From 13d88f802c99cc65aafd903e6a7bada5e64a1525 Mon Sep 17 00:00:00 2001 From: Jim Procter Date: Tue, 11 Oct 2016 11:36:18 +0000 Subject: [PATCH] hints should always be placed on a new line in the exercise. also add link between the annotation and jpred exercises for the 'homework' --- TheJalviewTutorial.tex | 36 +++++++++++++++++++++++++++--------- 1 file changed, 27 insertions(+), 9 deletions(-) diff --git a/TheJalviewTutorial.tex b/TheJalviewTutorial.tex index 751643c..e677945 100644 --- a/TheJalviewTutorial.tex +++ b/TheJalviewTutorial.tex @@ -1217,9 +1217,10 @@ key.} \exstep{ Select FER1\_SPIOL, FER1\_ARATH, FER2\_ARATH, Q93Z60\_ARATH and O80429\_MAIZE -(Hint: you can do this by pressing [CTRL]-I to invert the sequence selection and then + +{\sl Hint: you can do this by pressing [CTRL]-I to invert the sequence selection and then deselect FER1\_MAIZE), and use the $\Rightarrow$ key to slide them to so they -begin at column 5 of the alignment view.} +begin at column 5 of the alignment view.}} \exstep{ Select all the visible sequences (those not hidden) in the block by pressing [CTRL]-A. @@ -1867,6 +1868,7 @@ calculations can be found in the on-line documentation. \exercise{Annotating Alignments}{ + \label{annotatingalignex} \exstep{Load the alignment at \textsf{http://www.jalview.org/tutorial/alignment.fa}. Right-click on the {\sl Conservation} annotation row to bring up the context menu and select {\sl Add New Row}. A dialog box will @@ -1905,6 +1907,7 @@ pane. } row. Save the file and drag it onto the alignment view.} \exstep{Add an additional helix somewhere along the row by editing the file and re-importing it. + {\sl Hint: Use the Export Annotation function to view what helix annotation looks like in a Jalview annotation file.}} \exstep{Use the {\sl Alignment Window $\Rightarrow$ File $\Rightarrow$ Export Annotations...} @@ -1912,14 +1915,18 @@ function to export all the alignment's annotation to a file.} \exstep{Open the exported annotation in a text editor, and use the Annotation File Format documentation to modify the style of the Conservation, Consensus and Quality annotation rows so they appear as several lines on a single line graph. + {\sl Hint: You need to change the style of annotation row in the first field of the annotation row entry in the file, and create an annotation row grouping to overlay the three quantitative annotation rows.} } \label{viewannotfileex} -{\sl Homework for after you have completed exercise \ref{secstrpredex}:} + {\sl Homework for after you have completed exercise \ref{secstrpredex}:} + Recover or recreate the secondary structure prediction that you made in exercise \ref{secstrpredex}. Use the {\sl File $\Rightarrow$ Export -Annotation} function to view the Jnet secondary structure prediction annotation row. Note the + Annotation} function to view the Jnet secondary structure prediction annotation row. + +Note the SEQUENCE\_REF statements surrounding the row specifying the sequence association for the annotation. } @@ -3431,6 +3438,13 @@ highlight differences in disorder prediction across aligned sequences. \exercise{Secondary Structure Prediction}{ \label{secstrpredex} + +{\sl Note: The annotation panel can get quite busy during this exercise. Try +hiding some annotations rows by right clicking +the mouse in the annotation label panel and select the ``Hide this row'' option. +The Annotations dropdown menu on the alignment wndow also provides options for +reording and hiding autocalculated and sequence associated annotation. } + \exstep{ Open the alignment at \url{http://www.jalview.org/tutorial/alignment.fa}. Select the sequence {\sl FER\_MESCR} by clicking on the sequence ID. Then select {\sl Web Service $\Rightarrow$ Secondary Structure Prediction $\Rightarrow$ JNet Secondary Structure Prediction} from the alignment window menu. A status window will appear and after some time (about 2-4 min) a new window with the JPred prediction will appear. @@ -3469,10 +3483,12 @@ Reference Annotation} option. {\bf All} the JNet predictions for the sequences will now be visible in the original alignment window.} -{\sl Note: The annotation panel can get quite busy and it may be -helpful to hide some of the annotations rows, by right clicking the mouse in -the annotation label panel and select ``Hide this row'' option in the context -menu}. } +{\bf Homework:} Go back to the last step of exercise \ref{annotatingalignex} and +follow the instructions to view the Jalview annotations file created from the annotations +generated by the JPred server for your sequence. + +} + \begin{figure}[htbp] \begin{center} @@ -3612,7 +3628,9 @@ $\Rightarrow$ Disorder Prediction } submenu.} IDs} to retrieve all the PDB structures for the sequences.} \exstep{Open and align -the structures for all sequences. ({\sl Hint: see \ref{viewAllStructures} to see +the structures for all sequences. ( + +{\sl Hint: see \ref{viewAllStructures} to see how to do this.})} \exstep{Compare the disorder predictions to the structure data by mapping any -- 1.7.10.2