From e47d4dbf50727507632a3fe1c755e3141252ead7 Mon Sep 17 00:00:00 2001 From: Daniel Barton Date: Sat, 31 Aug 2013 17:42:19 +0100 Subject: [PATCH] Brief html documentation entry for RNAalifold under webServices --- help/help.jhm | 1 + help/helpTOC.xml | 2 + help/html/webServices/RNAalifold.html | 74 ++++++++++++++++++++ help/html/webServices/RNAalifoldAnnotationRows.png | Bin 0 -> 31732 bytes lib/min-jabaws-client-3.0.0.jar | Bin 538120 -> 538108 bytes 5 files changed, 77 insertions(+) create mode 100644 help/html/webServices/RNAalifold.html create mode 100644 help/html/webServices/RNAalifoldAnnotationRows.png diff --git a/help/help.jhm b/help/help.jhm index b850b79..aaaaaf8 100755 --- a/help/help.jhm +++ b/help/help.jhm @@ -37,6 +37,7 @@ + diff --git a/help/helpTOC.xml b/help/helpTOC.xml index 0d2e096..e27d41a 100755 --- a/help/helpTOC.xml +++ b/help/helpTOC.xml @@ -22,6 +22,7 @@ + @@ -65,6 +66,7 @@ + diff --git a/help/html/webServices/RNAalifold.html b/help/html/webServices/RNAalifold.html new file mode 100644 index 0000000..ac9df34 --- /dev/null +++ b/help/html/webServices/RNAalifold.html @@ -0,0 +1,74 @@ + + + + +RNAalifold Web Service + + + RNAalifold RNA Alignment Secondary Structure + Prediction Service +

+ RNAalifold is part of the Vienna + RNA Secondary Structure Prediction and Comparison Package. It was + described in 2008 by Ivo L. Hofacker, Sebastian Will, Andreas R. + Gruber, and Peter F. Stadler, RNAalifold: Improved consensus + structure prediction for RNA alignments. (BMC + Bioinformatics, 9:474, 2008). +

+

+ Example RNAalifold Output
+ RNAalifold prints a consensus alignment and mfe structure to stdout with its + associated energy. Depending on the arguments given, other information such as + alternate structures are displayed below while base pairing probabilities (-p or --MEA + options) are stored in a separate 'alifold.out' file.
+


+G_UUUCAUU___AUGACGGCCUGUGCU_UAAA__CCUCC____GAG__C________GGGUCA_G_G_UCUGAU___CUUG_______GAGAC +(.((((...........(((((((((........(((......)))............))))).).).))..................))))) (-19.16 = -11.80 + -7.36) +(.((((...........(((((((((........(((......)))............))))).).).))..................))))) [-19.32] + frequency of mfe structure in ensemble 0.765639 +(.((((...........(((((((((........(((......)))............))))).).).))..................))))) -19.16 {-11.80 + -7.36} +(.((((...........(((((((((........(((......)))............))))).).).))..................))))) { 13.20 MEA=92.14} + +Alifold.out + 6 89 9 99.2% 0.023 CG:29 GC:14 UA:9 + 4 91 11 99.1% 0.028 CG:20 GC:11 UG:2 UA:17 + 3 92 18 96.9% 0.090 CG:5 GC:2 GU:1 UG:4 AU:6 UA:25 + 35 46 3 93.3% 0.195 CG:31 GC:16 UG:2 AU:5 UA:4 + 36 45 10 93.6% 0.185 CG:16 GC:6 GU:1 UG:2 AU:8 UA:18 + . + . + . +
+

+

+ Running RNAalifold from Jalview
+ To run RNAalifold go to Webservices→RNA Structure Prediction + and choose RNAalifold Defaults to run with no arguments or + edit settings and run ... to adjust the parameters before running. + Details of all the RNAalifold parameters can be found in the + RNAalifold Manpage. + JABAWS and Jalview support a selection of the RNAalifold arguments only. +

+

Supported Arguments which give alternate structures

+

+ Partition Function (-p)
+ Calculate the Partition Function and base pairing probability matrix in addition to the mfe + structure. A coarse representation of the pair probabilities in the from of a psuedo + bracket notation, as well as the centroid structure derived from the pair probabilities + are displayed. The most likely base pairings are stored in a separate file by RNAalifold + and represented in Jalview by a bar graph annotation line labelled 'Contact Probabilities'. +

+

+ Maximum Expected Accuracy Structure (--MEA)
+ Calculate an MEA structure where the expected Accuracy is computed from the base pair + probabilities. A more detailed description is found in the + RNAfold documentation. +

+

Example RNAalifold Structure Annotation rows

+ +

+
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Ap#T5? -- 1.7.10.2