/*
* Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
* Copyright (C) $$Year-Rel$$ The Jalview Authors
*
* This file is part of Jalview.
*
* Jalview is free software: you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation, either version 3
* of the License, or (at your option) any later version.
*
* Jalview is distributed in the hope that it will be useful, but
* WITHOUT ANY WARRANTY; without even the implied warranty
* of MERCHANTABILITY or FITNESS FOR A PARTICULAR
* PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Jalview. If not, see .
* The Jalview Authors are detailed in the 'AUTHORS' file.
*/
package jalview.io;
import jalview.analysis.SequenceIdMatcher;
import jalview.datamodel.AlignedCodonFrame;
import jalview.datamodel.AlignmentI;
import jalview.datamodel.SequenceDummy;
import jalview.datamodel.SequenceFeature;
import jalview.datamodel.SequenceI;
import jalview.schemes.AnnotationColourGradient;
import jalview.schemes.GraduatedColor;
import jalview.schemes.UserColourScheme;
import jalview.util.Format;
import jalview.util.MapList;
import java.io.IOException;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.HashMap;
import java.util.Hashtable;
import java.util.Iterator;
import java.util.List;
import java.util.Map;
import java.util.StringTokenizer;
import java.util.Vector;
/**
* Parse and create Jalview Features files Detects GFF format features files and
* parses. Does not implement standard print() - call specific printFeatures or
* printGFF. Uses AlignmentI.findSequence(String id) to find the sequence object
* for the features annotation - this normally works on an exact match.
*
* @author AMW
* @version $Revision$
*/
public class FeaturesFile extends AlignFile
{
/**
* work around for GFF interpretation bug where source string becomes
* description rather than a group
*/
private boolean doGffSource = true;
private int gffversion;
/**
* Creates a new FeaturesFile object.
*/
public FeaturesFile()
{
}
/**
* @param inFile
* @param type
* @throws IOException
*/
public FeaturesFile(String inFile, String type) throws IOException
{
super(inFile, type);
}
/**
* @param source
* @throws IOException
*/
public FeaturesFile(FileParse source) throws IOException
{
super(source);
}
/**
* @param parseImmediately
* @param source
* @throws IOException
*/
public FeaturesFile(boolean parseImmediately, FileParse source)
throws IOException
{
super(parseImmediately, source);
}
/**
* @param parseImmediately
* @param inFile
* @param type
* @throws IOException
*/
public FeaturesFile(boolean parseImmediately, String inFile, String type)
throws IOException
{
super(parseImmediately, inFile, type);
}
/**
* Parse GFF or sequence features file using case-independent matching,
* discarding URLs
*
* @param align
* - alignment/dataset containing sequences that are to be annotated
* @param colours
* - hashtable to store feature colour definitions
* @param removeHTML
* - process html strings into plain text
* @return true if features were added
*/
public boolean parse(AlignmentI align, Hashtable colours,
boolean removeHTML)
{
return parse(align, colours, null, removeHTML, false);
}
/**
* Parse GFF or sequence features file optionally using case-independent
* matching, discarding URLs
*
* @param align
* - alignment/dataset containing sequences that are to be annotated
* @param colours
* - hashtable to store feature colour definitions
* @param removeHTML
* - process html strings into plain text
* @param relaxedIdmatching
* - when true, ID matches to compound sequence IDs are allowed
* @return true if features were added
*/
public boolean parse(AlignmentI align, Map colours, boolean removeHTML,
boolean relaxedIdMatching)
{
return parse(align, colours, null, removeHTML, relaxedIdMatching);
}
/**
* Parse GFF or sequence features file optionally using case-independent
* matching
*
* @param align
* - alignment/dataset containing sequences that are to be annotated
* @param colours
* - hashtable to store feature colour definitions
* @param featureLink
* - hashtable to store associated URLs
* @param removeHTML
* - process html strings into plain text
* @return true if features were added
*/
public boolean parse(AlignmentI align, Map colours, Map featureLink,
boolean removeHTML)
{
return parse(align, colours, featureLink, removeHTML, false);
}
@Override
public void addAnnotations(AlignmentI al)
{
// TODO Auto-generated method stub
super.addAnnotations(al);
}
@Override
public void addProperties(AlignmentI al)
{
// TODO Auto-generated method stub
super.addProperties(al);
}
@Override
public void addSeqGroups(AlignmentI al)
{
// TODO Auto-generated method stub
super.addSeqGroups(al);
}
/**
* Parse GFF or sequence features file
*
* @param align
* - alignment/dataset containing sequences that are to be annotated
* @param colours
* - hashtable to store feature colour definitions
* @param featureLink
* - hashtable to store associated URLs
* @param removeHTML
* - process html strings into plain text
* @param relaxedIdmatching
* - when true, ID matches to compound sequence IDs are allowed
* @return true if features were added
*/
public boolean parse(AlignmentI align, Map colours, Map featureLink,
boolean removeHTML, boolean relaxedIdmatching)
{
String line = null;
try
{
SequenceI seq = null;
/**
* keep track of any sequences we try to create from the data if it is a GFF3 file
*/
ArrayList newseqs = new ArrayList();
String type, desc, token = null;
int index, start, end;
float score;
StringTokenizer st;
SequenceFeature sf;
String featureGroup = null, groupLink = null;
Map typeLink = new Hashtable();
/**
* when true, assume GFF style features rather than Jalview style.
*/
boolean GFFFile = true;
Map gffProps = new HashMap();
while ((line = nextLine()) != null)
{
// skip comments/process pragmas
if (line.startsWith("#"))
{
if (line.startsWith("##"))
{
// possibly GFF2/3 version and metadata header
processGffPragma(line, gffProps, align, newseqs);
line = "";
}
continue;
}
st = new StringTokenizer(line, "\t");
if (st.countTokens() == 1)
{
if (line.trim().equalsIgnoreCase("GFF"))
{
// Start parsing file as if it might be GFF again.
GFFFile = true;
continue;
}
}
if (st.countTokens() > 1 && st.countTokens() < 4)
{
GFFFile = false;
type = st.nextToken();
if (type.equalsIgnoreCase("startgroup"))
{
featureGroup = st.nextToken();
if (st.hasMoreElements())
{
groupLink = st.nextToken();
featureLink.put(featureGroup, groupLink);
}
}
else if (type.equalsIgnoreCase("endgroup"))
{
// We should check whether this is the current group,
// but at present theres no way of showing more than 1 group
st.nextToken();
featureGroup = null;
groupLink = null;
}
else
{
Object colour = null;
String colscheme = st.nextToken();
if (colscheme.indexOf("|") > -1
|| colscheme.trim().equalsIgnoreCase("label"))
{
// Parse '|' separated graduated colourscheme fields:
// [label|][mincolour|maxcolour|[absolute|]minvalue|maxvalue|thresholdtype|thresholdvalue]
// can either provide 'label' only, first is optional, next two
// colors are required (but may be
// left blank), next is optional, nxt two min/max are required.
// first is either 'label'
// first/second and third are both hexadecimal or word equivalent
// colour.
// next two are values parsed as floats.
// fifth is either 'above','below', or 'none'.
// sixth is a float value and only required when fifth is either
// 'above' or 'below'.
StringTokenizer gcol = new StringTokenizer(colscheme, "|",
true);
// set defaults
int threshtype = AnnotationColourGradient.NO_THRESHOLD;
float min = Float.MIN_VALUE, max = Float.MAX_VALUE, threshval = Float.NaN;
boolean labelCol = false;
// Parse spec line
String mincol = gcol.nextToken();
if (mincol == "|")
{
System.err
.println("Expected either 'label' or a colour specification in the line: "
+ line);
continue;
}
String maxcol = null;
if (mincol.toLowerCase().indexOf("label") == 0)
{
labelCol = true;
mincol = (gcol.hasMoreTokens() ? gcol.nextToken() : null); // skip
// '|'
mincol = (gcol.hasMoreTokens() ? gcol.nextToken() : null);
}
String abso = null, minval, maxval;
if (mincol != null)
{
// at least four more tokens
if (mincol.equals("|"))
{
mincol = "";
}
else
{
gcol.nextToken(); // skip next '|'
}
// continue parsing rest of line
maxcol = gcol.nextToken();
if (maxcol.equals("|"))
{
maxcol = "";
}
else
{
gcol.nextToken(); // skip next '|'
}
abso = gcol.nextToken();
gcol.nextToken(); // skip next '|'
if (abso.toLowerCase().indexOf("abso") != 0)
{
minval = abso;
abso = null;
}
else
{
minval = gcol.nextToken();
gcol.nextToken(); // skip next '|'
}
maxval = gcol.nextToken();
if (gcol.hasMoreTokens())
{
gcol.nextToken(); // skip next '|'
}
try
{
if (minval.length() > 0)
{
min = new Float(minval).floatValue();
}
} catch (Exception e)
{
System.err
.println("Couldn't parse the minimum value for graduated colour for type ("
+ colscheme
+ ") - did you misspell 'auto' for the optional automatic colour switch ?");
e.printStackTrace();
}
try
{
if (maxval.length() > 0)
{
max = new Float(maxval).floatValue();
}
} catch (Exception e)
{
System.err
.println("Couldn't parse the maximum value for graduated colour for type ("
+ colscheme + ")");
e.printStackTrace();
}
}
else
{
// add in some dummy min/max colours for the label-only
// colourscheme.
mincol = "FFFFFF";
maxcol = "000000";
}
try
{
colour = new jalview.schemes.GraduatedColor(
new UserColourScheme(mincol).findColour('A'),
new UserColourScheme(maxcol).findColour('A'), min,
max);
} catch (Exception e)
{
System.err
.println("Couldn't parse the graduated colour scheme ("
+ colscheme + ")");
e.printStackTrace();
}
if (colour != null)
{
((jalview.schemes.GraduatedColor) colour)
.setColourByLabel(labelCol);
((jalview.schemes.GraduatedColor) colour)
.setAutoScaled(abso == null);
// add in any additional parameters
String ttype = null, tval = null;
if (gcol.hasMoreTokens())
{
// threshold type and possibly a threshold value
ttype = gcol.nextToken();
if (ttype.toLowerCase().startsWith("below"))
{
((jalview.schemes.GraduatedColor) colour)
.setThreshType(AnnotationColourGradient.BELOW_THRESHOLD);
}
else if (ttype.toLowerCase().startsWith("above"))
{
((jalview.schemes.GraduatedColor) colour)
.setThreshType(AnnotationColourGradient.ABOVE_THRESHOLD);
}
else
{
((jalview.schemes.GraduatedColor) colour)
.setThreshType(AnnotationColourGradient.NO_THRESHOLD);
if (!ttype.toLowerCase().startsWith("no"))
{
System.err
.println("Ignoring unrecognised threshold type : "
+ ttype);
}
}
}
if (((GraduatedColor) colour).getThreshType() != AnnotationColourGradient.NO_THRESHOLD)
{
try
{
gcol.nextToken();
tval = gcol.nextToken();
((jalview.schemes.GraduatedColor) colour)
.setThresh(new Float(tval).floatValue());
} catch (Exception e)
{
System.err
.println("Couldn't parse threshold value as a float: ("
+ tval + ")");
e.printStackTrace();
}
}
// parse the thresh-is-min token ?
if (gcol.hasMoreTokens())
{
System.err
.println("Ignoring additional tokens in parameters in graduated colour specification\n");
while (gcol.hasMoreTokens())
{
System.err.println("|" + gcol.nextToken());
}
System.err.println("\n");
}
}
}
else
{
UserColourScheme ucs = new UserColourScheme(colscheme);
colour = ucs.findColour('A');
}
if (colour != null)
{
colours.put(type, colour);
}
if (st.hasMoreElements())
{
String link = st.nextToken();
typeLink.put(type, link);
if (featureLink == null)
{
featureLink = new Hashtable();
}
featureLink.put(type, link);
}
}
continue;
}
String seqId = "";
while (st.hasMoreElements())
{
if (GFFFile)
{
// Still possible this is an old Jalview file,
// which does not have type colours at the beginning
seqId = token = st.nextToken();
seq = findName(align, seqId, relaxedIdmatching, newseqs);
if (seq != null)
{
desc = st.nextToken();
String group = null;
if (doGffSource && desc.indexOf(' ') == -1)
{
// could also be a source term rather than description line
group = new String(desc);
}
type = st.nextToken();
try
{
String stt = st.nextToken();
if (stt.length() == 0 || stt.equals("-"))
{
start = 0;
}
else
{
start = Integer.parseInt(stt);
}
} catch (NumberFormatException ex)
{
start = 0;
}
try
{
String stt = st.nextToken();
if (stt.length() == 0 || stt.equals("-"))
{
end = 0;
}
else
{
end = Integer.parseInt(stt);
}
} catch (NumberFormatException ex)
{
end = 0;
}
// TODO: decide if non positional feature assertion for input data
// where end==0 is generally valid
if (end == 0)
{
// treat as non-positional feature, regardless.
start = 0;
}
try
{
score = new Float(st.nextToken()).floatValue();
} catch (NumberFormatException ex)
{
score = 0;
}
sf = new SequenceFeature(type, desc, start, end, score, group);
try
{
sf.setValue("STRAND", st.nextToken());
sf.setValue("FRAME", st.nextToken());
} catch (Exception ex)
{
}
if (st.hasMoreTokens())
{
StringBuffer attributes = new StringBuffer();
boolean sep = false;
while (st.hasMoreTokens())
{
attributes.append((sep ? "\t" : "") + st.nextElement());
sep = true;
}
// TODO validate and split GFF2 attributes field ? parse out
// ([A-Za-z][A-Za-z0-9_]*) ; and add as
// sf.setValue(attrib, val);
sf.setValue("ATTRIBUTES", attributes.toString());
}
if (processOrAddSeqFeature(align, newseqs, seq, sf, GFFFile,
relaxedIdmatching))
{
// check whether we should add the sequence feature to any other
// sequences in the alignment with the same or similar
while ((seq = align.findName(seq, seqId, true)) != null)
{
seq.addSequenceFeature(new SequenceFeature(sf));
}
}
break;
}
}
if (GFFFile && seq == null)
{
desc = token;
}
else
{
desc = st.nextToken();
}
if (!st.hasMoreTokens())
{
System.err
.println("DEBUG: Run out of tokens when trying to identify the destination for the feature.. giving up.");
// in all probability, this isn't a file we understand, so bail
// quietly.
return false;
}
token = st.nextToken();
if (!token.equals("ID_NOT_SPECIFIED"))
{
seq = findName(align, seqId = token, relaxedIdmatching, null);
st.nextToken();
}
else
{
seqId = null;
try
{
index = Integer.parseInt(st.nextToken());
seq = align.getSequenceAt(index);
} catch (NumberFormatException ex)
{
seq = null;
}
}
if (seq == null)
{
System.out.println("Sequence not found: " + line);
break;
}
start = Integer.parseInt(st.nextToken());
end = Integer.parseInt(st.nextToken());
type = st.nextToken();
if (!colours.containsKey(type))
{
// Probably the old style groups file
UserColourScheme ucs = new UserColourScheme(type);
colours.put(type, ucs.findColour('A'));
}
sf = new SequenceFeature(type, desc, "", start, end, featureGroup);
if (st.hasMoreTokens())
{
try
{
score = new Float(st.nextToken()).floatValue();
// update colourgradient bounds if allowed to
} catch (NumberFormatException ex)
{
score = 0;
}
sf.setScore(score);
}
if (groupLink != null && removeHTML)
{
sf.addLink(groupLink);
sf.description += "%LINK%";
}
if (typeLink.containsKey(type) && removeHTML)
{
sf.addLink(typeLink.get(type).toString());
sf.description += "%LINK%";
}
parseDescriptionHTML(sf, removeHTML);
seq.addSequenceFeature(sf);
while (seqId != null
&& (seq = align.findName(seq, seqId, false)) != null)
{
seq.addSequenceFeature(new SequenceFeature(sf));
}
// If we got here, its not a GFFFile
GFFFile = false;
}
}
resetMatcher();
} catch (Exception ex)
{
// should report somewhere useful for UI if necessary
warningMessage = ((warningMessage == null) ? "" : warningMessage)
+ "Parsing error at\n" + line;
System.out.println("Error parsing feature file: " + ex + "\n" + line);
ex.printStackTrace(System.err);
resetMatcher();
return false;
}
return true;
}
private enum GffPragmas
{
gff_version, sequence_region, feature_ontology, attribute_ontology, source_ontology, species_build, fasta, hash
};
private static Map GFFPRAGMA;
static
{
GFFPRAGMA = new HashMap();
GFFPRAGMA.put("sequence-region", GffPragmas.sequence_region);
GFFPRAGMA.put("feature-ontology", GffPragmas.feature_ontology);
GFFPRAGMA.put("#", GffPragmas.hash);
GFFPRAGMA.put("fasta", GffPragmas.fasta);
GFFPRAGMA.put("species-build", GffPragmas.species_build);
GFFPRAGMA.put("source-ontology", GffPragmas.source_ontology);
GFFPRAGMA.put("attribute-ontology", GffPragmas.attribute_ontology);
}
private void processGffPragma(String line, Map gffProps,
AlignmentI align, ArrayList newseqs)
throws IOException
{
// line starts with ##
int spacepos = line.indexOf(' ');
String pragma = spacepos == -1 ? line.substring(2).trim() : line
.substring(2, spacepos);
GffPragmas gffpragma = GFFPRAGMA.get(pragma.toLowerCase());
if (gffpragma == null)
{
return;
}
switch (gffpragma)
{
case gff_version:
try
{
gffversion = Integer.parseInt(line.substring(spacepos + 1));
} finally
{
}
break;
case feature_ontology:
// resolve against specific feature ontology
break;
case attribute_ontology:
// resolve against specific attribute ontology
break;
case source_ontology:
// resolve against specific source ontology
break;
case species_build:
// resolve against specific NCBI taxon version
break;
case hash:
// close off any open feature hierarchies
break;
case fasta:
// process the rest of the file as a fasta file and replace any dummy
// sequence IDs
process_as_fasta(align, newseqs);
break;
default:
// we do nothing ?
System.err.println("Ignoring unknown pragma:\n" + line);
}
}
private void process_as_fasta(AlignmentI align, List newseqs)
throws IOException
{
try
{
mark();
} catch (IOException q)
{
}
FastaFile parser = new FastaFile(this);
List includedseqs = parser.getSeqs();
SequenceIdMatcher smatcher = new SequenceIdMatcher(newseqs);
// iterate over includedseqs, and replacing matching ones with newseqs
// sequences. Generic iterator not used here because we modify includedseqs
// as we go
for (int p = 0, pSize = includedseqs.size(); p < pSize; p++)
{
// search for any dummy seqs that this sequence can be used to update
SequenceI dummyseq = smatcher.findIdMatch(includedseqs.get(p));
if (dummyseq != null)
{
// dummyseq was created so it could be annotated and referred to in
// alignments/codon mappings
SequenceI mseq = includedseqs.get(p);
// mseq is the 'template' imported from the FASTA file which we'll use
// to coomplete dummyseq
if (dummyseq instanceof SequenceDummy)
{
// probably have the pattern wrong
// idea is that a flyweight proxy for a sequence ID can be created for
// 1. stable reference creation
// 2. addition of annotation
// 3. future replacement by a real sequence
// current pattern is to create SequenceDummy objects - a convenience
// constructor for a Sequence.
// problem is that when promoted to a real sequence, all references
// need
// to be updated somehow.
((SequenceDummy) dummyseq).become(mseq);
includedseqs.set(p, dummyseq); // template is no longer needed
}
}
}
// finally add sequences to the dataset
for (SequenceI seq : includedseqs)
{
align.addSequence(seq);
}
}
/**
* take a sequence feature and examine its attributes to decide how it should
* be added to a sequence
*
* @param seq
* - the destination sequence constructed or discovered in the
* current context
* @param sf
* - the base feature with ATTRIBUTES property containing any
* additional attributes
* @param gFFFile
* - true if we are processing a GFF annotation file
* @return true if sf was actually added to the sequence, false if it was
* processed in another way
*/
public boolean processOrAddSeqFeature(AlignmentI align, List newseqs, SequenceI seq, SequenceFeature sf,
boolean gFFFile, boolean relaxedIdMatching)
{
String attr = (String) sf.getValue("ATTRIBUTES");
boolean add = true;
if (gFFFile && attr != null)
{
int nattr=8;
for (String attset : attr.split("\t"))
{
if (attset==null || attset.trim().length()==0)
{
continue;
}
nattr++;
Map> set = new HashMap>();
// normally, only expect one column - 9 - in this field
// the attributes (Gff3) or groups (gff2) field
for (String pair : attset.trim().split(";"))
{
pair = pair.trim();
if (pair.length() == 0)
{
continue;
}
// expect either space seperated (gff2) or '=' separated (gff3)
// key/value pairs here
int eqpos = pair.indexOf('='),sppos = pair.indexOf(' ');
String key = null, value = null;
if (sppos > -1 && (eqpos == -1 || sppos < eqpos))
{
key = pair.substring(0, sppos);
value = pair.substring(sppos + 1);
} else {
if (eqpos > -1 && (sppos == -1 || eqpos < sppos))
{
key = pair.substring(0, eqpos);
value = pair.substring(eqpos + 1);
} else
{
key = pair;
}
}
if (key != null)
{
List vals = set.get(key);
if (vals == null)
{
vals = new ArrayList();
set.put(key, vals);
}
if (value != null)
{
vals.add(value.trim());
}
}
}
try
{
add &= processGffKey(set, nattr, seq, sf, align, newseqs,
relaxedIdMatching); // process decides if
// feature is actually
// added
} catch (InvalidGFF3FieldException ivfe)
{
System.err.println(ivfe);
}
}
}
if (add)
{
seq.addSequenceFeature(sf);
}
return add;
}
public class InvalidGFF3FieldException extends Exception
{
String field, value;
public InvalidGFF3FieldException(String field,
Map> set, String message)
{
super(message + " (Field was " + field + " and value was "
+ set.get(field).toString());
this.field = field;
this.value = set.get(field).toString();
}
}
/**
* take a set of keys for a feature and interpret them
*
* @param set
* @param nattr
* @param seq
* @param sf
* @return
*/
public boolean processGffKey(Map> set, int nattr,
SequenceI seq, SequenceFeature sf, AlignmentI align,
List newseqs, boolean relaxedIdMatching)
throws InvalidGFF3FieldException
{
String attr;
// decide how to interpret according to type
if (sf.getType().equals("similarity"))
{
int strand = sf.getStrand();
// exonerate cdna/protein map
// look for fields
List querySeq = findNames(align, newseqs,
relaxedIdMatching, set.get(attr="Query"));
if (querySeq==null || querySeq.size()!=1)
{
throw new InvalidGFF3FieldException( attr, set,
"Expecting exactly one sequence in Query field (got "
+ set.get(attr) + ")");
}
if (set.containsKey(attr="Align"))
{
// process the align maps and create cdna/protein maps
// ideally, the query sequences are in the alignment, but maybe not...
AlignedCodonFrame alco = new AlignedCodonFrame();
MapList codonmapping = constructCodonMappingFromAlign(set, attr,
strand);
// add codon mapping, and hope!
alco.addMap(seq, querySeq.get(0), codonmapping);
align.addCodonFrame(alco);
// everything that's needed to be done is done
// no features to create here !
return false;
}
}
return true;
}
private MapList constructCodonMappingFromAlign(
Map> set,
String attr, int strand) throws InvalidGFF3FieldException
{
if (strand == 0)
{
throw new InvalidGFF3FieldException(attr, set,
"Invalid strand for a codon mapping (cannot be 0)");
}
List fromrange = new ArrayList(), torange = new ArrayList();
int lastppos = 0, lastpframe = 0;
for (String range : set.get(attr))
{
List ints = new ArrayList();
StringTokenizer st = new StringTokenizer(range, " ");
while (st.hasMoreTokens())
{
String num = st.nextToken();
try
{
ints.add(new Integer(num));
} catch (NumberFormatException nfe)
{
throw new InvalidGFF3FieldException(attr, set,
"Invalid number in field " + num);
}
}
// Align positionInRef positionInQuery LengthInRef
// contig_1146 exonerate:protein2genome:local similarity 8534 11269
// 3652 - . alignment_id 0 ;
// Query DDB_G0269124
// Align 11270 143 120
// corresponds to : 120 bases align at pos 143 in protein to 11270 on
// dna in strand direction
// Align 11150 187 282
// corresponds to : 282 bases align at pos 187 in protein to 11150 on
// dna in strand direction
//
// Align 10865 281 888
// Align 9977 578 1068
// Align 8909 935 375
//
if (ints.size() != 3)
{
throw new InvalidGFF3FieldException(attr, set,
"Invalid number of fields for this attribute ("
+ ints.size() + ")");
}
fromrange.add(new Integer(ints.get(0).intValue()));
fromrange.add(new Integer(ints.get(0).intValue() + strand
* ints.get(2).intValue()));
// how are intron/exon boundaries that do not align in codons
// represented
if (ints.get(1).equals(lastppos) && lastpframe > 0)
{
// extend existing to map
lastppos += ints.get(2) / 3;
lastpframe = ints.get(2) % 3;
torange.set(torange.size() - 1, new Integer(lastppos));
}
else
{
// new to map range
torange.add(ints.get(1));
lastppos = ints.get(1) + ints.get(2) / 3;
lastpframe = ints.get(2) % 3;
torange.add(new Integer(lastppos));
}
}
// from and to ranges must end up being a series of start/end intervals
if (fromrange.size() % 2 == 1)
{
throw new InvalidGFF3FieldException(attr, set,
"Couldn't parse the DNA alignment range correctly");
}
if (torange.size() % 2 == 1)
{
throw new InvalidGFF3FieldException(attr, set,
"Couldn't parse the protein alignment range correctly");
}
// finally, build the map
int[] frommap = new int[fromrange.size()], tomap = new int[torange
.size()];
int p = 0;
for (Integer ip : fromrange)
{
frommap[p++] = ip.intValue();
}
p = 0;
for (Integer ip : torange)
{
tomap[p++] = ip.intValue();
}
return new MapList(frommap, tomap, 3, 1);
}
private List findNames(AlignmentI align,
List newseqs, boolean relaxedIdMatching,
List list)
{
List found = new ArrayList();
for (String seqId : list)
{
SequenceI seq = findName(align, seqId, relaxedIdMatching, newseqs);
if (seq != null)
{
found.add(seq);
}
}
return found;
}
private AlignmentI lastmatchedAl = null;
private SequenceIdMatcher matcher = null;
/**
* clear any temporary handles used to speed up ID matching
*/
private void resetMatcher()
{
lastmatchedAl = null;
matcher = null;
}
private SequenceI findName(AlignmentI align, String seqId,
boolean relaxedIdMatching, List newseqs)
{
SequenceI match = null;
if (relaxedIdMatching)
{
if (lastmatchedAl != align)
{
matcher = new SequenceIdMatcher(
(lastmatchedAl = align).getSequencesArray());
if (newseqs != null)
{
matcher.addAll(newseqs);
}
}
match = matcher.findIdMatch(seqId);
}
else
{
match = align.findName(seqId, true);
if (match == null && newseqs != null)
{
for (SequenceI m : newseqs)
{
if (seqId.equals(m.getName()))
{
return m;
}
}
}
}
if (match==null && newseqs!=null)
{
match = new SequenceDummy(seqId);
if (relaxedIdMatching)
{
matcher.addAll(Arrays.asList(new SequenceI[]
{ match }));
}
// add dummy sequence to the newseqs list
newseqs.add(match);
}
return match;
}
public void parseDescriptionHTML(SequenceFeature sf, boolean removeHTML)
{
if (sf.getDescription() == null)
{
return;
}
jalview.util.ParseHtmlBodyAndLinks parsed = new jalview.util.ParseHtmlBodyAndLinks(
sf.getDescription(), removeHTML, newline);
sf.description = (removeHTML) ? parsed.getNonHtmlContent()
: sf.description;
for (String link : parsed.getLinks())
{
sf.addLink(link);
}
}
/**
* generate a features file for seqs includes non-pos features by default.
*
* @param seqs
* source of sequence features
* @param visible
* hash of feature types and colours
* @return features file contents
*/
public String printJalviewFormat(SequenceI[] seqs, Map visible)
{
return printJalviewFormat(seqs, visible, true, true);
}
/**
* generate a features file for seqs with colours from visible (if any)
*
* @param seqs
* source of features
* @param visible
* hash of Colours for each feature type
* @param visOnly
* when true only feature types in 'visible' will be output
* @param nonpos
* indicates if non-positional features should be output (regardless
* of group or type)
* @return features file contents
*/
public String printJalviewFormat(SequenceI[] seqs, Map visible,
boolean visOnly, boolean nonpos)
{
StringBuffer out = new StringBuffer();
SequenceFeature[] next;
boolean featuresGen = false;
if (visOnly && !nonpos && (visible == null || visible.size() < 1))
{
// no point continuing.
return "No Features Visible";
}
if (visible != null && visOnly)
{
// write feature colours only if we're given them and we are generating
// viewed features
// TODO: decide if feature links should also be written here ?
Iterator en = visible.keySet().iterator();
String type, color;
while (en.hasNext())
{
type = en.next().toString();
if (visible.get(type) instanceof GraduatedColor)
{
GraduatedColor gc = (GraduatedColor) visible.get(type);
color = (gc.isColourByLabel() ? "label|" : "")
+ Format.getHexString(gc.getMinColor()) + "|"
+ Format.getHexString(gc.getMaxColor())
+ (gc.isAutoScale() ? "|" : "|abso|") + gc.getMin() + "|"
+ gc.getMax() + "|";
if (gc.getThreshType() != AnnotationColourGradient.NO_THRESHOLD)
{
if (gc.getThreshType() == AnnotationColourGradient.BELOW_THRESHOLD)
{
color += "below";
}
else
{
if (gc.getThreshType() != AnnotationColourGradient.ABOVE_THRESHOLD)
{
System.err.println("WARNING: Unsupported threshold type ("
+ gc.getThreshType() + ") : Assuming 'above'");
}
color += "above";
}
// add the value
color += "|" + gc.getThresh();
}
else
{
color += "none";
}
}
else if (visible.get(type) instanceof java.awt.Color)
{
color = Format.getHexString((java.awt.Color) visible.get(type));
}
else
{
// legacy support for integer objects containing colour triplet values
color = Format.getHexString(new java.awt.Color(Integer
.parseInt(visible.get(type).toString())));
}
out.append(type);
out.append("\t");
out.append(color);
out.append(newline);
}
}
// Work out which groups are both present and visible
Vector groups = new Vector();
int groupIndex = 0;
boolean isnonpos = false;
for (int i = 0; i < seqs.length; i++)
{
next = seqs[i].getSequenceFeatures();
if (next != null)
{
for (int j = 0; j < next.length; j++)
{
isnonpos = next[j].begin == 0 && next[j].end == 0;
if ((!nonpos && isnonpos)
|| (!isnonpos && visOnly && !visible
.containsKey(next[j].type)))
{
continue;
}
if (next[j].featureGroup != null
&& !groups.contains(next[j].featureGroup))
{
groups.addElement(next[j].featureGroup);
}
}
}
}
String group = null;
do
{
if (groups.size() > 0 && groupIndex < groups.size())
{
group = groups.elementAt(groupIndex).toString();
out.append(newline);
out.append("STARTGROUP\t");
out.append(group);
out.append(newline);
}
else
{
group = null;
}
for (int i = 0; i < seqs.length; i++)
{
next = seqs[i].getSequenceFeatures();
if (next != null)
{
for (int j = 0; j < next.length; j++)
{
isnonpos = next[j].begin == 0 && next[j].end == 0;
if ((!nonpos && isnonpos)
|| (!isnonpos && visOnly && !visible
.containsKey(next[j].type)))
{
// skip if feature is nonpos and we ignore them or if we only
// output visible and it isn't non-pos and it's not visible
continue;
}
if (group != null
&& (next[j].featureGroup == null || !next[j].featureGroup
.equals(group)))
{
continue;
}
if (group == null && next[j].featureGroup != null)
{
continue;
}
// we have features to output
featuresGen = true;
if (next[j].description == null
|| next[j].description.equals(""))
{
out.append(next[j].type + "\t");
}
else
{
if (next[j].links != null
&& next[j].getDescription().indexOf("") == -1)
{
out.append("");
}
out.append(next[j].description + " ");
if (next[j].links != null)
{
for (int l = 0; l < next[j].links.size(); l++)
{
String label = next[j].links.elementAt(l).toString();
String href = label.substring(label.indexOf("|") + 1);
label = label.substring(0, label.indexOf("|"));
if (next[j].description.indexOf(href) == -1)
{
out.append("" + label + "");
}
}
if (next[j].getDescription().indexOf("") == -1)
{
out.append("");
}
}
out.append("\t");
}
out.append(seqs[i].getName());
out.append("\t-1\t");
out.append(next[j].begin);
out.append("\t");
out.append(next[j].end);
out.append("\t");
out.append(next[j].type);
if (!Float.isNaN(next[j].score))
{
out.append("\t");
out.append(next[j].score);
}
out.append(newline);
}
}
}
if (group != null)
{
out.append("ENDGROUP\t");
out.append(group);
out.append(newline);
groupIndex++;
}
else
{
break;
}
} while (groupIndex < groups.size() + 1);
if (!featuresGen)
{
return "No Features Visible";
}
return out.toString();
}
/**
* generate a gff file for sequence features includes non-pos features by
* default.
*
* @param seqs
* @param visible
* @return
*/
public String printGFFFormat(SequenceI[] seqs, Map visible)
{
return printGFFFormat(seqs, visible, true, true);
}
public String printGFFFormat(SequenceI[] seqs, Map visible,
boolean visOnly, boolean nonpos)
{
StringBuffer out = new StringBuffer();
SequenceFeature[] next;
String source;
boolean isnonpos;
for (int i = 0; i < seqs.length; i++)
{
if (seqs[i].getSequenceFeatures() != null)
{
next = seqs[i].getSequenceFeatures();
for (int j = 0; j < next.length; j++)
{
isnonpos = next[j].begin == 0 && next[j].end == 0;
if ((!nonpos && isnonpos)
|| (!isnonpos && visOnly && !visible
.containsKey(next[j].type)))
{
continue;
}
source = next[j].featureGroup;
if (source == null)
{
source = next[j].getDescription();
}
out.append(seqs[i].getName());
out.append("\t");
out.append(source);
out.append("\t");
out.append(next[j].type);
out.append("\t");
out.append(next[j].begin);
out.append("\t");
out.append(next[j].end);
out.append("\t");
out.append(next[j].score);
out.append("\t");
if (next[j].getValue("STRAND") != null)
{
out.append(next[j].getValue("STRAND"));
out.append("\t");
}
else
{
out.append(".\t");
}
if (next[j].getValue("FRAME") != null)
{
out.append(next[j].getValue("FRAME"));
}
else
{
out.append(".");
}
// TODO: verify/check GFF - should there be a /t here before attribute
// output ?
if (next[j].getValue("ATTRIBUTES") != null)
{
out.append(next[j].getValue("ATTRIBUTES"));
}
out.append(newline);
}
}
}
return out.toString();
}
/**
* this is only for the benefit of object polymorphism - method does nothing.
*/
public void parse()
{
// IGNORED
}
/**
* this is only for the benefit of object polymorphism - method does nothing.
*
* @return error message
*/
public String print()
{
return "USE printGFFFormat() or printJalviewFormat()";
}
}