X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=forester%2Fruby%2Fevoruby%2Fexe%2Frun_phylo_pipeline.rb;h=5cd0401fd3857b67ecc1759c3455049236e3d049;hb=d49bf58cee106ada674e7fa0d7fcf328ed797252;hp=a5f8304003e0b4b056e716f6c08c41d7bfea5da5;hpb=9d4be44622758e8cb1c971075da9663ae7dbe59c;p=jalview.git

diff --git a/forester/ruby/evoruby/exe/run_phylo_pipeline.rb b/forester/ruby/evoruby/exe/run_phylo_pipeline.rb
index a5f8304..5cd0401 100755
--- a/forester/ruby/evoruby/exe/run_phylo_pipeline.rb
+++ b/forester/ruby/evoruby/exe/run_phylo_pipeline.rb
@@ -14,45 +14,46 @@ module Evoruby
 
   class RunPhyloPipeline
 
-    PFAM      = "/home/czmasek/DATA/PFAM/PFAM260X/"
+    PFAM      = "/home/czmasek/DATA/PFAM/PFAM270X/"
     HMMSCAN  = "/home/czmasek/SOFTWARE/HMMER/hmmer-3.0/src/hmmscan"
     HSP       = "/home/czmasek/SOFTWARE/FORESTER/DEV/forester/forester/ruby/evoruby/exe/hsp.rb"
     D2F       = "/home/czmasek/SOFTWARE/FORESTER/DEV/forester/forester/ruby/evoruby/exe/d2f.rb"
     DSX       = "/home/czmasek/SOFTWARE/FORESTER/DEV/forester/forester/ruby/evoruby/exe/dsx.rb"
 
-
     def run
-      unless ARGV.length == 4
-        error "arguments are: <fasta formatted inputfile> <hmm-name> <min-length> <neg e-value exponent>"
+      unless ARGV.length >= 4 && ARGV.length <= 6
+        error "arguments are: <fasta formatted inputfile> <hmm-name> <min-length> " +
+         "<neg E-value exponent for domain extraction> [E-value for hmmscan, default is 20] [hmmscan option, default is --nobias, --max for no heuristics]"
       end
 
-
-
-
       input       = ARGV[ 0 ]
       hmm         = ARGV[ 1 ]
       length      = ARGV[ 2 ].to_i
       e_value_exp = ARGV[ 3 ].to_i
 
+      e_for_hmmscan = 20
       hmmscan_option = "--nobias"
-      e_for_hmmscan = 100
+
+      if ARGV.length == 6
+        hmmscan_option = ARGV[ 5 ]
+      end
+      if ARGV.length == 5 || ARGV.length == 6
+        e_for_hmmscan = ARGV[ 4 ].to_i
+      end
 
       if e_value_exp < 0
-        error "e-value exponent cannot be negative"
+        error "E-value exponent for domain extraction cannot be negative"
       end
       if length <= 1
-        error "length exponent cannot be smaller than or equal to 1"
+        error "length cannot be smaller than or equal to 1"
       end
-
-      base_name = nil
-      if input.downcase.end_with?( ".fasta" )
-        base_name = input[ 0 .. input.length - 7 ]
-      elsif input.downcase.end_with?( ".fsa" )
-        base_name = input[ 0 .. input.length - 5 ]
-      else
-        base_name = input
+      if e_for_hmmscan < 1
+        error "E-value for hmmscan cannot be smaller than 1"
       end
 
+      base_name = get_base_name input
+
+      puts
       puts "1. hmmscan:"
       cmd = "#{HMMSCAN} #{hmmscan_option} --domtblout #{base_name}_hmmscan_#{e_for_hmmscan.to_s} -E #{e_for_hmmscan.to_s} #{PFAM}Pfam-A.hmm #{input}"
       run_command( cmd )
@@ -69,17 +70,31 @@ module Evoruby
       puts
 
       puts "4. dsx:"
-      cmd = "#{DSX} -d -e=1e-#{e_value_exp.to_s} -l=#{length} #{hmm} #{base_name}.hmmscan_#{e_for_hmmscan.to_s} #{input} #{base_name}_#{hmm}_e#{e_value_exp.to_s}_#{length}"
+      cmd = "#{DSX} -d -e=1e-#{e_value_exp.to_s} -l=#{length} #{hmm} #{base_name}_hmmscan_#{e_for_hmmscan.to_s} #{input} #{base_name}__#{hmm}__ee#{e_value_exp.to_s}_#{length}"
       run_command( cmd )
       puts
 
     end
 
-    def run_command( cmd )
+    def run_command cmd
       puts cmd
       `#{cmd}`
     end
 
+    def get_base_name n
+      if n.downcase.end_with?( "_ni.fasta" )
+        n[ 0 .. n.length - 10 ]
+      elsif n.downcase.end_with?( ".fasta" )
+        n[ 0 .. n.length - 7 ]
+      elsif n.downcase.end_with?( "_ni.fsa" )
+        n[ 0 .. n.length - 8 ]
+      elsif n.downcase.end_with?( ".fsa" )
+        n[ 0 .. n.length - 5 ]
+      else
+        n
+      end
+    end
+
     def error msg
       puts
       puts msg