View→Sequence Features
-When this option is selected, sequence features extracted from the Uniprot - record for each sequence are displayed on the alignment.
-Jalview will attempt to retrieve sequence features from Uniprot files using - the EBI dbFetch web service using the given sequence names. A 100% match with - the Uniprot record is required to view the Sequence Features. If the match is - not 100%, Jalview will run EBI web service WUBlast to find unknown sequences. - You will be asked whether to proceed with this step, as it can take some time, - especially if the EBI servers are busy.
-More information about the feature is given in a tooltip, which is - viewed by moving the mouse pointer over a sequence feature. The - description associated with the feature will then be displayed in a small - label near the pointer.
-View→Feature Settings... -
Once sequence features have been loaded onto an alignment features can be hidden
- or have their rendering priority changed using the Feature Settings dialog.
- This displays all the features loaded, the colour and whether to display the
- feature or not. You can easily change the colour by clicking the colour box.
- It is important to realise that the order in which features are drawn to the
- alignment may result in overlapping features being hidden. Features at the foot
- of the table are rendered first. Therefore features higher up the table will
- be drawn over the top of lower featrues. You can change the order of the feature
- priority by dragging the feature with your mouse.
- Use the transparency setting as another way to visualise overlapping features.
-
You can save all features, with their current colours and visibility - in a Jalview format file. -
The Sequence Identification Process - -
-The first step in the procedure for matching uniprot IDs to - sequences is to use the ID (name) of - each sequence to retrieve Uniprot records directly.
-- If a uniprot record (or set of records) is found for a sequence, - then the sequence is aligned to the one in the Uniprot record - to determine the correct start and end residue positions (which are - displayed when the 'Show Full Sequence ID' option is set). -
- -If the alignment reveals differences between the sequence in the - alignment and the one in the record, then Jalview will assume that - the aligned sequence is not the one in the uniprot record. - -
- -- In some cases, the ID used to retrieve Uniprot records may be out of - date and you will be notified of that a 100% match between the - sequence and a Uniprot record was identified, but the sequence name - must be manually changed (by right clicking on the sequence ID and selecting - Sequence→Edit Name), before Jalview will show its sequence - features.
Sequence Features
+Jalview can colour parts of a sequence based on the presence of +sequence features - which may be retrieved from database records (such +as Uniprot), the result of sequence motif +searches or simply read from a sequence +features file. You can also create +features from the results of searches or the current selection, and edit features by double clicking on +them.
+Sequence Feature Colouring Styles
+By default, Jalview will assign a color to each feature based on +its type. These colours can be changed from the feature settings and amend features dialog boxes. Since +Jalview 2.5, it is also possible to define feature +colourschemes to shade features based on their associated scores or text +labels.
+Sequence Feature Groups
+Since Jalview 2.08, sequence features assigned to a sequence can +be organised into groups, which may indicate that the features were all +retrieved from the same database (such as Uniprot features), or +generated by the same analysis process (as might be specified in a sequence features file).
+Sequence Feature Inheritance
+Since Jalview 2.08, sequence features are global to a +set of sequences appearing (independently or together) in many different +alignments. Practically, this means features loaded onto one alignment +can be viewed in any alignments involving the same sequences. The same +sequence appears in different alignments when a new alignment is +generated by submitting an existing set of sequences to one of the +alignment or prediction web services, and when sequences are copied and +pasted into other alignment windows.
+View→Show Sequence Features
+Toggle the display of sequence features in this alignment. If +feature retrieval has not already been carried out, then Jalview will +automatically try to fetch sequence features (as described below).
+View→Sequence Feature Settings...
+Once sequence features have been loaded, their display can be
+fully controlled using the alignment window's Sequence Feature Settings dialog box.
+Feature colour schemes and display parameters are unique to a particular
+alignment, so it is possible to colour the same sequence features
+differently in different alignment views.
+Since Jalview 2.1, it is possible to add DAS
+features to an alignment via the DAS tabbed pane of the feature settings
+window.
View→Sequence ID Tooltip→Show
+Non-Positional features
+Only available in application
+
Toggles the display of non-positional features in the sequence ID +tooltip, and whether they will be included when sequence features are +exported using "File→Export Features".
+Precalculated Sequence Features may be added to an alignment from +the command line, drag and drop, or from the "File→Load +Features / Annotations" menu item. See the Features File Format for more details.
+ +