Alignment Window Menus

X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=help%2Fhtml%2Fmenus%2FalignmentMenu.html;h=2a65e4319ab97b80da3bbab76b46f3b5cb9e4fb0;hb=67473776389cd0a6024aa3e401819e503970082c;hp=94faed935127949cd7d9a2199361d7b1abdba96f;hpb=865a855a4ca87eadb3e5ff284ed32ed307d9c34b;p=jalview.git diff --git a/help/html/menus/alignmentMenu.html b/help/html/menus/alignmentMenu.html index 94faed9..2a65e43 100755 --- a/help/html/menus/alignmentMenu.html +++ b/help/html/menus/alignmentMenu.html @@ -1,580 +1,639 @@ - + + - -Alignment Window Menus - + --> + Alignment Window Menus - -

- Alignment Window Menus -

File -
- Fetch Sequence
  Shows a - dialog window in which you can retrieve known ids from Uniprot, - EMBL, EMBLCDS, PFAM, Rfam, or PDB database using Web Services provided by the - European Bioinformatics Institute. See Sequence Fetcher .
- Add Sequences
  Add - sequences to the visible alignment from file, URL, or cut & - paste window -
- Reload
  Reloads the - alignment from the original file, if available.
  Warning: - This will delete any edits, analyses and colourings applied since - the alignment was last saved, and cannot be undone. -
- Save (Control S)
  Saves - the alignment to the file it was loaded from (if available), in - the same format, updating the original in place. -
- Save As (Control Shift S)
  Save - the alignment to local file. A file selection window will open, - use the "Files of type:" selection box to determine - which alignment format to save as. -
- Output to Textbox
  The - alignment will be displayed in plain text in a new window, which - you can "Copy and Paste" using the pull down menu, or - your standard operating system copy and paste keys. The output - window also has a "New Window" button - to import the (possibly edited) text as a new alignment.
  - Select the format of the text by selecting one of the following - menu items. -
  - FASTA -
  - MSF -
  - CLUSTAL -
  - BLC -
  - PIR -
  - PFAM -
- Print (Control P)
  Jalview - will print the alignment using the current fonts and colours of - your alignment. If the alignment has annotations visible, these - will be printed below the alignment. If the alignment is wrapped - the number of residues per line of your alignment will depend on - the paper width or your alignment window width, whichever is the - smaller. -
- Export Image
  Creates an - alignment graphic with the current view's annotation, alignment - background colours and group colours. If the alignment is wrapped, the output will also be - wrapped and will have the same visible residue width as the open - alignment. -
  - HTML
    Create a web page from your alignment. -
  - EPS
    Create an Encapsulated Postscript file from - your alignment. -
  - PNG
    Create a Portable Network Graphics file from - your alignment. -
- Export Features
  All - features visible on the alignment can be saved to file or - displayed in a textbox in either Jalview or GFF format -
- Export Annotations
  All - annotations visible on the alignment can be saved to file or - displayed in a textbox in Jalview annotations format. -
- Load Associated Tree
  Jalview - can view trees - stored in the Newick file format, and associate them with the - alignment. Note: the ids of the tree file and your alignment MUST - be the same.
- Load Features / Annotations
  Load - files describing precalculated sequence features or alignment - annotations.
- Close (Control W)
  Close - the alignment window. Make sure you have saved your alignment - before you close - either as a Jalview project or by using the Save - As menu. -
Edit -
- Undo (Control Z)
  This - will undo any edits you make to the alignment. This applies to - insertion or deletion of gaps, cutting residues or sequences from - the alignment or pasting sequences to the current alignment or - sorting the alignment. NOTE: It DOES NOT undo - colour changes, adjustments to group sizes, or changes to the - annotation panel. -
- Redo (Control Y)
  Any - actions which you undo can be redone using redo. -
- Cut (Control X)
  This - will make a copy of the currently selected residues before - removing them from your alignment. Click on a sequence name if you - wish to select a whole sequence.
  Use <CTRL> and X - (<APPLE> and X on MacOSX) to cut. -
- Copy (Control C)
  Copies - the currently selected residues to the system clipboard - you can - also do this by pressing <CTRL> and C (<APPLE> and C - on MacOSX).
  If you try to paste the clipboard contents - to a text editor, you will see the format of the copied residues - FASTA.
- Paste -
  - To New Alignment (Control Shift V)
    - A new alignment window will be created from sequences - previously copied or cut to the system clipboard.
    Use - <CTRL> and <SHIFT> and V(<APPLE> and - <SHIFT;> and and V on MacOSX) to paste. -
  - Add To This Alignment (Control V)
    - Copied sequences from another alignment window can be - added to the current Jalview alignment. -
- Delete (Backspace)
  This - will delete the currently selected residues without copying them - to the clipboard. Like the other edit operations, this can be - undone with Undo. -
- Remove Left (Control L)
  If - the alignment has marked columns, the alignment will be trimmed to - the left of the leftmost marked column. To mark a column, mouse - click the scale bar above the alignment. Click again to unmark a - column, or select "Deselect All" to deselect all - columns.
- Remove Right (Control R)
  If - the alignment has marked columns, the alignment will be trimmed to - the left of the leftmost marked column. To mark a column, mouse - click the scale bar above the alignment. Click again to unmark a - column, or select "Deselect All" to deselect all - columns.
- Remove Empty Columns (Control E)
  - All columns which only contain gap characters - ("-", ".") will be deleted.
  You may - set the default gap character in preferences. -
- Remove All Gaps (Control Shift E)
  - Gap characters ("-", ".") will be - deleted from the selected area of the alignment. If no selection - is made, ALL the gaps in the alignment will be removed.
  - You may set the default gap character in preferences. -
- Remove Redundancy (Control D)
  Selecting - this option brings up a window asking you to select a threshold. - If the percentage identity between any two sequences (under the - current alignment) exceeds this value then one of the sequences - (the shorter) is discarded. Press the "Apply" button to - remove redundant sequences. The "Undo" button will undo - the last redundancy deletion. -
- Pad Gaps
  When selected, - the alignment will be kept at minimal width (so there no empty - columns before or after the first or last aligned residue) and all - sequences will be padded with gap characters to the before and - after their terminating residues.
  This switch is useful - when making a tree using unaligned sequences and when working with - alignment analysis programs which require 'properly aligned - sequences' to be all the same length.
  You may set the - default for Pad Gaps in the preferences. -
Select -
- Find... - (Control F)
  Opens the Find dialog box to - search for residues, sequence name or residue position within the - alignment and create new sequence features from the queries. -
- Select All (Control A)
  Selects - all the sequences and residues in the alignment.
  Use - <CTRL> and A (<APPLE> and A on a MacOSX) to select - all.
- Deselect All (Escape)
  Removes - the current selection box (red dashed box) from the alignment - window. All selected sequences, residues and marked columns will - be deselected.
  Use <ESCAPE> to deselect - all.
- Invert Sequence Selection (Control I)
  - Any sequence ids currently not selected will replace the - current selection. -
- Invert Column Selection (Control Alt I)
  - Any columns currently not selected will replace the current - column selection. -
- Undefine Groups (Control U)
  The - alignment will be reset with no defined groups.
  WARNING: - This cannot be undone. -
- Make Groups
  The currently - selected groups of the alignment will be subdivided according to - the contents of the currently selected region.
  Use this to - subdivide an alignment based on the different combinations of - residues observed at specific positions. (new in jalview 2.5) -
View -
- New View (Control T)
  - Creates a new view from the current alignment view. -
- Expand Views (X)
  Display - each view associated with the alignment in its own alignment - window, allowing several views to be displayed simultaneously. -
- Gather Views (G)
  Each - view associated with the alignment will be displayed within its - own tab on the current alignment window. -
- Show→(all Columns / Sequences / - Sequences and Columns)
  All hidden Columns / - Sequences / Sequences and Columns will be revealed. -
- Hide→(all Columns / Sequences / - Selected Region / All but Selected Region )
  - Hides the all the currently selected Columns / Sequences / Region - or everything but the selected Region. -
- Automatic Scrolling
  When - selected, the view will automatically scroll to display the - highlighted sequence position corresponding to the position under - the mouse pointer in a linked alignment or structure view.
- Show Annotations
  If this - is selected the "Annotation Panel" will be displayed - below the alignment. The default setting is to display the - conservation calculation, quality calculation and consensus values - as bar charts. -
- Autocalculated Annotation
  Settings - for the display of autocalculated annotation. -
  - Apply to all groups
    When - ticked, any modification to the current settings will be applied - to all autocalculated annotation.
  - Show Consensus Histogram
    - Enable or disable the display of the histogram above the - consensus sequence.
  - Show Consensus Logo
    Enable - or disable the display of the Consensus Logo above the consensus - sequence.
  - Normalise Consensus Logo
    - When enabled, scales all logo stacks to the same height, - making it easier to compare symbol diversity in highly variable - regions.
  - Group Conservation
    When - ticked, display a conservation row for all groups (only available - for protein alignments).
  - Apply to all groups
    When - ticked, display a consensus row for all groups.
- Show Sequence Features
  Show - or hide sequence features on this alignment. -
- Seqence - Feature Settings...
  Opens the - Sequence Feature Settings dialog box to control the colour and - display of sequence features on the alignment, and configure and - retrieve features from DAS annotation servers. -

-
Alignment Window Format Menu -
-
Font...
Opens the - "Choose Font" dialog box, in order to change the font of - the display and enable or disable 'smooth fonts' (anti-aliasing) - for faster alignment rendering.
-
Wrap
When ticked, the - alignment display is "wrapped" - to the width of the alignment window. This is useful if your - alignment has only a few sequences to view its full width at once.
- Additional options for display of sequence numbering and scales are - also visible in wrapped layout mode:
-
-
Scale Above
Show the alignment - column position scale.
-
Scale Left
Show the sequence - position for the first aligned residue in each row in the left - column of the alignment.
-
Scale Right
Show the sequence - position for the last aligned residue in each row in the - right-most column of the alignment.
-
Show Sequence Limits
If - this box is selected the sequence name will have the start and - end position of the sequence appended to the name, in the format - NAME/START-END -
-
Right Align Sequence ID
If - this box is selected then the sequence names displayed in the - sequence label area will be aligned against the left-hand edge - of the alignment display, rather than the left-hand edge of the - alignment window. -
-
Show Hidden Markers
When - this box is selected, positions in the alignment where rows and - columns are hidden will be marked by blue arrows. -
-
Boxes
If this is - selected the background of a residue will be coloured using the - selected background colour. Useful if used in conjunction with - "Colour Text." -
-
Text
If this is - selected the residues will be displayed using the standard 1 - character amino acid alphabet. -
-
Colour Text
If this is - selected the residues will be coloured according to the - background colour associated with that residue. The colour is - slightly darker than background so the amino acid symbol remains - visible. -
-
Show Gaps
When this is - selected, gap characters will be displayed as "." or - "-". If unselected, then gap characters will appear as - blank spaces.
You may set the default gap character in - preferences. -
-
Centre Annotation Labels
Select - this to center labels along an annotation row relative to their - associated column (default is off, i.e. left-justified). -
-
Show Unconserved
When - this is selected, all consensus sequence symbols will be - rendered as a '.', highlighting mutations in highly conserved - alignments. -
+ + +
Alignment Window Menus x
-
-
-
+
-
- +
File +
+
Fetch Sequence
+ Shows a dialog window in which you can retrieve known ids +from Uniprot, EMBL, EMBLCDS, PFAM, Rfam, or PDB database using Web +Services provided by the European Bioinformatics Institute. See Sequence Fetcher .
+
Add Sequences
+Add sequences to the visible alignment from file, URL, or cut & +paste window
+
Reload
+Reloads the alignment from the original file, if available.
+ Warning: This will delete any edits, analyses and +colourings applied since the alignment was last saved, and cannot be +undone.
+
Save (Control S)
+Saves the alignment to the file it was loaded from (if available), in +the same format, updating the original in place.
+
Save As (Control Shift S)
+ Save the alignment to local file. A file selection +window will open, use the "Files of type:" selection box to determine +which alignment format to save as. +
+
Output to Textbox
+ The alignment will be displayed in plain text in a +new window, which you can "Copy and Paste" using the pull down menu, or +your standard operating system copy and paste keys. The output window +also has a "New Window" button to import the +(possibly edited) text as a new alignment.
+Select the format of the text by selecting one of the following menu +items. + +
+
FASTA
+
MSF
+
CLUSTAL
+
BLC
+
PIR
+
PFAM
+
+
+
Print (Control P)
+ Jalview will print the alignment using the current +fonts and colours of your alignment. If the alignment has annotations +visible, these will be printed below the alignment. If the alignment is +wrapped the number of residues per line of your alignment will depend +on the paper width or your alignment window width, whichever is the +smaller.
+
Export Image
+Creates an alignment graphic with the current view's annotation, +alignment background colours and group colours. If the alignment is wrapped, the output will also be +wrapped and will have the same visible residue width as the open +alignment. +
+
HTML
+ Create a web page +from your alignment.
+
EPS
+ Create an Encapsulated +Postscript file from your alignment.
+
PNG
+ Create a Portable +Network Graphics file from your alignment.
+
+
+
Export Features
+All features visible on the alignment can be saved to file or displayed +in a textbox in either Jalview or GFF format
+
Export Annotations
+All annotations visible on the alignment can be saved to file or +displayed in a textbox in Jalview annotations format.
+
Load Associated Tree
+ Jalview can view trees stored in the +Newick file format, and associate them with the alignment. Note: the +ids of the tree file and your alignment MUST be the same.
+
Load Features / Annotations
+ Load files describing precalculated sequence features or alignment annotations.
+
Close (Control W)
+ Close the alignment window. Make sure you have saved your +alignment before you close - either as a Jalview project or by using +the Save As menu.
+
+
+
Edit +
+
Undo (Control Z)
+This will undo any edits you make to the alignment. This applies to +insertion or deletion of gaps, cutting residues or sequences from the +alignment or pasting sequences to the current alignment or sorting the +alignment. NOTE: It DOES NOT undo colour changes, +adjustments to group sizes, or changes to the annotation panel.
+
Redo (Control Y)
+ Any actions which you undo can be redone using +redo.
+
Cut (Control X)
+ This will make a copy of the currently selected +residues before removing them from your alignment. Click on a sequence +name if you wish to select a whole sequence.
+Use <CTRL> and X (<APPLE> and X on MacOSX) to cut.
+
Copy (Control C)
+ Copies the currently selected residues to the system +clipboard - you can also do this by pressing <CTRL> and C +(<APPLE> and C on MacOSX).
+If you try to paste the clipboard contents to a text editor, you will +see the format of the copied residues FASTA.
+
Paste +
+
To New Alignment (Control Shift V)
+ A new alignment window will be created from +sequences previously copied or cut to the system clipboard.
+Use <CTRL> and <SHIFT> and V(<APPLE> and +<SHIFT;> and and V on MacOSX) to paste.
+
Add To This Alignment (Control V)
+ Copied sequences from another alignment window +can be added to the current Jalview alignment.
+
+
+
Delete (Backspace)
+ This will delete the currently selected residues +without copying them to the clipboard. Like the other edit operations, +this can be undone with Undo.
+
Remove Left (Control L)
+ If the alignment has marked columns, the alignment +will be trimmed to the left of the leftmost marked column. To mark a +column, mouse click the scale bar above the alignment. Click again to +unmark a column, or select "Deselect All" to deselect all columns.
+
Remove Right (Control R)
+ If the alignment has marked columns, the alignment +will be trimmed to the left of the leftmost marked column. To mark a +column, mouse click the scale bar above the alignment. Click again to +unmark a column, or select "Deselect All" to deselect all columns.
+
Remove Empty Columns (Control E)
+ All columns which only contain gap characters +("-", ".") will be deleted.
+You may set the default gap character in preferences.
+
Remove All Gaps (Control Shift E)
+ Gap characters ("-", ".") will be deleted from the selected +area of the alignment. If no selection is made, ALL the gaps in the +alignment will be removed.
+You may set the default gap character in preferences.
+
Remove Redundancy (Control D)
+ Selecting this option brings up a window asking +you to select a threshold. If the percentage identity between any two +sequences (under the current alignment) exceeds this value then one of +the sequences (the shorter) is discarded. Press the "Apply" button to +remove redundant sequences. The "Undo" button will undo the last +redundancy deletion.
+
Pad Gaps
+ When selected, the alignment will be kept at +minimal width (so there no empty columns before or after the first or +last aligned residue) and all sequences will be padded with gap +characters to the before and after their terminating residues.
+This switch is useful when making a tree using unaligned sequences and +when working with alignment analysis programs which require 'properly +aligned sequences' to be all the same length.
+You may set the default for Pad Gaps in the preferences.
+
+
+
Select +
+
Find... (Control F) +
+Opens the Find dialog box to search for residues, sequence name or +residue position within the alignment and create new sequence features +from the queries.
+
Select All (Control A)
+ Selects all the sequences and residues in the +alignment.
+Use <CTRL> and A (<APPLE> and A on a MacOSX) to select all.
+
Deselect All (Escape)
+ Removes the current selection box (red dashed box) +from the alignment window. All selected sequences, residues and marked +columns will be deselected.
+Use <ESCAPE> to deselect all.
+
Invert Sequence Selection (Control I)
+ Any sequence ids currently not selected will +replace the current selection.
+
Invert Column Selection (Control Alt I)
+ Any columns currently not selected will replace +the current column selection.
+
Create Group (Control G)
+ Create a group containing the currently selected +sequences.
+
Remove Group (Shift Control G)
+ Ungroup the currently selected sequence group. +(Create/Remove group new in Jalview 2.8.1)
+
Make Groups for selection
+ The currently selected groups of the alignment +will be subdivided according to the contents of the currently selected +region.
+Use this to subdivide an alignment based on the different combinations +of residues observed at specific positions. (new in jalview 2.5)
+
Undefine Groups (Control U)
+ The alignment will be reset with no defined groups.
+ WARNING: This cannot be undone.
+
+
+
View +
+
New View (Control T)
+Creates a new view from the current alignment view.
+
Expand Views (X)
+Display each view associated with the alignment in its own alignment +window, allowing several views to be displayed simultaneously.
+
Gather Views (G)
+Each view associated with the alignment will be displayed within its +own tab on the current alignment window.
+
Show→(all Columns / Sequences / Sequences and Columns)
+All hidden Columns / Sequences / Sequences and Columns will be +revealed.
+
Hide→(all Columns / Sequences / Selected Region / All +but Selected Region )
+Hides the all the currently selected Columns / Sequences / Region or +everything but the selected Region.
+
Automatic Scrolling
+ When selected, the view will automatically scroll +to display the highlighted sequence position corresponding to the +position under the mouse pointer in a linked alignment or structure +view.
+
Show Annotations
+ If this is selected the "Annotation Panel" will be +displayed below the alignment. The default setting is to display the +conservation calculation, quality calculation and consensus values as +bar charts.
+
Autocalculated Annotation
+ Settings for the display of autocalculated +annotation. +
+
Apply to all groups
+ When ticked, any modification to the current +settings will be applied to all autocalculated annotation.
+
Show Consensus Histogram
+ Enable or disable the display of the +histogram above the consensus sequence.
+
Show Consensus Logo
+ Enable or disable the display of the +Consensus Logo above the consensus sequence.
+
Normalise Consensus Logo
+ When enabled, scales all logo stacks to the +same height, making it easier to compare symbol diversity in highly +variable regions.
+
Group Conservation
+ When ticked, display a conservation row for +all groups (only available for protein alignments).
+
Apply to all groups
+ When ticked, display a consensus row for all +groups.
+
+
+
Show Sequence Features
+ Show or hide sequence features on this alignment.
+
Seqence +Feature Settings...
+ Opens the Sequence Feature Settings dialog box to control +the colour and display of sequence features on the alignment, and +configure and retrieve features from DAS annotation servers.
+ +
Sequence ID Tooltip (application +only)
+This submenu's options allow the inclusion or exclusion of +non-positional sequence features or database cross references from the +tooltip shown when the mouse hovers over the sequence ID panel.
+ +
Alignment Properties...
+ Displays some simple statistics computed for the +current alignment view and any named properties defined on the whole +alignment.
+ +
Overview +Window
+ A scaled version of the alignment will be +displayed in a small window. A red box will indicate the currently +visible area of the alignment. Move the visible region using the mouse. +
+ +
+
+ +
Alignment Window Format Menu +
+ +
Font...
+ Opens the "Choose Font" dialog box, in order to +change the font of the display and enable or disable 'smooth fonts' +(anti-aliasing) for faster alignment rendering.
+ +
Wrap
+ When ticked, the alignment display is "wrapped" to the width of the +alignment window. This is useful if your alignment has only a few +sequences to view its full width at once.
+Additional options for display of sequence numbering and scales are +also visible in wrapped layout mode:
+ +
+ +
Scale Above
+ Show the alignment column position scale.
+ +
Scale Left
+ Show the sequence position for the first aligned +residue in each row in the left column of the alignment.
+ +
Scale Right
+ Show the sequence position for the last aligned +residue in each row in the right-most column of the alignment.
+ +
Show Sequence Limits
+ If this box is selected the sequence name will +have the start and end position of the sequence appended to the name, +in the format NAME/START-END
+ +
Right Align Sequence ID
+ If this box is selected then the sequence +names displayed in the sequence label area will be aligned against the +left-hand edge of the alignment display, rather than the left-hand edge +of the alignment window.
+ +
Show Hidden Markers
+ When this box is selected, positions in the +alignment where rows and columns are hidden will be marked by blue +arrows.
+ +
Boxes
+If this is selected the background of a residue will be coloured using +the selected background colour. Useful if used in conjunction with +"Colour Text."
+ +
Text
+ If this is selected the residues will be +displayed using the standard 1 character amino acid alphabet.
+ +
Colour Text
+ If this is selected the residues will be +coloured according to the background colour associated with that +residue. The colour is slightly darker than background so the amino +acid symbol remains visible.
+ +
Show Gaps
+ When this is selected, gap characters will be +displayed as "." or "-". If unselected, then gap characters will appear +as blank spaces.
+You may set the default gap character in preferences.
+ +
Centre Annotation Labels
+ Select this to center labels along an +annotation row relative to their associated column (default is off, +i.e. left-justified).
+ +
Show Unconserved
+ When this is selected, all consensus sequence +symbols will be rendered as a '.', highlighting mutations in highly +conserved alignments.
+ +
+
+ +
+
+ +

Colour -

Apply Colour To All Groups
If - this is selected, any changes made to the background colour will - be applied to all currently defined groups.
-
Colour - Text...
Opens the Colour Text dialog box to - set a different text colour for light and dark background, and the - intensity threshold for transition between them. -
Colour Scheme options: None, ClustalX, - Blosum62 Score, Percentage Identity, Zappo, Taylor, - Hydrophobicity, Helix Propensity, Strand Propensity, Turn - Propensity, Buried Index, Nucleotide, Purine/Pyrimidine, User Defined
See - colours for a - description of all colour schemes.
By Conservation
See Colouring by - Conservation.
Modify Conservation Threshold
Use - this to display the conservation threshold slider window. Useful - if the window has been closed, or if the 'by conservation' option - appears to be doing nothing!
Above Identity Threshold
See - Above Percentage - Identity .
-
Modify Identity Threshold
Use - this to set the threshold value for colouring above Identity. - Useful if the window has been closed.
-
By Annotation
Colours - the alignment on a per-column value from a specified annotation. - See Annotation - Colouring.
By RNA Helices
- Colours the helices of an RNA alignment loaded from a Stockholm file. See - RNA Helices - Colouring.
-

Calculate -

Sort -
- by ID
  This will sort - the sequences according to sequence name. If the sort is - repeated, the order of the sorted sequences will be inverted. -
- by Length
  This will - sort the sequences according to their length (excluding gap - characters). If the sort is repeated, the order of the sorted - sequences will be inverted.
- by Group
  This - will sort the sequences according to sequence name. If the sort - is repeated, the order of the sorted sequences will be inverted. -
- by Pairwise Identity
  This - will sort the selected sequences by their percentage identity to - the consensus sequence. The most similar sequence is put at the - top.
- The Sort - menu will have some additional options if you have just done a - multiple alignment calculation, or opened a tree viewer window.
  -
-
Calculate Tree
Functions - for calculating trees on the alignment or the currently selected - region. See calculating - trees. -
- Average Distance Using % Identity
- Neighbour Joining Using % Identity
- Average Distance Using Blosum62
- Neighbour Joining Using Blosum62
  -
-
Pairwise Alignments
Applies - Smith and Waterman algorithm to selected sequences. See pairwise alignments.
-
Principal Component Analysis
Shows - a spatial clustering of the sequences based on the BLOSUM62 scores - in the alignment. See Principal - Component Analysis.
-
Extract Scores ... (optional)
This - option is only visible if Jalview detects one or more white-space - separated values in the description line of the alignment - sequences.
When selected, these numbers are parsed into - sequence associated annotation which can then be used to sort the - alignment via the Sort by→Score menu.
-
Autocalculate Consensus
For - large alignments it can be useful to deselect "Autocalculate - Consensus" when editing. This prevents the sometimes lengthy - calculations performed after each sequence edit.
-
Sort With New Tree
When - enabled, Jalview will automatically sort the alignment when a new - tree is calculated or loaded onto it.

+ +

Colour +

Apply Colour To All Groups
+ If this is selected, any changes made to the +background colour will be applied to all currently defined groups.
+

Colour +Text...
+Opens the Colour Text dialog box to set a different text colour for +light and dark background, and the intensity threshold for transition +between them.

Colour Scheme options: None, ClustalX, +Blosum62 Score, Percentage Identity, Zappo, Taylor, Hydrophobicity, +Helix Propensity, Strand Propensity, Turn Propensity, Buried Index, +Nucleotide, Purine/Pyrimidine, User Defined
+ See colours +for a description of all colour schemes.
+

By Conservation
+ See Colouring +by Conservation.
+

Modify Conservation Threshold
+ Use this to display the conservation threshold +slider window. Useful if the window has been closed, or if the 'by +conservation' option appears to be doing nothing!
+

Above Identity Threshold
+ See Above +Percentage Identity .
+

Modify Identity Threshold
+ Use this to set the threshold value for colouring +above Identity. Useful if the window has been closed.
+

By Annotation
+ Colours the alignment on a per-column value from a specified +annotation. See Annotation +Colouring.
+

By RNA Helices
+ Colours the helices of an RNA alignment loaded from a +Stockholm file. See RNA +Helices Colouring.
+

Web Service Menu
This menu - is dynamic, and may contain user-defined web service entries in - addition to any of the following ones: -

Fetch DB References
This - will use any of the database services that Jalview is aware of - (e.g. DAS sequence servers and the WSDBFetch service provided by - the EBI) to verify the sequence and retrieve all database cross - references and PDB ids associated with all or just the selected - sequences in the alignment.
'Standard Databases' will check - sequences against the EBI databases plus any active DAS sequence - sources, or you can verify against a specific source from one of - the sub-menus.
Envision2 Services
Submits one or - more sequences, sequence IDs or database references to analysis - workflows provided by the EnVision2 web - application. This allows Jalview users to easily access the EnCore - network of databases and analysis services developed by members of - ENFIN.

Selecting items from the following submenus will start a - remote service on compute facilities at the University of Dundee, or - elsewhere. You need a continuous network connection in order to use - these services through Jalview. -

Alignment
Align the currently - selected sequences or all sequences in the alignment, or re-align - unaligned sequences to the aligned sequences. Entries in this menu - provide access to the various alignment programs supported by JABAWS. See the Multiple Sequence - Alignment webservice client entry for more information.
Secondary Structure Prediction -
- JPred Secondary Structure Prediction
  - Secondary structure prediction by network consensus. See - the Jpred3 client entry for - more information. The behaviour of this calculation depends on - the current selection: -
  -
  If nothing is selected, and the displayed sequences - appear to be aligned, then a JNet prediction will be run for - the first sequence in the alignment, using the current - alignment. Otherwise the first sequence will be submitted for - prediction.
  -
  If just one sequence (or a region on one sequence) has - been selected, it will be submitted to the automatic JNet - prediction server for homolog detection and prediction.
  -
  If a set of sequences are selected, and they appear to - be aligned, then the alignment will be used for a Jnet - prediction on the first sequence in the set - (that is, the one that appears first in the alignment window). -
  -
  -
Analysis
-
- Multi-Harmony
  Performs - functional residue analysis on a protein family alignment with - sub-families defined on it. See the Multi-Harmony service entry for more - information. -

- + +

Calculate +

Sort +
- by ID
  +This will sort the sequences according to sequence name. If the sort is +repeated, the order of the sorted sequences will be inverted.
- by Length
  +This will sort the sequences according to their length (excluding gap +characters). If the sort is repeated, the order of the sorted sequences +will be inverted.
- by Group
  + This will sort the sequences according to +sequence name. If the sort is repeated, the order of the sorted +sequences will be inverted.
- by Pairwise Identity
  + This will sort the selected sequences by their +percentage identity to the consensus sequence. The most similar +sequence is put at the top.
- The Sort +menu will have some additional options if you have just done a +multiple alignment calculation, or opened a tree viewer window.
  +
+

Calculate Tree
+ Functions for calculating trees on the alignment or the +currently selected region. See calculating +trees. +
- Average Distance Using % Identity
- Neighbour Joining Using % Identity
- Average Distance Using Blosum62
- Neighbour Joining Using Blosum62
  +
+ Note: Since Version 2.8.1, a number of +additional similarity measures for tree calculation are provided in +this menu.

Pairwise Alignments
+ Applies Smith and Waterman algorithm to selected sequences. +See pairwise alignments.
+

Principal Component Analysis
+ Shows a spatial clustering of the sequences based on +similarity scores calculated with the alignment. See Principal Component Analysis. +
+

Extract Scores ... (optional)
+ This option is only visible if Jalview detects one or more +white-space separated values in the description line of the alignment +sequences.
+When selected, these numbers are parsed into sequence associated +annotation which can then be used to sort the alignment via the Sort +by→Score menu.
+

Autocalculate Consensus
+ For large alignments it can be useful to deselect +"Autocalculate Consensus" when editing. This prevents the sometimes +lengthy calculations performed after each sequence edit.
+

Sort With New Tree
+ When enabled, Jalview will automatically sort the alignment +when a new tree is calculated or loaded onto it.
+

Show Flanking Regions
+ Opens a new alignment window showing any additional sequence +data either side of the current alignment. Useful in conjunction with +'Fetch Database References' when the 'Trim Retrieved Sequences' option +is disabled to retrieve full length sequences for a set of aligned +peptides.

- - + +

Web Service Menu
+ This menu is dynamic, and may contain user-defined web service +entries in addition to any of the following ones: +

Fetch DB References
+ This submenu contains options for accessing any of the +database services that Jalview is aware of (e.g. DAS sequence servers +and the WSDBFetch service provided by the EBI) to verify sequence +start/end positions and retrieve all database cross references and PDB +ids associated with all or just the selected sequences in the +alignment. +
- 'Trim Retrieved Sequences' - when checked, +Jalview will discard any additional sequence data for accessions +associated with sequences in the alignment.
  + Note: Disabling this could cause out of memory errors +when working with genomic sequence records !
  + Added in Jalview 2.8.1
- 'Standard Databases' will check sequences +against the EBI databases plus any active DAS sequence sources<
+ Other sub-menus allow you to pick a specific +source to query - sources are listed alphabetically according to their +nickname.
+

+ +

Selecting items from the following submenus will start +a remote service on compute facilities at the University of Dundee, or +elsewhere. You need a continuous network connection in order to use +these services through Jalview.

+ +

Alignment
+ Align the currently selected sequences or all sequences in +the alignment, or re-align unaligned sequences to the aligned +sequences. Entries in this menu provide access to the various alignment +programs supported by JABAWS. +See the Multiple Sequence +Alignment webservice client entry for more information.

Secondary Structure Prediction +
- JPred Secondary Structure Prediction
  + Secondary structure prediction by network consensus. See +the Jpred3 client entry for +more information. The behaviour of this calculation depends on the +current selection: +
  - If nothing is selected, and the +displayed sequences appear to be aligned, then a JNet prediction will +be run for the first sequence in the alignment, using the current +alignment. Otherwise the first sequence will be submitted for +prediction.
  - If just one sequence (or a region on +one sequence) has been selected, it will be submitted to the automatic +JNet prediction server for homolog detection and prediction.
  - If a set of sequences are selected, and +they appear to be aligned, then the alignment will be used for a Jnet +prediction on the first sequence in the set (that is, +the one that appears first in the alignment window).
  +
+

Analysis
+ +
- Multi-Harmony
  + Performs functional residue analysis on a protein family +alignment with sub-families defined on it. See the Multi-Harmony service entry for +more information.
+

+ + + \ No newline at end of file