package fr.orsay.lri.varna.models.treealign; import java.util.ArrayList; import java.util.LinkedList; import java.util.List; import fr.orsay.lri.varna.exceptions.MappingException; import fr.orsay.lri.varna.models.rna.Mapping; import fr.orsay.lri.varna.models.rna.RNA; /** * This class contains all functions that are specific to trees * (class Tree) of RNA, with RNANodeValue2. * * @author Raphael Champeimont * */ public class RNATree2 { /** * Convert an RNA object into a RNA tree with RNANodeValue2. * @throws RNATree2Exception */ public static Tree RNATree2FromRNA(RNA rna) throws RNATree2Exception { Tree fullTree = RNATree.RNATreeFromRNA(rna); return RNATree2FromRNATree(fullTree); } /** * Convert from RNANodeValue model to RNANodeValue2 model, * ie. compact consecutive non-paired bases. */ public static Tree RNATree2FromRNATree(Tree originalTree) throws RNATree2Exception { Tree newTree = new Tree(); // Root in original tree is fake, so make a fake root newTree.setValue(null); newTree.replaceChildrenListBy(RNAForest2FromRNAForest(originalTree.getChildren())); return newTree; } private static void RNAForest2FromRNAForestCommitNonPaired(List> forest, List consecutiveNonPairedBases) { // add the group of non-paired bases if there is one if (consecutiveNonPairedBases.size() > 0) { RNANodeValue2 groupOfConsecutiveBases = new RNANodeValue2(false); groupOfConsecutiveBases.getNodes().addAll(consecutiveNonPairedBases); Tree groupOfConsecutiveBasesNode = new Tree(); groupOfConsecutiveBasesNode.setValue(groupOfConsecutiveBases); forest.add(groupOfConsecutiveBasesNode); consecutiveNonPairedBases.clear(); } } private static List> RNAForest2FromRNAForest(List> originalForest) throws RNATree2Exception { List> forest = new ArrayList>(); List consecutiveNonPairedBases = new LinkedList(); for (Tree originalTree: originalForest) { if (originalTree.getValue().getRightBasePosition() == -1) { // non-paired base if (originalTree.getChildren().size() > 0) { throw (new RNATree2Exception("Non-paired base cannot have children.")); } switch (originalTree.getValue().getOrigin()) { case BASE_FROM_HELIX_STRAND5: case BASE_FROM_HELIX_STRAND3: // This base is part of a broken base pair // if we have gathered some non-paired bases, add a node with // the group of them RNAForest2FromRNAForestCommitNonPaired(forest, consecutiveNonPairedBases); // now add the node RNANodeValue2 pairedBase = new RNANodeValue2(true); pairedBase.setNode(originalTree.getValue()); Tree pairedBaseNode = new Tree(); pairedBaseNode.setValue(pairedBase); forest.add(pairedBaseNode); break; case BASE_FROM_UNPAIRED_REGION: consecutiveNonPairedBases.add(originalTree.getValue()); break; case BASE_PAIR_FROM_HELIX: throw (new RNATree2Exception("Origin is BASE_PAIR_FROM_HELIX but this is not a pair.")); } } else { // paired bases // if we have gathered some non-paired bases, add a node with // the group of them RNAForest2FromRNAForestCommitNonPaired(forest, consecutiveNonPairedBases); // now add the node RNANodeValue2 pairedBase = new RNANodeValue2(true); pairedBase.setNode(originalTree.getValue()); Tree pairedBaseNode = new Tree(); pairedBaseNode.setValue(pairedBase); pairedBaseNode.replaceChildrenListBy(RNAForest2FromRNAForest(originalTree.getChildren())); forest.add(pairedBaseNode); } } // if there we have some non-paired bases, add them grouped RNAForest2FromRNAForestCommitNonPaired(forest, consecutiveNonPairedBases); return forest; } /** * Convert an RNA tree (with RNANodeValue2) alignment into a Mapping. */ public static Mapping mappingFromAlignment(Tree> alignment) throws MappingException { ConvertToMapping converter = new ConvertToMapping(); return converter.convert(alignment); } private static class ConvertToMapping { private Mapping m; ExampleDistance3 sequenceAligner = new ExampleDistance3(); public Mapping convert(Tree> tree) throws MappingException { m = new Mapping(); convertSubTree(tree); return m; } private void convertSubTree(Tree> tree) throws MappingException { AlignedNode alignedNode = tree.getValue(); Tree leftNode = alignedNode.getLeftNode(); Tree rightNode = alignedNode.getRightNode(); if (leftNode != null && rightNode != null) { RNANodeValue2 v1 = leftNode.getValue(); RNANodeValue2 v2 = rightNode.getValue(); if (v1.isSingleNode() && v2.isSingleNode()) { // we have aligned (x,y) with (x',y') // so we map x with x' and y with y' RNANodeValue vsn1 = v1.getNode(); RNANodeValue vsn2 = v2.getNode(); int l1 = vsn1.getLeftBasePosition(); int r1 = vsn1.getRightBasePosition(); int l2 = vsn2.getLeftBasePosition(); int r2 = vsn2.getRightBasePosition(); if (l1 >= 0 && l2 >= 0) { m.addCouple(l1, l2); } if (r1 >= 0 && r2 >= 0) { m.addCouple(r1, r2); } } else if (!v1.isSingleNode() && !v2.isSingleNode()) { // We have aligned x1 x2 ... xn with y1 y2 ... ym. // So we will now (re-)compute this sequence alignment. int[][] sequenceAlignment = sequenceAligner.alignSequenceNodes(v1, v2).getAlignment(); int l = sequenceAlignment[0].length; for (int i=0; i> child: tree.getChildren()) { convertSubTree(child); } } } }