\exstep{ Select FER1\_SPIOL, FER1\_ARATH, FER2\_ARATH, Q93Z60\_ARATH and
O80429\_MAIZE
-(Hint: you can do this by pressing [CTRL]-I to invert the sequence selection and then
+
+{\sl Hint: you can do this by pressing [CTRL]-I to invert the sequence selection and then
deselect FER1\_MAIZE), and use the $\Rightarrow$ key to slide them to so they
-begin at column 5 of the alignment view.}
+begin at column 5 of the alignment view.}}
\exstep{ Select all the visible
sequences (those not hidden) in the block by pressing [CTRL]-A.
\exercise{Annotating Alignments}{
+ \label{annotatingalignex}
\exstep{Load the alignment at \textsf{http://www.jalview.org/tutorial/alignment.fa}.
Right-click on the {\sl Conservation} annotation row to
bring up the context menu and select {\sl Add New Row}. A dialog box will
row. Save the file and drag it onto the alignment view.}
\exstep{Add an additional helix somewhere along the row by editing the file and
re-importing it.
+
{\sl Hint: Use the Export Annotation function to view what helix annotation looks like in
a Jalview annotation file.}}
\exstep{Use the {\sl Alignment Window $\Rightarrow$ File $\Rightarrow$ Export Annotations...}
\exstep{Open the exported annotation in a text editor, and use the Annotation File Format
documentation to modify the style of the Conservation, Consensus and Quality annotation rows so
they appear as several lines on a single line graph.
+
{\sl Hint: You need to change the style of annotation row in the first field of the annotation
row entry in the file, and create an annotation row grouping to overlay the three quantitative
annotation rows.}
}
\label{viewannotfileex}
-{\sl Homework for after you have completed exercise \ref{secstrpredex}:}
+ {\sl Homework for after you have completed exercise \ref{secstrpredex}:}
+
Recover or recreate the secondary structure prediction that you made in exercise \ref{secstrpredex}. Use the {\sl File $\Rightarrow$ Export
-Annotation} function to view the Jnet secondary structure prediction annotation row. Note the
+ Annotation} function to view the Jnet secondary structure prediction annotation row.
+
+Note the
SEQUENCE\_REF statements surrounding the row specifying the sequence association for the
annotation. }
\exercise{Secondary Structure Prediction}{
\label{secstrpredex}
+
+{\sl Note: The annotation panel can get quite busy during this exercise. Try
+hiding some annotations rows by right clicking
+the mouse in the annotation label panel and select the ``Hide this row'' option.
+The Annotations dropdown menu on the alignment wndow also provides options for
+reording and hiding autocalculated and sequence associated annotation. }
+
\exstep{ Open the alignment at \url{http://www.jalview.org/tutorial/alignment.fa}. Select the sequence {\sl FER\_MESCR} by
clicking on the sequence ID. Then select {\sl Web Service $\Rightarrow$ Secondary Structure Prediction $\Rightarrow$ JNet Secondary Structure Prediction}
from the alignment window menu. A status window will appear and after some time (about 2-4 min) a new window with the JPred prediction will appear.
{\bf All} the JNet predictions for the sequences will now be visible in the
original alignment window.}
-{\sl Note: The annotation panel can get quite busy and it may be
-helpful to hide some of the annotations rows, by right clicking the mouse in
-the annotation label panel and select ``Hide this row'' option in the context
-menu}. }
+{\bf Homework:} Go back to the last step of exercise \ref{annotatingalignex} and
+follow the instructions to view the Jalview annotations file created from the annotations
+generated by the JPred server for your sequence.
+
+}
+
\begin{figure}[htbp]
\begin{center}
IDs} to retrieve all the PDB structures for the sequences.}
\exstep{Open and align
-the structures for all sequences. ({\sl Hint: see \ref{viewAllStructures} to see
+the structures for all sequences. (
+
+{\sl Hint: see \ref{viewAllStructures} to see
how to do this.})}
\exstep{Compare the disorder predictions to the structure data by mapping any