/*
* Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
* Copyright (C) $$Year-Rel$$ The Jalview Authors
*
* This file is part of Jalview.
*
* Jalview is free software: you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation, either version 3
* of the License, or (at your option) any later version.
*
* Jalview is distributed in the hope that it will be useful, but
* WITHOUT ANY WARRANTY; without even the implied warranty
* of MERCHANTABILITY or FITNESS FOR A PARTICULAR
* PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Jalview. If not, see .
* The Jalview Authors are detailed in the 'AUTHORS' file.
*/
package jalview.util;
import jalview.datamodel.SequenceI;
import java.util.ArrayList;
import java.util.List;
/**
* Assorted methods for analysing or comparing sequences.
*/
public class Comparison
{
private static final int EIGHTY_FIVE = 85;
private static final int TO_UPPER_CASE = 'a' - 'A';
private static final char GAP_SPACE = ' ';
private static final char GAP_DOT = '.';
private static final char GAP_DASH = '-';
public static final String GapChars = new String(new char[] { GAP_SPACE,
GAP_DOT, GAP_DASH });
/**
* DOCUMENT ME!
*
* @param ii
* DOCUMENT ME!
* @param jj
* DOCUMENT ME!
*
* @return DOCUMENT ME!
*/
public static final float compare(SequenceI ii, SequenceI jj)
{
return Comparison.compare(ii, jj, 0, ii.getLength() - 1);
}
/**
* this was supposed to be an ungapped pid calculation
*
* @param ii
* SequenceI
* @param jj
* SequenceI
* @param start
* int
* @param end
* int
* @return float
*/
public static float compare(SequenceI ii, SequenceI jj, int start, int end)
{
String si = ii.getSequenceAsString();
String sj = jj.getSequenceAsString();
int ilen = si.length() - 1;
int jlen = sj.length() - 1;
while (Comparison.isGap(si.charAt(start + ilen)))
{
ilen--;
}
while (Comparison.isGap(sj.charAt(start + jlen)))
{
jlen--;
}
int count = 0;
int match = 0;
float pid = -1;
if (ilen > jlen)
{
for (int j = 0; j < jlen; j++)
{
if (si.substring(start + j, start + j + 1).equals(
sj.substring(start + j, start + j + 1)))
{
match++;
}
count++;
}
pid = (float) match / (float) ilen * 100;
}
else
{
for (int j = 0; j < jlen; j++)
{
if (si.substring(start + j, start + j + 1).equals(
sj.substring(start + j, start + j + 1)))
{
match++;
}
count++;
}
pid = (float) match / (float) jlen * 100;
}
return pid;
}
/**
* this is a gapped PID calculation
*
* @param s1
* SequenceI
* @param s2
* SequenceI
* @return float
*/
public final static float PID(String seq1, String seq2)
{
return PID(seq1, seq2, 0, seq1.length());
}
static final int caseShift = 'a' - 'A';
// Another pid with region specification
public final static float PID(String seq1, String seq2, int start, int end)
{
return PID(seq1, seq2, start, end, true, false);
}
/**
* Calculate percent identity for a pair of sequences over a particular range,
* with different options for ignoring gaps.
*
* @param seq1
* @param seq2
* @param start
* - position in seqs
* @param end
* - position in seqs
* @param wcGaps
* - if true - gaps match any character, if false, do not match
* anything
* @param ungappedOnly
* - if true - only count PID over ungapped columns
* @return
*/
public final static float PID(String seq1, String seq2, int start,
int end, boolean wcGaps, boolean ungappedOnly)
{
int s1len = seq1.length();
int s2len = seq2.length();
int len = Math.min(s1len, s2len);
if (end < len)
{
len = end;
}
if (len < start)
{
start = len - 1; // we just use a single residue for the difference
}
int elen = len - start, bad = 0;
char chr1;
char chr2;
boolean agap;
for (int i = start; i < len; i++)
{
chr1 = seq1.charAt(i);
chr2 = seq2.charAt(i);
agap = isGap(chr1) || isGap(chr2);
if ('a' <= chr1 && chr1 <= 'z')
{
// TO UPPERCASE !!!
// Faster than toUpperCase
chr1 -= caseShift;
}
if ('a' <= chr2 && chr2 <= 'z')
{
// TO UPPERCASE !!!
// Faster than toUpperCase
chr2 -= caseShift;
}
if (chr1 != chr2)
{
if (agap)
{
if (ungappedOnly)
{
elen--;
}
else if (!wcGaps)
{
bad++;
}
}
else
{
bad++;
}
}
}
if (elen < 1)
{
return 0f;
}
return ((float) 100 * (elen - bad)) / elen;
}
/**
* Answers true if the supplied character is a recognised gap character, else
* false. Currently hard-coded to recognise '-', '-' or ' ' (hyphen / dot /
* space).
*
* @param c
*
* @return
*/
public static final boolean isGap(char c)
{
return (c == GAP_DASH || c == GAP_DOT || c == GAP_SPACE) ? true : false;
}
/**
* Answers true if more than 85% of the sequence residues (ignoring gaps) are
* A, G, C, T or U, else false. This is just a heuristic guess and may give a
* wrong answer (as AGCT are also amino acid codes).
*
* @param seqs
* @return
*/
public static final boolean isNucleotide(SequenceI[] seqs)
{
if (seqs == null)
{
return false;
}
char[][] letters = new char[seqs.length][];
for (int i = 0; i < seqs.length; i++)
{
if (seqs[i] != null)
{
char[] sequence = seqs[i].getSequence();
if (sequence != null)
{
letters[i] = sequence;
}
}
}
return areNucleotide(letters);
}
/**
* Answers true if more than 85% of the sequence residues (ignoring gaps) are
* A, G, C, T or U, else false. This is just a heuristic guess and may give a
* wrong answer (as AGCT are also amino acid codes).
*
* @param letters
* @return
*/
static final boolean areNucleotide(char[][] letters)
{
int ntCount = 0;
int aaCount = 0;
for (char[] seq : letters)
{
if (seq == null)
{
continue;
}
// TODO could possibly make an informed guess just from the first sequence
// to save a lengthy calculation
for (char c : seq)
{
if (isNucleotide(c))
{
ntCount++;
}
else if (!isGap(c))
{
aaCount++;
}
}
}
/*
* Check for nucleotide count > 85% of total count (in a form that evades
* int / float conversion or divide by zero).
*/
if (ntCount * 100 > EIGHTY_FIVE * (ntCount + aaCount))
{
return true;
}
else
{
return false;
}
}
/**
* Answers true if the character is one of aAcCgGtTuU
*
* @param c
* @return
*/
public static boolean isNucleotide(char c)
{
if ('a' <= c && c <= 'z')
{
c -= TO_UPPER_CASE;
}
switch (c)
{
case 'A':
case 'C':
case 'G':
case 'T':
case 'U':
return true;
}
return false;
}
/**
* Answers true if every character in the string is one of aAcCgGtTuU, or
* (optionally) a gap character (dot, dash, space), else false
*
* @param s
* @param allowGaps
* @return
*/
public static boolean isNucleotideSequence(String s, boolean allowGaps)
{
if (s == null)
{
return false;
}
for (int i = 0; i < s.length(); i++)
{
char c = s.charAt(i);
if (!isNucleotide(c))
{
if (!allowGaps || !isGap(c))
{
return false;
}
}
}
return true;
}
/**
* Convenience overload of isNucleotide
*
* @param seqs
* @return
*/
public static boolean isNucleotide(SequenceI[][] seqs)
{
if (seqs == null)
{
return false;
}
List flattened = new ArrayList();
for (SequenceI[] ss : seqs)
{
for (SequenceI s : ss)
{
flattened.add(s);
}
}
final SequenceI[] oneDArray = flattened.toArray(new SequenceI[flattened
.size()]);
return isNucleotide(oneDArray);
}
}