package org.forester.application; import java.io.File; import java.io.FileInputStream; import java.util.ArrayList; import java.util.List; import org.forester.io.parsers.FastaParser; import org.forester.io.parsers.GeneralMsaParser; import org.forester.msa.Msa; import org.forester.msa.MsaCompactor; import org.forester.msa.MsaMethods; import org.forester.util.CommandLineArguments; import org.forester.util.ForesterUtil; public class msa_compactor { final static private String HELP_OPTION_1 = "help"; final static private String HELP_OPTION_2 = "h"; final static private String REMOVE_WORST_OFFENDERS_OPTION = "w"; final static private String AV_GAPINESS_OPTION = "a"; final static private String STEP_OPTION = "s"; final static private String LENGTH_OPTION = "l"; final static private String REALIGN_OPTION = "r"; final static private String PRG_NAME = "msa_compactor"; final static private String PRG_DESC = "multiple sequnce aligment compactor"; final static private String PRG_VERSION = "0.90"; final static private String PRG_DATE = "2012.07.13"; final static private String E_MAIL = "phylosoft@gmail.com"; final static private String WWW = "www.phylosoft.org/forester/"; public static void main( final String args[] ) { try { final CommandLineArguments cla = new CommandLineArguments( args ); if ( cla.isOptionSet( HELP_OPTION_1 ) || cla.isOptionSet( HELP_OPTION_2 ) || ( cla.getNumberOfNames() != 2 ) ) { printHelp(); System.exit( 0 ); } final File in = cla.getFile( 0 ); final File out = cla.getFile( 1 ); int worst_remove = -1; double av = -1; int length = -1; int step = 1; boolean realign = false; final List allowed_options = new ArrayList(); allowed_options.add( REMOVE_WORST_OFFENDERS_OPTION ); allowed_options.add( AV_GAPINESS_OPTION ); allowed_options.add( LENGTH_OPTION ); allowed_options.add( REALIGN_OPTION ); allowed_options.add( STEP_OPTION ); final String dissallowed_options = cla.validateAllowedOptionsAsString( allowed_options ); if ( dissallowed_options.length() > 0 ) { ForesterUtil.fatalError( PRG_NAME, "unknown option(s): " + dissallowed_options ); } if ( cla.isOptionSet( REMOVE_WORST_OFFENDERS_OPTION ) ) { worst_remove = cla.getOptionValueAsInt( REMOVE_WORST_OFFENDERS_OPTION ); } if ( cla.isOptionSet( AV_GAPINESS_OPTION ) ) { av = cla.getOptionValueAsDouble( AV_GAPINESS_OPTION ); } if ( cla.isOptionSet( LENGTH_OPTION ) ) { length = cla.getOptionValueAsInt( LENGTH_OPTION ); } if ( cla.isOptionSet( STEP_OPTION ) ) { step = cla.getOptionValueAsInt( STEP_OPTION ); } if ( cla.isOptionSet( REALIGN_OPTION ) ) { realign = true; } // else if ( cla.isOptionSet( STEP_OPTION ) && cla.isOptionSet( WINDOW_OPTION ) ) { // step = cla.getOptionValueAsInt( STEP_OPTION ); // window = cla.getOptionValueAsInt( WINDOW_OPTION ); // } // else { // printHelp(); // System.exit( 0 ); // } Msa msa = null; final FileInputStream is = new FileInputStream( in ); if ( FastaParser.isLikelyFasta( in ) ) { msa = FastaParser.parseMsa( is ); } else { msa = GeneralMsaParser.parse( is ); } MsaCompactor mc = null; if ( worst_remove > 0 ) { mc = MsaCompactor.removeWorstOffenders( msa, worst_remove, realign ); } else if ( av > 0 ) { mc = MsaCompactor.reduceGapAverage( msa, av, step, realign, out, 50 ); } else if ( length > 0 ) { mc = MsaCompactor.reduceLength( msa, length, step, realign ); } System.out.println( MsaMethods.calcGapRatio( mc.getMsa() ) ); for( final String id : mc.getRemovedSeqIds() ) { System.out.println( id ); } } catch ( final Exception e ) { e.printStackTrace(); ForesterUtil.fatalError( PRG_NAME, e.getMessage() ); } } private static void printHelp() { ForesterUtil.printProgramInformation( PRG_NAME, PRG_DESC, PRG_VERSION, PRG_DATE, E_MAIL, WWW, ForesterUtil.getForesterLibraryInformation() ); System.out.println( "Usage:" ); System.out.println(); System.out.println( PRG_NAME + " " ); System.out.println(); System.out.println( " options: " ); System.out.println(); // System.out.println( " -" + FROM_OPTION + "=: from (msa column)" ); // System.out.println( " -" + TO_OPTION + "=: to (msa column)" ); // System.out.println( " or" ); // System.out.println( " -" + WINDOW_OPTION + "=: window size (msa columns)" ); System.out.println( " -" + REMOVE_WORST_OFFENDERS_OPTION + "=: step size (msa columns)" ); System.out.println(); System.out.println(); System.out.println(); } }