X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=help%2Fhtml%2Ffeatures%2Fseqfetch.html;h=cca5c66d6ba2f9a3da17742a118c46d7d06628cb;hb=a45774ee31d9f35d4eff46d54d7deab719afb092;hp=d8415c7ce69cd856457cffa7dd9aff11bdeda014;hpb=c53aeee9a688f68d64106a968876e542f701fbc6;p=jalview.git diff --git a/help/html/features/seqfetch.html b/help/html/features/seqfetch.html index d8415c7..cca5c66 100755 --- a/help/html/features/seqfetch.html +++ b/help/html/features/seqfetch.html @@ -1,7 +1,7 @@ -Sequence Fetcher + +Sequence Fetcher +

Sequence Fetcher

-

Jalview can retrieve sequences from certain databases using either the -WSDBFetch service provided by the European Bioinformatics Institute, and, since Jalview 2.4, DAS servers capable of the sequence command (configured in DAS settings).

-The Jalview Sequence Fetcher Dialog Box -

The Sequence Fetcher dialog box can be opened via the "File" - menu on the main desktop in order to retrieve sequences as a new - alignment, or opened via the "File" menu of an existing alignment - to import additional sequences. Please note, there will be a short delay when the sequence fetcher is first opened, - whilst Jalview compiles the list of available sequence datasources from the - currently defined DAS server registry. +

Jalview can retrieve sequences from certain databases using +either the WSDBFetch service provided by the European Bioinformatics +Institute, and, since Jalview 2.4, DAS servers capable of the sequence +command (configured in DAS settings).

+The Jalview Sequence Fetcher Dialog Box +

The Sequence Fetcher dialog box can be opened via the +"File" menu on the main desktop in order to retrieve sequences +as a new alignment, or opened via the "File" menu of an +existing alignment to import additional sequences. Please note, there +will be a short delay when the sequence fetcher is first opened, whilst +Jalview compiles the list of available sequence datasources from the +currently defined DAS server registry.

+

First, select the database you want to retrieve sequences from. +Then, enter one or more accession ids (as a semi-colon separated list), +or press the "Example" button to paste the example accession +for the currently selected database into the retrieval box. Finally, +press "OK" to initiate the retrieval.

+

Fetching Individual PDB Chains
+If you are retrieving sequences from the PDB, you can retrieve specific +chains by appending a colon and the chain id to the PDB id. For example +:
+

 1GAQ:A
+

+

Retrieving parts of large sequence records
+When retrieving from DAS sequence sources, coordinate range arguments +can be passed to the server using the standard DAS sequence command +format:

+  <AccessionId>:<start>,<end>
If you know a source +understands this type of query format, then you should untick the +checkbox for 'replace commas with semi-colons' so the range query can be +passed to the server; otherwise, the query will be split into two (e.g +'Mito:1' and '85779' rather than 'Mito:1,85779'). In most cases, +however, a source that supports range queries will include a range +qualification in its example query, and Jalview will then automatically +disable the 'replace commas with semi-colons' option.
+The option to disable the comma to semi-colon translation was +added in Jalview 2.6

+

If you use the WSDBFetch sequence fetcher services (EMBL, +Uniprot, PDB and PFAM) in work for publication, please cite:

+

Pillai S., Silventoinen V., Kallio K., Senger M., Sobhany S., +Tate J., Velankar S., Golovin A., Henrick K., Rice P., Stoehr P., Lopez +R.
+SOAP-based services provided by the European Bioinformatics Institute.
+Nucleic Acids Res. 33(1):W25-W28 (2005)
+

-

First, select the database you want to retrieve sequences from. Then, enter - one or more accession ids (as a semi-colon separated list), or press the - "Example" button to paste the example accession for the currently selected database into the retrieval box. - Finally, press "OK" to initiate the retrieval.

-

- If you are retrieving sequences from the PDB, you can retrieve - specific chains by appending a colon and the chain id to the PDB - id. For example :

 1GAQ:A

When retrieving from DAS sequence sources, - coordinate range arguments can be passed to the server using the standard DAS - sequence command format (:<start>,<end>)

-

If you use the WSDBFetch sequence fetcher services (EMBL, Uniprot, PDB and PFAM) - in work for publication, please cite:

-

Pillai S., Silventoinen V., Kallio K., Senger M., Sobhany S., Tate J., Velankar - S., Golovin A., Henrick K., Rice P., Stoehr P., Lopez R.
- SOAP-based services provided by the European Bioinformatics Institute.
- Nucleic Acids Res. 33(1):W25-W28 (2005)
-
-