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z?`icVh9yuY*P}DAj;2>bLy>JIY5VZ1ru4(PyU~{9cSXrUQ?2LKC=^~b@buscyAa3{h_+=htaEbV>FtIFm!z8T`+xjk5?%YMoCMG@xEUgT| z>*=NQfd0wPfPR%o^{bHh@8CCGRq-dV!0AOP*eK%<25v_p%kuF*k$i>Vf08$RTAUCJ zmmDJRza4}b3u$cDWB4gyt)Te)D+__y>TbJc`82ysEN|`Fio~EY?ZnLY4b>aRXtG$w zK#TMzkmmiF2ST7}|>Q2BXQcWTv%4R9st`IS4O6$fp5z!S7W;x#w#%NWA`{q#~ zNKf#gP${z}ROhgjo42xoTmOHv*7k45MgR0hmGK{UME_WVQ~kH&6#t2jl@kAER*-bO zu#GkQXi!;;GZX6o`>GQ$3*tlVMj=eXBan#aAI5Jk`@dk4{kLX3g5|$HpgLKL$hrnh zS%a@9KTpX87Y%|Lj1$2tSCE_M`dbS}#D^TRu3O8SjYRownyT+VN`n~X + +Annotation Colouring + + +

Annotation Colouring

+

Jalview 2.08 allows an alignment to be coloured on a per-column basis based + on any numerical annotation added to that alignment.

+Select "Colour" ".. + by Annotation" to bring up the Colour by Annotation settings window.
+
+
+
    +
  • Select which annotation to base the colouring scheme on using the top left + selection box.
  • +
  • If the "Use Original Colours" box is selected, the colouring + scheme will use the colouring scheme present on the alignment before the Annotation + Colour Settings window was displayed.
  • +
  • The colour scheme can display a colour gradient from a colour representing + the minimum value in the selected annotation to a colour representing the + maximum value in the selected annotation. Use the "Min Colour" and + "Max Colour" to set the colour gradient range.
  • +
  • Select whether to colour the alignment above or below an adjustable threshold + with the selection box center left of the window.
  • +
  • Change the threshold value with the slider, or enter the exact value in + the text box.
  • +
+

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+Annotations File Format + + +

Alignment Annotations File Format
+

+ A precalculated annotations fiile can read onto an alignment from the command + line ("-annotations"), by drag and dropping the the annotations file + onto an alignment or by selecting from the File menu "Load Features / Annotations".

+

The File is in tab delimited format. The file must have the line JALVIEW_ANNOTATION + as an identifier. Then a block of annotations are added in the form GRAPH_TYPE + Label Values

+

GRAPH_TYPE can be either BAR_GRAPH, LINE_GRAPH or NO_GRAPH. The values are + per alignment column, separated by "|". Multiple content per column + can be separated with commas, Jalview will display the content if it interprates + the content as a text label, or secondary structure character (H or E)

+

You can optionally associate an annotation with a sequence by adding a line + SEQUENCE_REFseq_namestartIndex All Annotations after a SEQUENCE_REF will be + associated with that sequence. Use SEQUENCE_REF ALIGNMENT to cancel the associtations.

+

The visual graphs can be coloured or combined with other graphs, or have an + arbitrary line drawn at a certain value using the following lines.

+

COLOUR<tab>graph name<tab>colour
+ COMBINE<tab>graph 1 name<tab>graph 2 name
+ GRAPHLINE<tab>graph name<tab>value<tab>label<tab>colour

+

An example Annotation file may look like this:

+

#Comment lines follow the hash symbol
+ JALVIEW_ANNOTATION
+ SEQUENCE_REF FER1_MESCR 5
+ BAR_GRAPH<tab>Bar Graph 1<tab>||-100,-|-200,-|-300,-|-400,-|200,+|300,+|150,+
+ LINE_GRAPH<tab>Green Values<tab>1.1|2.2|1.3|3.4|0.7|1.4|3.3|2.2|2.1|-1.1|3.2
+ LINE_GRAPH<tab>Red Values<tab>2.1|3.2|1.3|-1.4|5.5|1.4|1.3|4.2|-1.1|1.1|3.2
+ BAR_GRAPH<tab>Bar Graph<tab>2 1,.|2,*|3,:|4,.|5,*|4,:|3,.|2|1|1|2|3|4|5|4
+ NO_GRAPH<tab>Icons <tab>||||E,Sheet1|E|E||||H,Sheet 2|H|H|H||||||
+ NO_GRAPH<tab>Purple Letters<tab>m|y|p|r|o|t|e|i|n

+

COLOUR<tab>Bar Graph 2<tab>blue
+ COLOUR<tab>Red Values<tab>255,0,0
+ COLOUR<tab>Green Values<tab>green
+ COLOUR<tab>Purple Letters<tab>151,52,228
+ COMBINE<tab>Green Values<tab>Red Values

+

GRAPHLINE<tab>Red Values<tab>2.6<tab>threshold<tab>black +
+

+


+

+ + diff --git a/help/html/features/featuresFormat.html b/help/html/features/featuresFormat.html new file mode 100755 index 0000000..8e6ee13 --- /dev/null +++ b/help/html/features/featuresFormat.html @@ -0,0 +1,35 @@ + + +Features File Format + + +

Sequence Features File Format

+

(Prior to version 2.08 known as the "Groups file")
+ A precalculated Features file can read onto an alignment from the command line + ("-features"), by drag and dropping the features file onto an alignment + or by selecting from the File menu "Load Features / Annotations".

+

Specify the feature types first, then refer to the feature type for each sequence.

+

featureType<tab>colour
+ description<tab>sequenceId<tab>sequenceIndex<tab>start<tab>end<tab>featureType

+

eg
+ domain red
+ metal ion-binding site 00ff00
+ transit peptide 0,105,215
+ chain 225,105,0
+ modified residue 105,225,35
+ signal peptide 0,155,165
+ Your Own description here FER_CAPAA -1 3 93 domain
+ Your Own description here FER_CAPAN -1 48 144 chain
+ Your Own description here FER_CAPAN -1 50 140 domain
+ Your Own description here FER_CAPAN -1 136 136 modified residue
+ Your Own description here FER1_LYCES -1 1 47 transit peptide
+ Your Own description here Q93XJ9_SOLTU -1 1 48 signal peptide
+ Your Own description here Q93XJ9_SOLTU -1 49 144 chain

+

An additional option in Jalview 2.08 is to group features in the following + way:

+

STARTGROUP<tab>My feature groupA
+ ....Many Feature descriptions here
+ ENDGROUP<tab>My feature groupA

+

+ + -- 1.7.10.2