// $Id: // forester -- software libraries and applications // for genomics and evolutionary biology research. // // Copyright (C) 2010 Christian M Zmasek // Copyright (C) 2010 Sanford-Burnham Medical Research Institute // All rights reserved // // This library is free software; you can redistribute it and/or // modify it under the terms of the GNU Lesser General Public // License as published by the Free Software Foundation; either // version 2.1 of the License, or (at your option) any later version. // // This library is distributed in the hope that it will be useful, // but WITHOUT ANY WARRANTY; without even the implied warranty of // MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU // Lesser General Public License for more details. // // You should have received a copy of the GNU Lesser General Public // License along with this library; if not, write to the Free Software // Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA // // Contact: phylosoft @ gmail . com // WWW: https://sites.google.com/site/cmzmasek/home/software/forester package org.forester.msa; import java.io.BufferedWriter; import java.io.File; import java.io.FileWriter; import java.io.IOException; import java.util.ArrayList; import java.util.List; import org.forester.io.parsers.FastaParser; import org.forester.io.writers.SequenceWriter; import org.forester.io.writers.SequenceWriter.SEQ_FORMAT; import org.forester.sequence.MolecularSequence; import org.forester.util.SystemCommandExecutor; public final class Mafft extends MsaInferrer { private final static String DEFAULT_PARAMETERS = "--maxiterate 1000 --localpair"; private String _error; private int _exit_code; private final String _path_to_prg; public static MsaInferrer createInstance( final String path_to_prg ) throws IOException { return new Mafft( path_to_prg ); } private Mafft( final String path_to_prg ) throws IOException { if ( !isInstalled( path_to_prg ) ) { throw new IOException( "cannot execute MAFFT with \"" + path_to_prg + "\"" ); } _path_to_prg = new String( path_to_prg ); init(); } public static String getDefaultParameters() { return DEFAULT_PARAMETERS; } @Override public String getErrorDescription() { return _error; } @Override public int getExitCode() { return _exit_code; } @Override public Msa infer( final List seqs, final List opts ) throws IOException, InterruptedException { final File file = File.createTempFile( "__mafft_input_", ".fasta" ); file.deleteOnExit(); final BufferedWriter writer = new BufferedWriter( new FileWriter( file ) ); SequenceWriter.writeSeqs( seqs, writer, SEQ_FORMAT.FASTA, 100 ); writer.close(); final Msa msa = infer( file, opts ); file.delete(); return msa; } @Override public Msa infer( final File path_to_input_seqs, final List opts ) throws IOException, InterruptedException { init(); final List my_opts = new ArrayList(); my_opts.add( _path_to_prg ); for( int i = 0; i < opts.size(); i++ ) { my_opts.add( opts.get( i ) ); } my_opts.add( path_to_input_seqs.getAbsolutePath() ); final SystemCommandExecutor command_executor = new SystemCommandExecutor( my_opts ); final int _exit_code = command_executor.executeCommand(); final StringBuilder stderr = command_executor.getStandardErrorFromCommand(); _error = stderr.toString(); if ( _exit_code != 0 ) { throw new IOException( "MAFFT program failed, exit code: " + _exit_code + "\nCommand:\n" + my_opts + "\nError:\n" + stderr ); } final StringBuilder stdout = command_executor.getStandardOutputFromCommand(); if ( ( stdout == null ) || ( stdout.length() < 2 ) ) { throw new IOException( "MAFFT program did not produce any output\nCommand:\n" + my_opts + "\nError:\n" + stderr ); } final Msa msa = FastaParser.parseMsa( stdout.toString() ); return msa; } private void init() { _error = null; _exit_code = -100; } }