/* * Jalview - A Sequence Alignment Editor and Viewer * Copyright (C) 2005 AM Waterhouse, J Procter, G Barton, M Clamp, S Searle * * This program is free software; you can redistribute it and/or * modify it under the terms of the GNU General Public License * as published by the Free Software Foundation; either version 2 * of the License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public License * along with this program; if not, write to the Free Software * Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA */ package jalview.gui; import jalview.analysis.*; import jalview.datamodel.*; import jalview.jbgui.*; import java.awt.*; import java.awt.event.*; import java.util.*; import javax.swing.*; import java.awt.print.*; /** * DOCUMENT ME! * * @author $author$ * @version $Revision$ */ public class PCAPanel extends GPCAPanel implements Runnable { PCA pca; int top; RotatableCanvas rc; AlignViewport av; SequenceI [] seqs; /** * Creates a new PCAPanel object. * * @param av DOCUMENT ME! * @param s DOCUMENT ME! */ public PCAPanel(AlignViewport av) { this.av = av; boolean sameLength = true; if ((av.getSelectionGroup() != null) && (av.getSelectionGroup().getSize(false) > 3)) { seqs = new Sequence[av.getSelectionGroup().getSize(false)]; int length = av.getSelectionGroup().getSequenceAt(0).getLength(); for (int i = 0; i < av.getSelectionGroup().getSize(false); i++) { seqs[i] = av.getSelectionGroup().getSequenceAt(i); if(seqs[i].getLength()!=length) { sameLength = false; break; } } } else { seqs = new Sequence[av.getAlignment().getHeight()]; int length = av.alignment.getSequenceAt(0).getLength(); for (int i = 0; i < av.getAlignment().getHeight(); i++) { seqs[i] = av.getAlignment().getSequenceAt(i); if(seqs[i].getLength()!=length) { sameLength = false; break; } } } if (!sameLength) { JOptionPane.showMessageDialog(Desktop.desktop, "The sequences must be aligned before calculating PCA.\n" + "Try using the Pad function in the edit menu,\n" + "or one of the multiple sequence alignment web services.", "Sequences not aligned", JOptionPane.WARNING_MESSAGE); return; } Desktop.addInternalFrame(this, "Principal component analysis", 400, 400); rc = new RotatableCanvas(av); this.getContentPane().add(rc, BorderLayout.CENTER); Thread worker = new Thread(this); worker.start(); } public void bgcolour_actionPerformed(ActionEvent e) { Color col = JColorChooser.showDialog(this, "Select Background Colour", rc.bgColour); if(col!=null) rc.bgColour = col; rc.repaint(); } /** * DOCUMENT ME! */ public void run() { try{ pca = new PCA(seqs); pca.run(); // Now find the component coordinates int ii = 0; while ( (ii < seqs.length) && (seqs[ii] != null)) { ii++; } double[][] comps = new double[ii][ii]; for (int i = 0; i < ii; i++) { if (pca.getEigenvalue(i) > 1e-4) { comps[i] = pca.component(i); } } ////////////////// xCombobox.setSelectedIndex(0); yCombobox.setSelectedIndex(1); zCombobox.setSelectedIndex(2); top = pca.getM().rows - 1; Vector points = new Vector(); float[][] scores = pca.getComponents(top - 1, top - 2, top - 3, 100); for (int i = 0; i < pca.getM().rows; i++) { SequencePoint sp = new SequencePoint(seqs[i], scores[i]); points.addElement(sp); } rc.setPoints(points, pca.getM().rows); rc.repaint(); seqs = null; } catch(OutOfMemoryError er) { JOptionPane.showInternalMessageDialog(Desktop.desktop, "Out of memory calculating PCA!!" + "\nSee help files for increasing Java Virtual Machine memory." , "Out of memory", JOptionPane.WARNING_MESSAGE); System.out.println("PCAPanel: "+er); System.gc(); } } /** * DOCUMENT ME! */ void doDimensionChange() { if (top == 0) { return; } int dim1 = top - xCombobox.getSelectedIndex(); int dim2 = top - yCombobox.getSelectedIndex(); int dim3 = top - zCombobox.getSelectedIndex(); float[][] scores = pca.getComponents(dim1, dim2, dim3, 100); for (int i = 0; i < pca.getM().rows; i++) { ((SequencePoint) rc.points.elementAt(i)).coord = scores[i]; } rc.img = null; rc.rotmat.setIdentity(); rc.initAxes(); rc.paint(rc.getGraphics()); } /** * DOCUMENT ME! * * @param e DOCUMENT ME! */ protected void xCombobox_actionPerformed(ActionEvent e) { doDimensionChange(); } /** * DOCUMENT ME! * * @param e DOCUMENT ME! */ protected void yCombobox_actionPerformed(ActionEvent e) { doDimensionChange(); } /** * DOCUMENT ME! * * @param e DOCUMENT ME! */ protected void zCombobox_actionPerformed(ActionEvent e) { doDimensionChange(); } public void outputValues_actionPerformed(ActionEvent e) { CutAndPasteTransfer cap = new CutAndPasteTransfer(); Desktop.addInternalFrame(cap, "PCA details", 500, 500); cap.setText(pca.getDetails()); } public void showLabels_actionPerformed(ActionEvent e) { rc.showLabels(showLabels.getState()); } public void print_actionPerformed(ActionEvent e) { PCAPrinter printer = new PCAPrinter(); printer.start(); } class PCAPrinter extends Thread implements Printable { public void run() { PrinterJob printJob = PrinterJob.getPrinterJob(); PageFormat pf = printJob.pageDialog(printJob.defaultPage()); printJob.setPrintable(this, pf); if (printJob.printDialog()) { try { printJob.print(); } catch (Exception PrintException) { PrintException.printStackTrace(); } } } public int print(Graphics pg, PageFormat pf, int pi) throws PrinterException { pg.translate( (int) pf.getImageableX(), (int) pf.getImageableY()); rc.drawBackground(pg, rc.bgColour); rc.drawScene(pg); if (rc.drawAxes == true) { rc.drawAxes(pg); } if (pi == 0) return Printable.PAGE_EXISTS; else return Printable.NO_SUCH_PAGE; } } /** * DOCUMENT ME! * * @param e DOCUMENT ME! */ public void eps_actionPerformed(ActionEvent e) { makePCAImage(jalview.util.ImageMaker.EPS); } /** * DOCUMENT ME! * * @param e DOCUMENT ME! */ public void png_actionPerformed(ActionEvent e) { makePCAImage(jalview.util.ImageMaker.PNG); } void makePCAImage(int type) { int width = rc.getWidth(); int height = rc.getHeight(); jalview.util.ImageMaker im; if(type == jalview.util.ImageMaker.PNG) im = new jalview.util.ImageMaker(this, jalview.util.ImageMaker.PNG, "Make PNG image from PCA", width, height, null, null); else im = new jalview.util.ImageMaker(this, jalview.util.ImageMaker.EPS, "Make EPS file from PCA", width, height, null, this.getTitle()); if(im.getGraphics()!=null) { rc.drawBackground(im.getGraphics(), Color.black); rc.drawScene(im.getGraphics()); if (rc.drawAxes == true) { rc.drawAxes(im.getGraphics()); } im.writeImage(); } } }