// $Id: // // forester -- software libraries and applications // for genomics and evolutionary biology research. // // Copyright (C) 2010 Christian M Zmasek // Copyright (C) 2010 Sanford-Burnham Medical Research Institute // All rights reserved // // This library is free software; you can redistribute it and/or // modify it under the terms of the GNU Lesser General Public // License as published by the Free Software Foundation; either // version 2.1 of the License, or (at your option) any later version. // // This library is distributed in the hope that it will be useful, // but WITHOUT ANY WARRANTY; without even the implied warranty of // MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU // Lesser General Public License for more details. // // You should have received a copy of the GNU Lesser General Public // License along with this library; if not, write to the Free Software // Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA // // Contact: phylosoft @ gmail . com // WWW: https://sites.google.com/site/cmzmasek/home/software/forester package org.forester.io.parsers; import java.io.BufferedReader; import java.io.ByteArrayInputStream; import java.io.File; import java.io.FileInputStream; import java.io.IOException; import java.io.InputStream; import java.io.InputStreamReader; import java.util.ArrayList; import java.util.List; import java.util.regex.Matcher; import java.util.regex.Pattern; import org.forester.msa.BasicMsa; import org.forester.msa.Msa; import org.forester.msa.MsaFormatException; import org.forester.sequence.BasicSequence; import org.forester.sequence.MolecularSequence; public class FastaParser { private static final Pattern NAME_REGEX = Pattern.compile( "^\\s*>\\s*(.+)" ); private static final Pattern SEQ_REGEX = Pattern.compile( "^\\s*(.+)" ); private static final Pattern ANYTHING_REGEX = Pattern.compile( "[\\d\\s]+" ); //>gi|71834668|ref|NP_001025424.1| Bcl2 [Danio rerio] public static final Pattern FASTA_DESC_LINE = Pattern .compile( ">?\\s*([^|]+)\\|([^|]+)\\S*\\s+(.+)\\s+\\[(.+)\\]" ); public static void main( final String[] args ) { final String a = ">gi|71834668|ref|NP_001025424.1| Bcl2 [Danio rerio]"; final Matcher name_m = FASTA_DESC_LINE.matcher( a ); if ( name_m.lookingAt() ) { System.out.println(); System.out.println( name_m.group( 1 ) ); System.out.println( name_m.group( 2 ) ); System.out.println( name_m.group( 3 ) ); System.out.println( name_m.group( 4 ) ); } else { System.out.println( "Does not match." ); } } static public boolean isLikelyFasta( final File f ) throws IOException { return isLikelyFasta( new FileInputStream( f ) ); } static public boolean isLikelyFasta( final InputStream is ) throws IOException { final BufferedReader reader = new BufferedReader( new InputStreamReader( is, "UTF-8" ) ); String line = null; while ( ( line = reader.readLine() ) != null ) { final boolean is_name_line = NAME_REGEX.matcher( line ).lookingAt(); if ( canIgnore( line, true, false ) ) { continue; } else if ( is_name_line ) { reader.close(); return true; } else if ( SEQ_REGEX.matcher( line ).lookingAt() ) { reader.close(); return false; } } reader.close(); return false; } static public Msa parseMsa( final File f ) throws IOException { return parseMsa( new FileInputStream( f ) ); } static public Msa parseMsa( final InputStream is ) throws IOException { return BasicMsa.createInstance( parse( is ) ); } static public Msa parseMsa( final String s ) throws IOException { return parseMsa( s.getBytes() ); } static public Msa parseMsa( final byte[] bytes ) throws IOException { return parseMsa( new ByteArrayInputStream( bytes ) ); } static public List parse( final File f ) throws IOException { return parse( new FileInputStream( f ) ); } static public List parse( final InputStream is ) throws IOException { final BufferedReader reader = new BufferedReader( new InputStreamReader( is, "UTF-8" ) ); String line = null; int line_counter = 0; boolean saw_first_seq = false; StringBuilder current_seq = null; StringBuilder name = null; final List temp_msa = new ArrayList(); while ( ( line = reader.readLine() ) != null ) { ++line_counter; final Matcher name_m = NAME_REGEX.matcher( line ); final boolean is_name_line = name_m.lookingAt(); if ( canIgnore( line, saw_first_seq, is_name_line ) ) { continue; } final Matcher seq_m = SEQ_REGEX.matcher( line ); if ( is_name_line ) { saw_first_seq = true; addSeq( name, current_seq, temp_msa ); name = new StringBuilder( name_m.group( 1 ).trim() ); current_seq = new StringBuilder(); } else if ( seq_m.lookingAt() ) { if ( name.length() < 1 ) { reader.close(); throw new MsaFormatException( "illegally formatted fasta msa (line: " + line_counter + "):\n\"" + trim( line ) + "\"" ); } current_seq.append( seq_m.group( 1 ).replaceAll( "\\s+", "" ) ); } else { reader.close(); throw new MsaFormatException( "illegally formatted fasta msa (line: " + line_counter + "):\n\"" + trim( line ) + "\"" ); } } addSeq( name, current_seq, temp_msa ); reader.close(); final List seqs = new ArrayList(); for( int i = 0; i < temp_msa.size(); ++i ) { seqs.add( BasicSequence.createAaSequence( temp_msa.get( i )[ 0 ].toString(), temp_msa.get( i )[ 1 ].toString() ) ); } return seqs; } static private boolean canIgnore( final String line, final boolean saw_first_seq, final boolean is_name_line ) { if ( ( line.length() < 1 ) || ANYTHING_REGEX.matcher( line ).matches() ) { return true; } if ( !saw_first_seq && !is_name_line ) { return true; } return false; } private static void addSeq( final StringBuilder name, final StringBuilder seq, final List temp_msa ) { if ( ( name != null ) && ( seq != null ) && ( name.length() > 0 ) && ( seq.length() > 0 ) ) { final StringBuilder[] ary = new StringBuilder[ 2 ]; ary[ 0 ] = name; ary[ 1 ] = seq; temp_msa.add( ary ); } } private static String trim( final String line ) { if ( line.length() > 100 ) { return line.substring( 0, 100 ) + " ..."; } return line; } }