Sequence Features File
-The Sequence features file (which used to be known as the -"Groups file" prior to version 2.08) is a simple way of -getting your own sequence annotations into Jalview. It was introduced to -allow sequence features to be rendered in the Jalview applet, and so is -intentionally lightweight and minimal because the applet is often used -in situations where data file size must be kept to a minimum, and no XML -parser is available.
-Features files are imported into Jalview in the following ways:
-
- -features <Features filename>
Sequence Features File Format
-A features file is a simple ASCII text file, where each line -contains tab separated text fields. No comments are -allowed.
-The first set of lines contain type definitions:
-Feature label Feature Colour
A feature
-type has a text label, and a colour (specified as a red,green,blue 24
-bit triplet either in hexadecimal (eg. 00ff00) or as comma separated
-numbers (ranging from 0 to 255)).
The remaining lines in the file are the sequence annotation -definitions, where the now defined features are attached to regions on -particular sequences, optionally with some descriptive text (displayed -in a tooltip when the mouse is near the feature on that sequence). There -are two alternate ways of referring to a sequence, either by its text -ID, or its index in an associated alignment. -
-description sequenceId sequenceIndex start end featureType-Normally, sequence features are associated with sequences rather than -alignments, and the sequenceIndex field is given as "-1". In -order to specify a sequence by its index in a particular alignment, the -sequenceId should be given as "ID_NOT_SPECIFIED", otherwise -the sequenceId field will be used in preference to the sequenceIndex -field. - -
The description may contain simple HTML
-document body tags if enclosed by "<html></html>" and
-these will be rendered as formatted tooltips in the Jalview Application
-(the Jalview applet is not capable of rendering HTML tooltips, so all
-formatting tags will be removed).
-Attaching Links to Sequence Features
-
Any anchor tags in an html formatted description line will be translated
-into URL links. A link symbol will be displayed adjacent to any feature which
-includes links, and these are made available from the
-links submenu of the
-popup menu which is obtained by right-clicking when a link symbol is
-displayed in the tooltip.
-Non-positional features
-Specify the start and end for a feature to be 0 in order to attach it to the whole sequence.
-Non-positional features are shown in a tooltip when the mouse hovers over the seuqence ID panel, and any embedded links can be accessed from the popup menu.
-
Feature annotations can be collected into named groups by
-prefixing definitions with lines of the form:startgroup groupname
..
-and subsequently post-fixing the group with:endgroup groupname
Feature
-grouping was introduced in version 2.08, and used to control whether a
-set of features are either hidden or shown together in the sequence Feature settings dialog box.
A complete example is shown below : -
++ Sequence Features File +
+
The Sequence features file (which used to be known as the + "Groups file" prior to version 2.08) is a simple way of getting your + own sequence annotations into Jalview. It was introduced to allow + sequence features to be rendered in the Jalview applet, and so is + intentionally lightweight and minimal because the applet is often + used in situations where data file size must be kept to a minimum, + and no XML parser is available.
+ ++ Features files are imported into Jalview in the following ways:
+
+ -features <Features filename> ++
+ Sequence Features File Format +
++ A features file is a simple ASCII text file, where each line + contains tab separated text fields. No comments are + allowed. +
+The first set of lines contain type definitions: +
+Feature label Feature Colour + ++ + A feature type has a text label, and a colour specification. This can + be either: + +
+[label|]<mincolor>|<maxcolor>|[absolute|]<minvalue>|<maxvalue>[|<thresholdtype>|[<threshold value>]] +The fields are as follows: + +
The remaining lines in the file are the sequence annotation + definitions, where the now defined features are attached to regions + on particular sequences. Each feature can optionally include some + descriptive text which is displayed in a tooltip when the mouse is + near the feature on that sequence (and can also be used to generate + a colour the feature).
+ ++ If your sequence annotation is already available in GFF Format (see + http://www.sanger.ac.uk/resources/software/gff/spec.html), + then you can leave it as is, after first adding a line containing + only 'GFF' after any Jalview feature colour definitions (this + mixed format capability was added in Jalview 2.6). Alternately, + you can use Jalview's own sequence feature annotation format, which + additionally allows HTML and URLs to be directly attached to each + piece of annotation. +
+ ++ Jalview's sequence feature annotation format +
+Each feature is specified as a tab-separated series of columns + as defined below: +
+description sequenceId sequenceIndex start end featureType score (optional) ++ + This format allows two alternate ways of referring to a sequence, + either by its text ID, or its index in an associated alignment. + Normally, sequence features are associated with sequences rather than + alignments, and the sequenceIndex field is given as "-1". In + order to specify a sequence by its index in a particular alignment, + the sequenceId should be given as "ID_NOT_SPECIFIED", + otherwise the sequenceId field will be used in preference to the + sequenceIndex field. + + + +
+ The description may contain simple HTML document body tags if
+ enclosed by "<html></html>" and these will be
+ rendered as formatted tooltips in the Jalview Application (the
+ Jalview applet is not capable of rendering HTML tooltips, so all
+ formatting tags will be removed).
Attaching Links
+ to Sequence Features
Any anchor tags in an html formatted
+ description line will be translated into URL links. A link symbol
+ will be displayed adjacent to any feature which includes links, and
+ these are made available from the links submenu of the popup menu which is obtained by
+ right-clicking when a link symbol is displayed in the tooltip.
+ Non-positional features
Specify the start
+ and end for a feature to be 0 in order to
+ attach it to the whole sequence. Non-positional features are shown
+ in a tooltip when the mouse hovers over the sequence ID panel, and
+ any embedded links can be accessed from the popup menu.
Scores
+ Scores can be associated with sequence features, and used to sort
+ sequences or shade the alignment (this was added in Jalview 2.5).
+ The score field is optional, and malformed scores will be ignored.
+
Feature annotations can be collected into named groups by + prefixing definitions with lines of the form: +
+startgroup groupname ++ + .. and subsequently post-fixing the group with: + +
+endgroup groupname ++ + Feature grouping was introduced in version 2.08, and used to control + whether a set of features are either hidden or shown together in the + sequence Feature settings dialog box. + + + +
A complete example is shown below : +
domain red metal ion-binding site 00ff00 transit peptide 0,105,215 @@ -91,8 +214,9 @@ startgroup secondarystucture PDB secondary structure annotation FER1_SPIOL -1 52 59 strand PDB secondary structure annotation FER1_SPIOL -1 74 80 helix endgroup secondarystructure +GFF +FER_CAPAA GffGroup domain 3 93 . .- - +