From: gmungoc Date: Sat, 17 Jan 2015 06:51:37 +0000 (+0000) Subject: JAL-1619 further refactoring X-Git-Url: http://source.jalview.org/gitweb/?a=commitdiff_plain;h=316032bb49f563f07b3cf23f9d97bfceeda21961;p=jalview.git JAL-1619 further refactoring --- diff --git a/src/jalview/analysis/Dna.java b/src/jalview/analysis/Dna.java index cf6f83e..0e7d736 100644 --- a/src/jalview/analysis/Dna.java +++ b/src/jalview/analysis/Dna.java @@ -27,6 +27,7 @@ import jalview.datamodel.AlignmentAnnotation; import jalview.datamodel.AlignmentI; import jalview.datamodel.Annotation; import jalview.datamodel.DBRefEntry; +import jalview.datamodel.DBRefSource; import jalview.datamodel.FeatureProperties; import jalview.datamodel.GraphLine; import jalview.datamodel.Mapping; @@ -152,7 +153,8 @@ public class Dna for (s = 0; s < sSize; s++) { SequenceI newseq = translateCodingRegion(cdnaseqs.get(s), - seqstring[s], viscontigs, acf, alignedCodons, gapCharacter, + seqstring[s], viscontigs, acf, alignedCodons, pepseqs, + gapCharacter, false); if (newseq != null) @@ -378,6 +380,7 @@ public class Dna * @param acf * Definition of global ORF alignment reference frame * @param alignedCodons + * @param proteinSeqs * @param gapCharacter * @param starForStop * when true stop codons will translate as '*', otherwise as 'X' @@ -385,7 +388,8 @@ public class Dna */ protected static SequenceI translateCodingRegion(SequenceI selection, String seqstring, int[] viscontigs, AlignedCodonFrame acf, - int[][] alignedCodons, char gapCharacter, + int[][] alignedCodons, List proteinSeqs, + char gapCharacter, final boolean starForStop) { List skip = new ArrayList(); @@ -552,13 +556,12 @@ public class Dna final int compareCodonPos = Dna.compareCodonPos(cdp, alignedCodons[aspos]); // debug - // System.out.println(seq + "/" + aa + " codons: " - // + Arrays.deepToString(alignedCodons)); - // System.out - // .println(("Compare " + Arrays.toString(cdp) + " at pos " - // + aspos + " with " - // + Arrays.toString(alignedCodons[aspos]) + " got " + - // compareCodonPos)); + System.out.println(seq + "/" + aa + " codons: " + + Arrays.deepToString(alignedCodons)); + System.out + .println(("Compare " + Arrays.toString(cdp) + " at pos " + + aspos + " with " + + Arrays.toString(alignedCodons[aspos]) + " got " + compareCodonPos)); // end debug switch (compareCodonPos) { @@ -568,7 +571,7 @@ public class Dna * This codon should precede the mapped positions - need to insert a * gap in all prior sequences. */ - acf.insertAAGap(aspos, gapCharacter); + insertAAGap(aspos, gapCharacter, alignedCodons, proteinSeqs); findpos = false; break; @@ -608,6 +611,7 @@ public class Dna // update maximum alignment width // (we can do this without calling checkCodonFrameWidth because it was // already done above) + System.out.println("aspos " + aspos + " >= " + acf.aaWidth); acf.setAaWidth(aspos); } // ready for next translated reading frame alignment position (if any) @@ -699,11 +703,17 @@ public class Dna { 1, resSize }, 3, 1); transferCodedFeatures(selection, newseq, map, null, null); - SequenceI rseq = newseq.deriveSequence(); // construct a dataset - // sequence for our new - // peptide, regardless. - // store a mapping (this actually stores a mapping between the dataset - // sequences for the two sequences + + /* + * Construct a dataset sequence for our new peptide. + */ + SequenceI rseq = newseq.deriveSequence(); + + /* + * Store a mapping (between the dataset sequences for the two + * sequences). + */ + // SIDE-EFFECT: acf stores the aligned sequence reseq; to remove! acf.addMap(selection, rseq, map); return rseq; } @@ -714,6 +724,33 @@ public class Dna } /** + * Insert a gap into the aligned proteins and the codon mapping array. + * + * @param pos + * @param gapCharacter + * @param alignedCodons + * @param proteinSeqs + */ + protected static void insertAAGap(int pos, char gapCharacter, + int[][] alignedCodons, List proteinSeqs) + { + System.out.println("insertAAGap " + pos + "/" + proteinSeqs.size()); + // aaWidth++; + for (SequenceI seq : proteinSeqs) + { + seq.insertCharAt(pos, gapCharacter); + } + + // if (pos < aaWidth) + // { + // aaWidth++; + System.arraycopy(alignedCodons, pos, alignedCodons, pos + 1, + alignedCodons.length - pos - 1); + alignedCodons[pos] = null; // clear so new codon position can be marked. + // } + } + + /** * Check the codons array is big enough to accommodate the given position, if * not resize it. * @@ -760,9 +797,8 @@ public class Dna SequenceFeature[] sf = (dna.getDatasetSequence() != null ? dna .getDatasetSequence() : dna).getSequenceFeatures(); Boolean fgstate; - jalview.datamodel.DBRefEntry[] dnarefs = jalview.util.DBRefUtils - .selectRefs(dna.getDBRef(), - jalview.datamodel.DBRefSource.DNACODINGDBS); + DBRefEntry[] dnarefs = DBRefUtils.selectRefs(dna.getDBRef(), + DBRefSource.DNACODINGDBS); if (dnarefs != null) { // intersect with pep