exp $
+ $Log: mzcons.c,v $
+ Revision 1.2 1998/09/17 16:55:07 geoff
+ Check consistency with archive
+
+
+*/
+
+{
+
+/* -- conmat contains a table like that shown in the JMB paper
+ order of amino acids is GAP,ARNDCQEGHILKMFPSTWYBZX
+*/
+ static char *acids = " ARNDCQEGHILKMFPSTWYBZX";
+ static int conmat[24][10] = {
+ {1,1,1,1,1,1,1,1,1,1},
+ {1,0,0,0,0,1,1,0,0,0},
+ {0,1,0,1,1,0,0,0,0,0},
+ {0,0,0,1,0,1,0,0,0,0},
+ {0,0,1,1,1,1,0,0,0,0},
+ {1,0,0,0,0,1,0,0,0,0},
+ {0,0,0,1,0,0,0,0,0,0},
+ {0,0,1,1,1,0,0,0,0,0},
+ {1,0,0,0,0,1,1,0,0,0},
+ {1,1,0,1,1,0,0,0,1,0},
+ {1,0,0,0,0,0,0,1,0,0},
+ {1,0,0,0,0,0,0,1,0,0},
+ {1,1,0,1,1,0,0,0,0,0},
+ {1,0,0,0,0,0,0,0,0,0},
+ {1,0,0,0,0,0,0,0,1,0},
+ {0,0,0,0,0,1,0,0,0,1},
+ {0,0,0,1,0,1,1,0,0,0},
+ {1,0,0,1,0,1,0,0,0,0},
+ {1,0,0,1,0,0,0,0,1,0},
+ {1,0,0,1,0,0,0,0,1,0},
+ {1,0,0,0,0,1,0,1,0,0},
+ {0,0,0,1,0,0,0,0,0,0},
+ {0,0,0,1,0,0,0,0,0,0},
+ {1,1,1,1,1,1,1,1,1,1}
+ };
+
+ int concnt,it,i,j,k,l,tcnt,ibseq;
+ int nide, facid;
+
+ concnt = 0;
+ for(j=0;j<10;++j){
+ /*found: outer loop over properties
+ set IT to the value of the first amino acid*/
+ it = conmat[GJindex(acids,pos[0])][j];
+ /*loop over remaining acids, if a difference occurs then
+ set tcnt to 1*/
+ tcnt = 0;
+ for(k=1;k<n;++k){
+ if(it != conmat[GJindex(acids,pos[k])][j]){
+ tcnt = 1;
+ }
+ }
+ /*add tcnt to concnt*/
+ concnt += tcnt;
+ }
+ /* check for total identity at this position*/
+ facid = pos[0];
+ nide = 0;
+ for(k=1;k<n;++k){
+ if(facid == pos[k]){
+ ++nide;
+ }
+ }
+
+ /*calculate the conservation*/
+ if(concnt == 10){
+ con = 0.0;
+ }else if(concnt == 0){
+ if(nide == n){
+ /*total identity*/
+ con = 1.0;
+ }else{
+ /*not identity, but same properties*/
+ con = 0.9;
+ }
+ }else{
+ con = 0.9 - 0.1 * concnt;
+ }
+ return con;
+}
+
+
+int GJindex(char *str,char c)
+{
+ char *t;
+ t = strchr(str,c);
+ if(t == NULL) return NULL;
+ return (int) (t - str);
+}
+
+
+
+
+
+
+
+
+
+
+
--- /dev/null
+
+#define ALSCRIPT_VERSION "ALSCRIPT Version 2.07 - 9 July 1999"
+
+/*
+ $Id: version.h,v 1.4 1999/07/09 10:35:30 geoff Exp $
+ $Log: version.h,v $
+ Revision 1.4 1999/07/09 10:35:30 geoff
+ Change version and copyright statement to reflect 1997 status
+
+ Revision 1.3 1998/09/17 17:38:40 geoff
+ Update version details
+
+ Revision 1.2 1998/09/17 16:55:07 geoff
+ Check consistency with archive
+
+*/
git://source.jalview.org / jpred.git / commitdiff