X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=help%2Fhtml%2Fna%2Findex.html;h=2d5aa08dbd18d84ceeb1219968c318aefbe27a3e;hb=37de9310bec3501cbc6381e0c3dcb282fcaad812;hp=11549fd61d157c2cefeacbaca9fe420666bbb392;hpb=ad15cff29620f960119f80176f1fd443da9f6763;p=jalview.git diff --git a/help/html/na/index.html b/help/html/na/index.html index 11549fd..2d5aa08 100644 --- a/help/html/na/index.html +++ b/help/html/na/index.html @@ -32,68 +32,92 @@ td { -

- Nucleic Acid Support -

- Colour Schemes -

Jalview has color schemes for nucleic acid based sequences, - ability to fetch sequences from RFAM and RNA secondary structure - coloring

- Information on the Nucleotide - colour scheme and - Purine/Pyrimidine colour scheme are available under the Colour Menu. - See Colour Schemes. -

- RNA Support -

- Jalview supports annotation of RNA sequences with secondary structure - information. You can interactively - create and edit RNA - secondary structure annotation rows, or import data in the following - way: -

RFAM - Sequences can be fetched from the RFAM database - by accession number or ID.
Stockholm files - WUSS (or VIENNA) dot-bracket - notation found in the secondary structure annotation line will be - imported as sequence or alignment associated secondary structure - annotation.
Clustal files - certain RNA alignment programs, such - as LocaRNA - output consensus RNA secondary structure lines in the line normally - reserved for the Clustal consensus line in a clustal file.
RNAML Jalview can import RNAML files containing sequences and extended secondary structure annotation derived from RNA 3D structure

- RNA Secondary Structure Visualization and Analysis
- If a sequence or RNA alignment has secondary structure information, - the alignment will have a secondary structure line shown below it, and - a number of additional options become available: -

RNA - Helix colouring - highlights columns of alignment involved in - particular RNA helices.
Base Pair - Conservation Analysis - shown as a histogram and base-pair logo - below the alignment.
2D Structure - Visualization in VARNA - allows linked viewing of the consensus or - an individual sequence's structure

Pseudo-knots
- Jalview 2.8.2 introduced limited support for working with structures including pseudoknots. Where possible, extended WUSS symbols (e.g. different types of parentheses, or upper and lower case letters) are preserved when parsing RNA structure annotation and will be shaded differently when displayed in the structure.
- Extended WUSS annotation is also employed to distinguish different base pair interactions obtained from RNAML files.

- -

Limitations when working with RNA in Jalview
- Currently, Jalview is not able to export RNA secondary structure annotation in any format other than Jalview annotation -
- Jalview's RNA handling capabilities were introduced in v2.8 -

+ Nucleic Acid Support +

+ Colour Schemes +

Jalview has color schemes for nucleic acid based sequences, + ability to fetch sequences from RFAM and RNA secondary structure + coloring

+ Information on the Nucleotide + colour scheme and + Purine/Pyrimidine colour scheme are available under the Colour + Menu. See Colour Schemes. +

+ RNA Support +

+ Jalview supports annotation of RNA sequences with secondary structure + information. You can interactively + create and edit RNA + secondary structure annotation rows, or import data in the following + way: +

RFAM - Sequences can be fetched from the RFAM + database by accession number or ID.
Stockholm files - WUSS (or VIENNA) dot-bracket + notation found in the secondary structure annotation line will be + imported as sequence or alignment associated secondary structure + annotation.
Clustal files - certain RNA alignment programs, + such as LocaRNA + output consensus RNA secondary structure lines in the line + normally reserved for the Clustal consensus line in a clustal + file.
RNAML Jalview can import RNAML files containing + sequences and extended secondary structure annotation derived from + RNA 3D structure

+ RNA Secondary Structure Visualization and Analysis
+ If a sequence or RNA alignment has secondary structure information, + the alignment will have a secondary structure line shown below it, + and a number of additional options become available: +

RNA + Helix colouring - highlights columns of alignment involved in + particular RNA helices, Uses the first displayed secondary + structure annotation.
Base + Pair Conservation Analysis - shown as a histogram and base-pair + logo below the alignment. Uses the first displayed secondary + structure annotation row.
2D Structure + Visualization in VARNA - allows linked viewing of the consensus + or an individual sequence's structure. Accessed via the Sequence + ID popup menu.
per sequence secondary structure + annotation
Sequence associated secondary structure + annotation imported via stockholm, PDB files, or other sources can + be shown on the alignment with the Colours→By + Annotation dialog box. Colours are assigned according to RNA + helix topology number (number of distinct nested helices). + Alignments can also be sorted by RNA helix secondary structure + topology number, via the Calculations→Sort→By + Annotation→Secondary Structure option (only present when + per-sequence secondary structure is available).

+ Pseudo-knots
Jalview 2.8.2 introduced + limited support for working with structures including pseudoknots. + Where possible, extended WUSS symbols (e.g. different types of + parentheses, or upper and lower case letters) are preserved when + parsing RNA structure annotation and will be shaded differently when + displayed in the structure.
Extended WUSS annotation is also + employed to distinguish different base pair interactions obtained + from RNAML files. +

+ +

+ Limitations when working with RNA in Jalview
+ Currently, Jalview is not able to export RNA secondary structure + annotation in any format other than Jalview annotation
Jalview's + RNA handling capabilities were introduced in v2.8 +