2 package "RNAinverse" # don't use package if you're using automake
4 purpose "Find RNA sequences with given secondary structure"
6 #usage "RNAinverse [options]\n"
8 # Version of your program
9 #version "2.0" # don't use version if you're using automake
12 # command line options passed to gengetopt
13 args "--file-name=RNAinverse_cmdl --include-getopt --default-optional --func-name=RNAinverse_cmdline_parser --arg-struct-name=RNAinverse_args_info"
16 description "The program searches for sequences folding into a predefined structure, thereby\
17 inverting the folding algorithm. Target structures (in bracket notation) and starting sequences\
18 for the search are read alternately from stdin.\nCharacters in the start sequence other than \"AUGC\"\
19 (or the alphabet specified with -a) will be treated as wild cards and replaced by a random character.\
20 Any lower case characters in the start sequence will be kept fixed during the search. If necessary,\
21 the sequence will be elongated to the length of the structure. Thus a string of \"N\"s as well as a\
22 blank line specify a random start sequence.\nFor each search the best sequence found and its Hamming\
23 distance to the start sequence are printed to stdout. If the the search was unsuccessful, a structure\
24 distance to the target is appended.\nThe -Fp and -R options can modify the output format, see commandline\
25 options below.\nThe program will continue to read new structures and sequences until a line consisting\
26 of the single character \"@\" or an end of file condition is encountered.\n"
29 section "General Options"
30 sectiondesc="Below are command line options which alter the general behavior of this program\n"
33 "Search repeatedly for the same structure.\nIf an argument is supplied to this option it must\
34 follow the option flag immediately. E.g.: -R5\n"
35 details="If repeats is negative search until --repeats exact solutions are found, no\
36 output is done for unsuccessful searches. Be aware, that the program will not terminate\
37 if the target structure can not be found.\nIf no value is supplied with this option, the\
38 default value is used.\n\n"
45 "Find sequences using only nucleotides from a given alphabet.\n\n"
51 "In conjunction with a negative value supplied to -R, print the last subsequence and\
52 substructure for each unsuccessful search.\n\n"
57 sectiondesc="Select additional algorithms which should be included in the calculations.\n\n"
60 "Use minimum energy (-Fm), partition function folding (-Fp) or both (-Fmp).\n"
61 details="In partition function mode, the probability of the target structure exp(-E(S)/kT)/Q is maximized.\
62 This probability is written in brackets after the found sequence and Hamming distance. In most cases\
63 you'll want to use the -f option in conjunction with -Fp, see below.\n\n"
70 "In combination with -Fp stop search when sequence is found with E(s)-F is smaller\
71 than final, where F=-kT*ln(Q).\n\n"
75 section "Model Details"
78 "Rescale energy parameters to a temperature of temp C. Default is 37C.\n\n"
82 "Do not include special tabulated stabilizing energies for tri-, tetra- and hexaloop hairpins. Mostly for testing.\n\n"
87 "How to treat \"dangling end\" energies for bases adjacent to helices in free ends and multi-loops\n"
88 details="\nWith -d1 only unpaired bases can participate in at most one dangling end, this is unsupported\
89 for the partition function folding.\n\nWith -d2 this check is ignored,\
90 dangling energies will be added for the bases adjacent to a helix on both sides in any case; this is the\
91 default for partition function folding (-p).\nThe option -d0 ignores dangling ends altogether (mostly for\
92 debugging).\nWith -d3 mfe folding will allow coaxial stacking of adjacent helices in multi-loops. At the\
93 moment the implementation will not allow coaxial stacking of the two interior pairs in a loop of degree 3\
94 and works only for mfe folding.\n\nNote that by default (as well as with -d1 and -d3) pf and mfe folding\
95 treat dangling ends differently. Use -d2 in addition to -Fp to ensure that both algorithms use the same\
102 "Do not allow GU pairs\n\n"
106 option "noClosingGU" -
107 "Do not allow GU pairs at the end of helices\n\n"
112 "Read energy parameters from paramfile, instead of using the default parameter set.\n"
113 details="A sample parameter file should accompany your distribution.\nSee the RNAlib\
114 documentation for details on the file format.\n\n"
120 "Allow other pairs in addition to the usual AU,GC,and GU pairs.\n"
121 details="Its argument is a comma separated list of additionally allowed pairs. If the\
122 first character is a \"-\" then AB will imply that AB and BA are allowed pairs.\ne.g.\
123 RNAfold -nsp -GA will allow GA and AG pairs. Nonstandard pairs are given 0 stacking\
129 option "energyModel" e
130 "Rarely used option to fold sequences from the artificial ABCD... alphabet, where\
131 A pairs B, C-D etc. Use the energy parameters for GC (-e 1) or AU (-e 2) pairs.\n\n"
136 text "\nIf in doubt our program is right, nature is at fault.\nComments should be sent to\
137 rna@tbi.univie.ac.at.\n"