From: jprocter Alignment Annotation In addition to the definition of groups and sequence features, Jalview also
- allows you to mark particular columns of an alignment and add symbols and text
+ In addition to the definition of groups and sequence features,
+ Jalview can display symbols and graphs under the columns of an
+ alignment, and allow you to mark particular columns of an alignment and add symbols and text
in the annotation area shown below the alignment (which may be hidden if View→Show
Annotation is not ticked). As of Jalview 2.08 precalculated annotations can be added to alignments from
- the command line, drag and drop, or from the "File-> Load Features /
- Annotations" menu item. See the Annotations
- File Format for more details. Web services can also add annotation to an alignment (see the
+ JNet web service), and as of Jalview 2.08 quantitative and symbolic
+ annotations can be added to an alignment via an Annotations
+ File for more details.
Annotation rows are added using the Annotation Label
@@ -68,11 +69,8 @@ the row. Note: This cannot be undone
-Annotation is stored and retrieved using Jalview Archives. Quantitative
-annnotation is also possible, but currently only possible with the
-JNet web service, or by creating your own Jalview Archive (which
-contains XML conforming to the Jalview-Vamsas XML schema).
+User defined annotation is stored and retrieved using Jalview Archives.
Current Limitations
Sequence associations
are turned off for subsequent annotation definitions by:
SEQUENCE_REF ALIGNMENT
LINE_GRAPH type annotations can be given a colour,
- combined onto the same vertical axis, and have abscissa (lines of
- constant value - so they are horizontal) at particular
- values using the following lines (respectively):
-COLOUR graph name colour
-COMBINE graph 1 name graph 2 name
-GRAPHLINE graph name value label colour
An example Annotation file is given below:
-#Comment lines follow the hash symbol
- JALVIEW_ANNOTATION
- SEQUENCE_REF FER1_MESCR 5
- BAR_GRAPH Bar Graph 1 ||-100,-|-200,-|-300,-|-400,-|200,+|300,+|150,+
- LINE_GRAPH Green Values 1.1|2.2|1.3|3.4|0.7|1.4|3.3|2.2|2.1|-1.1|3.2
- LINE_GRAPH Red Values 2.1|3.2|1.3|-1.4|5.5|1.4|1.3|4.2|-1.1|1.1|3.2
- BAR_GRAPH Bar Graph 2 1,.|2,*|3,:|4,.|5,*|4,:|3,.|2|1|1|2|3|4|5|4
- NO_GRAPH Icons ||||E,Sheet1|E|E||||H,Sheet 2|H|H|H||||||
- NO_GRAPH Purple Letters m|y|p|r|o|t|e|i|n
COLOUR Bar Graph 2 blue
- COLOUR Red Values 255,0,0
- COLOUR Green Values green
- COLOUR Purple Letters 151,52,228
- COMBINE Green Values Red Values
GRAPHLINE Red Values 2.6 threshold black
-
-
-
LINE_GRAPH type annotations can be given a colour
+ (specified as 24 bit RGB triplet in hexadecimal or comma separated values),
+ combined onto the same vertical axis, and have ordinate lines (horizontal
+ lines at a particular vertical axis value) using the following
+ commands (respectively):
+COLOUR graph_name colour
+COMBINE graph_1_name graph_2_name
+GRAPHLINE graph_name value label colour
+
An example Annotation file is given below: +
#Comment lines follow the hash symbol +JALVIEW_ANNOTATION +SEQUENCE_REF FER1_MESCR 5 +BAR_GRAPH Bar Graph 1 ||-100,-|-200,-|-300,-|-400,-|200,+|300,+|150,+ +LINE_GRAPH Green Values 1.1|2.2|1.3|3.4|0.7|1.4|3.3|2.2|2.1|-1.1|3.2 +LINE_GRAPH Red Values 2.1|3.2|1.3|-1.4|5.5|1.4|1.3|4.2|-1.1|1.1|3.2 +BAR_GRAPH Bar Graph 2 1,.|2,*|3,:|4,.|5,*|4,:|3,.|2|1|1|2|3|4|5|4 +NO_GRAPH Icons ||||E,Sheet1|E|E||||H,Sheet 2|H|H|H|||||| +NO_GRAPH Purple Letters m|y|p|r|o|t|e|i|n +COLOUR Bar Graph 2 blue +COLOUR Red Values 255,0,0 +COLOUR Green Values green +COLOUR Purple Letters 151,52,228 +COMBINE Green Values Red Values +GRAPHLINE Red Values 2.6 threshold black +