/*
* Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
* Copyright (C) $$Year-Rel$$ The Jalview Authors
*
* This file is part of Jalview.
*
* Jalview is free software: you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation, either version 3
* of the License, or (at your option) any later version.
*
* Jalview is distributed in the hope that it will be useful, but
* WITHOUT ANY WARRANTY; without even the implied warranty
* of MERCHANTABILITY or FITNESS FOR A PARTICULAR
* PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Jalview. If not, see .
* The Jalview Authors are detailed in the 'AUTHORS' file.
*/
package jalview.io.gff;
import static org.testng.AssertJUnit.assertEquals;
import static org.testng.AssertJUnit.assertSame;
import static org.testng.AssertJUnit.assertTrue;
import static org.testng.internal.junit.ArrayAsserts.assertArrayEquals;
import jalview.datamodel.AlignedCodonFrame;
import jalview.datamodel.Alignment;
import jalview.datamodel.AlignmentI;
import jalview.datamodel.Mapping;
import jalview.datamodel.Sequence;
import jalview.datamodel.SequenceDummy;
import jalview.datamodel.SequenceI;
import jalview.gui.AlignFrame;
import jalview.gui.JvOptionPane;
import jalview.io.DataSourceType;
import jalview.io.FileLoader;
import java.util.List;
import org.testng.annotations.BeforeClass;
import org.testng.annotations.Test;
/**
* Tests of use cases that include parsing GFF (version 2 or 3) features that
* describe mappings between protein and cDNA. The format of the GFF varies
* depending on which tool generated it.
*/
public class GffTests
{
@BeforeClass(alwaysRun = true)
public void setUpJvOptionPane()
{
JvOptionPane.setInteractiveMode(false);
JvOptionPane.setMockResponse(JvOptionPane.CANCEL_OPTION);
}
/**
* Test the case where we load a protein ('query') sequence, then exonerateGff
* describing its mapping to cDNA, and then a DNA sequence including the
* mapped region
*/
@Test(groups = "Functional")
public void testResolveExonerateGff()
{
String proteinSeq = ">prot1/10-16\nYCWRSGA";
AlignFrame af = new FileLoader(false).LoadFileWaitTillLoaded(
proteinSeq, DataSourceType.PASTE);
/*
* exonerate GFF output mapping residues 11-15 (CWRSG)
* to bases 24-10 in sequence 'dna1' (reverse strand)
*/
String exonerateGff = "##gff-version 2\n"
+ "prot1\tprotein2genome\tsimilarity\t11\t15\t99\t-\t.\talignment_id 0 ; Target dna1 ; Align 11 24 5";
af.loadJalviewDataFile(exonerateGff, DataSourceType.PASTE, null, null);
/*
* check we have a mapping from prot1 to SequenceDummy 'dna1'
*/
AlignmentI dataset = af.getViewport().getAlignment().getDataset();
assertEquals(1, dataset.getSequences().size());
assertEquals("prot1", dataset.getSequenceAt(0).getName());
assertEquals("YCWRSGA", dataset.getSequenceAt(0).getSequenceAsString());
List mappings = dataset.getCodonFrames();
assertEquals(1, mappings.size());
AlignedCodonFrame mapping = mappings.iterator().next();
SequenceI mappedDna = mapping.getDnaForAaSeq(dataset.getSequenceAt(0));
assertTrue(mappedDna instanceof SequenceDummy);
assertEquals("dna1", mappedDna.getName());
Mapping[] mapList = mapping.getProtMappings();
assertEquals(1, mapList.length);
// 11 in protein should map to codon [24, 23, 22] in dna
int[] mappedRegion = mapList[0].getMap().locateInFrom(11, 11);
assertArrayEquals(new int[] { 24, 22 }, mappedRegion);
// 15 in protein should map to codon [12, 11, 10] in dna
mappedRegion = mapList[0].getMap().locateInFrom(15, 15);
assertArrayEquals(new int[] { 12, 10 }, mappedRegion);
SequenceI dna1 = new Sequence("dna1", "AAACCCGGGTTTAAACCCGGGTTT");
AlignmentI al = new Alignment(new SequenceI[] { dna1 });
al.setDataset(null);
/*
* Now 'realise' the virtual mapping to the real DNA sequence;
* interactively this could be by a drag or fetch of the sequence data
* on to the alignment
*/
mapping.realiseWith(dna1);
// verify the mapping is now from the real, not the dummy sequence
assertSame(dna1.getDatasetSequence(),
mapping.getDnaForAaSeq(dataset.getSequenceAt(0)));
}
}