/***************************************************************** * SQUID - a library of functions for biological sequence analysis * Copyright (C) 1992-2002 Washington University School of Medicine * * This source code is freely distributed under the terms of the * GNU General Public License. See the files COPYRIGHT and LICENSE * for details. *****************************************************************/ /* a2m.c * * reading/writing A2M (aligned FASTA) files. * * RCS $Id: a2m.c 242 2011-05-27 14:04:21Z andreas $ (Original squid RCS Id: a2m.c,v 1.1 1999/07/15 22:26:40 eddy Exp) */ #include #include #include #include "squid.h" #include "msa.h" /* Function: ReadA2M() * Date: SRE, Sun Jun 6 17:11:29 1999 [bus from Madison 1999 worm mtg] * * Purpose: Parse an alignment read from an open A2M format * alignment file. A2M is a single alignment format. * Return the alignment, or NULL if we've already * read the alignment. * * Args: afp - open alignment file * * Returns: MSA * - an alignment object. * Caller responsible for an MSAFree() */ MSA * ReadA2M(MSAFILE *afp) { MSA *msa; char *buf; char *name; char *desc; char *seq; int idx; int len1, len2; if (feof(afp->f)) return NULL; name = NULL; msa = MSAAlloc(10, 0); idx = 0; while ((buf = MSAFileGetLine(afp)) != NULL) { if (*buf == '>') { buf++; /* skip the '>' */ if ((name = sre_strtok(&buf, WHITESPACE, &len1)) == NULL) Die("Blank name in A2M file %s (line %d)\n", afp->fname, afp->linenumber); desc = sre_strtok(&buf, "\n", &len2); idx = GKIStoreKey(msa->index, name); if (idx >= msa->nseqalloc) MSAExpand(msa); msa->sqname[idx] = sre_strdup(name, len1); if (desc != NULL) MSASetSeqDescription(msa, idx, desc); msa->nseq++; } else if (name != NULL) { if ((seq = sre_strtok(&buf, WHITESPACE, &len1)) == NULL) continue; msa->sqlen[idx] = sre_strcat(&(msa->aseq[idx]), msa->sqlen[idx], seq, len1); } } if (name == NULL) { MSAFree(msa); return NULL; } MSAVerifyParse(msa); return msa; } /* Function: WriteA2M() * Date: SRE, Sun Jun 6 17:40:35 1999 [bus from Madison, 1999 worm mtg] * * Purpose: Write an "aligned FASTA" (aka a2m, to UCSC) formatted * alignment. * * Args: fp - open FILE to write to. * msa - alignment to write * * Returns: void */ void #ifdef CLUSTALO WriteA2M(FILE *fp, MSA *msa, int vienna) #else WriteA2M(FILE *fp, MSA *msa) #endif { int idx; /* sequence index */ int pos; /* position in sequence */ char buf[64]; /* buffer for individual lines */ int cpl = 60; /* char per line; must be < 64 unless buf is bigger */ buf[cpl] = '\0'; for (idx = 0; idx < msa->nseq; idx++) { #ifdef CLUSTALO /* most fasta sequences don't have a description, which * leads to a trailing white space in the original code */ fprintf(fp, ">%s", msa->sqname[idx]); if (msa->sqdesc != NULL && msa->sqdesc[idx] != NULL && !vienna) { fprintf(fp, " %s", msa->sqdesc[idx]); } fprintf(fp, "\n"); #else fprintf(fp, ">%s %s\n", msa->sqname[idx], (msa->sqdesc != NULL && msa->sqdesc[idx] != NULL) ? msa->sqdesc[idx] : ""); #endif for (pos = 0; pos < msa->alen; pos+=cpl) { strncpy(buf, &(msa->aseq[idx][pos]), cpl); if (vienna) fprintf(fp, "%s", buf); else fprintf(fp, "%s\n", buf); } if (vienna) fprintf(fp, "\n"); } }