X-Git-Url: http://source.jalview.org/gitweb/?a=blobdiff_plain;f=wiki%2FPhyloBioRuby.wiki;h=06d577721e3e5c0ab8386b1733bf51b42297de4a;hb=211364763c73cc916843e5df5d1412d14a57359e;hp=b52eef0b88658b9c3b1715fba597936a5619ab16;hpb=26f134e7d2b98de2fd60716d67fe00d51d4fb385;p=jalview.git diff --git a/wiki/PhyloBioRuby.wiki b/wiki/PhyloBioRuby.wiki index b52eef0..06d5777 100644 --- a/wiki/PhyloBioRuby.wiki +++ b/wiki/PhyloBioRuby.wiki @@ -1,11 +1,67 @@ -#summary One-sentence summary of this page. +#summary Tutorial for multiple sequence alignments and phylogenetic methods in BioRuby -- under development! = Introduction = -Add your content here. +Tutorial for multiple sequence alignments and phylogenetic methods in BioRuby -- under development!. + + += Multiple Sequence Alignments = + +== Reading in a Multiple Sequence Alignment from a File == + +... to be done + +== Calculating a Multiple Sequence Alignment == + +BioRuby can be used to execute a variety of multiple sequence alignment +programs (such as MAFFT, Probcons, ClustalW, Muscle). +In the following, examples for using the MAFFT and + +=== MAFFT === + + + +{{{ +#!/usr/bin/env ruby +require 'bio' + +# Calculates the alignment using the MAFFT program on the local +# machine with options '--maxiterate 1000 --localpair' +# and stores the result in 'report'. +options = [ '--maxiterate', '1000', '--localpair' ] +mafft = Bio::MAFFT.new('path/to/mafft', options ) +report = mafft.query_align( seqs) + +# Accesses the actual alignment +align = report.alignment + +# Prints each sequence to the console. +report.align.each { |s| puts s.to_s } +# +}}} + +=== Muscle === + +{{{ +#!/usr/bin/env ruby +require 'bio' + +# Calculates the alignment using the Muscle program on the local +# machine with options '-quiet -maxiters 64' +# and stores the result in 'report'. +options = [ '-quiet', '-maxiters', '64' ] +muscle = Bio::Muscle.new('path/to/muscle', options ) +report = muscle.query_align( seqs) + +# Accesses the actual alignment +align = report.alignment + +# Prints each sequence to the console. +report.align.each { |s| puts s.to_s } +# +}}} -= Details = Add your content here. Format your content with: * Text in *bold* or _italic_