X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=src%2Fjalview%2Fext%2Fensembl%2FEnsemblCdna.java;h=952f01e9fb57cbfddd4f0b2ac46fbdc090fa6dfe;hb=8c5cefb3ac80bd094a8dab7ce6735a11583b1772;hp=4fc80ca17412af87d13b5fadc1b1c41b46156564;hpb=9eec40eae88ae4330adc4d6ae38c513742b9e853;p=jalview.git diff --git a/src/jalview/ext/ensembl/EnsemblCdna.java b/src/jalview/ext/ensembl/EnsemblCdna.java index 4fc80ca..952f01e 100644 --- a/src/jalview/ext/ensembl/EnsemblCdna.java +++ b/src/jalview/ext/ensembl/EnsemblCdna.java @@ -1,15 +1,75 @@ +/* + * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$) + * Copyright (C) $$Year-Rel$$ The Jalview Authors + * + * This file is part of Jalview. + * + * Jalview is free software: you can redistribute it and/or + * modify it under the terms of the GNU General Public License + * as published by the Free Software Foundation, either version 3 + * of the License, or (at your option) any later version. + * + * Jalview is distributed in the hope that it will be useful, but + * WITHOUT ANY WARRANTY; without even the implied warranty + * of MERCHANTABILITY or FITNESS FOR A PARTICULAR + * PURPOSE. See the GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with Jalview. If not, see . + * The Jalview Authors are detailed in the 'AUTHORS' file. + */ package jalview.ext.ensembl; -import jalview.ext.ensembl.SeqFetcher.EnsemblSeqType; +import jalview.datamodel.SequenceFeature; +import jalview.io.gff.SequenceOntologyFactory; +import jalview.io.gff.SequenceOntologyI; +import com.stevesoft.pat.Regex; + +/** + * A client to fetch CDNA sequence from Ensembl (i.e. that part of the genomic + * sequence that is transcribed to RNA, but not necessarily translated to + * protein) + * + * @author gmcarstairs + * + */ public class EnsemblCdna extends EnsemblSeqProxy { + /* + * accepts ENST or ENSTG with 11 digits + * or ENSMUST or similar for other species + * or CCDSnnnnn.nn with at least 3 digits + */ + private static final Regex ACCESSION_REGEX = new Regex( + "(ENS([A-Z]{3}|)[TG][0-9]{11}$)" + "|" + "(CCDS[0-9.]{3,}$)"); + + /* + * fetch exon features on genomic sequence (to identify the cdna regions) + * and cds and variation features (to retain) + */ + private static final EnsemblFeatureType[] FEATURES_TO_FETCH = { + EnsemblFeatureType.exon, EnsemblFeatureType.cds, + EnsemblFeatureType.variation }; - public EnsemblCdna() throws Exception + /** + * Default constructor (to use rest.ensembl.org) + */ + public EnsemblCdna() { super(); } + /** + * Constructor given the target domain to fetch data from + * + * @param d + */ + public EnsemblCdna(String d) + { + super(d); + } + @Override public String getDbName() { @@ -22,4 +82,60 @@ public class EnsemblCdna extends EnsemblSeqProxy return EnsemblSeqType.CDNA; } + @Override + public Regex getAccessionValidator() + { + return ACCESSION_REGEX; + } + + @Override + protected EnsemblFeatureType[] getFeaturesToFetch() + { + return FEATURES_TO_FETCH; + } + + /** + * Answers true unless the feature type is 'transcript' (or a sub-type in the + * Sequence Ontology). + */ + @Override + protected boolean retainFeature(SequenceFeature sf, String accessionId) + { + if (isTranscript(sf.getType())) + { + return false; + } + return featureMayBelong(sf, accessionId); + } + + /** + * Answers true if the sequence feature type is 'exon' (or a subtype of exon + * in the Sequence Ontology), and the Parent of the feature is the transcript + * we are retrieving + */ + @Override + protected boolean identifiesSequence(SequenceFeature sf, String accId) + { + if (SequenceOntologyFactory.getInstance().isA(sf.getType(), + SequenceOntologyI.EXON)) + { + String parentFeature = (String) sf.getValue(PARENT); + if (("transcript:" + accId).equals(parentFeature)) + { + return true; + } + } + return false; + } + + /** + * Parameter object_type=Transcaript added to ensure cdna and not peptide is + * returned (JAL-2529) + */ + @Override + protected String getObjectType() + { + return OBJECT_TYPE_TRANSCRIPT; + } + }