// $Id: // FORESTER -- software libraries and applications // for evolutionary biology research and applications. // // Copyright (C) 2008-2009 Christian M. Zmasek // Copyright (C) 2008-2009 Burnham Institute for Medical Research // All rights reserved // // This library is free software; you can redistribute it and/or // modify it under the terms of the GNU Lesser General Public // License as published by the Free Software Foundation; either // version 2.1 of the License, or (at your option) any later version. // // This library is distributed in the hope that it will be useful, // but WITHOUT ANY WARRANTY; without even the implied warranty of // MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU // Lesser General Public License for more details. // // You should have received a copy of the GNU Lesser General Public // License along with this library; if not, write to the Free Software // Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA // // Contact: phylosoft @ gmail . com // WWW: www.phylosoft.org/forester package org.forester.application; import java.io.File; import java.io.IOException; import java.util.ArrayList; import java.util.Date; import java.util.List; import org.forester.io.parsers.phyloxml.PhyloXmlParser; import org.forester.io.writers.PhylogenyWriter; import org.forester.phylogeny.Phylogeny; import org.forester.phylogeny.factories.ParserBasedPhylogenyFactory; import org.forester.phylogeny.factories.PhylogenyFactory; import org.forester.sdi.GSDI; import org.forester.sdi.SDI; import org.forester.sdi.SDIse; import org.forester.util.CommandLineArguments; import org.forester.util.ForesterUtil; public final class sdi { final static private String STRIP_OPTION = "s"; final static private String GSDI_OPTION = "g"; final static private String MOST_PARSIMONIOUS_OPTION = "m"; final static private String HELP_OPTION_1 = "help"; final static private String HELP_OPTION_2 = "h"; final static private String DEFAULT_OUTFILE = "sdi_out.xml"; final static private String PRG_NAME = "sdi"; final static private String PRG_VERSION = "beta 0.4"; final static private String PRG_DATE = "2009.01.22"; public static void main( final String args[] ) { ForesterUtil.printProgramInformation( sdi.PRG_NAME, sdi.PRG_VERSION, sdi.PRG_DATE ); CommandLineArguments cla = null; try { cla = new CommandLineArguments( args ); } catch ( final Exception e ) { ForesterUtil.fatalError( PRG_NAME, e.getMessage() ); } if ( cla.isOptionSet( sdi.HELP_OPTION_1 ) || cla.isOptionSet( sdi.HELP_OPTION_2 ) ) { System.out.println(); sdi.print_help(); System.exit( 0 ); } else if ( ( args.length < 2 ) || ( cla.getNumberOfNames() < 2 ) || ( cla.getNumberOfNames() > 3 ) ) { System.out.println(); System.out.println( "Wrong number of arguments." ); System.out.println(); sdi.print_help(); System.exit( -1 ); } final List allowed_options = new ArrayList(); allowed_options.add( sdi.STRIP_OPTION ); allowed_options.add( sdi.GSDI_OPTION ); allowed_options.add( sdi.MOST_PARSIMONIOUS_OPTION ); final String dissallowed_options = cla.validateAllowedOptionsAsString( allowed_options ); if ( dissallowed_options.length() > 0 ) { ForesterUtil.fatalError( sdi.PRG_NAME, "unknown option(s): " + dissallowed_options ); } boolean use_sdise = true; boolean strip = false; boolean most_parsimonous_duplication_model = false; if ( cla.isOptionSet( sdi.STRIP_OPTION ) ) { strip = true; } if ( cla.isOptionSet( sdi.GSDI_OPTION ) ) { use_sdise = false; } if ( cla.isOptionSet( sdi.MOST_PARSIMONIOUS_OPTION ) ) { if ( use_sdise ) { ForesterUtil.fatalError( sdi.PRG_NAME, "Can only use most parsimonious duplication mode with GSDI" ); } most_parsimonous_duplication_model = true; } Phylogeny species_tree = null; Phylogeny gene_tree = null; File gene_tree_file = null; File species_tree_file = null; File out_file = null; try { gene_tree_file = cla.getFile( 0 ); species_tree_file = cla.getFile( 1 ); if ( cla.getNumberOfNames() == 3 ) { out_file = cla.getFile( 2 ); } else { out_file = new File( sdi.DEFAULT_OUTFILE ); } } catch ( final IllegalArgumentException e ) { ForesterUtil.fatalError( sdi.PRG_NAME, "error in command line: " + e.getMessage() ); } if ( ForesterUtil.isReadableFile( gene_tree_file ) != null ) { ForesterUtil.fatalError( sdi.PRG_NAME, ForesterUtil.isReadableFile( gene_tree_file ) ); } if ( ForesterUtil.isReadableFile( species_tree_file ) != null ) { ForesterUtil.fatalError( sdi.PRG_NAME, ForesterUtil.isReadableFile( species_tree_file ) ); } if ( ForesterUtil.isWritableFile( out_file ) != null ) { ForesterUtil.fatalError( sdi.PRG_NAME, ForesterUtil.isWritableFile( out_file ) ); } try { final PhylogenyFactory factory = ParserBasedPhylogenyFactory.getInstance(); species_tree = factory.create( species_tree_file, new PhyloXmlParser() )[ 0 ]; } catch ( final IOException e ) { ForesterUtil.fatalError( sdi.PRG_NAME, "Failed to read species tree from \"" + gene_tree_file + "\" [" + e.getMessage() + "]" ); } try { final PhylogenyFactory factory = ParserBasedPhylogenyFactory.getInstance(); gene_tree = factory.create( gene_tree_file, new PhyloXmlParser() )[ 0 ]; } catch ( final IOException e ) { ForesterUtil.fatalError( sdi.PRG_NAME, "Failed to read gene tree from \"" + gene_tree_file + "\" [" + e.getMessage() + "]" ); } gene_tree.setRooted( true ); species_tree.setRooted( true ); if ( !gene_tree.isCompletelyBinary() ) { ForesterUtil.fatalError( sdi.PRG_NAME, "gene tree (\"" + gene_tree_file + "\") is not completely binary." ); } if ( use_sdise ) { if ( !species_tree.isCompletelyBinary() ) { ForesterUtil.fatalError( sdi.PRG_NAME, "species tree (\"" + species_tree_file + "\") is not completely binary." ); } } // For timing. // gene_tree = Helper.createBalancedTree( 10 ); // species_tree = Helper.createBalancedTree( 13 ); // species_tree = Helper.createUnbalancedTree( 1024 ); // gene_tree = Helper.createUnbalancedTree( 8192 ); // species_tree = gene_tree.copyTree(); // gene_tree = species_tree.copyTree(); // Helper.numberSpeciesInOrder( species_tree ); // Helper.numberSpeciesInOrder( gene_tree ); // Helper.randomizeSpecies( 1, 8192, gene_tree ); // Helper.intervalNumberSpecies( gene_tree, 4096 ); // Helper.numberSpeciesInDescOrder( gene_tree ); System.out.println(); System.out.println( "Strip species tree: " + strip ); SDI sdi = null; final long start_time = new Date().getTime(); try { if ( use_sdise ) { System.out.println(); System.out.println( "Using SDIse algorithm." ); sdi = new SDIse( gene_tree, species_tree ); } else { System.out.println(); System.out.println( "Using GSDI algorithm." ); System.out.println(); System.out.println( "Use most parsimonous duplication model: " + most_parsimonous_duplication_model ); sdi = new GSDI( gene_tree, species_tree, most_parsimonous_duplication_model ); } } catch ( final Exception e ) { ForesterUtil.fatalError( PRG_NAME, e.getLocalizedMessage() ); } System.out.println(); System.out.println( "Running time (excluding I/O): " + ( new Date().getTime() - start_time ) + "ms" ); try { final PhylogenyWriter writer = new PhylogenyWriter(); writer.toPhyloXML( out_file, gene_tree, 1 ); } catch ( final IOException e ) { ForesterUtil.fatalError( PRG_NAME, "Failed to write to \"" + out_file + "\" [" + e.getMessage() + "]" ); } System.out.println(); System.out.println( "Successfully wrote resulting gene tree to: " + out_file ); System.out.println(); if ( use_sdise ) { sdi.computeMappingCostL(); System.out.println( "Mapping cost : " + sdi.computeMappingCostL() ); } System.out.println( "Number of duplications : " + sdi.getDuplicationsSum() ); if ( !use_sdise && !most_parsimonous_duplication_model ) { System.out.println( "Number of potential duplications: " + ( ( GSDI ) sdi ).getSpeciationOrDuplicationEventsSum() ); } if ( !use_sdise ) { System.out.println( "Number speciations : " + ( ( GSDI ) sdi ).getSpeciationsSum() ); } System.out.println(); } // main( final String args[] ) private static void print_help() { System.out.println( "Usage: \"" + sdi.PRG_NAME + " [-options] [outfile]\"" ); System.out.println(); System.out.println( "Options:" ); System.out.println( " -" + sdi.STRIP_OPTION + ": to strip the species tree prior to duplication inference" ); System.out.println( " -" + sdi.GSDI_OPTION + ": to use GSDI algorithm instead of SDIse algorithm (under development, not recommended)" ); System.out .println( " -" + sdi.MOST_PARSIMONIOUS_OPTION + ": use most parimonious duplication model for GSDI: " ); System.out.println( " assign nodes as speciations which would otherwise be assiged" ); System.out.println( " as unknown because of polytomies in the species tree" ); System.out.println(); System.out.println( "Species tree:" ); System.out.println( " In phyloXML format, with taxonomy data in appropriate fields." ); System.out.println(); System.out.println( "Gene tree:" ); System.out.println( " In phyloXM format, with taxonomy and sequence data in appropriate fields." ); System.out.println(); System.out .println( "!! WARNING: GSDI algorithm is under development (and possibly not correct), please use SDIse instead !!" ); System.out.println(); } }