/*
* Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
* Copyright (C) $$Year-Rel$$ The Jalview Authors
*
* This file is part of Jalview.
*
* Jalview is free software: you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation, either version 3
* of the License, or (at your option) any later version.
*
* Jalview is distributed in the hope that it will be useful, but
* WITHOUT ANY WARRANTY; without even the implied warranty
* of MERCHANTABILITY or FITNESS FOR A PARTICULAR
* PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Jalview. If not, see .
* The Jalview Authors are detailed in the 'AUTHORS' file.
*/
package jalview.ext.htsjdk;
import jalview.datamodel.Sequence;
import jalview.datamodel.SequenceI;
import java.io.File;
import java.io.IOException;
import java.math.BigInteger;
import java.nio.file.Path;
import java.security.MessageDigest;
import java.security.NoSuchAlgorithmException;
import java.util.ArrayList;
import java.util.HashSet;
import java.util.List;
import java.util.Set;
import htsjdk.samtools.SAMException;
import htsjdk.samtools.SAMSequenceDictionary;
import htsjdk.samtools.SAMSequenceRecord;
import htsjdk.samtools.reference.FastaSequenceIndexCreator;
import htsjdk.samtools.reference.ReferenceSequence;
import htsjdk.samtools.reference.ReferenceSequenceFile;
import htsjdk.samtools.reference.ReferenceSequenceFileFactory;
import htsjdk.samtools.util.StringUtil;
/**
* a source of sequence data accessed via the HTSJDK
*
* @author jprocter
*
*/
public class HtsContigDb
{
private String name;
private File dbLocation;
private htsjdk.samtools.reference.ReferenceSequenceFile refFile = null;
public static void createFastaSequenceIndex(Path path, boolean overwrite)
throws IOException
{
try
{
FastaSequenceIndexCreator.create(path, overwrite);
} catch (SAMException e)
{
throw new IOException(e.getMessage());
}
}
public HtsContigDb(String name, File descriptor)
{
if (descriptor.isFile())
{
this.name = name;
dbLocation = descriptor;
}
initSource();
}
public void close()
{
if (refFile != null)
{
try
{
refFile.close();
} catch (IOException e)
{
// ignore
}
}
}
private void initSource()
{
if (refFile != null)
{
return;
}
refFile = ReferenceSequenceFileFactory
.getReferenceSequenceFile(dbLocation, true);
if (refFile == null || refFile.getSequenceDictionary() == null)
{
// refFile = initSequenceDictionaryFor(dbLocation);
}
}
SAMSequenceDictionary rrefDict = null;
private ReferenceSequenceFile initSequenceDictionaryFor(File dbLocation2)
throws Exception
{
rrefDict = getDictionary(dbLocation2, true);
if (rrefDict != null)
{
ReferenceSequenceFile rrefFile = ReferenceSequenceFileFactory
.getReferenceSequenceFile(dbLocation2, true);
return rrefFile;
}
return null;
}
/**
* code below hacked out from picard ----
*
* picard/src/java/picard/sam/CreateSequenceDictionary.java
* https://github.com/
* broadinstitute/picard/commit/270580d3e28123496576f0b91b3433179bb5d876
*/
/*
* The MIT License
*
* Copyright (c) 2009 The Broad Institute
*
* Permission is hereby granted, free of charge, to any person obtaining a
* copy of this software and associated documentation files (the "Software"),
* to deal in the Software without restriction, including without limitation
* the rights to use, copy, modify, merge, publish, distribute, sublicense,
* and/or sell copies of the Software, and to permit persons to whom the
* Software is furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
* FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
* DEALINGS IN THE SOFTWARE.
*/
/**
*
* @param f
* @param truncate
* @return
* @throws Exception
*/
SAMSequenceDictionary getDictionary(File f, boolean truncate)
throws Exception
{
if (md5 == null)
{
initCreateSequenceDictionary();
}
final ReferenceSequenceFile refSeqFile = ReferenceSequenceFileFactory
.getReferenceSequenceFile(f, truncate);
ReferenceSequence refSeq;
List ret = new ArrayList<>();
Set sequenceNames = new HashSet<>();
for (int numSequences = 0; (refSeq = refSeqFile
.nextSequence()) != null; ++numSequences)
{
if (sequenceNames.contains(refSeq.getName()))
{
throw new Exception(
"Sequence name appears more than once in reference: "
+ refSeq.getName());
}
sequenceNames.add(refSeq.getName());
ret.add(makeSequenceRecord(refSeq));
}
return new SAMSequenceDictionary(ret);
}
public boolean isValid()
{
return dbLocation != null && refFile != null;
}
/**
* Create one SAMSequenceRecord from a single fasta sequence
*/
private SAMSequenceRecord makeSequenceRecord(
final ReferenceSequence refSeq)
{
final SAMSequenceRecord ret = new SAMSequenceRecord(refSeq.getName(),
refSeq.length());
// Compute MD5 of upcased bases
final byte[] bases = refSeq.getBases();
for (int i = 0; i < bases.length; ++i)
{
bases[i] = StringUtil.toUpperCase(bases[i]);
}
ret.setAttribute(SAMSequenceRecord.MD5_TAG, md5Hash(bases));
// if (GENOME_ASSEMBLY != null) {
// ret.setAttribute(SAMSequenceRecord.ASSEMBLY_TAG, GENOME_ASSEMBLY);
// }
// ret.setAttribute(SAMSequenceRecord.URI_TAG, URI);
// if (SPECIES != null) {
// ret.setAttribute(SAMSequenceRecord.SPECIES_TAG, SPECIES);
// }
return ret;
}
private MessageDigest md5;
public void initCreateSequenceDictionary() throws Exception
{
try
{
md5 = MessageDigest.getInstance("MD5");
} catch (NoSuchAlgorithmException e)
{
throw new Exception("MD5 algorithm not found", e);
}
}
private String md5Hash(final byte[] bytes)
{
md5.reset();
md5.update(bytes);
String s = new BigInteger(1, md5.digest()).toString(16);
if (s.length() != 32)
{
final String zeros = "00000000000000000000000000000000";
s = zeros.substring(0, 32 - s.length()) + s;
}
return s;
}
// ///// end of hts bits.
/**
* Reads the contig with the given id and returns as a Jalview SequenceI
* object. Note the database must be indexed for this operation to succeed.
*
* @param id
* @return
*/
public SequenceI getSequenceProxy(String id)
{
if (!isValid() || !refFile.isIndexed())
{
jalview.bin.Console.errPrintln(
"Cannot read contig as file is invalid or not indexed");
return null;
}
ReferenceSequence sseq = refFile.getSequence(id);
return new Sequence(sseq.getName(), new String(sseq.getBases()));
}
public boolean isIndexed()
{
return refFile != null && refFile.isIndexed();
}
}