/*
* Jalview - A Sequence Alignment Editor and Viewer (Version 2.8)
* Copyright (C) 2012 J Procter, AM Waterhouse, LM Lui, J Engelhardt, G Barton, M Clamp, S Searle
*
* This file is part of Jalview.
*
* Jalview is free software: you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
*
* Jalview is distributed in the hope that it will be useful, but
* WITHOUT ANY WARRANTY; without even the implied warranty
* of MERCHANTABILITY or FITNESS FOR A PARTICULAR
* PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License along with Jalview. If not, see .
*/
package jalview.analysis;
import java.util.ArrayList;
import java.util.Hashtable;
import java.util.Vector;
import jalview.datamodel.AlignedCodonFrame;
import jalview.datamodel.Alignment;
import jalview.datamodel.AlignmentAnnotation;
import jalview.datamodel.AlignmentI;
import jalview.datamodel.Annotation;
import jalview.datamodel.DBRefEntry;
import jalview.datamodel.FeatureProperties;
import jalview.datamodel.Mapping;
import jalview.datamodel.Sequence;
import jalview.datamodel.SequenceFeature;
import jalview.datamodel.SequenceI;
import jalview.schemes.ResidueProperties;
import jalview.util.MapList;
import jalview.util.ShiftList;
public class Dna
{
/**
*
* @param cdp1
* @param cdp2
* @return -1 if cdp1 aligns before cdp2, 0 if in the same column or cdp2 is
* null, +1 if after cdp2
*/
private static int compare_codonpos(int[] cdp1, int[] cdp2)
{
if (cdp2 == null
|| (cdp1[0] == cdp2[0] && cdp1[1] == cdp2[1] && cdp1[2] == cdp2[2]))
return 0;
if (cdp1[0] < cdp2[0] || cdp1[1] < cdp2[1] || cdp1[2] < cdp2[2])
return -1; // one base in cdp1 precedes the corresponding base in the
// other codon
return 1; // one base in cdp1 appears after the corresponding base in the
// other codon.
}
/**
* DNA->mapped protein sequence alignment translation given set of sequences
* 1. id distinct coding regions within selected region for each sequence 2.
* generate peptides based on inframe (or given) translation or (optionally
* and where specified) out of frame translations (annotated appropriately) 3.
* align peptides based on codon alignment
*/
/**
* id potential products from dna 1. search for distinct products within
* selected region for each selected sequence 2. group by associated DB type.
* 3. return as form for input into above function
*/
/**
*
*/
/**
* create a new alignment of protein sequences by an inframe translation of
* the provided NA sequences
*
* @param selection
* @param seqstring
* @param viscontigs
* @param gapCharacter
* @param annotations
* @param aWidth
* @param dataset
* destination dataset for translated sequences and mappings
* @return
*/
public static AlignmentI CdnaTranslate(SequenceI[] selection,
String[] seqstring, int viscontigs[], char gapCharacter,
AlignmentAnnotation[] annotations, int aWidth, Alignment dataset)
{
return CdnaTranslate(selection, seqstring, null, viscontigs,
gapCharacter, annotations, aWidth, dataset);
}
/**
*
* @param selection
* @param seqstring
* @param product
* - array of DbRefEntry objects from which exon map in seqstring is
* derived
* @param viscontigs
* @param gapCharacter
* @param annotations
* @param aWidth
* @param dataset
* @return
*/
public static AlignmentI CdnaTranslate(SequenceI[] selection,
String[] seqstring, DBRefEntry[] product, int viscontigs[],
char gapCharacter, AlignmentAnnotation[] annotations, int aWidth,
Alignment dataset)
{
AlignedCodonFrame codons = new AlignedCodonFrame(aWidth); // stores hash of
// subsequent
// positions for
// each codon
// start position
// in alignment
int s, sSize = selection.length;
Vector pepseqs = new Vector();
for (s = 0; s < sSize; s++)
{
SequenceI newseq = translateCodingRegion(selection[s], seqstring[s],
viscontigs, codons, gapCharacter,
(product != null) ? product[s] : null, false); // possibly anonymous
// product
if (newseq != null)
{
pepseqs.addElement(newseq);
SequenceI ds = newseq;
if (dataset != null)
{
while (ds.getDatasetSequence() != null)
{
ds = ds.getDatasetSequence();
}
dataset.addSequence(ds);
}
}
}
if (codons.aaWidth == 0)
return null;
SequenceI[] newseqs = new SequenceI[pepseqs.size()];
pepseqs.copyInto(newseqs);
AlignmentI al = new Alignment(newseqs);
al.padGaps(); // ensure we look aligned.
al.setDataset(dataset);
translateAlignedAnnotations(annotations, al, codons);
al.addCodonFrame(codons);
return al;
}
/**
* fake the collection of DbRefs with associated exon mappings to identify if
* a translation would generate distinct product in the currently selected
* region.
*
* @param selection
* @param viscontigs
* @return
*/
public static boolean canTranslate(SequenceI[] selection,
int viscontigs[])
{
for (int gd = 0; gd < selection.length; gd++)
{
SequenceI dna = selection[gd];
jalview.datamodel.DBRefEntry[] dnarefs = jalview.util.DBRefUtils
.selectRefs(dna.getDBRef(),
jalview.datamodel.DBRefSource.DNACODINGDBS);
if (dnarefs != null)
{
// intersect with pep
// intersect with pep
Vector mappedrefs = new Vector();
DBRefEntry[] refs = dna.getDBRef();
for (int d = 0; d < refs.length; d++)
{
if (refs[d].getMap() != null && refs[d].getMap().getMap() != null
&& refs[d].getMap().getMap().getFromRatio() == 3
&& refs[d].getMap().getMap().getToRatio() == 1)
{
mappedrefs.addElement(refs[d]); // add translated protein maps
}
}
dnarefs = new DBRefEntry[mappedrefs.size()];
mappedrefs.copyInto(dnarefs);
for (int d = 0; d < dnarefs.length; d++)
{
Mapping mp = dnarefs[d].getMap();
if (mp != null)
{
for (int vc = 0; vc < viscontigs.length; vc += 2)
{
int[] mpr = mp.locateMappedRange(viscontigs[vc],
viscontigs[vc + 1]);
if (mpr != null)
{
return true;
}
}
}
}
}
}
return false;
}
/**
* generate a set of translated protein products from annotated sequenceI
*
* @param selection
* @param viscontigs
* @param gapCharacter
* @param dataset
* destination dataset for translated sequences
* @param annotations
* @param aWidth
* @return
*/
public static AlignmentI CdnaTranslate(SequenceI[] selection,
int viscontigs[], char gapCharacter, Alignment dataset)
{
int alwidth = 0;
Vector cdnasqs = new Vector();
Vector cdnasqi = new Vector();
Vector cdnaprod = new Vector();
for (int gd = 0; gd < selection.length; gd++)
{
SequenceI dna = selection[gd];
jalview.datamodel.DBRefEntry[] dnarefs = jalview.util.DBRefUtils
.selectRefs(dna.getDBRef(),
jalview.datamodel.DBRefSource.DNACODINGDBS);
if (dnarefs != null)
{
// intersect with pep
Vector mappedrefs = new Vector();
DBRefEntry[] refs = dna.getDBRef();
for (int d = 0; d < refs.length; d++)
{
if (refs[d].getMap() != null && refs[d].getMap().getMap() != null
&& refs[d].getMap().getMap().getFromRatio() == 3
&& refs[d].getMap().getMap().getToRatio() == 1)
{
mappedrefs.addElement(refs[d]); // add translated protein maps
}
}
dnarefs = new DBRefEntry[mappedrefs.size()];
mappedrefs.copyInto(dnarefs);
for (int d = 0; d < dnarefs.length; d++)
{
Mapping mp = dnarefs[d].getMap();
StringBuffer sqstr = new StringBuffer();
if (mp != null)
{
Mapping intersect = mp.intersectVisContigs(viscontigs);
// generate seqstring for this sequence based on mapping
if (sqstr.length() > alwidth)
alwidth = sqstr.length();
cdnasqs.addElement(sqstr.toString());
cdnasqi.addElement(dna);
cdnaprod.addElement(intersect);
}
}
}
SequenceI[] cdna = new SequenceI[cdnasqs.size()];
DBRefEntry[] prods = new DBRefEntry[cdnaprod.size()];
String[] xons = new String[cdnasqs.size()];
cdnasqs.copyInto(xons);
cdnaprod.copyInto(prods);
cdnasqi.copyInto(cdna);
return CdnaTranslate(cdna, xons, prods, viscontigs, gapCharacter,
null, alwidth, dataset);
}
return null;
}
/**
* translate na alignment annotations onto translated amino acid alignment al
* using codon mapping codons
*
* @param annotations
* @param al
* @param codons
*/
public static void translateAlignedAnnotations(
AlignmentAnnotation[] annotations, AlignmentI al,
AlignedCodonFrame codons)
{
// //////////////////////////////
// Copy annotations across
//
// Can only do this for columns with consecutive codons, or where
// annotation is sequence associated.
int pos, a, aSize;
if (annotations != null)
{
for (int i = 0; i < annotations.length; i++)
{
// Skip any autogenerated annotation
if (annotations[i].autoCalculated)
{
continue;
}
aSize = codons.getaaWidth(); // aa alignment width.
jalview.datamodel.Annotation[] anots = (annotations[i].annotations == null) ? null
: new jalview.datamodel.Annotation[aSize];
if (anots != null)
{
for (a = 0; a < aSize; a++)
{
// process through codon map.
if (codons.codons[a] != null
&& codons.codons[a][0] == (codons.codons[a][2] - 2))
{
anots[a] = getCodonAnnotation(codons.codons[a],
annotations[i].annotations);
}
}
}
jalview.datamodel.AlignmentAnnotation aa = new jalview.datamodel.AlignmentAnnotation(
annotations[i].label, annotations[i].description, anots);
aa.graph = annotations[i].graph;
aa.graphGroup = annotations[i].graphGroup;
aa.graphHeight = annotations[i].graphHeight;
if (annotations[i].getThreshold() != null)
{
aa.setThreshold(new jalview.datamodel.GraphLine(annotations[i]
.getThreshold()));
}
if (annotations[i].hasScore)
{
aa.setScore(annotations[i].getScore());
}
if (annotations[i].sequenceRef != null)
{
SequenceI aaSeq = codons
.getAaForDnaSeq(annotations[i].sequenceRef);
if (aaSeq != null)
{
// aa.compactAnnotationArray(); // throw away alignment annotation
// positioning
aa.setSequenceRef(aaSeq);
aa.createSequenceMapping(aaSeq, aaSeq.getStart(), true); // rebuild
// mapping
aa.adjustForAlignment();
aaSeq.addAlignmentAnnotation(aa);
}
}
al.addAnnotation(aa);
}
}
}
private static Annotation getCodonAnnotation(int[] is,
Annotation[] annotations)
{
// Have a look at all the codon positions for annotation and put the first
// one found into the translated annotation pos.
int contrib = 0;
Annotation annot = null;
for (int p = 0; p < 3; p++)
{
if (annotations[is[p]] != null)
{
if (annot == null)
{
annot = new Annotation(annotations[is[p]]);
contrib = 1;
}
else
{
// merge with last
Annotation cpy = new Annotation(annotations[is[p]]);
if (annot.colour == null)
{
annot.colour = cpy.colour;
}
if (annot.description == null || annot.description.length() == 0)
{
annot.description = cpy.description;
}
if (annot.displayCharacter == null)
{
annot.displayCharacter = cpy.displayCharacter;
}
if (annot.secondaryStructure == 0)
{
annot.secondaryStructure = cpy.secondaryStructure;
}
annot.value += cpy.value;
contrib++;
}
}
}
if (contrib > 1)
{
annot.value /= (float) contrib;
}
return annot;
}
/**
* Translate a na sequence
*
* @param selection
* sequence displayed under viscontigs visible columns
* @param seqstring
* ORF read in some global alignment reference frame
* @param viscontigs
* mapping from global reference frame to visible seqstring ORF read
* @param codons
* Definition of global ORF alignment reference frame
* @param gapCharacter
* @return sequence ready to be added to alignment.
* @deprecated Use {@link #translateCodingRegion(SequenceI,String,int[],AlignedCodonFrame,char,DBRefEntry,boolean)} instead
*/
public static SequenceI translateCodingRegion(SequenceI selection,
String seqstring, int[] viscontigs, AlignedCodonFrame codons,
char gapCharacter, DBRefEntry product)
{
return translateCodingRegion(selection, seqstring, viscontigs, codons,
gapCharacter, product, false);
}
/**
* Translate a na sequence
*
* @param selection
* sequence displayed under viscontigs visible columns
* @param seqstring
* ORF read in some global alignment reference frame
* @param viscontigs
* mapping from global reference frame to visible seqstring ORF read
* @param codons
* Definition of global ORF alignment reference frame
* @param gapCharacter
* @param starForStop when true stop codons will translate as '*', otherwise as 'X'
* @return sequence ready to be added to alignment.
*/
public static SequenceI translateCodingRegion(SequenceI selection,
String seqstring, int[] viscontigs, AlignedCodonFrame codons,
char gapCharacter, DBRefEntry product, final boolean starForStop)
{
java.util.List skip = new ArrayList();
int skipint[] = null;
ShiftList vismapping = new ShiftList(); // map from viscontigs to seqstring
// intervals
int vc, scontigs[] = new int[viscontigs.length];
int npos = 0;
for (vc = 0; vc < viscontigs.length; vc += 2)
{
if (vc == 0)
{
vismapping.addShift(npos, viscontigs[vc]);
}
else
{
// hidden region
vismapping.addShift(npos, viscontigs[vc] - viscontigs[vc - 1] + 1);
}
scontigs[vc] = viscontigs[vc];
scontigs[vc + 1] = viscontigs[vc + 1];
}
StringBuffer protein = new StringBuffer();
String seq = seqstring.replace('U', 'T');
char codon[] = new char[3];
int cdp[] = new int[3], rf = 0, lastnpos = 0, nend;
int aspos = 0;
int resSize = 0;
for (npos = 0, nend = seq.length(); npos < nend; npos++)
{
if (!jalview.util.Comparison.isGap(seq.charAt(npos)))
{
cdp[rf] = npos; // store position
codon[rf++] = seq.charAt(npos); // store base
}
// filled an RF yet ?
if (rf == 3)
{
String aa = ResidueProperties.codonTranslate(new String(codon));
rf = 0;
if (aa == null)
{
aa = String.valueOf(gapCharacter);
if (skipint == null)
{
skipint = new int[]
{ cdp[0], cdp[2] };
}
skipint[1] = cdp[2];
}
else
{
if (skipint != null)
{
// edit scontigs
skipint[0] = vismapping.shift(skipint[0]);
skipint[1] = vismapping.shift(skipint[1]);
for (vc = 0; vc < scontigs.length; )
{
if (scontigs[vc + 1] < skipint[0])
{
// before skipint starts
vc += 2;
continue;
}
if (scontigs[vc]>skipint[1])
{
// finished editing so
break;
}
// Edit the contig list to include the skipped region which did not translate
int[] t;
// from : s1 e1 s2 e2 s3 e3
// to s: s1 e1 s2 k0 k1 e2 s3 e3
// list increases by one unless one boundary (s2==k0 or e2==k1) matches, and decreases by one if skipint intersects whole visible contig
if (scontigs[vc] <= skipint[0])
{
if (skipint[0] == scontigs[vc])
{
// skipint at start of contig
// shift the start of this contig
if (scontigs[vc + 1] > skipint[1])
{
scontigs[vc] = skipint[1];
vc+=2;
}
else
{
if (scontigs[vc+1]==skipint[1])
{
// remove the contig
t = new int[scontigs.length - 2];
if (vc > 0)
{
System.arraycopy(scontigs, 0, t, 0, vc - 1);
}
if (vc + 2 < t.length)
{
System.arraycopy(scontigs, vc + 2, t, vc, t.length
- vc + 2);
}
scontigs=t;
} else {
// truncate contig to before the skipint region
scontigs[vc+1] = skipint[0]-1;
vc+=2;
}
}
}
else
{
// scontig starts before start of skipint
if (scontigs[vc+1]= codons.aaWidth)
// codons.aaWidth = aspos + 1;
break; // check the next position for alignment
case 0:
// codon aligns at aspos position.
findpos = false;
}
}
// codon aligns with all other sequence residues found at aspos
protein.append(aa);
lastnpos = npos;
if (codons.codons[aspos] == null)
{
// mark this column as aligning to this aligned reading frame
codons.codons[aspos] = new int[]
{ cdp[0], cdp[1], cdp[2] };
}
if (aspos >= codons.aaWidth)
{
// update maximum alignment width
// (we can do this without calling checkCodonFrameWidth because it was
// already done above)
codons.setAaWidth(aspos);
}
// ready for next translated reading frame alignment position (if any)
aspos++;
}
}
if (resSize > 0)
{
SequenceI newseq = new Sequence(selection.getName(),
protein.toString());
if (rf != 0)
{
if (jalview.bin.Cache.log!=null) {
jalview.bin.Cache.log.debug("trimming contigs for incomplete terminal codon.");
} else {
System.err.println("trimming contigs for incomplete terminal codon.");
}
// map and trim contigs to ORF region
vc = scontigs.length - 1;
lastnpos = vismapping.shift(lastnpos); // place npos in context of
// whole dna alignment (rather
// than visible contigs)
// incomplete ORF could be broken over one or two visible contig
// intervals.
while (vc >= 0 && scontigs[vc] > lastnpos)
{
if (vc > 0 && scontigs[vc - 1] > lastnpos)
{
vc -= 2;
}
else
{
// correct last interval in list.
scontigs[vc] = lastnpos;
}
}
if (vc > 0 && (vc + 1) < scontigs.length)
{
// truncate map list to just vc elements
int t[] = new int[vc + 1];
System.arraycopy(scontigs, 0, t, 0, vc + 1);
scontigs = t;
}
if (vc <= 0)
scontigs = null;
}
if (scontigs != null)
{
npos = 0;
// map scontigs to actual sequence positions on selection
for (vc = 0; vc < scontigs.length; vc += 2)
{
scontigs[vc] = selection.findPosition(scontigs[vc]); // not from 1!
scontigs[vc + 1] = selection.findPosition(scontigs[vc + 1]); // exclusive
if (scontigs[vc + 1] == selection.getEnd())
break;
}
// trim trailing empty intervals.
if ((vc + 2) < scontigs.length)
{
int t[] = new int[vc + 2];
System.arraycopy(scontigs, 0, t, 0, vc + 2);
scontigs = t;
}
/*
* delete intervals in scontigs which are not translated. 1. map skip
* into sequence position intervals 2. truncate existing ranges and add
* new ranges to exclude untranslated regions. if (skip.size()>0) {
* Vector narange = new Vector(); for (vc=0; vc=skipint[0] &&
* iv[0]<=skipint[1]) { if (iv[0]==skipint[0]) { // delete beginning of
* range } else { // truncate range and create new one if necessary iv =
* (int[]) narange.elementAt(vc+1); if (iv[0]<=skipint[1]) { // truncate
* range iv[0] = skipint[1]; } else { } } } else if (iv[0]