X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=test%2Fjalview%2Fdatamodel%2FAlignmentTest.java;h=3b3d9267b77ad239498aea6ac28bb5a8e1eb8b84;hb=c9f31322ad542be7fab91e0c9f2ff3638a25c5b1;hp=89121556f1427e90f4b8ea2bd2f1ba424693c348;hpb=285b51fa6f49054040c72dfb2a64db637dc05c79;p=jalview.git diff --git a/test/jalview/datamodel/AlignmentTest.java b/test/jalview/datamodel/AlignmentTest.java index 8912155..3b3d926 100644 --- a/test/jalview/datamodel/AlignmentTest.java +++ b/test/jalview/datamodel/AlignmentTest.java @@ -60,11 +60,13 @@ public class AlignmentTest * Helper method to load an alignment and ensure dataset sequences are set up. * * @param data - * @param format TODO + * @param format + * TODO * @return * @throws IOException */ - protected AlignmentI loadAlignment(final String data, String format) throws IOException + protected AlignmentI loadAlignment(final String data, String format) + throws IOException { Alignment a = new FormatAdapter().readFile(data, AppletFormatAdapter.PASTE, format); @@ -83,8 +85,7 @@ public class AlignmentTest int i = 0; for (AlignmentAnnotation ann : al.getAlignmentAnnotation()) { - ann.setCalcId("CalcIdFor" - + al.getSequenceAt(i).getName()); + ann.setCalcId("CalcIdFor" + al.getSequenceAt(i).getName()); i++; } } @@ -104,6 +105,35 @@ public class AlignmentTest assertFalse(iter.hasNext()); } + @Test + public void testDeleteAllAnnotations_includingAutocalculated() + { + AlignmentAnnotation aa = new AlignmentAnnotation("Consensus", + "Consensus", 0.5); + aa.autoCalculated = true; + al.addAnnotation(aa); + AlignmentAnnotation[] anns = al.getAlignmentAnnotation(); + assertEquals("Wrong number of annotations before deleting", 4, + anns.length); + al.deleteAllAnnotations(true); + assertEquals("Not all deleted", 0, al.getAlignmentAnnotation().length); + } + + @Test + public void testDeleteAllAnnotations_excludingAutocalculated() + { + AlignmentAnnotation aa = new AlignmentAnnotation("Consensus", + "Consensus", 0.5); + aa.autoCalculated = true; + al.addAnnotation(aa); + AlignmentAnnotation[] anns = al.getAlignmentAnnotation(); + assertEquals("Wrong number of annotations before deleting", 4, + anns.length); + al.deleteAllAnnotations(false); + assertEquals("Not just one annotation left", 1, + al.getAlignmentAnnotation().length); + } + /** * Tests for realigning as per a supplied alignment: Dna as Dna. * @@ -133,7 +163,7 @@ public class AlignmentTest acf.addMap(al2.getSequenceAt(1), al1.getSequenceAt(1), ml); al1.addCodonFrame(acf); - al2.alignAs(al1); + ((Alignment) al2).alignAs(al1, false, true); assertEquals("GC-TC--GUC-GTA-CT", al2.getSequenceAt(0) .getSequenceAsString()); assertEquals("-GG-GTC--AGG---CAGT", al2.getSequenceAt(1) @@ -153,7 +183,7 @@ public class AlignmentTest String before0 = al2.getSequenceAt(0).getSequenceAsString(); String before1 = al2.getSequenceAt(1).getSequenceAsString(); - al2.alignAs(al1); + ((Alignment) al2).alignAs(al1, false, true); assertEquals(before0, al2.getSequenceAt(0).getSequenceAsString()); assertEquals(before1, al2.getSequenceAt(1).getSequenceAsString()); } @@ -179,10 +209,69 @@ public class AlignmentTest acf.addMap(al1.getSequenceAt(1), al2.getSequenceAt(1), ml); al2.addCodonFrame(acf); - al1.alignAs(al2); + /* + * Realign DNA; currently keeping existing gaps in introns only + */ + ((Alignment) al1).alignAs(al2, false, true); assertEquals("ACG---GCUCCA------ACT", al1.getSequenceAt(0) .getSequenceAsString()); - assertEquals("---CGT---TAACGA---AGT---", al1.getSequenceAt(1) + assertEquals("---CGT---TAACGA---AGT", al1.getSequenceAt(1) + .getSequenceAsString()); + } + + /** + * Test aligning dna as per protein alignment, for the case where there are + * introns (i.e. some dna sites have no mapping from a peptide). + * + * @throws IOException + */ + @Test + public void testAlignAs_dnaAsProtein_withIntrons() throws IOException + { + /* + * Load alignments and add mappings for cDNA to protein + */ + String dna1 = "A-Aa-gG-GCC-cT-TT"; + String dna2 = "c--CCGgg-TT--T-AA-A"; + AlignmentI al1 = loadAlignment(">Seq1\n" + dna1 + "\n>Seq2\n" + dna2 + + "\n", "FASTA"); + AlignmentI al2 = loadAlignment(">Seq1\n-P--YK\n>Seq2\nG-T--F\n", + "FASTA"); + AlignedCodonFrame acf = new AlignedCodonFrame(); + // Seq1 has intron at dna positions 3,4,9 so splice is AAG GCC TTT + // Seq2 has intron at dna positions 1,5,6 so splice is CCG TTT AAA + MapList ml1 = new MapList(new int[] + { 1, 2, 5, 8, 10, 12 }, new int[] + { 1, 3 }, 3, 1); + acf.addMap(al1.getSequenceAt(0), al2.getSequenceAt(0), ml1); + MapList ml2 = new MapList(new int[] + { 2, 4, 7, 12 }, new int[] + { 1, 3 }, 3, 1); + acf.addMap(al1.getSequenceAt(1), al2.getSequenceAt(1), ml2); + al2.addCodonFrame(acf); + + /* + * Align ignoring gaps in dna introns and exons + */ + ((Alignment) al1).alignAs(al2, false, false); + assertEquals("---AAagG------GCCcTTT", al1.getSequenceAt(0) + .getSequenceAsString()); + // note 1 gap in protein corresponds to 'gg-' in DNA (3 positions) + assertEquals("cCCGgg-TTT------AAA", al1.getSequenceAt(1) + .getSequenceAsString()); + + /* + * Reset and realign, preserving gaps in dna introns and exons + */ + al1.getSequenceAt(0).setSequence(dna1); + al1.getSequenceAt(1).setSequence(dna2); + ((Alignment) al1).alignAs(al2, true, true); + // String dna1 = "A-Aa-gG-GCC-cT-TT"; + // String dna2 = "c--CCGgg-TT--T-AA-A"; + // assumption: we include 'the greater of' protein/dna gap lengths, not both + assertEquals("---A-Aa-gG------GCC-cT-TT", al1.getSequenceAt(0) + .getSequenceAsString()); + assertEquals("c--CCGgg-TT--T------AA-A", al1.getSequenceAt(1) .getSequenceAsString()); } }