X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=wiki%2FGSDI.wiki;h=ac4db98a08c33c77f229a54db9d7c12d9bb62511;hb=78eb49e5b7c8d46b0c3a750400c9455e125d57ed;hp=c5eb5e63754e18e2eee7ad8159714ac9ae2b83c1;hpb=d89babefe5728a059761b9053f90d0adadf66dd7;p=jalview.git diff --git a/wiki/GSDI.wiki b/wiki/GSDI.wiki index c5eb5e6..ac4db98 100644 --- a/wiki/GSDI.wiki +++ b/wiki/GSDI.wiki @@ -7,34 +7,50 @@ To infer duplication events on a gene tree given a trusted species tree. == Usage == - +{{{ java -Xmx1024m -cp path/to/forester.jar org.forester.application.gsdi [-options] - +}}} === Options === * -g: to allow stripping of gene tree nodes without a matching species in the species tree * -m: use most parimonious duplication model for GSDI: assign nodes as speciations which would otherwise be assiged as potential duplications due tp polytomies in the species tree + * -q: to allow species tree in other formats than phyloXML (i.e. Newick, NHX, Nexus) + * -b: to use SDIse algorithm instead of GSDI algorithm (for binary species trees) ==== Gene tree ==== -Must be in phyloXM format, with taxonomy and sequence data in appropriate fields. +Must be in phyloXM format, with taxonomy and sequence data in appropriate fields ([http://forester.googlecode.com/files/wnt_gene_tree.xml example]). ==== Species tree ==== -Must be in phyloXML format unless option -q is used. +Must be in phyloXML format unless option -q is used ([http://forester.googlecode.com/files/species.xml example]). === Output === Besides the main output of a gene tree with duplications and speciations assigned to all of its internal nodes, this program also produces the following: - * a log file, ending in `"_gsdi_log.txt"` + * a log file, ending in `"_gsdi_log.txt"` ([http://forester.googlecode.com/files/wnt_gsdi_log.txt example]) * a species tree file which only contains external nodes with were needed for the reconciliation, ending in `"_species_tree_used.xml"` - * if the gene tree contains species with scientific species names such as "Pyrococcus horikoshii strain ATCC 700860" and if a mapping cannot be establish bases on these, GSDI will attempt to map by removing the "strain" (or "subspecies") information, these will be listed in a file ending in `"_gsdi_remapped.txt"`. + * if the gene tree contains species with scientific species names such as "Pyrococcus horikoshii strain ATCC 700860" and if a mapping cannot be establish based on these, GSDI will attempt to map by removing the "strain" (or "subspecies") information, these will be listed in a file ending in `"_gsdi_remapped.txt"`. + +=== Taxonomic mapping between gene and species tree === + +GSDI can establish a taxonomic mapping between gene and species tree on the following three data fields: + * scientific names (e.g. "Pyrococcus horikoshii") + * taxonomic identifiers (e.g. "35932" from uniprot or ncbi) + * taxonomy codes (e.g. "PYRHO") + + === Example === -`gsdi -g gene_tree.xml tree_of_life.xml out.xml` +`gsdi -g -q gene_tree.xml tree_of_life.nwk out.xml` + +=== Example files === + * [http://forester.googlecode.com/files/wnt_gene_tree.xml gene tree] + * [http://forester.googlecode.com/files/species.xml species tree] + * [http://forester.googlecode.com/files/wnt_gsdi_log.txt log file (output)] == Reference ==