X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=help%2Fhtml%2Ffeatures%2Fseqfetch.html;h=5f0744dd994482e8fd5d0858f8f2461204b727d8;hb=b5d61763044c1d72f06ce0e50da2171422a3774b;hp=c4d671aa59d729820cbdc8e3ffed27858c3a80a6;hpb=152aa52bc19a393141cfc19f5852d1fbcd239f16;p=jalview.git diff --git a/help/html/features/seqfetch.html b/help/html/features/seqfetch.html index c4d671a..5f0744d 100755 --- a/help/html/features/seqfetch.html +++ b/help/html/features/seqfetch.html @@ -1,74 +1,74 @@ + * You should have received a copy of the GNU General Public License + * along with Jalview. If not, see . + * The Jalview Authors are detailed in the 'AUTHORS' file. + --> Sequence Fetcher

Sequence Fetcher

-

Jalview can retrieve sequences from certain databases using -either the WSDBFetch service provided by the European Bioinformatics -Institute, and, since Jalview 2.4, DAS servers capable of the sequence -command (configured in DAS settings).

-The Jalview Sequence Fetcher Dialog Box -

The Sequence Fetcher dialog box can be opened via the -"File" menu on the main desktop in order to retrieve sequences -as a new alignment, or opened via the "File" menu of an -existing alignment to import additional sequences. Please note, there -will be a short delay when the sequence fetcher is first opened, whilst -Jalview compiles the list of available sequence datasources from the -currently defined DAS server registry.

-

First, select the database you want to retrieve sequences from. -Then, enter one or more accession ids (as a semi-colon separated list), -or press the "Example" button to paste the example accession -for the currently selected database into the retrieval box. Finally, -press "OK" to initiate the retrieval.

-

Fetching Individual PDB Chains
-If you are retrieving sequences from the PDB, you can retrieve specific -chains by appending a colon and the chain id to the PDB id. For example -:
-

 1GAQ:A
-
-When retrieving from DAS sequence sources, coordinate range arguments -can be passed to the server using the standard DAS sequence command -format ( -:<start>,<end> -) -

-

Retrieving parts of large sequence records
-Some sequence sources allow a range query to be appended to the sequence -ID - e.g. 'Mito:1,85779'. If you know a source understands this type of -query format, then you should untick the checkbox for 'replace commas -with semi-colons' so the range query can be passed to the server -(otherwise the query will be split up into two 'Mito:1' and '85779'). In -some cases, a source that supports range queries usually include a range -qualification in its example query. In this case, Jalview will -automatically disable the 'replace commas with semi-colons' option.
-Support for range queries was added in Jalview 2.6

-

If you use the WSDBFetch sequence fetcher services (EMBL, -Uniprot, PDB and PFAM) in work for publication, please cite:

-

Pillai S., Silventoinen V., Kallio K., Senger M., Sobhany S., -Tate J., Velankar S., Golovin A., Henrick K., Rice P., Stoehr P., Lopez -R.
-SOAP-based services provided by the European Bioinformatics Institute.
-Nucleic Acids Res. 33(1):W25-W28 (2005)
-
+

Jalview can retrieve sequences from certain databases using either the +WSDBFetch service provided by the European Bioinformatics Institute, or, since Jalview 2.4, DAS servers capable of the sequence command (configured in DAS settings).

+ The Jalview Sequence Fetcher Dialog Box +

The Sequence Fetcher dialog box can be opened via the "File" + menu on the main desktop in order to retrieve sequences as a new + alignment, or opened via the "File" menu of an existing alignment + to import additional sequences. There may be a short delay when the sequence fetcher is first opened, + whilst Jalview compiles the list of available sequence datasources from the + currently defined DAS server registry.

+

First, select the database you want to retrieve sequences from + by clicking the button labeled 'Select database retrieval source'. If + a database source is already selected, then the button's label will + change to show the currently selected database.

+ Database selection dialog for fetching sequences (introduced in Jalview 2.8) +

Since Jalview 2.8, the + available databases are shown as a tree in a popup dialog box. The + databases are ordered alphabetically, and if there are many sources + for the same type of sequence identifier, they will be grouped + together in a sub-branch branch labeled with the identifier.

+

Once you have selected the sequence database using the popup dialog box, enter + one or more accession ids (as a semi-colon separated list), or press the + "Example" button to paste the example accession for the currently selected database into the retrieval box. + Finally, press "OK" to initiate the retrieval.

+

Specifying chains for PDB IDs + If you are retrieving sequences from the PDB, you can retrieve + specific chains by appending a colon and the chain id to the PDB + id. For example :

 1GAQ:A
+

+

+ Only retrieving part of a sequence DAS sources + (indicated by a "(DAS)") allow a range to be + specified in addition to a sequence ID. To retrieve 50 residues + starting at position 35 in UNIPROT sequence P73137 using the UNIPROT + DAS server, you would enter "'P73137:35,84'.
Full support for DAS range queries was introduced in Jalview 2.8 +

+ +

If you use the WSDBFetch sequence fetcher services (EMBL, Uniprot, PDB, PFAM, and RFAM) + in work for publication, please cite:

+

Pillai S., Silventoinen V., Kallio K., Senger M., Sobhany S., Tate J., Velankar + S., Golovin A., Henrick K., Rice P., Stoehr P., Lopez R.
+ SOAP-based services provided by the European Bioinformatics Institute.
+ Nucleic Acids Res. 33(1):W25-W28 (2005)
+
+