X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=wiki%2FGSDI.wiki;h=ac4db98a08c33c77f229a54db9d7c12d9bb62511;hb=c3c6fec29bef1013ad55aa54516689ce22952a0a;hp=7c2f38c7ee3cfec5267addd109c527860b36e26d;hpb=37cbc3f1e61af6d52765fa176f36cca544945003;p=jalview.git diff --git a/wiki/GSDI.wiki b/wiki/GSDI.wiki index 7c2f38c..ac4db98 100644 --- a/wiki/GSDI.wiki +++ b/wiki/GSDI.wiki @@ -9,21 +9,48 @@ To infer duplication events on a gene tree given a trusted species tree. == Usage == {{{ java -Xmx1024m -cp -path/to/forester.jar org.forester.application.gsdi [-options] [outfile] +path/to/forester.jar org.forester.application.gsdi [-options] }}} - === Options === - * -s: to strip the species tree prior to duplication inference - * -b: to use SDI algorithm instead of GSDI algorithm - * -m: use most parimonious duplication model for GSDI: assign nodes as speciations which would otherwise be assiged as unknown because of polytomies in the species tree - * -q: to allow species tree in other formats than phyloXML (Newick, NHX, Nexus) + * -g: to allow stripping of gene tree nodes without a matching species in the species tree + + * -m: use most parimonious duplication model for GSDI: assign nodes as speciations which would otherwise be assiged as potential duplications due tp polytomies in the species tree + + * -q: to allow species tree in other formats than phyloXML (i.e. Newick, NHX, Nexus) -==== Species tree ==== -In phyloXML format (unless option -q is used), with taxonomy data in appropriate fields. Must be rooted, polytomies are allowed. + * -b: to use SDIse algorithm instead of GSDI algorithm (for binary species trees) ==== Gene tree ==== -In phyloXML format, with taxonomy and sequence data in appropriate fields. Must be rooted an binary (no polytomies). +Must be in phyloXM format, with taxonomy and sequence data in appropriate fields ([http://forester.googlecode.com/files/wnt_gene_tree.xml example]). + +==== Species tree ==== +Must be in phyloXML format unless option -q is used ([http://forester.googlecode.com/files/species.xml example]). + +=== Output === + +Besides the main output of a gene tree with duplications and speciations assigned to all of its internal nodes, this program also produces the following: + * a log file, ending in `"_gsdi_log.txt"` ([http://forester.googlecode.com/files/wnt_gsdi_log.txt example]) + * a species tree file which only contains external nodes with were needed for the reconciliation, ending in `"_species_tree_used.xml"` + * if the gene tree contains species with scientific species names such as "Pyrococcus horikoshii strain ATCC 700860" and if a mapping cannot be establish based on these, GSDI will attempt to map by removing the "strain" (or "subspecies") information, these will be listed in a file ending in `"_gsdi_remapped.txt"`. + +=== Taxonomic mapping between gene and species tree === + +GSDI can establish a taxonomic mapping between gene and species tree on the following three data fields: + * scientific names (e.g. "Pyrococcus horikoshii") + * taxonomic identifiers (e.g. "35932" from uniprot or ncbi) + * taxonomy codes (e.g. "PYRHO") + + + +=== Example === +`gsdi -g -q gene_tree.xml tree_of_life.nwk out.xml` + + +=== Example files === + * [http://forester.googlecode.com/files/wnt_gene_tree.xml gene tree] + * [http://forester.googlecode.com/files/species.xml species tree] + * [http://forester.googlecode.com/files/wnt_gsdi_log.txt log file (output)] == Reference ==