/* * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$) * Copyright (C) $$Year-Rel$$ The Jalview Authors * * This file is part of Jalview. * * Jalview is free software: you can redistribute it and/or * modify it under the terms of the GNU General Public License * as published by the Free Software Foundation, either version 3 * of the License, or (at your option) any later version. * * Jalview is distributed in the hope that it will be useful, but * WITHOUT ANY WARRANTY; without even the implied warranty * of MERCHANTABILITY or FITNESS FOR A PARTICULAR * PURPOSE. See the GNU General Public License for more details. * * You should have received a copy of the GNU General Public License * along with Jalview. If not, see . * The Jalview Authors are detailed in the 'AUTHORS' file. */ package jalview.gui; import jalview.analysis.AlignSeq; import jalview.datamodel.Alignment; import jalview.datamodel.AlignmentView; import jalview.datamodel.SequenceGroup; import jalview.datamodel.SequenceI; import jalview.jbgui.GPairwiseAlignPanel; import jalview.util.MessageManager; import jalview.viewmodel.AlignmentViewport; import java.awt.event.ActionEvent; import java.util.Vector; /** * DOCUMENT ME! * * @author $author$ * @version $Revision$ */ public class PairwiseAlignPanel extends GPairwiseAlignPanel { private static final String DASHES = "---------------------\n"; private float[][] scores; private float[][] alignmentScores; // scores used by PaSiMap private int GAP_OPEN_COST; private int GAP_EXTEND_COST; AlignmentViewport av; Vector sequences; private String alignmentOutput; private boolean suppressTextbox; private boolean discardAlignments; /** * Creates a new PairwiseAlignPanel object. * * @param viewport * DOCUMENT ME! * @param endGaps ~ toggle gaps and the beginning and end of sequences */ public PairwiseAlignPanel(AlignmentViewport viewport) { this(viewport, false, 120, 20); // default penalties used in AlignSeq } public PairwiseAlignPanel(AlignmentViewport viewport, boolean endGaps, int gapOpenCost, int gapExtendCost) { super(); this.av = viewport; StringBuilder sb = new StringBuilder(1024); sequences = new Vector(); SequenceGroup selectionGroup = viewport.getSelectionGroup(); boolean isSelection = selectionGroup != null && selectionGroup.getSize() > 0; AlignmentView view = viewport.getAlignmentView(isSelection); // String[] seqStrings = viewport.getViewAsString(true); String[] seqStrings = view .getSequenceStrings(viewport.getGapCharacter()); SequenceI[] seqs; if (isSelection) { seqs = (SequenceI[]) view .getAlignmentAndHiddenColumns(viewport.getGapCharacter())[0]; } else { seqs = av.getAlignment().getSequencesArray(); } String type = (viewport.getAlignment().isNucleotide()) ? AlignSeq.DNA : AlignSeq.PEP; float[][] scores = new float[seqs.length][seqs.length]; float[][] alignmentScores = new float[seqs.length][seqs.length]; double totscore = 0D; int count = seqs.length; suppressTextbox = count<10; discardAlignments = count<15; boolean first = true; for (int i = 1; i < count; i++) { // fill diagonal alignmentScores with Float.NaN alignmentScores[i - 1][i - 1] = Float.NaN; for (int j = 0; j < i; j++) { AlignSeq as = new AlignSeq(seqs[i], seqStrings[i], seqs[j], seqStrings[j], type, gapOpenCost, gapExtendCost); if (as.s1str.length() == 0 || as.s2str.length() == 0) { continue; } as.calcScoreMatrix(); if (endGaps) { as.traceAlignmentWithEndGaps(); } else { as.traceAlignment(); } as.scoreAlignment(); if (!first) { System.out.println(DASHES); textarea.append(DASHES); sb.append(DASHES); } first = false; if (discardAlignments) { as.printAlignment(System.out); } scores[i][j] = as.getMaxScore() / as.getASeq1().length; alignmentScores[i][j] = as.getAlignmentScore(); totscore = totscore + scores[i][j]; if (suppressTextbox) { textarea.append(as.getOutput()); sb.append(as.getOutput()); } if (discardAlignments) { sequences.add(as.getAlignedSeq1()); sequences.add(as.getAlignedSeq2()); } } } alignmentScores[count - 1][count - 1] = Float.NaN; this.scores = scores; this.alignmentScores = alignmentScores; if (count > 2) { printScoreMatrix(seqs, scores, totscore); } alignmentOutput = sb.toString(); } public float[][] getScores() { return this.scores; } public float[][] getAlignmentScores() { return this.alignmentScores; } public String getAlignmentOutput() { return this.alignmentOutput; } /** * Prints a matrix of seqi-seqj pairwise alignment scores to sysout * * @param seqs * @param scores * @param totscore */ protected void printScoreMatrix(SequenceI[] seqs, float[][] scores, double totscore) { System.out .println("Pairwise alignment scaled similarity score matrix\n"); for (int i = 0; i < seqs.length; i++) { System.out.println( String.format("%3d %s", i + 1, seqs[i].getDisplayId(true))); } /* * table heading columns for sequences 1, 2, 3... */ System.out.print("\n "); for (int i = 0; i < seqs.length; i++) { System.out.print(String.format("%7d", i + 1)); } System.out.println(); for (int i = 0; i < seqs.length; i++) { System.out.print(String.format("%3d", i + 1)); for (int j = 0; j < i; j++) { /* * as a fraction of tot score, outputs are 0 <= score <= 1 */ System.out.print(String.format("%7.3f", scores[i][j] / totscore)); } System.out.println(); } System.out.println("\n"); } /** * DOCUMENT ME! * * @param e * DOCUMENT ME! */ @Override protected void viewInEditorButton_actionPerformed(ActionEvent e) { SequenceI[] seq = new SequenceI[sequences.size()]; for (int i = 0; i < sequences.size(); i++) { seq[i] = sequences.elementAt(i); } AlignFrame af = new AlignFrame(new Alignment(seq), AlignFrame.DEFAULT_WIDTH, AlignFrame.DEFAULT_HEIGHT); Desktop.addInternalFrame(af, MessageManager.getString("label.pairwise_aligned_sequences"), AlignFrame.DEFAULT_WIDTH, AlignFrame.DEFAULT_HEIGHT); } }