<title>Sequence Features File</title>
</head>
<body>
-<p><strong>Sequence Features File</strong><p>
-<p>
-The Sequence features file (which used to be known as the
-"Groups file" prior to version 2.08) is a simple way of getting
-your own sequence annotations into Jalview. It was introduced to
-allow sequence features to be rendered in the Jalview applet, and
-so is intentionally lightweight and minimal because the applet is
-often used in situations where data file size must be kept to a
-minimum, and no XML parser is available.</p>
-
-<p>Features files are imported into Jalview in the following
-ways:<br>
-
-<ul>
-<li>from the command line
-
-<pre>
+ <p>
+ <strong>Sequence Features File</strong>
+ <p>
+ <p>The Sequence features file (which used to be known as the
+ "Groups file" prior to version 2.08) is a simple way of getting your
+ own sequence annotations into Jalview. It was introduced to allow
+ sequence features to be rendered in the Jalview applet, and so is
+ intentionally lightweight and minimal because the applet is often
+ used in situations where data file size must be kept to a minimum,
+ and no XML parser is available.</p>
+
+ <p>
+ Features files are imported into Jalview in the following ways:<br>
+ <ul>
+ <li>from the command line <pre>
<strong> -features <<em>Features filename</em>></strong>
</pre>
-</li>
-
-<li>Dragging a features file onto an alignment window</li>
-
-<li>Via the "Load Features / Annotations" entry in the
-<strong>File</strong> menu of an alignment window.</li>
-</ul>
-
-</p>
-
-<p><strong>Sequence Features File Format</strong></p>
-<p>
-A features file is a simple ASCII text file, where each line
-contains tab separated text fields. <strong>No comments are
-allowed</strong>.</p>
-<p>
-The first set of lines contain type definitions:
-
-<pre>
+ </li>
+
+ <li>Dragging a features file onto an alignment window</li>
+
+ <li>Via the "Load Features / Annotations" entry in
+ the <strong>File</strong> menu of an alignment window.
+ </li>
+ </ul>
+
+ </p>
+
+ <p>
+ <strong>Sequence Features File Format</strong>
+ </p>
+ <p>
+ A features file is a simple ASCII text file, where each line
+ contains tab separated text fields. <strong>No comments are
+ allowed</strong>.
+ </p>
+ <p>The first set of lines contain type definitions:
+ <pre>
<strong><em>Feature label</em>	<em>Feature Colour</em>
<!-- 	<em>Feature links</em> --></strong>
</pre>
-A feature type has a text label, and a colour specification. This
-can be either:
+ A feature type has a text label, and a colour specification. This can
+ be either:
-<ul>
-<li>A single colour specified as either a red,green,blue 24 bit
-triplet in hexadecimal (eg. 00ff00) or as comma separated numbers
-(ranging from 0 to 255))</li>
+ <ul>
+ <li>A single colour specified as either a red,green,blue 24 bit
+ triplet in hexadecimal (eg. 00ff00) or as comma separated numbers
+ (ranging from 0 to 255))</li>
-<li>A <a href="featureschemes.html">graduated colourscheme</a>
-specified as a "|" separated list of fields:
-
-<pre>
+ <li>A <a href="featureschemes.html">graduated colourscheme</a>
+ specified as a "|" separated list of fields: <pre>
[label|]<mincolor>|<maxcolor>|[absolute|]<minvalue>|<maxvalue>[|<thresholdtype>|[<threshold value>]]
-</pre>
-
-The fields are as follows:
-
-<ul>
-<li><em>label</em><br>
- Indicate that the feature description should be used to create a
-colour for features of this type.<br>
-<em>Note: if no threshold value is needed then the final '|' may be
-ommitted.<br>
-This keyword was added in Jalview 2.6</em></li>
-
-<li><em>mincolor</em> and <em>maxcolor</em><br>
- Colour triplets specified as hexadecimal or comma separated values
-(may be left blank for a <em>label</em> style colourscheme, but
-remember to specify the remaining fields)</li>
-
-<li><em>absolute</em><br>
- An optional switch indicating that the <em>minvalue</em> and
-<em>maxvalue</em> parameters should be left as is, rather than
-rescaled according to the range of scores for this feature
-type.</li>
-
-<li><em>minvalue</em> and <em>maxvalue</em><br>
- Minimum and maximum values defining the range of scores for which
-the colour range will be defined over. If minvalue is greater than
-maxvalue then the linear mapping will have negative gradient.</li>
-
-<li><em>thresholdtype</em><br>
- Either "none", "below", or "above". <em>below</em> and
-<em>above</em> require an additional <em>threshold value</em> which
-is used to control the display of features with a score either
-below or above the value.</li>
-</ul>
-</li>
-</ul>
-</p>
-
-<p>The remaining lines in the file are the sequence annotation
-definitions, where the now defined features are attached to regions
-on particular sequences. Each feature can optionally include some descriptive text
-which is displayed in a tooltip when the mouse is near the feature on that
-sequence (and can also be used to generate a colour the feature).</p>
-
-<p>If your sequence annotation is already available in GFF Format (see <a href="http://www.sanger.ac.uk/resources/software/gff/spec.html">http://www.sanger.ac.uk/resources/software/gff/spec.html</a>),
-then you can leave it as is, after first adding a line containing
-only 'GFF' after any Jalview feature colour definitions (<em>this mixed format capability was added in Jalview 2.6</em>). Alternately, you can use Jalview's own sequence feature
-annotation format, which additionally allows HTML and URLs to be
-directly attached to each piece of annotation.</p>
-
-<p><strong>Jalview's sequence feature annotation format</strong></p>
-<p>
-Each feature is specified as a tab-separated series of columns as defined below:
-<pre>
+</pre> The fields are as follows:
+
+ <ul>
+ <li><em>label</em><br> Indicate that the feature
+ description should be used to create a colour for features of
+ this type.<br> <em>Note: if no threshold value is
+ needed then the final '|' may be omitted.<br> This
+ keyword was added in Jalview 2.6
+ </em></li>
+
+ <li><em>mincolor</em> and <em>maxcolor</em><br> Colour
+ triplets specified as hexadecimal or comma separated values
+ (may be left blank for a <em>label</em> style colourscheme,
+ but remember to specify the remaining fields)</li>
+
+ <li><em>absolute</em><br> An optional switch
+ indicating that the <em>minvalue</em> and <em>maxvalue</em>
+ parameters should be left as is, rather than rescaled
+ according to the range of scores for this feature type.</li>
+
+ <li><em>minvalue</em> and <em>maxvalue</em><br>
+ Minimum and maximum values defining the range of scores for
+ which the colour range will be defined over. If minvalue is
+ greater than maxvalue then the linear mapping will have
+ negative gradient.</li>
+
+ <li><em>thresholdtype</em><br> Either
+ "none", "below", or "above". <em>below</em>
+ and <em>above</em> require an additional <em>threshold
+ value</em> which is used to control the display of features with
+ a score either below or above the value.</li>
+ </ul>
+ </li>
+ </ul>
+ </p>
+
+ <p>The remaining lines in the file are the sequence annotation
+ definitions, where the now defined features are attached to regions
+ on particular sequences. Each feature can optionally include some
+ descriptive text which is displayed in a tooltip when the mouse is
+ near the feature on that sequence (and can also be used to generate
+ a colour the feature).</p>
+
+ <p>
+ If your sequence annotation is already available in <a href="http://gmod.org/wiki/GFF2">GFF2</a> (http://gmod.org/wiki/GFF2) or
+ <a href="https://github.com/The-Sequence-Ontology/Specifications/blob/master/gff3.md">GFF3</a>
+ (http://github.com/The-Sequence-Ontology/Specifications/blob/master/gff3.md) format,
+ then you can leave it as is, after first adding a line containing only
+ 'GFF' after any Jalview feature colour definitions (<em>this
+ mixed format capability was added in Jalview 2.6</em>). Alternately,
+ you can use Jalview's own sequence feature annotation format, which
+ additionally allows HTML and URLs to be directly attached to each
+ piece of annotation.
+ </p>
+
+ <p>
+ <strong>Jalview's sequence feature annotation format</strong>
+ </p>
+ <p>Each feature is specified as a tab-separated series of columns
+ as defined below:
+ <pre>
<em>description</em>	<em>sequenceId</em>	<em>sequenceIndex</em>	<em>start</em>	<em>end</em>	<em>featureType</em>	<em>score (optional)</em>
</pre>
-This format allows two alternate ways of referring to a sequence, either by
-its text ID, or its index in an associated alignment. Normally, sequence features are associated with sequences rather
-than alignments, and the sequenceIndex field is given as "-1". In
-order to specify a sequence by its index in a particular alignment,
-the sequenceId should be given as "ID_NOT_SPECIFIED", otherwise the
-sequenceId field will be used in preference to the sequenceIndex
-field.
-</p>
-
-
-<p>The description may contain simple HTML document body tags if
-enclosed by "<html></html>" and these will be rendered
-as formatted tooltips in the Jalview Application (the Jalview
-applet is not capable of rendering HTML tooltips, so all formatting
-tags will be removed).<br>
-<em>Attaching Links to Sequence Features</em><br>
-Any anchor tags in an html formatted description line will be
-translated into URL links. A link symbol will be displayed adjacent
-to any feature which includes links, and these are made available
-from the <a href="../menus/popupMenu.html#sqid.popup">links
-submenu</a> of the popup menu which is obtained by right-clicking
-when a link symbol is displayed in the tooltip.<br>
-<em>Non-positional features</em><br>
-Specify the <em>start</em> and <em>end</em> for a feature to be
-<strong>0</strong> in order to attach it to the whole sequence.
-Non-positional features are shown in a tooltip when the mouse
-hovers over the sequence ID panel, and any embedded links can be
-accessed from the popup menu.<br/> <em>Scores</em><br>
-Scores can be associated with sequence features, and used to sort
-sequences or shade the alignment (this was added in Jalview 2.5).
-The score field is optional, and malformed scores will be
-ignored.</p>
-
-<p>Feature annotations can be collected into named groups by
-prefixing definitions with lines of the form:
-
-<pre>
+ This format allows two alternate ways of referring to a sequence,
+ either by its text ID, or its index (base 0) in an associated
+ alignment. Normally, sequence features are associated with sequences
+ rather than alignments, and the sequenceIndex field is given as
+ "-1". In order to specify a sequence by its index in a
+ particular alignment, the sequenceId should be given as
+ "ID_NOT_SPECIFIED", otherwise the sequenceId field will be
+ used in preference to the sequenceIndex field.
+ </p>
+
+
+ <p>
+ The description may contain simple HTML document body tags if
+ enclosed by "<html></html>" and these will be
+ rendered as formatted tooltips in the Jalview Application (the
+ Jalview applet is not capable of rendering HTML tooltips, so all
+ formatting tags will be removed).<br> <em>Attaching Links
+ to Sequence Features</em><br> Any anchor tags in an html formatted
+ description line will be translated into URL links. A link symbol
+ will be displayed adjacent to any feature which includes links, and
+ these are made available from the <a
+ href="../menus/popupMenu.html#sqid.popup">links submenu</a>
+ of the popup menu which is obtained by right-clicking when a link
+ symbol is displayed in the tooltip.<br> <em>Non-positional
+ features</em><br> Specify the <em>start</em> and <em>end</em> for
+ a feature to be <strong>0</strong> in order to attach it to the
+ whole sequence. Non-positional features are shown in a tooltip when
+ the mouse hovers over the sequence ID panel, and any embedded links
+ can be accessed from the popup menu.<br /> <em>Scores</em><br>
+ Scores can be associated with sequence features, and used to sort
+ sequences or shade the alignment (this was added in Jalview 2.5).
+ The score field is optional, and malformed scores will be ignored.
+ </p>
+
+ <p>Feature annotations can be collected into named groups by
+ prefixing definitions with lines of the form:
+ <pre>
<strong>startgroup groupname</strong>
</pre>
-.. and subsequently post-fixing the group with:
+ .. and subsequently post-fixing the group with:
-<pre>
+ <pre>
<strong>endgroup groupname</strong>
</pre>
-Feature grouping was introduced in version 2.08, and used to
-control whether a set of features are either hidden or shown
-together in the <a href="seqfeatures.html">sequence Feature
-settings dialog box</a>.</p>
-
+ Feature grouping was introduced in version 2.08, and used to control
+ whether a set of features are either hidden or shown together in the
+ <a href="seqfeatures.html">sequence Feature settings dialog box</a>.
+ </p>
+
-<p>A complete example is shown below :
-<pre>
+ <p>A complete example is shown below :
+ <pre>
domain	red
metal ion-binding site	00ff00
transit peptide	0,105,215