October 6, 2006

The FASTA programs for Windows32 environments (WindowsNT, 2000, XP)
has undergone a major upgrade, so that now all the programs in the
Unix/MacOSX distribution are available to Windows users.  Moreover,
Windows users with modern (SSE2 compatible) processors can run greatly
accelerated versions of the Smith-Waterman ssearch program.

Moreover, these programs work both with FASTA formatted files, and
NCBI BLAST formatted files.

The following programs are available:

    fasta34.exe	       	protein-protein or DNA-DNA database searches
    fastf34.exe
    fastm34.exe
    fasts34.exe
    fastx34.exe		compare DNA query to protein library with frameshifts
    fasty34.exe		compare DNA query to protein library with frameshifts
    prfx34.exe
    prss34.exe		evaluate statistical significance using shuffles
    prss34sse2.exe
    ssearch34.exe	Smith-Waterman for prot-prot or DNA-DNA searches
    ssearch34sse2.exe	Smith-Waterman, accelerated with SSE2 extensions
    tfastf34.exe
    tfastm34.exe
    tfasts34.exe
    tfastx34.exe	compare protein to DNA library with frameshifts
    tfasty34.exe	compare protein to DNA library with frameshifts

Each of these programs also has a "threaded" version, which can run on
multiple processors (or dual cores) if they are available.  However,
they are built using the Unix pthreads API, so to use these programs,
you must download the pthreadVC2.dll from:

ftp://sources.redhat.com/pub/pthreads-win32/dll-latest/lib/pthreadVC2.dll

see also http://sourceware.org/pthreads-win32/

    fasta34_t.exe
    fastf34_t.exe
    fastm34_t.exe
    fasts34_t.exe
    fastx34_t.exe
    fasty34_t.exe
    prfx34_t.exe
    prss34_t.exe
    prss34sse2_t.exe
    ssearch34_t.exe
    ssearch34sse2_t.exe
    tfastf34_t.exe
    tfasts34_t.exe
    tfastx34_t.exe
    tfasty34_t.exe

Without that DLL, the threaded programs will not run at all. The
current compilation supports two threads, and speeds up searches about
2-fold on dual-core processors.

The programs have been tested with protein and DNA databases in FASTA
format, PIR/GCG-text format, and Genbank flatfile format.  The program
does not work properly with GCG binary format databases, but it seems
unlikely that Windows users would need these. 

Be certain to use an program that can work with long file names when
unpacking the program source files.

Please report bugs to:

	wrp@virginia.edu