X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=help%2Fhtml%2Ffeatures%2Fseqfetch.html;h=3eb8178713f13587151cdf9e380abec68d78e3a8;hb=a8f483d04205bb8273ee311c12968b7e86d205fa;hp=283377016d9183abd0b106b5b2e9d24875034697;hpb=b48eb2c8a37a5f7c4e9e361f1ebe7e44e3854cd1;p=jalview.git diff --git a/help/html/features/seqfetch.html b/help/html/features/seqfetch.html index 2833770..3eb8178 100755 --- a/help/html/features/seqfetch.html +++ b/help/html/features/seqfetch.html @@ -1,27 +1,72 @@ - -Sequence Fetcher - -

Sequence Fetcher

-

Jalview can retrieve sequences from certain databases via the -WSDBFetch service provided by the European Bioinformatics Institute.

-

A Sequence Fetcher dialog box can be opened via the "File" - menu on the main desktop in order to retrieve sequences as a new - alignment, or opened via the "File" menu of an existing alignment - to import additional sequences. -

-

Select the database you want to retrieve sequences from, and enter a - the database id (or a semi-colon separated list of several ids) in - the text box. Finally, press OK to initiate the retrieval.

-

- If you are retrieving sequences from the PDB, you can retrieve - specific chains by appending a colon and the chain id to the PDB - id. For example :

 1GAQ:A

-

If you use the sequence fetcher in work for publication, then please cite:

-

Pillai S., Silventoinen V., Kallio K., Senger M., Sobhany S., Tate J., Velankar - S., Golovin A., Henrick K., Rice P., Stoehr P., Lopez R.
- SOAP-based services provided by the European Bioinformatics Institute.
- Nucleic Acids Res. 33(1):W25-W28 (2005)
-
-

- - + + + +Sequence Fetcher + + +

Sequence Fetcher

+

Jalview can retrieve sequences from certain databases using either the +WSDBFetch service provided by the European Bioinformatics Institute, and, since Jalview 2.4, DAS servers capable of the sequence command (configured in DAS settings).

+ The Jalview Sequence Fetcher Dialog Box +

The Sequence Fetcher dialog box can be opened via the "File" + menu on the main desktop in order to retrieve sequences as a new + alignment, or opened via the "File" menu of an existing alignment + to import additional sequences. There may be a short delay when the sequence fetcher is first opened, + whilst Jalview compiles the list of available sequence datasources from the + currently defined DAS server registry. +

+

First, select the database you want to retrieve sequences from + by clicking the button labeled 'Select database retrieval source'. If + a database source is already selected, then the button's label will + change to show the currently selected database.

+ Database selection dialog for fetching sequences (introduced in Jalview 2.8) +

Since Jalview 2.8, the + available databases are shown as a tree in a popup dialog box. The + databases are ordered alphabetically, and if there are many sources + for the same type of sequence identifier, they will be grouped + together in a sub-branch branch labeled with the identifier.

+

Once you have selected the sequence database using the popup dialog box, enter + one or more accession ids (as a semi-colon separated list), or press the + "Example" button to paste the example accession for the currently selected database into the retrieval box. + Finally, press "OK" to initiate the retrieval.

+

Specifying chains for PDB IDs + If you are retrieving sequences from the PDB, you can retrieve + specific chains by appending a colon and the chain id to the PDB + id. For example :

 1GAQ:A
+

+

+ Only retrieving part of a sequence DAS sources + (indicated by a "(DAS)") allow a range to be + specified in addition to a sequence ID. To retrieve 50 residues + starting at position 35 in UNIPROT sequence P73137 using the UNIPROT + DAS server, you would enter "'P73137:35,84'.
Full support for DAS range queries was introduced in Jalview 2.8 +

+ +

If you use the WSDBFetch sequence fetcher services (EMBL, Uniprot, PDB, PFAM, and RFAM) + in work for publication, please cite:

+

Pillai S., Silventoinen V., Kallio K., Senger M., Sobhany S., Tate J., Velankar + S., Golovin A., Henrick K., Rice P., Stoehr P., Lopez R.
+ SOAP-based services provided by the European Bioinformatics Institute.
+ Nucleic Acids Res. 33(1):W25-W28 (2005)
+
+

+ +