Sequence Features File Format
-(Prior to version 2.08 known as the "Groups file")
- A precalculated Features file can read onto an alignment from the command line
- ("-features"), by drag and dropping the features file onto an alignment
- or by selecting from the File menu "Load Features / Annotations".
Specify the feature types first, then refer to the feature type for each sequence.
-featureType<tab>colour
- description<tab>sequenceId<tab>sequenceIndex<tab>start<tab>end<tab>featureType
eg
- domain red
- metal ion-binding site 00ff00
- transit peptide 0,105,215
- chain 225,105,0
- modified residue 105,225,35
- signal peptide 0,155,165
- Your Own description here FER_CAPAA -1 3 93 domain
- Your Own description here FER_CAPAN -1 48 144 chain
- Your Own description here FER_CAPAN -1 50 140 domain
- Your Own description here FER_CAPAN -1 136 136 modified residue
- Your Own description here FER1_LYCES -1 1 47 transit peptide
- Your Own description here Q93XJ9_SOLTU -1 1 48 signal peptide
- Your Own description here Q93XJ9_SOLTU -1 49 144 chain
An additional option in Jalview 2.08 is to group features in the following - way:
-STARTGROUP<tab>My feature groupA
- ....Many Feature descriptions here
- ENDGROUP<tab>My feature groupA
-
Sequence Features File
+
+The Sequence features file (which used to be known as the +"Groups file" prior to version 2.08) is a simple way of getting +your own sequence annotations into Jalview. It was introduced to +allow sequence features to be rendered in the Jalview applet, and +so is intentionally lightweight and minimal because the applet is +often used in situations where data file size must be kept to a +minimum, and no XML parser is available.
+ +Features files are imported into Jalview in the following
+ways:
+
+
+ -features <Features filename> ++
Sequence Features File Format
++A features file is a simple ASCII text file, where each line +contains tab separated text fields. No comments are +allowed.
++The first set of lines contain type definitions: + +
+Feature label Feature Colour + ++ +A feature type has a text label, and a colour specification. This +can be either: + +
+[label|]<mincolor>|<maxcolor>|[absolute|]<minvalue>|<maxvalue>[|<thresholdtype>|[<threshold value>]] ++ +The fields are as follows: + +
The remaining lines in the file are the sequence annotation +definitions, where the now defined features are attached to regions +on particular sequences. Each feature can optionally include some descriptive text +which is displayed in a tooltip when the mouse is near the feature on that +sequence (and can also be used to generate a colour the feature).
+ +If your sequence annotation is already available in GFF Format (see http://www.sanger.ac.uk/resources/software/gff/spec.html), +then you can leave it as is, after first adding a line containing +only 'GFF' after any Jalview feature colour definitions (this mixed format capability was added in Jalview 2.6). Alternately, you can use Jalview's own sequence feature +annotation format, which additionally allows HTML and URLs to be +directly attached to each piece of annotation.
+ +Jalview's sequence feature annotation format
++Each feature is specified as a tab-separated series of columns as defined below: +
+description sequenceId sequenceIndex start end featureType score (optional) ++ +This format allows two alternate ways of referring to a sequence, either by +its text ID, or its index in an associated alignment. Normally, sequence features are associated with sequences rather +than alignments, and the sequenceIndex field is given as "-1". In +order to specify a sequence by its index in a particular alignment, +the sequenceId should be given as "ID_NOT_SPECIFIED", otherwise the +sequenceId field will be used in preference to the sequenceIndex +field. + + + +
The description may contain simple HTML document body tags if
+enclosed by "<html></html>" and these will be rendered
+as formatted tooltips in the Jalview Application (the Jalview
+applet is not capable of rendering HTML tooltips, so all formatting
+tags will be removed).
+Attaching Links to Sequence Features
+Any anchor tags in an html formatted description line will be
+translated into URL links. A link symbol will be displayed adjacent
+to any feature which includes links, and these are made available
+from the links
+submenu of the popup menu which is obtained by right-clicking
+when a link symbol is displayed in the tooltip.
+Non-positional features
+Specify the start and end for a feature to be
+0 in order to attach it to the whole sequence.
+Non-positional features are shown in a tooltip when the mouse
+hovers over the sequence ID panel, and any embedded links can be
+accessed from the popup menu. Scores
+Scores can be associated with sequence features, and used to sort
+sequences or shade the alignment (this was added in jalview 2.5).
+The score field is optional, and malformed scores will be
+ignored.
Feature annotations can be collected into named groups by +prefixing definitions with lines of the form: + +
+startgroup groupname ++ +.. and subsequently post-fixing the group with: + +
+endgroup groupname ++ +Feature grouping was introduced in version 2.08, and used to +control whether a set of features are either hidden or shown +together in the sequence Feature +settings dialog box. + + +
A complete example is shown below : +
+domain red +metal ion-binding site 00ff00 +transit peptide 0,105,215 +chain 225,105,0 +modified residue 105,225,35 +signal peptide 0,155,165 +helix ff0000 +strand 00ff00 +coil cccccc +Your Own description here FER_CAPAA -1 3 93 domain +Your Own description here FER_CAPAN -1 48 144 chain +Your Own description here FER_CAPAN -1 50 140 domain +Your Own description here FER_CAPAN -1 136 136 modified residue +Your Own description here FER1_LYCES -1 1 47 transit peptide +Your Own description here Q93XJ9_SOLTU -1 1 48 signal peptide +Your Own description here Q93XJ9_SOLTU -1 49 144 chain +startgroup secondarystucture +PDB secondary structure annotation FER1_SPIOL -1 52 59 strand +PDB secondary structure annotation FER1_SPIOL -1 74 80 helix +endgroup secondarystructure +GFF +FER_CAPAA GffGroup domain 3 93 . . ++ + +