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sources/alscript/src/version.h diff --git a/sources/alscript/LICENSE b/sources/alscript/LICENSE new file mode 100644 index 0000000..30b663f --- /dev/null +++ b/sources/alscript/LICENSE @@ -0,0 +1,121 @@ +SOFTWARE LICENCE AGREEMENT FOR ACADEMIC USE OF ALSCRIPT + +IMPORTANT: This software and its associated documentation are the copyright +works of the author, Geoffrey J. Barton hereinafter referred to as the +LICENSOR. Use of the software and documentation is governed by the terms of +the Academic License Agreement set out below. You, hereinafter referred to +as the LICENSEE, will not be able to install the software unless you first +agree to those terms. + +The LICENSOR has developed a body of computer software and associated +documentation called ALSCRIPT hereafter referred to as the WORK. + +The LICENSEE desires to use the WORK for education and research purposes. + +The LICENSEE and LICENSOR agree as follows: + +The LICENSOR grants to the LICENSEE a nonexclusive, nontransferable, +licence to use the WORK subject to the following conditions. + +1. LICENSEE'S RIGHTS + +The Licensee shall have the right to use the WORK for educational and +research purposes on all computers owned or leased by the Licensee and +located on the LICENSEE'S campus or site. + +2. RESTRICTIONS ON USE + +No commercial use of any kind is permitted under this licence. For +commercial use, a commercial licence is required. + +3. LICENSING FEE + +For a distribution via electronic means, there is no fee. For a physical +distribution on magnetic media, the fee is fifty (50) pounds (UK). + +4. NO SUPPORT + +The LICENSEE recognizes that the LICENSOR is not obligated to provide +support, maintenance, consulting, or revision of the WORK. If the LICENSOR +chooses to release to the LICENSEE updates of, additions to, or +modifications of the WORK, this agreement shall apply to them as though +they were part of the original WORK. + +5. NO PRODUCT WARRANTY + +The WORK is released on a "as is" basis. There is no warranty whatsoever as +to functioning, performance or effect on hardware or other software, +express or implied. The LICENSOR disclaims any implied warranties of +merchantability or fitness for any particular purpose. + +6. OWNERSHIP + +The LICENSEE agrees that the WORK including any updates, additions, and +modifications, is, and shall at all times remain, the property of the +LICENSOR, and that it has been copyrighted by the LICENSOR. The LICENSEE +shall have no right, title or interest therein or thereto except as +expressly set forth in this agreement. + +7. CREDITS + +All credits and copyright notices in the WORK, both in listings and/or +documentation, whether names of individuals or organisations, shall be +retained in place. Publications referring to the WORK, or to other works +containing the WORK in whole or in part, shall refer to it as ALSCRIPT and +shall specify that the WORK was made by Geoffrey J. Barton. Publication of +results that use the WORK shall cite: Barton, G. J. (1993), Prot. Eng., 6, +37-40. + +8. NONDISCLOSURE + +Under no conditions shall the LICENSEE disclose the WORK, in whole or in +part, to third parties, except as expressly provided for in this agreement. +Nor shall the LICENSEE make the WORK available to third parties via a +computer network. Permission is hereby granted to the LICENSEE to disclose +the WORK or modifications thereof to other organisations in possession of a +valid source licence for the WORK, provided that such disclosure shall be +for educational or research purposes only. LICENSEE may also disclose the +WORK to its students and employees for use in their educational and +research activities, provided that they are bound not to further disclose +it to third parties. This article shall survive termination of the +agreement. + +9. NO LIABILITY + +Neither the LICENSOR nor any individual or any legal entity involved in +creating, modifying, updating, or supplementing the work, shall be liable +for damages arising out of the failure or malfunctioning of the WORK. The +LICENSEE hereby assumes the risk of and releases and forever discharges the +LICENSOR, its employees and any other individual or legal entity referred +to in the foregoing sentence with respect to any expense, claim, liability, +loss or damage, direct or indirect, including any incidental or +consequential damages, whether made or suffered by LICENSEE in connection +with the failure or malfunction of the WORK. LICENSEE acknowledges that the +WORK is in the process of development and is not error-free, that the +foregoing exclusion of liability is therefore an essential term of this +Agreement without which exclusion the LICENSOR would not be willing to +enter into this Agreement and to make the Work available on the Price +agreed upon herein. + +10. GOVERNING LAW + +This agreement shall be construed and enforced according to the laws of +England. + +11. TERMINATION AND ENFORCEMENT COSTS + +LICENSOR shall have the right to terminate this agreement with immediate +effect upon notice by registered mail to LICENSEE in the event that +LICENSEE, its employees, or persons acting on its behalf breach any +provision of this agreement. Upon termination, the LICENSEE agrees to +return the orginal WORK immediately, to destroy all copies of the WORK +(exact or modified) in its possession or under its control, and to send to +the LICENSOR a signed statement that all such copies have been destroyed. +If the LICENSOR takes legal action against the LICENSEE to enforce this +agreement and prevails, the LICENSEE agrees to pay LICENSOR'S legal costs, +including reasonable attorney's fees. + +12. CHANGES TO THIS AGREEMENT + +This agreement may only be changed if both parties agree to the proposed +changes in writing. diff --git a/sources/alscript/README b/sources/alscript/README new file mode 100644 index 0000000..dc430b6 --- /dev/null +++ b/sources/alscript/README @@ -0,0 +1,6 @@ + + +This file contains details of the terms of use for alscript. + +Please see the file LICENSE for details of the Academic Licence for ALSCRIPT. + diff --git a/sources/alscript/README_WINDOWS b/sources/alscript/README_WINDOWS new file mode 100644 index 0000000..f60e266 --- /dev/null +++ b/sources/alscript/README_WINDOWS @@ -0,0 +1,20 @@ + + + +Running the alscript suite under Microsoft Windows 95/NT and Win32. + +In version 2.07 there is directory called: + +bin/cygwin + +This includes executables and a .dll file compiled to run on microsoft windows. +If you add this directory to your path, or execute alscript from this directory, +you will be able to run alscript under windows. + +This new feature uses the cygwin environment, for further details see: + +http://www.cygnus.com + +GJB 9/July/1999 + + diff --git a/sources/alscript/RELEASE_NOTES b/sources/alscript/RELEASE_NOTES new file mode 100644 index 0000000..d5e1d17 --- /dev/null +++ b/sources/alscript/RELEASE_NOTES @@ -0,0 +1,21 @@ + +Version 2.07 - 9 July 1999 + +Minor changes to makefiles to allow compilation of all modules under Linux. Addition +of cygwin .dll file to allow the code to run under windows. + +Please see the README_WINDOWS file. + +Version 2.06 + +Includes options by Morten Kjeldgaard + +small arrow up filled/hollow (@fsuparrow/@suparrow) +small arrow down filled/hollow (@fsdownarrow/@sdownarrow) +diamond filled/hollow (@fdiamond/@diamond) +bar filled/hollow (@fbar/@bar) + +Thanks to Morten for making these additions available. + + + diff --git a/sources/alscript/build b/sources/alscript/build new file mode 100755 index 0000000..7b73dfc --- /dev/null +++ b/sources/alscript/build @@ -0,0 +1,54 @@ +#!/bin/csh +# csh script to compile the elements of alscript +# +# type ./build +# +# e.g. ./build sun or ./build sgi or ./build gcc +# +# ./build linux +# +# Note that BUILD linux should also work under cygwin, though +# you will need to add --unix to the make arguments. +# In release 2.07 the cygwin binaries are included in the distribution. +# +# This script will create a subdirectory /bin if it does not +# already exist. It will then create a subsub directory /bin/machine +# where executables for the architecture will be stored. +# +# You should then add this directory to your path, or add links +# from a directory that is on your path (e.g. /usr/local/bin) +# +echo "" +echo "Trying to build alscript for $1" +echo "" +if(! -e bin) then + echo "Creating bin directory" + mkdir bin +endif +if(! -e bin/$1) then + echo "Creating subdirectory bin/$1" + mkdir bin/$1 +endif +if(-e src/alscript.o) then + echo "Deleting old object files" + /bin/rm src/*.o +endif +echo "Copying Makefile" +/bin/cp src/makefile.$1 src/makefile +echo "Attempting make" +make -C src +echo "" +echo "Moving executables to bin directory" +echo "" +/bin/mv src/alscript bin/$1 +/bin/mv src/msf2blc bin/$1 +/bin/mv src/clus2blc bin/$1 +/bin/mv src/fast2blc bin/$1 +/bin/mv src/alsnum bin/$1 +echo "" +echo "Deleting object files" +echo "" +/bin/rm src/*.o gjutil/*.o +echo "" +echo " All should be complete!" +echo "" diff --git a/sources/alscript/doc/README b/sources/alscript/doc/README new file mode 100644 index 0000000..77beb72 --- /dev/null +++ b/sources/alscript/doc/README @@ -0,0 +1,9 @@ +This directory contains documentation for ALSCRIPT. + +alscript.tex - LaTeX document should be readable in any text editor. +alscript.ps - PostScript document - should be printable on a PostScript + printer. 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+y(12,)h(387-395)164 2456 y(6.)g(Livingstone,)e(C.)i(D.)g(and)f(Barton,) +g(G.)h(J.)f(\(1993\),)949 2607 y Fh(32)p eop +%%Page: 33 34 +33 33 bop 164 315 a Fd("Protein)29 b(Sequence)g(Alignments:)58 +b(A)30 b(Strategy)f(for)h(the)f(Hier-)164 376 y(archical)g(analysis)164 +436 y(of)h(residue)f(conservation")164 496 y(Computer)g(Applications)g +(in)g(the)h(Biosciences,)164 556 y(9,)g(745-756.)949 +2607 y Fh(33)p eop +%%Trailer +end +userdict /end-hook known{end-hook}if +%%EOF diff --git a/sources/alscript/doc/alscript.tex b/sources/alscript/doc/alscript.tex new file mode 100644 index 0000000..9e699ab --- /dev/null +++ b/sources/alscript/doc/alscript.tex @@ -0,0 +1,1745 @@ +\documentstyle[12pt,times,html]{article} +\begin{document} +\parindent 0in +%\raggedright +\bibliographystyle{jmb} +\nocite{TitlesOn} +\begin{titlepage} +%\begin{singlespace} +\begin{center} +\begin{Huge} +\begin{bf} +\vskip 0.5in + +User Guide to + +ALSCRIPT - Sequence alignment to PostScript\\ + +Version 2.03\\ + +\end{bf} +\end{Huge} + +\vskip 0.5in + +\begin{large} +{\em Geoffrey J. Barton} +\vskip 0.5in + + +Laboratory of Molecular Biophysics\\ +University of Oxford\\ +Rex Richards Building\\ +South Parks Road\\ +Oxford OX1 3QU\\ +U.K.\\ + +\vskip 0.25in + +\end{large} +\vskip 0.25in +Tel: (44) 1865-275368\\ +Fax: (44) 1865-510454\\ +e-mail: gjb@bioch.ox.ac.uk + +\vskip 0.25in + +REFERENCE:\\ + Barton, G. J. (1993),\\ + ALSCRIPT a tool to format multiple sequence alignments\\ + Protein Engineering, Volume 6, No. 1, pp.37-40.\\ +\vskip 0.25in + +\end{center} +\end{titlepage} +\tableofcontents + +\section{Update History} +\begin{verbatim} + +VERSION 1.0 19 June 1992 +Version 1.1 26 June 1992 +Version 1.2 21 October 1992: Add multiple blocks per page option. +Version 1.3 15 November 1992: First Distribution. +Version 1.4 6 December 1992: Add Colour commands. +Version 1.4.1 1 February 1993: Small bug fixes - FULL RELEASE VERSION. +Version 1.4.2 15 February 1993: Make silent_mode toggle. +Version 1.4.3 1 March 1993: Fix bug in colour option. +Version 1.4.4 24 March 1993: Add mask features (should be version 1.5). +Version 1.4.5 25 May 1993: Include alsnum program in distribution. + 7 June 1993: Fix NO_NUMBERS bug in documentation + Change defaults for -q option to use MASK. + +Version 2.0 23 May 1995: Numerous changes and additions including the + option to colour backgrounds + differently, ommission of idents on + second and subsequent lines, helix, + strand and other special characters, + relative numbering, error checking of + ranges on input, bounding box, + screening, conservation colouring... +Version 2.03 5 June 1996: Small bug fixes - patches incorporated. + BACKGROUND_REGION and BOUNDING_BOX commands moved to + step 1 section. + +\end{verbatim} + +\section{Read This First - VERSION 2.0} + +This manual describes an interim release of ALSCRIPT that includes +many additional features over the previously distributed Version +1.4.5. I had hoped to make a lot more changes and improvements before +distributing the new version, however I have not had the time to do +this. I am distributing Version 2.0 since the new features have been +used in a number of published alignment figures. Please see +\hyperref{New Features in Version 2.0}{see the Section }{ }{new2} for details +of the new features. + +\section{Related Programs} + +The AMPS package (Barton, 1990). This performs multiple sequence +alignments and databank scanning. + +AMAS (Livingstone and Barton, 1993, CABIOS, 9, 745-756). Analysis of +Multiply Aligned Sequences. This package uses a sophisticated +set-based method to identify patterns of residue conservation in +multiple sequence alignments. + +All programs are available by anonymous ftp from geoff.biop.ox.ac.uk. +Please see the README file for details licencing and registration. +You can read manuals for the programs and some related papers at +http://geoff.biop.ox.ac.uk/. + +\section{Availability} + +ALSCRIPT is available free of charge for academic and non-commercial +purposes. Distribution is by anonymous ftp from geoff.biop.ox.ac.uk. +See the README file on the ftp server for details. You need to +register with G. J. Barton before downloading the software. + +\section{Installing ALSCRIPT} + +See the appropriate section for the computer type you are using: + +For PCs see \hyperref{386 MS-DOS installation}{see Section }{}{app4}. +For Unix see \hyperref{Unix installation}{see Section }{ }{app5}. +For VMS see \hyperref{VMS installation}{see Section }{ }{app6}. + +\section{Brief Description of ALSCRIPT} + +ALSCRIPT takes a multiple sequence alignment in AMPS (Barton \& +Sternberg, 1987, Barton, 1990) block-file format and a set of +formatting commands and produces a PostScript file that may be printed +on a PostScript laser printer, or viewed using a PostScript previewer +(e.g. Sun Microsystem's PageView program). CLUSTAL and GCG format +multiple alignment files may also be used (see below). ALSCRIPT is NOT a +multiple sequence alignment program, nor is it an alignment editor. + +Given a block-file and pointsize (character width/height), ALSCRIPT +calculates how many residues can be fitted across the page, and how +many sequences will fit down the page, it then prints the alignment at +the chosen pointsize on as many pages as are needed. Running ALSCRIPT with +a smaller or larger pointsize will automatically re-scale the alignment +to fit on fewer or more pages as appropriate. The actual page +dimensions may be re-set to any value, so if you have access to an A3 +PostScript printer, or typesetting machine, alignments can readily be +scaled to maximise the available space. + +Each output page has three basic regions. The left hand edge contains +identifier codes for each sequence. The main part of the page holds the +alignment, and the top part, the position numbers and tick marks. ALSCRIPT +commands make use of a character coordinate system for font changes, +and other formatting commands. Thus, any residue in the alignment may +be referred to by its sequence position number (x-axis) and sequence +number (y-axis), similarly, ranges of residue positions, or sequences +may also be defined in the character coordinate system. + +The basic ALSCRIPT commands allow the following functionality: + +Fonts: Any PostScript font at any size may be defined and used on +individual residues, regions or identifier codes. + +Boxing: Simple rectangular boxes may be drawn around any part of the +alignment. Particular residue types may be selected and automatically +"surrounded" by lines. For example, if the characters 'G' and 'P' are +selected, then lines will not be drawn between G and P characters, but +only where G and P border with other characters. + +Shading: Grey shading of any level from black to white may be applied +to any region of the alignment, either as a rectangular region, or as +residue specific shading. e.g. "shade all Cys residues between +positions 6 and 30" + +Text: Specific text strings may be added to the alignment at any +position and in any font or font size. + +Lines: Horizontal or vertical lines may be drawn to the left, right, top +or bottom of any residue position or group of positions. + +Colour: Characters or character backgrounds may be independently +coloured. + +The example block file "example1.blc" and command file "example1.als" +illustrate most of these commands in action. + +Although written with the aim of producing figures for journal +submission, ALSCRIPT may be used as a tool for interpreting multiple +sequence alignments. For example, the boxing, shading and font changing +facilities can be applied to highlight amino acids of a particular type +and thus draw attention to clusters of positive or negative charge, +hydrophobics, etc. + + +\section{New Features in Version 1.4.4} + +This version introduces the MASK family of commands which allows +boxing, shading etc to be applied according to the frequency of +occurence of the character types at each position in the alignment. +For example, it is possible to box positions where one character is +seen in more than N of the sequences. It is also possible to +box/shade etc the most frequently occurring character at each +position. Commands exist to select which characters will be used in +the calculation of frequencies and which will be excluded, thus boxing +can be based upon two or more character types at a position. MASK +commands also exist to show residues identical to one sequence in the +set. See the section on MASK below for details. + +NOTE: Although boxing according to the frequency of amino acids seen +at a position is a popular method of representation it is not usually +the most informative. An analysis that takes into account the +physico-chemical properties of the amino acids and also relates the +amino acid similarities to the overall similarity between the +sequences is more helpful in identifying functionally important +residues. The AMAS program (Livingstone and Barton, 1993) applies a +flexible hierarchical set-based approach to this problem. + +\section{New Features in Version 1.4.5 - Program alsnum} + +Version 1.4.5 includes the program "alsnum". This is a temporary solution to +the residue numbering problem. Ultimately, these functions will be included +as alscript commands. + +alsnum creates a set of TEXT commands that can be incorporated into an +alscript command file to place sequential numbers at any position in the +alignment. The numbers ignore gaps, so the numbering will correspond to +the specific sequence position rather than the alignment. + +To use the program: + +1. Decide where you want the numbers to be placed. For example, you might +want the numbers above the third sequence in the alignment. If so, make +an extra sequence space above the third sequence using the ADD\_SEQ\ command. + +2. Decide what is the number of the first residue of the sequence to be +numbered. This will not always be 1 since you may be aligning fragments +or domains. + +3. Decide the numbering interval (e.g. every 10th amino acid). + +4. Run the program. + +For example, if you want to add numbers according to sequence 37 of a +block file (junk.blc), calling the first residue of the sequence 12, +and with an interval of 5, and the numbers are to be placed at the +location of sequence 3 in the alignment. Type: + +alsnum 37 12 5 3 $<$ junk.blc $>$ junk.text + +5. Add the resulting TEXT commands from junk.text to your alscript +command file. + +\section{New Features in Version 2.0} +\label{new2} + +Error checking is now done on all ranges input. If you run ALSCRIPT +2.0 on a file that worked with ALSCRIPT 1.4.5, and it complains about +out of range numbers, then check your ranges carefully. If you think +you are right, then send me a minimal example of the problem and I +will investigate. Versions of ALSCRIPT before 2.0 would often work +happily with out of range numbers and produce perfectly OK output. + +The files {\bf ipns.als} and {\bf ipns.blc} show example command and +block file that use most of these new commands. See the {\em +examples} directory. + +\subsection{New Step 1 Commands} + +SCREENSIZE 120 + +Usually you should not need to change this value, it alters the +screening used by the printer. A value of 120 is used by default. On +most 300dpi black and white printers this gives much smoother greys +than the default used in earlier versions of ALSCRIPT. + +PIR\_SAVE\ filename + +Will cause the block file to be saved into the file ``filename'' in +PIR format. This can be useful for moving block file alignments to other +programs. + +MSF\_SAVE\ filename + +Will cause the block file to be saved into the file ``filename'' in +something that approximates GCG .msf format. {\em Warning! This has not +been fully tested.} + +NUMBER\_COLOUR\ 4 + +Sets the colour used for numbering at the top of the alignment (no +American spelling at the moment). In this example, colour number 4 has been +defined (See the DEFINE\_COLOUR command if you are not sure what this means). + +SINGLE\_PAGE + +If this is set, ALSCRIPT assumes everything will be plotted on one +page. At the moment, all this does is write the bounding box for the +figure, so encapsulating the PostScript. This {\em may} allow the +output of alscript to be imported into word processors etc, but +probably not all. + +ID\_ONLY\_ON\_FIRST\_LINE + +If this is present, then sequence identifiers will only be printed on +the first line of the alignment. Often this looks better for small +alignments than the default. + +BACKGROUND\_COLOUR\ 7 + +Sets the colour used for the background to the alignment. This can be +useful for preparing figures for projection. At the moment this only +works reliably when the SINGLE\_PAGE is also set. + + +BOUNDING\_BOX\ x\ y\ x1\ y1 + +Defines the bounding box for the figure. This is set in points (1/72 inch). +NOTE: In version 2.0 this was a STEP 2 Command. + +BACKGROUND\_REGION\ x\ y\ x1\ y1 + +Defines the region to colour as background - the default is set up for A4 paper +so US users may have to fiddle with this. Values are points (1/72 inch). +NOTE: In version 2.0 this was a STEP 2 Command. + +\subsection{New Step 2 Commands} + + +COLOUR\_TEXT\_REGION\ x\ y\ x1\ y1 colour + +Sets the colour for TEXT command output. Similar syntax to COLOUR\_REGION, FONT\_REGION etc. + +COLOUR\_LINE\_REGION\ x\ y\ x1\ y1 colour + +Set the colour for LINEs in a region. + +CALCONS\ x\ y\ x1\ y1 + +Calculate conservation values according to Zvelebil {\em et al.} for the designated +region. (See Livingstone \& Barton 1993 for details and further refs) + +MASK\ CONSERVATION\ cutoff + +If CONSCAL has been used, then mask residues according to the conservation cutoff. + +e.g. MASK CONSERVATION 10 would mask all identities, MASK CONSERVATION 6 would +mask reasonably conserved positions. See examples for more on this command. + +HELIX\ x1\ y\ x2 + +Draw a helix from x1 to x2 of sequence y. + +STRAND\ x1\ y\ x2 + +Draw a strand from x1 to x2 of sequence y. + +COIL\ x1\ y\ x2 + +Draw a coil (horizontal line) from x1 to x2 of sequence y. + +RELATIVE\_TO + +Set reference numbers to work relative to sequence number . This +means that in all subsequent commands, ALSCRIPT will translate your x +values into absolute position values in the alignment. This is {\em +extremely} useful since you can annotate your alignment using your +favourite sequences as a reference point. You no longer have to +translate every x position into the alignment position. + + is optional. If present, it specifies what the first +residue in the displayed sequence is. For example, you may be showing +residues 200-500 of a sequence, so would be 200 rather than +the default of 1. {\em Warning - this is a very new feature and +bounds checking is not fully enabled for it}. + +You can use RELATIVE\_TO several times in the command file to annotate +different sequences. RELATIVE\_TO 0 resets to the ``normal'' +alignment numbering. + +\subsection{New special TEXT commands} + +Some special TEXT commands have been added to allow drawing of alternative +shapes etc. In fact this is how the HELIX, STRAND and COIL commands are implemented. The text commands are all prefixed by an @ symbol. + +e.g. TEXT 3 6 ``@fuparrow'' + +will draw a filled up arrow at position 3,6. + +The alternative text commands are: + +@leftarrow - an open left pointing arrow. + +@fleftarrow - a filled left pointing arrow. + +@uparrow - an open up pointing arrow. + +@fuparrow - a filled up pointing arrow. + +@downarrow - an open down pointing arrow. + +@fdownarrow - a filled down pointing arrow. + +@circle - an open circle. + +@fcircle - a filled circle. + +I plan to make this option more flexible in the near future. + +\section{Running ALSCRIPT} +\subsection{Basic Use} + +I recommend you read through this section, then scan the commands in +\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1} to get a feel for what ALSCRIPT can do. + +See \hyperref{Alternative ways of invoking ALSCRIPT}{Section }{for +alternative methods of invoking ALSCRIPT}{app7}. In this section, the +interactive method is described. The QUICK START method shown in +\hyperref{alternative ways of invoking ALSCRIPT}{Section }{ }{app7} +is useful to format a sequence alignment quickly +using standard pointsize and shading. + +ALSCRIPT is designed to work with AMPS block file format multiple +alignments. If you have a multiple alignment generated by CLUSTAL V +or the GCG package, then it must be translated to AMPS block file +format. + +To translate a GCG .MSF file: Type: msf2blc. +To translate a CLUSTAL PIR format file, or any PIR format file: clus2blc. + +Both programs prompt for the name of an input file, and an output +block file name. A good convention to follow is to name all blockfiles +with the extension ".blc". + +To run ALSCRIPT simply type: + +alscript + +you will then be prompted for the name of the ALSCRIPT command file. +Having typed the filename, the commands will be executed as you have +specified. + +A Simple Command File (example.als) + +The file example.blc contains a small multiple sequence alignment. +The following ALSCRIPT command file will convert this into a +PostScript alignment file in 12 point Helvetica. + +\begin{verbatim} +#Comments in ALSCRIPT command files start with a # +# +#Commands are free format - separated by blank, tab or comma characters +# +BLOCK_FILE example.blc #define the block file to format +OUTPUT_FILE example.ps #where to put the result +LANDSCAPE #landscape paper orientation +POINTSIZE 12 #12 point default pointsize +DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica +SETUP #Tell the program to get on with it. +\end{verbatim} + +Now try changing the POINTSIZE value to 5 ALSCRIPT will re-format the +alignment to make best use of the available paper. + +These are all STEP 1 commands - they refer to overall layout, and system +settings - for example, the paper size or maximum sequence length. +Other commonly used STEP 1 commands are IDENT\_WIDTH\ which reserves more +or less width for the sequence identifier codes, NUMBER\_SEQS\ which adds +a number to each sequence and LINE\_WIDTH\_FACTOR\ which allows the +thickness of all boxing lines to be adjusted. See +\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1} +for details of these and all other STEP 1 commands. + +The simple example outlined above can be modified with a variety of +STEP 2 commands. + +for example file example2.als: + +\begin{verbatim} +# FILE example2.als +# +#Commands are free format - separated by blank, tab or comma characters +# +BLOCK_FILE example.blc #define the block file to format +OUTPUT_FILE example2.ps #where to put the result +LANDSCAPE #landscape paper orientation +POINTSIZE 12 #12 point default pointsize +DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica +DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica +DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic +NUMBER_SEQS #Number the sequences at left hand side +SETUP #Tell the program to get on with it. +# +#step 2 commands come after the SETUP command +# +#Here are some examples... +# +SURROUND_CHARS GP ALL #draw lines around all G and P +SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6 +BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20 +FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters +ID_FONT ALL 1 #set identifiers in font 1 + +\end{verbatim} + +There are many possible ways of combining these commands and the +others shown in +\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1}. +In general, if you apply multiple +commands to the same residue, the effect of the last applied command +persists where there would otherwise be a conflict. Thus the +intersection of two overlapping SHADed regions would be shaded +according to the second SHADE command, not some mixture of the two. +Similarly for FONT commands. BOX and SURROUND commands behave in the +opposite sense, all BOXing and SURROUNDing persists regardless of how +many commands you issue. This makes it possible for example, to +SURROUND two different sets of residues as follows: + +\begin{verbatim} + +SURROUND_CHARS DE ALL +SURROUND_CHARS DEHKR ALL + +\end{verbatim} + +This would result in D and E characters being partitioned from the rest as well +as D E H K R characters (see Example output). + +\subsection{More complex effects - Text Lines, and Masks} + +Text, lines and masking are meant to be used to annotate the multiple +alignment. The TEXT command allows any piece of text to be located +anywhere on the alignment. Clearly, however it makes little sense to +superimpose the text over the alignment though this can be done! Accordingly, +you must first make a space to put the text in. Usually, this will be a +few lines below the multiple sequence alignment, but you may want to +add text at the top, or somewhere in between two sequences. You can make +space in two ways. Either by editing the block-file to introduce "dummy" +sequences at the locations you want, or by making use of the ADD\_SEQ\ +command. + +The ADD\_SEQ\ command has two arguments, the sequence after which you want +further sequences to be added, and how many blank sequences you need. +Thus, we can reserve space for 5 lines of text underneath a 10 sequence +multiple alignment with the following command. + +ADD\_SEQ\ 10 5 + +we can then put text below the alignment at the 20th residue. + +TEXT 20 13 "Active Site His" + +or any other position. + +Similarly, we could draw a vertical line to point out which residue we mean + +LINE LEFT 20 13 11 + +And change the font of the text to number 7 (whatever that has been set to): + +FONT\_RESIDUE\ 20 13 7 + +You can have multiple ADD\_SEQ\ commands, but they must occur in sequence order. +Thus: + +\begin{verbatim} +ADD_SEQ 0 5 +ADD_SEQ 5 12 +\end{verbatim} +is legal. +But +\begin{verbatim} +ADD_SEQ 5 12 +ADD_SEQ 0 5 +\end{verbatim} + +Is NOT!! NO CHECKING IS performed by the program for this error - so beware! + +Note that add\_seq\ commands refer to the actual sequence number as +implied by the block file, not the number after applying the add\_seq\ +command. Thus, for a four sequence block file, if you want to add +space for three sequences before sequence 1 and two sequences after +sequence 3, the commands would be: + +\begin{verbatim} +ADD_SEQ 0 3 +ADD_SEQ 3 2 +\end{verbatim} + +Text added with the TEXT command will not be split across page breaks, +so you may in some circumstances need to fiddle a little with the +location/pointsize for the text to get the desired result. + +Masking is a technique for drawing irregular shaped outlines, or +shaded regions - this should not be confused with the MASK family of commands +described below. For example a histogram can be added to the bottom of +an alignment by first defining some dummy sequences in the block-file +that have letters building up the shape of the histogram, then using +the SURROUND\_CHARS\ or SHADE\_CHARS\ commands together with the SUB\_CHARS\ +command to produce the desired effect. An example of this operation +being used to show frequencies of secondary structure predictions is +shown in example1.als and in the Protein Engineering paper. + +\section{Using Colour} + +Version 1.4 includes commands to allow the independent colouring of +characters, or their backgrounds. Colours are defined in a similar +manner to fonts using the DEFINE\_COLOUR\ command (American spelling also +allowed). For example: + +DEFINE\_COLOUR\ 7 1 0 0 + +defines colour number 7 to be red - see +\hyperref{ALSCRIPT Command Summary}{Section }{ }{app1} +for full details of +this command. Colours 99 and 100 are pre-defined to white and black. +ALSCRIPT assumes the paper colour is white. + +The command to colour the text of a character or text string is: + +CCOL\_CHARS\ + +the command to colour the background of a character is + +SCOL\_CHARS\ + +both have similar syntax to the FONT\_CHARS command. + +COLOUR\_REGION\ and COLOUR\_RES\ have similar syntax to SHADE\_REGION\ and +SHADE\_RES. + +An example command file that uses colour is shown in example3.als. + +\section{The MASK command family} + +The idea behind the MASK command is to build up a set of character +positions that will subsequently be boxed, shaded, set in a particular +font, etc. For example, lets say we want to box the most frequently +occuring character at each position in an alignment. + +The command + +mask SETUP + +tells ALSCRIPT to prepare a mask. + +mask FRE ALL + +specifies that the most frequently occuring character at each position +in the alignment will be masked. This command can be restricted to a +region of the alignment using: mask FRE sx sy ex ey, where sx etc +define the region in the same way as for font\_region\ and other commands. + +mask BOX ALL + +Tells ALSCRIPT to create the boxing lines that will separate the masked +characters from non-masked characters - this command may also be +restricted to a region of the alignment. + +The mask can be reset for re-use using the command: + +mask RESET + +Two further commands define which characters can be used when +calculating the mask. This allows gap-characters, or other amino acids +to be excluded from the calculation to avoid unwanted boxing. + +mask LEGAL "AVLI" + +defines the AVL and I as the only characters that will be used when +calculating the mask. + +mask ILLEGAL ".-\_" + +defines .- and \_\ as characters that will not be used when calculating +the mask. NOTE: the blank character " " cannot be defined in this way. +To avoid boxing " " characters substitute blanks for something else +(using SUB\_CHARS),\ calculate the mask, then substitute back. + +\subsection{Summary of mask commands} + +mask SETUP +\# Prepares for masking + +mask LEGAL $<$qstring$>$ +\# defines characters to include in ID or FRE calcs - optional. + +mask ILLEGAL $<$qstring$>$ +\# defines characters to exclude in ID or FRE calcs - optional. + +mask ID ALL N +\# Calculates a mask that flags the character that occurs at least N +times at a position. The word ALL can be substituted by four numbers +that define a region of the alignment. + +mask FRE ALL +\# calculates a mask that flags the most frequently occuring amino acid +at each position. ALL may be replaced by four numbers defining a region +of the alignment. + +Multiple mask FRE or mask ID commands may be applied, using different +LEGAL and ILLEGAL character definitions. In this way more complex +effects can be built up. + +The mask command also allows characters that are identical to one +sequence to be masked. + +mask AGREE ALL N + +will mask all positions that are identical to the Nth sequence. Thus, +for sequences that are very similar to a newly sequenced sequence, all +characters identical to the new sequence can be boxed or shaded, or set +in a different font or colour etc... + +mask NOT ALL + +allows the mask to be inverted. Thus, all positions that are NOT in the +mask now form the mask. So, having done a mask AGREE, a mask NOT will +allow the positions that are not identical to the selected sequence to +be highlighted or substituted. + +mask SUB ALL $<$char$>$ + +substitutes all characters in the mask with the character $<$char$>$. + +mask REGION ALL +applies a mask to all residues in the defined region. + +The following effects can now be applied to the masked characters: + +mask BOX ALL +\# boxes the masked residues - ie surrounds them by lines. + +mask SHADE ALL $<$grey$>$ +\# shades the masked residues by grey value. + +mask FONT ALL $<$fontnum$>$ +\# Uses font fontnum to output the masked residues. + +mask INVERSE ALL +\# Inverts the masked characters - ie outputs them in white. + +mask CCOL ALL $<$colnum$>$ +\# outputs the masked characters in the defined colour. + +mask SCOL ALL $<$colnum$>$ +\# outputs the backgrounds of the masked characters in the defined +colour. + +In all commands, the word ALL can be replaced by four numbers defining +the region to which the command is applied. + +mask RESET +\# resets the entire mask for re-use + + +\section{Printing ALSCRIPT Files} + +ALSCRIPT produces files in PostScript which may be printed on a PostScript +printer (e.g. an Apple LaserWriter). If you don't have a PostScript printer, +then you may still be able to use ALSCRIPT if you get hold of the +GhostScript software. This is a free package that interprets PostScript +commands and can produce output on a large number of different types of +printer. GhostScript runs on most hardware types (including PCs) and can also +display output to the screen. The package can be obtained from many different +sites on the Internet (In the UK try src.doc.ic.ac.uk). + +The actual command you need to type to send a PostScript file to the printer +will depend on your system. Consult your system manager for help. + +Be warned, ALSCRIPT can create extremely large PostScript files if +lots of boxing and shading is done on big alignments. On older +printers such output may take a long time to process. + +\section{Conclusion} + +ALSCRIPT provides a powerful set of formatting and editing commands +specifically tailored for multiple sequence alignments. It is best +used in conjunction with a PostScript previewer such as Sun's +PageView or GhostView since this allows the effect of changing a command to be +seen quickly. In the absence of such a tool, simpler effects can be +tested out without destroying too many trees in the Laser Printer! + +Like most programs, ALSCRIPT is evolving as I find new problems to display, so +if you have any suggestions - I shall endeavour to include them in a later +version. + +\section{Appendices} + +\subsection{ALSCRIPT Command Summary} +\label{app1} + +WARNING: Very little error checking is performed on command input. If +you give the wrong number of arguments to a command, then unexpected +things may happen, or the program will crash very inelegantly. I hope +to fix this in the next version of the program, in the meantime, make sure +you give the correct number of arguments to each command. + +All commands up to the first space character may be entered in UPPER +or lower case or MiXEd case. Qualifiers for commands (e.g. REL) must +be written in UPPER case. + +Command Reference: + +\begin{verbatim} + = enter an integer (e.g. 240) + = enter a floating point number (e.g. 0.45) + = enter a string (e.g. ARNDql) + = enter a quoted string (e.g. "Active Site") + = enter a single character. +\end{verbatim} + +\subsubsection{STEP 1 COMMANDS} + +These all refer to either system settings - e.g. the maximum allowed +sequence length, or to general page layout features. e.g. the longest +and shortest side of the paper on which you are plotting. + +\subsubsection{REQUIRED STEP 1 COMMANDS} + +BLOCK\_FILE\ $<$string$>$ + +Gives the name of the file that contains the multiple sequence alignment +to be formatted. File names should be fully qualified i.e. not +relative to the current directory. If no block file command is given, +ALSCRIPT will expect to read the block file from standard input. + +OUTPUT\_FILE\ $<$string$>$ + +Defines the output file name. This command should be near the beginning of +the command list. +e.g. OUTPUT\_FILE\ Figure1.ps + +You MUST define an output file unless the -p option +(See \hyperref{Alternative ways of invoking ALSCRIPT}{Section }{ }{app7}) +is used. + +DEFINE\_FONT\ $<$int$>$ $<$string$>$ ($<$int$>$/DEFAULT)/(REL $<$float$>$) + +Defines a font to use later: +e.g. +\begin{verbatim} +DEFINE_FONT 0 Helvetica 10 +DEFINE_FONT 2 Times-Roman 2 +\end{verbatim} + +defines font number 0 to be 10 point Helvetica, and font number 2 to be +2 point Times-Roman. Font 0 is always used as the default font. You MUST +define at least the font 0 font. + +DEFINE\_FONT\ 3 Times-Roman DEFAULT + +sets font 3 to be Times-Roman at whatever the default pointsize +is as set by the POINTSIZE command. + +DEFINE\_FONT\ 4 Helvetica REL 0.5 + +sets font 4 to be helvetica at half the default pointsize. + +NOTE: Font names must be written exactly as shown in +\hyperref{PostScript Fonts}{Section }{ }{app3}. + + +SETUP + +Signals the end of the STEP 1 commands. + +\subsubsection{OPTIONAL STEP 1 COMMANDS} + + +ADD\_SEQ\ $<$int$>$ $<$int$>$ + +Allows extra sequence positions to be created in an existing alignment. This +permits additional annotations to be interspaced either above, below, or +anywhere in the middle of an alignment. For example: + +ADD\_SEQ\ 0 10 + +would create an additional 10 sequences - all set to the blank character +before the first sequence in the block file that has been read in. + +ADD\_SEQ\ 3 1 + +would add an extra sequence after sequence 3. + +IMPORTANT: If you use the ADD\_SEQ\ facility to add sequences anywhere except +after the last sequence, then remember that the sequence +numbers will alter. All formatting commands that follow this command +must use the new sequence numbering. Thus in the first example: + +ADD\_SEQ\ 0 10 + +what was sequence number 1 becomes sequence 11. Sequences 1-10 are +the new blank sequences to be used for annotation. Note that the +sequence numbers only change for commands AFTER the SETUP command, +thus, multiple add\_seq\ commands refer to the sequence number as +implied by the block file. + +POINTSIZE $<$int$>$ + +Defines the pointsize to be used to scale the plot and space the characters. +Default is 10 point. + +NUMBER\_SEQS + +If present, then the sequence number is output with the identifier +code. This is useful for finding the coordinates of residues to box +or otherwise highlight. + +LANDSCAPE + +Specifies that alignments will be plotted with the longest paper axis +horizontal. (Can get longer alignments on a page this way). + +PORTRAIT + +Specifies that alignments will be plotted with the longest paper axis vertical +(can get more sequences on a page this way). + +IDENT\_WIDTH\ $<$int$>$ + +Units are characters. + +Reserves $<$int$>$ characters at left of every page for plotting +identifiers. Note that not all this space need be used, if a smaller +pointsize is used to plot out the identifier codes, than is used for +the main alignment. + +LINE\_WIDTH\_FACTOR\ $<$float$>$ + +Value greater than 0 that scales the default line width. The linewidth +is obtained by multiplying the pointsize by this factor. + + +X\_SPACE\_FACTOR\ $<$float$>$ + +Y\_SPACE\_FACTOR\ $<$float$>$ + +This determines the spacing between adjacent residues in the X and Y +directions. The spacing is calculated as: POINTSIZE + POINTSIZE * +X\_SPACE\_FACTOR or POINTSIZE + POINTSIZE * Y\_SPACE\_FACTOR\ as +appropriate. Defaults are 0.2 and 0.0 respectively. + +X\_SHIFT\_FACTOR\ $<$float$>$ + +Y\_SHIFT\_FACTOR\ $<$float$>$ + +These determine the shift relative to the residue drawing position +that is given to the boxing lines. The shift is calculated as +follows + +(POINTSIZE + POINTSIZE * X\_SPACE\_FACTOR)\ * X\_SHIFT\_FACTOR\ +similarly for Y\_SHIFT\_FACTOR. + +The defaults are 0.3 and 0.0 respectively. + +Fiddling with the X\_SPACE/SHIFT\ values is useful to fine tune the +appearance of the alignment. + + +MAX\_INPUT\_LEN\ $<$int$>$ + +Units are characters. +Defines the maximum number of characters possible in the input line +length. This must be greater than the maximum number of sequences +(MAX\_NSEQ). + +e.g. MAX\_INPUT\_LEN\ 600 + +Increases the default value of 500 characters to 600 characters. + +MAX\_NSEQ\ $<$int$>$ + +Units are characters. Defines the maximum number of sequences that +may be read by the program. This parameter has a large default (500). +You may need to reduce it on computers with small memories. + +MAX\_ILEN\ $<$int$>$ + +Units are characters. +The maximum length allowed for a sequence identifier code. + +MAX\_SEQ\_LEN\ $<$int$>$ + +Defines the maximum length allowed for a sequence alignment - this may +need to be reduced from the 8000 default value on smaller computers. + +X\_OFFSET\ $<$int$>$ +Units of points (1/72 inch). + +Defines the offset along the X-axis that the alignments will be shifted prior +to printing. Fiddle with this value to get a nice offset from the bottom left +hand corner of the page if your page size is not A4. + +Y\_OFFSET\ $<$int$>$ +Units of points (1/72 inch). + +As for X\_OFFSET,\ only Y axis. + +MAX\_SIDE\ $<$int$>$ +Units of inches. + +Defines the length of the longest side of the printer page. + +MIN\_SIDE\ $<$int$>$ +Units of inches. + +Defines the length of the shortest side of the printer page. + +VERTICAL\_SPACING\ $<$int$>$ + +Defines the vertical spacing in character units between blocks of sequences +when more than one block will fit on a page - default is 0. + + +DEFINE\_COLOUR\ $<$int$>$ $<$float$>$ $<$float$>$ $<$float$>$ + +DEFINE\_COLOR + +Defines a colour - the first number is a number by which the colour will +be referred. The following three numbers are the intensities of red, +green and blue respectively. Thus: + +DEFINE\_COLOUR\ 1 0 0.2 0.8 + +sets colour number 1 to be a colour with no red, 0.2 green and 0.8 blue. +The exact appearance of this colour will depend on the output device. +If you find suitable combinations of colours for your printer, then +please let me know and I shall distribute your suggestions with the +program. + +DO\_TICKS + +If present, then tick marks are drawn below the numbers at the top of the page. +Otherwise no ticks are shown. + +NUMBER\_INT\ $<$int$>$ + +Specifies the interval for writing residue position numbers. Default is 10 + +NO\_NUMBERS + +Switches all residue numbering off. + + +\subsubsection{STEP 2 COMMANDS} + + +All these are optional formatting commands. + +IMPORTANT PLEASE READ THIS NOTE: + +For those commands that accept region definitions (e.g. SURROUND\_CHARS) it +is easiest to think of the region being defined in terms of X and Y +coordinates, where X is the sequence residue coordinate and Y is the +sequence number coordinate. Thus 3 7 means the 3rd residue in sequence 7. +3 7 12 42 means the rectangular box bounded by residue 3 of sequence 7 and +residue 12 of sequence 42. + +SURROUND\_CHARS\ $<$string$>$ ALL + +Draw lines round, but not between the characters that are in the string. +e.g. + +SURROUND\_CHARS\ GP ALL + +will draw lines round all G and P characters in the alignment, but not +between adjacent G and P characters. + +SURROUND\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +Similar command, but the surrounding is restricted to the region defined by +the four integers. + +e.g. + +SURROUND\_CHARS\ ILVW 3 12 7 32 + +would surround ILVW characters that occur in the region defined +from residue positions 3-7 of sequences 12 to 32. + +SHADE\_CHARS\ $<$string$>$ ALL $<$float$>$ + +Shade all characters in the $<$string$>$ by the grey value given by $<$float$>$. +e.g. + +SHADE\_CHARS\ GP ALL 0.5 + +would shade all G and P characters in the alignment by the grey value 0.5. + +SHADE\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$float$>$ + +restricts the shading to the region defined by the four integers. Thus + +SHADE\_CHARS\ ILVW 3 12 7 32 0.7 + +would shade I L V and W characters from residues 3-7 of sequences 12-32 +inclusive with a grey value of 0.7. + +FONT\_CHARS\ $<$string$>$ ALL $<$int$>$ + +e.g. + +FONT\_CHARS\ GP ALL 7 + +would use font 7 to write out all G and P characters. Font 7 MUST have been +defined using the DEFINE\_FONT commands above. + +FONT\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +Similar to previous command, but restricts the effect to the region defined +by the first four integers. The font must have been defined by the +DEFINE\_FONT\ command. + +e.g. + +FONT\_CHARS\ ILVW 3 45 9 70 7 + +Would set the font to 7 for I L V and W characters for residues 3-9 of +sequences 45-70 inclusive. The font must have been defined by the +DEFINE\_FONT\ command. + +FONT\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +Define the font to use throughout the region specified by the first four +integers. + +e.g. + +FONT\_REGION\ 3 12 20 40 10 + +Use font 10 for residues from residues 3-20 of sequences 12-40. The font +must have been defined using the DEFINE\_FONT command. + +FONT\_RESIDUE\ $<$int$>$ $<$int$>$ $<$int$>$ + +Set the font for use with a single residue position - most useful when used +with the TEXT command. + +e.g. + +FONT\_RESIDUE\ 3 7 2 + +Use font 2 for residue 3 of sequence 7. Font 2 must have been defined using +the DEFINE\_FONT\ command. + +LINE $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +There are four commands of this type for drawing horizontal or vertical lines +on the alignment. + +LINE LEFT $<$int$>$ $<$int$>$ $<$int$>$ + +Draw a line to the left of the character positions indicated. + +e.g. + +LINE LEFT 3 12 24 + +Draw a vertical line starting at residue 3 of sequence 12 and ending at +residue 3 of sequence 24. + +LINE TOP 3 12 24 + +Draw a horizontal line above the character positions from residue 3 of +sequence 12 to residue 24 of sequence 12. + +Similar commands are: + +LINE BOTTOM $<$int$>$ $<$int$>$ $<$int$>$ Draw a line at bottom of character position. + +LINE RIGHT $<$int$>$ $<$int$>$ $<$int$>$ Draw a line at right of character position. + +BOX\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +Draw a box around the region indicated by the four integers. + +e.g. + +BOX\_REGION\ 2 5 30 7 + +Would box from residue 2 of sequence 5 to residue 30 of sequence 7. + +SHADE\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$float$>$ + +Shade the region indicated by the integers with the grey value shown by the +float. +e.g. + +SHADE\_REGION\ 30 40 35 46 0.2 + +Would shade from residue 30-35 of sequences 40-46 with a grey value of 0.2. + +SHADE\_RES\ $<$int$>$ $<$int$>$ $<$float$>$ + +Shade just one amino acid with the grey value. + +e.g. + +SHADE\_RES\ 3 4 0.7 + +Shades residue 3 of sequence 7. (Note: this can also be achieved with the + +SHADE\_REGION\ command, but requires 2 extra numbers) + +TEXT $<$int$>$ $<$int$>$ $<$qstring$>$ + +Place the text string at the location indicated. + +e.g. + +TEXT 30 70 "Active Site His" + +would put the text Active Site His starting at position 30 of sequence +70. (Use FONT\_RESIDUE\ or FONT\_REGION\ commands to set the font of the +text). Text added with the TEXT command will not be split across page +breaks, so you may in some circumstances need to fiddle a little with +the location/pointsize for the text to get the desired result. + +ID\_FONT\ ALL $<$int$>$ + +Set the font for all identifier codes to the font number shown by $<$int$>$. +e.g. + +ID\_FONT\ ALL 3 + +Would set all the identifier codes to font 3. + +ID\_FONT\ $<$int$>$ $<$int$>$ + +Set the font for a specific identifier to font number. +e.g. + +ID\_FONT\ 12 4 + +Use font 4 for the identifer of sequence 12, default font for all other +identifiers. + +SUB\_CHARS\ ALL $<$char$>$ $<$char$>$ + +Substitute the characters indicated. + +e.g. + +SUB\_CHARS\ ALL + * + +would change all occurences of + to * in the alignment. + +SUB\_CHARS\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$char$>$ $<$char$>$ + +restrict the substitution to the region shown. + +e.g. + +SUB\_CHARS\ 1 1 7 8 \% * + +would substitute * for \% from residue 1-7 of sequences 1-8. +NOTE: To substitute for or with the space character use the word SPACE. +e.g. to change all space characters to -. + +SUB\_CHARS\ ALL SPACE - + +SUB\_ID\ $<$int$>$ $<$qstring$>$ + +Replace the numbered identifier by the string. +e.g. + +SUB\_ID\ 34 "Predicted Secondary Structure" + +would replace whatever the identifier of sequence 34 was, by the text shown. +This is useful when used in conjunction with the ADD\_SEQ\ command shown under +the STEP 1 commands. + +INVERSE\_CHARS\ $<$string$>$ ALL/Range (similar syntax to FONT\_CHARS\ but no +font number) + +Print the selected characters in white. This clearly will only work +if you first use the SHADE\_CHARS command to shade the characters with +something other than white. + +CCOL\_CHARS\ $<$string$>$ ALL $<$int$>$ + +Colour all characters in the $<$string$>$ by the colour defined by $<$int$>$. + +e.g. + +CCOL\_CHARS\ GP ALL 12 + +would colour all G and P characters in the alignment by the colour 12. +This colour MUST have been defined by the DEFINE\_COLOUR\ command. + +CCOL\_CHARS\ $<$string$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +restricts the colouring to the region defined by the four integers. Thus + +CCOL\_CHARS\ ILVW 3 12 7 32 7 + +would colour I L V and W characters from residues 3-7 of sequences 12-32 +inclusive with the colour 7. + +SCOL\_CHARS:\ This has identical syntax to SCOL\_CHARS,\ but colours the +background of the character, rather than the letter itself. + +COLOUR\_REGION\ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ $<$int$>$ + +COLOR\_REGION + +Colour the region indicated by the integers with the colour number given +as the last number. + +e.g. + +COLOUR\_REGION\ 30 40 35 46 2 + +Would colour from residue 30-35 of sequences 40-46 with the colour 2. + +COLOUR\_RES\ $<$int$>$ $<$int$>$ $<$int$>$ + +Colour just one amino acid with the defined colour. + +e.g. + +COLOUR\_RES\ 3 4 7 + +Colours residue 3 of sequence 7. (Note: this can also be achieved with the +COLOUR\_REGION\ command, but requires 2 extra numbers) + + +\subsection{AMPS Block file format} +\label{app2} + +The first part of a block-file contains the identifier codes of the +sequences that are to follow. Each code is prefixed by the $>$ symbol, codes +must not contain spaces. + +e.g. +\begin{verbatim} +>HAHU +>Trypsin +>A0046 +>Seq1 + +\end{verbatim} + +etc. + +ALSCRIPT counts the number of $>$ symbols in the beginning of the file +until a * symbol is found. The * signals the beginning of the +multiple alignment which is stored VERTICALLY, thus columns are +individual sequences, whilst rows are aligned positions. The * symbol +must lie over the first sequence. A further star in the same column +signals the end of the alignment. ALSCRIPT uses the number of $>$ +symbols at the beginning of the file to work out how many columns to +read from the * position. It is therefore important that the only $>$ +symbols in the file are those that define the identifiers, and the +only * symbols are those defining the start and end of the multiple +alignment. The block file can contain additional text, providing that +there are no more $>$ or * symbols in the file than those used to define +the identifiers or alignment start and end. + +A simple, small block-file is shown here. + +\begin{verbatim} +>Seq_1 +>A0231 +>HAHU +>Four_Alpha +>Globin +>GLobin_C +* +ARNDLQ +AAAAAA +PPPPPP +PP PPP +WW WWW +LLLLLL +IIVVLL +* +\end{verbatim} + + +\subsection{PostScript Fonts} +\label{app3} +\begin{verbatim} +Times-Roman, +Times-Italic, +Times-Bold, +Times-BoldItalic, +Helvetica, +Helvetica-Oblique, +Helvetica-Bold, +Helvetica-BoldOblique, +Courier, +Courier-Oblique, +Courier-Bold +Courier-BoldOblique, +AvantGarde-Book, +AvantGarde-BookOblique, +AvantGarde-Demi, +AvantGarde-DemiOblique, +Bookman-Demi, +Bookman-DemiItalic, +Bookman-Light, +Bookman-LightItalic, +Helvetica-Narrow, +Helvetica-Narrow-Bold, +Helvetica-Narrow-BoldOblique, +Helvetica-Narrow-Oblique, +NewCenturySchblk-Roman, +NewCenturySchlbk-Bold, +NewCenturySchblk-Italic, +NewCenturySchblk-BoldItalic, +Palatino-Roman, +Palatino-Bold, +Palatino-Italic, +Palatino-BoldItalic +ZapfChancery-MediumItalic. +Symbol +\end{verbatim} + +\subsection{386 DOS installation} +\label{app4} + + +IMPORTANT - The programs on this disk will ONLY WORK on a PC with a 386 +or better processor. See the Technical Notes section for details of why. + +Directions: + +\begin{enumerate} + +\item + Create a directory on your hard disk. + e.g. mkdir ALSCRIPT. + +\item + Copy the Contents of the floppy disk into this directory. +\begin{verbatim} + e.g. copy a:*.* c:\alscript. +\end{verbatim} + +\item + Edit your AUTOEXEC.BAT file and add +\begin{verbatim} + C:\ALSCRIPT to your path. +\end{verbatim} + +\item + Edit your AUTOEXEC.BAT file and add the following two lines. + set DOS4GVM=@ALSCRIPT.VMC + set DOS4G=quiet +\end{enumerate} + +The first line is an instruction to read instructions from the file +ALSCRIPT.VMC. This sets up a permanent swap file on your hard disk. +By default, the swap file is about 12MBytes in size. If you do not have +this much free space on your disk, then edit the ALSCRIPT.VMC file +to reduce the swap file size, or alternatively, do not put this line +in your autoexec.bat. + +The programs will run without this swap file, but you will be limited in + the size of alignment you can process by the amount of RAM you have +installed. I have only tested this program on a 486/33 with 8MBytes RAM +and a 386/33 with 4MBytes so I do not know the practical limitations of +machines with smaller memories. Any feedback would be appreciated. + +5. Type AUTOEXEC.BAT to initialise the changes, or better still, reset +the computer. + +6. You should now be able to run all three programs in the package from +anywhere on your disk. msf2blc, clus2blc and alscript. If you get +memory allocation errors when you try to run alscript, then use the +MAX\_NSEQ\ and MAX\_SEQ\_LEN\ commands to reduce the default limits. If the +program still won't run, then think about buying some more memory!! + +The programs msf2blc and clus2blc should run OK, but if you try to +process alignments that are too large for your computer, you may get a +"malloc error" which will stop the program. If this happens and you are +not using the virtual memory option discussed above, then try adding the +line set DOS4GVM=@filename to your autoexec.bat file. If you +don't have enough disk space to do this, then buy a bigger disk, or more +memory. + +\subsection{TECHNICAL NOTES} + +The executables included in this package were compiled with the WATCOM C +compiler. This is a full 32 bit compiler that makes good use of the 386 +processor and does not work on the 16 bit 286. It also has the +advantage of allowing the flat memory model to be used. In practice +this means that porting programs like alscript from Unix computers like +the Sun, is straightforward. In order to access the memory of the +computer in this way, an extra program called a dos extender is required +- this is called DOS4GW.EXE. DOS4GW is automatically invoked every time +you run one of the programs and is responsible for managing the memory and +creating the swap file discussed above. + +\subsection{Unix Installation} +\label{app5} + +ALSCRIPT is distributed with executables for Sun (SunOS 4.1.3), Silicon +Graphics (IRIX 5.3), DEC ALPHA OSF/1 and Sun Solaris (2.4). The executables +are stored in the subdirectories bin/sun, bin/sgi, bin/osf and bin/sol. If +these are OK for your system, then just add the apporpriate directory to your +path, or put links to /usr/local/bin or somewhere that is on all users paths. + +The source code for ALSCRIPT is contained in a directory hierarchy. +The top directory contains a README file and the BUILD script. +Subdirectories are: {\bf examples} which contains example command and +alignment files, {\bf doc} which contains \LaTeX and PostScript copies +of the manual - a subdirectory of this contains an HTML version of the +manual, and {\bf src} which contains the source code and Makefiles for +the package. There may also be a directory called {\bf bin}. If +present this will contain subdirectories with executables for the +programs in the package. Makefiles to build alscript, msf2blc, +clus2blc and alsnum are included in the {\bf src} directory. Versions +for Sun (acc compiler .sun), Silicon Graphics (.sgi), DEC OSF/1 (.osf) +are included. + +There is a utility csh script called BUILD. Simply type ./BUILD sun to +compile alscript on the Sun, ./BUILD sgi for Silicon Graphics or BUILD +gcc for use with gcc compiler. See instructions in the file BUILD. +The BUILD script will create a /bin directory and subdirecotry +if not already present. You can create makefiles for different computers +and the BUILD script should still function. + + +\subsection{VAX/VMS Installation} +\label{app6} + +The standard VAX C compiler is not ANSI. Accordingly, ALSCRIPT will require +changes to the source code to compile on a VAX. + +The DEC C++ compiler works OK for alscript. Alscript will also compile +on Dec ALPHA under OpenVMS. A descrip.mms file is included for this +purpose. + +{\em WARNING: I've not tested Version 2.0 of ALSCRIPT on VMS} + +\subsection{Alternative ways of invoking ALSCRIPT} +\label{app7} + +The documentation above describes the interactive mode of running ALSCRIPT. +However, it may be more convenient to run the program as a pipe under +Unix or MS-DOS. Examples are shown here. + +ALSCRIPT is a program for producing pretty versions of multiple +sequence aligments. ALSCRIPT will also format single sequences. A +full description of the program is given in the file "alscript.doc". + +Ways of running alscript: + +\begin{enumerate} + +\item + Interactive mode: just type alscript. + You will be prompted for a command file name. The command file will + define the AMPS blocfile, and name of the file to store the PostScript + output - see alscript.doc for details. + +\item + alscript $<$command\_file$>$\ has same effect as 1, But does not prompt for + the command file + e.g. alscript example1.als + +\item + alscript -q $<$ $<$blocfile$>$ $>$ $<$PostScript$>$ + Quick mode - uses default commands, reads alignment from stdin, + writes PostScript to stdout. This mode creates a command file + called ALPSQ.COM. + + e.g. alscript -q $<$ example1.blc $>$ example1.ps + +\item + alscript -f $<$command\_file$>$ + Similar effect to 2. + +\item + alscript -f $<$command\_file$>$\ -s + Silent operation: No messages are written to stderr, unless fatal. + Silent operation may be toggled by the silent\_mode\ command + in the command file. + +\item + alscript -f $<$command\_file$>$\ -p $<$ $<$blocfile$>$ $>$ $<$PostScript$>$ + Make alscript work like a pipe - blocfile is read from stdin, + postscript is written + to stdout. Messages are written to stderr. To supress messages include + the -s flag too + + e.g. alscript -f example1.als -p -s $<$ example1.blc $>$ example1.ps + +\end{enumerate} + +Using alscript as a pipe has the advantage of allowing the blocfile to +be created on the fly by the programs msf2blc or clus2blc. For example +if we have a GCG .msf file called "pileup.msf" we can run alscript with +default shading/fonts and send the results straight to the PostScript +printer "lpr" as follows: + +msf2blc -q $<$pileup.msf | alscript -q -s | lpr + +\subsection{Program Crashes and Known Bugs} +\label{app8} + +We've used ALSCRIPT on Sun Workstations and Silicon Graphics for some +time, with very large alignments and command files with thousands of +commands. All seems to work OK, the program has not crashed on us at +all!! + +However, the command interpreter in ALSCRIPT is very simple and +the program will crash if you give any command the wrong number of +arguments (e.g. leaving out the shade value in a shade\_chars\ command). + +If you do make the program crash, have checked all the documentation +and your numbers, and the program still crashes. Then send me +the command file and block file that causes the crash and I will try +to investigate. + +Suggestions for improvements to the program are always welcome. + +\subsection{Wish List for next version!!} +\label{app9} + +A command interpreter that does more error checking will be included. +Currently, no checking is done to make sure that the correct number of +arguments are given to a command. + +Sequences will be able to be given unique labels and region commands refer +to these labels or ranges of labels. This will permit a sequence to be +deleted or added to the alignment without having to update the .als file. + +The relative numbering option will be extended to allow numbering relative +to a position. e.g. 456+7 would be 7 residues after position 456. This +will allow annotation of positions that may be in insertions relative to the +reference sequence. + +Special TEXT commands will be extended to allow alternative shapes to +be drawn and scaled in various ways. + +Tree drawing and generalised graphics. An option to draw arbitrary lines +on an alignment will be added. This will permit line graphics to be added +to an alignment. The initial reason for this will be to show dendrograms +(trees) alongside the alignment, but simple line graphs could also be plotted +under the alignment. + +Fiddle factors will be introduced to allow fine positioning of +individual characters. For example, if you like your ``I'' characters +to be centred rather than left justified, this will be possible. + +In single\_page mode, it will be possible to add arbitrary text to an +alignment for final annotation, e.g. titles etc. + +Variable height/width sequence lines will be permitted (maybe). + +\subsection{Acknowledgements} + +I thank all those who have emailed me with suggestions for +improvements to alscript. I've tried to include some of these in the +current distribution (e.g. screening). + +\subsection{References} +\label{app10} + +\begin{verbatim} + +1. Barton, G. J. (1993), + "ALSCRIPT A tool to format multiple sequence alignments", + Protein Engineering, Volume 6, No. 1, pp.37-40. + +2. Barton, G. J. (1990), + "Protein Multiple Sequence Alignment and Flexible Pattern Matching", + Methods in Enzymology, + 183,403-428. + +3. Barton, G. J. and Sternberg, M. J. E. (1987), + "A Strategy for the Rapid Multiple Alignment of Protein Sequences: + Confidence Levels From Tertiary Structure Comparisons", + Journal of Molecular Biology, + 198,327-337 + +4. Higgins, D. G. and Sharp, P. M. (1989), + "Fast and sensitive multiple sequence alignments on a microcomputer", + CABIOS, + 5,151--153 + +5. Devereux, J. Haeberli, P. Smithies, O. (1984), + "A comprehensive set of sequence analysis programs for the VAX", + Nucleic Acids Res. + 12, 387-395 + +6. Livingstone, C. D. and Barton, G. J. (1993), + "Protein Sequence Alignments: A Strategy for the Hierarchical analysis + of residue conservation" + Computer Applications in the Biosciences, + 9, 745-756. + +\end{verbatim} +\end{document} + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/doc/alscript/_19723_tex2html_wrap672.xbm b/sources/alscript/doc/alscript/_19723_tex2html_wrap672.xbm new file mode 100644 index 0000000..27d395d --- /dev/null +++ b/sources/alscript/doc/alscript/_19723_tex2html_wrap672.xbm @@ -0,0 +1,8 @@ +#define noname_width 37 +#define noname_height 15 +static char noname_bits[] = { + 0x86,0x99,0x0c,0x70,0x0c,0x86,0x89,0x08,0x60,0x04,0x86,0x83,0x00,0xc0,0x02, + 0x46,0x83,0x00,0xc0,0x03,0x46,0x82,0x00,0x80,0x01,0xe6,0x87,0x38,0x82,0x03, + 0x26,0x86,0x18,0x40,0x07,0x36,0x8d,0x18,0x60,0x06,0x86,0x81,0x18,0x22,0x0e, + 0xff,0xe0,0xfb,0x3b,0x1e,0x00,0x00,0x18,0x02,0x00,0x00,0x00,0x18,0x00,0x00, + 0x00,0x00,0x18,0x04,0x00,0x00,0x00,0x18,0x06,0x00,0x00,0x00,0xfc,0x03,0x00}; diff --git a/sources/alscript/doc/alscript/alscript.html b/sources/alscript/doc/alscript/alscript.html new file mode 100644 index 0000000..19b4410 --- /dev/null +++ b/sources/alscript/doc/alscript/alscript.html @@ -0,0 +1,80 @@ + +No Title + +

+


+ Next: Contents +

+

+ +

+User Guide to +

+ALSCRIPT - Sequence alignment to PostScript
Version 2.03
 +

+Geoffrey J. Barton +

+Laboratory of Molecular Biophysics
University of Oxford
Rex Richards Building
South Parks Road
Oxford OX1 3QU
U.K.
+

+Tel: (44) 1865-275368
Fax: (44) 1865-510454
e-mail: gjb@bioch.ox.ac.uk +

+REFERENCE:
Barton, G. J. (1993),
ALSCRIPT a tool to format multiple sequence alignments
Protein Engineering, Volume 6, No. 1, pp.37-40.
+

+

+ +
+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/images.log b/sources/alscript/doc/alscript/images.log new file mode 100644 index 0000000..b5a4542 --- /dev/null +++ b/sources/alscript/doc/alscript/images.log @@ -0,0 +1,42 @@ +This is TeX, C Version 3.141 (format=lplain 92.9.20) 24 MAY 1995 16:40 +**19723_images.tex +(19723_images.tex +LaTeX Version 2.09 <18 March 1992> + +(/usr/local/lib/tex/macros/article.sty +Standard Document Style `article' <14 Jan 92>. +(/usr/local/lib/tex/macros/art12.sty) +\c@part=\count79 +\c@section=\count80 +\c@subsection=\count81 +\c@subsubsection=\count82 +\c@paragraph=\count83 +\c@subparagraph=\count84 +\c@figure=\count85 +\c@table=\count86 +) (/usr/local/lib/tex/macros/times.sty +File: `times.sty' PSNFSS 2.3 <12.04.92> (SPQR) +(/usr/local/lib/tex/macros/psnfss.sup +psnfss.sup, PSNFSS 2.2.5<18.8.92> (SPQR) +PostScript additions to NFSS +\driver@setup=\count87 +) +\aadimen=\dimen99 +\aadimen=\dimen100 +\aadimen=\dimen101 +) (/home/nmra/barton/latex2html/html.sty) +No file 19723_images.aux. +[1 + + +] (19723_images.aux) ) +Here is how much of TeX's memory you used: + 309 strings out of 11814 + 7080 string characters out of 80910 + 29464 words of memory out of 262141 + 2419 multiletter control sequences out of 9500 + 19903 words of font info for 74 fonts, out of 100000 for 255 + 0 hyphenation exceptions out of 607 + 16i,4n,22p,352b,76s stack positions out of 300i,40n,60p,3000b,4000s + +Output written on 19723_images.dvi (1 page, 280 bytes). diff --git a/sources/alscript/doc/alscript/images.pl b/sources/alscript/doc/alscript/images.pl new file mode 100644 index 0000000..3752ec1 --- /dev/null +++ b/sources/alscript/doc/alscript/images.pl @@ -0,0 +1,7 @@ +# LaTeX2HTML 0.5.3 (Wed Jan 26 1994) +# Associate image original text (scrambled) with physical files. + +$key = q/{}LaTeX{}/; +$cached_env_img{$key} =''; + +1; \ No newline at end of file diff --git a/sources/alscript/doc/alscript/images.tex b/sources/alscript/doc/alscript/images.tex new file mode 100644 index 0000000..e2e6bd2 --- /dev/null +++ b/sources/alscript/doc/alscript/images.tex @@ -0,0 +1,54 @@ +\batchmode +\documentstyle[12pt,times,html]{article} +\makeatletter + +\makeatother +\newenvironment{tex2html_wrap}{}{} +\setlength{\textheight}{10in}\begin{document} +\pagestyle{empty} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{section} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{section} +\stepcounter{section} +\stepcounter{subsection} +\stepcounter{section} +\stepcounter{section} +\stepcounter{section} +\stepcounter{subsection} +\stepcounter{subsubsection} +\stepcounter{subsubsection} +\stepcounter{subsubsection} +\stepcounter{subsubsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\newpage + +{\samepage \clearpage \LaTeX +} + + +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{subsection} +\stepcounter{section} + +\end{document} \ No newline at end of file diff --git a/sources/alscript/doc/alscript/labels.pl b/sources/alscript/doc/alscript/labels.pl new file mode 100644 index 0000000..dc4dfa8 --- /dev/null +++ b/sources/alscript/doc/alscript/labels.pl @@ -0,0 +1,16 @@ +# LaTeX2HTML 0.5.3 (Wed Jan 26 1994) +# Associate symbolic labels with physical files. + +$external_labels{"app9"} ="$URL/subsection3_16_10.html"; +$external_labels{"new2"} ="$URL/section3_10.html"; +$external_labels{"app2"} ="$URL/subsection3_16_2.html"; +$external_labels{"app5"} ="$URL/subsection3_16_6.html"; +$external_labels{"app8"} ="$URL/subsection3_16_9.html"; +$external_labels{"app10"} ="$URL/subsection3_16_12.html"; +$external_labels{"app1"} ="$URL/subsection3_16_1.html"; +$external_labels{"app4"} ="$URL/subsection3_16_4.html"; +$external_labels{"app7"} ="$URL/subsection3_16_8.html"; +$external_labels{"app3"} ="$URL/subsection3_16_3.html"; +$external_labels{"app6"} ="$URL/subsection3_16_7.html"; + +1; \ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_10.html b/sources/alscript/doc/alscript/section3_10.html new file mode 100644 index 0000000..23468af --- /dev/null +++ b/sources/alscript/doc/alscript/section3_10.html @@ -0,0 +1,35 @@ + + New Features in Version 2.0 + +

+


+ Next: New Step 1 +Up: No Title + Previous: New Features in +

+

New Features in Version 2.0

+

+ +

+Error checking is now done on all ranges input. If you run ALSCRIPT +2.0 on a file that worked with ALSCRIPT 1.4.5, and it complains about +out of range numbers, then check your ranges carefully. If you think +you are right, then send me a minimal example of the problem and I +will investigate. Versions of ALSCRIPT before 2.0 would often work +happily with out of range numbers and produce perfectly OK output. +

+The files ipns.als and ipns.blc show example command and +block file that use most of these new commands. See the examples directory. +

+

+ +
+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_11.html b/sources/alscript/doc/alscript/section3_11.html new file mode 100644 index 0000000..5b7dd9f --- /dev/null +++ b/sources/alscript/doc/alscript/section3_11.html @@ -0,0 +1,22 @@ + + Running ALSCRIPT + +

+


+ Next: Basic Use +Up: No Title + Previous: New special TEXT +

+

Running ALSCRIPT

+

+

+ +
+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_12.html b/sources/alscript/doc/alscript/section3_12.html new file mode 100644 index 0000000..ae63026 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_12.html @@ -0,0 +1,45 @@ + + Using Colour + +

+


+ Next: The MASK command +Up: No Title + Previous: More complex effects +

+

Using Colour

+

+Version 1.4 includes commands to allow the independent colouring of +characters, or their backgrounds. Colours are defined in a similar +manner to fonts using the DEFINE_COLOUR command (American spelling also +allowed). For example: +

+DEFINE_COLOUR 7 1 0 0 +

+defines colour number 7 to be red - see +ALSCRIPT Command Summary +for full details of +this command. Colours 99 and 100 are pre-defined to white and black. +ALSCRIPT assumes the paper colour is white. +

+The command to colour the text of a character or text string is: +

+CCOL_CHARS +

+the command to colour the background of a character is +

+SCOL_CHARS +

+both have similar syntax to the FONT_CHARScommand. +

+COLOUR_REGION and COLOUR_RES have similar syntax to SHADE_REGION and +SHADE_RES. +

+An example command file that uses colour is shown in example3.als. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_13.html b/sources/alscript/doc/alscript/section3_13.html new file mode 100644 index 0000000..8d77a5e --- /dev/null +++ b/sources/alscript/doc/alscript/section3_13.html @@ -0,0 +1,65 @@ + + The MASK command family + +

+


+ Next: Summary of mask +Up: No Title + Previous: Using Colour +

+

The MASK command family

+

+The idea behind the MASK command is to build up a set of character +positions that will subsequently be boxed, shaded, set in a particular +font, etc. For example, lets say we want to box the most frequently +occuring character at each position in an alignment. +

+The command +

+mask SETUP +

+tells ALSCRIPT to prepare a mask. +

+mask FRE ALL +

+specifies that the most frequently occuring character at each position +in the alignment will be masked. This command can be restricted to a +region of the alignment using: mask FRE sx sy ex ey, where sx etc +define the region in the same way as for font_region and other commands. +

+mask BOX ALL +

+Tells ALSCRIPT to create the boxing lines that will separate the masked +characters from non-masked characters - this command may also be +restricted to a region of the alignment. +

+The mask can be reset for re-use using the command: +

+mask RESET +

+Two further commands define which characters can be used when +calculating the mask. This allows gap-characters, or other amino acids +to be excluded from the calculation to avoid unwanted boxing. +

+mask LEGAL "AVLI" +

+defines the AVL and I as the only characters that will be used when +calculating the mask. +

+mask ILLEGAL ".-_" +

+defines .- and _ as characters that will not be used when calculating +the mask. NOTE: the blank character " " cannot be defined in this way. +To avoid boxing " " characters substitute blanks for something else +(using SUB_CHARS), calculate the mask, then substitute back. +

+

+ +
+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_14.html b/sources/alscript/doc/alscript/section3_14.html new file mode 100644 index 0000000..c346ef7 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_14.html @@ -0,0 +1,33 @@ + + Printing ALSCRIPT Files + +

+


+ Next: Conclusion +Up: No Title + Previous: Summary of mask +

+

Printing ALSCRIPT Files

+

+ALSCRIPT produces files in PostScript which may be printed on a PostScript +printer (e.g. an Apple LaserWriter). If you don't have a PostScript printer, +then you may still be able to use ALSCRIPT if you get hold of the +GhostScript software. This is a free package that interprets PostScript +commands and can produce output on a large number of different types of +printer. GhostScript runs on most hardware types (including PCs) and can also +display output to the screen. The package can be obtained from many different +sites on the Internet (In the UK try src.doc.ic.ac.uk). +

+The actual command you need to type to send a PostScript file to the printer +will depend on your system. Consult your system manager for help. +

+Be warned, ALSCRIPT can create extremely large PostScript files if +lots of boxing and shading is done on big alignments. On older +printers such output may take a long time to process. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_15.html b/sources/alscript/doc/alscript/section3_15.html new file mode 100644 index 0000000..1dbd769 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_15.html @@ -0,0 +1,28 @@ + + Conclusion + +

+


+ Next: Appendices +Up: No Title + Previous: Printing ALSCRIPT Files +

+

Conclusion

+

+ALSCRIPT provides a powerful set of formatting and editing commands +specifically tailored for multiple sequence alignments. It is best +used in conjunction with a PostScript previewer such as Sun's +PageView or GhostView since this allows the effect of changing a command to be +seen quickly. In the absence of such a tool, simpler effects can be +tested out without destroying too many trees in the Laser Printer! +

+Like most programs, ALSCRIPT is evolving as I find new problems to display, so +if you have any suggestions - I shall endeavour to include them in a later +version. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_16.html b/sources/alscript/doc/alscript/section3_16.html new file mode 100644 index 0000000..c43f3b8 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_16.html @@ -0,0 +1,38 @@ + + Appendices + +

+


+ Next: ALSCRIPT Command Summary +Up: No Title + Previous: Conclusion +

+

Appendices

+

+

+ +
+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_17.html b/sources/alscript/doc/alscript/section3_17.html new file mode 100644 index 0000000..3748952 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_17.html @@ -0,0 +1,17 @@ + + <em>About this document ...</em> + +

+


+Up: No Title + Previous: References +

+

About this document ...

+

+ This document was generated using the LaTeX2HTML translator Version 0.5.3 (Wed Jan 26 1994) Copyright © 1993, Nikos Drakos, Computer Based Learning Unit, University of Leeds.

The command line arguments were:
+latex2html -address gjb@bioch.ox.ac.uk alscript.tex.

The translation was initiated by gjb@ on Wed Jun 5 16:43:14 BST 1996

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_2.html b/sources/alscript/doc/alscript/section3_2.html new file mode 100644 index 0000000..f9d52c9 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_2.html @@ -0,0 +1,44 @@ + + Update History + +

+


+ Next: Read This First +Up: No Title + Previous: Contents +

+

Update History

+

+


+
+VERSION 1.0   19 June     1992
+Version 1.1   26 June     1992
+Version 1.2   21 October  1992: Add multiple blocks per page option.
+Version 1.3   15 November 1992: First Distribution.
+Version 1.4    6 December 1992: Add Colour commands.
+Version 1.4.1  1 February 1993: Small bug fixes - FULL RELEASE VERSION.
+Version 1.4.2 15 February 1993: Make silent_mode toggle.
+Version 1.4.3  1 March    1993: Fix bug in colour option.
+Version 1.4.4 24 March    1993: Add mask features (should be version 1.5).
+Version 1.4.5 25 May      1993: Include alsnum program in distribution.
+	       7 June     1993: Fix NO_NUMBERS bug in documentation
+                                Change defaults for -q option to use MASK.
+
+Version 2.0   23 May 	  1995: Numerous changes and additions including the
+				option to colour backgrounds
+				differently, ommission of idents on
+				second and subsequent lines, helix,
+				strand and other special characters,
+				relative numbering, error checking of
+				ranges on input, bounding box,
+				screening, conservation colouring...
+Version 2.03   5 June     1996: Small bug fixes - patches incorporated.
+				BACKGROUND_REGION and BOUNDING_BOX commands moved to 
+				step 1 section.
+

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_3.html b/sources/alscript/doc/alscript/section3_3.html new file mode 100644 index 0000000..4af8000 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_3.html @@ -0,0 +1,26 @@ + + Read This First - VERSION 2.0 + +

+


+ Next: Related Programs +Up: No Title + Previous: Update History +

+

Read This First - VERSION 2.0

+

+This manual describes an interim release of ALSCRIPT that includes +many additional features over the previously distributed Version +1.4.5. I had hoped to make a lot more changes and improvements before +distributing the new version, however I have not had the time to do +this. I am distributing Version 2.0 since the new features have been +used in a number of published alignment figures. Please see +New Features in Version 2.0 for details +of the new features. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_4.html b/sources/alscript/doc/alscript/section3_4.html new file mode 100644 index 0000000..1243c36 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_4.html @@ -0,0 +1,30 @@ + + Related Programs + +

+


+ Next: Availability +Up: No Title + Previous: Read This First +

+

Related Programs

+

+The AMPS package (Barton, 1990). This performs multiple sequence +alignments and databank scanning. +

+AMAS (Livingstone and Barton, 1993, CABIOS, 9, 745-756). Analysis of +Multiply Aligned Sequences. This package uses a sophisticated +set-based method to identify patterns of residue conservation in +multiple sequence alignments. +

+All programs are available by anonymous ftp from geoff.biop.ox.ac.uk. +Please see the README file for details licencing and registration. +You can read manuals for the programs and some related papers at +http://geoff.biop.ox.ac.uk/. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_5.html b/sources/alscript/doc/alscript/section3_5.html new file mode 100644 index 0000000..9c22b0a --- /dev/null +++ b/sources/alscript/doc/alscript/section3_5.html @@ -0,0 +1,22 @@ + + Availability + +

+


+ Next: Installing ALSCRIPT +Up: No Title + Previous: Related Programs +

+

Availability

+

+ALSCRIPT is available free of charge for academic and non-commercial +purposes. Distribution is by anonymous ftp from geoff.biop.ox.ac.uk. +See the README file on the ftp server for details. You need to +register with G. J. Barton before downloading the software. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_6.html b/sources/alscript/doc/alscript/section3_6.html new file mode 100644 index 0000000..2280e0e --- /dev/null +++ b/sources/alscript/doc/alscript/section3_6.html @@ -0,0 +1,23 @@ + + Installing ALSCRIPT + +

+


+ Next: Brief Description of +Up: No Title + Previous: Availability +

+

Installing ALSCRIPT

+

+See the appropriate section for the computer type you are using: +

+For PCs see 386 MS-DOS installation. +For Unix see Unix installation. +For VMS see VMS installation. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_7.html b/sources/alscript/doc/alscript/section3_7.html new file mode 100644 index 0000000..51412a1 --- /dev/null +++ b/sources/alscript/doc/alscript/section3_7.html @@ -0,0 +1,83 @@ + + Brief Description of ALSCRIPT + +

+


+ Next: New Features in +Up: No Title + Previous: Installing ALSCRIPT +

+

Brief Description of ALSCRIPT

+

+ALSCRIPT takes a multiple sequence alignment in AMPS (Barton &Sternberg, 1987, Barton, 1990) block-file format and a set of +formatting commands and produces a PostScript file that may be printed +on a PostScript laser printer, or viewed using a PostScript previewer +(e.g. Sun Microsystem's PageView program). CLUSTAL and GCG format +multiple alignment files may also be used (see below). ALSCRIPT is NOT a +multiple sequence alignment program, nor is it an alignment editor. +

+Given a block-file and pointsize (character width/height), ALSCRIPT +calculates how many residues can be fitted across the page, and how +many sequences will fit down the page, it then prints the alignment at +the chosen pointsize on as many pages as are needed. Running ALSCRIPT with +a smaller or larger pointsize will automatically re-scale the alignment +to fit on fewer or more pages as appropriate. The actual page +dimensions may be re-set to any value, so if you have access to an A3 +PostScript printer, or typesetting machine, alignments can readily be +scaled to maximise the available space. +

+Each output page has three basic regions. The left hand edge contains +identifier codes for each sequence. The main part of the page holds the +alignment, and the top part, the position numbers and tick marks. ALSCRIPT +commands make use of a character coordinate system for font changes, +and other formatting commands. Thus, any residue in the alignment may +be referred to by its sequence position number (x-axis) and sequence +number (y-axis), similarly, ranges of residue positions, or sequences +may also be defined in the character coordinate system. +

+The basic ALSCRIPT commands allow the following functionality: +

+Fonts: Any PostScript font at any size may be defined and used on +individual residues, regions or identifier codes. +

+Boxing: Simple rectangular boxes may be drawn around any part of the +alignment. Particular residue types may be selected and automatically +"surrounded" by lines. For example, if the characters 'G' and 'P' are +selected, then lines will not be drawn between G and P characters, but +only where G and P border with other characters. +

+Shading: Grey shading of any level from black to white may be applied +to any region of the alignment, either as a rectangular region, or as +residue specific shading. e.g. "shade all Cys residues between +positions 6 and 30" +

+Text: Specific text strings may be added to the alignment at any +position and in any font or font size. +

+Lines: Horizontal or vertical lines may be drawn to the left, right, top +or bottom of any residue position or group of positions. +

+Colour: Characters or character backgrounds may be independently +coloured. +

+The example block file "example1.blc" and command file "example1.als" +illustrate most of these commands in action. +

+Although written with the aim of producing figures for journal +submission, ALSCRIPT may be used as a tool for interpreting multiple +sequence alignments. For example, the boxing, shading and font changing +facilities can be applied to highlight amino acids of a particular type +and thus draw attention to clusters of positive or negative charge, +hydrophobics, etc. +

+


+ Next: New Features in +Up: No Title + Previous: Installing ALSCRIPT +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_8.html b/sources/alscript/doc/alscript/section3_8.html new file mode 100644 index 0000000..aec717a --- /dev/null +++ b/sources/alscript/doc/alscript/section3_8.html @@ -0,0 +1,38 @@ + + New Features in Version 1.4.4 + +

+


+ Next: New Features in +Up: No Title + Previous: Brief Description of +

+

New Features in Version 1.4.4

+

+This version introduces the MASK family of commands which allows +boxing, shading etc to be applied according to the frequency of +occurence of the character types at each position in the alignment. +For example, it is possible to box positions where one character is +seen in more than N of the sequences. It is also possible to +box/shade etc the most frequently occurring character at each +position. Commands exist to select which characters will be used in +the calculation of frequencies and which will be excluded, thus boxing +can be based upon two or more character types at a position. MASK +commands also exist to show residues identical to one sequence in the +set. See the section on MASK below for details. +

+NOTE: Although boxing according to the frequency of amino acids seen +at a position is a popular method of representation it is not usually +the most informative. An analysis that takes into account the +physico-chemical properties of the amino acids and also relates the +amino acid similarities to the overall similarity between the +sequences is more helpful in identifying functionally important +residues. The AMAS program (Livingstone and Barton, 1993) applies a +flexible hierarchical set-based approach to this problem. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/section3_9.html b/sources/alscript/doc/alscript/section3_9.html new file mode 100644 index 0000000..68ee99e --- /dev/null +++ b/sources/alscript/doc/alscript/section3_9.html @@ -0,0 +1,50 @@ + + New Features in Version 1.4.5 - Program alsnum + +

+


+ Next: New Features in +Up: No Title + Previous: New Features in +

+

New Features in Version 1.4.5 - Program alsnum

+

+Version 1.4.5 includes the program "alsnum". This is a temporary solution to +the residue numbering problem. Ultimately, these functions will be included +as alscript commands. +

+alsnum creates a set of TEXT commands that can be incorporated into an +alscript command file to place sequential numbers at any position in the +alignment. The numbers ignore gaps, so the numbering will correspond to +the specific sequence position rather than the alignment. +

+To use the program: +

+1. Decide where you want the numbers to be placed. For example, you might +want the numbers above the third sequence in the alignment. If so, make +an extra sequence space above the third sequence using the ADD_SEQ command. +

+2. Decide what is the number of the first residue of the sequence to be +numbered. This will not always be 1 since you may be aligning fragments +or domains. +

+3. Decide the numbering interval (e.g. every 10th amino acid). +

+4. Run the program. +

+For example, if you want to add numbers according to sequence 37 of a +block file (junk.blc), calling the first residue of the sequence 12, +and with an interval of 5, and the numbers are to be placed at the +location of sequence 3 in the alignment. Type: +

+alsnum 37 12 5 3 < junk.blc > junk.text +

+5. Add the resulting TEXT commands from junk.text to your alscript +command file. +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/subsection3_10_1.html b/sources/alscript/doc/alscript/subsection3_10_1.html new file mode 100644 index 0000000..a9cc28f --- /dev/null +++ b/sources/alscript/doc/alscript/subsection3_10_1.html @@ -0,0 +1,74 @@ + + New Step 1 Commands + +

+


+ Next: New Step 2 +Up: New Features in + Previous: New Features in +

+

New Step 1 Commands

+

+SCREENSIZE 120 +

+Usually you should not need to change this value, it alters the +screening used by the printer. A value of 120 is used by default. On +most 300dpi black and white printers this gives much smoother greys +than the default used in earlier versions of ALSCRIPT. +

+PIR_SAVE filename +

+Will cause the block file to be saved into the file ``filename'' in +PIR format. This can be useful for moving block file alignments to other +programs. +

+MSF_SAVE filename +

+Will cause the block file to be saved into the file ``filename'' in +something that approximates GCG .msf format. Warning! This has not +been fully tested. +

+NUMBER_COLOUR 4 +

+Sets the colour used for numbering at the top of the alignment (no +American spelling at the moment). In this example, colour number 4 has been +defined (See the DEFINE_COLOURcommand if you are not sure what this means). +

+SINGLE_PAGEIf this is set, ALSCRIPT assumes everything will be plotted on one +page. At the moment, all this does is write the bounding box for the +figure, so encapsulating the PostScript. This may allow the +output of alscript to be imported into word processors etc, but +probably not all. +

+ID_ONLY_ON_FIRST_LINEIf this is present, then sequence identifiers will only be printed on +the first line of the alignment. Often this looks better for small +alignments than the default. +

+BACKGROUND_COLOUR 7 +

+Sets the colour used for the background to the alignment. This can be +useful for preparing figures for projection. At the moment this only +works reliably when the SINGLE_PAGEis also set. +

+BOUNDING_BOX x y x1 y1 +

+Defines the bounding box for the figure. This is set in points (1/72 inch). +NOTE: In version 2.0 this was a STEP 2 Command. +

+BACKGROUND_REGION x y x1 y1 +

+Defines the region to colour as background - the default is set up for A4 paper +so US users may have to fiddle with this. Values are points (1/72 inch). +NOTE: In version 2.0 this was a STEP 2 Command. +

+


+ Next: New Step 2 +Up: New Features in + Previous: New Features in +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/subsection3_10_2.html b/sources/alscript/doc/alscript/subsection3_10_2.html new file mode 100644 index 0000000..e9682cc --- /dev/null +++ b/sources/alscript/doc/alscript/subsection3_10_2.html @@ -0,0 +1,67 @@ + + New Step 2 Commands + +

+


+ Next: New special TEXT +Up: New Features in + Previous: New Step 1 +

+

New Step 2 Commands

+

+COLOUR_TEXT_REGION x y x1 y1 colour +

+Sets the colour for TEXT command output. Similar syntax to COLOUR_REGION, FONT_REGIONetc. +

+COLOUR_LINE_REGION x y x1 y1 colour +

+Set the colour for LINEs in a region. +

+CALCONS x y x1 y1 +

+Calculate conservation values according to Zvelebil et al. for the designated +region. (See Livingstone &Barton 1993 for details and further refs) +

+MASK CONSERVATION cutoff +

+If CONSCAL has been used, then mask residues according to the conservation cutoff. +

+e.g. MASK CONSERVATION 10 would mask all identities, MASK CONSERVATION 6 would +mask reasonably conserved positions. See examples for more on this command. +

+HELIX x1 y x2 +

+Draw a helix from x1 to x2 of sequence y. +

+STRAND x1 y x2 +

+Draw a strand from x1 to x2 of sequence y. +

+COIL x1 y x2 +

+Draw a coil (horizontal line) from x1 to x2 of sequence y. +

+RELATIVE_TO<seqnum> <startnum> +

+Set reference numbers to work relative to sequence number <seqnum>. This +means that in all subsequent commands, ALSCRIPT will translate your x +values into absolute position values in the alignment. This is extremely useful since you can annotate your alignment using your +favourite sequences as a reference point. You no longer have to +translate every x position into the alignment position. +

+<startnum> is optional. If present, it specifies what the first +residue in the displayed sequence is. For example, you may be showing +residues 200-500 of a sequence, so <startnum> would be 200 rather than +the default of 1. Warning - this is a very new feature and +bounds checking is not fully enabled for it. +

+You can use RELATIVE_TOseveral times in the command file to annotate +different sequences. RELATIVE_TO0 resets to the ``normal'' +alignment numbering. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Running ALSCRIPT +Up: New Features in + Previous: New Step 2 +

+

New special TEXT commands

+

+Some special TEXT commands have been added to allow drawing of alternative +shapes etc. In fact this is how the HELIX, STRAND and COIL commands are implemented. The text commands are all prefixed by an @ symbol. +

+e.g. TEXT 3 6 ``@fuparrow'' +

+will draw a filled up arrow at position 3,6. +

+The alternative text commands are: +

+@leftarrow - an open left pointing arrow. +

+@fleftarrow - a filled left pointing arrow. +

+@uparrow - an open up pointing arrow. +

+@fuparrow - a filled up pointing arrow. +

+@downarrow - an open down pointing arrow. +

+@fdownarrow - a filled down pointing arrow. +

+@circle - an open circle. +

+@fcircle - a filled circle. +

+I plan to make this option more flexible in the near future. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: More complex effects +Up: Running ALSCRIPT + Previous: Running ALSCRIPT +

+

Basic Use

+

+I recommend you read through this section, then scan the commands in +ALSCRIPT Command Summary to get a feel for what ALSCRIPT can do. +

+See Alternative ways of invoking ALSCRIPT. In this section, the +interactive method is described. The QUICK START method shown in +Alternative ways of invoking ALSCRIPT +is useful to format a sequence alignment quickly +using standard pointsize and shading. +

+ALSCRIPT is designed to work with AMPS block file format multiple +alignments. If you have a multiple alignment generated by CLUSTAL V +or the GCG package, then it must be translated to AMPS block file +format. +

+To translate a GCG .MSF file: Type: msf2blc. +To translate a CLUSTAL PIR format file, or any PIR format file: clus2blc. +

+Both programs prompt for the name of an input file, and an output +block file name. A good convention to follow is to name all blockfiles +with the extension ".blc". +

+To run ALSCRIPT simply type: +

+alscript +

+you will then be prompted for the name of the ALSCRIPT command file. +Having typed the filename, the commands will be executed as you have +specified. +

+A Simple Command File (example.als) +

+The file example.blc contains a small multiple sequence alignment. +The following ALSCRIPT command file will convert this into a +PostScript alignment file in 12 point Helvetica. +

+


+#Comments in ALSCRIPT command files start with a #
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE 	example.blc 		#define the block file to format
+OUTPUT_FILE 	example.ps		#where to put the result
+LANDSCAPE				#landscape paper orientation
+POINTSIZE 12				#12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT		#set font 0 to be Helvetica
+SETUP					#Tell the program to get on with it.
+

+Now try changing the POINTSIZE value to 5 ALSCRIPT will re-format the +alignment to make best use of the available paper. +

+These are all STEP 1 commands - they refer to overall layout, and system +settings - for example, the paper size or maximum sequence length. +Other commonly used STEP 1 commands are IDENT_WIDTH which reserves more +or less width for the sequence identifier codes, NUMBER_SEQS which adds +a number to each sequence and LINE_WIDTH_FACTOR which allows the +thickness of all boxing lines to be adjusted. See +ALSCRIPT Command Summary +for details of these and all other STEP 1 commands. +

+The simple example outlined above can be modified with a variety of +STEP 2 commands. +

+for example file example2.als: +

+


+# FILE example2.als
+#
+#Commands are free format - separated by blank, tab or comma characters
+#
+BLOCK_FILE 	example.blc 		#define the block file to format
+OUTPUT_FILE 	example2.ps		#where to put the result
+LANDSCAPE				#landscape paper orientation
+POINTSIZE 12				#12 point default pointsize
+DEFINE_FONT 0 Helvetica DEFAULT		#set font 0 to be Helvetica
+DEFINE_FONT 1 Helvetica REL 0.5         #set font 1 to be half sized Helvetica
+DEFINE_FONT 3 Helvetica-Bold DEFAULT    #set font 3 to be Bold Helvetica
+DEFINE_FONT 4 Times-BoldItalic DEFAULT  #font 4 is Times-BoldItalic
+NUMBER_SEQS				#Number the sequences at left hand side
+SETUP					#Tell the program to get on with it.
+#
+#step 2 commands come after the SETUP command 
+#
+#Here are some examples...
+#
+SURROUND_CHARS GP ALL		#draw lines around all G and P
+SHADE_CHARS ILVW ALL 0.6	#shade all I L V and W with value 0.6
+BOX_REGION 1 1 2 20 0.8		#rectangular box from positions 1 to 2 of sequences 1 to 20
+FONT_CHARS C ALL 3              #Use font 3 (BOLD Helvetica) for C characters
+ID_FONT ALL 1                   #set identifiers in font 1
+

+There are many possible ways of combining these commands and the +others shown in +ALSCRIPT Command Summary. +In general, if you apply multiple +commands to the same residue, the effect of the last applied command +persists where there would otherwise be a conflict. Thus the +intersection of two overlapping SHADed regions would be shaded +according to the second SHADE command, not some mixture of the two. +Similarly for FONT commands. BOX and SURROUND commands behave in the +opposite sense, all BOXing and SURROUNDing persists regardless of how +many commands you issue. This makes it possible for example, to +SURROUND two different sets of residues as follows: +

+


+
+SURROUND_CHARS DE ALL
+SURROUND_CHARS DEHKR ALL
+

+This would result in D and E characters being partitioned from the rest as well +as D E H K R characters (see Example output). +

+


+ Next: More complex effects +Up: Running ALSCRIPT + Previous: Running ALSCRIPT +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/subsection3_11_2.html b/sources/alscript/doc/alscript/subsection3_11_2.html new file mode 100644 index 0000000..4b6251f --- /dev/null +++ b/sources/alscript/doc/alscript/subsection3_11_2.html @@ -0,0 +1,92 @@ + + More complex effects - Text Lines, and Masks + +

+


+ Next: Using Colour +Up: Running ALSCRIPT + Previous: Basic Use +

+

More complex effects - Text Lines, and Masks

+

+Text, lines and masking are meant to be used to annotate the multiple +alignment. The TEXT command allows any piece of text to be located +anywhere on the alignment. Clearly, however it makes little sense to +superimpose the text over the alignment though this can be done! Accordingly, +you must first make a space to put the text in. Usually, this will be a +few lines below the multiple sequence alignment, but you may want to +add text at the top, or somewhere in between two sequences. You can make +space in two ways. Either by editing the block-file to introduce "dummy" +sequences at the locations you want, or by making use of the ADD_SEQ +command. +

+The ADD_SEQ command has two arguments, the sequence after which you want +further sequences to be added, and how many blank sequences you need. +Thus, we can reserve space for 5 lines of text underneath a 10 sequence +multiple alignment with the following command. +

+ADD_SEQ 10 5 +

+we can then put text below the alignment at the 20th residue. +

+TEXT 20 13 "Active Site His" +

+or any other position. +

+Similarly, we could draw a vertical line to point out which residue we mean +

+LINE LEFT 20 13 11 +

+And change the font of the text to number 7 (whatever that has been set to): +

+FONT_RESIDUE 20 13 7 +

+You can have multiple ADD_SEQ commands, but they must occur in sequence order. +Thus: +

+


+ADD_SEQ 0 5
+ADD_SEQ 5 12
+is legal. +But +

+ADD_SEQ 5 12
+ADD_SEQ 0 5
+

+Is NOT!! NO CHECKING IS performed by the program for this error - so beware! +

+Note that add_seq commands refer to the actual sequence number as +implied by the block file, not the number after applying the add_seq +command. Thus, for a four sequence block file, if you want to add +space for three sequences before sequence 1 and two sequences after +sequence 3, the commands would be: +

+


+ADD_SEQ 0 3
+ADD_SEQ 3 2
+

+Text added with the TEXT command will not be split across page breaks, +so you may in some circumstances need to fiddle a little with the +location/pointsize for the text to get the desired result. +

+Masking is a technique for drawing irregular shaped outlines, or +shaded regions - this should not be confused with the MASK family of commands +described below. For example a histogram can be added to the bottom of +an alignment by first defining some dummy sequences in the block-file +that have letters building up the shape of the histogram, then using +the SURROUND_CHARS or SHADE_CHARS commands together with the SUB_CHARS +command to produce the desired effect. An example of this operation +being used to show frequencies of secondary structure predictions is +shown in example1.als and in the Protein Engineering paper. +

+


+ Next: Using Colour +Up: Running ALSCRIPT + Previous: Basic Use +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Printing ALSCRIPT Files +Up: The MASK command + Previous: The MASK command +

+

Summary of mask commands

+

+mask SETUP +#Prepares for masking +

+mask LEGAL <qstring> +#defines characters to include in ID or FRE calcs - optional. +

+mask ILLEGAL <qstring> +#defines characters to exclude in ID or FRE calcs - optional. +

+mask ID ALL N +#Calculates a mask that flags the character that occurs at least N +times at a position. The word ALL can be substituted by four numbers +that define a region of the alignment. +

+mask FRE ALL +#calculates a mask that flags the most frequently occuring amino acid +at each position. ALL may be replaced by four numbers defining a region +of the alignment. +

+Multiple mask FRE or mask ID commands may be applied, using different +LEGAL and ILLEGAL character definitions. In this way more complex +effects can be built up. +

+The mask command also allows characters that are identical to one +sequence to be masked. +

+mask AGREE ALL N +

+will mask all positions that are identical to the Nth sequence. Thus, +for sequences that are very similar to a newly sequenced sequence, all +characters identical to the new sequence can be boxed or shaded, or set +in a different font or colour etc... +

+mask NOT ALL +

+allows the mask to be inverted. Thus, all positions that are NOT in the +mask now form the mask. So, having done a mask AGREE, a mask NOT will +allow the positions that are not identical to the selected sequence to +be highlighted or substituted. +

+mask SUB ALL <char> +

+substitutes all characters in the mask with the character <char>. +

+mask REGION ALL +applies a mask to all residues in the defined region. +

+The following effects can now be applied to the masked characters: +

+mask BOX ALL +#boxes the masked residues - ie surrounds them by lines. +

+mask SHADE ALL <grey> +#shades the masked residues by grey value. +

+mask FONT ALL <fontnum> +#Uses font fontnum to output the masked residues. +

+mask INVERSE ALL +#Inverts the masked characters - ie outputs them in white. +

+mask CCOL ALL <colnum> +#outputs the masked characters in the defined colour. +

+mask SCOL ALL <colnum> +#outputs the backgrounds of the masked characters in the defined +colour. +

+In all commands, the word ALL can be replaced by four numbers defining +the region to which the command is applied. +

+mask RESET +#resets the entire mask for re-use +

+


+ Next: Printing ALSCRIPT Files +Up: The MASK command + Previous: The MASK command +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: STEP 1 COMMANDS +Up: Appendices + Previous: Appendices +

+

ALSCRIPT Command Summary

+

+ +

+WARNING: Very little error checking is performed on command input. If +you give the wrong number of arguments to a command, then unexpected +things may happen, or the program will crash very inelegantly. I hope +to fix this in the next version of the program, in the meantime, make sure +you give the correct number of arguments to each command. +

+All commands up to the first space character may be entered in UPPER +or lower case or MiXEd case. Qualifiers for commands (e.g. REL) must +be written in UPPER case. +

+Command Reference: +

+


+<int>  = enter an integer  (e.g. 240)
+<float> = enter a floating point number (e.g. 0.45)
+<string> = enter a string (e.g.  ARNDql)
+<qstring> = enter a quoted string (e.g. "Active Site")
+<char>    = enter a single character.
+

+

+ +
+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Acknowledgements +Up: Appendices + Previous: Program Crashes and +

+

Wish List for next version!!

+

+ +

+A command interpreter that does more error checking will be included. +Currently, no checking is done to make sure that the correct number of +arguments are given to a command. +

+Sequences will be able to be given unique labels and region commands refer +to these labels or ranges of labels. This will permit a sequence to be +deleted or added to the alignment without having to update the .als file. +

+The relative numbering option will be extended to allow numbering relative +to a position. e.g. 456+7 would be 7 residues after position 456. This +will allow annotation of positions that may be in insertions relative to the +reference sequence. +

+Special TEXT commands will be extended to allow alternative shapes to +be drawn and scaled in various ways. +

+Tree drawing and generalised graphics. An option to draw arbitrary lines +on an alignment will be added. This will permit line graphics to be added +to an alignment. The initial reason for this will be to show dendrograms +(trees) alongside the alignment, but simple line graphs could also be plotted +under the alignment. +

+Fiddle factors will be introduced to allow fine positioning of +individual characters. For example, if you like your ``I'' characters +to be centred rather than left justified, this will be possible. +

+In single_pagemode, it will be possible to add arbitrary text to an +alignment for final annotation, e.g. titles etc. +

+Variable height/width sequence lines will be permitted (maybe). +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: References +Up: Appendices + Previous: Wish List for +

+

Acknowledgements

+

+I thank all those who have emailed me with suggestions for +improvements to alscript. I've tried to include some of these in the +current distribution (e.g. screening). +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: About this document +Up: Appendices + Previous: Acknowledgements +

+

References

+

+ +

+


+
+1.  Barton, G. J. (1993), 
+	"ALSCRIPT A tool to format multiple sequence alignments",
+	Protein Engineering,  Volume 6, No. 1, pp.37-40.
+
+2.  Barton, G. J. (1990),
+    	"Protein Multiple Sequence Alignment and Flexible Pattern Matching",
+	Methods in Enzymology,
+    	183,403-428.
+
+3.  Barton, G. J. and Sternberg, M. J. E. (1987),
+    	"A Strategy for the Rapid Multiple Alignment of Protein Sequences: 
+         Confidence Levels From Tertiary Structure Comparisons",
+	Journal of Molecular Biology,
+    	198,327-337
+
+4. Higgins, D. G. and Sharp, P. M. (1989),
+    	"Fast and sensitive multiple sequence alignments on a microcomputer",
+	CABIOS,
+    	5,151--153
+
+5. Devereux, J. Haeberli, P. Smithies, O. (1984),
+    	"A comprehensive set of sequence analysis programs for the VAX",
+	Nucleic Acids Res.
+    	12, 387-395
+
+6. Livingstone, C. D. and Barton, G. J. (1993),
+	"Protein Sequence Alignments:  A Strategy for the Hierarchical analysis
+	of residue conservation"
+	Computer Applications in the Biosciences,
+	9, 745-756.
+
+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: PostScript Fonts +Up: Appendices + Previous: STEP 2 COMMANDS +

+

AMPS Block file format

+

+ +

+The first part of a block-file contains the identifier codes of the +sequences that are to follow. Each code is prefixed by the > symbol, codes +must not contain spaces. +

+e.g. +


+>HAHU
+>Trypsin
+>A0046
+>Seq1
+

+etc. +

+ALSCRIPT counts the number of > symbols in the beginning of the file +until a * symbol is found. The * signals the beginning of the +multiple alignment which is stored VERTICALLY, thus columns are +individual sequences, whilst rows are aligned positions. The * symbol +must lie over the first sequence. A further star in the same column +signals the end of the alignment. ALSCRIPT uses the number of > +symbols at the beginning of the file to work out how many columns to +read from the * position. It is therefore important that the only > +symbols in the file are those that define the identifiers, and the +only * symbols are those defining the start and end of the multiple +alignment. The block file can contain additional text, providing that +there are no more > or * symbols in the file than those used to define +the identifiers or alignment start and end. +

+A simple, small block-file is shown here. +

+


+>Seq_1
+>A0231
+>HAHU
+>Four_Alpha
+>Globin
+>GLobin_C
+*
+ARNDLQ
+AAAAAA
+PPPPPP
+PP PPP
+WW WWW
+LLLLLL
+IIVVLL
+*
+

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: 386 DOS installation +Up: Appendices + Previous: AMPS Block file +

+

PostScript Fonts

+

+ +


+Times-Roman,   
+Times-Italic,  
+Times-Bold,  
+Times-BoldItalic,
+Helvetica,  
+Helvetica-Oblique,  
+Helvetica-Bold,   
+Helvetica-BoldOblique,    
+Courier,    
+Courier-Oblique,    
+Courier-Bold
+Courier-BoldOblique,      
+AvantGarde-Book,       
+AvantGarde-BookOblique,     
+AvantGarde-Demi,    
+AvantGarde-DemiOblique,
+Bookman-Demi,  
+Bookman-DemiItalic,  
+Bookman-Light,  
+Bookman-LightItalic,     
+Helvetica-Narrow,    
+Helvetica-Narrow-Bold,
+Helvetica-Narrow-BoldOblique,      
+Helvetica-Narrow-Oblique,
+NewCenturySchblk-Roman,               
+NewCenturySchlbk-Bold,
+NewCenturySchblk-Italic,        
+NewCenturySchblk-BoldItalic,
+Palatino-Roman,  
+Palatino-Bold,  
+Palatino-Italic,  
+Palatino-BoldItalic 
+ZapfChancery-MediumItalic.
+Symbol
+

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: TECHNICAL NOTES +Up: Appendices + Previous: PostScript Fonts +

+

386 DOS installation

+

+ +

+IMPORTANT - The programs on this disk will ONLY WORK on a PC with a 386 +or better processor. See the Technical Notes section for details of why. +

+Directions: +

+

  1. + Create a directory on your hard disk. + e.g. mkdir ALSCRIPT. +

    +

  2. + Copy the Contents of the floppy disk into this directory. +
    
+    e.g.   copy a:*.* c:\alscript.
    +

    +

  3. + Edit your AUTOEXEC.BAT file and add +
    
+ C:\ALSCRIPT to your path.
    +

    +

  4. + Edit your AUTOEXEC.BAT file and add the following two lines. + set DOS4GVM=@ALSCRIPT.VMC + set DOS4G=quiet +
+

+The first line is an instruction to read instructions from the file +ALSCRIPT.VMC. This sets up a permanent swap file on your hard disk. +By default, the swap file is about 12MBytes in size. If you do not have +this much free space on your disk, then edit the ALSCRIPT.VMC file +to reduce the swap file size, or alternatively, do not put this line +in your autoexec.bat. +

+The programs will run without this swap file, but you will be limited in + the size of alignment you can process by the amount of RAM you have +installed. I have only tested this program on a 486/33 with 8MBytes RAM +and a 386/33 with 4MBytes so I do not know the practical limitations of +machines with smaller memories. Any feedback would be appreciated. +

+5. Type AUTOEXEC.BAT to initialise the changes, or better still, reset +the computer. +

+6. You should now be able to run all three programs in the package from +anywhere on your disk. msf2blc, clus2blc and alscript. If you get +memory allocation errors when you try to run alscript, then use the +MAX_NSEQ and MAX_SEQ_LEN commands to reduce the default limits. If the +program still won't run, then think about buying some more memory!! +

+The programs msf2blc and clus2blc should run OK, but if you try to +process alignments that are too large for your computer, you may get a +"malloc error" which will stop the program. If this happens and you are +not using the virtual memory option discussed above, then try adding the +line set DOS4GVM=@filename to your autoexec.bat file. If you +don't have enough disk space to do this, then buy a bigger disk, or more +memory. +

+


+ Next: TECHNICAL NOTES +Up: Appendices + Previous: PostScript Fonts +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Unix Installation +Up: Appendices + Previous: 386 DOS installation +

+

TECHNICAL NOTES

+

+The executables included in this package were compiled with the WATCOM C +compiler. This is a full 32 bit compiler that makes good use of the 386 +processor and does not work on the 16 bit 286. It also has the +advantage of allowing the flat memory model to be used. In practice +this means that porting programs like alscript from Unix computers like +the Sun, is straightforward. In order to access the memory of the +computer in this way, an extra program called a dos extender is required +- this is called DOS4GW.EXE. DOS4GW is automatically invoked every time +you run one of the programs and is responsible for managing the memory and +creating the swap file discussed above. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: VAX/VMS Installation +Up: Appendices + Previous: TECHNICAL NOTES +

+

Unix Installation

+

+ +

+ALSCRIPT is distributed with executables for Sun (SunOS 4.1.3), Silicon +Graphics (IRIX 5.3), DEC ALPHA OSF/1 and Sun Solaris (2.4). The executables +are stored in the subdirectories bin/sun, bin/sgi, bin/osf and bin/sol. If +these are OK for your system, then just add the apporpriate directory to your +path, or put links to /usr/local/bin or somewhere that is on all users paths. +

+The source code for ALSCRIPT is contained in a directory hierarchy. +The top directory contains a README file and the BUILD script. +Subdirectories are: examples which contains example command and +alignment files, doc which contains and PostScript copies +of the manual - a subdirectory of this contains an HTML version of the +manual, and src which contains the source code and Makefiles for +the package. There may also be a directory called bin. If +present this will contain subdirectories with executables for the +programs in the package. Makefiles to build alscript, msf2blc, +clus2blc and alsnum are included in the src directory. Versions +for Sun (acc compiler .sun), Silicon Graphics (.sgi), DEC OSF/1 (.osf) +are included. +

+There is a utility csh script called BUILD. Simply type ./BUILD sun to +compile alscript on the Sun, ./BUILD sgi for Silicon Graphics or BUILD +gcc for use with gcc compiler. See instructions in the file BUILD. +The BUILD script will create a /bin directory and subdirecotry +if not already present. You can create makefiles for different computers +and the BUILD script should still function. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Alternative ways of +Up: Appendices + Previous: Unix Installation +

+

VAX/VMS Installation

+

+ +

+The standard VAX C compiler is not ANSI. Accordingly, ALSCRIPT will require +changes to the source code to compile on a VAX. +

+The DEC C++ compiler works OK for alscript. Alscript will also compile +on Dec ALPHA under OpenVMS. A descrip.mms file is included for this +purpose. +

+WARNING: I've not tested Version 2.0 of ALSCRIPT on VMS +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Program Crashes and +Up: Appendices + Previous: VAX/VMS Installation +

+

Alternative ways of invoking ALSCRIPT

+

+ +

+The documentation above describes the interactive mode of running ALSCRIPT. +However, it may be more convenient to run the program as a pipe under +Unix or MS-DOS. Examples are shown here. +

+ALSCRIPT is a program for producing pretty versions of multiple +sequence aligments. ALSCRIPT will also format single sequences. A +full description of the program is given in the file "alscript.doc". +

+Ways of running alscript: +

+

  1. + Interactive mode: just type alscript. + You will be prompted for a command file name. The command file will + define the AMPS blocfile, and name of the file to store the PostScript + output - see alscript.doc for details. +

    +

  2. + alscript <command_file> has same effect as 1, But does not prompt for + the command file + e.g. alscript example1.als +

    +

  3. + alscript -q < <blocfile> > <PostScript> + Quick mode - uses default commands, reads alignment from stdin, + writes PostScript to stdout. This mode creates a command file + called ALPSQ.COM. +

    + e.g. alscript -q < example1.blc > example1.ps +

    +

  4. + alscript -f <command_file> + Similar effect to 2. +

    +

  5. + alscript -f <command_file> -s + Silent operation: No messages are written to stderr, unless fatal. + Silent operation may be toggled by the silent_mode command + in the command file. +

    +

  6. + alscript -f <command_file> -p < <blocfile> > <PostScript> + Make alscript work like a pipe - blocfile is read from stdin, + postscript is written + to stdout. Messages are written to stderr. To supress messages include + the -s flag too +

    + e.g. alscript -f example1.als -p -s < example1.blc > example1.ps +

    +

+

+Using alscript as a pipe has the advantage of allowing the blocfile to +be created on the fly by the programs msf2blc or clus2blc. For example +if we have a GCG .msf file called "pileup.msf" we can run alscript with +default shading/fonts and send the results straight to the PostScript +printer "lpr" as follows: +

+msf2blc -q <pileup.msf | alscript -q -s | lpr +

+


+ Next: Program Crashes and +Up: Appendices + Previous: VAX/VMS Installation +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: Wish List for +Up: Appendices + Previous: Alternative ways of +

+

Program Crashes and Known Bugs

+

+ +

+We've used ALSCRIPT on Sun Workstations and Silicon Graphics for some +time, with very large alignments and command files with thousands of +commands. All seems to work OK, the program has not crashed on us at +all!! +

+However, the command interpreter in ALSCRIPT is very simple and +the program will crash if you give any command the wrong number of +arguments (e.g. leaving out the shade value in a shade_chars command). +

+If you do make the program crash, have checked all the documentation +and your numbers, and the program still crashes. Then send me +the command file and block file that causes the crash and I will try +to investigate. +

+Suggestions for improvements to the program are always welcome. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: REQUIRED STEP 1 +Up: ALSCRIPT Command Summary + Previous: ALSCRIPT Command Summary +

+

STEP 1 COMMANDS

+

+These all refer to either system settings - e.g. the maximum allowed +sequence length, or to general page layout features. e.g. the longest +and shortest side of the paper on which you are plotting. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: OPTIONAL STEP 1 +Up: ALSCRIPT Command Summary + Previous: STEP 1 COMMANDS +

+

REQUIRED STEP 1 COMMANDS

+

+BLOCK_FILE <string> +

+Gives the name of the file that contains the multiple sequence alignment +to be formatted. File names should be fully qualified i.e. not +relative to the current directory. If no block file command is given, +ALSCRIPT will expect to read the block file from standard input. +

+OUTPUT_FILE <string> +

+Defines the output file name. This command should be near the beginning of +the command list. +e.g. OUTPUT_FILE Figure1.ps +

+You MUST define an output file unless the -p option +(See Alternative ways of invoking ALSCRIPT) +is used. +

+DEFINE_FONT <int> <string> (<int>/DEFAULT)/(REL <float>) +

+Defines a font to use later: +e.g. +


+DEFINE_FONT 0 Helvetica 10
+DEFINE_FONT 2 Times-Roman 2
+

+defines font number 0 to be 10 point Helvetica, and font number 2 to be +2 point Times-Roman. Font 0 is always used as the default font. You MUST +define at least the font 0 font. +

+DEFINE_FONT 3 Times-Roman DEFAULT +

+sets font 3 to be Times-Roman at whatever the default pointsize +is as set by the POINTSIZE command. +

+DEFINE_FONT 4 Helvetica REL 0.5 +

+sets font 4 to be helvetica at half the default pointsize. +

+NOTE: Font names must be written exactly as shown in +PostScript Fonts. +

+SETUP +

+Signals the end of the STEP 1 commands. +

+

+ + +

+gjb@bioch.ox.ac.uk +
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+


+ Next: STEP 2 COMMANDS +Up: ALSCRIPT Command Summary + Previous: REQUIRED STEP 1 +

+

OPTIONAL STEP 1 COMMANDS

+

+ADD_SEQ <int> <int> +

+Allows extra sequence positions to be created in an existing alignment. This +permits additional annotations to be interspaced either above, below, or +anywhere in the middle of an alignment. For example: +

+ADD_SEQ 0 10 +

+would create an additional 10 sequences - all set to the blank character +before the first sequence in the block file that has been read in. +

+ADD_SEQ 3 1 +

+would add an extra sequence after sequence 3. +

+IMPORTANT: If you use the ADD_SEQ facility to add sequences anywhere except +after the last sequence, then remember that the sequence +numbers will alter. All formatting commands that follow this command +must use the new sequence numbering. Thus in the first example: +

+ADD_SEQ 0 10 +

+what was sequence number 1 becomes sequence 11. Sequences 1-10 are +the new blank sequences to be used for annotation. Note that the +sequence numbers only change for commands AFTER the SETUP command, +thus, multiple add_seq commands refer to the sequence number as +implied by the block file. +

+POINTSIZE <int> +

+Defines the pointsize to be used to scale the plot and space the characters. +Default is 10 point. +

+NUMBER_SEQSIf present, then the sequence number is output with the identifier +code. This is useful for finding the coordinates of residues to box +or otherwise highlight. +

+LANDSCAPE +

+Specifies that alignments will be plotted with the longest paper axis +horizontal. (Can get longer alignments on a page this way). +

+PORTRAIT +

+Specifies that alignments will be plotted with the longest paper axis vertical +(can get more sequences on a page this way). +

+IDENT_WIDTH <int> +

+Units are characters. +

+Reserves <int> characters at left of every page for plotting +identifiers. Note that not all this space need be used, if a smaller +pointsize is used to plot out the identifier codes, than is used for +the main alignment. +

+LINE_WIDTH_FACTOR <float> +

+Value greater than 0 that scales the default line width. The linewidth +is obtained by multiplying the pointsize by this factor. +

+X_SPACE_FACTOR <float> +

+Y_SPACE_FACTOR <float> +

+This determines the spacing between adjacent residues in the X and Y +directions. The spacing is calculated as: POINTSIZE + POINTSIZE * +X_SPACE_FACTORor POINTSIZE + POINTSIZE * Y_SPACE_FACTOR as +appropriate. Defaults are 0.2 and 0.0 respectively. +

+X_SHIFT_FACTOR <float> +

+Y_SHIFT_FACTOR <float> +

+These determine the shift relative to the residue drawing position +that is given to the boxing lines. The shift is calculated as +follows +

+(POINTSIZE + POINTSIZE * X_SPACE_FACTOR) * X_SHIFT_FACTOR +similarly for Y_SHIFT_FACTOR. +

+The defaults are 0.3 and 0.0 respectively. +

+Fiddling with the X_SPACE/SHIFT values is useful to fine tune the +appearance of the alignment. +

+MAX_INPUT_LEN <int> +

+Units are characters. +Defines the maximum number of characters possible in the input line +length. This must be greater than the maximum number of sequences +(MAX_NSEQ). +

+e.g. MAX_INPUT_LEN 600 +

+Increases the default value of 500 characters to 600 characters. +

+MAX_NSEQ <int> +

+Units are characters. Defines the maximum number of sequences that +may be read by the program. This parameter has a large default (500). +You may need to reduce it on computers with small memories. +

+MAX_ILEN <int> +

+Units are characters. +The maximum length allowed for a sequence identifier code. +

+MAX_SEQ_LEN <int> +

+Defines the maximum length allowed for a sequence alignment - this may +need to be reduced from the 8000 default value on smaller computers. +

+X_OFFSET <int> +Units of points (1/72 inch). +

+Defines the offset along the X-axis that the alignments will be shifted prior +to printing. Fiddle with this value to get a nice offset from the bottom left +hand corner of the page if your page size is not A4. +

+Y_OFFSET <int> +Units of points (1/72 inch). +

+As for X_OFFSET, only Y axis. +

+MAX_SIDE <int> +Units of inches. +

+Defines the length of the longest side of the printer page. +

+MIN_SIDE <int> +Units of inches. +

+Defines the length of the shortest side of the printer page. +

+VERTICAL_SPACING <int> +

+Defines the vertical spacing in character units between blocks of sequences +when more than one block will fit on a page - default is 0. +

+DEFINE_COLOUR <int> <float> <float> <float> +

+DEFINE_COLORDefines a colour - the first number is a number by which the colour will +be referred. The following three numbers are the intensities of red, +green and blue respectively. Thus: +

+DEFINE_COLOUR 1 0 0.2 0.8 +

+sets colour number 1 to be a colour with no red, 0.2 green and 0.8 blue. +The exact appearance of this colour will depend on the output device. +If you find suitable combinations of colours for your printer, then +please let me know and I shall distribute your suggestions with the +program. +

+DO_TICKSIf present, then tick marks are drawn below the numbers at the top of the page. +Otherwise no ticks are shown. +

+NUMBER_INT <int> +

+Specifies the interval for writing residue position numbers. Default is 10 +

+NO_NUMBERSSwitches all residue numbering off. +

+


+ Next: STEP 2 COMMANDS +Up: ALSCRIPT Command Summary + Previous: REQUIRED STEP 1 +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/subsubsection3_16_1_4.html b/sources/alscript/doc/alscript/subsubsection3_16_1_4.html new file mode 100644 index 0000000..25f44c4 --- /dev/null +++ b/sources/alscript/doc/alscript/subsubsection3_16_1_4.html @@ -0,0 +1,299 @@ + + STEP 2 COMMANDS + +

+


+ Next: AMPS Block file +Up: ALSCRIPT Command Summary + Previous: OPTIONAL STEP 1 +

+

STEP 2 COMMANDS

+

+All these are optional formatting commands. +

+IMPORTANT PLEASE READ THIS NOTE: +

+For those commands that accept region definitions (e.g. SURROUND_CHARS) it +is easiest to think of the region being defined in terms of X and Y +coordinates, where X is the sequence residue coordinate and Y is the +sequence number coordinate. Thus 3 7 means the 3rd residue in sequence 7. +3 7 12 42 means the rectangular box bounded by residue 3 of sequence 7 and +residue 12 of sequence 42. +

+SURROUND_CHARS <string> ALL +

+Draw lines round, but not between the characters that are in the string. +e.g. +

+SURROUND_CHARS GP ALL +

+will draw lines round all G and P characters in the alignment, but not +between adjacent G and P characters. +

+SURROUND_CHARS <string> <int> <int> <int> <int> +

+Similar command, but the surrounding is restricted to the region defined by +the four integers. +

+e.g. +

+SURROUND_CHARS ILVW 3 12 7 32 +

+would surround ILVW characters that occur in the region defined +from residue positions 3-7 of sequences 12 to 32. +

+SHADE_CHARS <string> ALL <float> +

+Shade all characters in the <string> by the grey value given by <float>. +e.g. +

+SHADE_CHARS GP ALL 0.5 +

+would shade all G and P characters in the alignment by the grey value 0.5. +

+SHADE_CHARS <string> <int> <int> <int> <int> <float> +

+restricts the shading to the region defined by the four integers. Thus +

+SHADE_CHARS ILVW 3 12 7 32 0.7 +

+would shade I L V and W characters from residues 3-7 of sequences 12-32 +inclusive with a grey value of 0.7. +

+FONT_CHARS <string> ALL <int> +

+e.g. +

+FONT_CHARS GP ALL 7 +

+would use font 7 to write out all G and P characters. Font 7 MUST have been +defined using the DEFINE_FONTcommands above. +

+FONT_CHARS <string> <int> <int> <int> <int> <int> +

+Similar to previous command, but restricts the effect to the region defined +by the first four integers. The font must have been defined by the +DEFINE_FONT command. +

+e.g. +

+FONT_CHARS ILVW 3 45 9 70 7 +

+Would set the font to 7 for I L V and W characters for residues 3-9 of +sequences 45-70 inclusive. The font must have been defined by the +DEFINE_FONT command. +

+FONT_REGION <int> <int> <int> <int> <int> +

+Define the font to use throughout the region specified by the first four +integers. +

+e.g. +

+FONT_REGION 3 12 20 40 10 +

+Use font 10 for residues from residues 3-20 of sequences 12-40. The font +must have been defined using the DEFINE_FONTcommand. +

+FONT_RESIDUE <int> <int> <int> +

+Set the font for use with a single residue position - most useful when used +with the TEXT command. +

+e.g. +

+FONT_RESIDUE 3 7 2 +

+Use font 2 for residue 3 of sequence 7. Font 2 must have been defined using +the DEFINE_FONT command. +

+LINE <string> <int> <int> <int> +

+There are four commands of this type for drawing horizontal or vertical lines +on the alignment. +

+LINE LEFT <int> <int> <int> +

+Draw a line to the left of the character positions indicated. +

+e.g. +

+LINE LEFT 3 12 24 +

+Draw a vertical line starting at residue 3 of sequence 12 and ending at +residue 3 of sequence 24. +

+LINE TOP 3 12 24 +

+Draw a horizontal line above the character positions from residue 3 of +sequence 12 to residue 24 of sequence 12. +

+Similar commands are: +

+LINE BOTTOM <int> <int> <int> Draw a line at bottom of character position. +

+LINE RIGHT <int> <int> <int> Draw a line at right of character position. +

+BOX_REGION <int> <int> <int> <int> +

+Draw a box around the region indicated by the four integers. +

+e.g. +

+BOX_REGION 2 5 30 7 +

+Would box from residue 2 of sequence 5 to residue 30 of sequence 7. +

+SHADE_REGION <int> <int> <int> <int> <float> +

+Shade the region indicated by the integers with the grey value shown by the +float. +e.g. +

+SHADE_REGION 30 40 35 46 0.2 +

+Would shade from residue 30-35 of sequences 40-46 with a grey value of 0.2. +

+SHADE_RES <int> <int> <float> +

+Shade just one amino acid with the grey value. +

+e.g. +

+SHADE_RES 3 4 0.7 +

+Shades residue 3 of sequence 7. (Note: this can also be achieved with the +

+SHADE_REGION command, but requires 2 extra numbers) +

+TEXT <int> <int> <qstring> +

+Place the text string at the location indicated. +

+e.g. +

+TEXT 30 70 "Active Site His" +

+would put the text Active Site His starting at position 30 of sequence +70. (Use FONT_RESIDUE or FONT_REGION commands to set the font of the +text). Text added with the TEXT command will not be split across page +breaks, so you may in some circumstances need to fiddle a little with +the location/pointsize for the text to get the desired result. +

+ID_FONT ALL <int> +

+Set the font for all identifier codes to the font number shown by <int>. +e.g. +

+ID_FONT ALL 3 +

+Would set all the identifier codes to font 3. +

+ID_FONT <int> <int> +

+Set the font for a specific identifier to font number. +e.g. +

+ID_FONT 12 4 +

+Use font 4 for the identifer of sequence 12, default font for all other +identifiers. +

+SUB_CHARS ALL <char> <char> +

+Substitute the characters indicated. +

+e.g. +

+SUB_CHARS ALL + * +

+would change all occurences of + to * in the alignment. +

+SUB_CHARS <int> <int> <int> <int> <char> <char> +

+restrict the substitution to the region shown. +

+e.g. +

+SUB_CHARS 1 1 7 8 %* +

+would substitute * for %from residue 1-7 of sequences 1-8. +NOTE: To substitute for or with the space character use the word SPACE. +e.g. to change all space characters to -. +

+SUB_CHARS ALL SPACE - +

+SUB_ID <int> <qstring> +

+Replace the numbered identifier by the string. +e.g. +

+SUB_ID 34 "Predicted Secondary Structure" +

+would replace whatever the identifier of sequence 34 was, by the text shown. +This is useful when used in conjunction with the ADD_SEQ command shown under +the STEP 1 commands. +

+INVERSE_CHARS <string> ALL/Range (similar syntax to FONT_CHARS but no +font number) +

+Print the selected characters in white. This clearly will only work +if you first use the SHADE_CHARScommand to shade the characters with +something other than white. +

+CCOL_CHARS <string> ALL <int> +

+Colour all characters in the <string> by the colour defined by <int>. +

+e.g. +

+CCOL_CHARS GP ALL 12 +

+would colour all G and P characters in the alignment by the colour 12. +This colour MUST have been defined by the DEFINE_COLOUR command. +

+CCOL_CHARS <string> <int> <int> <int> <int> <int> +

+restricts the colouring to the region defined by the four integers. Thus +

+CCOL_CHARS ILVW 3 12 7 32 7 +

+would colour I L V and W characters from residues 3-7 of sequences 12-32 +inclusive with the colour 7. +

+SCOL_CHARS: This has identical syntax to SCOL_CHARS, but colours the +background of the character, rather than the letter itself. +

+COLOUR_REGION <int> <int> <int> <int> <int> +

+COLOR_REGIONColour the region indicated by the integers with the colour number given +as the last number. +

+e.g. +

+COLOUR_REGION 30 40 35 46 2 +

+Would colour from residue 30-35 of sequences 40-46 with the colour 2. +

+COLOUR_RES <int> <int> <int> +

+Colour just one amino acid with the defined colour. +

+e.g. +

+COLOUR_RES 3 4 7 +

+Colours residue 3 of sequence 7. (Note: this can also be achieved with the +COLOUR_REGION command, but requires 2 extra numbers) +

+


+ Next: AMPS Block file +Up: ALSCRIPT Command Summary + Previous: OPTIONAL STEP 1 +

+

+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/doc/alscript/tableofcontents3_1.html b/sources/alscript/doc/alscript/tableofcontents3_1.html new file mode 100644 index 0000000..7d5976d --- /dev/null +++ b/sources/alscript/doc/alscript/tableofcontents3_1.html @@ -0,0 +1,67 @@ + +Contents + +

+


+ Next: Update History +Up: No Title + Previous: No Title +

+

Contents

+ +
+ + +

+gjb@bioch.ox.ac.uk +
\ No newline at end of file diff --git a/sources/alscript/examples/demo/example.als b/sources/alscript/examples/demo/example.als new file mode 100644 index 0000000..0416736 --- /dev/null +++ b/sources/alscript/examples/demo/example.als @@ -0,0 +1,11 @@ +#Comments in ALscript command files start with a # +# +#Commands are free format - separated by blank, tab or comma characters +# +BLOCK_FILE example.blc #define the block file to format +OUTPUT_FILE example.ps #where to put the result +LANDSCAPE #landscape paper orientation +POINTSIZE 24 #12 point default pointsize +DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica +SETUP #Tell the program to get on with the formatting. + diff --git a/sources/alscript/examples/demo/example.blc b/sources/alscript/examples/demo/example.blc new file mode 100644 index 0000000..30cfdd3 --- /dev/null +++ b/sources/alscript/examples/demo/example.blc @@ -0,0 +1,33 @@ +A simple example block file. The important parts are the lines that +start with the greater than character - these are identifer lines for the +sequences. The first star is above the position of the first sequence +in the alingmnet which is written VERTICALLY down the page. The second +star shows the end of the alignment. + +Anything else can be written here, except greater than signs and stars. + +This is the format produced by the AMPS package for multiple +sequence alignment. + + +>one +>two +>three +>four +>five +* +ABCDE +EDFTD +AAAAA +LLL +PPPPP +GGGGG +SSSSS +KLMNT +TTLPQ +SLNST +TTLSN +LQPPL +LLLLL +TTTTT +* diff --git a/sources/alscript/examples/demo/example.ps b/sources/alscript/examples/demo/example.ps new file mode 100644 index 0000000..c17096b --- /dev/null +++ b/sources/alscript/examples/demo/example.ps @@ -0,0 +1,103 @@ +%!PS-Adobe-2.0 +%%Creator: ALSCRIPT Version 2.04 - 28 June 1996 +%%Title: Alscript Output +% Barton, G. J. (1993) +% ALSCRIPT - A Tool to format multiple sequence alignments +% Protein Engineering, 6, 37-40 +% +/P {moveto show} def +/L {moveto lineto stroke} def +/ML {moveto lineto lineto lineto lineto} def +2 setlinecap +/C99 {1.000000 1.000000 1.000000 setrgbcolor } def +/C100 {0.000000 0.000000 0.000000 setrgbcolor } def +120 currentscreen 3 -1 roll pop setscreen +/F0 {/Helvetica findfont 24.00 scalefont setfont } def +%%Page: ? 1 +508 30 translate +90 rotate +F0 + 0 288 translate +2 setlinecap + 1.20 setlinewidth + 0 0 moveto (five +) show + 0 24 moveto (four +) show + 0 48 moveto (three +) show + 0 72 moveto (two +) show + 0 96 moveto (one +) show +(E) 280 0 P +(D) 308 0 P +(A) 336 0 P +(P) 392 0 P +(G) 420 0 P +(S) 448 0 P +(T) 476 0 P +(Q) 504 0 P +(T) 532 0 P +(N) 560 0 P +(L) 588 0 P +(L) 616 0 P +(T) 644 0 P +(D) 280 24 P +(T) 308 24 P +(A) 336 24 P +(P) 392 24 P +(G) 420 24 P +(S) 448 24 P +(N) 476 24 P +(P) 504 24 P +(S) 532 24 P +(S) 560 24 P +(P) 588 24 P +(L) 616 24 P +(T) 644 24 P +(C) 280 48 P +(F) 308 48 P +(A) 336 48 P +(L) 364 48 P +(P) 392 48 P +(G) 420 48 P +(S) 448 48 P +(M) 476 48 P +(L) 504 48 P +(N) 532 48 P +(L) 560 48 P +(P) 588 48 P +(L) 616 48 P +(T) 644 48 P +(B) 280 72 P +(D) 308 72 P +(A) 336 72 P +(L) 364 72 P +(P) 392 72 P +(G) 420 72 P +(S) 448 72 P +(L) 476 72 P +(T) 504 72 P +(L) 532 72 P +(T) 560 72 P +(Q) 588 72 P +(L) 616 72 P +(T) 644 72 P +(A) 280 96 P +(E) 308 96 P +(A) 336 96 P +(L) 364 96 P +(P) 392 96 P +(G) 420 96 P +(S) 448 96 P +(K) 476 96 P +(T) 504 96 P +(S) 532 96 P +(T) 560 96 P +(L) 588 96 P +(L) 616 96 P +(T) 644 96 P + 280 120 moveto (1) show + 532 120 moveto (10) show +showpage diff --git a/sources/alscript/examples/demo/example1.als b/sources/alscript/examples/demo/example1.als new file mode 100644 index 0000000..96302b7 --- /dev/null +++ b/sources/alscript/examples/demo/example1.als @@ -0,0 +1,184 @@ +#SILENT_MODE +# Illustration of ALSCRIPT Command File +# +# As shown in: Barton, G. J. (1992) +# ALSCRIPT- A tool to format multiple sequence alignments +# Protein Engineering (in press) +# +# example 1 # Comment lines begin with the # symbol +# +# +# (A) Input and output files: +# +BLOCK_FILE example1.blc # Define the block file to format +OUTPUT_FILE example1.ps # Where to put the result +# +MAX_SIDE 11 # Fiddle with margins... +Y_OFFSET 50 # This is just to fill the page with the +X_OFFSET 80 # Alignment. Defaults normally work OK +# +# (B) Page Layout and overall spacing: +# +ADD_SEQ 38 1 # Make space after sequence 38 +ADD_SEQ 39 3 # Make space after sequence 39 +ADD_SEQ 69 1 +PORTRAIT # Portrait paper orientation +POINTSIZE 10 # 10 point default pointsize +#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot +IDENT_WIDTH 8 +# +# (C) Font definitions: +# +DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize +DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT +DEFINE_FONT 4 Times-Bold DEFAULT +DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT +DEFINE_FONT 6 Times-Roman DEFAULT +SETUP # Tell the program to get on with the formatting +# +# (D) Some formatting commands: +# +SURROUND_CHARS LIV ALL # Surround all L and Y characters +FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters +FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps +FONT_CHARS KR ALL 4 # Show K and R in Times-Bold +BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 +SHADE_CHARS + ALL 0.0 # Shade all + characters black +SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 +LINE TOP 2 40 8 # Draw horizontal lines to +LINE BOTTOM 2 40 8 # Join up surrounded numbers +TEXT 2 42 "Helix Pattern" +TEXT 12 42 "Glycine Loop" # Add text annotations +TEXT 21 42 "Helix Pattern" +SUB_ID 49 "Helix Predictions" +SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms +SUB_ID 68 "Turn Predictions" +INVERSE_CHARS DE ALL +SHADE_CHARS D ALL 0.5 +SHADE_CHARS E ALL 0.0 +# +# (E) Now make the prediction histograms nicer. +# +SURROUND_CHARS H 1 44 27 53 +SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey +SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" +# +SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions +SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions +SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions +# +SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions +SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions +SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions +# +SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction +SHADE_CHARS HY 1 75 27 75 0.5 +SHADE_CHARS T 1 75 27 75 0.9 +# +# +# Now change the identifier codes +# +ID_FONT ALL 6 +SUB_ID 1 "Annexin I H4" +SUB_ID 2 "Annexin I M4" +SUB_ID 3 "Annexin I R4" +SUB_ID 4 "Annexin V R4" +SUB_ID 5 "Annexin V H4" +SUB_ID 6 "Annexin V C4" +SUB_ID 7 "Annexin II H4" +SUB_ID 8 "Annexin II B4" +SUB_ID 9 "Annexin II M4" +SUB_ID 10 "Annexin IV P4" +SUB_ID 11 "Annexin IV B4" +SUB_ID 12 "Annexin IV H4" +SUB_ID 13 "Annexin VI H4" +SUB_ID 14 "Annexin VI M4" +SUB_ID 15 "Annexin VI H8" +SUB_ID 16 "Annexin VI M8" +SUB_ID 17 "Annexin V BH4" +SUB_ID 18 "Annexin VII 4" +SUB_ID 19 "Annexin III H4" +SUB_ID 20 "Annexin III R4" +SUB_ID 21 "Annexin II M3" +SUB_ID 22 "Annexin II H3" +SUB_ID 23 "Annexin II B3" +SUB_ID 24 "Annexin I H3" +SUB_ID 25 "Annexin I M3" +SUB_ID 26 "Annexin I R3" +SUB_ID 27 "Annexin V C3" +SUB_ID 28 "Annexin V H3" +SUB_ID 29 "Annexin V R3" +SUB_ID 30 "Annexin VI H3" +SUB_ID 31 "Annexin VI M3" +SUB_ID 32 "Annexin IV H3" +SUB_ID 33 "Annexin IV B3" +SUB_ID 34 "Annexin IV P3" +SUB_ID 35 "Annexin V BH3" +SUB_ID 36 "Annexin III H3" +SUB_ID 37 "Annexin III R3" +SUB_ID 38 "Annexin VII 3" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/examples/demo/example1.blc b/sources/alscript/examples/demo/example1.blc new file mode 100644 index 0000000..517ef70 --- /dev/null +++ b/sources/alscript/examples/demo/example1.blc @@ -0,0 +1,102 @@ +>L1H4 +>L1M4 +>L1R4 +>L5R4 +>L5H4 +>L5C4 +>L2H4 +>L2B4 +>L2M4 +>L4P4 +>L4B4 +>L4H4 +>L6H4 +>L6M4 +>L6H8 +>L6M8 +>L5BH4 +>p474 +>L3H4 +>L3R4 +>L2M3 +>L2H3 +>L2B3 +>L1H3 +>L1M3 +>L1R3 +>L5C3 +>L5H3 +>L5R3 +>L6H3 +>L6M3 +>L4H3 +>L4B3 +>L4P3 +>L5BH3 +>L3H3 +>L3R3 +>p473 +>Conservation +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> + * + FFFLLFFFFFFFFFFFFFLLDDDDDDDDDDDDDDDDDD HHHHHHH E TTTTH + AAAAAAAAAAAAAAAAAAAAAAAAAAAAAVVAAAAAAA7 HHHHHHHH TTTH + EEEEEEDDDEEEEEDDEEEGRRRRRRQQQQQQQQQQQQ HHHHHHHH TTH + KKKTTTRRRRRKRRKKRRRREDDAAAVAADDDDDDITR HHHHHHHHH E TTTH + LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLL+ HHHHHHHHH E TTTH + HYYYYYYYYYYYFFYYYYHHYYYYYYFFFYYYYYYYYY HHHHHHHHH E TTH + QEEYYYDDDKKKKKKKYYRQDDDEEERQQEEEEEAKDQ HHHHHHHHH E TTH + AAAAASSSSSSSAASSAAAAAAAAAAAAAAAAAAAAAA7 HHHHHHHH E TTH + GGGGGGGGGGGGGGGGGG H T + MMMMMMMMMMMMMMMMMMLLVVVEEEEEEEEEEEEEEE HHHHHHHH E TTTH + KKKKKKKKKKKKKKKKKKKKKKKRRRLLLLLKKKKNKG HHHHHHH E TTTTH + GGGGGGGGGGGGGGGGGGGGRRRRRRKKKKKKKKIRKR HHH TTTTT + VAAAAAKKKLLLLLAAAAIAKKKKKKWWWWWWWWRWWL HHH TTTTT + GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG+ HH TTTTTTT + TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5 HHHH TTTTTTTTTT + RRRDDDRRRDDDRRDDRDDDDDDDDDDDDDDDDDDDDD HHHHH TTTTTTTTTT + HHHDDDDDDDDDDDEEDDEEVVVVVVEEEEEEEEEEEE HHHHHHHH E TTTTTTTTTT + KKKHHDKKKNDNNNKKGSFFPPPNNNEEEAAVVVMDDS HHHHHHH E TTTTTTTY + AATTTTVVVTTTTTTTTTTTKKKVVVTKKQQKKKKKKC HHHHHHHH EEE TTTTTH + LLLLLLLLLLLLLLLLLLLLWWWFFFFFFFFFFFFFFF6 HHHHHHHH EEEE TTH + IIIIIIIIIIIIIITTIVNNIIINTNIIIIILLLITTN5 HHHHHHH EEEE TH + RRRRRRRRRRRRRRRRRRRRSSSTTTTTTYYTTTTEEM HHHHHHH EEEEE H + IIIVVVIIIVVVIIIVNIIIIIIIIIIIIIIVVVIIII5 HHHHHH EEEEE H + MMMIMMMMMMMMMMMMIVMMMMMLLLLFLLLLLLLLLL6 HHHHHH EEEEE TH + VVVVVVVVVVVVVVVVVVVVTTTTTTGGGGGCCCCCCA6 HHHHH EEEE TTTTH + SSSSSSSSSSSSSSSSSTSSEEETSTTTTNNSSSTLLT HHHH EE TTTTTT + RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR+ HHHH EE TTTTTTTTTT + * + + + diff --git a/sources/alscript/examples/demo/example1.ps b/sources/alscript/examples/demo/example1.ps new file mode 100644 index 0000000..fdf3045 --- /dev/null +++ b/sources/alscript/examples/demo/example1.ps @@ -0,0 +1,5460 @@ +%!PS-Adobe +%%Creator: ALSCRIPT Version 2.0 +%%Title: Alscript Output +% Barton, G. 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238.80 L + C100 + 94.56 238.80 94.56 228.80 L + C100 + 94.56 228.80 94.56 218.80 L + C100 + 94.56 218.80 106.56 218.80 L + C100 + 94.56 128.80 94.56 118.80 L + C100 + 106.56 128.80 106.56 118.80 L + C100 + 94.56 128.80 106.56 128.80 L + C100 + 94.56 118.80 106.56 118.80 L + C100 + 94.56 58.80 94.56 48.80 L + C100 + 106.56 58.80 106.56 48.80 L + C100 + 94.56 58.80 106.56 58.80 L + C100 + 94.56 48.80 94.56 38.80 L + C100 + 94.56 38.80 94.56 28.80 L + C100 + 94.56 28.80 94.56 18.80 L + C100 + 94.56 18.80 106.56 18.80 L + F6 + 0 0 moveto (Summary Prediction) show +F0 + F6 + 0 10 moveto ( ) show +F0 + F6 + 0 20 moveto ( ) show +F0 + F6 + 0 30 moveto ( ) show +F0 + F6 + 0 40 moveto ( ) show +F0 + F6 + 0 50 moveto ( ) show +F0 + F6 + 0 60 moveto ( ) show +F0 + F6 + 0 70 moveto (Turn Predictions) show +F0 + F6 + 0 80 moveto ( ) show +F0 + F6 + 0 90 moveto ( ) show +F0 + F6 + 0 100 moveto ( ) show +F0 + F6 + 0 110 moveto ( ) show +F0 + F6 + 0 120 moveto ( ) show +F0 + F6 + 0 130 moveto ( ) show +F0 + F6 + 0 140 moveto ( ) show +F0 + F6 + 0 150 moveto ( ) show +F0 + F6 + 0 160 moveto ( ) show +F0 + F6 + 0 170 moveto (Sheet Predictions) show +F0 + F6 + 0 180 moveto ( ) show +F0 + F6 + 0 190 moveto ( ) show +F0 + F6 + 0 200 moveto ( ) show +F0 + F6 + 0 210 moveto ( ) show +F0 + F6 + 0 220 moveto ( ) show +F0 + F6 + 0 230 moveto ( ) show +F0 + F6 + 0 240 moveto ( ) show +F0 + F6 + 0 250 moveto ( ) show +F0 + F6 + 0 260 moveto (Helix Predictions) show +F0 + F6 + 0 270 moveto ( ) show +F0 + F6 + 0 280 moveto ( ) show +F0 + F6 + 0 290 moveto ( ) show +F0 + F6 + 0 300 moveto ( ) show +F0 + F6 + 0 310 moveto ( ) show +F0 + F6 + 0 320 moveto ( ) show +F0 + F6 + 0 330 moveto ( ) show +F0 + F6 + 0 340 moveto ( ) show +F0 + F6 + 0 350 moveto (Conservation +) show +F0 + F6 + 0 360 moveto ( ) show +F0 + F6 + 0 370 moveto (Annexin VII 3) show +F0 + F6 + 0 380 moveto (Annexin III R3) show +F0 + F6 + 0 390 moveto (Annexin III H3) show +F0 + F6 + 0 400 moveto (Annexin V BH3) show +F0 + F6 + 0 410 moveto (Annexin IV P3) show +F0 + F6 + 0 420 moveto (Annexin IV B3) show +F0 + F6 + 0 430 moveto (Annexin IV H3) show +F0 + F6 + 0 440 moveto (Annexin VI M3) show +F0 + F6 + 0 450 moveto (Annexin VI H3) show +F0 + F6 + 0 460 moveto (Annexin V R3) show +F0 + F6 + 0 470 moveto (Annexin V H3) show +F0 + F6 + 0 480 moveto (Annexin V C3) show +F0 + F6 + 0 490 moveto (Annexin I R3) show +F0 + F6 + 0 500 moveto (Annexin I M3) show +F0 + F6 + 0 510 moveto (Annexin I H3) show +F0 + F6 + 0 520 moveto (Annexin II B3) show +F0 + F6 + 0 530 moveto (Annexin II H3) show +F0 + F6 + 0 540 moveto (Annexin II M3) show +F0 + F6 + 0 550 moveto (Annexin III R4) show +F0 + F6 + 0 560 moveto (Annexin III H4) show +F0 + F6 + 0 570 moveto (Annexin VII 4) show +F0 + F6 + 0 580 moveto (Annexin V BH4) show +F0 + F6 + 0 590 moveto (Annexin VI M8) show +F0 + F6 + 0 600 moveto (Annexin VI H8) show +F0 + F6 + 0 610 moveto (Annexin VI M4) show +F0 + F6 + 0 620 moveto (Annexin VI H4) show +F0 + F6 + 0 630 moveto (Annexin IV H4) show +F0 + F6 + 0 640 moveto (Annexin IV B4) show +F0 + F6 + 0 650 moveto (Annexin IV P4) show +F0 + F6 + 0 660 moveto (Annexin II M4) show +F0 + F6 + 0 670 moveto (Annexin II B4) show +F0 + F6 + 0 680 moveto (Annexin II H4) show +F0 + F6 + 0 690 moveto (Annexin V C4) show +F0 + F6 + 0 700 moveto (Annexin V H4) show +F0 + F6 + 0 710 moveto (Annexin V R4) show +F0 + F6 + 0 720 moveto (Annexin I R4) show +F0 + F6 + 0 730 moveto (Annexin I M4) show +F0 + F6 + 0 740 moveto (Annexin I H4) show +F0 + (H) 96 0 P +(H) 108 0 P +(H) 120 0 P +(H) 132 0 P +(H) 144 0 P +(H) 156 0 P +(H) 168 0 P +(H) 180 0 P +(H) 204 0 P +(H) 216 0 P +( +) 228 0 P +(T) 264 0 P +(T) 276 0 P +(T) 288 0 P +(Y) 300 0 P +(H) 312 0 P +(H) 324 0 P +(H) 336 0 P +(H) 348 0 P +(H) 360 0 P +(H) 372 0 P +(H) 384 0 P +(T) 396 0 P +(T) 408 0 P +C100 + 108 330 moveto (Helix Pattern) show + 228 330 moveto (Glycine Loop) show + 336 330 moveto (Helix Pattern) show +(7) 108 350 P +(+) 144 350 P +(7) 180 350 P +(+) 252 350 P +(5) 264 350 P +(6) 324 350 P +(5) 336 350 P +(5) 360 350 P +(6) 372 350 P +(6) 384 350 P +(+) 408 350 P + 1.00 setgray +(D) 96 370 P + 0.00 setgray +F1 + (A) 108 370 P +F0 + (Q) 120 370 P +F4 + (R) 132 370 P +F0 + (L) 144 370 P +(Y) 156 370 P +(Q) 168 370 P +F1 + (A) 180 370 P +F0 + (G) 192 370 P + 1.00 setgray +(E) 204 370 P + 0.00 setgray +(G) 216 370 P +F4 + (R) 228 370 P +F0 + (L) 240 370 P +(G) 252 370 P +(T) 264 370 P + 1.00 setgray +(D) 276 370 P + 0.00 setgray + 1.00 setgray +(E) 288 370 P + 0.00 setgray +F1 + (S) 300 370 P +F0 + (C) 312 370 P +(F) 324 370 P +F1 + (N) 336 370 P +F0 + (M) 348 370 P +F5 + (I) 360 370 P +F0 + (L) 372 370 P +F1 + (A) 384 370 P +F0 + (T) 396 370 P +F4 + (R) 408 370 P +F0 + 1.00 setgray +(D) 96 380 P + 0.00 setgray +F1 + (A) 108 380 P +F0 + (Q) 120 380 P +(T) 132 380 P +(L) 144 380 P +(Y) 156 380 P + 1.00 setgray +(D) 168 380 P + 0.00 setgray +F1 + (A) 180 380 P +F0 + (G) 192 380 P + 1.00 setgray +(E) 204 380 P + 0.00 setgray +F4 + (K) 216 380 P +F0 + F4 + (K) 228 380 P +F0 + (W) 240 380 P +(G) 252 380 P +(T) 264 380 P + 1.00 setgray +(D) 276 380 P + 0.00 setgray + 1.00 setgray +(E) 288 380 P + 0.00 setgray + 1.00 setgray +(D) 300 380 P + 0.00 setgray +F4 + (K) 312 380 P +F0 + (F) 324 380 P +(T) 336 380 P + 1.00 setgray +(E) 348 380 P + 0.00 setgray +F5 + (I) 360 380 P +F0 + (L) 372 380 P +(C) 384 380 P +(L) 396 380 P +F4 + (R) 408 380 P +F0 + 1.00 setgray +(D) 96 390 P + 0.00 setgray +F1 + (A) 108 390 P +F0 + (Q) 120 390 P +F5 + (I) 132 390 P +F0 + (L) 144 390 P +(Y) 156 390 P +F4 + (K) 168 390 P +F0 + F1 + (A) 180 390 P +F0 + (G) 192 390 P + 1.00 setgray +(E) 204 390 P + 0.00 setgray +F1 + (N) 216 390 P +F0 + F4 + (R) 228 390 P +F0 + (W) 240 390 P +(G) 252 390 P +(T) 264 390 P + 1.00 setgray +(D) 276 390 P + 0.00 setgray + 1.00 setgray +(E) 288 390 P + 0.00 setgray + 1.00 setgray +(D) 300 390 P + 0.00 setgray +F4 + (K) 312 390 P +F0 + (F) 324 390 P +(T) 336 390 P + 1.00 setgray +(E) 348 390 P + 0.00 setgray +F5 + (I) 360 390 P +F0 + (L) 372 390 P +(C) 384 390 P +(L) 396 390 P +F4 + (R) 408 390 P +F0 + 1.00 setgray +(D) 96 400 P + 0.00 setgray +F1 + (A) 108 400 P +F0 + (Q) 120 400 P + 1.00 setgray +(D) 132 400 P + 0.00 setgray +(L) 144 400 P +(Y) 156 400 P +F1 + (A) 168 400 P +F0 + F1 + (A) 180 400 P +F0 + (G) 192 400 P + 1.00 setgray +(E) 204 400 P + 0.00 setgray +F4 + (K) 216 400 P +F0 + F5 + (I) 228 400 P +F0 + F4 + (R) 240 400 P +F0 + (G) 252 400 P +(T) 264 400 P + 1.00 setgray +(D) 276 400 P + 0.00 setgray + 1.00 setgray +(E) 288 400 P + 0.00 setgray +(M) 300 400 P +F4 + (K) 312 400 P +F0 + (F) 324 400 P +F5 + (I) 336 400 P +F0 + (T) 348 400 P +F5 + (I) 360 400 P +F0 + (L) 372 400 P +(C) 384 400 P +(T) 396 400 P +F4 + (R) 408 400 P +F0 + 1.00 setgray +(D) 96 410 P + 0.00 setgray +F1 + (A) 108 410 P +F0 + (Q) 120 410 P + 1.00 setgray +(D) 132 410 P + 0.00 setgray +(L) 144 410 P +(Y) 156 410 P + 1.00 setgray +(E) 168 410 P + 0.00 setgray +F1 + (A) 180 410 P +F0 + (G) 192 410 P + 1.00 setgray +(E) 204 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b/sources/alscript/examples/demo/example2.als new file mode 100644 index 0000000..642a7a2 --- /dev/null +++ b/sources/alscript/examples/demo/example2.als @@ -0,0 +1,24 @@ +# FILE example2.com +# +#Commands are free format - separated by blank, tab or comma characters +# +BLOCK_FILE example.blc #define the block file to format +OUTPUT_FILE example2.ps #where to put the result +LANDSCAPE #landscape paper orientation +POINTSIZE 12 #12 point default pointsize +DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica +DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica +DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic +NUMBER_SEQS #Number the sequences at left hand side +SETUP #Tell the program to get on with the formatting. +# +#step 2 commands come after the SETUP command +# +#Here are some examples... +# +SURROUND_CHARS GP ALL #draw lines around all G and P +SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6 +BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20 +FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters +ID_FONT ALL 1 #set identifiers in font 1 diff --git a/sources/alscript/examples/demo/example3.als b/sources/alscript/examples/demo/example3.als new file mode 100644 index 0000000..bb9de1f --- /dev/null +++ b/sources/alscript/examples/demo/example3.als @@ -0,0 +1,209 @@ +# Illustration of ALSCRIPT Command File +# +# As shown in: Barton, G. J. (1992) +# ALSCRIPT- A tool to format multiple sequence alignments +# Protein Engineering (in press) +# +# Modified version - includes colour commands +# +# +# example 1 # Comment lines begin with the # symbol +# +# +# (A) Input and output files: +# +BLOCK_FILE example1.blc # Define the block file to format +OUTPUT_FILE example3.ps # Where to put the result +# +MAX_SIDE 11 # Fiddle with margins... +Y_OFFSET 50 # This is just to fill the page with the +X_OFFSET 80 # Alignment. Defaults normally work OK +# +# (B) Page Layout and overall spacing: +# +ADD_SEQ 38 1 # Make space after sequence 38 +ADD_SEQ 39 3 # Make space after sequence 39 +ADD_SEQ 69 1 +PORTRAIT # Portrait paper orientation +POINTSIZE 10 # 10 point default pointsize +#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot +IDENT_WIDTH 8 +# +# (C) Font definitions: +# +DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize +DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT +DEFINE_FONT 4 Times-Bold DEFAULT +DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT +DEFINE_FONT 6 Times-Roman DEFAULT +# +# COLOUR DEFINITIONS give a colour number, then red, green, blue intensities +# +# red +DEFINE_COLOUR 0 1 0 0 +# green +DEFINE_COLOUR 1 0 1 0 +# blue +DEFINE_COLOUR 2 0 0 1 +# yellow +DEFINE_COLOUR 3 1 1 0 +# magenta +DEFINE_COLOUR 4 1 0 1 +# cyan +DEFINE_COLOUR 5 0 1 1 +# +DEFINE_COLOUR 6 0.7 0.3 0 +#single_page +#background_colour 100 +SETUP # Tell the program to get on with the formatting +#background_region -10 -10 468 792 +# +# (D) Some formatting commands: +# +SURROUND_CHARS LIV ALL # Surround all L and Y characters +FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters +FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps +FONT_CHARS KR ALL 4 # Show K and R in Times-Bold +Ccol_CHARS LIV ALL 1 +Scol_CHARS ASN ALL 0 +BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 +SHADE_CHARS + ALL 0.0 # Shade all + characters black +COLOUR_REGION 14 1 16 38 2 +SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 +LINE TOP 2 40 8 # Draw horizontal lines to +LINE BOTTOM 2 40 8 # Join up surrounded numbers +TEXT 2 42 "Helix Pattern" +TEXT 12 42 "Glycine Loop" # Add text annotations +TEXT 21 42 "Helix Pattern" +SUB_ID 49 "Helix Predictions" +SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms +SUB_ID 68 "Turn Predictions" +INVERSE_CHARS DE ALL +SHADE_CHARS D ALL 0.5 +SHADE_CHARS E ALL 0.0 +# +# (E) Now make the prediction histograms nicer. +# +SURROUND_CHARS H 1 44 27 53 +Scol_CHARS H 1 44 27 53 3 # Shade the Helix predictions 0.5 grey +SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" +# +SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions +Scol_CHARS E 1 54 27 63 4 # Colour beta blue +SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions +# +SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions +Scol_CHARS T 1 64 27 73 6 # Shade the Turn Predictions +SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions +# +SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction +SHADE_CHARS HY 1 75 27 75 0.5 +SHADE_CHARS T 1 75 27 75 0.9 +# +# +# Now change the identifier codes +# +ID_FONT ALL 6 +SUB_ID 1 "Annexin I H4" +SUB_ID 2 "Annexin I M4" +SUB_ID 3 "Annexin I R4" +SUB_ID 4 "Annexin V R4" +SUB_ID 5 "Annexin V H4" +SUB_ID 6 "Annexin V C4" +SUB_ID 7 "Annexin II H4" +SUB_ID 8 "Annexin II B4" +SUB_ID 9 "Annexin II M4" +SUB_ID 10 "Annexin IV P4" +SUB_ID 11 "Annexin IV B4" +SUB_ID 12 "Annexin IV H4" +SUB_ID 13 "Annexin VI H4" +SUB_ID 14 "Annexin VI M4" +SUB_ID 15 "Annexin VI H8" +SUB_ID 16 "Annexin VI M8" +SUB_ID 17 "Annexin V BH4" +SUB_ID 18 "Annexin VII 4" +SUB_ID 19 "Annexin III H4" +SUB_ID 20 "Annexin III R4" +SUB_ID 21 "Annexin II M3" +SUB_ID 22 "Annexin II H3" +SUB_ID 23 "Annexin II B3" +SUB_ID 24 "Annexin I H3" +SUB_ID 25 "Annexin I M3" +SUB_ID 26 "Annexin I R3" +SUB_ID 27 "Annexin V C3" +SUB_ID 28 "Annexin V H3" +SUB_ID 29 "Annexin V R3" +SUB_ID 30 "Annexin VI H3" +SUB_ID 31 "Annexin VI M3" +SUB_ID 32 "Annexin IV H3" +SUB_ID 33 "Annexin IV B3" +SUB_ID 34 "Annexin IV P3" +SUB_ID 35 "Annexin V BH3" +SUB_ID 36 "Annexin III H3" +SUB_ID 37 "Annexin III R3" +SUB_ID 38 "Annexin VII 3" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/examples/demo/example3.ps b/sources/alscript/examples/demo/example3.ps new file mode 100644 index 0000000..8ecd991 --- /dev/null +++ b/sources/alscript/examples/demo/example3.ps @@ -0,0 +1,10686 @@ +%!PS-Adobe-2.0 +%%Creator: ALSCRIPT Version 2.04 - 28 June 1996 +%%Title: Alscript Output +% Barton, G. 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94.56 118.80 L + C100 + 106.56 128.80 106.56 118.80 L + C100 + 94.56 128.80 106.56 128.80 L + C100 + 94.56 118.80 106.56 118.80 L + C100 + 94.56 58.80 94.56 48.80 L + C100 + 106.56 58.80 106.56 48.80 L + C100 + 94.56 58.80 106.56 58.80 L + C100 + 94.56 48.80 94.56 38.80 L + C100 + 94.56 38.80 94.56 28.80 L + C100 + 94.56 28.80 94.56 18.80 L + C100 + 94.56 18.80 106.56 18.80 L + C100 + F6 + 0 0 moveto (Summary Prediction) show +F0 + C100 + F6 + 0 10 moveto ( ) show +F0 + C100 + F6 + 0 20 moveto ( ) show +F0 + C100 + F6 + 0 30 moveto ( ) show +F0 + C100 + F6 + 0 40 moveto ( ) show +F0 + C100 + F6 + 0 50 moveto ( ) show +F0 + C100 + F6 + 0 60 moveto ( ) show +F0 + C100 + F6 + 0 70 moveto (Turn Predictions) show +F0 + C100 + F6 + 0 80 moveto ( ) show +F0 + C100 + F6 + 0 90 moveto ( ) show +F0 + C100 + F6 + 0 100 moveto ( ) show +F0 + C100 + F6 + 0 110 moveto ( ) show +F0 + C100 + F6 + 0 120 moveto ( ) show +F0 + C100 + F6 + 0 130 moveto ( ) show +F0 + C100 + F6 + 0 140 moveto ( ) show +F0 + C100 + F6 + 0 150 moveto ( ) show +F0 + C100 + F6 + 0 160 moveto ( ) show +F0 + C100 + F6 + 0 170 moveto (Sheet Predictions) show +F0 + C100 + F6 + 0 180 moveto ( ) show +F0 + C100 + F6 + 0 190 moveto ( ) show +F0 + C100 + F6 + 0 200 moveto ( ) show +F0 + C100 + F6 + 0 210 moveto ( ) show +F0 + C100 + F6 + 0 220 moveto ( ) show +F0 + C100 + F6 + 0 230 moveto ( ) show +F0 + C100 + F6 + 0 240 moveto ( ) show +F0 + C100 + F6 + 0 250 moveto ( ) show +F0 + C100 + F6 + 0 260 moveto (Helix Predictions) show +F0 + C100 + F6 + 0 270 moveto ( ) show +F0 + C100 + F6 + 0 280 moveto ( ) show +F0 + C100 + F6 + 0 290 moveto ( ) show +F0 + C100 + F6 + 0 300 moveto ( ) show +F0 + C100 + F6 + 0 310 moveto ( ) show +F0 + C100 + F6 + 0 320 moveto ( ) show +F0 + C100 + F6 + 0 330 moveto ( ) show +F0 + C100 + F6 + 0 340 moveto ( ) show +F0 + C100 + F6 + 0 350 moveto (Conservation +) show +F0 + C100 + F6 + 0 360 moveto ( ) show +F0 + C100 + F6 + 0 370 moveto (Annexin VII 3) show +F0 + C100 + F6 + 0 380 moveto (Annexin III R3) show +F0 + C100 + F6 + 0 390 moveto (Annexin III H3) show +F0 + C100 + F6 + 0 400 moveto (Annexin V BH3) show +F0 + C100 + F6 + 0 410 moveto (Annexin IV P3) show +F0 + C100 + F6 + 0 420 moveto (Annexin IV B3) show +F0 + C100 + F6 + 0 430 moveto (Annexin IV H3) show +F0 + C100 + F6 + 0 440 moveto (Annexin VI M3) show +F0 + C100 + F6 + 0 450 moveto (Annexin VI H3) show +F0 + C100 + F6 + 0 460 moveto (Annexin V R3) show +F0 + C100 + F6 + 0 470 moveto (Annexin V H3) show +F0 + C100 + F6 + 0 480 moveto (Annexin V C3) show +F0 + C100 + F6 + 0 490 moveto (Annexin I R3) show +F0 + C100 + F6 + 0 500 moveto (Annexin I M3) show +F0 + C100 + F6 + 0 510 moveto (Annexin I H3) show +F0 + C100 + F6 + 0 520 moveto (Annexin II B3) show +F0 + C100 + F6 + 0 530 moveto (Annexin II H3) show +F0 + C100 + F6 + 0 540 moveto (Annexin II M3) show +F0 + C100 + F6 + 0 550 moveto (Annexin III R4) show +F0 + C100 + F6 + 0 560 moveto (Annexin III H4) show +F0 + C100 + F6 + 0 570 moveto (Annexin VII 4) show +F0 + C100 + F6 + 0 580 moveto (Annexin V BH4) show +F0 + C100 + F6 + 0 590 moveto (Annexin VI M8) show +F0 + C100 + F6 + 0 600 moveto (Annexin VI H8) show +F0 + C100 + F6 + 0 610 moveto (Annexin VI M4) show +F0 + C100 + F6 + 0 620 moveto (Annexin VI H4) show +F0 + C100 + F6 + 0 630 moveto (Annexin IV H4) show +F0 + C100 + F6 + 0 640 moveto (Annexin IV B4) show +F0 + C100 + F6 + 0 650 moveto (Annexin IV P4) show +F0 + C100 + F6 + 0 660 moveto (Annexin II M4) show +F0 + C100 + F6 + 0 670 moveto (Annexin II B4) show +F0 + C100 + F6 + 0 680 moveto (Annexin II H4) show +F0 + C100 + F6 + 0 690 moveto (Annexin V C4) show +F0 + C100 + F6 + 0 700 moveto (Annexin V H4) show +F0 + C100 + F6 + 0 710 moveto (Annexin V R4) show +F0 + C100 + F6 + 0 720 moveto (Annexin I R4) show +F0 + C100 + F6 + 0 730 moveto (Annexin I M4) show +F0 + C100 + F6 + 0 740 moveto (Annexin I H4) show +F0 + C100 + (H) 96 0 P +(H) 108 0 P +(H) 120 0 P +(H) 132 0 P +(H) 144 0 P +(H) 156 0 P +(H) 168 0 P +(H) 180 0 P +(H) 204 0 P +(H) 216 0 P +( +) 228 0 P +(T) 264 0 P +(T) 276 0 P +(T) 288 0 P +(Y) 300 0 P +(H) 312 0 P +(H) 324 0 P +(H) 336 0 P +(H) 348 0 P +(H) 360 0 P +(H) 372 0 P +(H) 384 0 P +(T) 396 0 P +(T) 408 0 P + 108 330 moveto (Helix Pattern) show + 228 330 moveto (Glycine Loop) show + 336 330 moveto (Helix Pattern) show +(7) 108 350 P +(+) 144 350 P +(7) 180 350 P +(+) 252 350 P +(5) 264 350 P +(6) 324 350 P +(5) 336 350 P +(5) 360 350 P +(6) 372 350 P +(6) 384 350 P +(+) 408 350 P + 1.00 setgray +(D) 96 370 P + 0.00 setgray +F1 + (A) 108 370 P +F0 + (Q) 120 370 P +F4 + (R) 132 370 P +F0 + C1 + (L) 144 370 P +C100 + (Y) 156 370 P +(Q) 168 370 P +F1 + (A) 180 370 P +F0 + (G) 192 370 P + 1.00 setgray +(E) 204 370 P + 0.00 setgray +(G) 216 370 P +F4 + (R) 228 370 P +F0 + C1 + (L) 240 370 P +C100 + (G) 252 370 P +(T) 264 370 P + 1.00 setgray +(D) 276 370 P + 0.00 setgray + 1.00 setgray +(E) 288 370 P + 0.00 setgray +F1 + (S) 300 370 P +F0 + (C) 312 370 P +(F) 324 370 P +F1 + (N) 336 370 P +F0 + (M) 348 370 P +F5 + C1 + (I) 360 370 P +F0 + (L) 372 370 P +F1 + C100 + (A) 384 370 P +F0 + (T) 396 370 P +F4 + (R) 408 370 P +F0 + 1.00 setgray +(D) 96 380 P + 0.00 setgray +F1 + (A) 108 380 P +F0 + (Q) 120 380 P +(T) 132 380 P +C1 + (L) 144 380 P +C100 + (Y) 156 380 P + 1.00 setgray +(D) 168 380 P + 0.00 setgray +F1 + (A) 180 380 P +F0 + (G) 192 380 P + 1.00 setgray +(E) 204 380 P + 0.00 setgray +F4 + (K) 216 380 P +F0 + F4 + (K) 228 380 P +F0 + (W) 240 380 P +(G) 252 380 P +(T) 264 380 P + 1.00 setgray +(D) 276 380 P + 0.00 setgray + 1.00 setgray +(E) 288 380 P + 0.00 setgray + 1.00 setgray +(D) 300 380 P + 0.00 setgray +F4 + (K) 312 380 P +F0 + (F) 324 380 P +(T) 336 380 P + 1.00 setgray +(E) 348 380 P + 0.00 setgray +F5 + C1 + (I) 360 380 P +F0 + (L) 372 380 P +C100 + (C) 384 380 P +C1 + (L) 396 380 P +F4 + C100 + (R) 408 380 P +F0 + 1.00 setgray +(D) 96 390 P + 0.00 setgray +F1 + (A) 108 390 P +F0 + (Q) 120 390 P +F5 + C1 + (I) 132 390 P +F0 + (L) 144 390 P +C100 + (Y) 156 390 P +F4 + (K) 168 390 P +F0 + F1 + (A) 180 390 P +F0 + (G) 192 390 P + 1.00 setgray +(E) 204 390 P + 0.00 setgray +F1 + (N) 216 390 P +F0 + F4 + (R) 228 390 P +F0 + (W) 240 390 P +(G) 252 390 P +(T) 264 390 P + 1.00 setgray +(D) 276 390 P + 0.00 setgray + 1.00 setgray +(E) 288 390 P + 0.00 setgray + 1.00 setgray +(D) 300 390 P + 0.00 setgray +F4 + (K) 312 390 P +F0 + (F) 324 390 P +(T) 336 390 P + 1.00 setgray +(E) 348 390 P + 0.00 setgray +F5 + C1 + (I) 360 390 P +F0 + (L) 372 390 P +C100 + (C) 384 390 P +C1 + (L) 396 390 P +F4 + C100 + (R) 408 390 P +F0 + 1.00 setgray +(D) 96 400 P + 0.00 setgray +F1 + (A) 108 400 P +F0 + (Q) 120 400 P + 1.00 setgray +(D) 132 400 P + 0.00 setgray +C1 + (L) 144 400 P +C100 + (Y) 156 400 P +F1 + (A) 168 400 P +F0 + F1 + (A) 180 400 P +F0 + (G) 192 400 P + 1.00 setgray +(E) 204 400 P + 0.00 setgray +F4 + (K) 216 400 P +F0 + F5 + C1 + (I) 228 400 P +F0 + F4 + C100 + (R) 240 400 P +F0 + (G) 252 400 P +(T) 264 400 P + 1.00 setgray +(D) 276 400 P + 0.00 setgray + 1.00 setgray +(E) 288 400 P + 0.00 setgray +(M) 300 400 P +F4 + (K) 312 400 P +F0 + (F) 324 400 P +F5 + C1 + (I) 336 400 P +F0 + C100 + (T) 348 400 P +F5 + C1 + (I) 360 400 P +F0 + (L) 372 400 P +C100 + (C) 384 400 P +(T) 396 400 P +F4 + (R) 408 400 P +F0 + 1.00 setgray +(D) 96 410 P + 0.00 setgray +F1 + (A) 108 410 P +F0 + (Q) 120 410 P + 1.00 setgray +(D) 132 410 P + 0.00 setgray +C1 + (L) 144 410 P +C100 + (Y) 156 410 P + 1.00 setgray +(E) 168 410 P + 0.00 setgray +F1 + (A) 180 410 P +F0 + (G) 192 410 P + 1.00 setgray +(E) 204 410 P + 0.00 setgray +F4 + (K) 216 410 P +F0 + F4 + (K) 228 410 P +F0 + (W) 240 410 P +(G) 252 410 P +(T) 264 410 P + 1.00 setgray +(D) 276 410 P + 0.00 setgray + 1.00 setgray +(E) 288 410 P + 0.00 setgray +F5 + C1 + (V) 300 410 P +F0 + F4 + C100 + (K) 312 410 P +F0 + (F) 324 410 P +C1 + (L) 336 410 P +C100 + (T) 348 410 P +F5 + C1 + (V) 360 410 P +F0 + (L) 372 410 P +C100 + (C) 384 410 P +F1 + (S) 396 410 P +F0 + F4 + (R) 408 410 P +F0 + 1.00 setgray +(D) 96 420 P + 0.00 setgray +F1 + (A) 108 420 P +F0 + (Q) 120 420 P + 1.00 setgray +(D) 132 420 P + 0.00 setgray +C1 + (L) 144 420 P +C100 + (Y) 156 420 P + 1.00 setgray +(E) 168 420 P + 0.00 setgray +F1 + (A) 180 420 P +F0 + (G) 192 420 P + 1.00 setgray +(E) 204 420 P + 0.00 setgray +F4 + (K) 216 420 P +F0 + F4 + (K) 228 420 P +F0 + (W) 240 420 P +(G) 252 420 P +(T) 264 420 P + 1.00 setgray +(D) 276 420 P + 0.00 setgray + 1.00 setgray +(E) 288 420 P + 0.00 setgray +F5 + C1 + (V) 300 420 P +F0 + F4 + C100 + (K) 312 420 P +F0 + (F) 324 420 P +C1 + (L) 336 420 P +C100 + (T) 348 420 P +F5 + C1 + (V) 360 420 P +F0 + (L) 372 420 P +C100 + (C) 384 420 P +F1 + (S) 396 420 P +F0 + F4 + (R) 408 420 P +F0 + 1.00 setgray +(D) 96 430 P + 0.00 setgray +F1 + (A) 108 430 P +F0 + (Q) 120 430 P + 1.00 setgray +(D) 132 430 P + 0.00 setgray +C1 + (L) 144 430 P +C100 + (Y) 156 430 P + 1.00 setgray +(E) 168 430 P + 0.00 setgray +F1 + (A) 180 430 P +F0 + (G) 192 430 P + 1.00 setgray +(E) 204 430 P + 0.00 setgray +F4 + (K) 216 430 P +F0 + F4 + (K) 228 430 P +F0 + (W) 240 430 P +(G) 252 430 P +(T) 264 430 P + 1.00 setgray +(D) 276 430 P + 0.00 setgray + 1.00 setgray +(E) 288 430 P + 0.00 setgray +F5 + C1 + (V) 300 430 P +F0 + F4 + C100 + (K) 312 430 P +F0 + (F) 324 430 P +C1 + (L) 336 430 P +C100 + (T) 348 430 P +F5 + C1 + (V) 360 430 P +F0 + (L) 372 430 P +C100 + (C) 384 430 P +F1 + (S) 396 430 P +F0 + F4 + (R) 408 430 P +F0 + 1.00 setgray +(D) 96 440 P + 0.00 setgray +F5 + C1 + (V) 108 440 P +F0 + C100 + (Q) 120 440 P + 1.00 setgray +(D) 132 440 P + 0.00 setgray +C1 + (L) 144 440 P +C100 + (Y) 156 440 P + 1.00 setgray +(E) 168 440 P + 0.00 setgray +F1 + (A) 180 440 P +F0 + (G) 192 440 P + 1.00 setgray +(E) 204 440 P + 0.00 setgray +C1 + (L) 216 440 P +F4 + C100 + (K) 228 440 P +F0 + (W) 240 440 P +(G) 252 440 P +(T) 264 440 P + 1.00 setgray +(D) 276 440 P + 0.00 setgray + 1.00 setgray +(E) 288 440 P + 0.00 setgray +F1 + (A) 300 440 P +F0 + (Q) 312 440 P +(F) 324 440 P +F5 + C1 + (I) 336 440 P +F0 + C100 + (Y) 348 440 P +F5 + C1 + (I) 360 440 P +F0 + (L) 372 440 P +C100 + (G) 384 440 P +F1 + (N) 396 440 P +F0 + F4 + (R) 408 440 P +F0 + 1.00 setgray +(D) 96 450 P + 0.00 setgray +F5 + C1 + (V) 108 450 P +F0 + C100 + (Q) 120 450 P + 1.00 setgray +(D) 132 450 P + 0.00 setgray +C1 + (L) 144 450 P +C100 + (Y) 156 450 P + 1.00 setgray +(E) 168 450 P + 0.00 setgray +F1 + (A) 180 450 P +F0 + (G) 192 450 P + 1.00 setgray +(E) 204 450 P + 0.00 setgray +C1 + (L) 216 450 P +F4 + C100 + (K) 228 450 P +F0 + (W) 240 450 P +(G) 252 450 P +(T) 264 450 P + 1.00 setgray +(D) 276 450 P + 0.00 setgray + 1.00 setgray +(E) 288 450 P + 0.00 setgray +F1 + (A) 300 450 P +F0 + (Q) 312 450 P +(F) 324 450 P +F5 + C1 + (I) 336 450 P +F0 + C100 + (Y) 348 450 P +F5 + C1 + (I) 360 450 P +F0 + (L) 372 450 P +C100 + (G) 384 450 P +F1 + (N) 396 450 P +F0 + F4 + (R) 408 450 P +F0 + 1.00 setgray +(D) 96 460 P + 0.00 setgray +F1 + (A) 108 460 P +F0 + (Q) 120 460 P +F1 + (A) 132 460 P +F0 + C1 + (L) 144 460 P +C100 + (F) 156 460 P +(Q) 168 460 P +F1 + (A) 180 460 P +F0 + (G) 192 460 P + 1.00 setgray +(E) 204 460 P + 0.00 setgray +C1 + (L) 216 460 P +F4 + C100 + (K) 228 460 P +F0 + (W) 240 460 P +(G) 252 460 P +(T) 264 460 P + 1.00 setgray +(D) 276 460 P + 0.00 setgray + 1.00 setgray +(E) 288 460 P + 0.00 setgray + 1.00 setgray +(E) 300 460 P + 0.00 setgray +F4 + (K) 312 460 P +F0 + (F) 324 460 P +F5 + C1 + (I) 336 460 P +F0 + C100 + (T) 348 460 P +F5 + C1 + (I) 360 460 P +F0 + (L) 372 460 P +C100 + (G) 384 460 P +(T) 396 460 P +F4 + (R) 408 460 P +F0 + 1.00 setgray +(D) 96 470 P + 0.00 setgray +F1 + (A) 108 470 P +F0 + (Q) 120 470 P +F1 + (A) 132 470 P +F0 + C1 + (L) 144 470 P +C100 + (F) 156 470 P +(Q) 168 470 P +F1 + (A) 180 470 P +F0 + (G) 192 470 P + 1.00 setgray +(E) 204 470 P + 0.00 setgray +C1 + (L) 216 470 P +F4 + C100 + (K) 228 470 P +F0 + (W) 240 470 P +(G) 252 470 P +(T) 264 470 P + 1.00 setgray +(D) 276 470 P + 0.00 setgray + 1.00 setgray +(E) 288 470 P + 0.00 setgray + 1.00 setgray +(E) 300 470 P + 0.00 setgray +F4 + (K) 312 470 P +F0 + (F) 324 470 P +F5 + C1 + (I) 336 470 P +F0 + C100 + (T) 348 470 P +F5 + C1 + (I) 360 470 P +F0 + C100 + (F) 372 470 P +(G) 384 470 P +(T) 396 470 P +F4 + (R) 408 470 P +F0 + 1.00 setgray +(D) 96 480 P + 0.00 setgray +F1 + (A) 108 480 P +F0 + (Q) 120 480 P +F5 + C1 + (V) 132 480 P +F0 + (L) 144 480 P +C100 + (F) 156 480 P +F4 + (R) 168 480 P +F0 + F1 + (A) 180 480 P +F0 + (G) 192 480 P + 1.00 setgray +(E) 204 480 P + 0.00 setgray +C1 + (L) 216 480 P +F4 + C100 + (K) 228 480 P +F0 + (W) 240 480 P +(G) 252 480 P +(T) 264 480 P + 1.00 setgray +(D) 276 480 P + 0.00 setgray + 1.00 setgray +(E) 288 480 P + 0.00 setgray + 1.00 setgray +(E) 300 480 P + 0.00 setgray +(T) 312 480 P +(F) 324 480 P +F5 + C1 + (I) 336 480 P +F0 + C100 + (T) 348 480 P +F5 + C1 + (I) 360 480 P +F0 + (L) 372 480 P +C100 + (G) 384 480 P +(T) 396 480 P +F4 + (R) 408 480 P +F0 + 1.00 setgray +(D) 96 490 P + 0.00 setgray +F1 + (A) 108 490 P +F0 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730 P + 0.00 setgray +F4 + (K) 132 730 P +F0 + C1 + (L) 144 730 P +C100 + (Y) 156 730 P + 1.00 setgray +(E) 168 730 P + 0.00 setgray +F1 + (A) 180 730 P +F0 + (M) 204 730 P +F4 + (K) 216 730 P +F0 + (G) 228 730 P +F1 + (A) 240 730 P +F0 + (G) 252 730 P +(T) 264 730 P +F4 + (R) 276 730 P +F0 + (H) 288 730 P +F4 + (K) 300 730 P +F0 + F1 + (A) 312 730 P +F0 + C1 + (L) 324 730 P +F5 + (I) 336 730 P +F0 + F4 + C100 + (R) 348 730 P +F0 + F5 + C1 + (I) 360 730 P +F0 + C100 + (M) 372 730 P +F5 + C1 + (V) 384 730 P +F0 + F1 + C100 + (S) 396 730 P +F0 + F4 + (R) 408 730 P +F0 + (F) 96 740 P +F1 + (A) 108 740 P +F0 + 1.00 setgray +(E) 120 740 P + 0.00 setgray +F4 + (K) 132 740 P +F0 + C1 + (L) 144 740 P +C100 + (H) 156 740 P +(Q) 168 740 P +F1 + (A) 180 740 P +F0 + (M) 204 740 P +F4 + (K) 216 740 P +F0 + (G) 228 740 P +F5 + C1 + (V) 240 740 P +F0 + C100 + (G) 252 740 P +(T) 264 740 P +F4 + (R) 276 740 P +F0 + (H) 288 740 P +F4 + (K) 300 740 P +F0 + F1 + (A) 312 740 P +F0 + C1 + (L) 324 740 P +F5 + (I) 336 740 P +F0 + F4 + C100 + (R) 348 740 P +F0 + F5 + C1 + (I) 360 740 P +F0 + C100 + (M) 372 740 P +F5 + C1 + (V) 384 740 P +F0 + F1 + C100 + (S) 396 740 P +F0 + F4 + (R) 408 740 P +F0 + C100 + 96 750 moveto (1) show + 204 750 moveto (10) show + 324 750 moveto (20) show +showpage diff --git a/sources/alscript/examples/demo/example4.als b/sources/alscript/examples/demo/example4.als new file mode 100644 index 0000000..b9a2c77 --- /dev/null +++ b/sources/alscript/examples/demo/example4.als @@ -0,0 +1,192 @@ +# Illustration of ALSCRIPT Command File +# +# Illustrates some of the MASK commands +# +# example 1 # Comment lines begin with the # symbol +# +# +# (A) Input and output files: +# +BLOCK_FILE example1.blc # Define the block file to format +OUTPUT_FILE example4.ps # Where to put the result +# +MAX_SIDE 11 # Fiddle with margins... +Y_OFFSET 50 # This is just to fill the page with the +X_OFFSET 80 # Alignment. Defaults normally work OK +# +# (B) Page Layout and overall spacing: +# +ADD_SEQ 38 1 # Make space after sequence 38 +ADD_SEQ 39 3 # Make space after sequence 39 +ADD_SEQ 69 1 +PORTRAIT # Portrait paper orientation +POINTSIZE 10 # 10 point default pointsize +#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot +IDENT_WIDTH 8 +# +# (C) Font definitions: +# +DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize +DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT +DEFINE_FONT 4 Times-Bold DEFAULT +DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT +DEFINE_FONT 6 Times-Roman DEFAULT +SETUP # Tell the program to get on with the formatting +# +# some formatting commands +# +SURROUND_CHARS LIV ALL # Surround all L and Y characters +FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters +FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps +FONT_CHARS KR ALL 4 # Show K and R in Times-Bold +BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 +SHADE_CHARS + ALL 0.0 # Shade all + characters black +SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 +LINE TOP 2 40 8 # Draw horizontal lines to +LINE BOTTOM 2 40 8 # Join up surrounded numbers +TEXT 2 42 "Helix Pattern" +TEXT 12 42 "Glycine Loop" # Add text annotations +TEXT 21 42 "Helix Pattern" +SUB_ID 49 "Helix Predictions" +SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms +SUB_ID 68 "Turn Predictions" +#INVERSE_CHARS DE ALL +#SHADE_CHARS D ALL 0.5 +#SHADE_CHARS E ALL 0.0 +# +# (D) Some formatting commands: to illustrate the use of MASK +# +mask SETUP #setup the mask +sub_chars 1 1 27 38 SPACE . #substitute spaces for dots +mask ILLEGAL "." #. is not to be used in frequency calculation +mask FRE 1 1 27 38 # do the frequency calculation +mask BOX 1 1 27 38 # use the resulting mask to create boxing lines +mask SHADE 1 1 27 38 0.2 # shade +mask INVERSE 1 1 27 38 # inverse - could also alter the font +sub_chars 1 1 27 38 . SPACE # switch the dots back to spaces +# +# (E) Now make the prediction histograms nicer. +# +SURROUND_CHARS H 1 44 27 53 +SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey +SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" +# +SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions +SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions +SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions +# +SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions +SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions +SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions +# +SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction +SHADE_CHARS HY 1 75 27 75 0.5 +SHADE_CHARS T 1 75 27 75 0.9 +# +# +# Now change the identifier codes +# +ID_FONT ALL 6 +SUB_ID 1 "Annexin I H4" +SUB_ID 2 "Annexin I M4" +SUB_ID 3 "Annexin I R4" +SUB_ID 4 "Annexin V R4" +SUB_ID 5 "Annexin V H4" +SUB_ID 6 "Annexin V C4" +SUB_ID 7 "Annexin II H4" +SUB_ID 8 "Annexin II B4" +SUB_ID 9 "Annexin II M4" +SUB_ID 10 "Annexin IV P4" +SUB_ID 11 "Annexin IV B4" +SUB_ID 12 "Annexin IV H4" +SUB_ID 13 "Annexin VI H4" +SUB_ID 14 "Annexin VI M4" +SUB_ID 15 "Annexin VI H8" +SUB_ID 16 "Annexin VI M8" +SUB_ID 17 "Annexin V BH4" +SUB_ID 18 "Annexin VII 4" +SUB_ID 19 "Annexin III H4" +SUB_ID 20 "Annexin III R4" +SUB_ID 21 "Annexin II M3" +SUB_ID 22 "Annexin II H3" +SUB_ID 23 "Annexin II B3" +SUB_ID 24 "Annexin I H3" +SUB_ID 25 "Annexin I M3" +SUB_ID 26 "Annexin I R3" +SUB_ID 27 "Annexin V C3" +SUB_ID 28 "Annexin V H3" +SUB_ID 29 "Annexin V R3" +SUB_ID 30 "Annexin VI H3" +SUB_ID 31 "Annexin VI M3" +SUB_ID 32 "Annexin IV H3" +SUB_ID 33 "Annexin IV B3" +SUB_ID 34 "Annexin IV P3" +SUB_ID 35 "Annexin V BH3" +SUB_ID 36 "Annexin III H3" +SUB_ID 37 "Annexin III R3" +SUB_ID 38 "Annexin VII 3" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/examples/demo/ipns.als b/sources/alscript/examples/demo/ipns.als new file mode 100644 index 0000000..bd33cbf --- /dev/null +++ b/sources/alscript/examples/demo/ipns.als @@ -0,0 +1,307 @@ +# +# This is the alscript command file used to create the figure +# published in: Roach P. L. et al (1995), Nature (June) +# +# The figure shows Isopenicillin N synthase with homologues +# secondary structure and other information. It makes use +# of many features of alscript 2.0 +# +# Comments in the file all start with the # sign +# +# +# define the alignment block file +BLOCK_FILE ipns.blc +# define the output file name +OUTPUT_FILE ipns.ps +# select portrait mode - longest paper side is vertical +PORTRAIT +# select point size for characters - this can be any number. +POINTSIZE 6.5 +# +# Define fonts that we will use in the alignment. You give each font +# a unique number. The names of the fonts are standard PostScript +# font names. +# +# This one means call Helvetica at the Default pointsize, font 0 +DEFINE_FONT 0 Helvetica DEFAULT +# This means call Helvetica-Oblique at 70% of the default point size, font 1 +DEFINE_FONT 1 Helvetica-Oblique REL 0.70 +DEFINE_FONT 2 Helvetica-Bold DEFAULT +define_font 5 Symbol REL 1.2 +define_font 6 Helvetica REL 0.7 +# +# Colour definitions +# +# Define the colours that we will use in the alignment. Each colour is given +# a number by which it is referred to later. The three numbers following +# each colour are red,green,blue intensities. +# +# e.g. this defines colour number 1 to be red (1,0,0). +DEFINE_COLOUR 1 1 0 0 +#DEFINE_COLOUR 2 0.8 1 0.8 +DEFINE_COLOUR 2 1 1 0 +DEFINE_COLOUR 3 0 0 1 +DEFINE_COLOUR 4 1 1 1 +DEFINE_COLOUR 5 1 1 1 +DEFINE_COLOUR 6 1 0.5 0 +DEFINE_COLOUR 7 1 0 1 +DEFINE_COLOUR 8 0 0 0 +DEFINE_COLOUR 9 1 1 1 +define_colour 10 0 1 0 +define_colour 11 0 0 0 +# +# The add_seq command adds extra space to the alignment you have read in. +# +# +# Here we are adding space for four more lines after sequence number 8. +add_seq 8 4 +# +# This means don't number the sequences +no_numbers +# +# Alter the width of the space for identifiers at the lhs of the plot. This +# must be set by trial and error - 10 is the default. +ident_width 6 +# +# Only print sequence identifiers on the first line of a multi-line/page +# alignment plot. +id_only_on_first_line +# +# This is some fiddling to reduce the default vertical spacing between +# sequence lines. We did this to improve the appearance of the alignment +# in a single column of Nature. Other features of the display can +# be modified in a similar way - e.g. x_space_factor. +y_space_factor 0.05 +# +# commands above the SETUP are about the overall layout etc. Commands +# after, are giving specific boxing/shading/colouring etc. +# +SETUP +# +# Calcluate conservation values for the complete alignment. Conservation +# values range from 0-10 where 10 is an identity. Values above 5 show +# increasing similarities in physico-chemical properties. Having calculated +# conservation values, we can then colour the plot to highlight conserved +# positions. +# +calcons 1 1 424 8 +# +# set mask for conserved position better than 5 +# +# this clears the MASK array of all previous settings +mask SETUP +# this masks all columns of the alignment for which the conservation +# is > 5. +mask CONSERVATION ALL 5 +# +# Now we box the masked positions and colour the +# text backgrounds yellow (colour 2). Note that this operation could +# be restricted to a subset of the alignment rather than the whole thing. +mask BOX 1 1 424 8 +# +# The SCOL command colours the backgrounds of the character. The CCOL +# command colours the character itself. +mask SCOL 1 1 424 8 2 +# +# This resets the MASK commands ready for another operation. If you don't +# reset, then subsequent mask commands are ADDED to the existing mask +# +mask RESET +# +#now do thw same for identities, only this time in red +#with white lettering +mask CONSERVATION ALL 10 +mask SCOL 1 1 424 8 1 +mask CCOL 1 1 424 8 99 +mask FONT 1 1 424 8 2 +mask RESET +# +# sub_id allows the identifier to be modified. At present it is not possible +# to mix fonts within an ID, but the ID_FONT command can be used to modify the +# font used for individual IDs. +sub_id 1 "ACCO" +sub_id 2 "F3OH" +sub_id 3 "H6H " +sub_id 4 "FOLS" +sub_id 5 "GC20" +sub_id 6 "DAOCS" +sub_id 7 "DAOC/DACS " +sub_id 8 "IPNS" +# +# These are the secondary structure definitions. They were entered by +# hand from an analysis of the IPNS structure. +# +# Secondary structure +# +# Set Numbering relative to IPNS +# +# The x values (sequence position) of any commands that follow a +# relative_to command will be translated into absolute alignment position. +# This takes account of gaps in the alignment. +relative_to 8 +# +# Set the font for the line that will say alpha1, beta2 etc to Symbol font. +# Region commands operate on a region of the alignment - they usually expect +# four numbers to define the region - the numbers are x,y,x1,y1 where y and y1 +# are the sequences and x and x1 are the residue positions. +font_region 1 11 331 11 5 +# +#colour all secondary structures black (colour 11) +# +colour_text_region 1 10 331 10 11 +# +# Now draw all the helices and strands and label and colour them. +# +# For example, Tell ALSCRIPT to draw a strand from 11 to 13 of sequence 10 +strand 11 10 13 +# Now write the text b1 (will be a Greek b because of the +# font_region command above). + text 12 11 "b1" +helix 15 10 18 + text 16 11 "a1" +helix 22 10 37 + text 30 11 "a2" +strand 41 10 45 + text 43 11 "b2" +helix 51 10 64 + text 57 11 "a3" +helix 67 10 73 + text 70 11 "a4" +strand 89 10 91 + text 90 11 "b3" +strand 101 10 105 + text 103 11 "b4" +helix 115 10 118 + text 116 11 "a5" +helix 138 10 163 + text 151 11 "a6" +helix 172 10 174 + text 173 11 "a7" +# +#barrel strand 1 +strand 183 10 189 +# set the colour to green for this strand. + colour_text_region 183 10 189 10 10 + text 186 11 "b5" +# +helix 197 10 199 + text 198 11 "a8" +strand 201 10 202 + text 201 11 "b6" +strand 208 10 209 + text 208 11 "b7" +# +#barrel strands 2-6 +strand 210 10 214 + text 212 11 "b8" + colour_text_region 210 10 214 10 10 +strand 220 10 225 + text 222 11 "b9" + colour_text_region 220 10 225 10 10 +strand 231 10 235 + text 233 11 "b10" + colour_text_region 231 10 235 10 10 +strand 238 10 241 + text 239 11 "b11" + colour_text_region 238 10 241 10 10 +strand 249 10 253 + text 250 11 "b12" + colour_text_region 249 10 253 10 10 +# +helix 255 10 260 + text 257 11 "a9" +# +# +#barrel strands 7-8 +strand 270 10 273 + text 271 11 "b13" + colour_text_region 270 10 273 10 10 +strand 279 10 286 + text 282 11 "b14" + colour_text_region 279 10 286 10 10 +# +# +# +strand 293 10 294 + text 293 11 "b15" +strand 311 10 312 + text 311 11 "b16" +helix 313 10 323 + text 317 11 "a10" +# +#Now add arrows for the residues within 8A of the Mn +# +# The TEXT command normally just writes out the specified text +# at the specified position. However, special TEXT objects +# exist. These all start with the @ symbol. +# +colour_text_region 214 12 214 12 1 +# +# This draws a filled up arrow at position 214,12. +text 214 12 "@fuparrow" +colour_text_region 216 12 216 12 1 +text 216 12 "@fuparrow" +colour_text_region 270 12 270 12 1 +text 270 12 "@fuparrow" +colour_text_region 330 12 330 12 1 +text 330 12 "@fuparrow" +# +text 41 12 "@fuparrow" +text 252 12 "@fuparrow" +text 221 12 "@fuparrow" +text 187 12 "@fuparrow" +text 285 12 "@fuparrow" +text 223 12 "@fuparrow" +text 272 12 "@fuparrow" +text 218 12 "@fuparrow" +text 231 12 "@fuparrow" +# +# +# Now set the sequence numbering back to alignment positions. +relative_to 0 +# +# +# From here to the end of the file was created by the alsnum program +# alsnum creates text commands for numbering a plot relative to a +# specific sequence, rather than the alignment numbers that are +# normally shown. Please see the ALSCRIPT manual for further details. +# +# +# Text commands created for numbering +# Sequence Number: 8 +# Starting Number: 1 +# Interval: 10 +# Position: 9 +TEXT 53 9 "10" +TEXT 65 9 "20" +TEXT 78 9 "30" +TEXT 88 9 "40" +TEXT 101 9 "50" +TEXT 111 9 "60" +TEXT 121 9 "70" +TEXT 131 9 "80" +TEXT 141 9 "90" +TEXT 151 9 "100" +TEXT 161 9 "110" +TEXT 171 9 "120" +TEXT 181 9 "130" +TEXT 191 9 "140" +TEXT 201 9 "150" +TEXT 211 9 "160" +TEXT 221 9 "170" +TEXT 231 9 "180" +TEXT 241 9 "190" +TEXT 251 9 "200" +TEXT 261 9 "210" +TEXT 271 9 "220" +TEXT 281 9 "230" +TEXT 297 9 "240" +TEXT 307 9 "250" +TEXT 317 9 "260" +TEXT 327 9 "270" +TEXT 343 9 "280" +TEXT 353 9 "290" +TEXT 363 9 "300" +TEXT 378 9 "310" +TEXT 388 9 "320" +TEXT 398 9 "330" diff --git a/sources/alscript/examples/demo/ipns.blc b/sources/alscript/examples/demo/ipns.blc new file mode 100644 index 0000000..9b73e92 --- /dev/null +++ b/sources/alscript/examples/demo/ipns.blc @@ -0,0 +1,438 @@ + +Conversion of CLUSTAL NBRF PIR file to AMPS BLOCKFILE format +clus2blc: Geoffrey J. Barton (1992) + +>P1;3_LYCES 3_LYCES 418 bp PROT 0 JAN 1900, 418 bases, 1CE32436 checksum. +>P1;U04434 U04434 428 bp PROT 0 JAN 1900, 428 bases, A5C97FB7 checksum. +>P1;HY6H_HYONI HY6H_HYONI 406 bp PROT 0 JAN 1900, 406 bases, DF93DD1E checksum. +>P1;S67953 S67953 400 bp PROT 0 JAN 1900, 400 bases, 1276A781 checksum. +>P1;X73314 X73314 428 bp PROT 0 JAN 1900, 428 bases, 860E176 checksum. +>P1;CEFE_STRCL CEFE_STRCL 402 bp PROT 0 JAN 1900, 402 bases, 97CFEE75 checksum. +>P1;EXPA_CEPAC EXPA_CEPAC 422 bp PROT 0 JAN 1900, 422 bases, AE896850 checksum. +>P1;IPNS_EMENI IPNS_EMENI 403 bp PROT 0 JAN 1900, 403 bases, 4DADFBBB checksum. +* iteration 1 + M + A + L + N + MG + KK + TV + M AA + A QT + P GE + V VS + S SA + I AP + S TS + A LN + V TL + P MN + F EE + LAVE + PTAM + TFRK + AVVG + ASQE + ENAY + GWIR + ESAP + TTSP + NKLF + VSSG + RVKG + ASCS + SEMD + FSDE + VFTS + RIIK + EAPV + EPSP + DLEE + EQYD + RKIF + PRRI + KASW + VEES + PKNE M + HDEK G + DVQF S + RPPE V + FVAA S +MSGASDTK +EDNTETSA +NEDTLTKN +FVVLLVVV +PPPHPPPP +IVIGVTVK +IVIVLFFI +NSDVDSRD +LLLLVLLV + P + QT +EEQVIADS +KGQPDEDP +LIHVLLLL +ND IE F + G DK G + LF D + RM D + S + G + D +GP DK Q +DR PS A +ER DYQKA +RRHEVQSK +AAHNEGGM +NELKELKR +TILMAHVV +MRVVTQLA +EGQKRDTQ +MRQLLEEQ +IVIIVFLI +KATADRAD +DAKDEREA +AAAAACAA +CCCSCLVS +EEQKRRTR +NDDEQDTD +WWFWHKKT +GGGGGGGG +FILIILIF +FFFFFFFF +EQQQFYYY +LVVLVLLA +VVIIVTTV +NDNNNDEN +HHHHHCSH +GGGGGGGG +IVFIVLLI +PDPPDTV +HAEDIDD +EAEEETD +VLLAMEDN +MVMIMLHV +DALAGKTQ +TDEDRSSR +VMTLVAAL +EAMQHKRS +KREKDDEQ +MLVVCLTK +TACGMVCT +KRKKNIVK +GDEEEDDE +HFFFFFFF +YFFFFFFH +KAAETEKM +KLLHMHNS +CPPVPGGI +MPAPLSST +EEEQDEEP +QDEEVAEE +RKKEKEEE +FLEKQKKK +KRKERRRW +EFFLAAAD +LDKIKVVL +VMPARTTA +ASKKKSLI +SGGTVPAR +KGEPGVDA + A Y + A N + K K + F E + E H + LG Q + PS PRD + LN TNQ + KEDEMAV + KQISRRR + GKEYRRA +GGAGGGGG +LFKYYFFY +EILGTTSY +AVYTNGAL + SVSSLLS + SELFEEI + G FSWP + E GEEG + RSSK + TTK + AAA + FQVV +VHQQAIVE +QLLKSTTS +AQSENNEF +EGNVLTTC +VEEEPGGY +TAEGWSKL +DVFKKYYN +LQLKESSP +DDYGTDDN +WWWWFYYF +ERKVSSST +SEDDLMTP +TITHRCCD +FVLLCYYH +FTAFVSSP +LYHHAMMR +RFGKAGGI +HSCIQTIQ +LYHWNAGA + PPPSDGK + VLPSNNT +PKDSA P +TAQAA T +SRDVH H +NDLND E +IYVYY V +SSNRV N +QRSYLLLV +VWWWDFFW +PPPPTPPP +DDEKLSND +LKKNGGRE +DPPPPDGT + S K + F H +EAAPS P +EAKSH G +YWYYHFFF +RRRRGEEQ +EAEEKRDD +VVVAAIVF +MVVNYWWA +REAEQTQE +DRKEEQDQ +FYYYCYYY +ASSGGFFY +KEVKIDDW +RQERARRD +LLVMLQMV +EMRRNYYF +KAKEETGG +LLLVLAAL +AATVGSAS +ECMDTRKS +ERRRKADA +LLMIIVVL +LLLFVAAL +DGDKERRK +LVYSLEAG +LLILLVVY +CSCSGLLA +EEELLRNL +NAGGSASA +LMLLLTVL +GGGGGGGG +LLLLITAK +EDKESEPE +KTLGRPLE +GEGHEDAN +YAYEY F +LLFMF F +KADIKGGA +NRNENGER +AAEAFVDH +FCLA F +YVSG K +GD IP + EDD + ADD +S GFFFT +K DELVL +G EDDEA +PMQINCCS +NDIVDEDV +FQQYSPPV +GKMLILLL +TVMLLLLI +KVLKRRRR +VVTILFLY +SNNNNRRP +NFYYYYYY +YYYYYFFL +PPPPPPPD +PRPPTQEP +CCCCCVVY +PPPPDPPP +KQDRKEEE +PPPPPHDA +DDSDERRA +LLSLVSVI + AAK + T + A + A + EED + EEG + QET +ITTAVPPK +KLLLLLLL +GGGGGRRS +LLSVTMMF +RKGVGAGE +ARGAPPPW +HHHHHHHH +TTYTTYYE +DDDDDDDD +APGMPLLV +GGNSTSSS +GTLYSMTL +IIIIVVII +ITTTTTTT +LLLIILLV +LLLLLIVL +FLQVHQHY +QQQPQQQQ +DDDNDTTS +DL EPPAN +K CCV +VVLVVAAQ +SGPQSNNN +GGGGGGG + FF + VV + SS +LLLLLLLL +QQQQQQQQ +LAQVVACV +LTLFCEEE +KRIKSVVT +DDVDNGDA +EGKGDGGA + GD + RA +QTTHQAEG +WWWWWFFY +IIIYYTVQ +DTADSDDD +VVVVILLI +PQQKPPPE +PPPYPYTA +MVIINRLD +REPPPPPD +HGTNEDGT +SAAAAAAG +IFFLFVMY +VVVIVLVL +VVVVIVVI +NNNHNFFN +LLLIICCC +GGGGGGGG +DDLDDAAS +QHTQTIVY +LGLVFAGM +EHKETTTA +VLVISLLH +ILILLVAL +TSTSTTTT +NNNNNGGN +GGEGGGGN +KRKKIQKY +YFFYYVVY +KKEKKKKK +SNGSGAAA +VASVCPPP +LDIYIRKI +HHHHHHHH +RQRRRHRR +VAVTAVVV +IVVTVAKK +AVTVVAS + PP + RG + RR + DD +QNDNNQQ +TSPKSIRW +DETDMAVV +GCRKNGGN +TSDTASSA + SSE +RRRRRRRR +MLVMKTTQ +SSSSSSSS +LIIWLSSL +AAAPAVVP +STTVFFFF +FFLFFFFF +YQILLLLV +NNGECRRN +PPPPPPPL +GADPSNKG +SPYSHAPY +DDSEDDDD +AACHKFFS +VTTEVTSV +IVIVVFFI +YYEGRSND +PPPPAVVP +ALAIPPQF +KAKPELQD +T EKEASP +LVLLLRRR +VRLLVEEE +EDNSECWP +KGQEKGGN +EEDASFFG +AANNPDNK +EPPPPVVS +EIP SR + LL LI +SDY +THK +QPPPR +VIYKKDPD +YTSFYGSR +PFYKPEEE +KASTDTRP +FEEKYATL +VMFKKTTS +FYAYWFFY +DRDKPQRG +DRIDMDED +YKYYLWWY +MMLVLILL +KASYEGGQ +LRDCMGGN +YDKKTNNG +AISLQYYL +GEDNKVVV +LLYKRNNS +KADLYIML +FRSPRRRI +QLGQPRRN +AKV DTDK +KKK CSKN +EQP NKPG +PAY TAAQ +RKK L AT +FAI E A +EDN A E +AKV F A +MK K A +KQ T V +AQ W P +MQ V A +EQ Q A +SS E A +DA G P +PN K V +IK A S +AE L T +SF D A +AA T A + D G P + S S I + K T A + P I T + L T + D A + A P + I S + F A + A +* diff --git a/sources/alscript/examples/demo/ipns.ps b/sources/alscript/examples/demo/ipns.ps new file mode 100644 index 0000000..96ab236 --- /dev/null +++ b/sources/alscript/examples/demo/ipns.ps @@ -0,0 +1,22867 @@ +%!PS-Adobe-2.0 +%%Creator: ALSCRIPT Version 2.04 - 28 June 1996 +%%Title: Alscript Output +% Barton, G. 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point default pointsize +DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica +SETUP #Tell the program to get on with the formatting. + diff --git a/sources/alscript/examples/example.blc b/sources/alscript/examples/example.blc new file mode 100644 index 0000000..30cfdd3 --- /dev/null +++ b/sources/alscript/examples/example.blc @@ -0,0 +1,33 @@ +A simple example block file. The important parts are the lines that +start with the greater than character - these are identifer lines for the +sequences. The first star is above the position of the first sequence +in the alingmnet which is written VERTICALLY down the page. The second +star shows the end of the alignment. + +Anything else can be written here, except greater than signs and stars. + +This is the format produced by the AMPS package for multiple +sequence alignment. + + +>one +>two +>three +>four +>five +* +ABCDE +EDFTD +AAAAA +LLL +PPPPP +GGGGG +SSSSS +KLMNT +TTLPQ +SLNST +TTLSN +LQPPL +LLLLL +TTTTT +* diff --git a/sources/alscript/examples/example1.als b/sources/alscript/examples/example1.als new file mode 100644 index 0000000..96302b7 --- /dev/null +++ b/sources/alscript/examples/example1.als @@ -0,0 +1,184 @@ +#SILENT_MODE +# Illustration of ALSCRIPT Command File +# +# As shown in: Barton, G. J. (1992) +# ALSCRIPT- A tool to format multiple sequence alignments +# Protein Engineering (in press) +# +# example 1 # Comment lines begin with the # symbol +# +# +# (A) Input and output files: +# +BLOCK_FILE example1.blc # Define the block file to format +OUTPUT_FILE example1.ps # Where to put the result +# +MAX_SIDE 11 # Fiddle with margins... +Y_OFFSET 50 # This is just to fill the page with the +X_OFFSET 80 # Alignment. Defaults normally work OK +# +# (B) Page Layout and overall spacing: +# +ADD_SEQ 38 1 # Make space after sequence 38 +ADD_SEQ 39 3 # Make space after sequence 39 +ADD_SEQ 69 1 +PORTRAIT # Portrait paper orientation +POINTSIZE 10 # 10 point default pointsize +#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot +IDENT_WIDTH 8 +# +# (C) Font definitions: +# +DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize +DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT +DEFINE_FONT 4 Times-Bold DEFAULT +DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT +DEFINE_FONT 6 Times-Roman DEFAULT +SETUP # Tell the program to get on with the formatting +# +# (D) Some formatting commands: +# +SURROUND_CHARS LIV ALL # Surround all L and Y characters +FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters +FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps +FONT_CHARS KR ALL 4 # Show K and R in Times-Bold +BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 +SHADE_CHARS + ALL 0.0 # Shade all + characters black +SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 +LINE TOP 2 40 8 # Draw horizontal lines to +LINE BOTTOM 2 40 8 # Join up surrounded numbers +TEXT 2 42 "Helix Pattern" +TEXT 12 42 "Glycine Loop" # Add text annotations +TEXT 21 42 "Helix Pattern" +SUB_ID 49 "Helix Predictions" +SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms +SUB_ID 68 "Turn Predictions" +INVERSE_CHARS DE ALL +SHADE_CHARS D ALL 0.5 +SHADE_CHARS E ALL 0.0 +# +# (E) Now make the prediction histograms nicer. +# +SURROUND_CHARS H 1 44 27 53 +SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey +SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" +# +SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions +SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions +SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions +# +SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions +SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions +SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions +# +SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction +SHADE_CHARS HY 1 75 27 75 0.5 +SHADE_CHARS T 1 75 27 75 0.9 +# +# +# Now change the identifier codes +# +ID_FONT ALL 6 +SUB_ID 1 "Annexin I H4" +SUB_ID 2 "Annexin I M4" +SUB_ID 3 "Annexin I R4" +SUB_ID 4 "Annexin V R4" +SUB_ID 5 "Annexin V H4" +SUB_ID 6 "Annexin V C4" +SUB_ID 7 "Annexin II H4" +SUB_ID 8 "Annexin II B4" +SUB_ID 9 "Annexin II M4" +SUB_ID 10 "Annexin IV P4" +SUB_ID 11 "Annexin IV B4" +SUB_ID 12 "Annexin IV H4" +SUB_ID 13 "Annexin VI H4" +SUB_ID 14 "Annexin VI M4" +SUB_ID 15 "Annexin VI H8" +SUB_ID 16 "Annexin VI M8" +SUB_ID 17 "Annexin V BH4" +SUB_ID 18 "Annexin VII 4" +SUB_ID 19 "Annexin III H4" +SUB_ID 20 "Annexin III R4" +SUB_ID 21 "Annexin II M3" +SUB_ID 22 "Annexin II H3" +SUB_ID 23 "Annexin II B3" +SUB_ID 24 "Annexin I H3" +SUB_ID 25 "Annexin I M3" +SUB_ID 26 "Annexin I R3" +SUB_ID 27 "Annexin V C3" +SUB_ID 28 "Annexin V H3" +SUB_ID 29 "Annexin V R3" +SUB_ID 30 "Annexin VI H3" +SUB_ID 31 "Annexin VI M3" +SUB_ID 32 "Annexin IV H3" +SUB_ID 33 "Annexin IV B3" +SUB_ID 34 "Annexin IV P3" +SUB_ID 35 "Annexin V BH3" +SUB_ID 36 "Annexin III H3" +SUB_ID 37 "Annexin III R3" +SUB_ID 38 "Annexin VII 3" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/examples/example1.blc b/sources/alscript/examples/example1.blc new file mode 100644 index 0000000..517ef70 --- /dev/null +++ b/sources/alscript/examples/example1.blc @@ -0,0 +1,102 @@ +>L1H4 +>L1M4 +>L1R4 +>L5R4 +>L5H4 +>L5C4 +>L2H4 +>L2B4 +>L2M4 +>L4P4 +>L4B4 +>L4H4 +>L6H4 +>L6M4 +>L6H8 +>L6M8 +>L5BH4 +>p474 +>L3H4 +>L3R4 +>L2M3 +>L2H3 +>L2B3 +>L1H3 +>L1M3 +>L1R3 +>L5C3 +>L5H3 +>L5R3 +>L6H3 +>L6M3 +>L4H3 +>L4B3 +>L4P3 +>L5BH3 +>L3H3 +>L3R3 +>p473 +>Conservation +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> +> + * + FFFLLFFFFFFFFFFFFFLLDDDDDDDDDDDDDDDDDD HHHHHHH E TTTTH + AAAAAAAAAAAAAAAAAAAAAAAAAAAAAVVAAAAAAA7 HHHHHHHH TTTH + EEEEEEDDDEEEEEDDEEEGRRRRRRQQQQQQQQQQQQ HHHHHHHH TTH + KKKTTTRRRRRKRRKKRRRREDDAAAVAADDDDDDITR HHHHHHHHH E TTTH + LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLL+ HHHHHHHHH E TTTH + HYYYYYYYYYYYFFYYYYHHYYYYYYFFFYYYYYYYYY HHHHHHHHH E TTH + QEEYYYDDDKKKKKKKYYRQDDDEEERQQEEEEEAKDQ HHHHHHHHH E TTH + AAAAASSSSSSSAASSAAAAAAAAAAAAAAAAAAAAAA7 HHHHHHHH E TTH + GGGGGGGGGGGGGGGGGG H T + MMMMMMMMMMMMMMMMMMLLVVVEEEEEEEEEEEEEEE HHHHHHHH E TTTH + KKKKKKKKKKKKKKKKKKKKKKKRRRLLLLLKKKKNKG HHHHHHH E TTTTH + GGGGGGGGGGGGGGGGGGGGRRRRRRKKKKKKKKIRKR HHH TTTTT + VAAAAAKKKLLLLLAAAAIAKKKKKKWWWWWWWWRWWL HHH TTTTT + GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG+ HH TTTTTTT + TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5 HHHH TTTTTTTTTT + RRRDDDRRRDDDRRDDRDDDDDDDDDDDDDDDDDDDDD HHHHH TTTTTTTTTT + HHHDDDDDDDDDDDEEDDEEVVVVVVEEEEEEEEEEEE HHHHHHHH E TTTTTTTTTT + KKKHHDKKKNDNNNKKGSFFPPPNNNEEEAAVVVMDDS HHHHHHH E TTTTTTTY + AATTTTVVVTTTTTTTTTTTKKKVVVTKKQQKKKKKKC HHHHHHHH EEE TTTTTH + LLLLLLLLLLLLLLLLLLLLWWWFFFFFFFFFFFFFFF6 HHHHHHHH EEEE TTH + IIIIIIIIIIIIIITTIVNNIIINTNIIIIILLLITTN5 HHHHHHH EEEE TH + RRRRRRRRRRRRRRRRRRRRSSSTTTTTTYYTTTTEEM HHHHHHH EEEEE H + IIIVVVIIIVVVIIIVNIIIIIIIIIIIIIIVVVIIII5 HHHHHH EEEEE H + MMMIMMMMMMMMMMMMIVMMMMMLLLLFLLLLLLLLLL6 HHHHHH EEEEE TH + VVVVVVVVVVVVVVVVVVVVTTTTTTGGGGGCCCCCCA6 HHHHH EEEE TTTTH + SSSSSSSSSSSSSSSSSTSSEEETSTTTTNNSSSTLLT HHHH EE TTTTTT + RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR+ HHHH EE TTTTTTTTTT + * + + + diff --git a/sources/alscript/examples/example2.als b/sources/alscript/examples/example2.als new file mode 100644 index 0000000..642a7a2 --- /dev/null +++ b/sources/alscript/examples/example2.als @@ -0,0 +1,24 @@ +# FILE example2.com +# +#Commands are free format - separated by blank, tab or comma characters +# +BLOCK_FILE example.blc #define the block file to format +OUTPUT_FILE example2.ps #where to put the result +LANDSCAPE #landscape paper orientation +POINTSIZE 12 #12 point default pointsize +DEFINE_FONT 0 Helvetica DEFAULT #set font 0 to be Helvetica +DEFINE_FONT 1 Helvetica REL 0.5 #set font 1 to be half sized Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT #set font 3 to be Bold Helvetica +DEFINE_FONT 4 Times-BoldItalic DEFAULT #font 4 is Times-BoldItalic +NUMBER_SEQS #Number the sequences at left hand side +SETUP #Tell the program to get on with the formatting. +# +#step 2 commands come after the SETUP command +# +#Here are some examples... +# +SURROUND_CHARS GP ALL #draw lines around all G and P +SHADE_CHARS ILVW ALL 0.6 #shade all I L V and W with value 0.6 +BOX_REGION 1 1 2 20 0.8 #rectangular box from positions 1 to 2 of sequences 1 to 20 +FONT_CHARS C ALL 3 #Use font 3 (BOLD Helvetica) for C characters +ID_FONT ALL 1 #set identifiers in font 1 diff --git a/sources/alscript/examples/example3.als b/sources/alscript/examples/example3.als new file mode 100644 index 0000000..bb9de1f --- /dev/null +++ b/sources/alscript/examples/example3.als @@ -0,0 +1,209 @@ +# Illustration of ALSCRIPT Command File +# +# As shown in: Barton, G. J. (1992) +# ALSCRIPT- A tool to format multiple sequence alignments +# Protein Engineering (in press) +# +# Modified version - includes colour commands +# +# +# example 1 # Comment lines begin with the # symbol +# +# +# (A) Input and output files: +# +BLOCK_FILE example1.blc # Define the block file to format +OUTPUT_FILE example3.ps # Where to put the result +# +MAX_SIDE 11 # Fiddle with margins... +Y_OFFSET 50 # This is just to fill the page with the +X_OFFSET 80 # Alignment. Defaults normally work OK +# +# (B) Page Layout and overall spacing: +# +ADD_SEQ 38 1 # Make space after sequence 38 +ADD_SEQ 39 3 # Make space after sequence 39 +ADD_SEQ 69 1 +PORTRAIT # Portrait paper orientation +POINTSIZE 10 # 10 point default pointsize +#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot +IDENT_WIDTH 8 +# +# (C) Font definitions: +# +DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize +DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT +DEFINE_FONT 4 Times-Bold DEFAULT +DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT +DEFINE_FONT 6 Times-Roman DEFAULT +# +# COLOUR DEFINITIONS give a colour number, then red, green, blue intensities +# +# red +DEFINE_COLOUR 0 1 0 0 +# green +DEFINE_COLOUR 1 0 1 0 +# blue +DEFINE_COLOUR 2 0 0 1 +# yellow +DEFINE_COLOUR 3 1 1 0 +# magenta +DEFINE_COLOUR 4 1 0 1 +# cyan +DEFINE_COLOUR 5 0 1 1 +# +DEFINE_COLOUR 6 0.7 0.3 0 +#single_page +#background_colour 100 +SETUP # Tell the program to get on with the formatting +#background_region -10 -10 468 792 +# +# (D) Some formatting commands: +# +SURROUND_CHARS LIV ALL # Surround all L and Y characters +FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters +FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps +FONT_CHARS KR ALL 4 # Show K and R in Times-Bold +Ccol_CHARS LIV ALL 1 +Scol_CHARS ASN ALL 0 +BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 +SHADE_CHARS + ALL 0.0 # Shade all + characters black +COLOUR_REGION 14 1 16 38 2 +SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 +LINE TOP 2 40 8 # Draw horizontal lines to +LINE BOTTOM 2 40 8 # Join up surrounded numbers +TEXT 2 42 "Helix Pattern" +TEXT 12 42 "Glycine Loop" # Add text annotations +TEXT 21 42 "Helix Pattern" +SUB_ID 49 "Helix Predictions" +SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms +SUB_ID 68 "Turn Predictions" +INVERSE_CHARS DE ALL +SHADE_CHARS D ALL 0.5 +SHADE_CHARS E ALL 0.0 +# +# (E) Now make the prediction histograms nicer. +# +SURROUND_CHARS H 1 44 27 53 +Scol_CHARS H 1 44 27 53 3 # Shade the Helix predictions 0.5 grey +SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" +# +SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions +Scol_CHARS E 1 54 27 63 4 # Colour beta blue +SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions +# +SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions +Scol_CHARS T 1 64 27 73 6 # Shade the Turn Predictions +SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions +# +SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction +SHADE_CHARS HY 1 75 27 75 0.5 +SHADE_CHARS T 1 75 27 75 0.9 +# +# +# Now change the identifier codes +# +ID_FONT ALL 6 +SUB_ID 1 "Annexin I H4" +SUB_ID 2 "Annexin I M4" +SUB_ID 3 "Annexin I R4" +SUB_ID 4 "Annexin V R4" +SUB_ID 5 "Annexin V H4" +SUB_ID 6 "Annexin V C4" +SUB_ID 7 "Annexin II H4" +SUB_ID 8 "Annexin II B4" +SUB_ID 9 "Annexin II M4" +SUB_ID 10 "Annexin IV P4" +SUB_ID 11 "Annexin IV B4" +SUB_ID 12 "Annexin IV H4" +SUB_ID 13 "Annexin VI H4" +SUB_ID 14 "Annexin VI M4" +SUB_ID 15 "Annexin VI H8" +SUB_ID 16 "Annexin VI M8" +SUB_ID 17 "Annexin V BH4" +SUB_ID 18 "Annexin VII 4" +SUB_ID 19 "Annexin III H4" +SUB_ID 20 "Annexin III R4" +SUB_ID 21 "Annexin II M3" +SUB_ID 22 "Annexin II H3" +SUB_ID 23 "Annexin II B3" +SUB_ID 24 "Annexin I H3" +SUB_ID 25 "Annexin I M3" +SUB_ID 26 "Annexin I R3" +SUB_ID 27 "Annexin V C3" +SUB_ID 28 "Annexin V H3" +SUB_ID 29 "Annexin V R3" +SUB_ID 30 "Annexin VI H3" +SUB_ID 31 "Annexin VI M3" +SUB_ID 32 "Annexin IV H3" +SUB_ID 33 "Annexin IV B3" +SUB_ID 34 "Annexin IV P3" +SUB_ID 35 "Annexin V BH3" +SUB_ID 36 "Annexin III H3" +SUB_ID 37 "Annexin III R3" +SUB_ID 38 "Annexin VII 3" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/examples/example4.als b/sources/alscript/examples/example4.als new file mode 100644 index 0000000..b9a2c77 --- /dev/null +++ b/sources/alscript/examples/example4.als @@ -0,0 +1,192 @@ +# Illustration of ALSCRIPT Command File +# +# Illustrates some of the MASK commands +# +# example 1 # Comment lines begin with the # symbol +# +# +# (A) Input and output files: +# +BLOCK_FILE example1.blc # Define the block file to format +OUTPUT_FILE example4.ps # Where to put the result +# +MAX_SIDE 11 # Fiddle with margins... +Y_OFFSET 50 # This is just to fill the page with the +X_OFFSET 80 # Alignment. Defaults normally work OK +# +# (B) Page Layout and overall spacing: +# +ADD_SEQ 38 1 # Make space after sequence 38 +ADD_SEQ 39 3 # Make space after sequence 39 +ADD_SEQ 69 1 +PORTRAIT # Portrait paper orientation +POINTSIZE 10 # 10 point default pointsize +#NUMBER_SEQS 1 # Set to 1 for numbers at left of plot +IDENT_WIDTH 8 +# +# (C) Font definitions: +# +DEFINE_FONT 0 Helvetica DEFAULT # Set font 0 to be Helvetica at default pointsize +DEFINE_FONT 1 Helvetica REL 0.75 # Set font 1 to be 0.75 times default Helvetica +DEFINE_FONT 3 Helvetica-Bold DEFAULT +DEFINE_FONT 4 Times-Bold DEFAULT +DEFINE_FONT 5 Helvetica-BoldOblique DEFAULT +DEFINE_FONT 6 Times-Roman DEFAULT +SETUP # Tell the program to get on with the formatting +# +# some formatting commands +# +SURROUND_CHARS LIV ALL # Surround all L and Y characters +FONT_CHARS IV ALL 5 # Use font 5 for all I and V characters +FONT_CHARS ASN ALL 1 # Show A and S and N characters in small caps +FONT_CHARS KR ALL 4 # Show K and R in Times-Bold +BOX_REGION 14 1 16 38 # Rectangular box of residues 14-16 +SHADE_CHARS + ALL 0.0 # Shade all + characters black +SURROUND_CHARS 56+789 1 40 27 40 # Box numbers and + on line 40 +LINE TOP 2 40 8 # Draw horizontal lines to +LINE BOTTOM 2 40 8 # Join up surrounded numbers +TEXT 2 42 "Helix Pattern" +TEXT 12 42 "Glycine Loop" # Add text annotations +TEXT 21 42 "Helix Pattern" +SUB_ID 49 "Helix Predictions" +SUB_ID 58 "Sheet Predictions" # Change the identifiers for prediction histograms +SUB_ID 68 "Turn Predictions" +#INVERSE_CHARS DE ALL +#SHADE_CHARS D ALL 0.5 +#SHADE_CHARS E ALL 0.0 +# +# (D) Some formatting commands: to illustrate the use of MASK +# +mask SETUP #setup the mask +sub_chars 1 1 27 38 SPACE . #substitute spaces for dots +mask ILLEGAL "." #. is not to be used in frequency calculation +mask FRE 1 1 27 38 # do the frequency calculation +mask BOX 1 1 27 38 # use the resulting mask to create boxing lines +mask SHADE 1 1 27 38 0.2 # shade +mask INVERSE 1 1 27 38 # inverse - could also alter the font +sub_chars 1 1 27 38 . SPACE # switch the dots back to spaces +# +# (E) Now make the prediction histograms nicer. +# +SURROUND_CHARS H 1 44 27 53 +SHADE_CHARS H 1 44 27 53 0.5 # Shade the Helix predictions 0.5 grey +SUB_CHARS 1 44 27 53 H SPACE # Substitute " " for "H" +# +SURROUND_CHARS E 1 54 27 63 # Surround the Beta Predictions +SHADE_CHARS E 1 54 27 63 0.7 # Shade the Beta Predictions +SUB_CHARS 1 54 27 63 E SPACE # Substitute the E for space in the beta predictions +# +SURROUND_CHARS T 1 64 27 73 # Surround the Turn Predictions +SHADE_CHARS T 1 64 27 73 0.9 # Shade the Turn Predictions +SUB_CHARS 1 64 27 73 T SPACE # Substitute the T for space in the turn predictions +# +SUB_ID 75 "Summary Prediction" # Annotate and shade the summary prediction +SHADE_CHARS HY 1 75 27 75 0.5 +SHADE_CHARS T 1 75 27 75 0.9 +# +# +# Now change the identifier codes +# +ID_FONT ALL 6 +SUB_ID 1 "Annexin I H4" +SUB_ID 2 "Annexin I M4" +SUB_ID 3 "Annexin I R4" +SUB_ID 4 "Annexin V R4" +SUB_ID 5 "Annexin V H4" +SUB_ID 6 "Annexin V C4" +SUB_ID 7 "Annexin II H4" +SUB_ID 8 "Annexin II B4" +SUB_ID 9 "Annexin II M4" +SUB_ID 10 "Annexin IV P4" +SUB_ID 11 "Annexin IV B4" +SUB_ID 12 "Annexin IV H4" +SUB_ID 13 "Annexin VI H4" +SUB_ID 14 "Annexin VI M4" +SUB_ID 15 "Annexin VI H8" +SUB_ID 16 "Annexin VI M8" +SUB_ID 17 "Annexin V BH4" +SUB_ID 18 "Annexin VII 4" +SUB_ID 19 "Annexin III H4" +SUB_ID 20 "Annexin III R4" +SUB_ID 21 "Annexin II M3" +SUB_ID 22 "Annexin II H3" +SUB_ID 23 "Annexin II B3" +SUB_ID 24 "Annexin I H3" +SUB_ID 25 "Annexin I M3" +SUB_ID 26 "Annexin I R3" +SUB_ID 27 "Annexin V C3" +SUB_ID 28 "Annexin V H3" +SUB_ID 29 "Annexin V R3" +SUB_ID 30 "Annexin VI H3" +SUB_ID 31 "Annexin VI M3" +SUB_ID 32 "Annexin IV H3" +SUB_ID 33 "Annexin IV B3" +SUB_ID 34 "Annexin IV P3" +SUB_ID 35 "Annexin V BH3" +SUB_ID 36 "Annexin III H3" +SUB_ID 37 "Annexin III R3" +SUB_ID 38 "Annexin VII 3" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/sources/alscript/examples/ipns.als b/sources/alscript/examples/ipns.als new file mode 100644 index 0000000..bd33cbf --- /dev/null +++ b/sources/alscript/examples/ipns.als @@ -0,0 +1,307 @@ +# +# This is the alscript command file used to create the figure +# published in: Roach P. L. et al (1995), Nature (June) +# +# The figure shows Isopenicillin N synthase with homologues +# secondary structure and other information. It makes use +# of many features of alscript 2.0 +# +# Comments in the file all start with the # sign +# +# +# define the alignment block file +BLOCK_FILE ipns.blc +# define the output file name +OUTPUT_FILE ipns.ps +# select portrait mode - longest paper side is vertical +PORTRAIT +# select point size for characters - this can be any number. +POINTSIZE 6.5 +# +# Define fonts that we will use in the alignment. You give each font +# a unique number. The names of the fonts are standard PostScript +# font names. +# +# This one means call Helvetica at the Default pointsize, font 0 +DEFINE_FONT 0 Helvetica DEFAULT +# This means call Helvetica-Oblique at 70% of the default point size, font 1 +DEFINE_FONT 1 Helvetica-Oblique REL 0.70 +DEFINE_FONT 2 Helvetica-Bold DEFAULT +define_font 5 Symbol REL 1.2 +define_font 6 Helvetica REL 0.7 +# +# Colour definitions +# +# Define the colours that we will use in the alignment. Each colour is given +# a number by which it is referred to later. The three numbers following +# each colour are red,green,blue intensities. +# +# e.g. this defines colour number 1 to be red (1,0,0). +DEFINE_COLOUR 1 1 0 0 +#DEFINE_COLOUR 2 0.8 1 0.8 +DEFINE_COLOUR 2 1 1 0 +DEFINE_COLOUR 3 0 0 1 +DEFINE_COLOUR 4 1 1 1 +DEFINE_COLOUR 5 1 1 1 +DEFINE_COLOUR 6 1 0.5 0 +DEFINE_COLOUR 7 1 0 1 +DEFINE_COLOUR 8 0 0 0 +DEFINE_COLOUR 9 1 1 1 +define_colour 10 0 1 0 +define_colour 11 0 0 0 +# +# The add_seq command adds extra space to the alignment you have read in. +# +# +# Here we are adding space for four more lines after sequence number 8. +add_seq 8 4 +# +# This means don't number the sequences +no_numbers +# +# Alter the width of the space for identifiers at the lhs of the plot. This +# must be set by trial and error - 10 is the default. +ident_width 6 +# +# Only print sequence identifiers on the first line of a multi-line/page +# alignment plot. +id_only_on_first_line +# +# This is some fiddling to reduce the default vertical spacing between +# sequence lines. We did this to improve the appearance of the alignment +# in a single column of Nature. Other features of the display can +# be modified in a similar way - e.g. x_space_factor. +y_space_factor 0.05 +# +# commands above the SETUP are about the overall layout etc. Commands +# after, are giving specific boxing/shading/colouring etc. +# +SETUP +# +# Calcluate conservation values for the complete alignment. Conservation +# values range from 0-10 where 10 is an identity. Values above 5 show +# increasing similarities in physico-chemical properties. Having calculated +# conservation values, we can then colour the plot to highlight conserved +# positions. +# +calcons 1 1 424 8 +# +# set mask for conserved position better than 5 +# +# this clears the MASK array of all previous settings +mask SETUP +# this masks all columns of the alignment for which the conservation +# is > 5. +mask CONSERVATION ALL 5 +# +# Now we box the masked positions and colour the +# text backgrounds yellow (colour 2). Note that this operation could +# be restricted to a subset of the alignment rather than the whole thing. +mask BOX 1 1 424 8 +# +# The SCOL command colours the backgrounds of the character. The CCOL +# command colours the character itself. +mask SCOL 1 1 424 8 2 +# +# This resets the MASK commands ready for another operation. If you don't +# reset, then subsequent mask commands are ADDED to the existing mask +# +mask RESET +# +#now do thw same for identities, only this time in red +#with white lettering +mask CONSERVATION ALL 10 +mask SCOL 1 1 424 8 1 +mask CCOL 1 1 424 8 99 +mask FONT 1 1 424 8 2 +mask RESET +# +# sub_id allows the identifier to be modified. At present it is not possible +# to mix fonts within an ID, but the ID_FONT command can be used to modify the +# font used for individual IDs. +sub_id 1 "ACCO" +sub_id 2 "F3OH" +sub_id 3 "H6H " +sub_id 4 "FOLS" +sub_id 5 "GC20" +sub_id 6 "DAOCS" +sub_id 7 "DAOC/DACS " +sub_id 8 "IPNS" +# +# These are the secondary structure definitions. They were entered by +# hand from an analysis of the IPNS structure. +# +# Secondary structure +# +# Set Numbering relative to IPNS +# +# The x values (sequence position) of any commands that follow a +# relative_to command will be translated into absolute alignment position. +# This takes account of gaps in the alignment. +relative_to 8 +# +# Set the font for the line that will say alpha1, beta2 etc to Symbol font. +# Region commands operate on a region of the alignment - they usually expect +# four numbers to define the region - the numbers are x,y,x1,y1 where y and y1 +# are the sequences and x and x1 are the residue positions. +font_region 1 11 331 11 5 +# +#colour all secondary structures black (colour 11) +# +colour_text_region 1 10 331 10 11 +# +# Now draw all the helices and strands and label and colour them. +# +# For example, Tell ALSCRIPT to draw a strand from 11 to 13 of sequence 10 +strand 11 10 13 +# Now write the text b1 (will be a Greek b because of the +# font_region command above). + text 12 11 "b1" +helix 15 10 18 + text 16 11 "a1" +helix 22 10 37 + text 30 11 "a2" +strand 41 10 45 + text 43 11 "b2" +helix 51 10 64 + text 57 11 "a3" +helix 67 10 73 + text 70 11 "a4" +strand 89 10 91 + text 90 11 "b3" +strand 101 10 105 + text 103 11 "b4" +helix 115 10 118 + text 116 11 "a5" +helix 138 10 163 + text 151 11 "a6" +helix 172 10 174 + text 173 11 "a7" +# +#barrel strand 1 +strand 183 10 189 +# set the colour to green for this strand. + colour_text_region 183 10 189 10 10 + text 186 11 "b5" +# +helix 197 10 199 + text 198 11 "a8" +strand 201 10 202 + text 201 11 "b6" +strand 208 10 209 + text 208 11 "b7" +# +#barrel strands 2-6 +strand 210 10 214 + text 212 11 "b8" + colour_text_region 210 10 214 10 10 +strand 220 10 225 + text 222 11 "b9" + colour_text_region 220 10 225 10 10 +strand 231 10 235 + text 233 11 "b10" + colour_text_region 231 10 235 10 10 +strand 238 10 241 + text 239 11 "b11" + colour_text_region 238 10 241 10 10 +strand 249 10 253 + text 250 11 "b12" + colour_text_region 249 10 253 10 10 +# +helix 255 10 260 + text 257 11 "a9" +# +# +#barrel strands 7-8 +strand 270 10 273 + text 271 11 "b13" + colour_text_region 270 10 273 10 10 +strand 279 10 286 + text 282 11 "b14" + colour_text_region 279 10 286 10 10 +# +# +# +strand 293 10 294 + text 293 11 "b15" +strand 311 10 312 + text 311 11 "b16" +helix 313 10 323 + text 317 11 "a10" +# +#Now add arrows for the residues within 8A of the Mn +# +# The TEXT command normally just writes out the specified text +# at the specified position. However, special TEXT objects +# exist. These all start with the @ symbol. +# +colour_text_region 214 12 214 12 1 +# +# This draws a filled up arrow at position 214,12. +text 214 12 "@fuparrow" +colour_text_region 216 12 216 12 1 +text 216 12 "@fuparrow" +colour_text_region 270 12 270 12 1 +text 270 12 "@fuparrow" +colour_text_region 330 12 330 12 1 +text 330 12 "@fuparrow" +# +text 41 12 "@fuparrow" +text 252 12 "@fuparrow" +text 221 12 "@fuparrow" +text 187 12 "@fuparrow" +text 285 12 "@fuparrow" +text 223 12 "@fuparrow" +text 272 12 "@fuparrow" +text 218 12 "@fuparrow" +text 231 12 "@fuparrow" +# +# +# Now set the sequence numbering back to alignment positions. +relative_to 0 +# +# +# From here to the end of the file was created by the alsnum program +# alsnum creates text commands for numbering a plot relative to a +# specific sequence, rather than the alignment numbers that are +# normally shown. Please see the ALSCRIPT manual for further details. +# +# +# Text commands created for numbering +# Sequence Number: 8 +# Starting Number: 1 +# Interval: 10 +# Position: 9 +TEXT 53 9 "10" +TEXT 65 9 "20" +TEXT 78 9 "30" +TEXT 88 9 "40" +TEXT 101 9 "50" +TEXT 111 9 "60" +TEXT 121 9 "70" +TEXT 131 9 "80" +TEXT 141 9 "90" +TEXT 151 9 "100" +TEXT 161 9 "110" +TEXT 171 9 "120" +TEXT 181 9 "130" +TEXT 191 9 "140" +TEXT 201 9 "150" +TEXT 211 9 "160" +TEXT 221 9 "170" +TEXT 231 9 "180" +TEXT 241 9 "190" +TEXT 251 9 "200" +TEXT 261 9 "210" +TEXT 271 9 "220" +TEXT 281 9 "230" +TEXT 297 9 "240" +TEXT 307 9 "250" +TEXT 317 9 "260" +TEXT 327 9 "270" +TEXT 343 9 "280" +TEXT 353 9 "290" +TEXT 363 9 "300" +TEXT 378 9 "310" +TEXT 388 9 "320" +TEXT 398 9 "330" diff --git a/sources/alscript/examples/ipns.blc b/sources/alscript/examples/ipns.blc new file mode 100644 index 0000000..9b73e92 --- /dev/null +++ b/sources/alscript/examples/ipns.blc @@ -0,0 +1,438 @@ + +Conversion of CLUSTAL NBRF PIR file to AMPS BLOCKFILE format +clus2blc: Geoffrey J. Barton (1992) + +>P1;3_LYCES 3_LYCES 418 bp PROT 0 JAN 1900, 418 bases, 1CE32436 checksum. +>P1;U04434 U04434 428 bp PROT 0 JAN 1900, 428 bases, A5C97FB7 checksum. +>P1;HY6H_HYONI HY6H_HYONI 406 bp PROT 0 JAN 1900, 406 bases, DF93DD1E checksum. +>P1;S67953 S67953 400 bp PROT 0 JAN 1900, 400 bases, 1276A781 checksum. +>P1;X73314 X73314 428 bp PROT 0 JAN 1900, 428 bases, 860E176 checksum. +>P1;CEFE_STRCL CEFE_STRCL 402 bp PROT 0 JAN 1900, 402 bases, 97CFEE75 checksum. +>P1;EXPA_CEPAC EXPA_CEPAC 422 bp PROT 0 JAN 1900, 422 bases, AE896850 checksum. +>P1;IPNS_EMENI IPNS_EMENI 403 bp PROT 0 JAN 1900, 403 bases, 4DADFBBB checksum. +* iteration 1 + M + A + L + N + MG + KK + TV + M AA + A QT + P GE + V VS + S SA + I AP + S TS + A LN + V TL + P MN + F EE + LAVE + PTAM + TFRK + AVVG + ASQE + ENAY + GWIR + ESAP + TTSP + NKLF + VSSG + RVKG + ASCS + SEMD + FSDE + VFTS + RIIK + EAPV + EPSP + DLEE + EQYD + RKIF + PRRI + KASW + VEES + PKNE M + HDEK G + DVQF S + RPPE V + FVAA S +MSGASDTK +EDNTETSA +NEDTLTKN +FVVLLVVV +PPPHPPPP +IVIGVTVK +IVIVLFFI +NSDVDSRD +LLLLVLLV + P + QT +EEQVIADS +KGQPDEDP +LIHVLLLL +ND IE F + G DK G + LF D + RM D + S + G + D +GP DK Q +DR PS A +ER DYQKA +RRHEVQSK +AAHNEGGM +NELKELKR +TILMAHVV +MRVVTQLA +EGQKRDTQ +MRQLLEEQ +IVIIVFLI +KATADRAD +DAKDEREA +AAAAACAA +CCCSCLVS +EEQKRRTR +NDDEQDTD +WWFWHKKT +GGGGGGGG +FILIILIF +FFFFFFFF +EQQQFYYY +LVVLVLLA +VVIIVTTV +NDNNNDEN +HHHHHCSH +GGGGGGGG +IVFIVLLI +PDPPDTV +HAEDIDD +EAEEETD +VLLAMEDN +MVMIMLHV +DALAGKTQ +TDEDRSSR +VMTLVAAL +EAMQHKRS +KREKDDEQ +MLVVCLTK +TACGMVCT +KRKKNIVK +GDEEEDDE +HFFFFFFF +YFFFFFFH +KAAETEKM +KLLHMHNS +CPPVPGGI +MPAPLSST +EEEQDEEP +QDEEVAEE +RKKEKEEE +FLEKQKKK +KRKERRRW +EFFLAAAD +LDKIKVVL +VMPARTTA +ASKKKSLI +SGGTVPAR +KGEPGVDA + A Y + A N + K K + F E + E H + LG Q + PS PRD + LN TNQ + KEDEMAV + KQISRRR + GKEYRRA +GGAGGGGG +LFKYYFFY +EILGTTSY +AVYTNGAL + SVSSLLS + SELFEEI + G FSWP + E GEEG + RSSK + TTK + AAA + FQVV +VHQQAIVE +QLLKSTTS +AQSENNEF +EGNVLTTC +VEEEPGGY +TAEGWSKL +DVFKKYYN 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init_times,last_times, new_times; + +void GJ_init_times(void) +{ + + extern struct tms *t_init, *t_last, *t_new; + extern clock_t init_times, last_times, new_times; + + if(t_init == NULL){ + t_init = (struct tms *) GJmalloc(sizeof(struct tms)); + t_new = (struct tms *) GJmalloc(sizeof(struct tms)); + t_last = (struct tms *) GJmalloc(sizeof(struct tms)); + + init_times = times(t_init); + new_times = init_times; + last_times = init_times; + + copy_tms(t_last,t_init); + copy_tms(t_new,t_init); + } +} + +void copy_tms(struct tms *l,struct tms *r) +{ + l->tms_utime = r->tms_utime; + l->tms_stime = r->tms_stime; + l->tms_cutime = r->tms_cutime; + l->tms_cstime = r->tms_cstime; +} + + +void GJ_show_times(FILE *fp,int action) +{ + /* show the time(s) since init_times was called + if action = 0, print the time since the last call to GJ_init_times + or GJ_show_times. + if action = 1, print as in 0, but add the total time since the + first call to GJ_init_times. + action = 2, print elapsed and total time + + tms_utime, tms_stime, tms_cutime and tms_cstime + */ + + clock_t init_calling, new_calling, last_calling; + clock_t init_child, new_child, last_child; + + extern struct tms *t_init, *t_last, *t_new; + extern clock_t init_times, last_times, new_times; + + new_times = times(t_new); + + if(action == 0){ + fprintf(fp,"Elapsed time: %*6.2f\n", + (float)(new_times-last_times)/CLK_TCK); + } + + if(action == 1){ + /* compressed output - shows summary of user + sys for parent and child */ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + + + fprintf(fp,"CPU(P%7.1f%7.1f)(C%7.1f%7.1f)(T%7.1f%7.1f)(E%7.1f%7.1f)\n", + (float)(new_calling - last_calling)/CLK_TCK, + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_child - last_child)/CLK_TCK, + (float)(new_child - init_child)/CLK_TCK, + (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK, + (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK, + (float)(new_times-last_times)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK); + } + + + if(action == 2){ + /* full output */ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + + + fprintf(fp," %7s:%7s:%7s|%7s:%7s:%7s:%7s:%7s\n", + "user","sys","total","c user","c sys","ctotal","el/tcpu","cum"); + fprintf(fp,"Master :%7.1f:%7.1f:%7.1f|%7.1f:%7.1f:%7.1f|%7.1f:%7.1f\n", + (float)(t_new->tms_utime - t_last->tms_utime)/CLK_TCK, + (float)(t_new->tms_stime - t_last->tms_stime)/CLK_TCK, + (float)(new_calling - last_calling)/CLK_TCK, + (float)(t_new->tms_utime - t_init->tms_utime)/CLK_TCK, + (float)(t_new->tms_stime - t_init->tms_stime)/CLK_TCK, + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_times-last_times)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK); + + fprintf(fp,"Children:%7.1f:%7.1f:%7.1f|%7.1f:%7.1f:%7.1f|%7.1f:%7.1f\n", + (float)(t_new->tms_cutime - t_last->tms_cutime)/CLK_TCK, + (float)(t_new->tms_cstime - t_last->tms_cstime)/CLK_TCK, + (float)(new_child - last_child)/CLK_TCK, + (float)(t_new->tms_cutime - t_init->tms_cutime)/CLK_TCK, + (float)(t_new->tms_cstime - t_init->tms_cstime)/CLK_TCK, + (float)(new_child - init_child)/CLK_TCK, + (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK, + (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK); + } + if(action == 3){ + /* very compressed output - shows cumparent, cumchild + sum of the two and elapsed time summary*/ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + fprintf(fp,"Cum CPU Time: Parent: %*7.1f Child: %*7.1f Total: %*7.1f Elapsed: %7.1f\n", + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_child - init_child)/CLK_TCK, + (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK); + } + + copy_tms(t_last,t_new); + last_times = new_times; +} + + +char *GJ_get_times(int action) +{ + /* show the time(s) since init_times was called + if action = 0, print the time since the last call to GJ_init_times + or GJ_show_times. + if action = 1, print as in 0, but add the total time since the + first call to GJ_init_times. + + */ + + clock_t init_calling, new_calling, last_calling; + clock_t init_child, new_child, last_child; + + char *outbuf; + + extern struct tms *t_init, *t_last, *t_new; + extern clock_t init_times, last_times, new_times; + + new_times = times(t_new); + + outbuf = (char *) GJmalloc(sizeof(char) *200); + + if(action == 0){ + sprintf(outbuf,"%7.1f\0", + (float)(new_times-last_times)/CLK_TCK); + } +#ifdef KEEP_THIS + if(action == 1){ + /* compressed output - shows summary of user + sys for parent and child */ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + sprintf(outbuf,"%7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f\0", + (float)(new_calling - last_calling)/CLK_TCK, + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_child - last_child)/CLK_TCK, + (float)(new_child - init_child)/CLK_TCK, + (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK, + (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK, + (float)(new_times-last_times)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK); + } +#else + /* this is a version that ignores the child processes since these + appear not to be reported on the SGI */ + if(action == 1){ + /* compressed output - shows summary of user + sys for parent and child */ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + sprintf(outbuf,"%7.1f %7.1f %7.1f %7.1f\0", + (float)(new_calling - last_calling)/CLK_TCK, + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_times-last_times)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK); + } +#endif + + if(action == 2){ + /* full output */ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + + + sprintf(outbuf, + "%7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f %7.1f", + (float)(t_new->tms_utime - t_last->tms_utime)/CLK_TCK, + (float)(t_new->tms_stime - t_last->tms_stime)/CLK_TCK, + (float)(new_calling - last_calling)/CLK_TCK, + (float)(t_new->tms_utime - t_init->tms_utime)/CLK_TCK, + (float)(t_new->tms_stime - t_init->tms_stime)/CLK_TCK, + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_times-last_times)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK, + (float)(t_new->tms_cutime - t_last->tms_cutime)/CLK_TCK, + (float)(t_new->tms_cstime - t_last->tms_cstime)/CLK_TCK, + (float)(new_child - last_child)/CLK_TCK, + (float)(t_new->tms_cutime - t_init->tms_cutime)/CLK_TCK, + (float)(t_new->tms_cstime - t_init->tms_cstime)/CLK_TCK, + (float)(new_child - init_child)/CLK_TCK, + (float)(new_calling-last_calling+new_child-last_child)/CLK_TCK, + (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK); + } + + if(action == 3){ + /* very compressed output - shows cumparent, cumchild + sum of the two and elapsed time summary*/ + init_calling = t_init->tms_utime + t_init->tms_stime; + init_child = t_init->tms_cutime + t_init->tms_cstime; + + last_calling = t_last->tms_utime + t_last->tms_stime; + new_calling = t_new->tms_utime + t_new->tms_stime; + + last_child = t_last->tms_cutime + t_last->tms_cstime; + new_child = t_new->tms_cutime + t_new->tms_cstime; + + sprintf(outbuf,"%7.1f %7.1f %7.1f %7.1f\0", + (float)(new_calling - init_calling)/CLK_TCK, + (float)(new_child - init_child)/CLK_TCK, + (float)(new_calling-init_calling+new_child-init_child)/CLK_TCK, + (float)(new_times-init_times)/CLK_TCK); + } + + copy_tms(t_last,t_new); + last_times = new_times; + outbuf = (char *) GJrealloc(outbuf,sizeof(char) * (strlen(outbuf) + 1)); + return outbuf; +} + +void GJ_reset_times() +{ + extern struct tms *t_init, *t_new, *t_last; + + if(t_init != NULL){ + GJfree(t_init); + t_init = NULL; + } + if(t_new != NULL){ + GJfree(t_new); + t_new = NULL; + } + if(t_last != NULL){ + GJfree(t_last); + t_last = NULL; + } +} +#define DELETE +#ifndef DELETE +main(int argc,char **argv) +{ + int i,j; + float l; + GJ_init_times(); + /* fprintf(stderr,"(0)%s\n",GJ_get_times(0));*/ + /* fprintf(stderr,"(1)%s\n",GJ_get_times(1));*/ + /* fprintf(stderr,"(2)%s\n",GJ_get_times(2));*/ + fprintf(stderr,"(3)%s\n",GJ_get_times(3)); + + for(i=0;i<5000;++i){ + for(j=0;j<5000;++j){ + l = i*j; + } + } + /* fprintf(stderr,"(0)%s\n",GJ_get_times(0));*/ + /* fprintf(stderr,"(1)%s\n",GJ_get_times(1));*/ + /* fprintf(stderr,"(2)%s\n",GJ_get_times(2));*/ + fprintf(stderr,"(3)%s\n",GJ_get_times(3)); + for(i=0;i<10000;++i){ + for(j=0;j<5000;++j){ + l = i*j; + } + } + /* fprintf(stderr,"(0)%s\n",GJ_get_times(0));*/ + /* fprintf(stderr,"(1)%s\n",GJ_get_times(1));*/ + /* fprintf(stderr,"(2)%s\n",GJ_get_times(2));*/ + fprintf(stderr,"(3)%s\n",GJ_get_times(3)); + GJ_reset_times(); +} +#endif + + + + + diff --git a/sources/alscript/gjutil/gjtimes.h b/sources/alscript/gjutil/gjtimes.h new file mode 100644 index 0000000..9d44032 --- /dev/null +++ b/sources/alscript/gjutil/gjtimes.h @@ -0,0 +1,16 @@ +#ifndef GJ_TIMES_H +#define GJ_TIMES_H + +#include +#include +#include + +void GJ_init_times(void); +void GJ_show_times(FILE *fp,int action); +void GJ_reset_times(void); +char *GJ_get_times(int action); + +void copy_tms(struct tms *l,struct tms *r); + +#endif /*GJ_TIMES */ + diff --git a/sources/alscript/gjutil/gjutil.c b/sources/alscript/gjutil/gjutil.c new file mode 100644 index 0000000..e7f6408 --- /dev/null +++ b/sources/alscript/gjutil/gjutil.c @@ -0,0 +1,986 @@ + +/**************************************************************************** + +gjutil.c: Various utility routines - error checking malloc and +free, string functions etc... + +Copyright: Geoffrey J. Barton (1992, 1993, 1995, 1997) +email: geoff@ebi.ac.uk + +This software is made available for educational and non-commercial research +purposes. + +For commercial use, a commercial licence is required - contact the author +at the above address for details. + + +******************************************************************************/ +#include +#include +#include +#include +#include +#include +#include + +#include +#include + +/* define pointers for standard streams to allow redefinition to files */ + +FILE *std_err; +FILE *std_in; +FILE *std_out; + +/* clock externs */ +clock_t start_time, end_time,initial_time,final_time; + +void *GJmalloc(size_t size) +/* malloc with simple error check */ +/* G. J. Barton 1992 */ +{ + void *ptr; + ptr = (void *) malloc(size); + if(ptr == NULL){ + GJerror("malloc error"); + exit(0); + } + return ptr; +} + +void *GJrealloc(void *ptr,size_t size) +/* realloc with error check */ +/* G. J. Barton 1992 */ +{ + ptr = (void *) realloc(ptr,size); + if(ptr == NULL){ + GJerror("realloc error"); + exit(0); + } + return ptr; +} +void *GJmallocNQ(size_t size) +/* as for GJmalloc, but returns NULL on error*/ +/* G. J. Barton 1992 */ +{ + void *ptr; + ptr = (void *) malloc(size); + if(ptr == NULL){ + GJerror("malloc error"); + return NULL; + } + return ptr; +} + +void *GJreallocNQ(void *ptr,size_t size) +/* as for GJrealloc with error check but returns NULL on error*/ +/* G. J. Barton 1992 */ +{ + ptr = (void *) realloc(ptr,size); + if(ptr == NULL){ + GJerror("realloc error"); + return NULL; + } + return ptr; +} +void GJfree(void *ptr) +/* free with error check */ +/* G. J. Barton 1992 */ +{ + if(ptr == NULL){ + GJerror("Attempt to free NULL pointer"); + exit(0); + } + free(ptr); +} + +void GJerror(const char *prefix) +/* writes error message contained in prefix and contents of errno + to std_err. +*/ +/* G. J. Barton 1992 */ +{ + if(prefix != NULL){ + if(*prefix != '\0'){ + fprintf(std_err,"%s: ",prefix); + } + } + fprintf(std_err,"%s\n",strerror(errno)); +} + +/* +error: calls GJerror +*/ +void error(const char *str,int flag) +{ + GJerror(str); + if(flag)exit(0); +} + + +char *GJstoupper(const char *s) +/* return a copy of s in upper case */ +/* G. J. Barton 1992 */ +{ + char *temp; + int i; + temp = GJstrdup(s); + i=0; + while(temp[i] != '\0'){ + temp[i] = toupper(temp[i]); + ++i; + } + return temp; +} +char *GJstolower(const char *s) +/* return a copy of s in lower case */ +/* G. J. Barton 1992 */ +{ + char *temp; + int i; + temp = GJstrdup(s); + i=0; + while(temp[i] != '\0'){ + temp[i] = tolower(temp[i]); + ++i; + } + return temp; +} +char *GJstoup(char *s) +/* return s in upper case */ +/* G. J. Barton 1992 */ +{ + int i; + i=0; + while(s[i] != '\0'){ + s[i] = toupper(s[i]); + ++i; + } + return s; +} +char *GJstolo(char *s) +/* return s in lower case */ +/* G. J. Barton 1992 */ +{ + int i; + i=0; + while(s[i] != '\0'){ + s[i] = tolower(s[i]); + ++i; + } + return s; +} + +char *GJstrdup(const char *s) +/* returns a pointer to a copy of string s */ +/* G. J. Barton 1992 */ + +{ + char *temp; + temp = (char *) GJmalloc(sizeof(char) * (strlen(s)+1)); + temp = strcpy(temp,s); + return temp; +} + +char *GJstrrename(char *old,const char *new) +/* takes old which is a pointer to a string, then replaces the contents + of the string with new, reallocating to the correct size +*/ +{ + int nlen; + nlen = strlen(new); + old = (char *) GJrealloc(old,sizeof(char) * (nlen + 1)); + old = strcpy(old,new); + return old; +} + + + +FILE *GJfopen(const char *fname,const char *type,int action) +/* a file open function with error checking. The third argument +is set to 0 if we want a failed open to return, or 1 if we +want a failed open to exit the program. +*/ +/* G. J. Barton 1992 */ +/* modified July 1995 - error message only printed if action is 1 */ +{ + FILE *ret_val; + ret_val = fopen(fname,type); + if(ret_val == NULL){ + /* GJerror(strcat("Cannot Open File: ",fname));*/ + if(action == 1){ + GJerror(strcat("Cannot Open File: ",fname)); + exit(1); + } + } + return ret_val; +} + +int GJfclose(FILE *fp,int action) +/* a file close function with error checking. The second argument +is set to 0 if we want a failed close to return, or 1 if we +want a failed close to exit the program. +*/ +/* G. J. Barton 1992 */ +{ + int ret_val; + ret_val = fclose(fp); + if(ret_val != 0){ + if(action == 1){ + GJerror("Error closing File"); + exit(1); + } + } + return ret_val; +} + + +GJFILE *GJfilemake(const char *name,const char *type,int action) +/* If action = 1 then +Tries to open the file with the given name. If successful returns +a pointer to a struct file structure with the name and filehandle. If +the open fails, or action= 0 then returns a struct file structure +with the name and a NULL filehandle */ +/* G. J. Barton 1995 */ +{ + GJFILE *ret_val; + ret_val = (GJFILE *) GJmalloc(sizeof(GJFILE)); + ret_val->name = GJstrdup(name); + if(action == 1) { + ret_val->handle = GJfopen(ret_val->name,type,0); + }else if(action == 0){ + ret_val->handle = NULL; + } + return ret_val; +} + +GJFILE *GJfilerename(GJFILE *ret_val, const char *name) +/* When passed the fval structure - renames the name part of the +file structure to name, if the handle is non null it tries to close +the file, then sets the file handle to NULL. */ +/* G. J. Barton 1995 */ +{ + if(ret_val->name != NULL) { + GJfree(ret_val->name); + ret_val->name = GJstrdup(name); + } + if(ret_val->handle != NULL) { + GJfclose(ret_val->handle,0); + ret_val->handle = NULL; + } + return ret_val; +} + +GJFILE *GJfileclose(GJFILE *ret_val,int action) +/* Closes a file named in the struct file structure returns the struct + file structure */ + +/* G. J. Barton July 1995 */ +{ + STD_FILES; + + if(GJfclose(ret_val->handle,0) == 0){ + return ret_val; + }else { + if(action == 1){ + GJerror("Error closing File"); + fprintf(std_err,"%s\n",ret_val->name); + exit(-1); + } + } + return ret_val; +} + +GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action) +/* Opens a file named in the struct file structure */ + +/* G. J. Barton October 1995 */ +{ + STD_FILES; + + ret_val->handle = GJfopen(ret_val->name,type,0); + if(ret_val->handle == NULL){ + if(action == 1){ + GJerror("Error opening File"); + fprintf(std_err,"%s\n",ret_val->name); + exit(-1); + } + } + return ret_val; +} + +GJFILE *GJfileclean(GJFILE *ret_val,int action) +/* Closes the file then sets the file pointer to NULL, then + frees the filename string */ + +/* G. J. Barton July 1995 */ +{ + if(GJfclose(ret_val->handle,0) == 0) { + ret_val->handle = NULL; + GJfree(ret_val->name); + return ret_val; + }else { + if(action == 1){ + GJerror("Error closing File"); + fprintf(std_err,"%s\n",ret_val->name); + exit(-1); + } + } + return ret_val; +} + +void GJinitfile(void) +/* just set the standard streams */ +{ + std_err = stderr; + std_in = stdin; + std_out = stdout; +} + +char *GJfnonnull(char *string) +/* return pointer to first non null character in the string */ +/* this could cause problems if the string is not null terminated */ +{ + while(*string != '\0'){ + ++string; + } + return ++string; +} + +char *GJstrappend(char *string1, char *string2) +/* appends string2 to the end of string2. Any newline characters are removed +from string1, then the first character of string2 overwrites the null at the +end of string1. +string1 and string2 must have been allocated with malloc. +*/ +/* G. J. Barton July 1992 */ +{ + char *ret_val; + ret_val = GJremovechar(string1,'\n'); + ret_val = (char *) GJrealloc(ret_val, + sizeof(char) * (strlen(ret_val) + strlen(string2) + 1)); + ret_val = strcat(ret_val,string2); + return ret_val; +} + +char *GJremovechar(char *string,char c) +/* removes all instances of character c from string + returns a pointer to the reduced, null terminated string + 11/8/1996: couple of bugs found in this routine. + the length of the string returned was too short by 2 bytes. + This is a dodgy routine since string is freed. +*/ +/* G. J. Barton (July 1992) */ +{ + char *temp; + int j,i,nchar; + nchar = 0; + i=0; + while(string[i] != '\0'){ + if(string[i] == c){ + ++nchar; + } + ++i; + } + if(nchar == 0){ + return string; + }else{ + temp = (char *) GJmalloc(sizeof(char) * (strlen(string)-nchar + 1)); + j=0; + i=0; + while(string[i] != '\0'){ + if(string[i] != c){ + temp[j] = string[i]; + ++j; + } + ++i; + } + temp[j] = '\0'; + GJfree(string); + return temp; + } +} + +char *GJremovechar2(char *string,char c) +/* removes all instances of character c from string + returns a pointer to the reduced, null terminated string +*/ +/* G. J. Barton (July 1992) */ +{ + char *temp; + int i,k,len; + k=0; + len=strlen(string); + temp = (char *) GJmalloc(sizeof(char) * (len+1)); + for(i=0;i<(len+1);++i){ + if(string[i] != c){ + temp[k] = string[i]; + ++k; + } + } + for(i=0;i<(strlen(temp)+1);++i){ + string[i] = temp[i]; + } + GJfree(temp); + return string; +} + + +char *GJsubchar(char *string,char c2,char c1) +/* substitutes c1 for c2 in string +*/ +/* G. J. Barton (July 1992) */ +{ + int i; + + i=0; + while(string[i] != '\0'){ + if(string[i] == c1){ + string[i] = c2; + } + ++i; + } + return string; +} + +/* create a string and if fchar != NULL fill with characters */ +/* always set the len-1 character to '\0' */ + +char *GJstrcreate(size_t len,char *fchar) +{ + char *ret_val; + ret_val = (char *) GJmalloc(sizeof(char) * len); + --len; + ret_val[len] = '\0'; + if(fchar != NULL){ + while(len > -1){ + ret_val[len] = *fchar; + --len; + } + } + return ret_val; +} + +/* searches for string s2 in string s1 and returns pointer to first instance +of s2 in s1 or NULL if no instance found. s1 and s2 must be null terminated +*/ +char *GJstrlocate(char *s1, char *s2) +{ + int i=0; + int j=0; + int k; + if(strlen(s1) == 0 || strlen(s2) == 0) return NULL; + while(i < strlen(s1)){ + j=0; + k=i; + while(j < strlen(s2) && s1[k] == s2[j]){ + ++k; + ++j; + } + if(j == strlen(s2)) return &s1[i]; + ++i; + } + return NULL; +} +#include +#include + + +/* GJstrtok() + +This version of strtok places the work pointer at the location of the first +character in the next token, rather than just after the last character of the +current token. This is useful for extracting quoted strings +*/ + +char *GJstrtok(char *input_string,const char *token_list) +{ + static char *work; + char *return_ptr; + + if(input_string != NULL){ + /* first call */ + work = input_string; + } + + /* search for next non-token character */ + while(strchr(token_list,*work)!=NULL){ + ++work; + } + + if(*work == '\0'){ + /* if we've reached the end of string, then return NULL */ + return NULL; + }else{ + return_ptr = (char *) work; + while(strchr(token_list,*work) == NULL){ + if(*work == '\0'){ + /* end of the string */ + return return_ptr; + }else{ + ++work; + } + } + *work = '\0'; + ++work; + /* now increment work until we find the next non-delimiter character */ + while(strchr(token_list,*work) != NULL){ + if(*work == '\0'){ + break; + }else{ + ++work; + } + } + return return_ptr; + } +} +/************************************************************************** +return a pointer to space for a rectangular unsigned character array +Version 2.0 ANSI and uses GJmallocNQ +--------------------------------------------------------------------------*/ + +unsigned char **uchararr(int i,int j) +{ + unsigned char **temp; + int k, rowsiz; + + temp = (unsigned char **) GJmallocNQ(sizeof(unsigned char *) * i); + if(temp == NULL) return NULL; + + rowsiz = sizeof(unsigned char) * j; + + for (k = 0; k < i; ++k){ + temp[k] = (unsigned char *) GJmallocNQ(rowsiz); + if(temp[k] == NULL) return NULL; + } + return temp; +} + +/************************************************************************** +free up space pointed to by rectangular unsigned character array +-------------------------------------------------------------------------*/ +void ucharfree(unsigned char **array,int i) + +{ + int k; + + for (k = 0; k < i; ++k){ + GJfree((char *) array[k]); + } + GJfree((char *) array); + +} + +/************************************************************************** +return a pointer to space for a rectangular signed character array +Version 2.0 ANSI +--------------------------------------------------------------------------*/ +signed char **chararr(int i,int j) + +{ + signed char **temp; + int k, rowsiz; + + temp = (signed char **) GJmallocNQ(sizeof(char *) * i); + + if(temp == NULL) return NULL; + + rowsiz = sizeof(char) * j; + + for (k = 0; k < i; ++k){ + temp[k] = (signed char *) GJmallocNQ(rowsiz); + if(temp[k] == NULL) return NULL; + } + return temp; +} + + +/* mcheck - check a call to malloc - if the call has failed, print the +error message and exit the program */ +/* ANSI Version - also uses GJerror routine and ptr is declared void*/ + +void mcheck(void *ptr,char *msg) + +{ + if(ptr == NULL){ + GJerror("malloc/realloc error"); + exit(0); + } +} + +/* set a string to blanks and add terminating nul */ +char *GJstrblank(char *string,int len) + +{ + --len; + string[len] = '\0'; + --len; + while(len > -1){ + string[len] = ' '; + --len; + } + return string; +} + +/* Initialise an unsigned char array */ +void GJUCinit(unsigned char **array,int i,int j,unsigned char val) +{ + int k,l; + + for(k=0;k0){ + token[i] = '\0'; + return token; + } + } +/* GJerror("End of File Encountered");*/ + GJfree(token); + return NULL; +} + +struct tokens *GJgettokens(const char *delims, char *buff) +/* This splits a buffer into tokens at each position defined by delims.*/ +/* The structure returned contains the number of tokens and the */ +/* tokens themselves as a char ** array */ +{ + char *token; + struct tokens *tok; + + token = strtok(buff,delims); + if(token == NULL) return NULL; + + tok = (struct tokens *) GJmalloc(sizeof(struct tokens)); + tok->ntok = 0; + tok->tok = (char **) GJmalloc(sizeof(char *)); + tok->tok[0] = GJstrdup(token); + ++tok->ntok; + while((token = strtok(NULL,delims)) != NULL) { + tok->tok = (char **) GJrealloc(tok->tok,sizeof(char *) * (tok->ntok+1)); + tok->tok[tok->ntok] = GJstrdup(token); + ++tok->ntok; + } + + return tok; +} + +void GJfreetokens(struct tokens *tok) +/* frees a tokens structure */ + +{ + int i; + for(i=0;intok;++i) { + GJfree(tok->tok[i]); + } + GJfree(tok->tok); + GJfree(tok); + tok = NULL; /* add this to avoid odd pointer 27/6/1997*/ +} + +char * GJtoktostr(struct tokens *tok,char delim,int s, int e) + +/* + returns a string with the tokens between s and e inclusive written to + it separated by delim + the tok structure is unmodified. +*/ + +{ + int n, i, j,k; + char *ret_val; + + n = 0; + + + if(s < 0 || s >= tok->ntok) s = 0; + if(e < 0 || e >= tok->ntok) e = tok->ntok - 1; + + for(i=s;i<=e;++i){ + n += strlen(tok->tok[i]); + ++n; + } + + ret_val = (char *) GJmalloc(sizeof(char) * n); + j = 0; + for(i=s;i<=e;++i){ + for(k=0;ktok[i]);++k){ + ret_val[j] = tok->tok[i][k]; + ++j; + } + ret_val[j++] = delim; + } + ret_val[n-1] = '\0'; + return ret_val; +} + +/* +void GJ_start_clock() +{ + extern clock_t start_time, end_time,initial_time,final_time; + start_time = clock(); + initial_time = start_time; +} + +void GJ_stop_clock(FILE *fp) +{ + extern clock_t start_time, end_time,initial_time,final_time; + end_time = clock(); + fprintf(fp,"CPU time: %f seconds\n", + ((float) (end_time - start_time))/CLOCKS_PER_SEC); +} +*/ + + +void GJ_start_clock(void) +{ + GJ_init_times(); +} + +void GJ_stop_clock(FILE *fp) +{ + GJ_show_times(fp,3); + GJ_reset_times(); +} + + +void GJindexx(int *arrin,int n,int *indx) + /* indexed heap sort - adapted from the NR routine indexx. + inarr is an integer array to sort, + indx is the returned index array + */ +{ + int l,j,ir,indxt,i; + float q; + + for (j=1;j<=n;j++) indx[j]=j; + l=(n >> 1) + 1; + ir=n; + for (;;) { + if (l > 1) + q=arrin[(indxt=indx[--l])]; + else { + q=arrin[(indxt=indx[ir])]; + indx[ir]=indx[1]; + if (--ir == 1) { + indx[1]=indxt; + return; + } + } + i=l; + j=l << 1; + while (j <= ir) { + if (j < ir && arrin[indx[j]] < arrin[indx[j+1]]) j++; + if (q < arrin[indx[j]]) { + indx[i]=indx[j]; + j += (i=j); + } + else j=ir+1; + } + indx[i]=indxt; + } +} + + + +void GJpline(FILE *fp,char c,int n) +/* print n copies of c to fp terminated by newline */ +{ + int i; + for(i=0;i n){ + for(i=0;i imax) imax = ivec[i]; + if(ivec[i] < imin) imin = ivec[i]; + } + + ret_val = (IRANGE *) GJmalloc(sizeof(IRANGE)); + ret_val->min = imin; + ret_val->max = imax; + + return ret_val; +} + +/* + +$Id: gjutil.c,v 1.1.1.1 1999/07/09 13:34:10 geoff Exp $ + +$Log: gjutil.c,v $ +Revision 1.1.1.1 1999/07/09 13:34:10 geoff +Import alscript 2_07 with the cygwin executables. + +Revision 1.9 1999/07/09 13:34:10 geoff +modified these as a test + +Revision 1.8 1998/08/11 15:38:50 geoff +Modified the copyright notice to reflect the new +ownership of this software. + +Revision 1.7 1997/06/29 00:43:57 gjb +Changes to add sysinfo calls and test of license reading routines + +Revision 1.6 1997/06/27 16:42:41 gjb +Add trlic.c test.lic and clean up gjutil.c + +Revision 1.5 1997/06/27 07:17:31 gjb +Added rlic.c linfo.h and +changes to gjutil.c to give better support for +token manipulation + +Revision 1.4 1997/05/12 11:10:53 gjb +Re-added gjutil.c and gjutil.h to repository +after deleting them + +Revision 1.2 1997/05/12 10:47:52 gjb +Modified CVS header and log position + +*/ diff --git a/sources/alscript/gjutil/gjutil.h b/sources/alscript/gjutil/gjutil.h new file mode 100644 index 0000000..f22d241 --- /dev/null +++ b/sources/alscript/gjutil/gjutil.h @@ -0,0 +1,109 @@ +#ifndef GJ_UTIL_H +#define GJ_UTIL_H + +/* +$Id: gjutil.h,v 1.1.1.1 1997/06/27 07:17:32 geoff Exp $ +*/ + +#include + + +typedef int GJ_LIM; /* type for limits - e.g. max buffer length */ +typedef int GJ_PEN; /* type for gap penalties */ +typedef int GJ_FLG; /* Flag type - usually just 1 or 0 */ +typedef float GJ_FLOAT; /* Single precision floating point type */ +typedef double GJ_DBL; /* a double precision floating point type */ +typedef int GJ_S_COUNT; /* small counter */ +typedef long GJ_L_COUNT; /* long counter */ + +typedef struct { /* structure to hold a filename and */ + char *name; /* associated handle */ + FILE *handle; +} GJFILE; + +struct tokens { /* structure to hold tokens parsed from */ + int ntok; /* string with strtok */ + char **tok; +}; + +typedef struct { + int min; + int max; +} IRANGE; + +#define STD_FILES extern FILE *std_in,*std_out,*std_err + +/* utility.h function definitions */ + +void *GJmalloc(size_t); +void *GJrealloc(void *,size_t); +void *GJmallocNQ(size_t); +void *GJreallocNQ(void *,size_t); +void GJfree(void *); +void GJerror(const char *); +char *GJstrdup(const char *); +char *GJstoupper(const char *); +char *GJstolower(const char *); +char *GJstoup(char *); +char *GJstolo(char *); + +FILE *GJfopen(const char *, const char *,int); +int GJfclose(FILE *,int); +GJFILE *GJfilemake(const char *name,const char *type,int action); +GJFILE *GJfilerename(GJFILE *ret_val, const char *name); +GJFILE *GJfileclose(GJFILE *ret_val,int action); +GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action); +GJFILE *GJfileclean(GJFILE *fval,int action); +void GJinitfile(void); + +char *GJfnonnull(char *); +char *GJstrappend(char *,char *); +char *GJremovechar(char *,char); +char *GJremovechar2(char *string,char c); +char *GJstrcreate(size_t, char *); +char *GJstrlocate(char *,char *); +char *GJsubchar(char *,char,char); +char *GJstrtok(char *,const char *); +void error(const char *, int); +unsigned char **uchararr(int,int); +void ucharfree(unsigned char **array,int i); +signed char **chararr(int,int); +void GJCinit(signed char **,int ,int ,char ); +void mcheck(void *, char *); +char *GJstrblank(char *, int); +void GJUCinit(unsigned char **,int ,int ,unsigned char ); +char *GJcat(int N,...); +struct tokens *GJgettokens(const char *delims, char *buff); +void GJfreetokens(struct tokens *tok); +char * GJtoktostr(struct tokens *tok, char delim, int s, int e); +void GJ_start_clock(void); +void GJ_stop_clock(FILE *fp); +char *GJstrrename(char *old,const char *new); +void GJindexx(int *arrin,int n,int *indx); + +void GJpline(FILE *fp,char c,int n); +char *GJlocaltime(void); +void GJpstring(FILE *fp,char *s,int n); + +IRANGE *irange(int *ivec, int n); + +/* +$Log: gjutil.h,v $ +Revision 1.1.1.1 1997/06/27 07:17:32 geoff +Import alscript 2_07 with the cygwin executables. + +Revision 1.5 1997/06/27 07:17:32 gjb +Added rlic.c linfo.h and +changes to gjutil.c to give better support for +token manipulation + +Revision 1.4 1997/05/12 11:10:54 gjb +Re-added gjutil.c and gjutil.h to repository +after deleting them + +Revision 1.2 1997/05/12 10:47:52 gjb +Modified CVS header and log position + +*/ +#endif /* GJ_UTIL_H */ + diff --git a/sources/alscript/src/.pure b/sources/alscript/src/.pure new file mode 100755 index 0000000..e69de29 diff --git a/sources/alscript/src/agetbloc.c b/sources/alscript/src/agetbloc.c new file mode 100644 index 0000000..b3bea0e --- /dev/null +++ b/sources/alscript/src/agetbloc.c @@ -0,0 +1,135 @@ +/**************************************************************************** + + AGETBLOC - a routine to read an AMPS block file + + Copyright: Geoffrey J. Barton (1992,1997) + + email: geoff@ebi.ac.uk + + Please see README for conditions of use. + +**************************************************************************** + +History: + +15th November 1992 - ANSI C version - also uses GJ... routines. +This version adapted with error messages for alscript. + +11th June 1992. +Agetbloc: like getbloc, but does not require that every character read into +the seqs structure is an alphabetic character. Also does not contain the +option to convert the "sequences" read in into integer format + +getbloc: Read an AMPS style block file into the seqs array +the nbloc aligned sequences are stored in positions 1-nbloc. + +This is a straight-ish translation of the fortran routine fbloc.f, hence +the non-C like goto's... + +Sequence lengths are actual length +1 + 1. This allows position 0 to +be reserved for future use, and preserves the '\0' for output. + +18/Feb/1993:Fix (i) to (i+1) in realloc. Spotted by RBR. + + $Id: agetbloc.c,v 1.2 1998/09/17 16:54:58 geoff Exp $ + $Log: agetbloc.c,v $ + Revision 1.2 1998/09/17 16:54:58 geoff + Check consistency with archive + + +*/ + +#include +#include +#include +#include +#include "array.h" +#include "gjutil.h" + + +int Agetbloc(FILE *bfile,struct seqdat *bloc,int *nbloc) + +{ + int i,llen; + extern int MAXnseq, MAXslen, MAXilen, MAXtlen, MAXnbloc; + char *buff; + extern FILE *std_in,*std_out,*std_err; + + char *idstart, *idend, *bstart, sident = 0; + int idlen,totseq = 0,k,j; + + buff = (char *) GJmalloc(sizeof(char) * MAXtlen); + +l1: + buff = fgets(buff,MAXtlen,bfile); + if(buff == NULL){ + fprintf(std_err,"Premature end of BLOCK FILE\n"); + return 0; + } + if((idstart = strchr(buff,'>')) != NULL){ + if(++totseq == MAXnbloc){ + fprintf(std_err, + "Max Number of block file sequences exceeded: %d\n", + totseq); + fprintf(std_err,"Use MAX_NSEQ command to increase value"); + return 0; + } + sident = 1; + idend = strchr(idstart,' '); + if(idend == NULL){ + idend = strchr(idstart,'\0'); + } + if(idend == NULL){ + fprintf(std_err,"Error reading identifier:%s\n",idstart); + error("Exiting",1); + } + idlen = (idend - idstart) + 1; + bloc[totseq].id = (char *) malloc(sizeof(char) * idlen); + bloc[totseq].id = GJstrblank(bloc[totseq].id,idlen); + strncpy(bloc[totseq].id,idstart+1,idlen-1); /* don't copy the ">" symbol */ + bloc[totseq].ilen = idlen-1; + bloc[totseq].id[idlen-1] = '\0'; + + bloc[totseq].tlen = strlen(idend)+1; + bloc[totseq].title = (char *) GJmalloc(sizeof(char) * bloc[totseq].tlen); + bloc[totseq].title = GJstrblank(bloc[totseq].title,bloc[totseq].tlen); + strcpy(bloc[totseq].title,idend); + + bloc[totseq].seq = (char *) GJmalloc(sizeof(char) * MAXslen); + bloc[totseq].seq[0] = ' '; + goto l1; + } else if(sident){ + if((idstart = strchr(buff,'*')) != NULL){ + i = 0; + while((buff = fgets(buff,MAXtlen,bfile)) != NULL){ + if(*idstart == '*'){ +/* fprintf(stdout,"Blocfile read: Length: %d\n",i);*/ + ++i; + for(k=1;k +#include +#include +#include "array.h" +#include "gjutil.h" +#include "version.h" + +/**************************************************************************** + + ALPS - Main routines for ALSCRIPT alignment formatting tool + + Copyright: Geoffrey J. Barton (1992,1993,1997) + + email: geoff@ebi.ac.uk + +All use of this program must cite: Barton, G. J. (1993), ALSCRIPT: A Tool to +Format Multiple Sequence Alignments, Protein Engineering, Volume 6, No. 1, +pp. 37-40. + + Please see README file for details of limitations of use. + + $Id: alps.c,v 1.3 1998/09/17 17:34:26 geoff Exp $ + $Log: alps.c,v $ + Revision 1.3 1998/09/17 17:34:26 geoff + Add the new README file and the LICENSE and RELEASE_NOTES + + Revision 1.2 1998/09/17 16:54:58 geoff + Check consistency with archive + + +**************************************************************************** + + Main routine for ALSCRIPT - formally called ALPS + + alps: 11th June 1992. + Author: G. J. Barton + + This file contains a set of subroutines that provide for flexible +formatting of multiple sequence alignments in PostScript at +publication quality. + +11-18th June 1992: + +1. Modified getbloc.c to Agetbloc.c. Agetbloc reads all characters +(ie not just legal amino acid codes) from an AMPS blocfile format +multiple alignment file. + +2. Wrote basic page scaling features to allow arbitrary page +dimensions to be defined and to allow all x,y locations to be defined +in terms of characters of the chosen pointsize. The number of +residues per line and hence number of pages is determined by the +pointsize. The page is divided into four areas. (a) The main region +for plotting out the amino acid sequences. (b) A region to the left of +a for plotting sequence identifier codes - the width of this region is +set by variable Cidwidth. (c) A region above region a, and (d) a +region below a. Regions c and d are initially set to zero size. +Routine ALReserveSpace() allows a selected number of lines to be +reserved above or below for plotting. + +3. Routines ALOutID and ALOutSeq written and multi-page plotting of +the sequences sorted out. Still limited to one page in the vertical +direction - toyed with the idea of writing single page PostScript and +using PostScript to modify clipping boundaries like in the Poster demo +program in the CookBook. + +4. Experiment with simple boxing facility - ALDrawSimpleBox(). This +routine is incompatible with multi-page output since page breaks are +unpredictable. + +5. Develop sophisticated (!) boxing facility - defines boxes in terms +of TOP BOTTOM LEFT and RIGHT lines that may be defined around each +character routine ALLineRes() implements this feature. The details of +TOP etc for each character are stored in the unsigned char array +"lines". This uses the rightmost four bits to encode LEFT RIGHT etc. +Thus a line to the LEFT line is coded by decimal 1 (0001 binary), a +full box by decimal 15 (1111 binary). ALBoxRes and ALBoxRegion +written to allow boxing of arbitrary regions. + +6. The y coordinate sent to the low level ALLineRes routine +refers directly to the index to the lines array. If you want to work in +residue coordinate space, you need to flip the y coordinate before +writing to the lines array (also the fill array). Routines ALSurroundChars +and ALFillChars do this directly. Routines ALBoxRegion and ALFilRegion +make use of the Yindx array which does the flip in a much cleaner and more +elegant way. If I'd thought of it at the +time, I would have used this in ALSurroundChars and ALFillChars as well! + +7. Now add capability to split alignments vertically as well as horizontally. +There is now no limit on pointsize or number/length of sequences +(in principle!). Next move on the page layout front will be to allow +multiple blocks on a single page - this would permit alignments of a +few sequences, or small fonts to make better use of paper! + +8. We now have most of the subroutines working OK. So time to tidy up the +input. Add simple command parser to main programme - this is in two steps, +step 1 reads the basic page layout commands, step 2 reads the boxing/shading +font changing commands - Oh yes, added the font changing option as well. + +Current commands as follows: +Commands are delimited by space, comma, or tab. Newline signals the end of a +command. Unless otherwise stated, units are in characters of the default pointsize. +For a full list see the file "alscript.doc". + +step 1 commands: + +BLOCK_FILE %The name of the alignment file to format +OUTPUT_FILE %must have this near the beginning +LANDSCAPE %Longest side horizontal +PORTRAIT % vertical +POINTSIZE %Pointsize for page calculations and character spacing/default size. +IDENT_WIDTH %Width allocated to identifiers. +NUMBER_SEQS 1 || 0 %1=number identifiers, 0= no numbers (default). +DEFINE_FONT %define a font to be referred to as font number. +e.g. + DEFINE_FONT 0 Helvetica 10 %font 0 is Helvetica at 10 point + DEFINE_FONT 4 Times-Roman DEFAULT %font 4 is Times-Roman at the default pointsize. +SETUP %signals end of step 1 commands. + +step 2 commands: + +RESERVE_SPACE_TOP %make space at the top of the alignment +RESERVE_SPACE_BOTTOM % bottom DOES NOT WORK. +SURROUND_CHARS + %surround (box) the characters chars within the ranges specified +e.g. + SURROUND_CHARS PG 1 7 20 12 %surround P and G characters from residues 1 to 20 of sequences 7 to 12. + SURROUND_CHARS DEKHR ALL %surround D and E and K and R characters for the whole alignment. + +SHADE_CHARS + %similar syntax to SURROUND_CHARS with the addition of a grey value + % 0 = black, 1 = white. Note that shading is done before outputing the + % alignment. +e.g. + SHADE_CHARS PG 1 7 20 12 0.5 % shade P and G in the range shown with a grey value of 0.5 + SHADE_CHARS ILV ALL 0 % shade all I and L and V in black. + +FONT_CHARS + %same syntax as SHADE_CHARS, only give the font number in place of grey. + %font numbers must first be defined with the DEFINE_FONT command above. +FONT_RESIDUE sx sy font %allows setting of font for one residue (use with TEXT command) + +BOX_REGION + %draw a rectangular box around the characters within the defined range. +SHADE_REGION + %shade the region by the chosed grey value. + +9. Add option to place arbitrary text string at a character location. +e.g + TEXT 1 7 "SIGNAL PEPTIDE" %puts this text at sequence position 1 7. + %to change fonts use the FONT_RESIDUE or FONT_REGION commands. +10. Add option to change fonts of identifiers. +ID_FONT +e.g. + ID_FONT 3 1 %sets font to 1 for sequence identifier 3 + ID_FONT ALL 7 %uses font 7 for all identifiers + +19th June 1992: +Still fiddling - must get back to some real work soon. +Split alps away from the scanps program. Now have a separate main() for the routine. + +Add options to redefine all defaults: e.g. MAXslen (maximum sequence +length etc.) Add arbitrary line drawing options: eg. LINE TOP 20 15 50 +draws a line along the top of the characters from residue 20 to 50 or +sequence 15. Think about adding the option to change line thickness at +different parts of the plot. Decide not to implement that option right +now. Add option to include dummy sequences (ADD_SEQ) + +Check the programme compiles on the 386 PC. It does! +Write documentation and brief report of the programme. + +22nd June 1992: Get back to some real work! + +19th October: Add SILENT_MODE Command and carriage returns to all .PS print statements. +21st October: Add VERTICAL_SPACING command and ability to output multiple blocks per page. +15th November: Add pipe options to alscript.c Tidy up file handling. + +6th December: Add Colour options. +18th Jan 1993: Tidy up citations: modify Inverse chars to allow reversion. +1 March 1993: Fix bug in colour option. Pass use_colour to ALDrawLines routine to + specify black for line colour. Previously, this was drawn in white + if coloured shading had been specified on the same characters. +2 March 1993: Fix bug in colour option to output identifiers in black, not white! +9 March 1993: Add NO_NUMBERS option to avoid printing residue numbers. +24 March 1993: Add mask features. + +11 March 1994: At PERI - Add special text features - preliminary version only. + This permits strands and helices to be plotted as cylinders and arrows. + Special text features also permit triangles and circles etc etc. through + use of text command. + +23 May 1995: Numerous changes and additions over the last year - these +include the option to colour backgrounds differently, ommission of +idents on second and subsequent lines, helix, strand and other special +characters, relative numbering, error checking of ranges on input, +bounding box, screening, conservation colouring... + + +*/ + +#define TOKENS "\" ,\n\t" /* standard command token delimiters */ +#define POINT 72 /* Work in points throughout */ +#define C_ID_WIDTH 10 /* Default width for writing id's (in characters) */ +#define DEF_POINTSIZE 10.0 +#define X_OFFSET 40 /* offset for bottom lh corner of page (points) */ +#define Y_OFFSET 30 +#define DEF_YPOS 0 /* default first printinf position (character units) */ +#define LINE_WIDTH_FACTOR 0.05 /* Multiply pointsize by this to get linewidth */ +#define MAX_ADD_SEQ 10 /* Maximum number of ADD_SEQ commands allowed */ + +/*#define MAX_S 10.5 max and min page side in inches */ +/*#define MIN_S 6.5 */ +/*#define MAXSIDE (MAX_S * POINT) Default Max and min dimensions of paper */ +/*#define MINSIDE (MIN_S * POINT) */ + +#define SCREENSIZE 120 /* define default size for screen */ + + +int width = 0; /* max width and height of drawing area */ +int height = 0; +char *tokens = TOKENS; + +float MAXside = 10.5 * POINT; /* values to hold Max and min page side in points */ +float MINside = 6.5 * POINT; + +int xoff = X_OFFSET; +int yoff = Y_OFFSET; + +int Cidwidth = C_ID_WIDTH; /* width of id region in characters (left of sequence block)*/ +int Cseqwidth; /* width of sequence region in characters */ +int Cwidth; /* width/height of page in characters */ +int Cheight; +int Xspace; /* spacing between characters in X */ +int Yspace; /* spacing between characters in Y */ +float XspaceFactor = 0.2; /* extra bit to add to horizontal character spacing */ +float YspaceFactor = 0.0; /* extra bit to add to vertical character spacing */ +float XshiftFactor = 0.12; /* *pointsize gives the shift for vertical lines */ +float YshiftFactor = 0.12; /* horizontal lines */ + +int CseqTop = 0; +int CseqVoffset = 0; /* vertical offset for sequence block (characters)*/ +int CtopSpace =0; /* extra space above sequence block (characters) */ +int CbottomSpace = 0; /* " " below " " " */ +int Csheet = 1; /* number of vertical splits in sequence block */ + +int TVspace = 0; /* total vertical space reserved (characters)*/ + +float Xshift = 0; /* offsets for drawing box lines */ +float Yshift = 0; +float LineWidthFactor = LINE_WIDTH_FACTOR; + +float pointsize = DEF_POINTSIZE; +int npage; + +int Vspacing = 0; /* vertical spacing in characters between blocks on a page */ + + +enum Orientations{ + LANDSCAPE, + PORTRAIT +}; + +enum Spaces{ + TOP, + BOTTOM +}; + +struct addseq { + int pos; + int num; +}; + +/* bit masks for the lines array */ +unsigned char LINE_LEFT = 1; /* mask for line at left of char */ +unsigned char LINE_RIGHT= 2; /* right */ +unsigned char LINE_TOP = 4; /* top */ +unsigned char LINE_BOTTOM=8; /* bottom */ + +void ALOutId(struct seqdat *,int,unsigned char *colour, + unsigned char *,int,int,int,int, FILE *); +void ALOutSeq(struct seqdat *,unsigned char **, + char ***, + unsigned char **,int ,unsigned char **, + unsigned char **text_colour, + unsigned char background_colour, + int ,int ,int ,int ,FILE *); +void PSPutText(int,int,char *,FILE *); +void PSPutChar(int,int,char,FILE *); +void PSShowPage(FILE *); +void ALSetFont(char *,int,FILE *); +void ALCheckSinglePage(int ,int ,int ); +void ALSetPageLimits(void); +void PSSetOrientation(int, FILE *); +void PSPreamble(FILE *,int, int*, int screensize); +void PSLandscape(FILE *); +void PSPortrait(FILE *); +void PSSetupPage(int,int,FILE *); +void ALReserveSpace(int, int); +void ALReportInfo(int, FILE *); +char **ALCreateNumbers(int,int,int,int); +char *ALCreateTicks(int,int,int); +void ALOutTicks(char *,int,int,int,int,FILE *); +void ALOutNumbers(char **,int,int,int,int,FILE *); +void PSDrawBox(float,float,float,float,float,FILE *); +void ALSimpleBox(int,int,int,int,float,FILE *); +void PSline(float,float,float,float,FILE *); +void ALLineChar(unsigned char **,int,int,unsigned char); +void ALLineRes(unsigned char **,int,int,unsigned char); +void ALDrawLines(unsigned char **,int, + unsigned char **left_line_colour, + unsigned char **right_line_colour, + unsigned char **top_line_colour, + unsigned char **bottom_line_colour, + int,int,int,int,int,int,FILE *); +void ALBoxRes(unsigned char **,int,int); +void ALDrawFills(signed char **,int ,unsigned char **, + int,int ,int,int ,int ,int, FILE *); +void ALFilRes(signed char **,int,int,float); +void ALBoxRegion(unsigned char **,int,int,int,int,int *); +void ALFilRegion(signed char **,int ,int,int,int , int *,float); +void ALSurroundChars(struct seqdat *,unsigned char **,int,int,int,int,int,char *); +void ALShadeChars(struct seqdat *,signed char **, + int ,int,int,int,int,char *,float); +void ALDefineFont(int,char *,float,FILE *); +void echo(char *); +void ALGetFourInt(int *,int *,int *,int *); +void ALGetThreeInt(int *,int *,int *); +void ALGetTwoInt(int *,int *); +void ALFontChars(struct seqdat *,unsigned char **, + int,int,int,int,char *,unsigned char); +void ALFontRegion(unsigned char **,int,int,int,int,unsigned char); +void PSSetFont(unsigned char,FILE *); +void ALDoLine(unsigned char **,int,int,int,int,int *); +void ALSubChars(struct seqdat *,int,int,int,int,char,char); +char ALChekSpace(const char *); +void PSSetGrey(float,FILE *); +void ALInverseChars(struct seqdat *,unsigned char **,int,int,int,int,char *); +int Agetbloc(FILE *,struct seqdat *,int *); +void ALDefineColour(int,char *coltype, float,float,float,FILE *); +void ALSColChars(struct seqdat *,unsigned char **, + int,int,int,int,int,char *,unsigned char); +void ALColourChars(struct seqdat *,unsigned char **, + int ,int,int,int,char *,unsigned char); +void ALColRes(unsigned char **,int ,int , int ); +void PSSetColour(unsigned char,FILE *); +void ALColRegion(unsigned char **,int,int,int,int,int *,unsigned char); +void ALColRRegion(unsigned char **,int,int,int,int,unsigned char); +void ckdd(struct seqdat *, int); +int Ifound(struct seqdat *,int,int,char *,int); +void ALSColMask(unsigned char **,unsigned char **, + int, int, int, int,int, unsigned char); +void ALInverseMask(unsigned char **,unsigned char **,int, int, int, int); +void ALFontMask(unsigned char **,unsigned char **,int, int, int, int,unsigned char); +void ALShadeMask(unsigned char **,signed char **,int,int,int,int,int *, float); +void ALGetAllRange(int *, int *, int *, int *,int ,int ); +void ALid_mask(unsigned char **,struct seqdat *,int, int, int, int, int, char *, char *); +void ALfre_mask(unsigned char **mask,struct seqdat *,int, int, int, int, char *, char *); +int Ifound(struct seqdat *,int,int,char *,int); +void ALagree_mask(unsigned char **,struct seqdat *,int,int,int,int,int); +void ALnot_mask(unsigned char **,int, int, int, int); +void ALsub_mask(unsigned char **,struct seqdat *,int, int, int, int, char); +void ALMask(unsigned char **,int, int, int, int); +void ALConsMask(unsigned char **mask, +int sx,int sy,int ex,int ey,int *consval,int conscut); +void ALColourMask(unsigned char **,unsigned char **,int,int,int,int,unsigned char); +void ALSurroundMask(unsigned char **,struct seqdat *,unsigned char **,int,int,int,int,int); +int save_pir(struct seqdat *,int,FILE *); +void ALDrawHorizLine( + float Xpos, /* bottom left hand corner of character posn */ + float Ypos, /* */ + float Xspace, /* horizontal width of character position */ + float Yspace, /* vertical height of character position */ + float width_fac,FILE *outf); /* proportion of vertical height that the line will fill */ +void ALDrawRightArrow(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawLeftArrow(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawUpArrow(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawDownArrow(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawCircle(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawPseudoEllipse(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac, + FILE *outf); +void ALDrawLeftSemiEnd(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawRightSemiEnd(float Xpos,float Ypos, float Xspace, + float Yspace, float width_fac,FILE *outf); +void ALDrawRightHalfHorizLine(float Xpos,float Ypos,float Xspace, + float Yspace,float width_fac,FILE *outf); +void ALDrawLeftHalfHorizLine(float Xpos,float Ypos,float Xspace,float + Yspace,float width_fac,FILE *outf); +void PSmoveto(float x,float y,FILE *outf); +void PSlineto(float x,float y,FILE *outf); +void PSPutSpecialText(float Xpos,float Ypos,float Xspace, + float Yspace,char *text,unsigned char current_col, + unsigned char background_colour, + float YspaceFactor,FILE *outf); +void PSstroke(FILE *outf); +void PSnewpath(FILE *outf); +void ALColourBackground(FILE *outf,int colour,int *background_region); +void ALCheckLegalRange(int sx,int sy, int ex, int ey, int xmin, int ymin, int xmax, int ymax); +void ALMakeCoilText(int sx, int sy, int ex, char ***texts); +void ALMakeStrandText(int sx, int sy, int ex, char ***texts); +void ALMakeHelixText(int sx, int sy, int ex, char ***texts); +int ALrel(char *pos,int *relnum); +int GJindex(char *str,char c); +float mzcons(unsigned char *pos,int n); +int *ALCalCons(struct seqdat *bloc, + int sx,int sy,int ex,int ey,int *ret_val,int len); +int save_msf(struct seqdat *bloc,int nseq,FILE *msff); + +int alps(char *command_file,int silent, int pipe, int singlepage) +{ + FILE *cf,*inf,*outf,*pirf,*msff; + char *buff; + char *token; + char *charstring; + struct seqdat *bloc; + struct seqdat *temp; + int orientation; + extern int MAXtlen,MAXnbloc; + extern int MAXilen,precis,MAXslen; + int nseq; + int start_aa; + int start_seq; + int i,j,k; + int totalpage; + char **numbers; + char *ticks; + unsigned char **lines; +#ifdef VAX + char **fills; +#else + signed char **fills; +#endif + unsigned char **fonts; + unsigned char *idfonts; + unsigned char **inverse; + char ***texts; + int *Yindx; + int number_seqs = 0; + + int Xtemp,Ytemp; + int sx,sy,ex,ey; + + int fnum; + char *fname; + float fpoint; + int rowsiz; + + char oldchar,newchar; + + struct seqdat *addbloc; + struct addseq *adds; + int nadds; + int natot; + int l; + int do_ticks = 0; + int number_int = 10; + int pirsave = 0; + int msfsave = 0; + + int nbp,nspage; /*counter and total for number of blocks per page */ + int lastY; + int nbpage; + + /* bounding_box - allows explicit fiddling with the bounding_box */ + int *bounding_box; + + /* colours */ + float red,green,blue; + unsigned char **s_colour; /*colour number for shading */ + unsigned char **c_colour; /*colour number for characters */ + /* new 4th Oct 1994 */ + unsigned char *id_colour; /*colour for identifiers */ + unsigned char **left_line_colour; /*colours for lines */ + unsigned char **right_line_colour; + unsigned char **top_line_colour; + unsigned char **bottom_line_colour; + unsigned char **text_colour; /*colour for text */ + char *colname; + + int background_colour = 99; /*set background to white */ + int *background_region; /* use this to set the region for background colouring */ + + int number_colour = 100; /* colour for numbers along top of plot */ + /* end new */ + char *tstring; + + int use_colour; /* flag set if the define_colour command is seen */ + int res_numbers; + + int msl; + + /* min and max ranges in the sequence alignment */ + int xmin, ymin, xmax, ymax; + + /* masking variables */ + unsigned char **mask; + char *illegal; + char *legal; + int id_cut; + + /* screen size variable - for printer screen */ + int screensize = SCREENSIZE; + + /* set up relative numbering - allows commands to follow + a specific sequence numbering + firstres defines the number of the first residue */ + int relative_seq = 0; + int firstres = 0; + int i1; + int *relnum; + /* temp relnum */ + int *trelnum; + + /* flag for idents */ + int id_only_on_first = 0; + int tempCseqwidth; + int tempCidwidth; + + /* conservation values */ + int *consval; /* array */ + int conscut; /* cutoff */ + + /* counter for add_seq errors */ + int adderr; + + /* file streams */ + extern FILE *std_in, *std_out, *std_err; + + /* By default read and write to stdin and stdout */ + outf = std_out; + inf = std_in; + + + /* default is no colour */ + use_colour = 0; + + /* if set, residue numbers are output */ + res_numbers = 1; + + + + /* set the bounding box to default values for portrait A4*/ + bounding_box = (int *) GJmalloc(sizeof(int) * 4); + bounding_box[0] = 0; + bounding_box[1] = 0; + bounding_box[2] = 590; + bounding_box[3] = 826; + + /* set the background colour region equal to the default bounding box */ + background_region = (int *) GJmalloc(sizeof(int) * 4); + background_region[0] = 0; + background_region[1] = 0; + background_region[2] = 590; + background_region[3] = 826; + + /* initialise the mask */ + mask = NULL; + illegal = NULL; + legal = NULL; + + adds = (struct addseq *) malloc(sizeof(struct addseq) * (MAX_ADD_SEQ+1)); + nadds = 0; + + + fname = (char *) malloc(sizeof(char)*MAXtlen); + + buff = (char *) malloc(MAXtlen * sizeof(char)); + if(buff == NULL)error("No Space for buff",1); + + if((cf = fopen(command_file,"r")) == NULL){ + error("Cannot open ALSCRIPT command file",0); + return 0; + } + + /* + This is a little inelegant at the moment - when I get the generalized + parser finished this could be cleaned up substantially + */ + + /* main loop to read commands */ + if(pipe){ + /* Alscript is being used as a pipe so write preamble to std_out */ + outf = std_out; + PSPreamble(outf,singlepage,bounding_box,screensize); + } + + if(!silent)fprintf(std_err,"Starting ALSCRIPT\n"); + + while(buff = fgets(buff,MAXtlen,cf)){ + if(!silent)echo(buff); + if(buff == NULL) continue; + token = strtok(buff,TOKENS); + if(token == NULL) continue; + token = GJstoup(token); + if(*token == '#'){ + if(!silent)echo("Comment\n"); + }else if(strcmp(token,"SILENT_MODE")==0){ + if(!silent){ + silent=1; + }else{ + silent=0; + } + }else if(strcmp(token,"MAX_INPUT_LEN")==0){ + if(!silent) fprintf(std_err,"Max input len was:\t%d\n",MAXtlen); + token = strtok(NULL,TOKENS); + MAXtlen = atoi(token); + if(!silent)fprintf(std_err,"Max input len now:\t%d\n",MAXtlen); + }else if(strcmp(token,"MAX_NSEQ")==0){ + if(!silent)fprintf(std_err,"Max No. of sequences was:\t%d\n",MAXnbloc); + token = strtok(NULL,TOKENS); + MAXnbloc = atoi(token); + MAXtlen = MAXnbloc; + fprintf(std_err,"Max No. of sequences now:\t%d\n",MAXnbloc); + }else if(strcmp(token,"MAX_ILEN")==0){ + if(!silent)fprintf(std_err,"Max Identifier length was:\t%d\n",MAXilen); + token = strtok(NULL,TOKENS); + MAXilen = atoi(token); + if(!silent)fprintf(std_err,"Max Identifier length now:\t%d\n",MAXilen); + }else if(strcmp(token,"PRECISION")==0){ + token = strtok(NULL,TOKENS); + precis = atoi(token); + }else if(strcmp(token,"MAX_SEQ_LEN")==0){ + if(!silent)fprintf(std_err,"Max Sequence Length was:\t%d\n",MAXslen); + token = strtok(NULL,TOKENS); + MAXslen = atoi(token); + if(!silent)fprintf(std_err,"Max Sequence Length now:\t%d\n",MAXslen); + }else if(strcmp(token,"X_OFFSET")==0){ + if(!silent)fprintf(std_err,"X offset was:\t%d\n",xoff); + token = strtok(NULL,TOKENS); + xoff = atoi(token); + if(!silent)fprintf(std_err,"X offset now:\t%d\n",xoff); + }else if(strcmp(token,"Y_OFFSET")==0){ + if(!silent)fprintf(std_err,"Y offset was:\t%d\n",yoff); + token = strtok(NULL,TOKENS); + yoff = atoi(token); + if(!silent)fprintf(std_err,"Y offset now:\t%d\n",yoff); + }else if(strcmp(token,"MAX_SIDE")==0){ + if(!silent)fprintf(std_err,"Longest page side was:\t%.2f inches\n",MAXside/POINT); + token = strtok(NULL,TOKENS); + MAXside = POINT * atof(token); + if(!silent)fprintf(std_err,"Longest page side now:\t%.2f inches\n",MAXside/POINT); + }else if(strcmp(token,"MIN_SIDE")==0){ + if(!silent)fprintf(std_err,"Shortest page side was:\t%.2f inches\n",MINside/POINT); + token = strtok(NULL,TOKENS); + MINside = POINT * atof(token); + if(!silent)fprintf(std_err,"Shortest page side now:\t%.2f inches\n",MINside/POINT); + }else if(strcmp(token,"LINE_WIDTH_FACTOR")==0){ + if(!silent)fprintf(std_err,"Line width multiplication factor was:\t%f\n",LineWidthFactor); + token = strtok(NULL,TOKENS); + LineWidthFactor = atof(token); + if(!silent)fprintf(std_err,"Line width multiplication factor now:\t%f\n",LineWidthFactor); + }else if(strcmp(token,"SCREENSIZE")==0){ + if(!silent)fprintf(std_err,"Screensize was:\t%d\n",screensize); + token = strtok(NULL,TOKENS); + screensize = atoi(token); + if(!silent)fprintf(std_err,"Screensize now:\t%d\n",screensize); + }else if(strcmp(token,"BLOCK_FILE") == 0){ + token = strtok(NULL,TOKENS); + if(!pipe){ + inf = fopen(token,"r"); + if(inf == NULL)error("Cannot open BLOCK_FILE\n",1); + } + }else if(strcmp(token,"OUTPUT_FILE")==0){ + token = strtok(NULL,TOKENS); + if(!pipe){ + outf = fopen(token,"w"); + if(outf == NULL)error("Cannot open OUTPUT_FILE\n",1); + PSPreamble(outf,singlepage,bounding_box,screensize); + } + }else if(strcmp(token,"PIR_SAVE")==0){ + token = strtok(NULL,TOKENS); + pirf = fopen(token,"w"); + if(pirf == NULL)error("Cannot open PIR_SAVE file\n",1); + pirsave = 1; + }else if(strcmp(token,"MSF_SAVE")==0){ + token = strtok(NULL,TOKENS); + msff = fopen(token,"w"); + if(msff == NULL)error("Cannot open MSF_SAVE file\n",1); + msfsave = 1; + }else if(strcmp(token,"LANDSCAPE")==0){ + orientation = LANDSCAPE; + }else if(strcmp(token,"PORTRAIT")==0){ + orientation = PORTRAIT; + + }else if(strcmp(token,"POINTSIZE")==0){ + token = strtok(NULL,TOKENS); + pointsize = atof(token); + }else if(strcmp(token,"IDENT_WIDTH")==0){ + token = strtok(NULL,TOKENS); + Cidwidth = atoi(token); + if(Cidwidth > MAXilen)error("INCREASE MAX_ID_LEN value",1); + }else if(strcmp(token,"NUMBER_SEQS")==0){ + number_seqs=1; + }else if(strcmp(token,"X_SPACE_FACTOR")==0){ + if(!silent)fprintf(std_err,"Was:\t%.2f\n",XspaceFactor); + token = strtok(NULL,TOKENS); + XspaceFactor = atof(token); + if(!silent)fprintf(std_err,"Now Is:\t%.2f\n",XspaceFactor); + }else if(strcmp(token,"Y_SPACE_FACTOR")==0){ + if(!silent)fprintf(std_err,"Was:\t%.2f\n",YspaceFactor); + token = strtok(NULL,TOKENS); + YspaceFactor = atof(token); + }else if(strcmp(token,"X_SHIFT_FACTOR")==0){ + if(!silent)fprintf(std_err,"Was:\t%.2f\n",XshiftFactor); + token = strtok(NULL,TOKENS); + XshiftFactor = atof(token); + if(!silent)fprintf(std_err,"Now Is:\t%.2f\n",XshiftFactor); + }else if(strcmp(token,"Y_SHIFT_FACTOR")==0){ + if(!silent)fprintf(std_err,"Was:\t%.2f\n",YshiftFactor); + token = strtok(NULL,TOKENS); + YshiftFactor = atof(token); + if(!silent)fprintf(std_err,"Now Is:\t%.2f\n",YshiftFactor); + }else if(strcmp(token,"VERTICAL_SPACING")==0){ + if(!silent)fprintf(std_err,"Was:\t%d\n",Vspacing); + token = strtok(NULL,TOKENS); + Vspacing = (int) atol(token); + if(!silent)fprintf(std_err,"Now Is:\t%d\n",Vspacing); + }else if(strcmp(token,"DEFINE_FONT")==0){ + token = strtok(NULL,TOKENS); + fnum = atoi(token); /* font number */ + token = strtok(NULL,TOKENS); /* font name */ + fname = strcpy(fname,token); + token = strtok(NULL,TOKENS); + if(strcmp(token,"DEFAULT")==0){ + fpoint = pointsize; + }else if(strcmp(token,"REL")==0){ + token = strtok(NULL,TOKENS); + fpoint = pointsize * atof(token); + }else{ + fpoint = atof(token); + } + ALDefineFont(fnum,fname,fpoint,outf); + }else if(strcmp(token,"DEFINE_COLOUR")==0 || strcmp(token,"DEFINE_COLOR")==0){ + /* new style + define_colour colour_name r g b */ + /* e.g. define_colour 1 1.0 1.0 1.0 + (same as before) + define_colour rgb red 1.0 0.0 0.0 7 + (define an rgb colour (red,green,blue),colour_number) + define_colour hsb red 1.0 0.0 0.0 9 + (define an hsb colour (hue,sat,bright),colour_number) + currently can use either hsb OR rgb, but not mix them + */ + use_colour = 1; + token = strtok(NULL,TOKENS); + tstring = GJstoupper(token); + if(strcmp(tstring,"RGB")==0 || strcmp(tstring,"HSB")==0){ + token = strtok(NULL,TOKENS); + colname = GJstrdup(token); + token = strtok(NULL,TOKENS); + red = atof(token); + token = strtok(NULL,TOKENS); + green = atof(token); + token = strtok(NULL,TOKENS); + blue = atof(token); + token = strtok(NULL,TOKENS); + fnum = atoi(token); + }else{ + fnum = atoi(token); /* colour number */ + token = strtok(NULL,TOKENS); + red = atof(token); + token = strtok(NULL,TOKENS); + green = atof(token); + token = strtok(NULL,TOKENS); + blue = atof(token); + + } + /* for now just ignore the colour name - we'll allow use of this later */ + ALDefineColour(fnum,tstring,red,green,blue,outf); + GJfree(tstring); + }else if(strcmp(token,"ADD_SEQ")==0){ + token = strtok(NULL,TOKENS); + adds[nadds].pos = atoi(token); + token = strtok(NULL,TOKENS); + adds[nadds].num = atoi(token); + ++nadds; + }else if(strcmp(token,"DO_TICKS")==0){ + do_ticks=1; + }else if(strcmp(token,"NUMBER_INT")==0){ + token = strtok(NULL,TOKENS); + number_int = atoi(token); + if(!silent)fprintf(std_err,"Sequence Numbering interval now:\t%d\n",number_int); + }else if(strcmp(token,"NO_NUMBERS")==0){ + res_numbers = 0; + }else if(strcmp(token,"NUMBER_COLOUR")==0){ + token = strtok(NULL,TOKENS); + number_colour = atoi(token); + }else if(strcmp(token,"SINGLE_PAGE")==0){ + /* if this is set, then alscript assumes a single page - + just adds a bounding box for now*/ + if(!silent)fprintf(std_err,"Single page was: %d\n",singlepage); + singlepage = 1; + if(!silent)fprintf(std_err,"Single page now: %d\n",singlepage); + }else if(strcmp(token,"BOUNDING_BOX")==0){ + ALGetFourInt(&bounding_box[0],&bounding_box[1], + &bounding_box[2],&bounding_box[3]); + if(!silent){ + fprintf(std_err,"Bounding box set to: %d %d %d %d\n", + bounding_box[0],bounding_box[1], + bounding_box[2],bounding_box[3]); + } + }else if(strcmp(token,"BACKGROUND_REGION") == 0){ + /* set the region of the background to colour */ + ALGetFourInt(&background_region[0],&background_region[1], + &background_region[2],&background_region[3]); + if(!silent){ + fprintf(std_err,"Background region set to: %d %d %d %d\n", + background_region[0],background_region[1], + background_region[2],background_region[3]); + } + }else if(strcmp(token,"ID_ONLY_ON_FIRST_LINE")==0){ + /* flag to indicate identifiers only on first line!*/ + id_only_on_first = 1; + }else if(strcmp(token,"BACKGROUND_COLOUR") ==0){ + /* set the colour for background defined by background_region */ + token = strtok(NULL,TOKENS); + background_colour = atoi(token); + }else if(strcmp(token,"SETUP")==0){ + if(!silent)fprintf(std_err,"Initial Commands read - Establishing basic settings\n"); + break; + }else{ + fprintf(std_err,"%s",buff); + error("Unrecognised Step 1 command",1); + + } + } + + if(outf == std_out){ + if(!silent)fprintf(std_err,"Output file undefined - will write to stdout\n"); + } + + + if(inf == std_in){ + if(!silent)fprintf(std_err,"Block file undefined - will read from stdin\n"); + } + + bloc = (struct seqdat *) malloc(sizeof(struct seqdat) * MAXnbloc); + mcheck((char *) bloc,"Cannot get space for bloc"); + if(!Agetbloc(inf,bloc,&nseq))error("Cannot read bloc file",1); + + ckdd(bloc,nseq); + + if(pirsave){ + if(!save_pir(bloc,nseq,pirf))error("Cannot write to pirf file",1); + exit(0); + } + + if(msfsave){ + if(!save_msf(bloc,nseq,msff))error("Cannot write to msf file",1); + exit(0); + } + + /* Initialise relnum to 1..n*/ + /* relnum = (int *) GJmalloc(sizeof(int) * MAXslen); */ + /* for(i=1;i nseq){ + ++adderr; + fprintf(std_err,"Error in ADD_SEQ request: %d %d\n",adds[i].pos,adds[i].num); + fprintf(std_err,"%d is greater than the number of sequences in the block file(%d)\n",adds[i].pos,nseq); + fprintf(std_err,"Please see the manual for help on using ADD_SEQ\n"); + } + } + if(adderr > 0){ + fprintf(std_err,"%d ADD_SEQ errors - exiting\n",adderr); + exit(-1); + } + + + if(nadds > 0){ + if(!silent)fprintf(std_err,"Making extra space for %d additions\n",nadds); + natot = 0; + for(i=0;i bloc[1].slen-1) ex = bloc[1].slen-1; + ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax); + ALDoLine(lines,relnum[sx],sy,relnum[ex],LINE_TOP,Yindx); + }else if(strcmp(token,"BOTTOM")==0){ + ALGetThreeInt(&sx,&sy,&ex); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],sy,xmin,ymin,xmax,ymax); + if(ex > bloc[1].slen-1) ex = bloc[1].slen-1; + ALDoLine(lines,relnum[sx],sy,relnum[ex],LINE_BOTTOM,Yindx); + }else if(strcmp(token,"LEFT")==0){ + ALGetThreeInt(&sx,&sy,&ey); + ALCheckLegalRange(relnum[sx],sy,relnum[sx],ey,xmin,ymin,xmax,ymax); + if(ey > nseq) ey = nseq; + ALDoLine(lines,relnum[sx],sy,ey,LINE_LEFT,Yindx); + }else if(strcmp(token,"RIGHT")==0){ + ALGetThreeInt(&sx,&sy,&ey); + ALCheckLegalRange(relnum[sx],sy,relnum[sx],ey,xmin,ymin,xmax,ymax); + if(ey > nseq) ey = nseq; + ALDoLine(lines,relnum[sx],sy,ey,LINE_RIGHT,Yindx); + }else{ + error("Illegal LINE command\n",1); + } + }else if(strcmp(token,"BOX_REGION")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + ALBoxRegion(lines,relnum[sx],sy,relnum[ex],ey,Yindx); + }else if(strcmp(token,"SHADE_REGION")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALFilRegion(fills,relnum[sx],sy,relnum[ex],ey,Yindx,(float)atof(token)); + }else if(strcmp(token,"SHADE_RES")==0){ + ALGetTwoInt(&sx,&sy); + ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALFilRegion(fills,relnum[sx],sy,relnum[sx],sy,Yindx,(float)atof(token)); + }else if(strcmp(token,"COLOUR_REGION")==0 || strcmp(token,"COLOR_REGION")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALColRegion(s_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token)); + }else if(strcmp(token,"COLOUR_TEXT_REGION")==0 || strcmp(token,"COLOR_TEXT_REGION")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALColRRegion(text_colour,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token)); + }else if(strcmp(token,"COLOUR_RES")==0 || strcmp(token,"COLOR_RES")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetTwoInt(&sx,&sy); + ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALColRRegion(s_colour,relnum[sx],sy,relnum[sx],sy,(unsigned char) atoi(token)); + }else if(strcmp(token,"CCOLOUR_REGION")==0 || strcmp(token,"CCOLOR_REGION")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALColRRegion(c_colour,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token)); + }else if(strcmp(token,"COLOUR_LINE_REGION")==0 || strcmp(token,"COLOR_LINE_REGION")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALColRegion(left_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token)); + ALColRegion(right_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token)); + ALColRegion(top_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token)); + ALColRegion(bottom_line_colour,relnum[sx],sy,relnum[ex],ey,Yindx,(unsigned char) atoi(token)); + }else if(strcmp(token,"CCOLOUR_RES")==0 || strcmp(token,"CCOLOR_RES")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetTwoInt(&sx,&sy); + token = strtok(NULL,TOKENS); + ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax); + ALColRRegion(c_colour,relnum[sx],sy,relnum[sx],sy,(unsigned char) atoi(token)); + }else if(strcmp(token,"MASK")==0){ + /* mask commands */ + token = strtok(NULL,TOKENS); + if(strcmp(token,"SETUP")==0){ + if(mask == NULL){ + mask = uchararr(bloc[1].slen+1,nseq+1); + GJUCinit(mask,bloc[1].slen+1,nseq+1,0); + }else{ + GJUCinit(mask,bloc[1].slen+1,nseq+1,0); + } + }else if(strcmp(token,"RESET")==0){ + GJUCinit(mask,bloc[1].slen+1,nseq+1,0); + }else if(strcmp(token,"LEGAL")==0){ + /* get the legal character list */ + token = strtok(NULL,TOKENS); + if(legal != NULL) GJfree(legal); + legal = GJstrdup(token); + if(!silent)fprintf(stderr,"legal:%s\n",legal); + }else if(strcmp(token,"ILLEGAL")==0){ + /* get the illegal character list */ + token = strtok(NULL,TOKENS); + if(illegal != NULL) GJfree(illegal); + illegal = GJstrdup(token); + if(!silent)fprintf(stderr,"illegal:%s:\n",illegal); + }else if(strcmp(token,"ID")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + id_cut = atoi(token); + if(id_cut < (int) ( ( ((float) (ey-sy+1) / 2.0) + 0.6))){ + error("ID cutoff must be > half the number of sequences",1); + } + ALid_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,id_cut,legal,illegal); + }else if(strcmp(token,"AGREE")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token= strtok(NULL,TOKENS); + ALagree_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,atoi(token)); + }else if(strcmp(token,"FRE")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + ALfre_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,legal,illegal); + }else if(strcmp(token,"NOT")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + ALnot_mask(mask,relnum[sx],sy,relnum[ex],ey); + }else if(strcmp(token,"SUB")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token=strtok(NULL,TOKENS); + ALsub_mask(mask,bloc,relnum[sx],sy,relnum[ex],ey,token[0]); + }else if(strcmp(token,"REGION")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + ALMask(mask,relnum[sx],sy,relnum[ex],ey); + }else if(strcmp(token,"CONSERVATION")==0){ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token=strtok(NULL,TOKENS); + conscut = atoi(token); + if(!silent)fprintf(std_err,"Conservation Cutoff:%d\n",conscut); + ALConsMask(mask,relnum[sx],sy,relnum[ex],ey,consval,conscut); + }else if(strcmp(token,"BOX")==0){ + /* box the masked residues - ie surround them*/ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + ALSurroundMask(mask,bloc,lines,relnum[sx],sy,relnum[ex],ey,nseq); + }else if(strcmp(token,"SHADE")==0){ + /* shade the masked residues - */ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALShadeMask(mask,fills,relnum[sx],sy,relnum[ex],ey,Yindx,(float)atof(token)); + }else if(strcmp(token,"FONT")==0){ + /* set the font of the masked residues - */ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALFontMask(mask,fonts,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token)); + }else if(strcmp(token,"INVERSE")==0){ + /* invert the masked residues - */ + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALInverseMask(mask,inverse,relnum[sx],sy,relnum[ex],ey); + }else if(strcmp(token,"SCOL")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALSColMask(mask,s_colour,relnum[sx],sy,relnum[ex],ey,nseq,(unsigned char) atoi(token)); + }else if(strcmp(token,"CCOL")==0){ + if(!use_colour)error("You must use the DEFINE_COLOUR command first",1); + ALGetAllRange(&sx,&sy,&ex,&ey,xmax,nseq); + ALCheckLegalRange(relnum[sx],sy,relnum[ex],ey,xmin,ymin,xmax,ymax); + token = strtok(NULL,TOKENS); + ALColourMask(mask,c_colour,relnum[sx],sy,relnum[ex],ey,(unsigned char) atoi(token)); + }else{ + error("Unrecognised MASK qualifier\n",1); + } + }else if(strcmp(token,"TEXT")==0){ + token=strtok(NULL,TOKENS); + sx = atoi(token); + token=strtok(NULL,"\""); + sy = atoi(token); + token=strtok(NULL,"\""); + ALCheckLegalRange(relnum[sx],sy,relnum[sx],sy,xmin,ymin,xmax,ymax); + texts[relnum[sx]][sy] = (char *) malloc(sizeof(char)*(strlen(token)+1)); + texts[relnum[sx]][sy] = (char *) strcpy(texts[relnum[sx]][sy],token); + }else if(strcmp(token,"NUMBERS")==0){ + error("Numbers not implemented",1); + }else if(strcmp(token,"ID_FONT")==0){ + token = strtok(NULL,TOKENS); + if(strcmp(token,"ALL")==0){ + token = strtok(NULL,TOKENS); + for(i=1;i xmax ) xmax = k; + }else{ + for(i=1;i 0)){ + if(!silent)fprintf(std_err,"Saving (%d) Pages to file\n",totalpage/nspage); + }else{ + fprintf(std_err,"This pointsize is too large to print even a single character on the page\n"); + fprintf(std_err,"Try a smaller pointsize - or\n"); + fprintf(std_err,"Try reducing the Identifier width\n"); + fprintf(std_err,"Increase the paper size\n"); + fprintf(std_err,"You could do some PostScrip-ery to print partial characters\n"); + fprintf(std_err,"After creating an alignment on a single page - see the CookBook\n"); + exit(1); + } + lastY = 0; + nbpage = 0; + k = 0; + start_seq = 1; + while(k < totalpage){ + start_aa = 1; + Ytemp = start_seq+Cheight-CtopSpace - 1; + if(Ytemp > nseq) Ytemp = nseq; + for(i=0;i 1){ /* more than one block per page */ +/* fprintf(stderr,"nbp nspage %d %d\n",nbp,nspage);*/ + if(k>0 && nbp == 0){ /* if not the first page, then setup page*/ + PSSetupPage(orientation,nbpage+1,outf); + if(background_colour != 99){ + ALColourBackground(outf,background_colour,background_region); + } + } + if(nbp>0){ + /* if this is not the first block on the page, then */ + /* translate the block of alignment back to origin */ + fprintf(outf,"%d %d translate\n",0,-lastY); + } + /* get new position for drawing and translate to this posn*/ + lastY = Yspace*( Cheight-(nseq+CtopSpace)*(nbp+1) - (nbp*Vspacing) ); + fprintf(outf,"%d %d translate\n",0,lastY); + }else{ + if(k>0)PSSetupPage(orientation,k+1,outf); + if(background_colour != 99){ + ALColourBackground(outf,background_colour,background_region); + } + } + if(id_only_on_first == 1 && nbp > 0 ){ + /* save the Cseqwidth, then reset it to Cwidth - also set Cidwidth to 0*/ + tempCseqwidth = Cseqwidth; + tempCidwidth = Cidwidth; + Cseqwidth = Cwidth; + Cidwidth = 0; + } + + Xtemp = start_aa+Cseqwidth-1; + if(Xtemp > bloc[1].slen) Xtemp = bloc[1].slen; + + fprintf(outf,"2 setlinecap\n"); + fprintf(outf," %.2f setlinewidth\n",LineWidthFactor*pointsize); + + ALDrawFills(fills,use_colour,s_colour,start_aa,nseq-Ytemp+1,Xtemp,nseq-start_seq+1,Cidwidth,CseqVoffset,outf); + ALDrawLines(lines,use_colour, + left_line_colour, + right_line_colour, + top_line_colour, + bottom_line_colour, + start_aa,nseq-Ytemp+1,Xtemp,nseq-start_seq+1,Cidwidth,CseqVoffset,outf); + if(!(id_only_on_first == 1 && (nbp > 0 || k > 0))){ + /* don't output ID string if this is not the first block on the first page*/ + ALOutId(bloc,use_colour, + id_colour,idfonts,start_seq,Ytemp,CseqVoffset,number_seqs,outf); + } + ALOutSeq(bloc,fonts,texts,inverse,use_colour,c_colour, + text_colour,background_colour,start_seq,Ytemp,start_aa,CseqVoffset,outf); + + if(res_numbers){ + if(use_colour)PSSetColour(number_colour,outf); + if(do_ticks){ + ALOutTicks(ticks,start_aa,Cidwidth,Ytemp-start_seq+1,bloc[1].slen,outf); + ALOutNumbers(numbers,start_aa,Cidwidth,Ytemp-start_seq+2,bloc[1].slen,outf); + }else{ + ALOutNumbers(numbers,start_aa,Cidwidth,Ytemp-start_seq+1,bloc[1].slen,outf); + } + } + start_aa += Cseqwidth; + + if(nspage > 1){ + ++nbp; + if(nbp == nspage || k == totalpage-1){ /* put showpage command for page or last page */ + PSShowPage(outf); + nbp = 0; + ++nbpage; + } + }else{ + PSShowPage(outf); + } + ++k; + } + start_seq = Ytemp + 1; + if(id_only_on_first == 1 && nbp > 0){ + /* reset the values - not strictly necessary */ + Cseqwidth = tempCseqwidth; + Cidwidth = tempCidwidth; + } + } + return 1; +} + +void ALShadeChars(struct seqdat *bloc, +signed char **fill, +int start_x,int start_y,int end_x,int end_y, +int maxY, +char *charlist, +float grey) + +/* shade residues in charlist by grey value */ + +{ + int i,j,jpos; + for(i=start_x;i= 0 draw box and fill in with grey value */ +/* 6/12/92: add colour option */ +{ + int i,j; + extern int precis; + + XOffset *= Xspace; + YOffset *= Yspace; + + for(i=end_x;i>(start_x-1);--i){ + for(j=end_y;j>(start_y-1);--j){ + if((int) fill[i][j] >= 0 || use_colour){ + +/* +Revise to use ML function 27th Oct 1992 + fprintf(outf," newpath\n"); + fprintf(outf,"%.2f %.2f moveto \n",(float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset); + fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset); + fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset); + fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset); + fprintf(outf,"%.2f %.2f lineto \n",(float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset); + fprintf(outf,"closepath %.2f setgray fill 0 setgray \n",(float)fill[i][j]/(float)precis); +*/ + fprintf(outf," newpath\n"); + fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f ML\n", + (float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset, + (float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset, + (float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset, + (float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset, + (float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset); + if(use_colour && fill[i][j] < 0){ + /* 6/1/1995 - only set colour if grey is not set */ + /* set to colour number */ + fprintf(outf,"closepath C%-d fill \n",colour[i][j]); + }else{ + fprintf(outf,"closepath %.2f setgray fill 0 setgray \n",(float)fill[i][j]/(float)precis); + } + } + } + } +} + +void ALFilRes(signed char **fill,int i,int j,float grey) + +{ + extern int precis; +/* ALBoxRes(lines,i,j); + fill[i+Cidwidth][j+CseqVoffset] = grey * precis; +*/ + fill[i][j] = grey * precis; +} + +void ALColRes(unsigned char **colour,int i,int j,int colnum) + +{ + colour[i][j] = colnum; +} + + +void ALBoxRes(unsigned char **lines,int i,int j) + +{ + ALLineRes(lines,i,j,LINE_TOP); + ALLineRes(lines,i,j,LINE_BOTTOM); + ALLineRes(lines,i,j,LINE_LEFT); + ALLineRes(lines,i,j,LINE_RIGHT); +} + + +void ALLineRes( +unsigned char **lines, +int i,int j, +unsigned char value) +{ +/* + i = i + Cidwidth; + j = j + CseqVoffset; +*/ + ALLineChar(lines,i,j,value); +} + +void ALLineChar(unsigned char **lines,int i,int j,unsigned char value) + +{ + lines[i][j] = lines[i][j] | value; +} + +void ALDrawLines(unsigned char **lines,int use_colour, + unsigned char **left_line_colour, + unsigned char **right_line_colour, + unsigned char **top_line_colour, + unsigned char **bottom_line_colour, + int start_x,int start_y,int end_x,int end_y, + int XOffset,int YOffset,FILE *outf) + +/* draw the lines that are indicated by the lines array in the range of the +array indicated by start_x etc +*/ +{ + int i,j; + XOffset *= Xspace; + YOffset *= Yspace; + +/* + if(use_colour){ + fprintf(outf,"C100\n"); + } +*/ + + for(i=end_x;i>(start_x-1);--i){ + for(j=end_y;j>(start_y-1);--j){ + if(lines[i][j] != 0){ + if((LINE_LEFT & lines[i][j]) == LINE_LEFT){ + PSSetColour(left_line_colour[i][j],outf); + PSline((float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset, + (float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset,outf); + } + if((LINE_RIGHT & lines[i][j]) == LINE_RIGHT){ + PSSetColour(right_line_colour[i][j],outf); + PSline((float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset, + (float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset,outf); + } + if((LINE_TOP & lines[i][j]) == LINE_TOP){ + PSSetColour(top_line_colour[i][j],outf); + PSline((float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset, + (float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+1)*Yspace-Yshift+YOffset,outf); + } + if((LINE_BOTTOM & lines[i][j]) == LINE_BOTTOM){ + PSSetColour(bottom_line_colour[i][j],outf); + PSline((float)(i-start_x)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset, + (float)(i-start_x+1)*Xspace-Xshift+XOffset, + (float)(j-start_y+0)*Yspace-Yshift+YOffset,outf); + } + } + } + } + /* + if(use_colour){ + fprintf(outf,"C99\n"); + } + */ +} + +void PSline(float x1,float y1,float x2,float y2,FILE *outf) + +{ + fprintf(outf,"%.2f %.2f %.2f %.2f L\n ",x1,y1,x2,y2); +} + + + +void ALSimpleBox(int x1,int y1,int x2,int y2,float pfactor, /* multiply pointsize by this to get linewidth */ + FILE *outf) + +/* draw a box to include character coordinates x1,y1 to x2,y2 */ +/* x1 < x2 y1< y2 */ +{ + float lshift = Xspace/6; + float rshift = Xspace - lshift; + + x1 *= Xspace; + y1 *= Yspace; + x2 *= Xspace; + y2 *= Yspace; + + x1 -= lshift; + x2 += rshift; + y1 -= lshift; + + PSDrawBox((float)x1,(float)y1,(float)x2,(float)y2,pfactor,outf); +} + +void PSDrawBox(float x1,float y1,float x2,float y2,float pfactor,FILE *out) + +{ + fprintf(out,"%f setlinewidth\n ",pointsize*pfactor); + fprintf(out,"%f %f moveto %f %f lineto %f %f lineto %f %f lineto %f %f lineto closepath stroke\n", + x1,y1,x2,y1,x2,y2,x1,y2,x1,y1); +} + +void ALOutTicks(char *array,int start_aa,int xpos,int ypos,int len,FILE *out) + +{ + int right; + int j; + + xpos *= Xspace; + ypos *= Yspace; + + right = start_aa + Cseqwidth; + if(right > len)right = len; + for(j=start_aa;j len)right = len; + for(j=start_aa;j bloc[1].slen-1)right = bloc[1].slen; + + for(i=end_seq;i>(start_seq-1);--i){ + xpos = Cidwidth * Xspace; + for(j=start_aa;j(start_seq-1);--i){ + if(use_colour)PSSetColour(colour[i],outf); + if(fonts[i] != 0)PSSetFont(fonts[i],outf); + if(numbers){ + sprintf(temp,"%4d:%s",i,bloc[i].id); + }else{ + sprintf(temp,"%s",bloc[i].id); + } + PSPutText(xpos,ypos,temp,outf); + ypos += Yspace; + if(fonts[i] != 0)PSSetFont(0,outf); + } + free((char *) temp); + +} + +void PSPutText(int x,int y,char *text,FILE *outf) + +{ + fprintf(outf," %d %d moveto (%s) show \n",x,y,text); +} + +void PSPutChar(int x,int y,char c,FILE *outf) + +{ + /* fprintf(outf," %d %d moveto (%c) show \n",x,y,c);*/ + fprintf(outf,"(%c) %d %d P\n",c,x,y); +} + +void PSShowPage(FILE *outf) + +{ + fprintf(outf,"showpage\n"); +} + +void ALSetFont(char *font,int point,FILE *outf) + +{ + fprintf(outf,"/%s findfont %d scalefont setfont\n",font,point); +} + +void PSSetFont(unsigned char number,FILE *outf) + +{ + fprintf(outf,"F%-d\n ",number); +} + +void PSSetColour(unsigned char number,FILE *outf) + +{ + fprintf(outf,"C%-d\n ",number); +} + +void ALDefineFont(int number,char *font,float point,FILE *outf) + +/* define a PostScript function called /Fnumber which selects the +font at point pointsize */ +{ + fprintf(outf,"/F%-d {/%s findfont %.2f scalefont setfont } def\n", + number,font,point); +} + +void ALDefineColour(int number,char *type,float red,float green,float blue,FILE *outf) + +/* define a PostScript function called /Cnumber which selects the +colour defined by red,green, blue intensities + +new addition - if type is equal to "HSB" then define an hsb colour using red, green blue as +the hue saturation and brightness. +*/ +{ + if(strcmp(type,"HSB")==0){ + /* set an HSB colour */ + fprintf(outf,"/C%-d {%f %f %f sethsbcolor } def\n", number,red,green,blue); + }else{ + /* set an RGB colour */ + fprintf(outf,"/C%-d {%f %f %f setrgbcolor } def\n", number,red,green,blue); + } +} + +void ALCheckSinglePage(int nseq,int len,int silent) + +{ + extern FILE *std_err; + Csheet = 1; + + + if(Cheight-CtopSpace < nseq){ + if(!silent)fprintf(std_err,"Not enough height to print all sequences on a single page at this pointsize\n"); + if(!silent)fprintf(std_err,"Pointsize: %.2f\n",pointsize); + if(!silent)fprintf(std_err,"Need a pointsize of < : %.2f\n",(float) height/(nseq+ CtopSpace)); + Csheet = nseq/(Cheight-CtopSpace) + 1; + if(!silent)fprintf(std_err,"Will split alignment into %d segments\n",Csheet); + } + + npage = len/(Cwidth - Cidwidth) + 1; +} + + +void ALSetPageLimits(void) +/* Use the current width height and pointsize values to calculate: + Cwidth + Cheight + Xspace + Yspace +*/ +{ + + Xspace = pointsize + pointsize * XspaceFactor; + Yspace = pointsize + pointsize * YspaceFactor; + + Cwidth = width/Xspace; + Cheight = height/Yspace; + + Cseqwidth = Cwidth - Cidwidth; + + Xshift = Xspace * XshiftFactor; + Yshift = Yspace * YshiftFactor; +} + +void PSSetOrientation(int orientation,FILE *outf) + +{ + switch(orientation){ + case LANDSCAPE: + PSLandscape(outf); + break; + case PORTRAIT: + PSPortrait(outf); + break; + default: + PSPortrait(outf); + } +} + +void PSPreamble(FILE *out,int singlepage,int *b_box,int screensize) +{ + char *Alscript_Version = ALSCRIPT_VERSION; + fprintf(out,"%%!PS-Adobe-2.0\n"); + fprintf(out,"%%%%Creator: %s\n",Alscript_Version); + fprintf(out,"%%%%Title: Alscript Output\n"); + fprintf(out,"%% Barto,n G. J. (1993)\n"); + fprintf(out,"%% ALSCRIPT - A Tool to format multiple sequence alignments\n"); + fprintf(out,"%% Protein Engineering, 6, 37-40\n"); + fprintf(out,"%%\n"); + if(singlepage == 1){ + fprintf(out,"%%%%BoundingBox: %d %d %d %d\n", + b_box[0],b_box[1],b_box[2],b_box[3]); + } + fprintf(out,"/P {moveto show} def\n"); + fprintf(out,"/L {moveto lineto stroke} def\n"); + fprintf(out, + "/ML {moveto lineto lineto lineto lineto} def\n"); + fprintf(out,"2 setlinecap\n"); /* projecting line caps */ + ALDefineColour(99,"RGB",1.0,1.0,1.0,out); /* white */ + ALDefineColour(100,"RGB",0.0,0.0,0.0,out); /* black */ + /* modify the screening - suggested by Michael D. Baron */ + fprintf(out,"%d currentscreen 3 -1 roll pop setscreen\n",screensize); + +} + +void PSLandscape(FILE *out) + +{ + width = MAXside; + height = MINside; + + fprintf(out,"%d %d translate\n",xoff+height,yoff); + fprintf(out,"90 rotate\n"); + +} + +void PSPortrait(FILE *out) + +{ + width = MINside; + height = MAXside; + fprintf(out,"%d %d translate\n",xoff,yoff); +} + + +void echo(char *s) + +{ + extern FILE *std_err; + fprintf(std_err,"%s",s); +} + +void ALGetFourInt(int *sx,int *sy,int *ex,int *ey) + +{ +char *token; + + token = strtok(NULL,TOKENS); + *sx = atoi(token); + token = strtok(NULL,TOKENS); + *sy = atoi(token); + token = strtok(NULL,TOKENS); + *ex = atoi(token); + token = strtok(NULL,TOKENS); + *ey = atoi(token); +} +void ALGetThreeInt(int *sy,int *ex,int *ey) + +{ +char *token; + + token = strtok(NULL,TOKENS); + *sy = atoi(token); + token = strtok(NULL,TOKENS); + *ex = atoi(token); + token = strtok(NULL,TOKENS); + *ey = atoi(token); +} +void ALGetTwoInt(int *sy,int *ex) + +{ +char *token; + + token = strtok(NULL,TOKENS); + *sy = atoi(token); + token = strtok(NULL,TOKENS); + *ex = atoi(token); +} + +char ALChekSpace(const char *token) +{ + if(strcmp(token,"SPACE") == 0){ + return ' '; + }else{ + return token[0]; + } +} + +void PSSetGrey(float grey,FILE *outf) +{ + fprintf(outf," %.2f setgray\n",grey); +} + +int save_pir(struct seqdat *bloc,int nseq,FILE *pirf) + +{ + int i,j; + int count; + + + for(i=1;i<(nseq+1);++i){ + fprintf(pirf,">P1;%s\n",bloc[i].id); + fprintf(pirf,"%s\n",bloc[i].title); + count=0; + for(j=1;j bloc[1].slen) e = bloc[1].slen; + + for(i=1;i<(nseq+1);++i){ + fprintf(msff,"%10s ",bloc[i].id); + for(j=s;j dex) *ex = dex; + if(*ey > dey) *ey = dey; +} + +void ALid_mask( +unsigned char **mask, +struct seqdat *bloc, +int sx,int sy,int ex,int ey, +int id_cut, +char *legal, +char *illegal) + +{ + int i,j,k,iseen; + char fchar; + char *seen; /* array of characters that are seen at this position */ + int *freq; /* array of frequencies of the characters */ + int nc; /* number of different character types at this position */ + int ns; + int mc; /* maximum character present */ + int imc; /* location of max character */ + + ns = ey - sy + 1; + nc = 0; + + seen = (char *) GJmalloc(sizeof(char) *ns); + freq = (int *) GJmalloc(sizeof(int) *ns); + + for(i=0;i= 0){ + ++freq[iseen]; + }else{ + seen[nc] = bloc[j].seq[i]; + ++freq[nc]; + ++nc; + } + } + /* + for(k=0;k mc){ + mc = freq[k]; + imc = k; + } + }else if(legal != NULL){ + if( strchr(legal,seen[k]) != NULL + && freq[k] > mc){ + mc = freq[k]; + imc = k; + } + }else if(illegal != NULL){ + if( strchr(illegal,seen[k]) == NULL + && freq[k] > mc){ + mc = freq[k]; + imc = k; + } + }else{ + if(freq[k] > mc){ + mc = freq[k]; + imc = k; + } + } + } + if(mc >= id_cut){ + /* only select the character if it is >= id_cut */ + fchar = seen[imc]; +/* fprintf(stdout,"At %d %c\n",i,fchar);*/ + for(j=sy;j<(ey+1);++j){ + if(bloc[j].seq[i] == fchar){ + mask[i][j] = 1; +/* fprintf(stdout,"%d %d \n",i,j);*/ + } + } + } + for(j=0;j= 0){ + ++freq[iseen]; + }else{ + seen[nc] = bloc[j].seq[i]; + ++freq[nc]; + ++nc; + } + } +/* for(k=0;k mc){ + mc = freq[k]; + imc = k; + } + }else if(legal != NULL){ + if( strchr(legal,seen[k]) != NULL + && freq[k] > mc){ + mc = freq[k]; + imc = k; + } + }else if(illegal != NULL){ + if( strchr(illegal,seen[k]) == NULL + && freq[k] > mc){ + mc = freq[k]; + imc = k; + } + }else{ + if(freq[k] > mc){ + mc = freq[k]; + imc = k; + } + } + } + fchar = seen[imc]; +/* fprintf(stdout,"At %d %c\n",i,fchar);*/ + for(j=sy;j<(ey+1);++j){ + if(bloc[j].seq[i] == fchar){ + mask[i][j] = 1; +/* fprintf(stdout,"%d %d \n",i,j);*/ + } + } + + for(j=0;j 0){ + for(k=0;k= conscut in the consval array */ + for(j=sy;j<(ey+1);++j){ + if(consval[i] >= conscut){ + mask[i][j] = 1; + } + } + } +} + + +void ALDrawHorizLine( + float Xpos, /* bottom left hand corner of character posn */ + float Ypos, /* */ + float Xspace, /* horizontal width of character position */ + float Yspace, /* vertical height of character position */ + float width_fac,FILE *outf) /* proportion of vertical height that the line will fill */ + + +{ + float xright,ytop; + float ymid; + float hline_w; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + + /* width_fac defines the width of the line in terms of the height of the characters */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f %.2f ML\n", + Xpos,ymid-hline_w, + xright,ymid-hline_w, + xright,ymid+hline_w, + Xpos,ymid+hline_w, + Xpos,ymid-hline_w,outf); +} + +void ALDrawRightArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + + /* width_fac defines the width of the Arrow in terms of the height of the characters */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSmoveto(Xpos,Ypos,outf); + PSlineto(xright,ymid,outf); + PSlineto(Xpos,ytop,outf); + PSlineto(Xpos,Ypos,outf); + +} + +void ALDrawLeftArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + + /* width_fac defines the width of the Arrow in terms of the height of the characters */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSmoveto(xright,Ypos,outf); + PSlineto(xright,ytop,outf); + PSlineto(Xpos,ymid,outf); + PSlineto(xright,Ypos,outf); +} + + +void ALDrawUpArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSmoveto(Xpos,Ypos,outf); + PSlineto(xright,Ypos,outf); + PSlineto(xmid,ytop,outf); + PSlineto(Xpos,Ypos,outf); +} + +void ALDrawDownArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSmoveto(Xpos,ytop,outf); + PSlineto(xright,ytop,outf); + PSlineto(xmid,Ypos,outf); + PSlineto(Xpos,ytop,outf); +} + +/* Draw a small up arrow. mok 980917 */ +void ALDrawSmallUpArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float xmid; + float x,y; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + PSmoveto(xmid, ytop, outf); + x = Xpos + (xright-Xpos)/5.0; y = Ypos + 2.0*(ytop-Ypos)/3.0; + PSlineto(x,y,outf); + x = Xpos + 2.0*(xright-Xpos)/5.0; + PSlineto(x, y, outf); + PSlineto (x, Ypos, outf); + x = Xpos + 3.0*(xright-Xpos)/5.0; + PSlineto (x, Ypos, outf); + PSlineto(x, y, outf); + x = Xpos + 4.0*(xright-Xpos)/5.0; + PSlineto(x, y, outf); + PSlineto(xmid, ytop, outf); +} + +/* Draw a small down arrow. mok 980917 */ +void ALDrawSmallDownArrow(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float xmid; + float x,y; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + PSmoveto(xmid, Ypos, outf); + x = Xpos + 4.0*(xright-Xpos)/5.0; y = Ypos + (ytop-Ypos)/3.0; + PSlineto(x,y,outf); + x = Xpos + 3.0*(xright-Xpos)/5.0; + PSlineto(x, y, outf); + PSlineto (x, ytop, outf); + x = Xpos + 2.0*(xright-Xpos)/5.0; + PSlineto (x, ytop, outf); + PSlineto(x, y, outf); + x = Xpos + (xright-Xpos)/5.0; + PSlineto(x, y, outf); + PSlineto(xmid, Ypos, outf); +} + +void ALDrawCircle(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSnewpath(outf); + + fprintf(outf,"%.2f %.2f %.2f %d %d arc\n",xmid,ymid,(ytop - Ypos)*0.5,0,360); + +} + +/* Draw a diamond. mok 980917 */ +void ALDrawDiamond(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + PSmoveto(Xpos, ymid, outf); + PSlineto(xmid, ytop, outf); + PSlineto(xright, ymid, outf); + PSlineto(xmid, Ypos, outf); + PSlineto(Xpos, ymid, outf); +} + +/* Draw a bar. mok 980917 */ +void ALDrawBar(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright; + float y1, y2; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + + y1 = Ypos + 2.0*(Ypos+Yspace)/5.0; + y2 = Ypos + 3.0*(Ypos+Yspace)/5.0; + + PSmoveto(Xpos, y1, outf); + PSlineto(xright, y1, outf); + PSlineto(xright, y2, outf); + PSlineto(Xpos, y2, outf); + PSlineto(Xpos, y1, outf); +} + +void ALDrawPseudoEllipse(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac, + FILE *outf) + +{ + /* draws a pseudo ellipse in the box bounded by Xpos,Ypos, Xpos+Xspace, Ypos+Yspace */ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + float xefact = 0.2; /* this shifts the guide points by 0.2 towards the axis */ + /* should not really be hard wired */ + float yefact = 0.0; + + float xbit,ybit; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + xbit = Xspace * xefact; + ybit = Yspace * yefact; + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSnewpath(outf); + + PSmoveto(xmid,Ypos,outf); + + fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f curveto\n", + Xpos+xbit,Ypos+ybit,Xpos+xbit,ytop-ybit,xmid,ytop); + fprintf(outf,"%.2f %.2f %.2f %.2f %.2f %.2f curveto\n", + xright-xbit,ytop-ybit,xright-xbit,Ypos+ybit,xmid,Ypos); +} + +void ALDrawLeftSemiEnd(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSnewpath(outf); + + fprintf(outf,"%.2f %.2f %.2f %d %d arc\n",xmid,ymid,(ytop - Ypos) *0.5,270,90); + + PSlineto(xright,ytop,outf); + PSlineto(xright,Ypos,outf); + PSlineto(xmid,Ypos,outf); + +} +void ALDrawRightSemiEnd(float Xpos,float Ypos, float Xspace, float Yspace, float width_fac,FILE *outf) + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSnewpath(outf); + fprintf(outf,"%f %f %f %d %d arc\n",xmid,ymid,(ytop - Ypos) *0.5,90,270); + + PSlineto(Xpos,Ypos,outf); + PSlineto(Xpos,ytop,outf); + PSlineto(xmid,ytop,outf); +} +void ALDrawRightHalfHorizLine( + float Xpos, /* bottom left hand corner of character posn */ + float Ypos, /* */ + float Xspace, /* horizontal width of character position */ + float Yspace, /* vertical height of character position */ + float width_fac, FILE *outf) /* proportion of vertical height that the line will fill */ + +/* draw line from midpoint to right of position */ + + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + /* width_fac defines the width of the line in terms of the height of the characters */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSmoveto(xmid,ymid-hline_w,outf); + PSlineto(xmid,ymid+hline_w,outf); + PSlineto(xright,ymid+hline_w,outf); + PSlineto(xright,ymid-hline_w,outf); + PSlineto(xmid,ymid-hline_w,outf); +} + +void ALDrawLeftHalfHorizLine( + float Xpos, /* bottom left hand corner of character posn */ + float Ypos, /* */ + float Xspace, /* horizontal width of character position */ + float Yspace, /* vertical height of character position */ + float width_fac, FILE *outf) /* proportion of vertical height that the line will fill */ + +/* draw line from midpoint to left of position */ + +{ + float xright,ytop; + float ymid; + float hline_w; + float xmid; + + xright = Xpos + Xspace; /* right hand edge of character (lhe of next character) */ + ytop = Ypos + Yspace; /* top edge of character */ + ymid = (ytop + Ypos) * 0.5; /* vertical mid point */ + xmid = (xright + Xpos) * 0.5; /* horizontal mid point */ + + /* width_fac defines the width of the line in terms of the height of the characters */ + + hline_w = 0.5 * (width_fac * (ytop - Ypos)); + + PSmoveto(xmid,ymid-hline_w,outf); + PSlineto(xmid,ymid+hline_w,outf); + PSlineto(Xpos,ymid+hline_w,outf); + PSlineto(Xpos,ymid-hline_w,outf); + PSlineto(xmid,ymid-hline_w,outf); +} + +void PSmoveto(float x,float y,FILE *outf) +{ + fprintf(outf,"%.2f %.2f moveto\n",x,y); +} +void PSlineto(float x,float y,FILE *outf) +{ + fprintf(outf,"%.2f %.2f lineto\n",x,y); +} +void PSPutSpecialText(float Xpos,float Ypos,float Xspace,float Yspace,char *text, + unsigned char current_col, + unsigned char background_colour,float YspaceFactor,FILE *outf) +/* Handle the special text characters */ +{ + + Yspace *= YspaceFactor; /* shrink the Y axis a little to avoid clashes.*/ + + + if(strcmp(text,"@horizline") == 0){ + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fhorizline") == 0){ + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@thickhorizline") == 0){ + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + PSstroke(outf); + }else if(strcmp(text,"@fthickhorizline") == 0){ + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + PSstroke(outf); + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@thinhorizline") == 0){ + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + PSstroke(outf); + }else if(strcmp(text,"@fthinhorizline") == 0){ + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + PSstroke(outf); + ALDrawHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@rightarrow") == 0){ + ALDrawRightArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@frightarrow") == 0){ +/* ALDrawRightArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf);*/ +/* temporary change to the right arrow width 20/1/1995*/ + ALDrawRightArrow(Xpos,Ypos,(Xspace*0.7),Yspace,0.5,outf); + PSstroke(outf); + ALDrawRightArrow(Xpos,Ypos,(Xspace*0.7),Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@leftarrow") == 0){ + ALDrawLeftArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fleftarrow") == 0){ + ALDrawLeftArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawLeftArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@uparrow") == 0){ + ALDrawUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fuparrow") == 0){ + ALDrawUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@suparrow") == 0){ + ALDrawSmallUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fsuparrow") == 0){ + ALDrawSmallUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawSmallUpArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@downarrow") == 0){ + ALDrawDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fdownarrow") == 0){ + ALDrawDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@sdownarrow") == 0){ + ALDrawSmallDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fsdownarrow") == 0){ + ALDrawSmallDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawSmallDownArrow(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@circle") == 0){ + ALDrawCircle(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fcircle") == 0){ + ALDrawCircle(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawCircle(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@diamond") == 0){ + ALDrawDiamond(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fdiamond") == 0){ + ALDrawDiamond(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawDiamond(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@bar") == 0){ + ALDrawBar(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fbar") == 0){ + ALDrawBar(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawBar(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@leftsemiend") == 0){ + ALDrawLeftSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@fleftsemiend") == 0){ + ALDrawLeftSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawLeftSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@rightsemiend") == 0){ + ALDrawRightSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + }else if(strcmp(text,"@frightsemiend") == 0){ + ALDrawRightSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + ALDrawRightSemiEnd(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + }else if(strcmp(text,"@lefthelixend") == 0){ + + ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + PSstroke(outf); + ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,0.5,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",background_colour); + + ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + PSstroke(outf); + ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,0.5,outf); + PSstroke(outf); + + }else if(strcmp(text,"@righthelixend") == 0){ + + ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + PSstroke(outf); + ALDrawLeftHalfHorizLine(Xpos,Ypos,Xspace,Yspace,1.0,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,1.0,outf); + PSstroke(outf); + ALDrawPseudoEllipse(Xpos,Ypos,Xspace,Yspace,1.0,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + PSstroke(outf); + ALDrawRightHalfHorizLine(Xpos,Ypos,Xspace,Yspace,0.1,outf); + fprintf(outf,"closepath \n C%-d \n fill \n",current_col); + + + }else{ + fprintf(stderr,"Error: Unrecognised special character: %s\n",text); + error(" ",1); + } +} + +void PSstroke(FILE *outf) +{ + fprintf(outf,"stroke\n"); +} + +void PSnewpath(FILE *outf) +{ + fprintf(outf,"newpath\n"); +} + +void ALColourBackground(FILE *outf,int colour, int *b_region) +{ + int x1,y1,x2,y2; + x1 = b_region[0]; + x2 = b_region[2]; + y1 = b_region[1]; + y2 = b_region[3]; + + fprintf(outf,"%d %d moveto %d %d lineto %d %d lineto %d %d lineto %d %d lineto closepath\n", + x1,y1,x2,y1,x2,y2,x1,y2,x1,y1); + fprintf(outf," C%-d \n fill \n",colour); + /* reset the colour to white */ + /* fprintf(outf," C99\n");*/ +} + +void ALCheckLegalRange(int sx,int sy, int ex, int ey, int xmin, int ymin, int xmax, int ymax) +/* check if sx, sy, ex, ey are within the limits given by xmin, ymin, xmax, ymax */ +{ + extern FILE *std_err; + if( sx >= xmin && sx <= xmax + && sy >= ymin && sy <= ymax + && ex <= xmax && ex >= xmin + && ey <= ymax && ey >= ymin){ + return; + }else{ + fprintf(std_err,"\n\nALSCRIPT COMMAND FILE ERROR:\n"); + fprintf(std_err,"Maximum legal range:\n"); + fprintf(std_err,"From position %d of sequence %d to position %d of sequence %d\n", + xmin,ymin,xmax,ymax); + + fprintf(std_err,"Your range:\n"); + fprintf(std_err,"From position %d of sequence %d to position %d of sequence %d\n", + sx,sy,ex,ey); + + GJerror("Range too big for alignment - try again!"); + exit(0); + } +} + +void ALMakeHelixText(int sx, int sy, int ex, char ***texts) +{ + int i; + texts[sx][sy] = GJstrdup("@lefthelixend"); + texts[ex][sy] = GJstrdup("@righthelixend"); + for(i=sx+1;i has same effect as 1, But does not prompt for the command file + e.g. alscript example1.com + +3. alscript -q < > + Quick mode - uses default commands, reads alignment from stdin, writes PostScript + to stdout. + e.g. alscript -q < example1.blc > example1.ps + +4. alscript -f + Similar effect to 2. + +5. alscript -f -s + Silent operation: No messages are written to stderr, unless fatal. + Silent operation may be toggled by the silent_mode command in the command file. + +6. alscript -f -p < > + Make alscript work like a pipe - blocfile is read from stdin, postscript is written + to stdout. Messages are written to stderr. To supress messages include + the -s flag too + e.g. alscript -f example1.com -p -s < example1.blc > example1.ps + +Using alscript as a pipe has the advantage of allowing the blocfile to +be created on the fly by the programs msf2blc or clus2blc. For example +if we have a GCG .msf file called "pileup.msf" we can run alscript with +default shading/fonts and send the results straight to the PostScript +printer "lpr" as follows: + +msf2blc -q < pileup.msf | alscript -q -s | lpr + +See alps.c for revision history; +fprintf(std_err,"\n Version 2.0 - 23 May 1995\n"); + +*/ + +#include +#include +#include +#include "array.h" +#include "gjutil.h" +#include "version.h" + +int MAXtlen = 500; +int MAXnbloc = 500; +int MAXilen = 50; +int precis = 100; +int MAXslen = 8000; +int MAXnseq; + +void ALwritestddefs(FILE *); +int alps(char *command_file,int silent, int pipe, int single); + +main(int argc,char *argv[]) +{ + char *alps_file; + int silent; + int quick; + int i; + int pipe; + int singlepage; /* flag for EPS output */ + FILE *fp; + char *Alscript_Version = ALSCRIPT_VERSION; + + extern FILE *std_out,*std_err,*std_in; + + silent = 0; + quick = 0; + pipe = 0; + + alps_file = NULL; + + /* initialize the file streams for GJ... routines */ + GJinitfile(); + + singlepage = 0; + + if(argc > 1){ + /* check for -f option - if present, then define name for command file */ + i = 1; + while(i +#include +#include "gjutil.h" +#include "array.h" + +int Agetbloc(FILE *,struct seqdat *,int *); + +int MAXtlen = 500; +int MAXnbloc = 500; +int MAXilen = 50; +int precis = 100; +int MAXslen = 8000; +int MAXnseq; + +/* alsnum: Aim - produce TEXT commands for ALSCRIPT that will generate numbers + corresponding to a given sequence in the blockfile. + + Usage: + + alsnum < blockfile > output + + Where: + + seqnum: The number of the sequence in the blockfile. + + startnum: The number to use for the first residue in the sequence (e.g. 1). + + interval: The numbering interval (e.g. 10). + + position: Where to place the text in the alscript output (ie the Y coordinate). + + + ALL ARGUMENTS ARE REQUIRED + + + G. J. Barton (25 May 1993). + + $Id: alsnum.c,v 1.2 1998/09/17 16:54:59 geoff Exp $ + $Log: alsnum.c,v $ + Revision 1.2 1998/09/17 16:54:59 geoff + Check consistency with archive + + +*/ + +main(int argc,char *argv[]) + +{ + + extern FILE *std_in,*std_out,*std_err; + extern int MAXtlen,MAXnbloc; + extern int MAXilen,precis,MAXslen; + + int seqnum,startnum,interval,position; + int nseq; + int *nums; + int nval; + int i; + + struct seqdat *bloc; + + GJinitfile(); + + if(argc != 5){ + fprintf(std_err, + "Usage: alsnum < blockfile > output\n"); + exit(0); + } + + seqnum = atoi(argv[1]); + startnum = atoi(argv[2]); + interval = atoi(argv[3]); + position = atoi(argv[4]); + + nseq = 0; + bloc = (struct seqdat *) malloc(sizeof(struct seqdat) * MAXnbloc); + mcheck((char *) bloc,"Cannot get space for bloc"); + if(!Agetbloc(std_in,bloc,&nseq))error("Cannot read bloc file",1); + + /* have read block file */ + + nums = (int *) GJmalloc(sizeof(int) * bloc[seqnum].slen); + + nval = startnum; + + /* get the array of numbers - use blank . or - as non-amino acid character */ + for(i=1;i output +Only error messages will be output to std_err + +Default mode is interactive and prompts for filenames. + +The storage for the sequences is allocated dynamically, so the MAX_SEQ_LEN +defines in the header file "defaults.h" have no effect. If a system memory +limit is reached, then a "malloc error" message will be written and the +program will stop. Most computers should happily cope with large numbers of +long sequences. If yours doesn't, some possible solutions are outlined in +the user manual - alscript.doc. + +24 Oct 1994 - modify to remove dots and dashes from input file before writing +output file (if -n option is second argument ). + +****************************************************************************/ + +#include +#include +#include +#include +#include + +#include "gjutil.h" +#include "array.h" +#include "defaults.h" + +#define TOKENS " \t\n" + + +main(int argc,char *argv[]) +{ + struct seqdat *seqs; + FILE *fp,*fout; + int nseq; + int found; + int i,j; + char *token,*sbit; + char *line; + extern FILE *std_err,*std_in,*std_out; + char *msffile; + char *blocfile; + int quiet; + char c; + int allen; /* total alignment length */ + int nodot; + + std_err = stderr; + std_in = stdin; + std_out = stdout; + + line = GJstrcreate(MAX_INLEN," "); + msffile = GJstrcreate(MAX_INLEN,NULL); + blocfile = GJstrcreate(MAX_INLEN,NULL); + + nseq = 0; + found = 0; + quiet = 0; + allen = 0; + nodot = 0; + + if(argc > 1){ + if(strcmp(argv[1],"-q")==0){ + /* Quiet mode - read .MSF file from stdin and output block file to stdout */ + quiet = 1; + fp = std_in; + fout = std_out; + } + if(argc > 2){ + if(strcmp(argv[2],"-n")==0){ + /* set flag to remove dots */ + nodot = 1; + } + } + }else{ + /* Verbose mode - prompt for all filenames */ + fprintf(std_out,"\n\n"); + fprintf(std_out,"CLUSTAL NBRF-PIR format to AMPS Blockfile conversion\n"); + fprintf(std_out,"Copyright: G. J. Barton (1992)\n"); + fprintf(std_out,"Author: G. J. Barton (1992)\n\n"); + fprintf(std_out,"Max number/length of alignment - Defined by System\n"); + fprintf(std_out,"If you get a malloc error message - see manual\n\n"); + fprintf(std_out,"Enter CLUSTAL NBRF-PIR alignment filename: "); + + fscanf(std_in,"%s",msffile); + fprintf(std_out,"Opening: %s\n",msffile); + fp = GJfopen(msffile,"r",1); + + fprintf(std_out,"Enter Block filename: "); + fscanf(std_in,"%s",blocfile); + fprintf(std_out,"Opening: %s\n",blocfile); + fout = GJfopen(blocfile,"w",1); + } + + fprintf(fout,"\n"); + fprintf(fout,"Conversion of CLUSTAL NBRF-PIR file to AMPS BLOCKFILE format\n"); + fprintf(fout,"clus2blc: Geoffrey J. Barton (1992)\n\n"); + + seqs = (struct seqdat *) GJmalloc(sizeof(struct seqdat)); + + if(!quiet)fprintf(std_out,"Reading .pir file\n"); + nseq = 0; + while(fgets(line,MAX_INLEN,fp) != NULL){ + if(line[0] == '>'){ + /* found an identifier */ + token = strtok(&line[1]," \n"); + if(token != NULL){ + seqs = (struct seqdat *) GJrealloc(seqs,sizeof(struct seqdat) * (nseq + 1)); + seqs[nseq].id = GJstrdup(token); + if(fgets(line,MAX_INLEN,fp) != NULL){ + /* read the title line */ + seqs[nseq].title = GJstrdup(line); + seqs[nseq].seq = GJstrcreate(MAX_SEQ_LEN,NULL); + seqs[nseq].slen = 0; + seqs[nseq].seq = (char *) GJmalloc(sizeof(char)); + i=0; + while((c = fgetc(fp)) != '*'){ + /* read characters until * */ + if(isalpha(c) || c == '-' || c == '.'){ + seqs[nseq].seq = (char *) GJrealloc(seqs[nseq].seq,sizeof(char) * (i+1)); + seqs[nseq].seq[i] = c; + ++i; + }else if(c == EOF){ + break; + } + } + } + seqs[nseq].slen = i; + if(i > allen) allen = i; + ++nseq; + } + } + } + + if(!quiet)fprintf(std_out,"All %d sequences read in\n",nseq); + if(!quiet)fprintf(std_out,"Writing .blc file\n"); + + for(i=0;i%s %s",seqs[i].id,seqs[i].title); + } + fprintf(fout,"* iteration 1\n"); + for(i=0;ione +>two +>three +>four +>five +* +ABCDE +EDFTD +AAAAA +LLL +PPPPP +GGGGG +SSSSS +ABCDE +EDFTD +AAAAA +LLL +PPPPP +GGGGG +* diff --git a/sources/alscript/src/fast2blc.c b/sources/alscript/src/fast2blc.c new file mode 100644 index 0000000..1faaf08 --- /dev/null +++ b/sources/alscript/src/fast2blc.c @@ -0,0 +1,201 @@ +/*************************************************************************** + +fast2blc: A program to convert a FASTA format alignment file to an + AMPS blockfile. + + Copyright: Geoffrey J. Barton (1992,1997) + + email: geoff@ebi.ac.uk + Please see the README file for details of conditions for use of this program. + + $Id: fast2blc.c,v 1.3 1999/07/09 10:35:29 geoff Exp $ + $Log: fast2blc.c,v $ + Revision 1.3 1999/07/09 10:35:29 geoff + Change version and copyright statement to reflect 1997 status + + Revision 1.2 1998/09/17 16:55:01 geoff + Check consistency with archive + + +**************************************************************************** + +Notes: This program can be run as a pipe: type fast2blc -q < input > output +Only error messages will be output to std_err + +Default mode is interactive and prompts for filenames. + +The storage for the sequences is allocated dynamically, so the MAX_SEQ_LEN +defines in the header file "defaults.h" have no effect. If a system memory +limit is reached, then a "malloc error" message will be written and the +program will stop. Most computers should happily cope with large numbers of +long sequences. If yours doesn't, some possible solutions are outlined in +the user manual - alscript.doc. + +24 Oct 1994 - modify to remove dots and dashes from input file before writing +output file (if -n option is second argument ). + +19 April 1995 - fast2blc derived from clus2blc to read a FASTA style alignment +file. This assumes the title line is after the id code rather than on the +next line. + +****************************************************************************/ + +#include +#include +#include +#include +#include + +#include "gjutil.h" +#include "array.h" +#include "defaults.h" + +#define TOKENS " \t\n" + + +main(int argc,char *argv[]) +{ + struct seqdat *seqs; + FILE *fp,*fout; + int nseq; + int found; + int i,j; + char *token,*sbit; + char *line; + extern FILE *std_err,*std_in,*std_out; + char *msffile; + char *blocfile; + int quiet; + char c; + int allen; /* total alignment length */ + int nodot; + + std_err = stderr; + std_in = stdin; + std_out = stdout; + + line = GJstrcreate(MAX_INLEN," "); + msffile = GJstrcreate(MAX_INLEN,NULL); + blocfile = GJstrcreate(MAX_INLEN,NULL); + + nseq = 0; + found = 0; + quiet = 0; + allen = 0; + nodot = 0; + + if(argc > 1){ + if(strcmp(argv[1],"-q")==0){ + /* Quiet mode - read .MSF file from stdin and output block file to stdout */ + quiet = 1; + fp = std_in; + fout = std_out; + } + if(argc > 2){ + if(strcmp(argv[2],"-n")==0){ + /* set flag to remove dots */ + nodot = 1; + } + } + }else{ + /* Verbose mode - prompt for all filenames */ + fprintf(std_out,"\n\n"); + fprintf(std_out,"FASTA format to AMPS Blockfile conversion\n"); + fprintf(std_out,"Copyright: G. J. Barton (1992)\n"); + fprintf(std_out,"Author: G. J. Barton (1992)\n\n"); + fprintf(std_out,"Max number/length of alignment - Defined by System\n"); + fprintf(std_out,"If you get a malloc error message - see manual\n\n"); + fprintf(std_out,"Enter FASTA format alignment filename: "); + + fscanf(std_in,"%s",msffile); + fprintf(std_out,"Opening: %s\n",msffile); + fp = GJfopen(msffile,"r",1); + + fprintf(std_out,"Enter Block filename: "); + fscanf(std_in,"%s",blocfile); + fprintf(std_out,"Opening: %s\n",blocfile); + fout = GJfopen(blocfile,"w",1); + } + + fprintf(fout,"\n"); + fprintf(fout,"Conversion of FASTA file to AMPS BLOCKFILE format\n"); + fprintf(fout,"fast2blc: Geoffrey J. Barton (1992)\n\n"); + + seqs = (struct seqdat *) GJmalloc(sizeof(struct seqdat)); + + if(!quiet)fprintf(std_out,"Reading .fas file\n"); + nseq = 0; + while(fgets(line,MAX_INLEN,fp) != NULL){ + if(line[0] == '>'){ + /* found an identifier */ + token = strtok(&line[1]," -\n"); + if(token != NULL){ + seqs = (struct seqdat *) GJrealloc(seqs,sizeof(struct seqdat) * (nseq + 1)); + seqs[nseq].id = GJstrdup(token); + token = strtok(NULL,"\n"); + if(token != NULL){ + /* assign the title line */ + seqs[nseq].title = GJstrdup(token); + seqs[nseq].seq = GJstrcreate(MAX_SEQ_LEN,NULL); + seqs[nseq].slen = 0; + seqs[nseq].seq = (char *) GJmalloc(sizeof(char)); + i=0; + while((c = fgetc(fp)) != '*'){ + /* read characters until * */ + if(isalpha(c) || c == '-' || c == '.'){ + seqs[nseq].seq = (char *) GJrealloc(seqs[nseq].seq,sizeof(char) * (i+1)); + seqs[nseq].seq[i] = c; + ++i; + }else if(c == EOF){ + break; + } + } + }else{ + fprintf(std_err, + "Error: No title in sequence %s - is this FASTA format?\n",seqs[nseq].id); + exit(1); + } + seqs[nseq].slen = i; + if(i > allen) allen = i; + ++nseq; + } + } + } + + if(!quiet)fprintf(std_out,"All %d sequences read in\n",nseq); + if(!quiet)fprintf(std_out,"Writing .blc file\n"); + + for(i=0;i%s %s\n",seqs[i].id,seqs[i].title); + } + fprintf(fout,"* iteration 1\n"); + for(i=0;i +#include +#include +#include +#include +#include +#include + +#include +#include + +/* define pointers for standard streams to allow redefinition to files */ + +FILE *std_err; +FILE *std_in; +FILE *std_out; + +/* clock externs */ +clock_t start_time, end_time,initial_time,final_time; + +void *GJmalloc(size_t size) +/* malloc with simple error check */ +/* G. J. Barton 1992 */ +{ + void *ptr; + ptr = (void *) malloc(size); + if(ptr == NULL){ + GJerror("malloc error"); + exit(0); + } + return ptr; +} + +void *GJrealloc(void *ptr,size_t size) +/* realloc with error check */ +/* G. J. Barton 1992 */ +{ + ptr = (void *) realloc(ptr,size); + if(ptr == NULL){ + GJerror("realloc error"); + exit(0); + } + return ptr; +} +void *GJmallocNQ(size_t size) +/* as for GJmalloc, but returns NULL on error*/ +/* G. J. Barton 1992 */ +{ + void *ptr; + ptr = (void *) malloc(size); + if(ptr == NULL){ + GJerror("malloc error"); + return NULL; + } + return ptr; +} + +void *GJreallocNQ(void *ptr,size_t size) +/* as for GJrealloc with error check but returns NULL on error*/ +/* G. J. Barton 1992 */ +{ + ptr = (void *) realloc(ptr,size); + if(ptr == NULL){ + GJerror("realloc error"); + return NULL; + } + return ptr; +} +void GJfree(void *ptr) +/* free with error check */ +/* G. J. Barton 1992 */ +{ + if(ptr == NULL){ + GJerror("Attempt to free NULL pointer"); + exit(0); + } + free(ptr); +} + +void GJerror(const char *prefix) +/* writes error message contained in prefix and contents of errno + to std_err. +*/ +/* G. J. Barton 1992 */ +{ + if(prefix != NULL){ + if(*prefix != '\0'){ + fprintf(std_err,"%s: ",prefix); + } + } + fprintf(std_err,"%s\n",strerror(errno)); +} + +/* +error: calls GJerror +*/ +void error(const char *str,int flag) +{ + GJerror(str); + if(flag)exit(0); +} + + +char *GJstoupper(const char *s) +/* return a copy of s in upper case */ +/* G. J. Barton 1992 */ +{ + char *temp; + int i; + temp = GJstrdup(s); + i=0; + while(temp[i] != '\0'){ + temp[i] = toupper(temp[i]); + ++i; + } + return temp; +} +char *GJstolower(const char *s) +/* return a copy of s in lower case */ +/* G. J. Barton 1992 */ +{ + char *temp; + int i; + temp = GJstrdup(s); + i=0; + while(temp[i] != '\0'){ + temp[i] = tolower(temp[i]); + ++i; + } + return temp; +} +char *GJstoup(char *s) +/* return s in upper case */ +/* G. J. Barton 1992 */ +{ + int i; + i=0; + while(s[i] != '\0'){ + s[i] = toupper(s[i]); + ++i; + } + return s; +} +char *GJstolo(char *s) +/* return s in lower case */ +/* G. J. Barton 1992 */ +{ + int i; + i=0; + while(s[i] != '\0'){ + s[i] = tolower(s[i]); + ++i; + } + return s; +} + +char *GJstrdup(const char *s) +/* returns a pointer to a copy of string s */ +/* G. J. Barton 1992 */ + +{ + char *temp; + temp = (char *) GJmalloc(sizeof(char) * (strlen(s)+1)); + temp = strcpy(temp,s); + return temp; +} + +char *GJstrrename(char *old,const char *new) +/* takes old which is a pointer to a string, then replaces the contents + of the string with new, reallocating to the correct size +*/ +{ + int nlen; + nlen = strlen(new); + old = (char *) GJrealloc(old,sizeof(char) * (nlen + 1)); + old = strcpy(old,new); + return old; +} + + + +FILE *GJfopen(const char *fname,const char *type,int action) +/* a file open function with error checking. The third argument +is set to 0 if we want a failed open to return, or 1 if we +want a failed open to exit the program. +*/ +/* G. J. Barton 1992 */ +/* modified July 1995 - error message only printed if action is 1 */ +{ + FILE *ret_val; + ret_val = fopen(fname,type); + if(ret_val == NULL){ + /* GJerror(strcat("Cannot Open File: ",fname));*/ + if(action == 1){ + GJerror(strcat("Cannot Open File: ",fname)); + exit(1); + } + } + return ret_val; +} + +int GJfclose(FILE *fp,int action) +/* a file close function with error checking. The second argument +is set to 0 if we want a failed close to return, or 1 if we +want a failed close to exit the program. +*/ +/* G. J. Barton 1992 */ +{ + int ret_val; + ret_val = fclose(fp); + if(ret_val != 0){ + if(action == 1){ + GJerror("Error closing File"); + exit(1); + } + } + return ret_val; +} + + +GJFILE *GJfilemake(const char *name,const char *type,int action) +/* If action = 1 then +Tries to open the file with the given name. If successful returns +a pointer to a struct file structure with the name and filehandle. If +the open fails, or action= 0 then returns a struct file structure +with the name and a NULL filehandle */ +/* G. J. Barton 1995 */ +{ + GJFILE *ret_val; + ret_val = (GJFILE *) GJmalloc(sizeof(GJFILE)); + ret_val->name = GJstrdup(name); + if(action == 1) { + ret_val->handle = GJfopen(ret_val->name,type,0); + }else if(action == 0){ + ret_val->handle = NULL; + } + return ret_val; +} + +GJFILE *GJfilerename(GJFILE *ret_val, const char *name) +/* When passed the fval structure - renames the name part of the +file structure to name, if the handle is non null it tries to close +the file, then sets the file handle to NULL. */ +/* G. J. Barton 1995 */ +{ + if(ret_val->name != NULL) { + GJfree(ret_val->name); + ret_val->name = GJstrdup(name); + } + if(ret_val->handle != NULL) { + GJfclose(ret_val->handle,0); + ret_val->handle = NULL; + } + return ret_val; +} + +GJFILE *GJfileclose(GJFILE *ret_val,int action) +/* Closes a file named in the struct file structure returns the struct + file structure */ + +/* G. J. Barton July 1995 */ +{ + STD_FILES; + + if(GJfclose(ret_val->handle,0) == 0){ + return ret_val; + }else { + if(action == 1){ + GJerror("Error closing File"); + fprintf(std_err,"%s\n",ret_val->name); + exit(-1); + } + } + return ret_val; +} + +GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action) +/* Opens a file named in the struct file structure */ + +/* G. J. Barton October 1995 */ +{ + STD_FILES; + + ret_val->handle = GJfopen(ret_val->name,type,0); + if(ret_val->handle == NULL){ + if(action == 1){ + GJerror("Error opening File"); + fprintf(std_err,"%s\n",ret_val->name); + exit(-1); + } + } + return ret_val; +} + +GJFILE *GJfileclean(GJFILE *ret_val,int action) +/* Closes the file then sets the file pointer to NULL, then + frees the filename string */ + +/* G. J. Barton July 1995 */ +{ + if(GJfclose(ret_val->handle,0) == 0) { + ret_val->handle = NULL; + GJfree(ret_val->name); + return ret_val; + }else { + if(action == 1){ + GJerror("Error closing File"); + fprintf(std_err,"%s\n",ret_val->name); + exit(-1); + } + } + return ret_val; +} + +void GJinitfile(void) +/* just set the standard streams */ +{ + std_err = stderr; + std_in = stdin; + std_out = stdout; +} + +char *GJfnonnull(char *string) +/* return pointer to first non null character in the string */ +/* this could cause problems if the string is not null terminated */ +{ + while(*string != '\0'){ + ++string; + } + return ++string; +} + +char *GJstrappend(char *string1, char *string2) +/* appends string2 to the end of string2. Any newline characters are removed +from string1, then the first character of string2 overwrites the null at the +end of string1. +string1 and string2 must have been allocated with malloc. +*/ +/* G. J. Barton July 1992 */ +{ + char *ret_val; + ret_val = GJremovechar(string1,'\n'); + ret_val = (char *) GJrealloc(ret_val, + sizeof(char) * (strlen(ret_val) + strlen(string2) + 1)); + ret_val = strcat(ret_val,string2); + return ret_val; +} + +char *GJremovechar(char *string,char c) +/* removes all instances of character c from string + returns a pointer to the reduced, null terminated string + 11/8/1996: couple of bugs found in this routine. + the length of the string returned was too short by 2 bytes. + This is a dodgy routine since string is freed. +*/ +/* G. J. Barton (July 1992) */ +{ + char *temp; + int j,i,nchar; + nchar = 0; + i=0; + while(string[i] != '\0'){ + if(string[i] == c){ + ++nchar; + } + ++i; + } + if(nchar == 0){ + return string; + }else{ + temp = (char *) GJmalloc(sizeof(char) * (strlen(string)-nchar + 1)); + j=0; + i=0; + while(string[i] != '\0'){ + if(string[i] != c){ + temp[j] = string[i]; + ++j; + } + ++i; + } + temp[j] = '\0'; + GJfree(string); + return temp; + } +} + +char *GJremovechar2(char *string,char c) +/* removes all instances of character c from string + returns a pointer to the reduced, null terminated string +*/ +/* G. J. Barton (July 1992) */ +{ + char *temp; + int i,k,len; + k=0; + len=strlen(string); + temp = (char *) GJmalloc(sizeof(char) * (len+1)); + for(i=0;i<(len+1);++i){ + if(string[i] != c){ + temp[k] = string[i]; + ++k; + } + } + for(i=0;i<(strlen(temp)+1);++i){ + string[i] = temp[i]; + } + GJfree(temp); + return string; +} + + +char *GJsubchar(char *string,char c2,char c1) +/* substitutes c1 for c2 in string +*/ +/* G. J. Barton (July 1992) */ +{ + int i; + + i=0; + while(string[i] != '\0'){ + if(string[i] == c1){ + string[i] = c2; + } + ++i; + } + return string; +} + +/* create a string and if fchar != NULL fill with characters */ +/* always set the len-1 character to '\0' */ + +char *GJstrcreate(size_t len,char *fchar) +{ + char *ret_val; + ret_val = (char *) GJmalloc(sizeof(char) * len); + --len; + ret_val[len] = '\0'; + if(fchar != NULL){ + while(len > -1){ + ret_val[len] = *fchar; + --len; + } + } + return ret_val; +} + +/* searches for string s2 in string s1 and returns pointer to first instance +of s2 in s1 or NULL if no instance found. s1 and s2 must be null terminated +*/ +char *GJstrlocate(char *s1, char *s2) +{ + int i=0; + int j=0; + int k; + if(strlen(s1) == 0 || strlen(s2) == 0) return NULL; + while(i < strlen(s1)){ + j=0; + k=i; + while(j < strlen(s2) && s1[k] == s2[j]){ + ++k; + ++j; + } + if(j == strlen(s2)) return &s1[i]; + ++i; + } + return NULL; +} +#include +#include + + +/* GJstrtok() + +This version of strtok places the work pointer at the location of the first +character in the next token, rather than just after the last character of the +current token. This is useful for extracting quoted strings +*/ + +char *GJstrtok(char *input_string,const char *token_list) +{ + static char *work; + char *return_ptr; + + if(input_string != NULL){ + /* first call */ + work = input_string; + } + + /* search for next non-token character */ + while(strchr(token_list,*work)!=NULL){ + ++work; + } + + if(*work == '\0'){ + /* if we've reached the end of string, then return NULL */ + return NULL; + }else{ + return_ptr = (char *) work; + while(strchr(token_list,*work) == NULL){ + if(*work == '\0'){ + /* end of the string */ + return return_ptr; + }else{ + ++work; + } + } + *work = '\0'; + ++work; + /* now increment work until we find the next non-delimiter character */ + while(strchr(token_list,*work) != NULL){ + if(*work == '\0'){ + break; + }else{ + ++work; + } + } + return return_ptr; + } +} +/************************************************************************** +return a pointer to space for a rectangular unsigned character array +Version 2.0 ANSI and uses GJmallocNQ +--------------------------------------------------------------------------*/ + +unsigned char **uchararr(int i,int j) +{ + unsigned char **temp; + int k, rowsiz; + + temp = (unsigned char **) GJmallocNQ(sizeof(unsigned char *) * i); + if(temp == NULL) return NULL; + + rowsiz = sizeof(unsigned char) * j; + + for (k = 0; k < i; ++k){ + temp[k] = (unsigned char *) GJmallocNQ(rowsiz); + if(temp[k] == NULL) return NULL; + } + return temp; +} + +/************************************************************************** +free up space pointed to by rectangular unsigned character array +-------------------------------------------------------------------------*/ +void ucharfree(unsigned char **array,int i) + +{ + int k; + + for (k = 0; k < i; ++k){ + GJfree((char *) array[k]); + } + GJfree((char *) array); + +} + +/************************************************************************** +return a pointer to space for a rectangular signed character array +Version 2.0 ANSI +--------------------------------------------------------------------------*/ +signed char **chararr(int i,int j) + +{ + signed char **temp; + int k, rowsiz; + + temp = (signed char **) GJmallocNQ(sizeof(char *) * i); + + if(temp == NULL) return NULL; + + rowsiz = sizeof(char) * j; + + for (k = 0; k < i; ++k){ + temp[k] = (signed char *) GJmallocNQ(rowsiz); + if(temp[k] == NULL) return NULL; + } + return temp; +} + + +/* mcheck - check a call to malloc - if the call has failed, print the +error message and exit the program */ +/* ANSI Version - also uses GJerror routine and ptr is declared void*/ + +void mcheck(void *ptr,char *msg) + +{ + if(ptr == NULL){ + GJerror("malloc/realloc error"); + exit(0); + } +} + +/* set a string to blanks and add terminating nul */ +char *GJstrblank(char *string,int len) + +{ + --len; + string[len] = '\0'; + --len; + while(len > -1){ + string[len] = ' '; + --len; + } + return string; +} + +/* Initialise an unsigned char array */ +void GJUCinit(unsigned char **array,int i,int j,unsigned char val) +{ + int k,l; + + for(k=0;k0){ + token[i] = '\0'; + return token; + } + } +/* GJerror("End of File Encountered");*/ + GJfree(token); + return NULL; +} + +struct tokens *GJgettokens(const char *delims, char *buff) +/* This splits a buffer into tokens at each position defined by delims.*/ +/* The structure returned contains the number of tokens and the */ +/* tokens themselves as a char ** array */ +{ + char *token; + struct tokens *tok; + + token = strtok(buff,delims); + if(token == NULL) return NULL; + + tok = (struct tokens *) GJmalloc(sizeof(struct tokens)); + tok->ntok = 0; + tok->tok = (char **) GJmalloc(sizeof(char *)); + tok->tok[0] = GJstrdup(token); + ++tok->ntok; + while((token = strtok(NULL,delims)) != NULL) { + tok->tok = (char **) GJrealloc(tok->tok,sizeof(char *) * (tok->ntok+1)); + tok->tok[tok->ntok] = GJstrdup(token); + ++tok->ntok; + } + + return tok; +} + +void GJfreetokens(struct tokens *tok) +/* frees a tokens structure */ + +{ + int i; + for(i=0;intok;++i) { + GJfree(tok->tok[i]); + } + GJfree(tok->tok); + GJfree(tok); + tok = NULL; /* add this to avoid odd pointer 27/6/1997*/ +} + +char * GJtoktostr(struct tokens *tok,char delim,int s, int e) + +/* + returns a string with the tokens between s and e inclusive written to + it separated by delim + the tok structure is unmodified. +*/ + +{ + int n, i, j,k; + char *ret_val; + + n = 0; + + + if(s < 0 || s >= tok->ntok) s = 0; + if(e < 0 || e >= tok->ntok) e = tok->ntok - 1; + + for(i=s;i<=e;++i){ + n += strlen(tok->tok[i]); + ++n; + } + + ret_val = (char *) GJmalloc(sizeof(char) * n); + j = 0; + for(i=s;i<=e;++i){ + for(k=0;ktok[i]);++k){ + ret_val[j] = tok->tok[i][k]; + ++j; + } + ret_val[j++] = delim; + } + ret_val[n-1] = '\0'; + return ret_val; +} + +/* +void GJ_start_clock() +{ + extern clock_t start_time, end_time,initial_time,final_time; + start_time = clock(); + initial_time = start_time; +} + +void GJ_stop_clock(FILE *fp) +{ + extern clock_t start_time, end_time,initial_time,final_time; + end_time = clock(); + fprintf(fp,"CPU time: %f seconds\n", + ((float) (end_time - start_time))/CLOCKS_PER_SEC); +} +*/ + + +void GJ_start_clock(void) +{ + GJ_init_times(); +} + +void GJ_stop_clock(FILE *fp) +{ + GJ_show_times(fp,3); + GJ_reset_times(); +} + + +void GJindexx(int *arrin,int n,int *indx) + /* indexed heap sort - adapted from the NR routine indexx. + inarr is an integer array to sort, + indx is the returned index array + */ +{ + int l,j,ir,indxt,i; + float q; + + for (j=1;j<=n;j++) indx[j]=j; + l=(n >> 1) + 1; + ir=n; + for (;;) { + if (l > 1) + q=arrin[(indxt=indx[--l])]; + else { + q=arrin[(indxt=indx[ir])]; + indx[ir]=indx[1]; + if (--ir == 1) { + indx[1]=indxt; + return; + } + } + i=l; + j=l << 1; + while (j <= ir) { + if (j < ir && arrin[indx[j]] < arrin[indx[j+1]]) j++; + if (q < arrin[indx[j]]) { + indx[i]=indx[j]; + j += (i=j); + } + else j=ir+1; + } + indx[i]=indxt; + } +} + + + +void GJpline(FILE *fp,char c,int n) +/* print n copies of c to fp terminated by newline */ +{ + int i; + for(i=0;i n){ + for(i=0;i imax) imax = ivec[i]; + if(ivec[i] < imin) imin = ivec[i]; + } + + ret_val = (IRANGE *) GJmalloc(sizeof(IRANGE)); + ret_val->min = imin; + ret_val->max = imax; + + return ret_val; +} + +/* + +$Id: gjutil.c,v 1.2 1998/09/17 16:55:01 geoff Exp $ + +$Log: gjutil.c,v $ +Revision 1.2 1998/09/17 16:55:01 geoff +Check consistency with archive + +Revision 1.8 1998/08/11 15:38:50 geoff +Modified the copyright notice to reflect the new +ownership of this software. + +Revision 1.7 1997/06/29 00:43:57 gjb +Changes to add sysinfo calls and test of license reading routines + +Revision 1.6 1997/06/27 16:42:41 gjb +Add trlic.c test.lic and clean up gjutil.c + +Revision 1.5 1997/06/27 07:17:31 gjb +Added rlic.c linfo.h and +changes to gjutil.c to give better support for +token manipulation + +Revision 1.4 1997/05/12 11:10:53 gjb +Re-added gjutil.c and gjutil.h to repository +after deleting them + +Revision 1.2 1997/05/12 10:47:52 gjb +Modified CVS header and log position + +*/ diff --git a/sources/alscript/src/gjutil.h b/sources/alscript/src/gjutil.h new file mode 100644 index 0000000..c5aa1a2 --- /dev/null +++ b/sources/alscript/src/gjutil.h @@ -0,0 +1,109 @@ +#ifndef GJ_UTIL_H +#define GJ_UTIL_H + +/* +$Id: gjutil.h,v 1.2 1998/09/17 16:55:02 geoff Exp $ +*/ + +#include + + +typedef int GJ_LIM; /* type for limits - e.g. max buffer length */ +typedef int GJ_PEN; /* type for gap penalties */ +typedef int GJ_FLG; /* Flag type - usually just 1 or 0 */ +typedef float GJ_FLOAT; /* Single precision floating point type */ +typedef double GJ_DBL; /* a double precision floating point type */ +typedef int GJ_S_COUNT; /* small counter */ +typedef long GJ_L_COUNT; /* long counter */ + +typedef struct { /* structure to hold a filename and */ + char *name; /* associated handle */ + FILE *handle; +} GJFILE; + +struct tokens { /* structure to hold tokens parsed from */ + int ntok; /* string with strtok */ + char **tok; +}; + +typedef struct { + int min; + int max; +} IRANGE; + +#define STD_FILES extern FILE *std_in,*std_out,*std_err + +/* utility.h function definitions */ + +void *GJmalloc(size_t); +void *GJrealloc(void *,size_t); +void *GJmallocNQ(size_t); +void *GJreallocNQ(void *,size_t); +void GJfree(void *); +void GJerror(const char *); +char *GJstrdup(const char *); +char *GJstoupper(const char *); +char *GJstolower(const char *); +char *GJstoup(char *); +char *GJstolo(char *); + +FILE *GJfopen(const char *, const char *,int); +int GJfclose(FILE *,int); +GJFILE *GJfilemake(const char *name,const char *type,int action); +GJFILE *GJfilerename(GJFILE *ret_val, const char *name); +GJFILE *GJfileclose(GJFILE *ret_val,int action); +GJFILE *GJfileopen(GJFILE *ret_val,const char *type,int action); +GJFILE *GJfileclean(GJFILE *fval,int action); +void GJinitfile(void); + +char *GJfnonnull(char *); +char *GJstrappend(char *,char *); +char *GJremovechar(char *,char); +char *GJremovechar2(char *string,char c); +char *GJstrcreate(size_t, char *); +char *GJstrlocate(char *,char *); +char *GJsubchar(char *,char,char); +char *GJstrtok(char *,const char *); +void error(const char *, int); +unsigned char **uchararr(int,int); +void ucharfree(unsigned char **array,int i); +signed char **chararr(int,int); +void GJCinit(signed char **,int ,int ,char ); +void mcheck(void *, char *); +char *GJstrblank(char *, int); +void GJUCinit(unsigned char **,int ,int ,unsigned char ); +char *GJcat(int N,...); +struct tokens *GJgettokens(const char *delims, char *buff); +void GJfreetokens(struct tokens *tok); +char * GJtoktostr(struct tokens *tok, char delim, int s, int e); +void GJ_start_clock(void); +void GJ_stop_clock(FILE *fp); +char *GJstrrename(char *old,const char *new); +void GJindexx(int *arrin,int n,int *indx); + +void GJpline(FILE *fp,char c,int n); +char *GJlocaltime(void); +void GJpstring(FILE *fp,char *s,int n); + +IRANGE *irange(int *ivec, int n); + +/* +$Log: gjutil.h,v $ +Revision 1.2 1998/09/17 16:55:02 geoff +Check consistency with archive + +Revision 1.5 1997/06/27 07:17:32 gjb +Added rlic.c linfo.h and +changes to gjutil.c to give better support for +token manipulation + +Revision 1.4 1997/05/12 11:10:54 gjb +Re-added gjutil.c and gjutil.h to repository +after deleting them + +Revision 1.2 1997/05/12 10:47:52 gjb +Modified CVS header and log position + +*/ +#endif /* GJ_UTIL_H */ + diff --git a/sources/alscript/src/makefile b/sources/alscript/src/makefile new file mode 100644 index 0000000..baeb9c6 --- /dev/null +++ b/sources/alscript/src/makefile @@ -0,0 +1,61 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# These programs require an ANSI C compiler +# +# This makefile is for gcc linux computers it should need only +# minor modifications to work on other computer types, e.g. Sun +# Dec etc. This also appears to work under cygwin, though you +# have to use the --unix flag to make. +# +# +CC = gcc +MACHINE = linux +CFLAGS = -O2 -I./ -I../gjutil/ +LFLAGS = -o +ALOBJS = alscript.o alps.o ../gjutil/gjutil.o agetbloc.o ckdd.o ../gjutil/gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) fast2blc +alsnum: alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o $(LFLAGS) alsnum + + +alscript.o: alscript.c array.h ../gjutil/gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h ../gjutil/gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h ../gjutil/gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +../gjutil/gjutil.o: ../gjutil/gjutil.c ../gjutil/gjutil.h ../gjutil/gjtimes.o + (cd ../gjutil; $(CC) $(CFLAGS) -c gjutil.c gjtimes.o ; cd ../src) + +../gjutil/gjtimes.o: ../gjutil/gjtimes.c ../gjutil/gjtimes.h + (cd ../gjutil; $(CC) $(CFLAGS) -c gjtimes.c ; cd ../src) + +clus2blc.o: clus2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +msf2blc.o: msf2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +fast2blc.o: fast2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c fast2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c diff --git a/sources/alscript/src/makefile.gcc b/sources/alscript/src/makefile.gcc new file mode 100644 index 0000000..a01589e --- /dev/null +++ b/sources/alscript/src/makefile.gcc @@ -0,0 +1,63 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# These programs require an ANSI C compiler +# +# This makefile is for Sun computers +# +CC = gcc +MACHINE = gcc +CFLAGS = -O2 -I./ +LFLAGS = -o +ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o gjutil.o + $(CC) $(CFLAGS) msf2blc.o gjutil.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o gjutil.o + $(CC) $(CFLAGS) clus2blc.o gjutil.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o gjutil.o + $(CC) $(CFLAGS) fast2blc.o gjutil.o $(LFLAGS) fast2blc +alsnum: alsnum.o gjutil.o agetbloc.o + $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o $(LFLAGS) alsnum + +alscript.o: alscript.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +gjutil.o: gjutil.c gjutil.h gjtimes.o + $(CC) $(CFLAGS) -c gjutil.c + +gjtimes.o: gjtimes.c gjtimes.h + $(CC) $(CFLAGS) -c gjtimes.c + +clus2blc.o: clus2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +msf2blc.o: msf2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c + + + + + + + + + + diff --git a/sources/alscript/src/makefile.linux b/sources/alscript/src/makefile.linux new file mode 100644 index 0000000..baeb9c6 --- /dev/null +++ b/sources/alscript/src/makefile.linux @@ -0,0 +1,61 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# These programs require an ANSI C compiler +# +# This makefile is for gcc linux computers it should need only +# minor modifications to work on other computer types, e.g. Sun +# Dec etc. This also appears to work under cygwin, though you +# have to use the --unix flag to make. +# +# +CC = gcc +MACHINE = linux +CFLAGS = -O2 -I./ -I../gjutil/ +LFLAGS = -o +ALOBJS = alscript.o alps.o ../gjutil/gjutil.o agetbloc.o ckdd.o ../gjutil/gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) fast2blc +alsnum: alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o $(LFLAGS) alsnum + + +alscript.o: alscript.c array.h ../gjutil/gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h ../gjutil/gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h ../gjutil/gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +../gjutil/gjutil.o: ../gjutil/gjutil.c ../gjutil/gjutil.h ../gjutil/gjtimes.o + (cd ../gjutil; $(CC) $(CFLAGS) -c gjutil.c gjtimes.o ; cd ../src) + +../gjutil/gjtimes.o: ../gjutil/gjtimes.c ../gjutil/gjtimes.h + (cd ../gjutil; $(CC) $(CFLAGS) -c gjtimes.c ; cd ../src) + +clus2blc.o: clus2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +msf2blc.o: msf2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +fast2blc.o: fast2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c fast2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c diff --git a/sources/alscript/src/makefile.osf b/sources/alscript/src/makefile.osf new file mode 100644 index 0000000..26fb3dd --- /dev/null +++ b/sources/alscript/src/makefile.osf @@ -0,0 +1,56 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# 22 June 1993: Add alsnum program +# +# These programs require an ANSI C compiler +# +# This makefile is for Sun computers +# +CC = cc +MACHINE = osf +CFLAGS = -O2 -Olimit 1500 -I./ +LFLAGS = -o +ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) msf2blc.o gjutil.o gjtimes.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) clus2blc.o gjutil.o gjtimes.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) fast2blc.o gjutil.o gjtimes.o $(LFLAGS) fast2blc +alsnum: alsnum.o gjutil.o agetbloc.o gjtimes.o + $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o gjtimes.o $(LFLAGS) alsnum + + +alscript.o: alscript.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +gjutil.o: gjutil.c gjutil.h gjtimes.o + $(CC) $(CFLAGS) -c gjutil.c gjtimes.o + +gjtimes.o: gjtimes.c gjtimes.h + $(CC) $(CFLAGS) -c gjtimes.c + +clus2blc.o: clus2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +msf2blc.o: msf2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c diff --git a/sources/alscript/src/makefile.sgi b/sources/alscript/src/makefile.sgi new file mode 100644 index 0000000..3a2aebc --- /dev/null +++ b/sources/alscript/src/makefile.sgi @@ -0,0 +1,56 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# These programs require an ANSI C compiler +# +# This makefile is for Silicon Graphics computers it should need only +# minor modifications to work on other computer types, e.g. Sun +# Dec etc. +# +CC = cc +MACHINE = sgi +CFLAGS = -O1 -I./ -I../gjutil/ +LFLAGS = -o +ALOBJS = alscript.o alps.o ../gjutil/gjutil.o agetbloc.o ckdd.o ../gjutil/gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) msf2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) clus2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) fast2blc.o ../gjutil/gjutil.o ../gjutil/gjtimes.o $(LFLAGS) fast2blc +alsnum: alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o + $(CC) $(CFLAGS) alsnum.o ../gjutil/gjutil.o agetbloc.o ../gjutil/gjtimes.o $(LFLAGS) alsnum + + +alscript.o: alscript.c array.h ../gjutil/gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h ../gjutil/gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h ../gjutil/gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +../gjutil/gjutil.o: ../gjutil/gjutil.c ../gjutil/gjutil.h ../gjutil/gjtimes.o + (cd ../gjutil; $(CC) $(CFLAGS) -c gjutil.c gjtimes.o ; cd ../src) + +../gjutil/gjtimes.o: ../gjutil/gjtimes.c ../gjutil/gjtimes.h + (cd ../gjutil; $(CC) $(CFLAGS) -c gjtimes.c ; cd ../src) + +clus2blc.o: clus2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +msf2blc.o: msf2blc.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h ../gjutil/gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c diff --git a/sources/alscript/src/makefile.sol b/sources/alscript/src/makefile.sol new file mode 100644 index 0000000..3b585be --- /dev/null +++ b/sources/alscript/src/makefile.sol @@ -0,0 +1,63 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# 22 June 1993: Add alsnum program +# +# These programs require an ANSI C compiler +# +# This makefile is for Sun computers running Solaris 2.x +# +CC = cc +MACHINE = sol +CFLAGS = -O +LFLAGS = -I./ -o +ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) msf2blc.o gjutil.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) clus2blc.o gjutil.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) fast2blc.o gjutil.o $(LFLAGS) fast2blc +alsnum: alsnum.o gjutil.o agetbloc.o gjtimes.o + $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o gjtimes.o $(LFLAGS) alsnum + +alscript.o: alscript.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +gjutil.o: gjutil.c gjutil.h + $(CC) $(CFLAGS) -c gjutil.c + +clus2blc.o: clus2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +fast2blc.o: fast2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c fast2blc.c + +msf2blc.o: msf2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c + + + + + + + + diff --git a/sources/alscript/src/makefile.sun b/sources/alscript/src/makefile.sun new file mode 100644 index 0000000..be86d78 --- /dev/null +++ b/sources/alscript/src/makefile.sun @@ -0,0 +1,56 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# 22 June 1993: Add alsnum program +# +# These programs require an ANSI C compiler +# +# This makefile is for Sun computers +# +CC = acc +MACHINE = sun +CFLAGS = -O2 -I./ +LFLAGS = -o +ALOBJS = alscript.o alps.o gjutil.o agetbloc.o ckdd.o gjtimes.o +# +PROGS = alscript msf2blc clus2blc alsnum fast2blc + +all: $(PROGS) + +alscript: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) alscript +msf2blc: msf2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) msf2blc.o gjutil.o gjtimes.o $(LFLAGS) msf2blc +clus2blc: clus2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) clus2blc.o gjutil.o gjtimes.o $(LFLAGS) clus2blc +fast2blc: fast2blc.o gjutil.o gjtimes.o + $(CC) $(CFLAGS) fast2blc.o gjutil.o gjtimes.o $(LFLAGS) fast2blc +alsnum: alsnum.o gjutil.o agetbloc.o gjtimes.o + $(CC) $(CFLAGS) alsnum.o gjutil.o agetbloc.o gjtimes.o $(LFLAGS) alsnum + + +alscript.o: alscript.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alscript.c + +alps.o: alps.c array.h gjutil.h version.h + $(CC) $(CFLAGS) -c alps.c + +agetbloc.o: agetbloc.c array.h gjutil.h + $(CC) $(CFLAGS) -c agetbloc.c + +gjutil.o: gjutil.c gjutil.h gjtimes.o + $(CC) $(CFLAGS) -c gjutil.c gjtimes.o + +gjtimes.o: gjtimes.c gjtimes.h + $(CC) $(CFLAGS) -c gjtimes.c + +clus2blc.o: clus2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c clus2blc.c + +msf2blc.o: msf2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c msf2blc.c + +ckdd.o: ckdd.c array.h + $(CC) $(CFLAGS) -c ckdd.c + +alsnum.o: alsnum.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) -c alsnum.c diff --git a/sources/alscript/src/makefile.wat b/sources/alscript/src/makefile.wat new file mode 100644 index 0000000..f6259b3 --- /dev/null +++ b/sources/alscript/src/makefile.wat @@ -0,0 +1,60 @@ +# Makefile for ALSCRIPT, msf2blc and clus2blc programs +# +# These programs require an ANSI C compiler +# +# This makefile is for WATCOM C on a 386 or better PC. +# Use the command wmake/u to build the alscript programs. +# +CC = wcc386 +CFLAGS = +LFLAGS = /l=dos4g +ALOBJS = alscript.obj alps.obj gjutil.obj agetbloc.obj ckdd.obj gjtimes.obj +# +PROGS = alscript.exe msf2blc.exe clus2blc.exe alsnum.exe fast2blc.exe + +all: $(PROGS) + +alscript.exe: $(ALOBJS) + $(CC) $(CFLAGS) $(ALOBJS) $(LFLAGS) /fe=alscript.exe +msf2blc.exe: msf2blc.obj gjutil.obj gjtimes.obj + $(CC) $(CFLAGS) msf2blc.obj gjutil.obj gjtimes.obj $(LFLAGS) /fe=msf2blc.exe +fast2blc.exe: fast2blc.obj gjutil.obj gjtimes.obj + $(CC) $(CFLAGS) msf2blc.obj gjutil.obj gjtimes.obj $(LFLAGS) /fe=msf2blc.exe +clus2blc.exe: clus2blc.obj gjutil.obj gjtimes.obj + $(CC) $(CFLAGS) clus2blc.obj gjutil.obj gjtimes.obj $(LFLAGS) /fe=clus2blc.exe +alsnum.exe: alsnum.obj gjutil.obj agetbloc.obj gjtimes.obj + $(CC) $(CFLAGS) alsnum.obj gjutil.obj agetbloc.obj gjtimes.obj $(LFLAGS) /fe=alsnum.exe + +alscript.obj: alscript.c array.h gjutil.h version.h + $(CC) $(CFLAGS) alscript.c + +alps.obj: alps.c array.h gjutil.h version.h + $(CC) $(CFLAGS) alps.c + +agetbloc.obj: agetbloc.c array.h gjutil.h + $(CC) $(CFLAGS) agetbloc.c + +gjutil.obj: gjutil.c gjutil.h gjtimes.obj + $(CC) $(CFLAGS) gjutil.c gjtimes.obj + +gjtimes.obj: gjtimes.c gjtimes.h + $(CC) $(CFLAGS) gjtimes.c + +clus2blc.obj: clus2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) clus2blc.c + +msf2blc.obj: msf2blc.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) msf2blc.c + +ckdd.obj: ckdd.c array.h + $(CC) $(CFLAGS) ckdd.c + +alsnum.obj: alsnum.c array.h gjutil.h defaults.h + $(CC) $(CFLAGS) alsnum.c + + + + + + + diff --git a/sources/alscript/src/msf2blc.c b/sources/alscript/src/msf2blc.c new file mode 100644 index 0000000..f3aec8d --- /dev/null +++ b/sources/alscript/src/msf2blc.c @@ -0,0 +1,204 @@ +/*************************************************************************** + +msf2blc: A program to convert a GCG .MSF file into an AMPS blockfile. + + Copyright: Geoffrey J. Barton (1992,1997) + + email: geoff@ebi.ac.uk + Please see the README file for details of conditions for use of this program. + + $Id: msf2blc.c,v 1.3 1999/07/09 10:35:29 geoff Exp $ + $Log: msf2blc.c,v $ + Revision 1.3 1999/07/09 10:35:29 geoff + Change version and copyright statement to reflect 1997 status + + Revision 1.2 1998/09/17 16:55:06 geoff + Check consistency with archive + + +**************************************************************************** + +Notes: This program can be run as a pipe: type msf2blc -q < input > output +Only error messages will be output to std_err + +Default mode is interactive and prompts for filenames. + +The storage for the sequences is allocated dynamically, so the MAX_SEQ_LEN +defines in the header file "defaults.h" have no effect. If a system memory +limit is reached, then a "malloc error" message will be written and the +program will stop. Most computers should happily cope with large numbers of +long sequences. Some possible solutions to this problem are outlined in +the user manual - alscript.doc + +24 October 1994: Add -n option to delete . from alignment if found. + +****************************************************************************/ + +#include +#include +#include +#include +#include + +#include "gjutil.h" +#include "array.h" +#include "defaults.h" + +#define TOKENS " \t\n" + + +main(int argc,char *argv[]) +{ + struct seqdat *seqs; + FILE *fp,*fout; + int nseq; + int found; + int i,j; + char *token,*sbit; + char *line; + extern FILE *std_err,*std_in,*std_out; + char *msffile; + char *blocfile; + int quiet; + int nodot; +/* + std_err = stderr; + std_in = stdin; + std_out = stdout; +*/ + + GJinitfile(); + + line = GJstrcreate(MAX_INLEN," "); + msffile = GJstrcreate(MAX_INLEN,NULL); + blocfile = GJstrcreate(MAX_INLEN,NULL); + + nseq = 0; + found = 0; + quiet = 0; + nodot = 0; + + if(argc > 1){ + if(strcmp(argv[1],"-q")==0){ + /* Quiet mode - read .MSF file from stdin and output block file to stdout */ + quiet = 1; + fp = std_in; + fout = std_out; + } + if(argc > 2){ + if(strcmp(argv[2],"-n")==0){ + /* set flag to remove dots */ + nodot = 1; + } + } + }else{ + /* Verbose mode - prompt for all filenames */ + fprintf(std_out,"\n\n"); + fprintf(std_out,"GCG .MSF to AMPS Blockfile conversion\n"); + fprintf(std_out,"Copyright: G. J. Barton (1992)\n"); + fprintf(std_out,"Author: G. J. Barton (1992)\n\n"); + fprintf(std_out,"Max number/length of alignment - Defined by System\n"); + fprintf(std_out,"If you get a malloc error message - see manual\n\n"); + fprintf(std_out,"Enter MSF filename: "); + + fscanf(std_in,"%s",msffile); + fprintf(std_out,"Opening: %s\n",msffile); + fp = GJfopen(msffile,"r",1); + + fprintf(std_out,"Enter Block filename: "); + fscanf(std_in,"%s",blocfile); + fprintf(std_out,"Opening: %s\n",blocfile); + fout = GJfopen(blocfile,"w",1); + } + + fprintf(fout,"\n"); + fprintf(fout,"Conversion of GCG .MSF file to AMPS BLOCKFILE format\n"); + fprintf(fout,"msf2blc: Geoffrey J. Barton (1992)\n\n"); + + seqs = (struct seqdat *) GJmalloc(sizeof(struct seqdat)); + + if(!quiet)fprintf(std_out,"Reading .msf file\n"); + while(fgets(line,MAX_INLEN,fp) != NULL){ + if(line[0] != '\n'){ + token = strtok(line,TOKENS); + if(token != NULL){ + if(strcmp(token,"Name:") == 0){ + /* This is a seq id name */ + token = strtok(NULL,TOKENS); + seqs = (struct seqdat *) GJrealloc(seqs,sizeof(struct seqdat) * (nseq +1)); + seqs[nseq].id = GJstrdup(token); + seqs[nseq].title = GJstrdup(line); + seqs[nseq].slen = 0; + seqs[nseq].seq = (char *) GJmalloc(sizeof(char)); + ++nseq; + if(!quiet)fprintf(std_out,"%s\n",seqs[nseq-1].id); + }else if((strcmp(token,"//") == 0) || found){ + /* this signals the end of identifiers so process sequences*/ + found = 1; + if(token != NULL){ + /* find out which seq this is */ + i=0; + for(i=0;i%s %s\n",seqs[i].id,seqs[i].title); + } + fprintf(fout,"* iteration 1\n"); + for(i=0;i +#include +#include +#include + +int GJindex(char *str,char c); + +float mzcons(unsigned char *pos,int n) + +/* calculate conservation value according to Zvelebil et al for a set + of amino acids and gaps stored in string pos of length n + + Copyright: Geoffrey J. Barton (1992,1997) + + email: geoff@ebi.ac.uk + Please see the README file for details of conditions for use of this program. + + $Id: mzcons.c,v 1.2 1998/09/17 16:55:07 geoff Exp $ + $Log: mzcons.c,v $ + Revision 1.2 1998/09/17 16:55:07 geoff + Check consistency with archive + + +*/ + +{ + +/* -- conmat contains a table like that shown in the JMB paper + order of amino acids is GAP,ARNDCQEGHILKMFPSTWYBZX +*/ + static char *acids = " ARNDCQEGHILKMFPSTWYBZX"; + static int conmat[24][10] = { + {1,1,1,1,1,1,1,1,1,1}, + {1,0,0,0,0,1,1,0,0,0}, + {0,1,0,1,1,0,0,0,0,0}, + {0,0,0,1,0,1,0,0,0,0}, + {0,0,1,1,1,1,0,0,0,0}, + {1,0,0,0,0,1,0,0,0,0}, + {0,0,0,1,0,0,0,0,0,0}, + {0,0,1,1,1,0,0,0,0,0}, + {1,0,0,0,0,1,1,0,0,0}, + {1,1,0,1,1,0,0,0,1,0}, + {1,0,0,0,0,0,0,1,0,0}, + {1,0,0,0,0,0,0,1,0,0}, + {1,1,0,1,1,0,0,0,0,0}, + {1,0,0,0,0,0,0,0,0,0}, + {1,0,0,0,0,0,0,0,1,0}, + {0,0,0,0,0,1,0,0,0,1}, + {0,0,0,1,0,1,1,0,0,0}, + {1,0,0,1,0,1,0,0,0,0}, + {1,0,0,1,0,0,0,0,1,0}, + {1,0,0,1,0,0,0,0,1,0}, + {1,0,0,0,0,1,0,1,0,0}, + {0,0,0,1,0,0,0,0,0,0}, + {0,0,0,1,0,0,0,0,0,0}, + {1,1,1,1,1,1,1,1,1,1} + }; + + int concnt,it,i,j,k,l,tcnt,ibseq; + int nide, facid; + + concnt = 0; + for(j=0;j<10;++j){ + /*found: outer loop over properties + set IT to the value of the first amino acid*/ + it = conmat[GJindex(acids,pos[0])][j]; + /*loop over remaining acids, if a difference occurs then + set tcnt to 1*/ + tcnt = 0; + for(k=1;k