package jalview.ws; import java.util.Enumeration; import java.util.Vector; import org.biojava.dasobert.das2.Das2Source; import org.biojava.dasobert.dasregistry.Das1Source; import org.biojava.dasobert.dasregistry.DasCoordinateSystem; import org.biojava.dasobert.dasregistry.DasSource; import jalview.datamodel.Alignment; import jalview.datamodel.AlignmentI; import jalview.datamodel.DBRefSource; import jalview.datamodel.SequenceI; import jalview.ws.seqfetcher.ASequenceFetcher; import jalview.ws.seqfetcher.DbSourceProxy; /** * This is the the concrete implementation of the sequence retrieval interface * and abstract class in jalview.ws.seqfetcher. This implements the run-time * discovery of sequence database clients, and provides a hardwired main for testing all registered handlers. * */ public class SequenceFetcher extends ASequenceFetcher { /** * Thread safe construction of database proxies TODO: extend to a configurable * database plugin mechanism where classes are instantiated by reflection and * queried for their DbRefSource and version association. * */ public SequenceFetcher() { addDBRefSourceImpl(jalview.ws.dbsources.EmblSource.class); addDBRefSourceImpl(jalview.ws.dbsources.EmblCdsSouce.class); addDBRefSourceImpl(jalview.ws.dbsources.Uniprot.class); addDBRefSourceImpl(jalview.ws.dbsources.UnprotName.class); addDBRefSourceImpl(jalview.ws.dbsources.Pdb.class); addDBRefSourceImpl(jalview.ws.dbsources.Pfam.class); registerDasSequenceSources(); } /** * simple run method to test dbsources. * @param argv */ public static void main(String[] argv) { AlignmentI ds = null; Vector noProds = new Vector(); String usage = "SequenceFetcher.main [ ]\n" +"With no arguments, all DbSources will be queried with their test Accession number.\n" +"If given two arguments, SequenceFetcher will try to find the DbFetcher corresponding to and retrieve from it."; if (argv != null && argv.length > 0) { DbSourceProxy sp = new SequenceFetcher().getSourceProxy(argv[0]); if (sp!=null) { AlignmentI al = null; try { al = sp.getSequenceRecords(argv[1]); } catch (Exception e) { e.printStackTrace(); System.err.println("Error when retrieving "+argv[1]+" from "+argv[0]+"\nUsage: "+usage); } SequenceI[] prod = al.getSequencesArray(); if (al!=null) { for (int p = 0; p < prod.length; p++) { System.out.println("Prod " + p + ": " + prod[p].getDisplayId(true) + " : "+prod[p].getDescription()); } } return; } else { System.err.println("Can't resolve "+argv[0]+" as a database name. Allowed values are :\n"+new SequenceFetcher().getSupportedDb()); } System.out .println(usage); } ASequenceFetcher sfetcher = new SequenceFetcher(); String[] dbSources = sfetcher.getSupportedDb(); for (int dbsource=0; dbsource 0 && sp.getDbSourceProperties()!=null) { boolean dna = sp.getDbSourceProperties().containsKey( DBRefSource.DNACODINGSEQDB) || sp.getDbSourceProperties().containsKey( DBRefSource.DNASEQDB) || sp.getDbSourceProperties().containsKey( DBRefSource.CODINGSEQDB); // try and find products String types[] = jalview.analysis.CrossRef.findSequenceXrefTypes( dna, al.getSequencesArray()); if (types != null) { System.out.println("Xref Types for: " + (dna ? "dna" : "prot")); for (int t = 0; t < types.length; t++) { System.out.println("Type: " + types[t]); SequenceI[] prod = jalview.analysis.CrossRef .findXrefSequences(al.getSequencesArray(), dna, types[t]).getSequencesArray(); System.out.println("Found " + ((prod == null) ? "no" : "" + prod.length) + " products"); if (prod != null) { for (int p = 0; p < prod.length; p++) { System.out.println("Prod " + p + ": " + prod[p].getDisplayId(true)); } } } } else { noProds.addElement((dna ? new Object[] { al, al } : new Object[] { al })); } } } catch (Exception ex) { System.out.println("ERROR:Failed to retrieve test query."); ex.printStackTrace(System.out); } if (al == null) { System.out.println("ERROR:No alignment retrieved."); StringBuffer raw = sp.getRawRecords(); if (raw != null) System.out.println(raw.toString()); else System.out.println("ERROR:No Raw results."); } else { System.out.println("Retrieved " + al.getHeight() + " sequences."); for (int s = 0; s < al.getHeight(); s++) { SequenceI sq = al.getSequenceAt(s); while (sq.getDatasetSequence() != null) { sq = sq.getDatasetSequence(); } if (ds == null) { ds = new Alignment(new SequenceI[] { sq }); } else { ds.addSequence(sq); } } } System.out.flush(); System.err.flush(); } if (noProds.size() > 0) { Enumeration ts = noProds.elements(); while (ts.hasMoreElements()) { Object[] typeSq = (Object[]) ts.nextElement(); boolean dna = (typeSq.length > 1); AlignmentI al = (AlignmentI) typeSq[0]; System.out.println("Trying getProducts for " + al.getSequenceAt(0).getDisplayId(true)); System.out.println("Search DS Xref for: " + (dna ? "dna" : "prot")); // have a bash at finding the products amongst all the retrieved // sequences. SequenceI[] seqs = al.getSequencesArray(); Alignment prodal = jalview.analysis.CrossRef.findXrefSequences( seqs, dna, null, ds); System.out.println("Found " + ((prodal == null) ? "no" : "" + prodal.getHeight()) + " products"); if (prodal != null) { SequenceI[] prod = prodal.getSequencesArray(); // note // should // test // rather // than // throw // away // codon // mapping // (if // present) for (int p = 0; p < prod.length; p++) { System.out.println("Prod " + p + ": " + prod[p].getDisplayId(true)); } } } } } /** * query the currently defined DAS source registry for sequence sources and add a DasSequenceSource instance for each source to the SequenceFetcher source list. */ public void registerDasSequenceSources() { DasSource[] sources = jalview.ws.DasSequenceFeatureFetcher.getDASSources(); for (int s=0;s