X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=binaries%2Fsrc%2Fmafft%2Fcore%2Ftbfast.c;fp=binaries%2Fsrc%2Fmafft%2Fcore%2Ftbfast.c;h=6acce2877ecc5c22c97d6d8eacec4ee5bfdbe755;hb=063b30bb5e8161134ae764742636ab538e10eea7;hp=0000000000000000000000000000000000000000;hpb=6e0ce943f09b5ac30f3eb8dc0f20bc75114669ce;p=jabaws.git

diff --git a/binaries/src/mafft/core/tbfast.c b/binaries/src/mafft/core/tbfast.c
new file mode 100644
index 0000000..6acce28
--- /dev/null
+++ b/binaries/src/mafft/core/tbfast.c
@@ -0,0 +1,1880 @@
+#include "mltaln.h"
+
+#define DEBUG 0
+#define IODEBUG 0
+#define SCOREOUT 0
+
+static int nadd;
+static int treein;
+static int topin;
+static int treeout;
+static int distout;
+static int noalign;
+
+typedef struct _jobtable
+{
+    int i;  
+    int j;  
+} Jobtable;
+
+#ifdef enablemultithread
+typedef struct _distancematrixthread_arg
+{
+	int njob;
+	int thread_no;
+	float *selfscore;
+	float **iscore;
+	char **seq;
+	Jobtable *jobpospt;
+	pthread_mutex_t *mutex;
+} distancematrixthread_arg_t;
+
+typedef struct _treebasethread_arg
+{
+	int thread_no;
+	int *nrunpt;
+	int njob;
+	int *nlen;
+	int *jobpospt;
+	int ***topol;
+	Treedep *dep;
+	char **aseq;
+	double *effarr;
+	int *alloclenpt;
+	LocalHom **localhomtable;
+	RNApair ***singlerna;
+	double *effarr_kozo;
+	int *fftlog;
+	pthread_mutex_t *mutex;
+	pthread_cond_t *treecond;
+} treebasethread_arg_t;
+#endif
+
+void arguments( int argc, char *argv[] )
+{
+    int c;
+
+	nthread = 1;
+	outnumber = 0;
+	scoreout = 0;
+	treein = 0;
+	topin = 0;
+	rnaprediction = 'm';
+	rnakozo = 0;
+	nevermemsave = 0;
+	inputfile = NULL;
+	addfile = NULL;
+	addprofile = 1;
+	fftkeika = 0;
+	constraint = 0;
+	nblosum = 62;
+	fmodel = 0;
+	calledByXced = 0;
+	devide = 0;
+	use_fft = 0; // chuui
+	force_fft = 0;
+	fftscore = 1;
+	fftRepeatStop = 0;
+	fftNoAnchStop = 0;
+    weight = 3;
+    utree = 1;
+	tbutree = 1;
+    refine = 0;
+    check = 1;
+    cut = 0.0;
+    disp = 0;
+    outgap = 1;
+    alg = 'A';
+    mix = 0;
+	tbitr = 0;
+	scmtd = 5;
+	tbweight = 0;
+	tbrweight = 3;
+	checkC = 0;
+	treemethod = 'X';
+	contin = 0;
+	scoremtx = 1;
+	kobetsubunkatsu = 0;
+	dorp = NOTSPECIFIED;
+	ppenalty = NOTSPECIFIED;
+	ppenalty_ex = NOTSPECIFIED;
+	poffset = NOTSPECIFIED;
+	kimuraR = NOTSPECIFIED;
+	pamN = NOTSPECIFIED;
+	geta2 = GETA2;
+	fftWinSize = NOTSPECIFIED;
+	fftThreshold = NOTSPECIFIED;
+	RNAppenalty = NOTSPECIFIED;
+	RNAppenalty_ex = NOTSPECIFIED;
+	RNApthr = NOTSPECIFIED;
+	TMorJTT = JTT;
+	consweight_multi = 1.0;
+	consweight_rna = 0.0;
+
+    while( --argc > 0 && (*++argv)[0] == '-' )
+	{
+        while ( ( c = *++argv[0] ) )
+		{
+            switch( c )
+            {
+				case 'i':
+					inputfile = *++argv;
+					fprintf( stderr, "inputfile = %s\n", inputfile );
+					--argc;
+                    goto nextoption;
+				case 'I':
+					nadd = atoi( *++argv );
+					fprintf( stderr, "nadd = %d\n", nadd );
+					--argc;
+					goto nextoption;
+				case 'e':
+					RNApthr = (int)( atof( *++argv ) * 1000 - 0.5 );
+					--argc;
+					goto nextoption;
+				case 'o':
+					RNAppenalty = (int)( atof( *++argv ) * 1000 - 0.5 );
+					--argc;
+					goto nextoption;
+				case 'f':
+					ppenalty = (int)( atof( *++argv ) * 1000 - 0.5 );
+//					fprintf( stderr, "ppenalty = %d\n", ppenalty );
+					--argc;
+					goto nextoption;
+				case 'g':
+					ppenalty_ex = (int)( atof( *++argv ) * 1000 - 0.5 );
+					fprintf( stderr, "ppenalty_ex = %d\n", ppenalty_ex );
+					--argc;
+					goto nextoption;
+				case 'h':
+					poffset = (int)( atof( *++argv ) * 1000 - 0.5 );
+//					fprintf( stderr, "poffset = %d\n", poffset );
+					--argc;
+					goto nextoption;
+				case 'k':
+					kimuraR = atoi( *++argv );
+					fprintf( stderr, "kappa = %d\n", kimuraR );
+					--argc;
+					goto nextoption;
+				case 'b':
+					nblosum = atoi( *++argv );
+					scoremtx = 1;
+					fprintf( stderr, "blosum %d / kimura 200\n", nblosum );
+					--argc;
+					goto nextoption;
+				case 'j':
+					pamN = atoi( *++argv );
+					scoremtx = 0;
+					TMorJTT = JTT;
+					fprintf( stderr, "jtt/kimura %d\n", pamN );
+					--argc;
+					goto nextoption;
+				case 'm':
+					pamN = atoi( *++argv );
+					scoremtx = 0;
+					TMorJTT = TM;
+					fprintf( stderr, "tm %d\n", pamN );
+					--argc;
+					goto nextoption;
+				case 'l':
+					fastathreshold = atof( *++argv );
+					constraint = 2;
+					--argc;
+					goto nextoption;
+				case 'r':
+					consweight_rna = atof( *++argv );
+					rnakozo = 1;
+					--argc;
+					goto nextoption;
+				case 'c':
+					consweight_multi = atof( *++argv );
+					--argc;
+					goto nextoption;
+				case 'C':
+					nthread = atoi( *++argv );
+					fprintf( stderr, "nthread = %d\n", nthread );
+					--argc; 
+					goto nextoption;
+				case 'R':
+					rnaprediction = 'r';
+					break;
+				case 's':
+					RNAscoremtx = 'r';
+					break;
+#if 1
+				case 'a':
+					fmodel = 1;
+					break;
+#endif
+				case 'K':
+					addprofile = 0;
+					break;
+				case 'y':
+					distout = 1;
+					break;
+				case 't':
+					treeout = 1;
+					break;
+				case 'T':
+					noalign = 1;
+					break;
+				case 'D':
+					dorp = 'd';
+					break;
+				case 'P':
+					dorp = 'p';
+					break;
+#if 1
+				case 'O':
+					outgap = 0;
+					break;
+#else
+				case 'O':
+					fftNoAnchStop = 1;
+					break;
+#endif
+				case 'S':
+					scoreout = 1;
+					break;
+#if 0
+				case 'e':
+					fftscore = 0;
+					break;
+				case 'r':
+					fmodel = -1;
+					break;
+				case 'R':
+					fftRepeatStop = 1;
+					break;
+				case 's':
+					treemethod = 's';
+					break;
+#endif
+				case 'X':
+					treemethod = 'X';
+					break;
+				case 'E':
+					treemethod = 'E';
+					break;
+				case 'q':
+					treemethod = 'q';
+					break;
+				case 'n' :
+					outnumber = 1;
+					break;
+#if 0
+				case 'a':
+					alg = 'a';
+					break;
+#endif
+				case 'Q':
+					alg = 'Q';
+					break;
+				case 'H':
+					alg = 'H';
+					break;
+				case 'A':
+					alg = 'A';
+					break;
+				case 'M':
+					alg = 'M';
+					break;
+				case 'N':
+					nevermemsave = 1;
+					break;
+				case 'B':
+					break;
+				case 'F':
+					use_fft = 1;
+					break;
+				case 'G':
+					force_fft = 1;
+					use_fft = 1;
+					break;
+				case 'U':
+					treein = 1;
+					break;
+				case 'V':
+					topin = 1;
+					break;
+				case 'u':
+					tbrweight = 0;
+					weight = 0;
+					break;
+				case 'v':
+					tbrweight = 3;
+					break;
+				case 'd':
+					disp = 1;
+					break;
+/* Modified 01/08/27, default: user tree */
+				case 'J':
+					tbutree = 0;
+					break;
+/* modification end. */
+				case 'z':
+					fftThreshold = atoi( *++argv );
+					--argc; 
+					goto nextoption;
+				case 'w':
+					fftWinSize = atoi( *++argv );
+					--argc;
+					goto nextoption;
+				case 'Z':
+					checkC = 1;
+					break;
+                default:
+                    fprintf( stderr, "illegal option %c\n", c );
+                    argc = 0;
+                    break;
+            }
+		}
+		nextoption:
+			;
+	}
+    if( argc == 1 )
+    {
+        cut = atof( (*argv) );
+        argc--;
+    }
+    if( argc != 0 ) 
+    {
+        fprintf( stderr, "options: Check source file !\n" );
+        exit( 1 );
+    }
+	if( tbitr == 1 && outgap == 0 )
+	{
+		fprintf( stderr, "conflicting options : o, m or u\n" );
+		exit( 1 );
+	}
+	if( alg == 'C' && outgap == 0 )
+	{
+		fprintf( stderr, "conflicting options : C, o\n" );
+		exit( 1 );
+	}
+}
+
+#if 0
+static void *distancematrixthread2( void *arg )
+{
+	distancematrixthread_arg_t *targ = (distancematrixthread_arg_t *)arg;
+	int njob = targ->njob;
+	int thread_no = targ->thread_no;
+	float *selfscore = targ->selfscore;
+	float **iscore = targ->iscore;
+	char **seq = targ->seq;
+	Jobtable *jobpospt = targ->jobpospt;
+
+	float ssi, ssj, bunbo;
+	int i, j;
+
+	while( 1 )
+	{
+		pthread_mutex_lock( targ->mutex );
+		i = jobpospt->i;
+		i++;
+		if( i == njob-1 )
+		{
+			pthread_mutex_unlock( targ->mutex );
+			return( NULL );
+		}
+		jobpospt->i = i;
+		pthread_mutex_unlock( targ->mutex );
+
+		ssi = selfscore[i];
+		if( i % 10 == 0 ) fprintf( stderr, "\r% 5d / %d (thread %4d)", i, njob, thread_no );
+		for( j=i+1; j<njob; j++)
+		{
+			ssj = selfscore[j];
+			bunbo = MIN( ssi, ssj );
+			if( bunbo == 0.0 )
+				iscore[i][j-i] = 1.0;
+			else
+				iscore[i][j-i] = 1.0 - naivepairscore11( seq[i], seq[j], penalty ) / bunbo;
+		}
+	}
+}
+#endif
+
+#ifdef enablemultithread
+static void *distancematrixthread( void *arg )
+{
+	distancematrixthread_arg_t *targ = (distancematrixthread_arg_t *)arg;
+	int njob = targ->njob;
+	int thread_no = targ->thread_no;
+	float *selfscore = targ->selfscore;
+	float **iscore = targ->iscore;
+	char **seq = targ->seq;
+	Jobtable *jobpospt = targ->jobpospt;
+
+	float ssi, ssj, bunbo;
+	int i, j;
+
+	while( 1 )
+	{
+		pthread_mutex_lock( targ->mutex );
+		j = jobpospt->j;
+		i = jobpospt->i;
+		j++;
+		if( j == njob )
+		{
+			i++;
+			j = i + 1;
+			if( i == njob-1 )
+			{
+				pthread_mutex_unlock( targ->mutex );
+				return( NULL );
+			}
+		}
+		jobpospt->j = j;
+		jobpospt->i = i;
+		pthread_mutex_unlock( targ->mutex );
+
+
+		if( j==i+1 && i % 10 == 0 ) fprintf( stderr, "\r% 5d / %d (thread %4d)", i, njob, thread_no );
+		ssi = selfscore[i];
+		ssj = selfscore[j];
+		bunbo = MIN( ssi, ssj );
+		if( bunbo == 0.0 )
+			iscore[i][j-i] = 1.0;
+		else
+			iscore[i][j-i] = 1.0 - naivepairscore11( seq[i], seq[j], penalty ) / bunbo;
+	}
+}
+
+static void *treebasethread( void *arg )
+{
+	treebasethread_arg_t *targ = (treebasethread_arg_t *)arg;
+	int *nrunpt = targ->nrunpt;
+	int thread_no = targ->thread_no;
+	int njob = targ->njob;
+	int *nlen = targ->nlen;
+	int *jobpospt = targ->jobpospt;
+	int ***topol = targ->topol;
+	Treedep *dep = targ->dep;
+	char **aseq = targ->aseq;
+	double *effarr = targ->effarr;
+	int *alloclen = targ->alloclenpt;
+	LocalHom **localhomtable = targ->localhomtable;
+	RNApair ***singlerna = targ->singlerna;
+	double *effarr_kozo = targ->effarr_kozo;
+	int *fftlog = targ->fftlog;
+
+	char **mseq1, **mseq2;
+	char **localcopy;
+	int i, j, l;
+	int len1, len2;
+	int clus1, clus2;
+	float pscore;
+	char *indication1, *indication2;
+	double *effarr1 = NULL;
+	double *effarr2 = NULL;
+	double *effarr1_kozo = NULL;
+	double *effarr2_kozo = NULL;
+	LocalHom ***localhomshrink = NULL;
+	int m1, m2;
+	float dumfl = 0.0;
+	int ffttry;
+	RNApair ***grouprna1, ***grouprna2;
+
+
+	mseq1 = AllocateCharMtx( njob, 0 );
+	mseq2 = AllocateCharMtx( njob, 0 );
+	localcopy = calloc( njob, sizeof( char * ) );
+
+	if( rnakozo && rnaprediction == 'm' )
+	{
+		grouprna1 = (RNApair ***)calloc( njob, sizeof( RNApair ** ) );
+		grouprna2 = (RNApair ***)calloc( njob, sizeof( RNApair ** ) );
+	}
+	else
+	{
+		grouprna1 = grouprna2 = NULL;
+	}
+
+	effarr1 = AllocateDoubleVec( njob );
+	effarr2 = AllocateDoubleVec( njob );
+	indication1 = AllocateCharVec( 150 );
+	indication2 = AllocateCharVec( 150 );
+#if 0
+#else
+	if( constraint )
+	{
+		localhomshrink = (LocalHom ***)calloc( njob, sizeof( LocalHom ** ) );
+		for( i=0; i<njob; i++)
+			localhomshrink[i] = (LocalHom **)calloc( njob, sizeof( LocalHom *) );
+	}
+#endif
+	effarr1_kozo = AllocateDoubleVec( njob ); //tsuneni allocate sareru.
+	effarr2_kozo = AllocateDoubleVec( njob ); //tsuneni allocate sareru.
+	for( i=0; i<njob; i++ ) effarr1_kozo[i] = 0.0;
+	for( i=0; i<njob; i++ ) effarr2_kozo[i] = 0.0;
+
+
+#if 0
+#endif
+
+#if 0
+	for( i=0; i<njob; i++ )
+		fprintf( stderr, "TBFAST effarr[%d] = %f\n", i, effarr[i] );
+#endif
+
+#if 0 //-> main thread
+	if( constraint )
+		calcimportance( njob, effarr, aseq, localhomtable );
+#endif
+
+
+//	writePre( njob, name, nlen, aseq, 0 );
+
+
+//	for( l=0; l<njob-1; l++ )
+	while( 1 )
+	{
+
+		pthread_mutex_lock( targ->mutex );
+		l = *jobpospt;
+		if( l == njob-1 )
+		{
+			pthread_mutex_unlock( targ->mutex );
+			if( commonIP ) FreeIntMtx( commonIP );
+			commonIP = NULL;
+			Falign( NULL, NULL, NULL, NULL, 0, 0, 0, NULL, NULL, 0, NULL );
+			A__align( NULL, NULL, NULL, NULL, 0, 0, 0, NULL, NULL, NULL, NULL, NULL, NULL, NULL, 0, NULL, 0, 0 );
+			free( mseq1 );
+			free( mseq2 );
+			free( localcopy );
+			free( effarr1 );
+			free( effarr2 );
+			free( effarr1_kozo );
+			free( effarr2_kozo );
+			free( indication1 );
+			free( indication2 );
+			if( constraint )
+			{
+				for( i=0; i<njob; i++)
+					free( localhomshrink[i] );
+				free( localhomshrink );
+			}
+			return( NULL );
+		}
+		*jobpospt = l+1;
+
+		if( dep[l].child0 != -1 )
+		{
+			while( dep[dep[l].child0].done == 0 )
+				pthread_cond_wait( targ->treecond, targ->mutex );
+		}
+		if( dep[l].child1 != -1 )
+		{
+			while( dep[dep[l].child1].done == 0 )
+				pthread_cond_wait( targ->treecond, targ->mutex );
+		}
+//		while( *nrunpt >= nthread )
+//			pthread_cond_wait( targ->treecond, targ->mutex );
+		(*nrunpt)++;
+
+//		pthread_mutex_unlock( targ->mutex );
+
+
+		m1 = topol[l][0][0];
+		m2 = topol[l][1][0];
+
+//		pthread_mutex_lock( targ->mutex );
+
+        len1 = strlen( aseq[m1] );
+        len2 = strlen( aseq[m2] );
+        if( *alloclen <= len1 + len2 )
+        {
+			fprintf( stderr, "\nReallocating (by thread %d) ..", thread_no );
+			*alloclen = ( len1 + len2 ) + 1000;
+			ReallocateCharMtx( aseq, njob, *alloclen + 10  );
+			fprintf( stderr, "done. *alloclen = %d\n", *alloclen );
+		}
+
+		for( i=0; (j=topol[l][0][i])!=-1; i++ )
+		{
+			localcopy[j] = calloc( *alloclen, sizeof( char ) );
+			strcpy( localcopy[j], aseq[j] );
+		}
+		for( i=0; (j=topol[l][1][i])!=-1; i++ )
+		{
+			localcopy[j] = calloc( *alloclen, sizeof( char ) );
+			strcpy( localcopy[j], aseq[j] );
+		}
+
+		pthread_mutex_unlock( targ->mutex );
+
+		if( effarr_kozo )
+		{
+			clus1 = fastconjuction_noname_kozo( topol[l][0], localcopy, mseq1, effarr1, effarr, effarr1_kozo, effarr_kozo, indication1 );
+			clus2 = fastconjuction_noname_kozo( topol[l][1], localcopy, mseq2, effarr2, effarr, effarr2_kozo, effarr_kozo, indication2 );
+		}
+		else
+		{
+			clus1 = fastconjuction_noname( topol[l][0], localcopy, mseq1, effarr1, effarr, indication1 );
+			clus2 = fastconjuction_noname( topol[l][1], localcopy, mseq2, effarr2, effarr, indication2 );
+		}
+
+
+
+#if 1
+		fprintf( stderr, "\rSTEP % 5d /%d (thread %4d) ", l+1, njob-1, thread_no );
+#else
+		fprintf( stderr, "STEP %d /%d (thread %d) \n", l+1, njob-1, thread_no );
+		fprintf( stderr, "group1 = %.66s", indication1 );
+		if( strlen( indication1 ) > 66 ) fprintf( stderr, "..." );
+		fprintf( stderr, ", child1 = %d\n", dep[l].child0 );
+		fprintf( stderr, "group2 = %.66s", indication2 );
+		if( strlen( indication2 ) > 66 ) fprintf( stderr, "..." );
+		fprintf( stderr, ", child2 = %d\n", dep[l].child1 );
+
+		fprintf( stderr, "Group1's lengths = " );
+		for( i=0; i<clus1; i++ ) fprintf( stderr, "%d ", strlen( mseq1[i] ) );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "Group2's lengths = " );
+		for( i=0; i<clus2; i++ ) fprintf( stderr, "%d ", strlen( mseq2[i] ) );
+		fprintf( stderr, "\n" );
+		
+#endif
+
+
+
+//		for( i=0; i<clus1; i++ ) fprintf( stderr, "## STEP%d-eff for mseq1-%d %f\n", l+1, i, effarr1[i] );
+
+		if( constraint )
+		{
+			fastshrinklocalhom( topol[l][0], topol[l][1], localhomtable, localhomshrink );
+//			msfastshrinklocalhom( topol[l][0], topol[l][1], localhomtable, localhomshrink );
+//			fprintf( stderr, "localhomshrink =\n" );
+//			outlocalhompt( localhomshrink, clus1, clus2 );
+//			weightimportance4( clus1, clus2, effarr1, effarr2, localhomshrink );
+//			fprintf( stderr, "after weight =\n" );
+//			outlocalhompt( localhomshrink, clus1, clus2 );
+		}
+		if( rnakozo && rnaprediction == 'm' )
+		{
+			makegrouprna( grouprna1, singlerna, topol[l][0] );
+			makegrouprna( grouprna2, singlerna, topol[l][1] );
+		}
+
+
+/*
+		fprintf( stderr, "before align all\n" );
+		display( localcopy, njob );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "before align 1 %s \n", indication1 );
+		display( mseq1, clus1 );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "before align 2 %s \n", indication2 );
+		display( mseq2, clus2 );
+		fprintf( stderr, "\n" );
+*/
+
+		if( !nevermemsave && ( constraint != 2  && alg != 'M'  && ( len1 > 30000 || len2 > 30000 ) ) )
+		{
+			fprintf( stderr, "\nlen1=%d, len2=%d, Switching to the memsave mode.\n", len1, len2 );
+			alg = 'M';
+			if( commonIP ) FreeIntMtx( commonIP );
+			commonAlloc1 = 0;
+			commonAlloc2 = 0;
+		}
+		
+
+//		if( fftlog[m1] && fftlog[m2] ) ffttry = ( nlen[m1] > clus1 && nlen[m2] > clus2 );
+		if( fftlog[m1] && fftlog[m2] ) ffttry = ( nlen[m1] > clus1 && nlen[m2] > clus2 && clus1 < 1000 && clus2 < 1000 );
+		else						   ffttry = 0;
+//		ffttry = ( nlen[m1] > clus1 && nlen[m2] > clus2 && clus1 < 5000 && clus2 < 5000 ); // v6.708
+//		fprintf( stderr, "f=%d, len1/fftlog[m1]=%f, clus1=%d, len2/fftlog[m2]=%f, clus2=%d\n", ffttry, (float)len1/fftlog[m1], clus1, (float)len2/fftlog[m2], clus2 );
+//		fprintf( stderr, "f=%d, clus1=%d, fftlog[m1]=%d, clus2=%d, fftlog[m2]=%d\n", ffttry, clus1, fftlog[m1], clus2, fftlog[m2] );
+		if( constraint == 2 )
+		{
+			if( alg == 'M' )
+			{
+				fprintf( stderr, "\n\nMemory saving mode is not supported.\n\n" );
+				exit( 1 );
+			}
+			fprintf( stderr, "c" );
+			if( alg == 'A' )
+			{
+				imp_match_init_strict( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, effarr1_kozo, effarr2_kozo, localhomshrink, 1 );
+				if( rnakozo ) imp_rna( clus1, clus2, mseq1, mseq2, effarr1, effarr2, grouprna1, grouprna2, NULL, NULL, NULL );
+				pscore = A__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL, NULL, 0, NULL, outgap, outgap );
+			}
+			else if( alg == 'H' )
+			{
+				imp_match_init_strictH( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, localhomshrink, 1 );
+				pscore = H__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL );
+			}
+			else if( alg == 'Q' )
+			{
+				imp_match_init_strictQ( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, localhomshrink, 1 );
+				if( rnakozo ) imp_rnaQ( clus1, clus2, mseq1, mseq2, effarr1, effarr2, grouprna1, grouprna2, NULL, NULL, NULL );
+				pscore = Q__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL );
+			}
+			else if( alg == 'R' )
+			{
+				imp_match_init_strictR( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, localhomshrink, 1 );
+				pscore = R__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL );
+			}
+		}
+		else if( force_fft || ( use_fft && ffttry ) )
+		{
+			fprintf( stderr, "f" );
+			if( alg == 'M' )
+			{
+				fprintf( stderr, "m" );
+				pscore = Falign_udpari_long( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, fftlog+m1 );
+			}
+			else
+				pscore = Falign( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, fftlog+m1, NULL, 0, NULL );
+		}
+		else
+		{
+			fprintf( stderr, "d" );
+			fftlog[m1] = 0;
+			switch( alg )
+			{
+				case( 'a' ):
+					pscore = Aalign( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen );
+					break;
+				case( 'M' ):
+					fprintf( stderr, "m" );
+					pscore = MSalignmm( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, NULL, NULL, NULL, NULL, 0, NULL, outgap, outgap );
+					break;
+				case( 'A' ):
+					pscore = A__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL, NULL, 0, NULL, outgap, outgap );
+					break;
+				case( 'Q' ):
+					pscore = Q__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL );
+					break;
+				case( 'R' ):
+					pscore = R__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL );
+					break;
+				case( 'H' ):
+					pscore = H__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL );
+					break;
+				default:
+					ErrorExit( "ERROR IN SOURCE FILE" );
+			}
+		}
+
+		nlen[m1] = 0.5 * ( nlen[m1] + nlen[m2] );
+
+#if SCOREOUT
+		fprintf( stderr, "score = %10.2f\n", pscore );
+#endif
+
+/*
+		fprintf( stderr, "after align 1 %s \n", indication1 );
+		display( mseq1, clus1 );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "after align 2 %s \n", indication2 );
+		display( mseq2, clus2 );
+		fprintf( stderr, "\n" );
+*/
+
+//		writePre( njob, name, nlen, localcopy, 0 );
+
+		if( disp ) display( localcopy, njob );
+
+		pthread_mutex_lock( targ->mutex );
+		dep[l].done = 1;
+		(*nrunpt)--;
+		pthread_cond_broadcast( targ->treecond );
+
+//		pthread_mutex_unlock( targ->mutex );
+//		pthread_mutex_lock( targ->mutex );
+
+		for( i=0; (j=topol[l][0][i])!=-1; i++ )
+			strcpy( aseq[j], localcopy[j] );
+		for( i=0; (j=topol[l][1][i])!=-1; i++ )
+			strcpy( aseq[j], localcopy[j] );
+		pthread_mutex_unlock( targ->mutex );
+
+		for( i=0; (j=topol[l][0][i])!=-1; i++ )
+			free( localcopy[j] );
+		for( i=0; (j=topol[l][1][i])!=-1; i++ )
+			free( localcopy[j] );
+		free( topol[l][0] );
+		free( topol[l][1] );
+		free( topol[l] );
+
+	}
+}
+#endif
+
+void treebase( int *nlen, char **aseq, int nadd, char *mergeoralign, char **mseq1, char **mseq2, int ***topol, double *effarr, int *alloclen, LocalHom **localhomtable, RNApair ***singlerna, double *effarr_kozo )
+{
+	int i, l, m;
+	int len1nocommongap, len2nocommongap;
+	int len1, len2;
+	int clus1, clus2;
+	float pscore, tscore;
+	static char *indication1, *indication2;
+	static double *effarr1 = NULL;
+	static double *effarr2 = NULL;
+	static double *effarr1_kozo = NULL;
+	static double *effarr2_kozo = NULL;
+	static LocalHom ***localhomshrink = NULL;
+	static int *fftlog;
+	int m1, m2;
+	static int *gaplen;
+	static int *gapmap;
+	static int *alreadyaligned;
+	float dumfl = 0.0;
+	int ffttry;
+	RNApair ***grouprna1, ***grouprna2;
+
+	if( rnakozo && rnaprediction == 'm' )
+	{
+		grouprna1 = (RNApair ***)calloc( njob, sizeof( RNApair ** ) );
+		grouprna2 = (RNApair ***)calloc( njob, sizeof( RNApair ** ) );
+	}
+	else
+	{
+		grouprna1 = grouprna2 = NULL;
+	}
+
+	if( effarr1 == NULL ) 
+	{
+		fftlog = AllocateIntVec( njob );
+		effarr1 = AllocateDoubleVec( njob );
+		effarr2 = AllocateDoubleVec( njob );
+		indication1 = AllocateCharVec( 150 );
+		indication2 = AllocateCharVec( 150 );
+		gaplen = AllocateIntVec( *alloclen+10 );
+		gapmap = AllocateIntVec( *alloclen+10 );
+		alreadyaligned = AllocateIntVec( njob );
+#if 0
+#else
+		if( constraint )
+		{
+			localhomshrink = (LocalHom ***)calloc( njob, sizeof( LocalHom ** ) );
+			for( i=0; i<njob; i++)
+				localhomshrink[i] = (LocalHom **)calloc( njob, sizeof( LocalHom *) );
+		}
+#endif
+		effarr1_kozo = AllocateDoubleVec( njob ); //tsuneni allocate sareru.
+		effarr2_kozo = AllocateDoubleVec( njob ); //tsuneni allocate sareru.
+		for( i=0; i<njob; i++ ) effarr1_kozo[i] = 0.0;
+		for( i=0; i<njob; i++ ) effarr2_kozo[i] = 0.0;
+	}
+
+	for( i=0; i<njob-nadd; i++ ) alreadyaligned[i] = 1;
+	for( i=njob-nadd; i<njob; i++ ) alreadyaligned[i] = 0;
+
+	for( l=0; l<njob; l++ ) fftlog[l] = 1;
+
+#if 0
+	fprintf( stderr, "##### fftwinsize = %d, fftthreshold = %d\n", fftWinSize, fftThreshold );
+#endif
+
+#if 0
+	for( i=0; i<njob; i++ )
+		fprintf( stderr, "TBFAST effarr[%d] = %f\n", i, effarr[i] );
+#endif
+
+
+	if( constraint )
+		calcimportance( njob, effarr, aseq, localhomtable );
+
+
+//	writePre( njob, name, nlen, aseq, 0 );
+
+	tscore = 0.0;
+	for( l=0; l<njob-1; l++ )
+	{
+		if( mergeoralign[l] == 'n' )
+		{
+//			fprintf( stderr, "SKIP!\n" );
+			free( topol[l][0] );
+			free( topol[l][1] );
+			free( topol[l] );
+			continue;
+		}
+
+		m1 = topol[l][0][0];
+		m2 = topol[l][1][0];
+        len1 = strlen( aseq[m1] );
+        len2 = strlen( aseq[m2] );
+        if( *alloclen < len1 + len2 )
+        {
+			fprintf( stderr, "\nReallocating.." );
+			*alloclen = ( len1 + len2 ) + 1000;
+			ReallocateCharMtx( aseq, njob, *alloclen + 10  );
+			gaplen = realloc( gaplen, ( *alloclen + 10 ) * sizeof( int ) );
+			if( gaplen == NULL )
+			{
+				fprintf( stderr, "Cannot realloc gaplen\n" );
+				exit( 1 );
+			}
+			gapmap = realloc( gapmap, ( *alloclen + 10 ) * sizeof( int ) );
+			if( gapmap == NULL )
+			{
+				fprintf( stderr, "Cannot realloc gapmap\n" );
+				exit( 1 );
+			}
+			fprintf( stderr, "done. *alloclen = %d\n", *alloclen );
+		}
+
+		if( effarr_kozo )
+		{
+			clus1 = fastconjuction_noname_kozo( topol[l][0], aseq, mseq1, effarr1, effarr, effarr1_kozo, effarr_kozo, indication1 );
+			clus2 = fastconjuction_noname_kozo( topol[l][1], aseq, mseq2, effarr2, effarr, effarr2_kozo, effarr_kozo, indication2 );
+		}
+		else
+		{
+			clus1 = fastconjuction_noname( topol[l][0], aseq, mseq1, effarr1, effarr, indication1 );
+			clus2 = fastconjuction_noname( topol[l][1], aseq, mseq2, effarr2, effarr, indication2 );
+		}
+
+		if( mergeoralign[l] == '1' || mergeoralign[l] == '2' )
+		{
+			newgapstr = "=";
+		}
+		else
+			newgapstr = "-";
+
+
+		len1nocommongap = len1;
+		len2nocommongap = len2;
+		if( mergeoralign[l] == '1' ) // nai
+		{
+			findcommongaps( clus2, mseq2, gapmap );
+			commongappick( clus2, mseq2 );
+			len2nocommongap = strlen( mseq2[0] );
+		}
+		else if( mergeoralign[l] == '2' )
+		{
+			findcommongaps( clus1, mseq1, gapmap );
+			commongappick( clus1, mseq1 );
+			len1nocommongap = strlen( mseq1[0] );
+		}
+		
+
+		fprintf( trap_g, "\nSTEP-%d\n", l );
+		fprintf( trap_g, "group1 = %s\n", indication1 );
+		fprintf( trap_g, "group2 = %s\n", indication2 );
+
+#if 1
+		fprintf( stderr, "\rSTEP % 5d /%d ", l+1, njob-1 );
+		fflush( stderr );
+#else
+		fprintf( stdout, "STEP %d /%d\n", l+1, njob-1 );
+		fprintf( stderr, "STEP %d /%d\n", l+1, njob-1 );
+		fprintf( stderr, "group1 = %.66s", indication1 );
+		if( strlen( indication1 ) > 66 ) fprintf( stderr, "..." );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "group2 = %.66s", indication2 );
+		if( strlen( indication2 ) > 66 ) fprintf( stderr, "..." );
+		fprintf( stderr, "\n" );
+#endif
+
+
+
+//		for( i=0; i<clus1; i++ ) fprintf( stderr, "## STEP%d-eff for mseq1-%d %f\n", l+1, i, effarr1[i] );
+
+		if( constraint )
+		{
+			fastshrinklocalhom( topol[l][0], topol[l][1], localhomtable, localhomshrink );
+//			msfastshrinklocalhom( topol[l][0], topol[l][1], localhomtable, localhomshrink );
+//			fprintf( stderr, "localhomshrink =\n" );
+//			outlocalhompt( localhomshrink, clus1, clus2 );
+//			weightimportance4( clus1, clus2, effarr1, effarr2, localhomshrink );
+//			fprintf( stderr, "after weight =\n" );
+//			outlocalhompt( localhomshrink, clus1, clus2 );
+		}
+		if( rnakozo && rnaprediction == 'm' )
+		{
+			makegrouprna( grouprna1, singlerna, topol[l][0] );
+			makegrouprna( grouprna2, singlerna, topol[l][1] );
+		}
+
+
+/*
+		fprintf( stderr, "before align all\n" );
+		display( aseq, njob );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "before align 1 %s \n", indication1 );
+		display( mseq1, clus1 );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "before align 2 %s \n", indication2 );
+		display( mseq2, clus2 );
+		fprintf( stderr, "\n" );
+*/
+
+		if( !nevermemsave && ( constraint != 2  && alg != 'M'  && ( len1 > 30000 || len2 > 30000 ) ) )
+		{
+			fprintf( stderr, "\nlen1=%d, len2=%d, Switching to the memsave mode.\n", len1, len2 );
+			alg = 'M';
+			if( commonIP ) FreeIntMtx( commonIP );
+			commonAlloc1 = 0;
+			commonAlloc2 = 0;
+		}
+		
+
+//		if( fftlog[m1] && fftlog[m2] ) ffttry = ( nlen[m1] > clus1 && nlen[m2] > clus2 );
+		if( fftlog[m1] && fftlog[m2] ) ffttry = ( nlen[m1] > clus1 && nlen[m2] > clus2 && clus1 < 1000 && clus2 < 1000 );
+		else						   ffttry = 0;
+//		ffttry = ( nlen[m1] > clus1 && nlen[m2] > clus2 && clus1 < 5000 && clus2 < 5000 ); // v6.708
+//		fprintf( stderr, "f=%d, len1/fftlog[m1]=%f, clus1=%d, len2/fftlog[m2]=%f, clus2=%d\n", ffttry, (float)len1/fftlog[m1], clus1, (float)len2/fftlog[m2], clus2 );
+//		fprintf( stderr, "f=%d, clus1=%d, fftlog[m1]=%d, clus2=%d, fftlog[m2]=%d\n", ffttry, clus1, fftlog[m1], clus2, fftlog[m2] );
+		if( constraint == 2 )
+		{
+			if( alg == 'M' )
+			{
+				fprintf( stderr, "\n\nMemory saving mode is not supported.\n\n" );
+				exit( 1 );
+			}
+			fprintf( stderr, "c" );
+			if( alg == 'A' )
+			{
+				imp_match_init_strict( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, effarr1_kozo, effarr2_kozo, localhomshrink, 1 );
+				if( rnakozo ) imp_rna( clus1, clus2, mseq1, mseq2, effarr1, effarr2, grouprna1, grouprna2, NULL, NULL, NULL );
+				pscore = A__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL, NULL, 0, NULL, outgap, outgap );
+			}
+			else if( alg == 'H' )
+			{
+				imp_match_init_strictH( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, localhomshrink, 1 );
+				pscore = H__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL );
+			}
+			else if( alg == 'Q' )
+			{
+				imp_match_init_strictQ( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, localhomshrink, 1 );
+				if( rnakozo ) imp_rnaQ( clus1, clus2, mseq1, mseq2, effarr1, effarr2, grouprna1, grouprna2, NULL, NULL, NULL );
+				pscore = Q__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL );
+			}
+			else if( alg == 'R' )
+			{
+				imp_match_init_strictR( NULL, clus1, clus2, strlen( mseq1[0] ), strlen( mseq2[0] ), mseq1, mseq2, effarr1, effarr2, localhomshrink, 1 );
+				pscore = R__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, localhomshrink, &dumfl, NULL, NULL, NULL, NULL );
+			}
+		}
+		else if( force_fft || ( use_fft && ffttry ) )
+		{
+			fprintf( stderr, "f" );
+			if( alg == 'M' )
+			{
+				fprintf( stderr, "m" );
+				pscore = Falign_udpari_long( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, fftlog+m1 );
+			}
+			else
+				pscore = Falign( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, fftlog+m1, NULL, 0, NULL );
+		}
+		else
+		{
+			fprintf( stderr, "d" );
+			fftlog[m1] = 0;
+			switch( alg )
+			{
+				case( 'a' ):
+					pscore = Aalign( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen );
+					break;
+				case( 'M' ):
+					fprintf( stderr, "m" );
+					pscore = MSalignmm( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, NULL, NULL, NULL, NULL, 0, NULL, outgap, outgap );
+					break;
+				case( 'A' ):
+					pscore = A__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL, NULL, 0, NULL, outgap, outgap );
+					break;
+				case( 'Q' ):
+					pscore = Q__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL );
+					break;
+				case( 'R' ):
+					pscore = R__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL );
+					break;
+				case( 'H' ):
+					pscore = H__align( mseq1, mseq2, effarr1, effarr2, clus1, clus2, *alloclen, NULL, &dumfl, NULL, NULL, NULL, NULL );
+					break;
+				default:
+					ErrorExit( "ERROR IN SOURCE FILE" );
+			}
+		}
+
+		nlen[m1] = 0.5 * ( nlen[m1] + nlen[m2] );
+
+#if SCOREOUT
+		fprintf( stderr, "score = %10.2f\n", pscore );
+#endif
+		tscore += pscore;
+/*
+		fprintf( stderr, "after align 1 %s \n", indication1 );
+		display( mseq1, clus1 );
+		fprintf( stderr, "\n" );
+		fprintf( stderr, "after align 2 %s \n", indication2 );
+		display( mseq2, clus2 );
+		fprintf( stderr, "\n" );
+*/
+
+//		writePre( njob, name, nlen, aseq, 0 );
+
+		if( disp ) display( aseq, njob );
+
+		if( mergeoralign[l] == '1' ) // jissainiha nai. atarashii hairetsu ha saigo dakara.
+		{
+			adjustgapmap( strlen( mseq2[0] )-len2nocommongap+len2, gapmap, mseq2[0] );
+			restorecommongaps( njob, aseq, topol[l][0], topol[l][1], gapmap, *alloclen );
+			findnewgaps( clus2, mseq2, gaplen );
+			insertnewgaps( njob, alreadyaligned, aseq, topol[l][1], topol[l][0], gaplen, gapmap, *alloclen, alg );
+			for( i=0; i<njob; i++ ) eq2dash( aseq[i] );
+			for( i=0; (m=topol[l][0][i])>-1; i++ ) alreadyaligned[m] = 1;
+		}
+		if( mergeoralign[l] == '2' )
+		{
+//			fprintf( stderr, ">mseq1[0] = \n%s\n", mseq1[0] );
+//			fprintf( stderr, ">mseq2[0] = \n%s\n", mseq2[0] );
+			adjustgapmap( strlen( mseq1[0] )-len1nocommongap+len1, gapmap, mseq1[0] );
+			restorecommongaps( njob, aseq, topol[l][0], topol[l][1], gapmap, *alloclen );
+			findnewgaps( clus1, mseq1, gaplen );
+			insertnewgaps( njob, alreadyaligned, aseq, topol[l][0], topol[l][1], gaplen, gapmap, *alloclen, alg );
+			for( i=0; i<njob; i++ ) eq2dash( aseq[i] );
+			for( i=0; (m=topol[l][1][i])>-1; i++ ) alreadyaligned[m] = 1;
+		}
+
+		free( topol[l][0] );
+		free( topol[l][1] );
+		free( topol[l] );
+	}
+#if SCOREOUT
+	fprintf( stderr, "totalscore = %10.2f\n\n", tscore );
+#endif
+}
+
+static void WriteOptions( FILE *fp )
+{
+
+	if( dorp == 'd' ) fprintf( fp, "DNA\n" );
+	else
+	{
+		if     ( scoremtx ==  0 ) fprintf( fp, "JTT %dPAM\n", pamN );
+		else if( scoremtx ==  1 ) fprintf( fp, "BLOSUM %d\n", nblosum );
+		else if( scoremtx ==  2 ) fprintf( fp, "M-Y\n" );
+	}
+    fprintf( stderr, "Gap Penalty = %+5.2f, %+5.2f, %+5.2f\n", (double)ppenalty/1000, (double)ppenalty_ex/1000, (double)poffset/1000 );
+    if( use_fft ) fprintf( fp, "FFT on\n" );
+
+	fprintf( fp, "tree-base method\n" );
+	if( tbrweight == 0 ) fprintf( fp, "unweighted\n" );
+	else if( tbrweight == 3 ) fprintf( fp, "clustalw-like weighting\n" );
+	if( tbitr || tbweight ) 
+	{
+		fprintf( fp, "iterate at each step\n" );
+		if( tbitr && tbrweight == 0 ) fprintf( fp, "  unweighted\n" ); 
+		if( tbitr && tbrweight == 3 ) fprintf( fp, "  reversely weighted\n" ); 
+		if( tbweight ) fprintf( fp, "  weighted\n" ); 
+		fprintf( fp, "\n" );
+	}
+
+   	 fprintf( fp, "Gap Penalty = %+5.2f, %+5.2f, %+5.2f\n", (double)ppenalty/1000, (double)ppenalty_ex/1000, (double)poffset/1000 );
+
+	if( alg == 'a' )
+		fprintf( fp, "Algorithm A\n" );
+	else if( alg == 'A' ) 
+		fprintf( fp, "Algorithm A+\n" );
+	else if( alg == 'C' ) 
+		fprintf( fp, "Apgorithm A+/C\n" );
+	else
+		fprintf( fp, "Unknown algorithm\n" );
+
+	if( treemethod == 'X' )
+		fprintf( fp, "Tree = UPGMA (mix).\n" );
+	else if( treemethod == 'E' )
+		fprintf( fp, "Tree = UPGMA (average).\n" );
+	else if( treemethod == 'q' )
+		fprintf( fp, "Tree = Minimum linkage.\n" );
+	else
+		fprintf( fp, "Unknown tree.\n" );
+
+    if( use_fft )
+    {
+        fprintf( fp, "FFT on\n" );
+        if( dorp == 'd' )
+            fprintf( fp, "Basis : 4 nucleotides\n" );
+        else
+        {
+            if( fftscore )
+                fprintf( fp, "Basis : Polarity and Volume\n" );
+            else
+                fprintf( fp, "Basis : 20 amino acids\n" );
+        }
+        fprintf( fp, "Threshold   of anchors = %d%%\n", fftThreshold );
+        fprintf( fp, "window size of anchors = %dsites\n", fftWinSize );
+    }
+	else
+        fprintf( fp, "FFT off\n" );
+	fflush( fp );
+}
+	 
+
+int main( int argc, char *argv[] )
+{
+	static int  *nlen;	
+	static float *selfscore;
+	int nogaplen;
+	static char **name, **seq;
+	static char **mseq1, **mseq2;
+	static char **bseq;
+	static float **iscore, **iscore_kozo;
+	static double *eff, *eff_kozo, *eff_kozo_mapped = NULL;
+	int i, j, ien, ik, jk;
+	static int ***topol, ***topol_kozo;
+	static int *addmem;
+	static Treedep *dep;
+	static float **len, **len_kozo;
+	FILE *prep;
+	FILE *infp;
+	FILE *orderfp;
+	FILE *hat2p;
+	double unweightedspscore;
+	int alignmentlength;
+	char *mergeoralign;
+	int foundthebranch;
+	
+	char c;
+	int alloclen;
+	LocalHom **localhomtable = NULL;
+	RNApair ***singlerna;
+	float ssi, ssj, bunbo;
+	static char *kozoarivec;
+	int nkozo;
+
+	arguments( argc, argv );
+#ifndef enablemultithread
+	nthread = 0;
+#endif
+
+	if( inputfile )
+	{
+		infp = fopen( inputfile, "r" );
+		if( !infp ) 
+		{
+			fprintf( stderr, "Cannot open %s\n", inputfile );
+			exit( 1 );
+		}
+	}
+	else    
+		infp = stdin;
+
+	getnumlen( infp );
+	rewind( infp );
+
+
+	nkozo = 0;
+
+	if( njob < 2 )
+	{
+		fprintf( stderr, "At least 2 sequences should be input!\n"
+						 "Only %d sequence found.\n", njob ); 
+		exit( 1 );
+	}
+
+	seq = AllocateCharMtx( njob, nlenmax+1 );
+	mseq1 = AllocateCharMtx( njob, 0 );
+	mseq2 = AllocateCharMtx( njob, 0 );
+
+	name = AllocateCharMtx( njob, B+1 );
+	nlen = AllocateIntVec( njob );
+	selfscore = AllocateFloatVec( njob );
+
+	topol = AllocateIntCub( njob, 2, 0 );
+	len = AllocateFloatMtx( njob, 2 );
+	iscore = AllocateFloatHalfMtx( njob );
+	eff = AllocateDoubleVec( njob );
+	kozoarivec = AllocateCharVec( njob );
+
+	mergeoralign = AllocateCharVec( njob );
+
+	dep = (Treedep *)calloc( njob, sizeof( Treedep ) );
+	if( nadd ) addmem = AllocateIntVec( nadd+1 );
+
+	if( constraint )
+	{
+		localhomtable = (LocalHom **)calloc( njob, sizeof( LocalHom *) );
+		for( i=0; i<njob; i++)
+		{
+			localhomtable[i] = (LocalHom *)calloc( njob, sizeof( LocalHom ) );
+			for( j=0; j<njob; j++)
+			{
+				localhomtable[i][j].start1 = -1;
+				localhomtable[i][j].end1 = -1;
+				localhomtable[i][j].start2 = -1;
+				localhomtable[i][j].end2 = -1;
+				localhomtable[i][j].overlapaa = -1.0;
+				localhomtable[i][j].opt = -1.0;
+				localhomtable[i][j].importance = -1.0;
+				localhomtable[i][j].next = NULL;
+				localhomtable[i][j].korh = 'h';
+			}
+		}
+
+		fprintf( stderr, "Loading 'hat3' ... " );
+		prep = fopen( "hat3", "r" );
+		if( prep == NULL ) ErrorExit( "Make hat3." );
+		readlocalhomtable( prep, njob, localhomtable, kozoarivec );
+		fclose( prep );
+		fprintf( stderr, "\ndone.\n" );
+
+
+		nkozo = 0;
+		for( i=0; i<njob; i++ ) 
+		{
+//			fprintf( stderr, "kozoarivec[%d] = %d\n", i, kozoarivec[i] );
+			if( kozoarivec[i] ) nkozo++;
+		}
+		if( nkozo )
+		{
+			topol_kozo = AllocateIntCub( nkozo, 2, 0 );
+			len_kozo = AllocateFloatMtx( nkozo, 2 );
+			iscore_kozo = AllocateFloatHalfMtx( nkozo );
+			eff_kozo = AllocateDoubleVec( nkozo );
+			eff_kozo_mapped = AllocateDoubleVec( njob );
+		}
+
+
+//		outlocalhom( localhomtable, njob );
+
+#if 0
+		fprintf( stderr, "Extending localhom ... " );
+		extendlocalhom2( njob, localhomtable );
+		fprintf( stderr, "done.\n" );
+#endif
+	}
+
+#if 0
+	readData( infp, name, nlen, seq );
+#else
+	readData_pointer( infp, name, nlen, seq );
+	fclose( infp );
+#endif
+
+	constants( njob, seq );
+
+#if 0
+	fprintf( stderr, "params = %d, %d, %d\n", penalty, penalty_ex, offset );
+#endif
+
+	initSignalSM();
+
+	initFiles();
+
+	WriteOptions( trap_g );
+
+	c = seqcheck( seq );
+	if( c )
+	{
+		fprintf( stderr, "Illegal character %c\n", c );
+		exit( 1 );
+	}
+
+//	writePre( njob, name, nlen, seq, 0 );
+
+	if( treein )
+	{
+#if 0
+		if( nkozo )
+		{
+			fprintf( stderr, "Both structure and user tree have been given. Not yet supported!\n" );
+			exit( 1 );
+		}
+#endif
+		fprintf( stderr, "Loading a tree ... " );
+		loadtree( njob, topol, len, name, nlen, dep );
+		fprintf( stderr, "\ndone.\n\n" );
+	}
+	else
+	{
+		if( tbutree == 0 )
+		{
+			for( i=1; i<njob; i++ ) 
+			{
+				if( nlen[i] != nlen[0] ) 
+				{
+					fprintf( stderr, "Input pre-aligned seqences or make hat2.\n" );
+					exit( 1 );
+				}
+			}
+	
+			fprintf( stderr, "Making a distance matrix .. \n" );
+			fflush( stderr );
+			ien = njob-1;
+			for( i=0; i<njob; i++ ) 
+			{
+				selfscore[i] = naivepairscore11( seq[i], seq[i], penalty );
+			}
+#ifdef enablemultithread
+			if( nthread > 0 )
+			{
+				distancematrixthread_arg_t *targ;
+				Jobtable jobpos;
+				pthread_t *handle;
+				pthread_mutex_t mutex;
+
+				jobpos.i = 0;
+				jobpos.j = 0;
+
+				targ = calloc( nthread, sizeof( distancematrixthread_arg_t ) );
+				handle = calloc( nthread, sizeof( pthread_t ) );
+				pthread_mutex_init( &mutex, NULL );
+
+				for( i=0; i<nthread; i++ )
+				{
+					targ[i].thread_no = i;
+					targ[i].njob = njob;
+					targ[i].selfscore = selfscore;
+					targ[i].iscore = iscore;
+					targ[i].seq = seq;
+					targ[i].jobpospt = &jobpos;
+					targ[i].mutex = &mutex;
+
+					pthread_create( handle+i, NULL, distancematrixthread, (void *)(targ+i) );
+				}
+
+				for( i=0; i<nthread; i++ )
+				{
+					pthread_join( handle[i], NULL );
+				}
+				pthread_mutex_destroy( &mutex );
+				free( handle );
+				free( targ );
+			}
+			else
+#endif
+			{
+				for( i=0; i<ien; i++ ) 
+				{
+					if( i % 10 == 0 )
+					{
+						fprintf( stderr, "\r% 5d / %d", i, ien );
+						fflush( stderr );
+					}
+					ssi = selfscore[i];
+					for( j=i+1; j<njob; j++ ) 
+					{
+						ssj = selfscore[j];
+						bunbo = MIN( ssi, ssj );
+						if( bunbo == 0.0 )
+							iscore[i][j-i] = 1.0;
+						else
+//							iscore[i][j-i] = 1.0 - naivepairscore11( seq[i], seq[j], penalty ) / MIN( selfscore[i], selfscore[j] );
+							iscore[i][j-i] = 1.0 - naivepairscore11( seq[i], seq[j], penalty ) / bunbo;
+		
+#if 0
+						fprintf( stderr, "### ssj = %f\n", ssj );
+						fprintf( stderr, "### selfscore[i] = %f\n", selfscore[i] );
+						fprintf( stderr, "### selfscore[j] = %f\n", selfscore[j] );
+						fprintf( stderr, "### rawscore = %f\n", naivepairscore11( seq[i], seq[j], penalty ) );
+#endif
+					}
+				}
+			}
+			fprintf( stderr, "\ndone.\n\n" );
+			fflush( stderr );
+		}
+		else
+		{
+			fprintf( stderr, "Loading 'hat2' ... " );
+			prep = fopen( "hat2", "r" );
+			if( prep == NULL ) ErrorExit( "Make hat2." );
+			readhat2_floathalf_pointer( prep, njob, name, iscore );
+			fclose( prep );
+			fprintf( stderr, "done.\n" );
+		}
+#if 1
+		if( distout )
+		{
+			hat2p = fopen( "hat2", "w" );
+			WriteFloatHat2_pointer_halfmtx( hat2p, njob, name, iscore );
+			fclose( hat2p );
+		}
+#endif
+		if( nkozo )
+		{
+			ien = njob-1;
+			ik = 0;
+			for( i=0; i<ien; i++ )
+			{
+				jk = ik+1;
+				for( j=i+1; j<njob; j++ ) 
+				{
+					if( kozoarivec[i] && kozoarivec[j] )
+					{
+						iscore_kozo[ik][jk-ik] = iscore[i][j-i];
+					}
+					if( kozoarivec[j] ) jk++;
+				}
+				if( kozoarivec[i] ) ik++;
+			}
+		}
+
+		fprintf( stderr, "Constructing a UPGMA tree ... " );
+		fflush( stderr );
+		if( topin )
+		{
+			fprintf( stderr, "Loading a topology ... " );
+			loadtop( njob, iscore, topol, len );
+			fprintf( stderr, "\ndone.\n\n" );
+		}
+		else if( treeout )
+		{
+			fixed_musclesupg_float_realloc_nobk_halfmtx_treeout( njob, iscore, topol, len, name, nlen, dep );
+		}
+		else
+		{
+			fixed_musclesupg_float_realloc_nobk_halfmtx( njob, iscore, topol, len, dep );
+		}
+//		else 
+//			ErrorExit( "Incorrect tree\n" );
+
+		if( nkozo )
+		{
+//			for( i=0; i<nkozo-1; i++ )
+//				for( j=i+1; j<nkozo; j++ )
+//					fprintf( stderr, "iscore_kozo[%d][%d] =~ %f\n", i, j, iscore_kozo[i][j-i] );
+			fixed_musclesupg_float_realloc_nobk_halfmtx( nkozo, iscore_kozo, topol_kozo, len_kozo, NULL );
+		}
+		fprintf( stderr, "\ndone.\n\n" );
+		fflush( stderr );
+	}
+
+
+	orderfp = fopen( "order", "w" );
+	if( !orderfp )
+	{
+		fprintf( stderr, "Cannot open 'order'\n" );
+		exit( 1 );
+	}
+	for( i=0; (j=topol[njob-2][0][i])!=-1; i++ )
+	{
+		fprintf( orderfp, "%d\n", j );
+	}
+	for( i=0; (j=topol[njob-2][1][i])!=-1; i++ )
+	{
+		fprintf( orderfp, "%d\n", j );
+	}
+	fclose( orderfp );
+
+	if( treeout && noalign ) 
+	{
+		writeData_pointer( prep_g, njob, name, nlen, seq );
+		fprintf( stderr, "\n" ); 
+		SHOWVERSION;
+		return( 0 );
+	}
+
+//	countnode( njob, topol, node0 );
+	if( tbrweight )
+	{
+		weight = 3; 
+#if 0
+		utree = 0; counteff( njob, topol, len, eff ); utree = 1;
+#else
+		counteff_simple_float( njob, topol, len, eff );
+
+		if( nkozo )
+		{
+//			counteff_simple_float( nkozo, topol_kozo, len_kozo, eff_kozo ); // single weight nanode iranai
+			for( i=0,j=0; i<njob; i++ )
+			{
+				if( kozoarivec[i] )
+				{
+//					eff_kozo_mapped[i] = eff_kozo[j]; //
+					eff_kozo_mapped[i] = eff[i];      // single weight
+					j++;
+				}
+				else
+					eff_kozo_mapped[i] = 0.0;
+//				fprintf( stderr, "eff_kozo_mapped[%d] = %f\n", i, eff_kozo_mapped[i] );
+//				fprintf( stderr, "            eff[%d] = %f\n", i, eff[i] );
+			}
+		}
+
+
+#endif
+	}
+	else
+	{
+		for( i=0; i<njob; i++ ) eff[i] = 1.0;
+		if( nkozo ) 
+		{
+			for( i=0; i<njob; i++ ) 
+			{
+				if( kozoarivec[i] ) 
+					eff_kozo_mapped[i] = 1.0;
+				else
+					eff_kozo_mapped[i] = 0.0;
+			}
+		}
+	}
+
+	FreeFloatHalfMtx( iscore, njob );
+	FreeFloatMtx( len );
+
+	alloclen = nlenmax*2+1; //chuui!
+	bseq = AllocateCharMtx( njob, alloclen );
+
+	if( nadd )
+	{
+		alignmentlength = strlen( seq[0] );
+		for( i=0; i<njob-nadd; i++ )
+		{
+			if( alignmentlength != strlen( seq[i] ) )
+			{
+				fprintf( stderr, "#################################################################################\n" );
+				fprintf( stderr, "# ERROR!                                                                        #\n" );
+				fprintf( stderr, "# The original%4d sequences must be aligned                                    #\n", njob-nadd );
+				fprintf( stderr, "#################################################################################\n" );
+				exit( 1 );
+			}
+		}
+		if( addprofile )
+		{
+			alignmentlength = strlen( seq[njob-nadd] );
+			for( i=njob-nadd; i<njob; i++ )
+			{
+				if( alignmentlength != strlen( seq[i] ) )
+				{
+					fprintf( stderr, "###############################################################################\n" );
+					fprintf( stderr, "# ERROR!                                                                      #\n" );
+					fprintf( stderr, "# The%4d additional sequences must be aligned                                #\n", nadd );
+					fprintf( stderr, "# Otherwise, try the '--add' option, instead of '--addprofile' option.        #\n" );
+					fprintf( stderr, "###############################################################################\n" );
+					exit( 1 );
+				}
+			}
+			for( i=0; i<nadd; i++ ) addmem[i] = njob-nadd+i;
+			addmem[nadd] = -1;
+			foundthebranch = 0;
+			for( i=0; i<njob-1; i++ )
+			{
+				if( samemember( topol[i][0], addmem ) ) // jissainiha nai
+				{
+					mergeoralign[i] = '1';
+					foundthebranch = 1;
+				}
+				else if( samemember( topol[i][1], addmem ) )
+				{
+					mergeoralign[i] = '2';
+					foundthebranch = 1;
+				}
+				else
+				{
+					mergeoralign[i] = 'n';
+				}
+			}
+			if( !foundthebranch )
+			{
+				fprintf( stderr, "###############################################################################\n" );
+				fprintf( stderr, "# ERROR!                                                                      #\n" );
+				fprintf( stderr, "# There is no appropriate position to add the%4d sequences in the guide tree.#\n", nadd );
+				fprintf( stderr, "# Check whether the%4d sequences form a monophyletic cluster.                #\n", nadd );
+				fprintf( stderr, "# If not, try the '--add' option, instead of the '--addprofile' option.       #\n" );
+				fprintf( stderr, "############################################################################### \n" );
+				exit( 1 );
+			}
+			commongappick( nadd, seq+njob-nadd );
+			for( i=njob-nadd; i<njob; i++ ) strcpy( bseq[i], seq[i] );
+		}
+		else
+		{
+			for( i=0; i<njob-1; i++ ) mergeoralign[i] = 'n';
+			for( j=njob-nadd; j<njob; j++ )
+			{
+				addmem[0] = j;
+				addmem[1] = -1;
+				for( i=0; i<njob-1; i++ )
+				{
+					if( samemember( topol[i][0], addmem ) ) // arieru
+					{
+//						fprintf( stderr, "HIT!\n" );
+						if( mergeoralign[i] != 'n' ) mergeoralign[i] = 'w';
+						else mergeoralign[i] = '1';
+					}
+					else if( samemember( topol[i][1], addmem ) )
+					{
+//						fprintf( stderr, "HIT!\n" );
+						if( mergeoralign[i] != 'n' ) mergeoralign[i] = 'w';
+						else mergeoralign[i] = '2';
+					}
+				}
+			}
+	
+			for( i=0; i<nadd; i++ ) addmem[i] = njob-nadd+i;
+			addmem[nadd] = -1;
+			for( i=0; i<njob-1; i++ )
+			{
+				if( includemember( topol[i][0], addmem ) && includemember( topol[i][1], addmem ) )
+				{
+					mergeoralign[i] = 'w';
+				}
+				else if( includemember( topol[i][0], addmem ) )
+				{
+					mergeoralign[i] = '1';
+				}
+				else if( includemember( topol[i][1], addmem ) )
+				{
+					mergeoralign[i] = '2';
+				}
+			}
+#if 0
+			for( i=0; i<njob-1; i++ )
+			{
+				fprintf( stderr, "mem0 = " );
+				for( j=0; topol[i][0][j]>-1; j++ )	fprintf( stderr, "%d ", topol[i][0][j] );
+				fprintf( stderr, "\n" );
+				fprintf( stderr, "mem1 = " );
+				for( j=0; topol[i][1][j]>-1; j++ )	fprintf( stderr, "%d ", topol[i][1][j] );
+				fprintf( stderr, "\n" );
+				fprintf( stderr, "i=%d, mergeoralign[] = %c\n", i, mergeoralign[i] );
+			}
+#endif
+			for( i=njob-nadd; i<njob; i++ ) gappick0( bseq[i], seq[i] );
+		}
+
+		commongappick( njob-nadd, seq );
+		for( i=0; i<njob-nadd; i++ ) strcpy( bseq[i], seq[i] );
+	}
+	else
+	{
+		for( i=0; i<njob; i++ ) gappick0( bseq[i], seq[i] );
+		for( i=0; i<njob-1; i++ ) mergeoralign[i] = 'a';
+	}
+
+	if( rnakozo && rnaprediction == 'm' )
+	{
+		singlerna = (RNApair ***)calloc( njob, sizeof( RNApair ** ) );
+		prep = fopen( "hat4", "r" );
+		if( prep == NULL ) ErrorExit( "Make hat4 using mccaskill." );
+		fprintf( stderr, "Loading 'hat4' ... " );
+		for( i=0; i<njob; i++ )
+		{
+			nogaplen = strlen( bseq[i] );
+			singlerna[i] = (RNApair **)calloc( nogaplen, sizeof( RNApair * ) );
+			for( j=0; j<nogaplen; j++ )
+			{
+				singlerna[i][j] = (RNApair *)calloc( 1, sizeof( RNApair ) );
+				singlerna[i][j][0].bestpos = -1;
+				singlerna[i][j][0].bestscore = -1.0;
+			}
+			readmccaskill( prep, singlerna[i], nogaplen );
+		}
+		fclose( prep );
+		fprintf( stderr, "\ndone.\n" );
+	}
+	else
+		singlerna = NULL;
+
+
+	fprintf( stderr, "Progressive alignment ... \n" );
+
+#ifdef enablemultithread
+	if( nthread > 0 && nadd == 0 )
+	{
+		treebasethread_arg_t *targ;	
+		int jobpos;
+		pthread_t *handle;
+		pthread_mutex_t mutex;
+		pthread_cond_t treecond;
+		int *fftlog;
+		int nrun;
+		int nthread_yoyu;
+
+		nthread_yoyu = nthread * 1;
+		nrun = 0;
+		jobpos = 0;
+		targ = calloc( nthread_yoyu, sizeof( treebasethread_arg_t ) );
+		fftlog = AllocateIntVec( njob );
+		handle = calloc( nthread_yoyu, sizeof( pthread_t ) );
+		pthread_mutex_init( &mutex, NULL );
+		pthread_cond_init( &treecond, NULL );
+
+		for( i=0; i<njob; i++ ) dep[i].done = 0;
+		for( i=0; i<njob; i++ ) fftlog[i] = 1;
+
+		if( constraint )
+			calcimportance( njob, eff, bseq, localhomtable );
+
+		for( i=0; i<nthread_yoyu; i++ )
+		{
+			targ[i].thread_no = i;
+			targ[i].nrunpt = &nrun;
+			targ[i].njob = njob;
+			targ[i].nlen = nlen;
+			targ[i].jobpospt = &jobpos;
+			targ[i].topol = topol;
+			targ[i].dep = dep;
+			targ[i].aseq = bseq;
+			targ[i].effarr = eff;
+			targ[i].alloclenpt = &alloclen;
+			targ[i].localhomtable = localhomtable;
+			targ[i].singlerna = singlerna;
+			targ[i].effarr_kozo = eff_kozo_mapped;
+			targ[i].fftlog = fftlog;
+			targ[i].mutex = &mutex;
+			targ[i].treecond = &treecond;
+
+			pthread_create( handle+i, NULL, treebasethread, (void *)(targ+i) );
+		}
+
+		for( i=0; i<nthread_yoyu; i++ )
+		{
+			pthread_join( handle[i], NULL );
+		}
+		pthread_mutex_destroy( &mutex );
+		pthread_cond_destroy( &treecond );
+		free( handle );
+		free( targ );
+		free( fftlog );
+	}
+	else
+#endif
+		treebase( nlen, bseq, nadd, mergeoralign, mseq1, mseq2, topol, eff, &alloclen, localhomtable, singlerna, eff_kozo_mapped );
+	fprintf( stderr, "\ndone.\n" );
+	if( scoreout )
+	{
+		unweightedspscore = plainscore( njob, bseq );
+		fprintf( stderr, "\nSCORE %s = %.0f, ", "(treebase)", unweightedspscore );
+		fprintf( stderr, "SCORE / residue = %f", unweightedspscore / ( njob * strlen( bseq[0] ) ) );
+		fprintf( stderr, "\n\n" );
+	}
+
+#if 0
+	if( constraint )
+	{
+		LocalHom *tmppt1, *tmppt2;
+		for( i=0; i<njob; i++)
+		{
+			for( j=0; j<njob; j++)
+			{
+				tmppt1 = localhomtable[i]+j;
+				while( tmppt2 = tmppt1->next )
+				{
+					free( (void *)tmppt1 );
+					tmppt1 = tmppt2;
+				}
+				free( (void *)tmppt1 );
+			}
+			free( (void *)(localhomtable[i]+j) );
+		}
+		free( (void *)localhomtable );
+	}
+#endif
+
+	fprintf( trap_g, "done.\n" );
+	fclose( trap_g );
+	free( mergeoralign );
+
+	writeData_pointer( prep_g, njob, name, nlen, bseq );
+#if 0
+	writeData( stdout, njob, name, nlen, bseq );
+	writePre( njob, name, nlen, bseq, !contin );
+	writeData_pointer( prep_g, njob, name, nlen, aseq );
+#endif
+#if IODEBUG
+	fprintf( stderr, "OSHIMAI\n" );
+#endif
+
+	if( constraint ) FreeLocalHomTable( localhomtable, njob );
+
+	SHOWVERSION;
+	return( 0 );
+}