From: gmungoc
Sequence Name Italics - select to apply the italicised -vbersion of the font to sequence labels.
+version of the font to sequence labels.Smooth Font - Toggles anti-aliasing on / off for faster rendering of the alignment.
Wrap Alignment - Select whether to open new alignment windows in wrapped mode or not.
Gap Symbol - The default gap symbol may be set to either "-" or "."
-Sort by - When the alignment is loaded in, it will can -be sorted by Id or pairwise identity.
+Sort alignment by - When the alignment is loaded in, it can +be ordered as read (No sort), or sorted by Id or pairwise identity.
+Sort annotations by - Annotations can be unsorted, sorted by the order of the related sequences in +the alignment, or by label. Autocalculated annotations (e.g. Consensus) can be shown either last (below sequence +annotations) or first (above sequence annotations). Since Jalview 2.8.2.
Open file - If this is selected then the default alignment file will be opened when Jalview is started. You can change the default file by clicking on file name and either typing in the file