Release History

- General -

Improved memory usage: sparse arrays used for all consensus calculations
Updated Jmol shipped with applet and desktop to 14.6.4

+ Faster and more efficient management and + rendering of sequence features +
+ More reliable Ensembl fetching with HTTP + 429 rate limit request hander +
+ Structure views don't get updated unless + their colours have changed +

Application

Example groovy script for generating a matrix of percent identity scores for current alignment.

Sequence ID tool tips have been tamed (databases sorted alphabetically, abridged ID sets)
New replacement token for creating URLs just from database cross references. Users with custom links will receive a warning dialog asking them to update their preferences.
Cancel button and escape listener on dialog warning user about disconnecting Jalview from a Chimera session
Jalview's chimera control window closes if the Chimera it is connected to is shut down
Custom URL links for database cross-references are matched to database name regardless of case
Select highlighted columns menu item and keystroke (B) to mark columns containing highlighted regions from structure selections or results of a Find operation
Command line option for batch-generation of HTML pages rendering alignment data with the BioJS MSAviewer
Structures with whitespace chainCode cannot be viewed in Chimera
Protein annotation panel too high in CDS/Protein view +
Can't edit the query after the server error warning icon is shown in Uniprot and PDB Free Text Search Dialogs +

- Applet + + + + +

+ 2.10.2b2
+ 2/10/2017 +

+ +

+ New features in Jalview Desktop

+ Uniprot Sequence Fetcher now uses web API at uniprot.org +
HTTPS used for all connections to ebi.ac.uk +

- Build and deployment +

+ + + +

+ 2.10.2b1
+ 7/9/2017 +

+ +

Updated Jalview's Certum code signing certificate for 2016-2017
+ Show gaps in overview window by colouring + in grey (sequences used to be coloured grey, and gaps were + white) +
+ Overview tab in Jalview Desktop + Preferences +
+ Overview updates immediately on increase + in size and progress bar shown as higher resolution + overview is recalculated +

+ +

+ Overview window redraws every hidden + column region row by row +
+ duplicate protein sequences shown after + retrieving Ensembl crossrefs for sequences from Uniprot +
+ Overview window throws NPE if show boxes + format setting is unticked +
+ Groups are coloured wrongly in overview + if group has show boxes format setting unticked +
+ Redraw problems when + autoscrolling whilst dragging current selection group to + include sequences and columns not currently displayed +
+ Not all chains are mapped when multimeric + assemblies are imported via CIF file +
+ Gap colour in custom colourscheme is not + displayed when threshold or conservation colouring is also + enabled. +
+ JABAWS 2.2 services report wrong JABAWS + server version +
+ Jalview continues to scroll after + dragging a selected region off the visible region of the + alignment +
+ Cannot apply annotation based + colourscheme to all groups in a view +
+ IDs don't line up with sequences + initially after font size change using the Font chooser or + middle-mouse zoom +

+ 2.10.2
17/8/2017 +

- General +

+ Calculations

Columns with more than one modal residue are not coloured or thresholded according to percent identity (first observed in Jalview 2.8.2)
Threonine incorrectly reported as not hydrophobic
Updates to documentation pages (above PID threshold, amino acid properties)
Lower case residues in sequences are not reported as mapped to residues in a structure file in the View Mapping report
Identical features with non-numeric scores could be added multiple times to a sequence
+ Occupancy annotation row shows number of + ungapped positions in each column of the alignment. +
+ Tree/PCA calculation menu items merged to + a calculation dialog box +
+ Revised implementation of PCA for speed + and memory efficiency (~30x faster) +
+ Revised implementation of sequence + similarity scores as used by Tree, PCA, Shading Consensus + and other calculations +
+ Score matrices are stored as resource + files within the Jalview codebase +
+ Trees computed on Sequence Feature + Similarity may have different topology due to increased + precision +

- Application + Rendering +

+ More robust colours and shader + model for alignments and groups +
+ Custom shading schemes created via groovy + scripts +

+ Overview +

+ Efficiency improvements for interacting + with alignment and overview windows +
+ Scrolling of wrapped alignment views via + overview +
+ Hidden columns and sequences can be + omitted in Overview +
+ Click-drag in visible area allows fine + adjustment of visible position +

+ + Data import/export +

+ Posterior probability annotation from + Stockholm files imported as sequence associated annotation +
+ More robust per-sequence positional + annotation input/output via stockholm flatfile +
+ Sequence names don't include file + extension when importing structure files without embedded + names or PDB accessions +
+ Drag and drop load of AAIndex and NCBI + format sequence substitution matrices +

+ User Interface +

+ Experimental Features Checkbox in + Desktop's Tools menu to hide or show untested features in + the application. +
+ Linked scrolling of CDS/Protein views + via Overview or sequence motif search operations +
+ Amend sequence features dialog box can be + opened by double clicking gaps within sequence feature + extent +
+ Status bar message shown when not enough + aligned positions were available to create a 3D structure + superposition. +

+ 3D Structure +

+ Hidden regions in alignment views are not + coloured in linked structure views +
+ Faster Chimera/Jalview communication by + file-based command exchange +
+ Structure chooser automatically shows + Cached Structures rather than querying the PDBe if + structures are already available for sequences +
+ Structures imported via URL are cached in + the Jalview project rather than downloaded again when the + project is reopened. +
+ New entries in the Chimera menu + to transfer Chimera's structure attributes as Jalview + features, and vice-versa (Experimental + Feature) +

+ Web Services +

+ Updated JABAWS client to v2.2 +
+ Filter non-standard amino acids and + nucleotides when submitting to AACon and other MSA + Analysis services +
+ URLs for viewing database + cross-references provided by identifiers.org and the + EMBL-EBI's MIRIAM DB +

+ + Scripting +

+ FileFormatI interface for describing and + identifying file formats (instead of String constants) +
+ FeatureCounter script refactored for + efficiency when counting all displayed features (not + backwards compatible with 2.10.1) +

+ Example files +

+ Graduated feature colour style example + included in the example feature file +

+ Documentation +

+ Release notes reformatted for readability + with the built-in Java help viewer +
+ Find documentation updated with 'search + sequence description' option +

+ Test Suite +

+ External service integration tests for + Uniprot REST Free Text Search Client +
+ Added PrivilegedAccessor to test suite +
+ Prevent or clear modal dialogs raised + during tests +

+ Calculations +

+ Fixed incorrect value in BLOSUM 62 score + matrix - C->R should be '-3'
Old matrix restored + with this one-line groovy script:
jalview.analysis.scoremodels.ScoreModels.instance.BLOSUM62.@matrix[4][1]=3 +
Fixed + Jalview's treatment of gaps in PCA and substitution matrix + based Tree calculations.

In earlier versions + of Jalview, gaps matching gaps were penalised, and gaps + matching non-gaps penalised even more. In the PCA + calculation, gaps were actually treated as non-gaps - so + different costs were applied, which meant Jalview's PCAs + were different to those produced by SeqSpace.
Jalview + now treats gaps in the same way as SeqSpace (ie it scores + them as 0).

Enter the following in the + Groovy console to restore pre-2.10.2 behaviour:
+ jalview.analysis.scoremodels.ScoreMatrix.scoreGapAsAny=true + // for 2.10.1 mode
+ jalview.analysis.scoremodels.ScoreMatrix.scoreGapAsAny=false + // to restore 2.10.2 mode

Note: + these settings will affect all subsequent tree and PCA + calculations (not recommended)

+ Fixed off-by-one bug that affected + scaling of branch lengths for trees computed using + Sequence Feature Similarity. +

+ PCA calculation could hang when + generating output report when working with highly + redundant alignments +

+ Sort by features includes features to + right of selected region when gaps present on right-hand + boundary +

+ User Interface +

+ Reopening Colour by annotation dialog + doesn't reselect a specific sequence's associated + annotation after it was used for colouring a view +
+ Current selection lost if popup menu + opened on a region of alignment without groups +
+ Popup menu not always shown for regions + of an alignment with overlapping groups +
+ Finder double counts if both a sequence's + name and description match +
+ Hiding column selection containing two + hidden regions results in incorrect hidden regions +
+ 'Apply to all groups' setting when + changing colour does not apply Conservation slider value + to all groups +
+ Percentage identity and conservation menu + items do not show a tick or allow shading to be disabled +
+ Conservation shading or PID threshold + lost when base colourscheme changed if slider not visible +
+ Sequence features shown in tooltip for + gaps before start of features +
+ Graduated feature colour threshold not + restored to UI when feature colour is edited +
+ Vertical scrollbar jumps one page-width at + a time when scrolling vertically in wrapped mode. +
+ Structure and alignment overview update + as graduate feature colour settings are modified via the + dialog box +
+ Overview window doesn't always update + when a group defined on the alignment is resized +
+ Mouseovers on left/right scale region in + wrapped view result in positional status updates +
+ Status bar doesn't show position for + ambiguous amino acid and nucleotide symbols +
+ Copy consensus sequence failed if + alignment included gapped columns +
+ Minimum size set for Jalview windows so + widgets don't permanently disappear +
+ Cannot select or filter quantitative + annotation that are shown only as column labels (e.g. + T-Coffee column reliability scores) +
+ Exception thrown if trying to create a + sequence feature on gaps only +
+ Features created with 'New feature' + button from a Find inherit previously defined feature type + rather than the Find query string +
+ incorrect title in output window when + exporting tree calculated in Jalview +
+ Hiding sequences at bottom of alignment + and then revealing them reorders sequences on the + alignment +
+ Group panel in sequence feature settings + doesn't update to reflect available set of groups after + interactively adding or modifying features +
+ Sequence Database chooser unusable on + Linux +
+ Hide insertions in PopUp->Selection menu + only excluded gaps in current sequence and ignored + selection. +

+ Rendering +

+ Overview window visible region moves + erratically when hidden rows or columns are present +
+ Per-residue colourschemes applied via the + Structure Viewer's colour menu don't correspond to + sequence colouring +
+ Protein specific colours only offered in + colour and group colour menu for protein alignments +
+ Colour threshold slider doesn't update to + reflect currently selected view or group's shading + thresholds +
+ Feature colour thresholds not respected + when rendered on overview and structures when opacity at + 100% +
+ User defined gap colour not shown in + overview when features overlaid on alignment +

+ Data import/export +

+ Very large alignments take a long time to + load +
+ Per-sequence RNA secondary structures + added after a sequence was imported are not written to + Stockholm File +
+ WUSS notation for simple pseudoknots lost + when importing RNA secondary structure via Stockholm +
+ Secondary structure arrows for [] and {} + not shown in correct direction for simple pseudoknots +
+ Cannot configure feature colours + with lightGray or darkGray via features file (but can + specify lightgray) +
+ Above PID colour threshold not recovered + when alignment view imported from project +
+ No mappings generated between + structure and sequences extracted from structure files + imported via URL and viewed in Jmol +
+ Structures loaded via URL are saved in + Jalview Projects rather than fetched via URL again when + the project is loaded and the structure viewed +

+ Web Services

Custom URL links for specific database names without regular expressions also offer links from Sequence ID
Removing a single configured link in the URL links pane in Connections preferences doesn't actually update Jalview configuration
CTRL-Click on a selected region to open the alignment area popup menu doesn't work on El-Capitan
Jalview doesn't offer to associate mmCIF files with similarly named sequences if dropped onto the alignment
Additional mappings are shown for PDB entries where more chains exist in the PDB accession than are reported in the SIFTS file
Certain structures do not get mapped to the structure view when displayed with Chimera
No chains shown in the Chimera view panel's View->Show Chains submenu
Export as HTML with embedded SVG doesn't work for wrapped alignment views
- Disulphide bond features shown as two - highlighted residues rather than a range in linked - structure views, and treated correctly when selecting and - computing trees from features -
Rename UI components for running JPred predictions from 'JNet' to 'JPred'
Export as PNG or SVG is corrupted when annotation panel vertical scroll is not at first annotation row
+ EnsemblGenomes example failing after + release of Ensembl v.88 +
+ Proxy server address and port always + appear enabled in Preferences->Connections +
+ DAS registry not found exceptions + removed from console output +
+ Cannot retrieve protein products from + Ensembl by Peptide ID +
+ Incorrect PDB-Uniprot mappings + created from SIFTs, and spurious 'Couldn't open structure + in Chimera' errors raised after April 2017 update (problem + due to 'null' string rather than empty string used for + residues with no corresponding PDB mapping). +

+ Application UI +

+ User Defined Colours not added to Colour + menu +
+ Easier creation of colours for all 'Lower + case' residues (button in colourscheme editor debugged and + new documentation and tooltips added) +
+ Text colour threshold's 'Cancel' button + doesn't restore group-specific text colour thresholds +
+ Feature settings panel does not update as + new features are added to alignment +
+ Cancel in feature settings reverts + changes to feature colours via the Amend features dialog +
+ Null pointer exception when attempting to + edit graduated feature colour via amend features dialog + box +
+ Structure viewer's View -> Colour By view + selection menu changes colours of alignment views +
+ Spurious exceptions in console raised + from alignment calculation workers after alignment has + been closed +
+ Typo in selection popup menu - Create + groups now 'Create Group' +
+ CMD/CTRL and G or Shift G for + Create/Undefine group doesn't always work +
+ Tree Viewer's Print Dialog doesn't get + shown again after pressing 'Cancel' +
+ Trackpad horizontal scroll gesture + adjusts start position in wrap mode +
+ Status bar doesn't show positions for + ambiguous amino acids +
+ cDNA Consensus annotation not shown in + CDS/Protein view after CDS sequences added for aligned + proteins +
+ User defined colourschemes called 'User + Defined' don't appear in Colours menu +

Applet

+ Switching between Nucleotide and Protein + score models doesn't always result in an updated PCA plot +
+ Features not rendered as transparent on + overview or linked structure view +
+ Colour group by conservation doesn't + work (since 2.8) +
+ Hitting Cancel after applying + user-defined colourscheme doesn't restore original + colourscheme +

- Build and deployment + Test Suite

Failing/passing unit tests
+ Unit test failure: + jalview.ws.jabaws.RNAStructExportImport setup fails +
+ Unit test failure: + jalview.ws.sifts.SiftsClientTest due to compatibility + problems with deep array comparison equality asserts in + successive versions of TestNG +
+ Relocated StructureChooserTest and + ParameterUtilsTest Unit tests to Network suite +

New Known Issues

+ Protein/CDS view scrolling not always in + phase after a sequence motif find operation +
+ Importing annotation file with rows + containing just upper and lower case letters are + interpreted as WUSS RNA secondary structure symbols +
+ Cannot load and display Newick trees + reliably from eggnog Ortholog database +
+ Status bar shows 'Marked x columns + containing features of type Highlight' when 'B' is pressed + to mark columns containing highlighted regions. +
+ Dropping a PDB file onto a sequence + doesn't always add secondary structure annotation. +

- - +

- 2.10.0b1
- 25/10/2016 + 2.10.1
29/11/2016

- Application -

3D Structure chooser opens with 'Cached structures' - view if structures already loaded
Progress bar reports models as they are loaded to - structure views

- -

General

Colour by conservation always enabled and no tick - shown in menu when BLOSUM or PID shading applied
FER1_ARATH and FER2_ARATH labels were switched in - example sequences/projects/trees
+ Improved memory usage: sparse arrays used + for all consensus calculations +
+ Jmol updated to version 14.6.4 (released + 3rd Oct 2016) +
Updated Jalview's Certum code signing certificate + for 2016-2017

Application

Jalview projects with views of local PDB structure - files saved on Windows cannot be opened on OSX
Multiple structure views can be opened and - superposed without timeout for structures with multiple - models or multiple sequences in alignment
Cannot import or associated local PDB files without - a PDB ID HEADER line
RMSD is not output in Jmol console when - superposition is performed
Drag and drop of URL from Browser fails for Linux - and OSX versions earlier than El Capitan
ENA client ignores invalid content from ENA server
Exceptions are not raised in console when ENA - client attempts to fetch non-existent IDs via Fetch DB - Refs UI option
Exceptions are not raised in console when a new - view is created on the alignment
OSX right-click fixed for group selections: - CMD-click to insert/remove gaps in groups and CTRL-click - to open group pop-up menu
+ Sequence ID tool tip presents abridged + set of database cross-references, sorted alphabetically +
+ New replacement token for creating URLs just + from database cross references. Users with custom links + will receive a warning + dialog asking them to update their preferences. +
+ Cancel button and escape listener on + dialog warning user about disconnecting Jalview from a + Chimera session +
+ Jalview's Chimera control window closes if + the Chimera it is connected to is shut down +
+ New keystroke (B) and Select highlighted + columns menu item to mark columns containing highlighted + regions (e.g. from structure selections or results of a + Find operation) +
+ Command line option for batch-generation + of HTML pages rendering alignment data with the BioJS + MSAviewer +

- Build and deployment +

+ +

+ General

URL link checker now copes with multi-line anchor - tags
+ Columns with more than one modal residue + are not coloured or thresholded according to percent + identity (first observed in Jalview 2.8.2) +
+ Threonine incorrectly reported as not + hydrophobic +
+ Updates to documentation pages (above PID + threshold, amino acid properties) +
+ Lower case residues in sequences are not + reported as mapped to residues in a structure file in the + View Mapping report +
+ Identical features with non-numeric scores + could be added multiple times to a sequence +
+ Disulphide + bond features shown as two highlighted residues rather + than a range in linked structure views, and treated + correctly when selecting and computing trees from features +
+ Custom URL links for database + cross-references are matched to database name regardless + of case +

- New Known Issues + Application

Drag and drop from URL links in browsers do not - work on Windows
+ Custom URL links for specific database + names without regular expressions also offer links from + Sequence ID +
+ Removing a single configured link in the + URL links pane in Connections preferences doesn't actually + update Jalview configuration +
+ CTRL-Click on a selected region to open + the alignment area popup menu doesn't work on El-Capitan +
+ Jalview doesn't offer to associate mmCIF + files with similarly named sequences if dropped onto the + alignment +
+ Additional mappings are shown for PDB + entries where more chains exist in the PDB accession than + are reported in the SIFTS file +
+ Certain structures do not get mapped to + the structure view when displayed with Chimera +
+ No chains shown in the Chimera view + panel's View->Show Chains submenu +
+ Export as HTML with embedded SVG doesn't + work for wrapped alignment views +
+ Rename UI components for running JPred + predictions from 'JNet' to 'JPred' +
+ Export as PNG or SVG is + corrupted when annotation panel vertical scroll is not at + first annotation row +
+ Attempting to view structure for Hen + lysozyme results in a PDB Client error dialog box +
+ Structure View's mapping report switched + ranges for PDB and sequence for SIFTS +

+ +

+ 2.10.0b1
+ 25/10/2016 +

+ + Application +

3D Structure chooser opens with 'Cached structures' + view if structures already loaded
Progress bar reports models as they are loaded to + structure views

+ +

+ General +

Colour by conservation always enabled and no tick + shown in menu when BLOSUM or PID shading applied
FER1_ARATH and FER2_ARATH labels were switched in + example sequences/projects/trees

+ Application +

Jalview projects with views of local PDB structure + files saved on Windows cannot be opened on OSX
Multiple structure views can be opened and superposed + without timeout for structures with multiple models or + multiple sequences in alignment
Cannot import or associated local PDB files without a + PDB ID HEADER line
RMSD is not output in Jmol console when superposition + is performed
Drag and drop of URL from Browser fails for Linux and + OSX versions earlier than El Capitan
ENA client ignores invalid content from ENA server
Exceptions are not raised in console when ENA client + attempts to fetch non-existent IDs via Fetch DB Refs UI + option
Exceptions are not raised in console when a new view + is created on the alignment
OSX right-click fixed for group selections: CMD-click + to insert/remove gaps in groups and CTRL-click to open group + pop-up menu

+ Build and deployment +

URL link checker now copes with multi-line anchor + tags

+ New Known Issues +

Drag and drop from URL links in browsers do not work + on Windows

+ +

@@ -192,8 +1008,8 @@ General

- Updated Spanish translations. -
Jmol now primary parser for importing structure data to Jalview. Enables mmCIF and @@ -483,7 +1299,7 @@ load even when Consensus calculation is disabled
- Remove right on penultimate column of + Remove right on penultimate column of alignment does nothing

@@ -571,7 +1387,8 @@

Enable 'Get Cross-References' in menu - after fetching cross-references, and restoring from project + after fetching cross-references, and restoring from + project

Mouseover of a copy of a sequence is not @@ -636,7 +1453,8 @@ Unable to read old Jalview projects that contain non-XML data added after Jalvew wrote project.

Newly created annotation row reorders +

+ Newly created annotation row reorders after clicking on it to create new annotation for a column.

@@ -708,9 +1526,12 @@

Applet

Split frame example added to applet examples page

Build and Deployment + Build and Deployment

New ant target for running Jalview's test suite
+ New ant target for running Jalview's test + suite +

@@ -1098,10 +1919,10 @@ Deployment and Documentation +

+ General +

+ --> Deployment and Documentation

2G and 1G options in launchApp have no effect on memory allocation

Certum

Jalview SRS links replaced by UniProt and EBI-search -
Jalview SRS links replaced by UniProt and EBI-search
Output in Stockholm format
Allow import of data from gzipped files
Export/import group and sequence associated line @@ -1939,11 +2759,6 @@
- URL links generated from description line for regular-expression based URL links (applet and application) - - - - -
- Non-positional feature URL links are shown in link menu
- Enable or disable non-positional feature and database references in sequence ID tooltip from View menu in application.
- Group-associated consensus, sequence logos and conservation plots
- Symbol distributions for each column can be exported @@ -2396,11 +3211,6 @@
- Cancel button for DAS Feature Fetching
- PCA and PDB Viewers zoom via mouse roller
- User-defined sub-tree colours and sub-tree selection - - - - -
- 'New Window' button on the 'Output to Text box'
@@ -2415,11 +3225,6 @@
Fixed Remove Empty Columns Bug (empty columns at end of alignment)
Slowed DAS Feature Fetching for increased robustness. - - - - -
Made angle brackets in ASCII feature descriptions display correctly @@ -2431,11 +3236,6 @@
WsDbFetch query/result association resolved
Tree leaf to sequence mapping improved
Smooth fonts switch moved to FontChooser dialog box. - - - - -