X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=forester%2Fruby%2Fevoruby%2Fexe%2Fselect_same_gn.rb;h=7dbe9f10017de23937b82744ba2295233f776497;hb=b35ac7de74589875baabdd2f7b5fe8d5cd43ab33;hp=275a1492364a52e47b78e69981abc1fabbcdb2cd;hpb=6f3385fbe81811e9daea8cfe02dce9b1cf61c9dc;p=jalview.git

diff --git a/forester/ruby/evoruby/exe/select_same_gn.rb b/forester/ruby/evoruby/exe/select_same_gn.rb
index 275a149..7dbe9f1 100755
--- a/forester/ruby/evoruby/exe/select_same_gn.rb
+++ b/forester/ruby/evoruby/exe/select_same_gn.rb
@@ -10,9 +10,18 @@ require 'lib/evo/io/parser/fasta_parser'
 
 module Evoruby
 
+  if ARGV.length != 1
+    puts "usage: select_same_gn.rb <fasta formatted multiple sequence file>"
+    exit
+  end
+
   input = ARGV[ 0 ]
   f = MsaFactory.new()
 
+  IGNORE_SEQS_LACKING_GN = false
+  IGNORE_FRAGMENTS = false
+  IGNORE_SPECIES = true
+
   msa = nil
 
   begin
@@ -22,48 +31,115 @@ module Evoruby
     exit
   end
 
+  outbase = input.sub( /\..+/, "" )
+
+  outfile = File.open(outbase + "_log.txt" , "w")
+
+  all_names = Set.new
+  all_seqs_per_species = Hash.new
+  all_msa_per_species = Hash.new
   gn_to_seqs = Hash.new
   unique_genes_msa = Msa.new
   longest_non_unique_genes_msa = Msa.new
   gn_re = /GN=(\S+)/
   fragment_re = /fragment/i
+  species_re = /\[([A-Z0-9]{3,5})\]$/
 
   frag_counter = 0
   no_gn_counter = 0
+  same_seq_counter = 0
 
   for i in 0 ... msa.get_number_of_seqs()
     seq = msa.get_sequence( i )
     name = seq.get_name
-    if fragment_re.match( name )
-      puts "ignored because fragment: " + name
+    if all_names.include?( name )
+      puts "error: sequence name \"" + name + "\" is not unique (#" + i.to_s + ")"
+      exit
+    else
+      all_names << name
+    end
+
+    if IGNORE_FRAGMENTS && fragment_re.match( name )
+      outfile.puts("ignored because fragment: " + name)
       frag_counter += 1
       next
     end
+
+    species = nil
+    if IGNORE_SPECIES || species_re.match( name )
+      unless IGNORE_SPECIES
+        species = species_re.match( name )[ 1 ]
+      else
+        species = "XXXXX"
+      end
+
+      unless all_seqs_per_species.has_key?( species )
+        all_seqs_per_species[ species ] = Set.new
+      end
+      all_seqs = all_seqs_per_species[ species ]
+      mol_seq = seq.get_sequence_as_string.upcase
+      if all_seqs.include?( mol_seq )
+        outfile.puts("ignored because identical sequence in same species: " + name )
+
+        same_seq_counter += 1
+        next
+      else
+        all_seqs << mol_seq
+      end
+    else
+      puts "error: no species for: " + name
+      exit
+    end
+
     gn_match = gn_re.match( name )
-    unless gn_match
-      puts "ignored because no GN=: " + name
-      no_gn_counter += 1
-      next
+    if IGNORE_SEQS_LACKING_GN
+      unless gn_match
+        outfile.puts( "ignored because no GN=: " + name )
+        no_gn_counter += 1
+        next
+      end
+    else
+      unless gn_match
+        outfile.puts( "no GN=: " + name )
+      end
+    end
+
+    gn =nil
+    if gn_match
+      gn = gn_match[1] + "_" + species
+    else
+      if IGNORE_SEQS_LACKING_GN
+        puts "cannot be"
+        exit
+      end
+      gn = name
     end
-    gn = gn_match[1]
+
     unless gn_to_seqs.has_key?(gn)
       gn_to_seqs[gn] = Msa.new
     end
     gn_to_seqs[gn].add_sequence(seq)
   end
 
-  puts "Sequeunces ignored because \"fragment\" in desc: " + frag_counter.to_s
-  puts "Sequeunces ignored because no \"GN=\" in desc  : " + no_gn_counter.to_s
-  puts
-  puts
+  if IGNORE_FRAGMENTS
+    outfile.puts( "Sequences ignored because \"fragment\" in desc                : " + frag_counter.to_s )
+  end
+  
+  if IGNORE_SEQS_LACKING_GN
+    outfile.puts( "Sequences ignored because no \"GN=\" in desc                  : " + no_gn_counter.to_s )
+  end
+  outfile.puts( "Sequences ignored because identical sequence in same species: " + same_seq_counter.to_s )
+  outfile.puts
+  outfile.puts
 
   counter = 1
   gn_to_seqs.each_pair do |gene,seqs|
+    seq = nil
     if seqs.get_number_of_seqs > 1
-      puts counter.to_s + ": " + gene
-      puts seqs.to_fasta
-      puts
-      puts
+      outfile.puts( counter.to_s + ": " + gene )
+      outfile.puts( seqs.to_fasta )
+      outfile.puts
+      outfile.puts
       counter += 1
       longest = 0
       longest_seq = nil
@@ -74,12 +150,35 @@ module Evoruby
           longest_seq = current
         end
       end
-      longest_non_unique_genes_msa.add_sequence(longest_seq)
+      seq = longest_seq
+      longest_non_unique_genes_msa.add_sequence( seq )
+    else
+      seq = seqs.get_sequence( 0 )
+      unique_genes_msa.add_sequence( seq )
+    end
+
+
+    unless IGNORE_SPECIES
+      species = species_re.match( seq.get_name )[ 1 ]
     else
-      unique_genes_msa.add_sequence( seqs.get_sequence( 0 ) )
+      species = "XXXXX"
     end
+    unless all_msa_per_species.has_key?( species )
+      all_msa_per_species[ species ] = Msa.new
+    end
+    all_msa_per_species[ species ].add_sequence( seq )
+
   end
+
+  outfile.close
+
   w = FastaWriter.new
-  w.write(unique_genes_msa, "seqs_from_unique_genes.fasta")
-  w.write(longest_non_unique_genes_msa, "longest_seqs_from_nonunique_genes.fasta")
+  w.write( unique_genes_msa, outbase + "_seqs_from_unique_genes.fasta" )
+  w.write( longest_non_unique_genes_msa, outbase + "_longest_seqs_from_nonunique_genes.fasta" )
+
+  all_msa_per_species.each_pair do |s,m|
+    w = FastaWriter.new
+    w.write( m, outbase + "_"  + s + ".fasta" )
+  end
+
 end